diff --git a/tools/trim_galore/macros.xml b/tools/trim_galore/macros.xml
index 7ebf438d14..f8b8d2a632 100644
--- a/tools/trim_galore/macros.xml
+++ b/tools/trim_galore/macros.xml
@@ -1,5 +1,5 @@
- 0.6.10
+ 2.2.0
0
@@ -15,15 +15,19 @@
-
-
+
+
+
+
+
+
^[ACTGNactgn]*$
@@ -38,7 +42,7 @@
^[ACTGNactgn]*$
-
+
Instructs Trim Galore! to remove N bp from the 3' end of read 1 after adapter/quality trimming has been performed.
This may remove some unwanted bias from the 3' end that is not directly related to adapter sequence or basecall quality.
@@ -60,7 +64,7 @@
publisher = {GitHub},
journal = {GitHub repository},
url = {https://https://github.com/FelixKrueger/TrimGalore.com/fenderglass/Flye}}
-
+
diff --git a/tools/trim_galore/test-data/mec_reads_pair1.fastq.gz b/tools/trim_galore/test-data/mec_reads_pair1.fastq.gz
new file mode 100644
index 0000000000..229151a5a2
Binary files /dev/null and b/tools/trim_galore/test-data/mec_reads_pair1.fastq.gz differ
diff --git a/tools/trim_galore/test-data/mec_reads_pair1.fastqsanger b/tools/trim_galore/test-data/mec_reads_pair1.fastqsanger
deleted file mode 100644
index d44f953ce3..0000000000
--- a/tools/trim_galore/test-data/mec_reads_pair1.fastqsanger
+++ /dev/null
@@ -1,396 +0,0 @@
-@M01368:8:000000000-A3GHV:1:1101:6911:8255/1:R1:ATCTGGTT:R2:TCGCCTTA:F1:CCTA:F2:CCGC
-TTCAGGGCCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATT
-+
-GGGGGGGGGGHHHHGHGHHHHHHHHHGGGGGGHHHHGHHHHHHHHHHGHHHHHHGGHGGHHHGHHHHFHHGHHHHHHHHHGHEHEFFGHHEGGCEFGGFHHHBGHHGHHHHGHFHHHGHGHGHGGCDFDDACGGGGGGGAAFFFFFFFFFBAFFFFFB;FFFFFFADDFFFFFFFFFFEFFFFFFFFFFBFFFFFFFFFFFFFFEFFFFFFFFBFEFFFFEFE;DFFFDFBFF/9BFB
-@M01368:8:000000000-A3GHV:1:1101:14518:9998/1:R1:GTTATTAT:R2:CATCACGA:F1:TATG:F2:TGGA
-CCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGAT
-+
-GGGGGGGGGHGGHHHHGHHHHHHHGCGHHHHHHHHHHHHHHHGGGGGHHHHHHHHHGHHGFHFE5BGEEHFGGGHHHHHHHHFBHHGGGGFHGHHFGHHHHGHHHHHHGEGGGGFHFHGEGHHGGCDGDGHGGGDGGHGGCGGGHGHHH/ACDG?.1FGCDCCGCA.CC@CDCHFHGFFGGGEBFGAB//CEFBFGG.:;D;;A0AFFFFFB..:@ABFF//;BFFFFFBF/9D:A//
-@M01368:8:000000000-A3GHV:1:1101:18422:19051/1:R1:GTATCCGA:R2:CTACAAGC:F1:CATC:F2:ATTA
-GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG
-+
-GGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHFHHHHGGGGHHHHHHHHHGHHHHHHHHHHHHGGHGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHGCGGGHHHHHHHHHHHHHHHHHHHHHHGFDHGFHCFGGGGFGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF;FFFFFFFFFFFFFFFFFFFFFFFFFFFFEFBFFFFFFFFFF:FFF.
-@M01368:8:000000000-A3GHV:1:1101:25545:21098/1:R1:ATTAATTA:R2:GCCCATAA:F1:ACAC:F2:CACT
-CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATAAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGGCTTATTTAAGGGGAACGGGTGGGG
-+
-GGFEBDGEGHEGHGEGHCEGGHHCHGHHFFHHGFGAGE53FF2FAFFGDE5FFFE5GFBFGAEE1GHHHGHHHEHE3FGHF@GEGEGGHHGG3FAGFFDE?EEE3GFEGFGFGGCG?GHHHFHGGGC@DHFFHD/A@FFFGHHHHFHEFGDABFFGG/@DCE---;--..;.../9
-@M01368:8:000000000-A3GHV:1:1103:25519:6301/1:R1:AAATAAGA:R2:CTGTAATA:F1:CATC:F2:TTGA
-CGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGCATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATTTCGT
-+
-GG2CF?4FGHGHHCHGGHHBHGFFAGGHHFFHHCGHFEGCECEGHFFFHGHHGFGHFGDAFDGFFEEFEGGBECC/;>=EF?AEFFFF;9;=DAFFFFBBFFFBFFF//;BFB.:FBFFBB/BFFFF/BFFE?EFAFEF9
-@M01368:8:000000000-A3GHV:1:1104:11928:24197/1:R1:ACGTAGGT:R2:CCTCGCTC:F1:GCGA:F2:CGGG
-AAATAATGGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATA
-+
-GGGGGGGGGHHHGHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHGGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHGHHHHGHHGHHHHGGHGEEGGGGFHHFHHHGHGEHHHHHHHHHHHHHHHHHGGGGGGFGGGGGGGGFFGGGFGFGAEFEFGFEAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF@D9
-@M01368:8:000000000-A3GHV:1:1104:12288:20635/1:R1:CACTTGGG:R2:CCTACAAG:F1:GGTA:F2:CATT
-CTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGCCGCAGCATCTGTCTTTGATTCCTGCCT
-+
-GGGGFFGD5GGHGFFHGHEGGGGHHHHFFHH5FHGHHHFFHEFHHHHHHGGHHHHHGFHGGHGGGGFHGGGFBHHHFGEGHHHHGHGGGGECEHHFHHGHHFFHFGGGHHHEGHDGHHGHGHFHHHECGCBCHHGFHHFHD1GFHEFHEF?GDCFFEFE0A@;@>BD/9BFBBBBFDFFFF99D=?>DDBFF-AFFEBB9BBEFEFAFFF//-@---;;FBBFBFB///;99999BA9
-@M01368:8:000000000-A3GHV:1:1104:13832:4398/1:R1:CACGATGG:R2:GACAGATA:F1:ATCA:F2:CTGC
-AGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGACAAAAAAAAACACACAACAACCAACACCCAGTAGACTAGTGCGTC
-+
-GGGGGGGGGHFHHHHHHHHHGFFHHGGHGGGGGHHHHHHHHHHFHHHHGHHHHHGGHGHGGHGGGGGGGHGFFGGGGDGGHHHHGGGGFGGGGGCFGGEGCDGGGGGGGGGGGGEGF?;BFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF/FFFFFFC.;BFEAFFFFF;@FFBFFFFEF?/FFFFE----:...:...9....9.9...:.9//9////;/9-.-
-@M01368:8:000000000-A3GHV:1:1104:23670:4161/1:R1:ATTCAATT:R2:CGACATCT:F1:GTTA:F2:GGTT
-TATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCC
-+
-GGGGGGCGBBGGHHHHHHHHHGHHHHHHHHHHFGGHHHHHHGGFHHHHHHHGGGGGFHHHGHHHHHGHHHHGHHGGGHGGGGHHHHHGHHHHHHEGGGFHHHGFFDGEGHGHFHGHHGEGGGHHBGHGGHGHGGGGG/@FCDFDGFHGGGDFFHFGFFGGGGGHHHHGGGGGGGEGCDG?GGBFFFEE00BFFGABBBAFGGGEGEA.9FFF/./;BFBF./AAF/BFFBBF/F/BFB
-@M01368:8:000000000-A3GHV:1:1104:24927:22253/1:R1:AGCTAAAG:R2:GTAATATT:F1:TGAA:F2:GAAC
-TGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTAT
-+
-GGBGFF3EAEEEHFHHFHHHGHHHHDGHHGAEFGHHHGFCHHGHHHBGHHHAEGGHGGFHFBFHHHHHHHHHHFHH1EEHEEHH1FFDEEFCGHBGHHFHEHHHHHH1FCGHHHHEEFGEEGFFCHHH11>GHHF/FG2DGGCGAGGHGGGG@@@BEFFF?@@@FFEFFFFFF?@@FB@BB@FFFE=;==---A9EFBBB/;E;A9B/B/FFFEF/B///;/;9/A/;/9BFFFBFBB
-@M01368:8:000000000-A3GHV:1:1104:4705:7727/1:R1:CTACAAGC:R2:TCCTACTT:F1:ATTA:F2:CAGG
-TTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG
-+
-GGGGGGGGGHHHGHHHGHHHHH6GHHHHFFGGGHHHHHHHHHHHHHHHGGFGGGEGGGHHHHHHHHHHGHE3FGGGGHHHHHFHHHHHHHHHHHHGGGGGHHFHFCGFHHGGGGGGGHHGGGGHHGGGGGGHHHHGGGADCHHHHGDGGGGGGGGC@DGFHEHHHHHHGHHHHGFGDEGFGFFGGGGCFGBEGFGGGFG;C.BFFFFFFFBFBFFFF/F?.AFFF/BFFEFFFFFEFF
-@M01368:8:000000000-A3GHV:1:1104:7756:4776/1:R1:GGCCCATA:R2:CTACAAGC:F1:ACAC:F2:ATTA
-TGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGA
-+
-GGGGGGEEFHHHHGHGHHHHHHGGHHHGGGGGGHHGHHHHHGHHGHHHGGFHHHHHHHHGHHHFHHGHHGFGGGGHHHHHHHHGHGHFFHFHHHHHHGGHGFGFHHHGHG?FGHHHHHHHHHHHHHHGHGHHGHGGCDGFFHHHHGHHHHHGHHHGFHGHFHGHGHGH/@CABFFFFFFFACAFF?BBFFBFD-@-.D>FFAF.F.:9---;AADFFFFFBEFFF:9FFFFBBFFFF/
-@M01368:8:000000000-A3GHV:1:1105:11310:21761/1:R1:ATAAATAA:R2:ACTTGGGG:F1:TAGG:F2:GTAG
-TGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTT
-+
-GGGGGGGGGGHHHHHHHHHHHHGHHHHHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHGHHHGHHHGHGHHGHHHHGGHGGG=FFGEHHHHHGHFGGHHGHHHHHFHHHHHHHGGHHHHHHHGHEHHGHHHHHGGGGF9AGE?DEGFFGGGGGGGGGGGGGGFFFF/9FFBFFFFFFFEFFFBFFFFFFFFFFFFEFFF0
-@M01368:8:000000000-A3GHV:1:1105:11578:14676/1:R1:ATTGAACG:R2:GCTCCGGG:F1:TAGG:F2:CCCA
-GCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTC
-+
-GGGGGGGGGHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHFGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHHHHHHHHHGHHHHGGGGGGHGGHGHHHHHHH;CHHHHHHHHHHHHHHHGHGGGGFGGGFGGGGGGGGGGGGFGGGEGGFFFFFFFFFFFFFFFFFFFFFFFFF/FFFFFFFFFFFFFFFF0
-@M01368:8:000000000-A3GHV:1:1105:13575:19255/1:R1:TTAGTAAG:R2:GGTTCCTA:F1:TATG:F2:CTTC
-TCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGTTCCCGTGAGTGGTTAATAGGGTGATAGGCCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG
-+
-G3A111FFAEGDDBGFDHF00F01BBA00BFGEHFBFFA1FABB0FAE?CFHF1BGFHFB0011F121E//>>1F10>B?EFHGCC/?/BFG/EEGFFEE//BCFDE0?E@@B/?11?//<@?/F<<;B=.9.;.BBFFF..;A;/B/;B//;;//BBF/
-@M01368:8:000000000-A3GHV:1:1106:22927:3863/1:R1:GTATCCGA:R2:AAGACAGA:F1:CATC:F2:TACT
-GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGACTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA
-+
-GGGFGGGFGFHHHFFH4BCBGHHHHGFFF3G5BF3322EEAFHHHHHHAB5GHFHEHFHHH3BEEHFGF5@13FDF3GDGFHHFGHHHGHHHGFGGHHFCG//?/EGHHHG3F4BFFGHHHEDGFHHB/B/?FBF<@-9?BDEFFF?B--?DFFFB9FA>B;A.BFDB?AB.--@;-.FFAEFFFF9AE;DDFFFFB/9BFFFFFFFB:BF/BB/BFF//FFF-=--.99=--.9///
-@M01368:8:000000000-A3GHV:1:1106:23625:4236/1:R1:TAGCTAAA:R2:CACTTTAG:F1:GTGA:F2:TAAG
-CTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTA
-+
-GGFGFGGGEFCGGHHHHFGHGHFHGHHHHGGFGGFGHHGHGHHHHGHGFFHHGGGGGHHHHHHHHHBFHHEFHHHHHHGF?FAGHGFHHHGDGFHHGGGHGHFHHHGHHHGHHFHHGFFGFHHHGHHHHFFHHHHH/FGHHHHGHGHGDFFFE?FFF/--;;9/:BFFFBA///9----;B.//////;...---9---
-@M01368:8:000000000-A3GHV:1:1106:6455:17124/1:R1:GTATCCGA:R2:GCATTAAT:F1:CATC:F2:TAAT
-GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG
-+
-GGGGGGGGGHHHGHHGHHHHHHHHHGHHGHHGHHHGHGGGEGHHHHHHHHHHHHHGHHHGHHHGGHGGHHHHHHHHHHHHHHHHHHGHHHHHHHHGHHHHHGGCFEHHHGFHHHHGHHFFH?GGHHHHGHGHGHHCFGGGGGGGFFFFFFFFFFFFFFFFFFFFADFA?.FEFAFFFEFFFFFFFFFFFFDABFBBFFFFFFFFEFFBFFFFFFFFFFF/FFBFFFFFFADE>BFFF9
-@M01368:8:000000000-A3GHV:1:1107:14451:26748/1:R1:GTATGTTC:R2:AGCTAAAG:F1:GCCT:F2:TGAA
-TAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGA
-+
-GGGGGGGGGHGHHHHGAGHGGGGGHHHHHHHHGHHHHHGGGGGGFFHHHHHHHHHFHHHHHGGGGCHFHH3FEHHGCGGGDGHHHGFGGHHGGGGHHHHHHG/DGGGHHHHGGGGGCCHGG?CGG1GHHGHHHHBGHHHFHHGHHHHGGADAD00ECEGHEFHHFFAEFFFFFGGFBFBFFFGFFG.;B/99;BCFGGGGEFFFFFFEDA./:ADEB?FBFFFF;FFEBFBFFB./9.
