Removed BiocStyle dependency, by
- Added the
loadSecondaryAlignsargument totx_load_bam()so that secondary alignments can be easily discarded by setting it to FALSE. The same effect can be accomplished by inputing a scanFlag built with theRsamtools::scanBamFlag()helper, but this way it is a more streamlined feature.
- Added tx_combineTxReads() to combine reads processed by tx_reads().
- Setup pkgdown website.
- Bug fix: Loading Bed-6 gene annotations broke due to a dependency update.
- Bug fix: stringr::str_sub() update broke functions.
- Bug fix: Error processing single-end reads
- Major:
- Added ignore.strand functionality to load reads regardless if the RNA-seq library is strand-aware.
- New plotting function tx_plot_numeric(): Plots numeric variables along the transcriptomic space.
- tx_metagene_() functions have a normalization argument which yields the are under the curve to be proportional to 1(transcriptomic window length).
- Plus minor fixes.
- Fixed loading paired end BAM files when strand sign is defined by read2 ("last").
- Bugs fixed
- Minor improvements:
- Loading genome name as first word separated by spaces
- Edited main vignette tutorial "txtools"
- Added bam2txDT.R script in inst/ dir
- Patch 0.0.8.1:
- Added "splicingSite" option to tx_get_metageAtCDS() and tx_plot_metageneAtCDS().
- General Update
- Added vignette "txtools_user_guide"
- Changed tx_add_diffNucToRef() for tx_add_misincCount()
- Changed tx_add_diffNucToRefRatio() for tx_add_misincRate()
- Fix minor bugs
- 0.0.7.4 patch: Fixed a bug that incorrectly included alignments with N operations in their CIGAR strings (gaps), that didn't match the gene structure.
- Removed plotly conversion in tx_plot_*() functions. As can be easily done by the user with plotly::ggplotly()
- 0.0.7.8 patch: Fixed tx_orderDT() so that order is not affected by levels in the 'gene' factor; accordingly fix tx_unifyTxDTL() so DTs have the same txcoor order. Changing name of function tx_add_misincorpRateNucSpec() in favor of tx_add_misincRateNucSpec(), so that it conforms to other function names, former can be used as alias but has been deprecated.
- Fixed a bug detected when loading BAM files with mappings with empty sequence field
- 0.0.6.2 patch: Fixed bug related with spurious paired-end alignments, R1 and R2 order is wrong.
- Update Bioconductor remotes.
- Adding tx_core_mc() which is deprecated as alias of tx_reads().
- Fix bug in tx_complete_DT() which added refSeq before necessary.
- Uni and Multi-core functions merged into unique functions capable of using multi-cores with the parallel package.
- Bug fixed when using BED6 annotation
- Updating plotting function tx_plot_staEndCov() to remove coverage counts.
- Other minor changes
- Initial draft of package