From 37569b4ebb1ac8436875a34f6607c331a6f98aa9 Mon Sep 17 00:00:00 2001 From: j Date: Mon, 29 Jun 2026 01:25:57 +0700 Subject: [PATCH] docs: add SEED-007/005 notes to serum stability limitations in ASSAY_PREREGISTRATION MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit SEED-007: 11 AA same-length calibration edge as SEED-003; Met oxidation risk for all 4 pilot variants (Nle substitution preferred; HPLC purity mandatory). SEED-005: safety score 0.845 (lowest in panel) documented as pre-registered exclusion criterion — if HC₅₀ < 10× MIC, exclude from Wave 2. --- docs/ASSAY_PREREGISTRATION.md | 8 ++++++-- 1 file changed, 6 insertions(+), 2 deletions(-) diff --git a/docs/ASSAY_PREREGISTRATION.md b/docs/ASSAY_PREREGISTRATION.md index 86135ed2..3e319c43 100644 --- a/docs/ASSAY_PREREGISTRATION.md +++ b/docs/ASSAY_PREREGISTRATION.md @@ -137,9 +137,13 @@ TI = HC50 (µg/mL) / MIC (µg/mL) > in any resulting publication. > > Families with known model limitations (score may underestimate actual stability): -> - SEED-003 (11 AA, <15 AA calibration edge) -> - SEED-008 (13 AA, model calibrated for 18–30 AA) +> - SEED-003 (11 AA, <15 AA calibration edge; model scores 0.35–0.38 likely underestimate actual t½) +> - SEED-007 (11 AA; same length as SEED-003; Met at position 6 in all 4 pilot variants — HPLC +> purity mandatory at receipt; Nle substitution preferred for oxidation-resistant synthesis) +> - SEED-008 (13 AA, model calibrated for 18–30 AA; Trp steric effect expected to improve t½) > - SEED-009 (Pro-X protease resistance not captured by model; expected to exceed model score) +> - SEED-005 (safety score 0.845 — lowest in pilot panel; hemolysis assay at MIC/3 mandatory; +> if HC₅₀ < 10× MIC, exclude from Wave 2) ---