-@M01368:8:000000000-A3GHV:1:1107:18714:24788/1:R1:GTATGTTC:R2:GGCCCATA:F1:GCCT:F2:ACAC
-TAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGA
-+
-GGGGGGGGGHHHHHHGGHHGGHGGHHHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHCFGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHGHHHHHHHHHHHGHHHHGGEGEGCCEGGHHHHHHG:CAFFGGGGGGBFEFGGGGHGAFFGGFGGGGGGGEFFFFFFFFFEF/EDDFFFFFFFFFBFFFFFBBFFBBF/
-@M01368:8:000000000-A3GHV:1:1107:21355:8333/1:R1:ATATTGAA:R2:GTATCCGA:F1:CGTA:F2:CATC
-GTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATG
-+
-GGGGGGGGGHHHHHHHHFHHHHHGGGGGHHHHHHFHHHGHHHHHHHGGGGGHHHHFFGHGHGGGGGGGHGGEGGGHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHGHHHFHHHHHHHHGHFFHHHHGGHGEGHHGHGFHHFHGHCEFHEGHGHHHGHHHGGGGGGCFFFFBFGGFGGGGFBG?FDGGGEGFFFFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFF.AA/9
-@M01368:8:000000000-A3GHV:1:1107:22864:15712/1:R1:AATAAGAC:R2:GTAATATT:F1:ATCA:F2:GAAC
-GATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG
-+
-GGGCEGGGGHGHHDGFHHHHHHHHHHHHHCHHGHFGBGDFGE?GHHGHFBHHFHHHHHHHHHGHFGFGHGHHGGC@DCFGHHGGGGGGGHHHHHHGGGGGFGGGGFFGGGGFADGFEFFFFFFFFFFF9;FFFF/BFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFEAECDFFFFFFFFFBFFFFFFFFFFFFFFEFBFBFFF/FFFDF-;-9;E@>-9DFFF/FFBF;-.:;.9A//
-@M01368:8:000000000-A3GHV:1:1107:24817:14233/1:R1:CTTCAGGG:R2:CTTTAGTA:F1:TCAT:F2:AGTA
-AAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACAT
-+
-CFGGGGGGGHHHFGCFGGGFFGHHCGHHHGHGHGHBFFHHHEDGGFGFGHHFFFHGGEGHFHHGHFHGFFHHHFGHHHFHACECEHHHBGFHGFHHHFHHF4GFHFHFHEHGGE/EG:A-CFGGFGADGGGGFGGGGDAAAADFA.-AFFECFDAAFFFFEFFFFBFF;D;BFFFB/;BFFFFFFFFFFFFFFBFFBF/;//;BFFFF?FFFFFFEDAFFFFFBF//:BFBACF--9/
-@M01368:8:000000000-A3GHV:1:1107:24830:14440/1:R1:AACTGTAT:R2:CTTTAGTA:F1:CCGA:F2:AGTA
-ATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACC
-+
-GGGGGGGGGHGHHGHHGHHHGGHHHHHGHGHHGDHHHHHHAGGGGFHHGHHHHHHGHHHHHHHHHHHEGAFGFHFHHHHGEGHHHHHHHHHHHHHHHHHGGHHHGEFEGGHHGHHHHHGGHGFHH/?GHHHHGFGHGFHCGGBBDAFFFEFFFFFFFFFFFFFF=CDDADDFFFFEF=BFFFEFFFBFFFFEEBF./:FFFFFFFFFFFFFFFFFFFEFFFFFFFFFFF/BFBEFFC-
-@M01368:8:000000000-A3GHV:1:1107:26478:23602/1:R1:GCTAAAGT:R2:ATCAAAGA:F1:GAAC:F2:CAGA
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATCTGTCTCTTATACACCTCTC
-+
-EGGGFGGGGGGHHDB1BGFHHHFGFGGG0F00BGBGFGGF1GBGB1BGCGAEE/FGFHGHFHHHHHBFF1B0FGGF//F//FGFGFFF/1@B1DFHHHGGEFEDHFDFAFF0B//1FDD1GFFHHDF?1BD>GF/GCAC0C@?@-;99BBB->@--;-B//99B--9-BF=A--A-:A----9AA9AFBFBBB?E@BBFFFFFFFBFFFFFBFFFBBFFF//B/////-;9
-@M01368:8:000000000-A3GHV:1:1108:11059:23181/1:R1:GTTATTAT:R2:AACTGTAT:F1:TATG:F2:CCGA
-CCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGAT
-+
-GGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHGHHGGGGHIIIHHHHHHHGHHHHGHHHGGHGGHHHHHHHHHHHHHHGGGGGHHGHHHHHHHHHHHHHHHGGGGGHHHHHFGHHHGGGGGGHHHGGGGGHGGGGHHHHHHGGGGGGHGGHEFGGGGGHGGG-ECHHHGGGGGGGGGFGFFGGGGFGGGCF?EFGG0;BFFFFB?9AAFFFFFFBF;BBFFBFFFFE?F/
-@M01368:8:000000000-A3GHV:1:1108:12233:13498/1:R1:TAGGTGCG:R2:GGTTCCTA:F1:ATAA:F2:CTTC
-TAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCCTGTCTCTTATACAC
-+
-GGGGGGGGGHHHHGGGGGHHHHHHGHGHBGFHHHHHGGGGGHHGGBFGHHGGGGGGGHHHGGGGHHGGGFHHHHHHGGGGGGHGHHGGGGGGGGGGGGGGHHHHFHHHGHGHHHHHHGHGHHGGFGGGDFFGGHHHGHGH/FHHHFHGHGHHGHHHGHHBACFHHGDFGGGGGEFGC?EGHHHB0CGHHGCGHHHHBEHGHH.::ACA;FECFGGGGGFFFEFGGGGGGGFFBBBFGBFFFEFFFFBFFFFFFBBFBFFB9..AF?FFFFFFF9
-@M01368:8:000000000-A3GHV:1:1110:14751:26540/1:R1:TAACACTT:R2:ATGTTCGC:F1:GGGG:F2:CTGT
-TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACTGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGTTGTGCACGCGATAGCATTGCGCGACGCTGGCGCCGGCGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTG
-+
-EEGFGB1112DGDGHGFGHHCG/ECEBGGHDFGHHFHGHEB1BCGFFE2EGHHGHFFFHHBFFHEFCGGHHFHHHHH111BFFHGHEHBEHGGECEFHGFHFGGDBBGHHBHHHH0GH22FGHGFFFH1BFCG0F@FGG11@GDHFG1D/1FGFHH/FCG.>0><-<@CCFFG.CC-@C?C9FFF0C---;------;9----9-9@-AAFF9B9---9-/;B9BF/9BB//B/;///
-@M01368:8:000000000-A3GHV:1:1110:15519:10008/1:R1:CTGTAATA:R2:GGTTCCTA:F1:TTGA:F2:CTTC
-CGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACC
-+
-GGGGGCBGGE?GGGHHHHHGHHBEGGHHGGGGGHHHHHHHHHHHGGHHHHGFGGGGHGHGCFGHHHGGGCGGHGHGGGGGHFGGGHHHHHHGCEFGGHHHHGGGGGGGHGG/CEGHHHHGFHHHGFHHGFFHGHGHFGGEGFDDGGBCGHHGFHH/CHHHHGHGHF0CFGGGGGGGFFFFFBFFFFFFGFFGGF?DGEDEEFEFFFF?FFFFFFFFFFFFFFFBFFF.BFFFBFBBA;
-@M01368:8:000000000-A3GHV:1:1110:16559:7507/1:R1:CATTCAAT:R2:GCCCACAC:F1:TGTT:F2:GTTC
-TTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAACTGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATC
-+
-GGGGGGGFGGGGAGHHGCHHGHHHHHHHHHHHGHHGHHHHHHHHHHHHHHHHFGGGHHHHHHHHBDHHHHHGGHFEEGHGGHHHHHHHGHHHEHFGGGGGGHHHF33FGHHG3GDDGH//EEGHHFFBF?GHHFCC@DGFCGGB?DGHAFGGHHGFH0E??<@DGHGGCFGGF?DGGGFFGGGGGGGG.C/0BBF?FBBEGG0FFF.AA9;B9/E////99;;:.//:FFFFBBF//9
-@M01368:8:000000000-A3GHV:1:1110:16700:6843/1:R1:AAATAATA:R2:GTTCCTAC:F1:GGAT:F2:TTCA
-AGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCTGTCTCTTATACACATCTCCGAGCC
-+
-GGGGGGGGGHHHHGHHHHHHHHHHHHGGGGGHHHHHGGHGHGGGGGGHHHGFFGHHGGGGHHHHHHCGG@FGHFHFEGGGGGGHGGGGDGHHHHHHHHHHHHHHGHHGHGHHGGGGGCGHEHGHHHFHHHFF.DGHHHHHHGHHHHHHGFGHGCGHGHHHHHHHHGHHHCD?:A::FCDGEGFGFFBFGBFGGGF00CBFFFF/BFBFFFFAE.FBBFBFFFFB/BFFBFFF/ADCAD
-@M01368:8:000000000-A3GHV:1:1110:18014:7856/1:R1:ATAAATAA:R2:CATCACGA:F1:TAGG:F2:TGGA
-TGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGCAAAAAAAAAATAG
-+
-GGGCGGGGGGHHHHHGFHHHHHHGHHHGGGGGFHHGHFGGHHHGGFGGGHHGGGGGHHGGGGHGGHHHGGGGGGHHGHGDGGGGGGGGB/A99FE//BBFFF.9/FF?DA=@9./9
-@M01368:8:000000000-A3GHV:1:1110:23303:11846/1:R1:ACTTGGGG:R2:GTCCTACA:F1:GTAG:F2:AGCA
-TAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGTGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTC
-+
-GGFBGGBB5AFFFGFHHGGGEGHHHFHHF3EGFHHHHEFHHGGHHHGH3EFHHHHHBDGHHE?ECHGHGHHHHHHFHHHGHHHHHHHGGHGGFHHHFHHGFFHHFEGFHHHHHHEHHFDAGHHHH/BDDFGGH1FDBGHHHHHHHCGHHHFH?0CGCF>CC=@--AFBFFB?=BFDFFF//FDB;-9A-AD-.A.A=?-9-:B/AEB/BFF.A;DDFFFBBFFFF/BBFFFFBFF9B;
-@M01368:8:000000000-A3GHV:1:1111:13195:14562/1:R1:ATCAAAGA:R2:GTATCCGA:F1:CAGA:F2:CATC
-ACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGA
-+
-GGGGGGGGGGHHGG4FDFGFGGCGGGHGHG?EGHEGFFDHFHHHHGGFE@EHHGEEEGFCCEFFGFG/EHHHHHBFBGGHFHFHEHGHEFGGGED/EFDEFFEHHBGHHCFFFDFGGHHGFFFHHHHGFG>0<0DGGFHBGHFBDFGHGFFGGAGHHGCGBHFFHHHFFFBFGGGGF/:FFFCB9F9FF0FEFE?FF0BED-D.//BF9//BBFF/BFFFEFFFFED-DCFF?.:BFA
-@M01368:8:000000000-A3GHV:1:1111:14287:20817/1:R1:GCCAACAC:R2:ATTCAATT:F1:GTTC:F2:GTTA
-CCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTCATCGCACCTACGTTCACTATTACAGGCGAACATACTTATTAAAGTGTGTTAATTAAT
-+
-GFGFGGGGGHGHHHHHHHGGGGGHGHFFCGHHGBGHFHHHHAGHHHHEHHAGFFEHEAE?GHHHHEGFFFDGHGHBFFHFHHHGHGHGHBECG@@@EHGGEGCGGGGFFGGGG?BGGGGGG.-C.FEDCFFFEFFFAF/99BEFA.:9BFB/9FFFEFFFFFFEFFF/FBBFFFFFFFFFFFB//;.@@//;DFFFF//BBFFFBF;A;DF=>B//;9B//B/;/;9//9//;B/:B9
