What should I do if read length too long?

[yingeddi2008]

Hi Bao,

I am using SEED to reduce the volume of my samples now.And I noticed that you stated that the length of read should be between 21 to 100 bp. However, my samples are paired-end, and the read length for each end is 151 bp. So if I use SEED on my sample, how should I interpret the output and how trustworthy is the results?

Here is the output:

CLID    SeqID
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
ATCCATTCCAATCTATTCCATCCCAATCAATCCCATTCCAATTCATTAAAGTTGATACAATTCCAATCCAATAAACAGCACACAGAATGATTCCCTTCCATAACATTCCATACCATAACATACAATTACATAAAATACCAAAAAATACCAA
0       648870
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAATGAAACACACCTAGAACAGCACCACACTGCGCAGCAAAGACCGGCGCCGCCCCGGGGGACAAGAAGTCCAGAAACGAATGGACAACACAGAGTAACCCAAT
CCACTATTCTACTTTCTGCTTCTTTTCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTCTTTGTTTTTTTTTTTTTTCTTTCTTTTTTT
1       194982
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAACCAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAA
CAGGTGAGCTGAGTCTGAAAAGAAAAACAAAGAAAAAAGATAAAGGAAAAGCCAATTAAAAAGAAAAAGAAAGATAAAGGAAAAATACAATAAAAAAAAAAATTTTCTATAACAAACAAAAAAAAAGACACAAAGAACAAAAAAGAAGAAA
2       1279629
AAAAAAAAAAAAAAAAAAAAAAAAAAACAACCACCATGGTTTATTGTTTCTCCCCCTATGGGAGATGCAAAGACCTGTCCCCTATCCACACCTCCCCGCCCACGCGACTACGCTGCACTTCGTATTGCCTTCTCTCTTTTAAAAAAAAACA
ATCCTTGCAACTCCCATAGCTGGAGAAACCATAAACCATGGTGGTTGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTCTTCCCTTGTTTTTCTTTTTTTTTTTTTTTGTGTTTTGTGTTTTTTTTTAAAAA
3       1251316

Thanks,

Eddi

[baoe]

Hi, Eddi,

Sorry for reply late. The max read length SEED currently supports is 1000 bp. You can simply run SEED on your data.

Bao