how to make CB_7K.maester_scRNA.csv

[Arsenalwins]

Dear Dr. Dou,
Thank you very much for developing and continuously improving the monopogen software, which has performed better than other similar tools in my experience.
I have a question regarding the CB_7K.maester_scRNA.csv file: could you please clarify the exact source of the reads count for each cell in this file? In your readme, you suggest exporting this value from cellranger or seurat results, but I would really appreciate a confirmation from you. Previously, I have used either nCount_RNA in Seurat or the number of lines corresponding to each barcode in the bam file as the reads count. Do you consider either of these approaches appropriate? Which method did you use when designing this file?
Thank you again for your outstanding contribution and support!

[Arsenalwins]

@jinzhuangdou Dr. Dou, if, as you suggested, that file reflects the reads count for each cell, then it seems Seurat cannot provide that information — Seurat's metrics only represent UMI counts, and I couldn't find the corresponding data in the Cell Ranger output files either. In our recent work, we have chosen to use the read count per barcode from the BAM file as the identifier; I am not sure whether this is appropriate. I would be very grateful for your guidance.