-@M01368:8:000000000-A3GHV:1:1111:23624:14516/1:R1:GTCCTACA:R2:CGACATCT:F1:AGCA:F2:GGTT
-TAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGGGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAG
-+
-GGGGGGGBGHHHHHHGGHGGHEHGHADFHHHHEGGGHBGHFFHHEHHGHFHGG5FBEEGEGHHHGHHHEFDGHHFFGFGGHHGHHHGHBHGHFHFHHGGGGFCFBGFGE?GFBECEGDGHEHGDGCGHGC/DF@GHGFFCGGGGFFFFDD?@C@GFEC?-ADHFFHBGHFBHFG0CC//.;B0/CA.;AA.;@.AB/9BB/A/.AFFFE/BFFF///9B/FBDE9/;99/B/BFFEF/
-@M01368:8:000000000-A3GHV:1:1111:7437:24508/1:R1:ACACACTT:R2:ACATCACG:F1:TAGT:F2:ATGG
-AGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTGTCTCTTATACACATCTCCGAGCCCACGAG
-+
-GGGGGGGGGGGGGHHHHHHHHHHHHHHHHGGGFFEHGGHHHHHHHHHHHGHHGGGGGHHHHHHFHHHHGHHHHGHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHGHHGGGGGGGHGGGGGCGHHHHGHHGHHHHHHHEDHHGHGGGGGD;BEGGGFFGGGGC:CFGGGGGGGGGFGGGFFFGFFFFF;FFF;BFFFFFFFFFFFB:FFFFD=CAEEEF>-
-@M01368:8:000000000-A3GHV:1:1111:8665:25542/1:R1:TCCTACTT:R2:CTTTAGTA:F1:CAGG:F2:AGTA
-TCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTGTCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCG
-+
-GGFGFFGGGGHGH4AGGGFHHCGEE2FGHHHHHHHHHHGFHGF53FHEGGFAF5CFHHGGFGHFHHGGHHHCHHHHHHHFHHGH?EEEG1FFFEHHFHFGGHHGG?F4FBGHGHCHEF0//-9--;0CFGG?@BGDGFFFFEFBBBDA?FFBFFFF=B;-9.AA9EFFFF/;9BD.//;9/9/B:/:BFBFFBFFF/B:BFF//;/B/FFFBFF.A-BFFFBA/F//BFFFBF/;/A-
-@M01368:8:000000000-A3GHV:1:1111:9655:13916/1:R1:GGCCCATA:R2:TTAGTAAG:F1:ACAC:F2:TATG
-TGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGCGGTGTCCACGCAATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGA
-+
-GFE1EEEEGGHGHHH2FGHHHFGFGHGCFGGGEFHGGEGGHHHHGHEFBFEGHHHGHHBEHHHHGHGFHHHGGGGHHEGGGGHHHGFHGHHHH1FHHG?G/AGF1GBFGEFFHF2FDD0FG--99@=---AAABA-BF/B?A@F-9BBFF//BB/9/
-@M01368:8:000000000-A3GHV:1:1112:10272:7828/1:R1:GTATCCGA:R2:GTGCTCCG:F1:CATC:F2:GCTC
-GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT
-+
-GGGGGGGGGHGHHGHGHHHHHHHHHGHHHGGHDGHHHGGGGHHHHHHHHHHHHHHHFHGHHHHGGHGGHHHFHHHHHCGHFFHHHHHHHHHHHHHGHGEFGAEEEEHHGHHGHGHHHHHHHGHHHHHHGHGHFFBC/BA?BGGGFFFFFFFFFFFFFFFC-@DDFFFFFFFEFAFFFFFFBFBFFFFFFFFFFFFFFFFBFF->@FFFFFFFFFFBFBFAD--.99;A?////:.9A.
-@M01368:8:000000000-A3GHV:1:1112:9832:16531/1:R1:CTGTAATA:R2:GTATCCGA:F1:TTGA:F2:CATC
-CGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGGGCTGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCTTGAAGTAGGAACC
-+
-4GGGGEBGEFGHGGHGHGHHHGGHFBGFGGGGGHHEFHGEHFHFHEHHHHHGCGGGHFHHCFGHE@1EEEFFGB?EEEEHBGGGCHHGHHBEE@>EAHHFAEBDGGGCEFF?CEGFFHHHHHFHHHFHGHCGCAHHF<..C.D=0CD.GFHHHG.CAGHC:CHB0GGGFFB0:CFA.A-;9.;000CBBFFBFGA.9AA;A;EDDE.ABBFFFF9;BEFFF/BF//9/B//9:B//..
-@M01368:8:000000000-A3GHV:1:1112:9832:6701/1:R1:CGCCTGTA:R2:GCTAAAGT:F1:ATAT:F2:GAAC
-GAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGA
-+
-GGFGGGGGGHHHGGHGGHHHHHHHHHHHGHHGGGGGHHHHHHHHHHHHHHHHHHGGCGDHHHHFGGHHHGGGGGFGGHGGGGHHGGGGHHHHHHGGGGGGHHGGGGDGGFGHGGFCDCHHHHGHHHHGHHFAGFGGEHFGEG.C/FFHE?/E/?E1@EFFH?E@/EGGG//B11B//<@@C0F/<0EE/FGGFGEF1EGBBEHHCGGGHBGEHFHE0B?E--;C?CCGGG@A@GBFBBBB09?@??A-AB--ABE@=-=-9AE9A;FFFFFE=9-//;//;9FF/////;;///9B///;/B////9FFBB;--A@-;/9;9-:-/;;FFFE9BF?@;-9-99/B9F/://///99/;
-@M01368:8:000000000-A3GHV:1:1114:2404:13066/1:R1:TCCTACAA:R2:ATGGATCA:F1:GCAT:F2:CAGG
-AATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCTGTCTATTATACACATC
-+
-GGGGGGGGGHHHHHGHHHHHHHHHFFHHHHHGGGGHHHHHHHHFHHHHHHFGGHHGGHGGHHHHHHGHHFHHHHGGGGGGHHHHHHGHHHHHHHHHHGGGGGGGHH?FGHHHGGGGGGHHGGFGGHHGGGGHHHHHFGGGGFGHGHHGGGGGGGHGGGEGGHHGHHHHHHHHHGFBFFDA0FGGGFFGG0:EFGGGGGGGG;AEBF0B0BFFBFFFFFFFFFFFFFFFFFFFFFEFF0
diff --git a/tools/trim_galore/test-data/mec_reads_pair2.fastq.gz b/tools/trim_galore/test-data/mec_reads_pair2.fastq.gz
new file mode 100644
index 0000000000..229151a5a2
Binary files /dev/null and b/tools/trim_galore/test-data/mec_reads_pair2.fastq.gz differ
diff --git a/tools/trim_galore/test-data/mec_reads_pair2.fastqsanger b/tools/trim_galore/test-data/mec_reads_pair2.fastqsanger
deleted file mode 100644
index 8aeb4eb0c7..0000000000
--- a/tools/trim_galore/test-data/mec_reads_pair2.fastqsanger
+++ /dev/null
@@ -1,396 +0,0 @@
-@M01368:8:000000000-A3GHV:1:1101:6911:8255/2:R1:ATCTGGTT:R2:TCGCCTTA:F1:CCTA:F2:CCGC
-ACTCACCCACGGCGGCCATCAGCCGATACTAAGTTTGGGGTATGGTGGGGGGGATAATGAATTAGGTTGTGGGGGAGGGTTTGTGGTTGAGAGAAACACAAAAAACAATCTTATATATGGGTAGTCGTTTTGTATTGGTTTTTTGTTTTGTTTGTGTTTTGAGTGTCGGTTTAGTTCGGTGTACTAGGGGGGGTGGATGGGGTCGGCTGGTGAGGGGGTCTTAGTGTATTGAGTGTGG
-+
-A111A100000000////011110//>>/12@1@22B/////1@>21/>>/-----9/;////9////--;-;-;-----;--------9/-/-///9-;-------9//////9/////-//-/9-;-;9--/////99-;--9-:-;----/---/-----////---9-/////--;A-//////---------9/-----;-----/-/-----;--;//////////9;///-
-@M01368:8:000000000-A3GHV:1:1101:14518:9998/2:R1:GTTATTAT:R2:CATCACGA:F1:TATG:F2:TGGA
-CACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATAA
-+
-GGGGGGGGGHHHHHHHHHHHHHHHHGFHHHHGGGGGHGFHHHHHHHHHHHHHHHHHHHGHGGEHGGGGCGGGHHGGCGGGGGHHGHHHGGGGGGGG.BFFFGAGADFGAFDGFGGCFFF;DDFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFBFFFFFFFFFFFFFFFFFFF09FFFE00;BE@;DABBFFFFFBBFB00;F:9;FFBFFF9BFFFFFFFFFFFFF90/::BFFFBF0
-@M01368:8:000000000-A3GHV:1:1101:18422:19051/2:R1:GTATCCGA:R2:CTACAAGC:F1:CATC:F2:ATTA
-TTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG
-+
-GGGGGGGGGHHHGHHHHHHHHHHHHHHHHGGGGHHHHHHHHEHHHHHHHGGHHGGHGGHHHHHHHGHGGHHHGGGGGHGHHHHGGGHFHFHHHHHGGGGGHBFFCGDHHHGGGGGGHGGGGGGHHGCGGGFGHHBGGGGGFFFHEGGGGGCDCCE@EFGHHHHFHEGHGFFHHGB;ECBFGGGEFEFFGF0AFGFGFFG.;;DFFFFFFFFFF090BFFFE?FEFBBFBFFFB990BF
-@M01368:8:000000000-A3GHV:1:1101:25545:21098/2:R1:ATTAATTA:R2:GCCCATAA:F1:ACAC:F2:CACT
-GGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTTCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCAACCTATGTCGCAGTATCTGTCTTTGAT
-+
-CGGCGGGCGHHHGHHHHCGHFGHFHGFGGCDGGBD5FGFFFHFHGGGHAEBFGHHHEHGGFHBGGFHHHGGGEEFHHHFHFFHDHGHGHHHHFFHHGGFAEGFFFFFBGHHHFFHHHHHHHFHFHHFHGFHGHGA/<@EFHEHH1GGHFFFHG1>=FGHFHGF.GE//-///F??//@BCBAC<<.<<///FDFACF?FHFGC//??1B0</@GFGD/@1FG10FF>DFCFDFFFFFFFFF-BBAFFFFFFFFE@D9B;FBFFFFFFFFB9/9FFFEBF9/B/BB/BFAADDFFC-;;9-;@@9-.9/://///://///...-
-@M01368:8:000000000-A3GHV:1:1103:25465:20010/2:R1:GACATAGG:R2:AACCTAAA:F1:GTGC:F2:TAGC
-CACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGTGCACCCTATGTCCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGGTCTTGGTGGTCGTCCTCGTATTTGAACACATAATCGTG
-+
-GGGGGGGGGHHHHHHGHHHGFHHHHHHHGGGGGHHHHHGHHHHHHHHEHHHHHGHGGHHGGGHHHHGGGGGHHHHHEHFGEHHHEE14BGHHHGHGHFCE/EFGGGGGGHGGGGGG?FFGGGG.ADGGGFFDA.FFEC--;9BBBD?FFFFFFFFBBFFFFFFFFFFFFFFFFFADFDFFC-BBB;BF-.;9:B9B9/9/;9;//;:.99/;-...:.../;//////.9/////.;.
