diff --git a/docs/METHODS.md b/docs/METHODS.md index c707b82d..c205dbf9 100644 --- a/docs/METHODS.md +++ b/docs/METHODS.md @@ -1,6 +1,6 @@ # OpenAMP Foundry — Methods Appendix -**Version:** 0.2.x (pipeline version); document updated for PRs #47–54 +**Version:** 0.2.x (pipeline version); document updated for PRs #47–78 **Status:** Working draft for expert review. Not a peer-reviewed publication. --- @@ -40,6 +40,15 @@ Three variant strategies were applied: Total: 383 unique variants from 5 seeds (rng_seed=2024). +> **Note (PRs #63–78):** The generation strategy above (5 seeds, rng_seed=2024) describes the +> initial variant corpus. Scoring was then improved across PRs #47–72; the current pipeline +> achieves **AUROC 0.8420** on the 87-sequence benchmark set (43 AMPs + 44 background; set +> finalized PR #66). Separately, the synthesis pool was expanded to 7 scaffold families: +> SEED-006 through SEED-009 added in expansion PRs #38–58; SEED-001 (magainin-1) re-entered +> PR #72 after the face_segregation_bonus raised its helix-score contribution. Current synthesis +> pool: **100 candidates from 7 scaffold families**. Same pipeline config (`pipeline.yaml`, +> `phase3.yaml`); wider seed set. + **Limitation:** This generation strategy produces near-seed variants. It does not explore genuinely novel sequence space. Future work should incorporate protein language model sampling or Bayesian sequence optimization.