-@M01368:8:000000000-A3GHV:1:1103:25519:6301/2:R1:AAATAAGA:R2:CTGTAATA:F1:CATC:F2:TTGA
-CGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATGCTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGGGATGTCTTATTTCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGAT
-+
-FFGGGCAFGCGHGGGHGGGHHFHHHFHHGGGGGFFFHHHBHHHHG5BG5GFEEEE1FGFFGF1EGHGGG/BFHEF@EA1BE1@EED3?4DHGGGGEGHFFFDDG?DCDEG@<?@DGCAGC-;@DGFFHGCHEEFGGFGFB/;..BBB.CCE?A0FE0EBFF0FEF.;=DFFB//BFBF//;/:9BA.9FB9//;9//;9F;FF
-@M01368:8:000000000-A3GHV:1:1104:13832:4398/2:R1:CACGATGG:R2:GACAGATA:F1:ATCA:F2:CTGC
-ACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGGGGGTGCCGGCTCATTAAAAAAAGAAGATGTCGAGATGTCAGTGCGTGAGTGGAAGTG
-+
-GGGGGGGGGGGGHGGGGGGGHHHGFGGHHFGGGHGHHGHGFGECGHHHHGGGGGGGHEGFGGGHHGHFHHHHFGHFHHHHGHHHHFGGFGG3FFGGHHHHHHH2?FFGHFHHHHHHHHHBGHDGC0FGHHGHHGHHHHHHHHHHFGHHGGGGGHG-:@?FAGA:.:09CB0000;9B0.9-;---:./----.0;B00;:0;;.../90009..-..00000000-...90:.00/00
-@M01368:8:000000000-A3GHV:1:1104:23670:4161/2:R1:ATTCAATT:R2:CGACATCT:F1:GTTA:F2:GGTT
-CTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAA
-+
-GGGGGGGGGHHFHHHHHHGHGHHHHHGCHHHHGGGGHHHHHHHHHHHHHHHGHHGHGHGGHGGHGHGGHHGFGHHGHHHEHHGHHHHHGHEHEHHHGGFGGHHHFHHHGHHHHHHHGHHHHG4EGGGFCBBCA?DGGGGGGCCAFFFFFFFFFFFFFFFFFDFFF.9@--=AFFFF;FBFFBEFFF9FBFFB9/FFFFFFB/FFFFFFFBFFFF/BBFBB/FFFE?.;@FFB?FBB//
-@M01368:8:000000000-A3GHV:1:1104:24927:22253/2:R1:AGCTAAAG:R2:GTAATATT:F1:TGAA:F2:GAAC
-TAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGGGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGGGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACGAG
-+
-AFGG5GGGGHDGHHHHHHHHHHHHFHCFGC2F3BFGFGHBGHHFHEDHFGGGGGHHHG3F?GHHGGC/EFEHFGAEFFHGGAEHGGGG3EEGEEDHFHAAD@DGGGHG/AC1>GED-<<-.;C0:0C:C;GH:::::0B90;FFGEGFBFF0C.;9F0;0090;900;00FA9D.A...-9=BBFFFE/FE9;9E//BBBB.BBB///B/9/B..;.
-@M01368:8:000000000-A3GHV:1:1104:4705:7727/2:R1:CTACAAGC:R2:TCCTACTT:F1:ATTA:F2:CAGG
-TCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCG
-+
-GFGGFGGGGGHHHHFHHHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHEDEFGHFHHHHGHGHHHHHHHGHHHHHHHHFHGHHHHHGGGGGHHHGHHHHHHHHHHHGHHHHFHGHGHGGHGGGDCD.;FFGGGGGGGGFFFFFFFFFFFFF.>FFFFFF-BBEFFFFFBFFFFF-DDFFFFFFEFFFFFFFFFFFF?F///9FFFFF/;FFFFF?AADCFFEFAFFEFFFFFB/;B/A9
-@M01368:8:000000000-A3GHV:1:1104:7756:4776/2:R1:GGCCCATA:R2:CTACAAGC:F1:ACAC:F2:ATTA
-TTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG
-+
-GGGGGGGGFFHHGHHGHHGGHGHHGFHHGGGGGHHHHHHHHGFF3GGGHGGGHFDHEGHHFHHHHGFHBCGHGEGFFFFFHHHHHGHHHHHGHHHGGGGGHHGHHGGHHHGGGGGGHHECCFDHHADGGHHHFGFDC////FHHHDGFCGCDEECF?EGGFFHHFEHFGGHHFFF/A900;C.9..C0990.;CFBFGE/9.9B99/;B.99/FB///9AEF/99/9/BBF/B/B//A
-@M01368:8:000000000-A3GHV:1:1105:11310:21761/2:R1:ATAAATAA:R2:ACTTGGGG:F1:TAGG:F2:GTAG
-TAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCTCC
-+
-GGGGGGGGGHHHHHHHHGGGGGHHHHHHHHGHHHHHHHHHGHHHHHHHHGHHHHHHHHHHGGGGGHHHHHHHHHHGHHHHHHHHHHHGGHGGHHHHGHHHDGHHHHHHHHHHHHHHHHHGHHHHHGGGGGGHHHHHGHHHHHHHHFFHHHHHCCAHFHHGGFFFFFFFFFAA;BDDFFFF00.-=D.ABDA-.::AAF;F@.AAD./0000.9-;.9:00009:F090;FF000900;
-@M01368:8:000000000-A3GHV:1:1105:11578:14676/2:R1:ATTGAACG:R2:GCTCCGGG:F1:TAGG:F2:CCCA
-AACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGT
-+
-GGGGGGGGGHGGGGGHHHHHHHGHHHHGHHHHHFFFGGHGHHHHHHHHHHHHHHHHFGHHHHHHHGHHGHFHFGHHHGGGGHHHHHHHHHHHHHHFHHHHHHHGGHGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHDGGCGHHHHHHHHHHHHHFFGGFGHHGHFFBGFGB2>F@;-A@FF<-;---@-;-----;-BFF/;---9//BBFFFF//B;FF///9BF///;/;B/BBFBFFBB/;-9--///;9/:-;BFFBBB9F
-@M01368:8:000000000-A3GHV:1:1105:15502:11453/2:R1:GTCCTACA:R2:GATCACAG:F1:AGCA:F2:GTCT
-TCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACCTGTCTCTTATACACATCTG
-+
-GGGGGGGGGHHHHHHGHHHHHGGGGGFHFHHHHHHGHHHHHGHHHHHHGHFHGHGGGGGGGGHHHGGGGGGGGHHHGHGGGGGGGGGGGHHGGGFGGGFEGGGGFFFGGFGDGGGBFGFFFFFFFFFFFFFE0BFFFFFFFFFFFFFFFFFFF;BFFDFFFFBF0BFFBFFEEBFFAA009FFFFFBFFEBB0;.BFFBFFFFFFFFFFFF0;BFFFFE//090::B0000:009900
-@M01368:8:000000000-A3GHV:1:1105:16787:9771/2:R1:GTTATTAT:R2:TCCTACTT:F1:TATG:F2:CAGG
-TCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCG
-+
-GGGGGGGGGGHHHHHHHHHHHGGGGHHHHHHHHHHHHHHFHHHHHHHGGFGGHHEHHHHHAGHHGHBHHHGHHHHHHGHGHGHHHGGGGGHHHHHHHHHHHHHHHFFHHHHHGHGHGHHGGGACAFGGGGGG-AFGGFFFFFFFFFFFFFFFFFFFFF-9@AFEDFFFFFB.9-AABBBFFFEFFFFFFFFBFFFFFFEBB0BBBFFBFFBFBEDEAFFEB.ABFBFBFFFF0::/.-
-@M01368:8:000000000-A3GHV:1:1105:17564:15723/2:R1:TATGCTCG:R2:ATCCGACA:F1:CCTG:F2:TCTG
-TTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTT
-+
-GGGGGGGGGHHHHGHHGHHHHHHGHHHHHHFHHHHGGGGHHGHFHHHHHHHFHHHHHHHGHGGHGGHGHHHHFHFHHGHHHHHHHHHHHHHEHGHHHHHGGGGGHHHHGEHHGHHHHHHGHFGHFHGGAHGGHGGGCCBDFGFFAFDBDDFFFFFBDA;AAD-ADC?AFEFB-@@AAEFEFFFFF..-:.B0:FFFFFFEF0BFF09FF.0;9BFFFFF0BFFFFFF?.::-909;.9
-@M01368:8:000000000-A3GHV:1:1105:18108:12091/2:R1:TAATAGGA:R2:GTATCCGA:F1:TGAG:F2:CATC
-GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTACTGTCTCTTATACAC
-+
-GGGGGGGGGHHGHHHHFHHHHHHFHGHHHHFHFHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHGHHHHGHFHGHHGHHHHHHGHHHHHHGHHHHHGGGGGHHHHHHHHGHHHHHGFGHHHHHGHDHGHHGCA;?AAFGFEFDFFF.FFFFEBDFFFFFFFFBFFFFFFFFFEFEFFFEFFFFFFF.:BFFF0BFFFFFFFFFFFF;0FF0;BFFFFFFFFBFFFBFEFFFF0
-@M01368:8:000000000-A3GHV:1:1105:18331:16683/2:R1:TCTGGTTC:R2:TTATTATG:F1:CTAC:F2:TCCT
-CAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGGGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATATCT
-+
-GGGGGGGGGHHHHHHHHHHHHHGHHHHHFHGHHGHHHHHGGFGHHIIHHIHHHHHHHFHCFGHEEHFFHGHHFGHGHGHFHHGGGGGGEEGHHEHFHHGGHFHHFGGGGDFGBCGG?DGHGGFCGGHHECEF?CHGGDGFFGGHHC?EEGGHHHH.>AEGGGHEE-AEG0C0GCCEFFFFGEFG/;CEGGGE.:CAE.;@.@EFBFFFF/..:BFF0:BB00FBFFFFBDAFFF0;0B
-@M01368:8:000000000-A3GHV:1:1105:20577:18421/2:R1:GGGGGTAG:R2:ATGTCCTA:F1:CTAA:F2:CAAG
-ATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGCCGAAAATACCAAATGCATGGAGAGCTCCCGTGAATGGGTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTT
-+
-CFFGGGFGGHHHHHHHCEGHHCHFGHCAFEFHHHGGAEGGHHDHHHBGHBGHHH?5BGE1AEEFHHHHHGHFHBGHGEGGGGHBFHHFGHBGCFFG4EGHGG?E/FG3F3F/FGCDECEA/FGG/CEDBB//?BHEHHF0ADC@D>D9@DDCFFFFFFCFDFE.AA;/BB/.;-.;BFBBBFFBFFFFFFF/9FF?/;/BBFFFFF/;FFB/
-@M01368:8:000000000-A3GHV:1:1106:17389:18671/2:R1:CCATAACA:R2:GTATGTTC:F1:CTTG:F2:GCCT
-TAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTCTTTAGGGTTTATGACCCTGA
-+
-GGGGGGGGGHGHGHHGFGFGFHGGHHGHHHHHHHHFHHGGGGAGFBGHHHFBBGH5GDHH5EEEGEHEGFBF1DGFDGFGGGBBCEEGEHFEFDDHFBFHFD/@BGDGDHHGDCCFCD/C<->.ADDGE1GA;9.FFFFFFD-99ADFFD-.9FADF--9..;AAFEFFEAA-;.///BB:BBF/BBB//B//99/;BBB/9////F
-@M01368:8:000000000-A3GHV:1:1107:18714:24788/2:R1:GTATGTTC:R2:GGCCCATA:F1:GCCT:F2:ACAC
-TGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTAGGTCGCAGGAACTGTCCTTGT
-+
-GGGGGGEGGHHHHGGFFHHHHHHHHHHGGGGGHHHHGHHHHHHHHHHHHHFHHHHHHHHGHHHHHHGHHHHGGCGHHHHHHHHHHHHHHHGHHHHHGDGHGGHHHHHHHHGHHHHHHHHHHHGHHHHHHEHHHGHFGGGGHHHHEHFHHGHFHHF.>GGHHHGHGHGGHCFFCDFDFFFFFFAAF.FFFFFEFAFCFFA..@.AFFFC9--9;.;A///00--;A-.;.000000090
-@M01368:8:000000000-A3GHV:1:1107:21355:8333/2:R1:ATATTGAA:R2:GTATCCGA:F1:CGTA:F2:CATC
-GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCGGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG
-+
-GGGGGGGGGHHHGHHHFHHHHHHGHGHHHHHHGHHHHGGGGHHHHHHHHHFFHHFHGHHHHHHGGHGGHHHGHHHHGGHFHHHHHHHFHHGHHGEFFHFHHGGGGGGHHHFHHHHGHHHHE?FGHHHHGFGHGHHGGGDGGGGGFFFCFFFFFFFFFFFFA;DBBDFFFAFFFF;DBAFEDFFFFFEA?-9...9;9;BFFFF//BFBFFFFBFBFFFFFB/BFFF9B/EDEF-BFF.
-@M01368:8:000000000-A3GHV:1:1107:22864:15712/2:R1:AATAAGAC:R2:GTAATATT:F1:ATCA:F2:GAAC
-TAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTCTGTCTCTTATACACATCTGACGCTGCCGCCGAGGGCTCTAGTTGTGGTCTG
-+
-GGGGGGGGGGGGHHHHHHHHHHGHHHGGGGFHGHHHHGHFGFFFFGGHGGGGGCHFHFAFEDHHGGFGGGHHEGEGGHHEGGCHGHHHHGGGGGGHGF0EECGGGGFECCFFGFBGHHHHHHGGHHGHFCFEHFBEFGGADDDC=CC0CCFHG:CEHHHGHBGH:GFFGFF0CAAA/BF09BBBBBBB9CFFB0C00;000;:CFFB;:...D?>:@=:9-@...//;////..;/;/
-@M01368:8:000000000-A3GHV:1:1107:24817:14233/2:R1:CTTCAGGG:R2:CTTTAGTA:F1:TCAT:F2:AGTA
-GTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAAAGTGTGGGCTATTTAGGTTTTT
-+
-GGGGGGGGGFHHHHHHHHHHHHHHGHHHGEHGGHHGHHHHHGHHEHHGGGGEHGHHHGEHHGHHDGHHHHGGGGGFGEFGGEGHHGCEEEGHHEFGGDHGGGGAGFHHHHGGF/?//GHFFDGGGGGHAD??DC1DHHBGHHHHGHF</ACFHHCDCDE?CGEBEGFFFGF?.?DGGGGEFEFFB00CFFFB.:AFFB;/BB9BBFFBBFFFAD.;///9.A./A:FB;BF//B..
-@M01368:8:000000000-A3GHV:1:1107:24830:14440/2:R1:AACTGTAT:R2:CTTTAGTA:F1:CCGA:F2:AGTA
-GTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTAGTTAGGTTTTT
-+
-DGGGGGGGGHHHHHHHFGHHHHHHGHHHGGHGGHHHHHHGHHHFHHHGGGGGHHHHHGHHHHHHHHHHHHGGGGGHGHHFGGHHHGGGCGGGHHGGG?GGFGGGHHFHGH?FG/CDHHFAAEGGG/DHFC?-.AFGFH0CHHFH0DHHG/CDEBFHG.CA.:0C:;.FGFEFFG:E.B909BFF/;CFGBB9BBA.:CFGFFFFFFFF/;//AF?.ADEAB.9.A/;.:BFFF/9FF.
-@M01368:8:000000000-A3GHV:1:1107:26478:23602/2:R1:GCTAAAGT:R2:ATCAAAGA:F1:GAAC:F2:CAGA
-ACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGCGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTTAGCCTGTCTCTTATACACATCTG
-+
-GGGGGGGGGGGFHHEFGHHHGGGGGGHHHCGGEFHFGGGFHHHHHGGGEGGHHFHEEGEG/EEEE/?/EHFHH3GGH4EGHHCB01CGHGHGD//<@CGGBFFDGHHHH0FEHHGEGFHCD11>0=GGHGCCFGHGBGGD0GGHC0C.@?ACEEC/.EDEB?BFFCFB9CFF090BBCBFBF0CCB0B00A.9E.B/9BA=-..B9FBF//;BBBBFFB:./9/9/9;B//9BF/;B/
-@M01368:8:000000000-A3GHV:1:1108:11059:23181/2:R1:GTTATTAT:R2:AACTGTAT:F1:TATG:F2:CCGA
-ATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACC
-+
-GGGGGGGGGHHHHHHHHHHHGHHHHHHHHGHHHHHHHHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHHHHHHHFHHHHHHHHHHHHHHHHGHGHEHHGFCGGGHHHHHHHHHHHHHHHFGHHHHHGHGHGHHGGGBFGFFFFEAFFFA.BFFFEFDDFFFFFFFFFFEEFF;D;EFFFFFF0BFFFADDFFFFFBFBFFFFFBB0FFFF0::0BF000BFFFBBF0EDFF@
-@M01368:8:000000000-A3GHV:1:1108:12233:13498/2:R1:TAGGTGCG:R2:GGTTCCTA:F1:ATAA:F2:CTTC
-GGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACTGTCTCTTATACAC
-+
-GGGGGGGGGHHHGHHHHHHBGHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHGHHHHHGGHHGHHHHHHGHHHHHHHGGHHHHGGGGGHHHHHHHHHHHHHGHGHHHHHHGHGHGGHGGGGAGGFGGGGGGGGFFFFFFFFFFDFFFFDFFFFFFFFF>.AEDEFFFFF?E-DAB9FFFFFFFEF:FBFB009:0FFFF9BFFBFFFFBB0.9A=-/0BFF0BBFFBFF:90:
-@M01368:8:000000000-A3GHV:1:1108:14328:14751/2:R1:ATAATAGG:R2:GTATCCGA:F1:ATGA:F2:CATC
-GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATCTGTCTCTTATACA
-+
-GGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHDHHHHGGGGHHHHHHHHHHHHGHHHHHHHHHGGHGGHGGHHHHHGHHHHHHHHHHHHHHHHFHGHHHHHGGGGGHHHHHGHGHHHHHHG?FHHHFHGHGHGHHGGGGGGGGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFAFFE.ADEFFFFFFFADFFFFF///BBFF/FFFFFBFAFFFFBB///;9:BBFFFBF9B9//:/
-@M01368:8:000000000-A3GHV:1:1108:16350:22621/2:R1:TTAGTAAG:R2:CATCTGGT:F1:TATG:F2:TCCT
-CTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATAT
-+
-GGGGCGGGGHHHHHHHHGHHHHGHGHHHHGGGGHHHHHHHHHHHIHHHGHHHHHHGGHGGHHHHGHGHGHHHHHHHHHHHGHHHHHHGGHHHHGGGGGHHHHHHHHHHHHHGHGHHHGHHGGGGGHHGGGDADDGFFGGGGFFFFFFFFFDFFFDFFFFFFFFFFFAFAEFEDFFFFFFEE-AAFFFFFBFFFFF0FFFBFF;AFFFFFFFFFFFFFFFFFFFFFAB9BFFFFFBFFF
-@M01368:8:000000000-A3GHV:1:1108:21791:23272/2:R1:CTTTAGTA:R2:CATAACAC:F1:AGTA:F2:TTGG
-GGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCT
-+
-GGGGGGGGGHHHHGGHHHHHHHGGGEGGHHFGDHGHHHHHHGGHHH2GGHHHGHHGGGGFGFBFHHHGGGGHGHGHHHHHHHHHHGHGHHGHHGGHGGHHHHGGFHGGGHFHGHHHHHGGHHHGHGFCGFFDGGG/FCFHBGHHHHHHHGHCHHHHGGGHGHGHGCFF;>@DFFBFFFFFFDFFFFFFFF;;.DDAA;FFF;BB;C@..9::BBFBBA;-:.::FBB///;/////:/
-@M01368:8:000000000-A3GHV:1:1108:23314:26089/2:R1:ATTGAACG:R2:CATAACAC:F1:TAGG:F2:TTGG
-GGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCC
-+
-GGGGGGGGGHHGDHHHHHFHHHHGGGGGFHFFGFFHEGHHHHHHHHFGHFHHHHDEHHHHHHGHFHHGGGGHHGFHHHHHHHHHHHHHHHHHHF?EEFCHFGFHHEFFFHGFHHGHFHFHHHFFHAG??GHACGGDGHGGBFHHHHHHHGHFGHGHGEGHGCFFF@CD-BDDFFFFFFFFDDFFBBFFD-B-A.DFFA.AFEFF-;-..:..BFFFFFA-../9FFF//B/BBBBFF/
-@M01368:8:000000000-A3GHV:1:1108:25084:18127/2:R1:TTATTATG:R2:CCCTTAAA:F1:TCCT:F2:TAAG
-CATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAG
-+
-GGGGGGGGGGGHHHHHHHHFHHHHHHHHHHHHHHGGHHGHHHHHGGGGFHHHHHFHHHHHHHHHGGHHHHHCGGHGHHGGGGGGGHHGGGGGGGGHHHGHHGGGGGGGGGGGGGGGG@CDE?EFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFFFE;DFBFFFFFFFFFFFFFFFFFFFAFFFFFFFBFFE0BBBFFFFFFFFFFBFFFF9BB0:0:B;
-@M01368:8:000000000-A3GHV:1:1108:7626:10048/2:R1:ATCAAAGA:R2:AACCTAAA:F1:CAGA:F2:TAGC
-CACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGGAGATCTCTGGGGTCGGCGGATTT
-+
-GGGGGGGGGHHHHHHHHHHHHHHHHHHHGGGGGHHHHHHHHFGHHHHHHHHHHHHGHHHHGGFHHHFGGGGHHHGHHHHHHHHHHHFGHHHHHGHGHGHHGCGCGGFHHHGGGGGGGGGGGGGGGGGGGFFFAFFFFFFAA-AFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBBFFDFFFFFB-<>;>@BE<:000.0.0/0<00<0:>..-<<<---.0
-@M01368:8:000000000-A3GHV:1:1109:11270:12255/2:R1:AATCAAAG:R2:CTACCTCA:F1:ACAG:F2:GGGT
-ATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGGAGATT
-+
-GGGGGGGGGHHHHHFHHHHGGGGHHHHHHHHHHHHHHHHHHHHGHGGHGGHHGHHHHHGHHHHHHHHHFHHHHHGHHGHHHHHGGGGGHHHHHHHHGHHHHHH?FHHHHHGHGHGHHGGGGGGGGGGGGGGGGGGGGFFFFFFFFFFFFFFFFECADFFFF.DEBFFFFFF;D.B;.ED.FFFFFFF-.:00009BBF
-@M01368:8:000000000-A3GHV:1:1109:24217:19077/2:R1:AAAGTGAA:R2:TAATAGGA:F1:CTGT:F2:TGAG
-CAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGAACCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTTCTGTC
-+
-FEGGCFGFGHHHHFHHFHHHHHHGGEEEFGHGFFAGFFEEAEGGHHBEEFAFGAEF1G3GFGGEEF@EAGGHGFF?ECGGHEEEFGGHHHHFHHFHHGFHHHC2DAGHGG00-BFFA9FFFFFFFFFFFBFAFFAFFFFFBFFFFFFF/9BFFDFFDDFF-9@CDAD;:AFF/;B//....;;///;
-@M01368:8:000000000-A3GHV:1:1109:27103:7153/2:R1:GTAATATT:R2:NTACTTCA:F1:GAAC:F2:GGGT
-NTAAAACCTNAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCANGGNAGCTCTCCNNGNNNNTGGTATTNTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACCTGTCTC
-+
-#AAFFGGGG#BAFFAGHHHGGGGHGHHHHFHHHHHHHGHHHHHHHEGFGGHHBGHGHHBGFFEG2GHHFHFHHHGHHGHFH#/B#??FFGHHH##?####????FHG#??CFGGGEF>CGAGAGGGFGAA@@FFFFFFFFB=@@FF?FF;FFFF@@-@-AFEF?BB/BF?E-BBFFF;:BB/BFFFB/BFFFBFBFBBFFFFFB/FFFBB/;9A@@BFFBFFFF/BBFFFFFFE/:9/
-@M01368:8:000000000-A3GHV:1:1109:8763:7859/2:R1:TAGTAAGT:R2:GGTTCCTA:F1:ATGT:F2:CTTC
-GGGTCATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAG
-+
-GGGGGGGGGHHHHHHHCHFAFHHDGEGFHHHHHHHHFHHHHFHHHHHHGHGGHGGHHHFHGHHHHHFFHHHHHHBGHFFHGHGHHHHHGGEGGHHHHHGHHHHHHHHHGFGHHHHGHGHGHHCCGGGGGGGGGGAAGF?FFFFBFA@=BDDFF?-;FFF;@CFFEFEFFFFFFFFFBFDFFFFFFFFFFFFFFFFFFFFFF:FFFF/BF/FFFEFF?DECAFEFFFFFE:FFFFFFFB
-@M01368:8:000000000-A3GHV:1:1110:12808:18367/2:R1:AATTAACA:R2:GCCCACAC:F1:CACT:F2:GTTC
-CCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACAGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTCTT
-+
-GGGGGGGGGHHHHHHHHHGGGGGGHHHHFHHGHHHHHHHHHHHHHGHHHGGHHHH3GFHEFHHHHHGHHGFHHHHHFGHHHHHGHGHGGHGGGGGGGHHHGGCGGGGHHGGGGGGGGGGGGGGGGGGGF-BAEFEBEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFFAFEFDFFFFFFFFFF0BFBED=@DFFFFFFFFFEFFFFFBFAFB;0:;F0
-@M01368:8:000000000-A3GHV:1:1110:14751:26540/2:R1:TAACACTT:R2:ATGTTCGC:F1:GGGG:F2:CTGT
-ATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGGTTGGGCTATTTGGGTTTTTTGACCCTGAAG
-+
-GGGGGGGGGHHHHGGFHGGHGGCHHHHHHGHHHGHHGEGGGHHHHHHHHHHEFHHHHHHGEE?FGHGEHGGHHHGGGGGGEHHGGGGHHGGGEGHHHGHGGGDFGHHGHGGGGGCGAFFCCDFHHHH1CGGHBHFHFCGFFCDFHGCCE.CC:EGFHHCGHHGCF?FGGGFGGG;B9;FFFFFFEGEBF0;;;0;CFBFFF?D.DE.:..99-...;;//9A.;DE.9-./:FB.;//
-@M01368:8:000000000-A3GHV:1:1110:15519:10008/2:R1:CTGTAATA:R2:GGTTCCTA:F1:TTGA:F2:CTTC
-GGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAG
-+
-GGGGGGGGGHHHGHHHFHHBGHHHGGGGHHHHHHGHHHHHHHHHHHHHHHGGHGGHHGHHHGGBGHHHHHHHHHHHHHHHHHGGFHHHGGGEGHHHHHHHHHHHHHHHGHHHHHHGHGHGHHGGGGGGDGGGGGGGGGBFFFFFFFFFFFFFFFFFFFECB-=BFFFFFFFFBB?E-A::FFBFFFFFBFFFFFFFFFFFFBFFEFFF;0BFF0:FE?FFFFBFFFFFFFFFFFFFF0
-@M01368:8:000000000-A3GHV:1:1110:16559:7507/2:R1:CATTCAAT:R2:GCCCACAC:F1:TGTT:F2:GTTC
-CCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAAT
-+
-GGGGGGGGGHHHHHHHHHGGHGGHGHFHHHHFHHHHHHHHHHFHHGHHHHGHHHHHGGEEGHHHHHHHHHHHHHHHHHHHHHHGHGHGHHGGGGGGGHHGGGFGAGFGGGGGGGGGGGDDDFAGGFFFFCFAFEEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFFFFFFFFFFFFFFE..@BFFBD./;:0FFFFF09B/AFCA=-99BFFBFFFFFFFB:BFBFFBFB9:
-@M01368:8:000000000-A3GHV:1:1110:16700:6843/2:R1:AAATAATA:R2:GTTCCTAC:F1:GGAT:F2:TTCA
-GGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTCTGTCTCTTATACACATTTGTCGCTC
-+
-GGGGGGGGGHHGHHHGGHFHHHGGGGGHHHHHHHHHHGHHHHHHHHHHHGGHGGHHHHHHHGBGFFHHHHHHGHHHHHHHHGHHHHHGGGGGHHHHHHHHHHHHHHHFHHHHGHCG/GGHHGGGGGGAFGEEADAGGDFFFF00D>CFDDFFFB?AF?B;-@>.FEDFFFFF0BF-BDFFFFE00B0BF0FFFF:BFA0B0:BBFBBFFFFF0B0:000<00FDFFFFFFFFFFFBFFFFFFFFFFF/FFF/BFFFFFFFBA.?@;FFBDFFFF/
-@M01368:8:000000000-A3GHV:1:1111:7437:24508/2:R1:ACACACTT:R2:ACATCACG:F1:TAGT:F2:ATGG
-TCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTCTGTCTCTTATACACATCTGACGCTGCCGAGA
-+
-GGGGGGGFGHHHHHHHHHHHHHGHHHGFHHHHGGGGDHHHHHHHHGFHHHHHGHHHHHHGHFHGHHGGGDFGDFFGGGFFGFFGHGHHHGGC?AD-:A-AFFGGG-?9EFEFGGFFBFEFCFFFFFFFBBBFFFFFFFFFFFFFFFBBFFFFFFFFFFFFFFFFFFEFFFFFFBFFFFEFEFFFF;CF-FFFFFFFF/BFFFFFFFFFFFFEFBFFFB/FFFFFFF//9A..;F--@9
-@M01368:8:000000000-A3GHV:1:1111:8665:25542/2:R1:TCCTACTT:R2:CTTTAGTA:F1:CAGG:F2:AGTA
-GTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGACAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTA
-+
-GGGGGGGGGHHHHHHHFHHHHCHHGDGGGGHGGHHHHHHHHGHGHHHGGGGGHFHHHHFHHHFFHHHHHFGCGGEHFHHFGGHFHGGGGGGHHFEEEAFEFFDFHGHHHHGGGGGGGHH0DGGGGCGHCC-DFFF.
-@M01368:8:000000000-A3GHV:1:1113:5741:16959/2:R1:TAGTAAGT:R2:GTATCCGA:F1:ATGT:F2:CATC
-GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG
-+
-GGGGGGGGGHHHHHHHGHHHHHHHHGHHHHGHDGHHHGGGGHHHHHHHHHHHHHHHGHHHHHGGGEGGHHHHFHHHHHHHHHHHHHHHHHHHHHHGHHGHHGGGGGHHHHHHHHHHHGHHHFHHHHHHGHGHGHGGGGCGGFGGFFFFFFFFFFFFFBFFFFFFFFFFFFFFAFFFFEAEFFFFFFFFFFF9BFFBFFFFFFFFFFFFFFBFFFFFFFFFFFFFFFFFFADAB-/BF.
-@M01368:8:000000000-A3GHV:1:1114:10130:11959/2:R1:CGTTCCCC:R2:ATCAGAGA:F1:TTAA:F2:CAGA
-ACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGGAGAGTTCTGTGGGGCCGGGGATCCTTATAAAAAAAATAGA
-+
-GGGGGGGGGGHHHHHGGHHHGGGCGGHGHGGGGHHGGGGHHHHHHGGGEGGHHHFGGGGG?E1FE?/EEHHHHHGHHGHHHHGHFHGHGHHGDGGFG2FF2?GHHHHHGCCCFHGHGHHHHGHHFEHHFGHHGHH<1=DGHHHGHHGHGAGAEEDG.CGCGHC0CGBFHGFBBF0ABDDEFF@?--:BB@.;:BF;0.0:0.0:000:BF.-.------.9/;000::0;0:--.000
-@M01368:8:000000000-A3GHV:1:1114:14540:5315/2:R1:CTTTAGTA:R2:CACTTGGG:F1:AGTA:F2:GGTA
-CTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCC
-+
-GGGGGGGGGHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHHGHHHHHHHGHHHHFHFHHHGGGGGHHHHHHHHHHHHHGHHHHHHHHGGGGGGHHHFHHHGHHHHHHHHHHHHHHHHHHGHGHHHGGGFGHHHHHHHHFHHHHHF?1FHHGHGHGHGHHGGFFFFDBFBE;BCC.:BFFFFFFFFFFFFFF;AFFFFF-=-.AEDEFFFFF..9A;9FFFF0FFFFE00FFF0:BA.
-@M01368:8:000000000-A3GHV:1:1114:15066:16302/2:R1:TAAATAAG:R2:TTATTATT:F1:ACAT:F2:ATGT
-CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGTGG
-+
-GGGGGGGGGHHHHHHHHHHHHHHHDGHHHHFHHGHHGHHHHHGGGGEHHHHFHHFF5FHHFEGHHHGDHGGHGHGFGGGEHFGHHGGGGGHGHHHGHHFHHB3FGHHFGGGG?GFFHCCEBGFFECCDFEGFCFGCHHGFDDHHHGHHCFGGGGGFBFDGFG?-:..AFG.-C0C009;:00;00:9/:CEFFF?AE::9;9?0:FEF0;0..--./00::B/090000000;A....
-@M01368:8:000000000-A3GHV:1:1114:16639:15258/2:R1:CCTAAATA:R2:TTATTATT:F1:GCCC:F2:ATGT
-CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCGCACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATG
-+
-GGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHGHHHHHHGGGGGHGIHHHIH5DEGHHHF?FGHGGHGGHEGGHFHHGHGEHHGGGGGFFFGHFBG2GHEBGHHGHGGEG/GFGABEDFGHEED?GGHHFFGGGCFEGD/GFHFFGEFGCGG?CC??D-EF@EEEFGCDDBBFGGGEBBFFF09090A.BFGA.9CCA0;EBAB00BBFF.@-./;BB;BFFF0:00099AAFFF0
-@M01368:8:000000000-A3GHV:1:1114:2404:13066/2:R1:TCCTACAA:R2:ATGGATCA:F1:GCAT:F2:CAGG
-CTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGACCATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACTGTCTCTTATACACATT
-+
-GGGGGGGGGHHHHHHHHHFFHHHHGGGGGHFFFHHFHHHHHHHHHHHHHHHGFEGGGHGEDFCDFHGHFG@@DGGHHHHHHGGGGCGGGGGEHGGCGBB?CF99EGFGGFGG?D9CFFFF/BBFFFFFEF9BFFAFFFFEFFFFFFFFFFFFFFFFFFFFF.FFBBFFFFFFFFFFFF-9;;;BFFFFFB9BFBFBFABFFEFFFFFFFFFF::BFFBFFFF.9//;FFFFF/BFFB/
diff --git a/tools/trim_galore/test-data/paired_collection_example_pair1_results3.fastq.gz b/tools/trim_galore/test-data/paired_collection_example_pair1_results3.fastq.gz
index ee71a9bc91..ff0dc41d36 100644
Binary files a/tools/trim_galore/test-data/paired_collection_example_pair1_results3.fastq.gz and b/tools/trim_galore/test-data/paired_collection_example_pair1_results3.fastq.gz differ
diff --git a/tools/trim_galore/test-data/paired_collection_example_pair1_results3.fastqsanger b/tools/trim_galore/test-data/paired_collection_example_pair1_results3.fastqsanger
deleted file mode 100644
index 0d55c14fd4..0000000000
--- a/tools/trim_galore/test-data/paired_collection_example_pair1_results3.fastqsanger
+++ /dev/null
@@ -1,392 +0,0 @@
-@M01368:8:000000000-A3GHV:1:1101:14518:9998/1
-GTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTG
-+
-AAAAAFFFFFFFGGGGGGGGGGHGGHHHHGHHHHHHHGCGHHHHHHHHHHHHHHHGGGGGHHHHHHHHHGHHGFHFE5BGEEHFGGGHHHHHHHHFBHHGGGGFHGHHFGHHHHGHHHHHHGEGGGGFHFHGEGHHGGCDGDGHGGGDGGHGGCGGGHGHHH/ACDG?.1FGCDCCGCA.CC@CDCHFHGFFGGGEBFGAB//CEFBFGG.:;D;;A0AFFFFFB..:@ABFF//;BFFFFFBF/9D:A
-@M01368:8:000000000-A3GHV:1:1101:18422:19051/1
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTA
-+
-CCCCCFDDDDDFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHFHHHHGGGGHHHHHHHHHGHHHHHHHHHHHHGGHGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHGCGGGHHHHHHHHHHHHHHHHHHHHHHGFDHGFHCFGGGGFGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF;FFFFFFFFFFFFFFFFFFFFFFFFFFFFEFBFFFFFFFFFF:FF
-@M01368:8:000000000-A3GHV:1:1101:25545:21098/1
-ATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATAAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGGCTTATTTAAGGGGAACGGGTGG
-+
-33AA?DFD5BDFGGGFEBDGEGHEGHGEGHCEGGHHCHGHHFFHHGFGAGE53FF2FAFFGDE5FFFE5GFBFGAEE1GHHHGHHHEHE3FGHF@GEGEGGHHGG3FAGFFDE?EEE3GFEGFGFGGCG?GHHHFHGGGC@DHFFHD/AA@AAAAD2ADDFFGGGGGFGGHA?EAEFBEAGHFABFGG5FDF5DB1EEGAFDFB53FF5FH@G5FFEHGHEFHFFHBE333GF43GCGGGGE@0?BFGGB0B?FHGFDGGHHHBFFDEGGHGFFFDFE@<1>@FFFGHHHHFHEFGDABFFGG/@DCE;>=EF?AEFFFF;9;=DAFFFFBBFFFBFFF//;BFB.:FBFFBB/BFFFF/BFFE?EFAFEF9
-@M01368:8:000000000-A3GHV:1:1104:11928:24197/1
-ACGTAGGTGCGATAAATAATGGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATA
-+
-CDDCCFCFFFBCGGGGGGGGGGHHHGHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHGGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHGHHHHGHHGHHHHGGHGEEGGGGFHHFHHHGHGEHHHHHHHHHHHHHHHHHGGGGGGFGGGGGGGGFFGGGFGFGAEFEFGFEAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF@D9
-@M01368:8:000000000-A3GHV:1:1104:12288:20635/1
-CACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGCCGCAGCATCTGTCTTTGATTCCTGC
-+
-ABA?AFBAB2>AFGGGGFFGD5GGHGFFHGHEGGGGHHHHFFHH5FHGHHHFFHEFHHHHHHGGHHHHHGFHGGHGGGGFHGGGFBHHHFGEGHHHHGHGGGGECEHHFHHGHHFFHFGGGHHHEGHDGHHGHGHFHHHECGCBCHHGFHHFHD1GFHEFHEF?GDCFFEFE0A@;@>BD/9BFBBBBFDFFFF99D=?>DDBFF-AFFEBB9BBEFEFAFFF//-@---;;FBBFBFB///;99999B
-@M01368:8:000000000-A3GHV:1:1104:13832:4398/1
-CACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTC
-+
-AAABABCAFFFFGGGGGGGGGGHFHHHHHHHHHGFFHHGGHGGGGGHHHHHHHHHHFHHHHGHHHHHGGHGHGGHGGGGGGGHGFFGGGGDGGHHHHGGGGFGGGGGCFGGEGCDGGGGGGGGGGGGEGF?;BFFFFFF
-@M01368:8:000000000-A3GHV:1:1104:23670:4161/1
-ATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATC
-+
-AAAABFFFFFFFGGGGGGGCGBBGGHHHHHHHHHGHHHHHHHHHHFGGHHHHHHGGFHHHHHHHGGGGGFHHHGHHHHHGHHHHGHHGGGHGGGGHHHHHGHHHHHHEGGGFHHHGFFDGEGHGHFHGHHGEGGGHHBGHGGHGHGGGGG/@FCDFDGFHGGGDFFHFGFFGGGGGHHHHGGGGGGGEGCDG?GGBFFFEE00BFFGABBBAFGGGEGEA.9FFF/./;BFBF./AAF/BFFBBF/F/BF
-@M01368:8:000000000-A3GHV:1:1104:24927:22253/1
-AGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTAT
-+
-AAA?AFFF3DFFFGGBGFF3EAEEEHFHHFHHHGHHHHDGHHGAEFGHHHGFCHHGHHHBGHHHAEGGHGGFHFBFHHHHHHHHHHFHH1EEHEEHH1FFDEEFCGHBGHHFHEHHHHHH1FCGHHHHEEFGEEGFFCHHH11>GHHF/FG2DGGCGAGGHGGGG@@@BEFFF?@@@FFEFFFFFF?@@FB@BB@FFFE=;==---A9EFBBB/;E;A9B/B/FFFEF/B///;/;9/A/;/9BFFFBFBB
-@M01368:8:000000000-A3GHV:1:1104:4705:7727/1
-CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG
-+
-BBBBBFFBFFFFGGGGGGGGGGHHHGHHHGHHHHH6GHHHHFFGGGHHHHHHHHHHHHHHHGGFGGGEGGGHHHHHHHHHHGHE3FGGGGHHHHHFHHHHHHHHHHHHGGGGGHHFHFCGFHHGGGGGGGHHGGGGHHGGGGGGHHHHGGGADCHHHHGDGGGGGGGGC@DGFHEHHHHHHGHHHHGFGDEGFGFFGGGGCFGBEGFGGGFG;C.BFFFFFFFBFBFFFF/F?.AFFF/BFFEFFFFFEFF
-@M01368:8:000000000-A3GHV:1:1104:7756:4776/1
-GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTG
-+
-CCCCCCFFFFFFGGGGGGGEEFHHHHGHGHHHHHHGGHHHGGGGGGHHGHHHHHGHHGHHHGGFHHHHHHHHGHHHFHHGHHGFGGGGHHHHHHHHGHGHFFHFHHHHHHGGHGFGFHHHGHG?FGHHHHHHHHHHHHHHGHGHHGHGGCDGFFHHHHGHHHHHGHHHGFHGHFHGHGHGH/@CABFFFFFFFACAFF?BBFFBFD-@-.D>FFAF.F.:9---;AADFFFFFBEFFF:9FFFFBBFFFF
-@M01368:8:000000000-A3GHV:1:1105:11310:21761/1
-ATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTT
-+
-CCCCBFFFFFFFGGGGGGGGGGGHHHHHHHHHHHHGHHHHHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHGHHHGHHHGHGHHGHHHHGGHGGG=FFGEHHHHHGHFGGHHGHHHHHFHHHHHHHGGHHHHHHHGHEHHGHHHHHGGGGF9AGE?DEGFFGGGGGGGGGGGGGGFFFF/9FFBFFFFFFFEFFFBFFFFFFFFFFFFEFFF
-@M01368:8:000000000-A3GHV:1:1105:11578:14676/1
-ATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGT
-+
-CDDDDFFFBFFCGGGGGGGGGGHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHFGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHHHHHHHHHGHHHHGGGGGGHGGHGHHHHHHH;CHHHHHHHHHHHHHHHGHGGGGFGGGFGGGGGGGGGGGGFGGGEGGFFFFFFFFFFFFFFFFFFFFFFFFF/FFFFFFFFFFFFFFFF
-@M01368:8:000000000-A3GHV:1:1105:13575:19255/1
-TTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGTTCCCGTGAGTGGTTAATAGGGTGATAGGCCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG
-+
-AA1A1DFFFFFBGG3A111FFAEGDDBGFDHF00F01BBA00BFGEHFBFFA1FABB0FAE?CFHF1BGFHFB0011F121E//>>1F10>B?EFHGCC/?/BFG/EEGFFEE//BCFDE0?E@@B/?11?//<@?/F<<AAFFFFFEAGGGFGGAA6FHDGGCGFHFFHFHFHHHHHGDDDFFFHGFFHGBEGEGHHHFFFHGEEAEGHHHHHFHHHFHGHFEDGGEGBEAGEFHHFFHHHHHHGGHHHGHHGBGHHFHHFGHHHHG?CG?AFHH1FHHHGHFHHHF?DGHFFHCGAGEFHGCBBBB?BFFF?B-B.9/BFFFFFB=-AD?..@FFFF@@-@@?AFD.FFFBBEEBD9/:FFFFFBB/:FF9BF/BF.BBFF/
-@M01368:8:000000000-A3GHV:1:1105:21099:2895/1
-TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATT
-+
-CBCBBFFFFFFFGGGGGGGGGGGGGFHHHHFHGHHHHHHFHHHHFGFHHHHHHGHHHHHHGHHEHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHHHHHHHFHHHHGHGHHHGGHHHHHGGHFFGHGCGGGHGHHGHHHFGHHFHH/FGGGHFGFEGGCFCCD;B@DFFFFFFFFFFFFFFFFFFFFFFFFF;F.BEFADFFFF.AEFFFFFFE;D.;:B00099:09BFBFFBFF.0:000BFFBF
-@M01368:8:000000000-A3GHV:1:1105:26469:19245/1
-TTATGATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCAAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATG
-+
-BAAAAFFFBFFFGGGGGGFFGGHHHHHHHHHHHHGGHHGHHHGGHHHHHHHHHHHDEGGHGHHHHHGEDHFHGFHGGHHGGHGGEHHHGHHGHHGHHHGGEGGGHGHGHHHHHHGHHHHGHGGGGGHFHGGGGHHGGGGCDGHHHGD>A@FFFFFFFGGGFEECFGGFFB5GBHH5DFH5GBEEAEFHFHHF3F3GHFHHHEF3GAEHBGFHFFEFFHHHHFFB31FGGEGFHGEHHHHHHHHHHHHHHHHHEEEEGHHHFFF3GF3FGF44BBGEHHHHGG2B/BGGHE;B=.9.;.BBFFF..;A;/B/;B//;;//BBF
-@M01368:8:000000000-A3GHV:1:1106:22927:3863/1
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGACTT
-+
-A>ABAFA?D@AFGGGGFGGGFGFHHHFFH4BCBGHHHHGFFF3G5BF3322EEAFHHHHHHAB5GHFHEHFHHH3BEEHFGF5@13FDF3GDGFHHFGHHHGHHHGFGGHHFCG//?/EGHHHG3F4BFFGHHHEDGFHHB/B/?FBF<@-9?BDEFFF?B--?DFFFB9FA>B;A.BFDB?AB.--@;-.FFAEFFFF9AE;DDFFFFB/9BF
-@M01368:8:000000000-A3GHV:1:1106:23625:4236/1
-TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATT
-+
-@?AAAFFBFBFFFGGFGFGGGEFCGGHHHHFGHGHFHGHHHHGGFGGFGHHGHGHHHHGHGFFHHGGGGGHHHHHHHHHBFHHEFHHHHHHGF?FAGHGFHHHGDGFHHGGGHGHFHHHGHHHGHHFHHGFFGFHHHGHHHHFFHHHHH/FGHHHHGHGHGBFFF9
-@M01368:8:000000000-A3GHV:1:1107:14451:26748/1
-GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGAC
-+
-BABBBFFDABBBGGGGGGGGGGHGHHHHGAGHGGGGGHHHHHHHHGHHHHHGGGGGGFFHHHHHHHHHFHHHHHGGGGCHFHH3FEHHGCGGGDGHHHGFGGHHGGGGHHHHHHG/DGGGHHHHGGGGGCCHGG?CGG1GHHGHHHHBGHHHFHHGHHHHGGADAD00ECEGHEFHHFFAEFFFFFGGFBFBFFFGFFG.;B/99;BCFGGGGEFFFFFFEDA./:ADEB?FBFFFF;FFEBFBFF
-@M01368:8:000000000-A3GHV:1:1107:18714:24788/1
-GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACC
-+
-BBBCCFFFDEDEGGGGGGGGGGHHHHHHGGHHGGHGGHHHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHCFGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHGHHHHHHHHHHHGHHHHGGEGEGCCEGGHHHHHHG:CAFFGGGGGGBFEFGGGGHGAFFGGFGGGGGGGEFFFFFFFFFEF/EDDFFFFFFFFFBFFFFFBBFF
-@M01368:8:000000000-A3GHV:1:1107:21355:8333/1
-ATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATG
-+
-ABCBBFFFFFAFGGGGGGGGGGHHHHHHHHFHHHHHGGGGGHHHHHHFHHHGHHHHHHHGGGGGHHHHFFGHGHGGGGGGGHGGEGGGHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHGHHHFHHHHHHHHGHFFHHHHGGHGEGHHGHGFHHFHGHCEFHEGHGHHHGHHHGGGGGGCFFFFBFGGFGGGGFBG?FDGGGEGFFFFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFF.AA/9
-@M01368:8:000000000-A3GHV:1:1107:22864:15712/1
-AATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTAC
-+
-BBBBBFBBFFFFGGGGCEGGGGHGHHDGFHHHHHHHHHHHHHCHHGHFGBGDFGE?GHHGHFBHHFHHHHHHHHHGHFGFGHGHHGGC@DCFGHHGGGGGGGHHHHHHGGGGGFGGGGFFGGGGFADGFEFFFFFFFFFFF9;FFFF/BFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFEAECDFFFFFFFFFBFFFFF
-@M01368:8:000000000-A3GHV:1:1107:24817:14233/1
-CTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGA
-+
-3AABABFFBFFFGCFGGGGGGGHHHFGCFGGGFFGHHCGHHHGHGHGHBFFHHHEDGGFGFGHHFFFHGGEGHFHHGHFHGFFHHHFGHHHFHACECEHHHBGFHGFHHHFHHF4GFHFHFHEHGGE/EG:A-CFGGFGADGGGGFGGGGDAAAADFA.-AFFECFDAAFFFFEFFFFBFF;D;BFFFB/;BFFFFFFFFFFFFFFBFFBF/;//;BFFFF?FFFFFFEDAFFFFFBF//:BFBACF
-@M01368:8:000000000-A3GHV:1:1107:24830:14440/1
-AACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCA
-+
-ABBBA@FFFFBBGGGGGGGGGGHGHHGHHGHHHGGHHHHHGHGHHGDHHHHHHAGGGGFHHGHHHHHHGHHHHHHHHHHHEGAFGFHFHHHHGEGHHHHHHHHHHHHHHHHHGGHHHGEFEGGHHGHHHHHGGHGFHH/?GHHHHGFGHGFHCGGBBDAFFFEFFFFFFFFFFFFFF=CDDADDFFFFEF=BFFFEFFFBFFFFEEBF./:FFFFFFFFFFFFFFFFFFFEFFFFFFFFFFF/BFBEFF
-@M01368:8:000000000-A3GHV:1:1107:26478:23602/1
-GCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGAT
-+
-1AAAAF1B13BBBEGGGFGGGGGGHHDB1BGFHHHFGFGGG0F00BGBGFGGF1GBGB1BGCGAEE/FGFHGHFHHHHHBFF1B0FGGF//F//FGFGFFF/1@B1DFHHHGGEFEDHFDFAFF0B//1FDD1GFFHHDF?1BD>GF/GCAC0C@?@-;99BBB->@--;-B//99B--9-BF=A--A-:A----9AA9AFBFBBB?E@BBFFFFFFFBFFFFF
-@M01368:8:000000000-A3GHV:1:1108:11059:23181/1
-GTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGA
-+
-CCCCCFFFFFFFGGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHGHHGGGGHIIIHHHHHHHGHHHHGHHHGGHGGHHHHHHHHHHHHHHGGGGGHHGHHHHHHHHHHHHHHHGGGGGHHHHHFGHHHGGGGGGHHHGGGGGHGGGGHHHHHHGGGGGGHGGHEFGGGGGHGGG-ECHHHGGGGGGGGGFGFFGGGGFGGGCF?EFGG0;BFFFFB?9AAFFFFFFBF;BBFFBFFFFE?F
-@M01368:8:000000000-A3GHV:1:1108:12233:13498/1
-TAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACC
-+
-BCCBAFFBCCCCGGGGGGGGGGHHHHGGGGGHHHHHHGHGHBGFHHHHHGGGGGHHGGBFGHHGGGGGGGHHHGGGGHHGGGFHHHHHHGGGGGGHGHHGGGGGGGGGGGGGGHHHHFHHHGHGHHHHHHGHGHHGGFGGGDFFGGHHHGHGH/FHHHFHGHGHHGHHHGHHBAAAA3BDFFFFDGBFFEEGGGGHHGHFHHCFHGHGHHHGF3BFGFHGFEFHFHGH3EGFC1EEGBFGBHFGGFFHHHGHFHGHHGHGGHCEHEBCFF3ED3@GH4FDEG12FFHFFHG/G3FGA/@EFEFCG33FFGBBGHFHH/?BFBGFGFAGC2C/@-;@-;BFFEFADF?DFFFFFF?;AFFA;AD;..FEFFA--;9;D.9BFFBE?;.;///://///99//BB/9;F./B//BBFB9//
-@M01368:8:000000000-A3GHV:1:1109:25237:11722/1
-CCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATG
-+
-AABBBFFFFFBBF5BGGFGABGHGGAEFGFHHHHFHCHFHHBFGGGEHBGGHFFFFGFAEF5FHH?EGGF5AEG3?EBFHD?C/EFHFFEGEE?CFGGGHHFHB3EEFG?EEGHCFGGGGGGGECA?FFFDFBFGCG4EGFG??GACFGGHFHCFHCHBECDEFCEGHHHHHFFGGF3AFFHHFGEGCGHHGEHGFGHHEGG?EFHHH?EGGHHGGGGHHHHHHEGGGGGHFHHGFDDGGACFCC@?/FDDGHHHGHEFFHHH0FDHHFFEDCD.CFHHGDFGGGGGEFGC?EGHHHB0CGHHGCGHHHHBEHGHH.::ACA;FECFGGGGGFFFEFGGGGGGGFFBBBFGBFFFEFFFFBFFFFFFBBFBFFB9..AF?FFFFF
-@M01368:8:000000000-A3GHV:1:1110:14751:26540/1
-TAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACTGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGTTGTGCACGCGATAGCATTGCGCGACGCTGGCGCCGGCGCACCCTATGTCGCAGTATCTGTCTTTGATT
-+
-AAA?1CFFFFADGEEGFGB1112DGDGHGFGHHCG/ECEBGGHDFGHHFHGHEB1BCGFFE2EGHHGHFFFHHBFFHEFCGGHHFHHHHH111BFFHGHEHBEHGGECEFHGFHFGGDBBGHHBHHHH0GH22FGHGFFFH1BFCG0F@FGG11@GDHFG1D/1FGFHH/FCG.>0><-<@CCFFG.CC-@C?C9FFF0C---;------;9----9-9@-AAFF9B9---9-/;B9BF/9BB//B/
-@M01368:8:000000000-A3GHV:1:1110:15519:10008/1
-CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAAC
-+
-BBBBBFFFFFFFGGGGGGCBGGE?GGGHHHHHGHHBEGGHHGGGGGHHHHHHHHHHHGGHHHHGFGGGGHGHGCFGHHHGGGCGGHGHGGGGGHFGGGHHHHHHGCEFGGHHHHGGGGGGGHGG/CEGHHHHGFHHHGFHHGFFHGHGHFGGEGFDDGGBCGHHGFHH/CHHHHGHGHF0CFGGGGGGGFFFFFBFFFFFFGFFGGF?DGEDEEFEFFFF?FFFFFFFFFFFFFFFBFFF.BFFFBFBBA
-@M01368:8:000000000-A3GHV:1:1110:16559:7507/1
-CATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAACTGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGAT
-+
-ABBBAFFFFFFFFGGGGGGGFGGGGAGHHGCHHGHHHHHHHHHHHGHHGHHHHHHHHHHHHHHHHFGGGHHHHHHHHBDHHHHHGGHFEEGHGGHHHHHHHGHHHEHFGGGGGGHHHF33FGHHG3GDDGH//EEGHHFFBF?GHHFCC@DGFCGGB?DGHAFGGHHGFH0E??<@DGHGGCFGGF?DGGGFFGGGGGGGG.C/0BBF?FBBEGG0FFF.AA9;B9/E////99;;:.//:FFFFBBF//
-@M01368:8:000000000-A3GHV:1:1110:16700:6843/1
-AAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAAC
-+
-BBBBBFFFFFFFGGGGGGGGGGHHHHGHHHHHHHHHHHHGGGGGHHHHHGGHGHGGGGGGHHHGFFGHHGGGGHHHHHHCGG@FGHFHFEGGGGGGHGGGGDGHHHHHHHHHHHHHHGHHGHGHHGGGGGCGHEHGHHHFHHHFF.DGHHHHHHGHHHHHHGFGHGCGHGHHHHHHHHGHHHCD?:A::FCDGEGFGFFBFGBFGGGF00CBFFFF/BFBFFFFA
-@M01368:8:000000000-A3GHV:1:1110:18014:7856/1
-ATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATG
-+
-AABBBFFFFFFFCGGGCGGGGGGHHHHHGFHHHHHHGHHHGGGGGFHHGHFGGHHHGGFGGGHHGGGGGHHGGGGHGGHHHGGGGGGHHGHGDGGGGGGGGB/ACC=@--AFBFFB?=BFDFFF//FDB;-9A-AD-.A.A=?-9-:B/AEB/BFF.A;DDFFFBBFFFF/BBFFFFBFF9B
-@M01368:8:000000000-A3GHV:1:1111:13195:14562/1
-ATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATAC
-+
-ABAAAFFFFFFFGGGGGGGGGGGHHGG4FDFGFGGCGGGHGHG?EGHEGFFDHFHHHHGGFE@EHHGEEEGFCCEFFGFG/EHHHHHBFBGGHFHFHEHGHEFGGGED/EFDEFFEHHBGHHCFFFDFGGHHGFFFHHHHGFG>0<0DGGFHBGHFBDFGHGFFGGAGHHGCGBHFFHHHFFFBFGGGGF/:FFFCB9F9FF0FEFE?FF0BED-D./
-@M01368:8:000000000-A3GHV:1:1111:14287:20817/1
-GCCAACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTCATCGCACCTACGTTCACTATTACAGGCGAACATACTTATTAAAGTGTGTTAATTAAT
-+
->>A3>AAAACFFFGFGFGGGGGHGHHHHHHHGGGGGHGHFFCGHHGBGHFHHHHAGHHHHEHHAGFFEHEAE?GHHHHEGFFFDGHGHBFFHFHHHGHGHGHBECG@@@EHGGEGCGGGGFFGGGG?BGGGGGG.-C.FEDCFFFEFFFAF/99BEFA.:9BFB/9FFFEFFFFFFEFFF/FBBFFFFFFFFFFFB//;.@@//;DFFFF//BBFFFBF;A;DF=>B//;9B//B/;/;9//9//;B/:B9
-@M01368:8:000000000-A3GHV:1:1111:23624:14516/1
-GTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGGGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTA
-+
-3>3AAFFFFFAFGGGGGGGGBGHHHHHHGGHGGHEHGHADFHHHHEGGGHBGHFFHHEHHGHFHGG5FBEEGEGHHHGHHHEFDGHHFFGFGGHHGHHHGHBHGHFHFHHGGGGFCFBGFGE?GFBECEGDGHEHGDGCGHGC/DF@GHGFFCGGGGFFFFDD?@C@GFEC?-ADHFFHBGHFBHFG0CC//.;B0/CA.;AA.;@.AB/9BB/A/.AFFFE/BFFF///9B/FBDE9/;99/B/BFFEF
-@M01368:8:000000000-A3GHV:1:1111:7437:24508/1
-ACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGT
-+
-CBCCDCFFFFFFGGGGGGGGGGGGGGHHHHHHHHHHHHHHHHGGGFFEHGGHHHHHHHHHHHGHHGGGGGHHHHHHFHHHHGHHHHGHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHGHHGGGGGGGHGGGGGCGHHHHGHHGHHHHHHHEDHHGHGGGGGD;BEGGGFFGGGGC:CFGGGGGGGGGFGGGFFFGFFFFF;
-@M01368:8:000000000-A3GHV:1:1111:8665:25542/1
-TCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTGTCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGG
-+
-AA3AAFFFFFFFGGGFGFFGGGGHGH4AGGGFHHCGEE2FGHHHHHHHHHHGFHGF53FHEGGFAF5CFHHGGFGHFHHGGHHHCHHHHHHHFHHGH?EEEG1FFFEHHFHFGGHHGG?F4FBGHGHCHEF0//-9--;0CFGG?@BGDGFFFFEFBBBDA?FFBFFFF=B;-9.AA9EFFFF/;9BD.//;9/9/B:/:BFBFFBFFF/B:BFF//;/B/FFFBFF.A-BFFFBA/F//BFFFBF/;/
-@M01368:8:000000000-A3GHV:1:1111:9655:13916/1
-GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGCGGTGTCCACGCAATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTT
-+
-1>>1AAFFFFFBGGFE1EEEEGGHGHHH2FGHHHFGFGHGCFGGGEFHGGEGGHHHHGHEFBFEGHHHGHHBEHHHHGHGFHHHGGGGHHEGGGGHHHGFHGHHHH1FHHG?G/AGF1GBFGEFFHF2FDD0FG--99@=---AAABA-BF/B?A@F-9BBFF//BB
-@M01368:8:000000000-A3GHV:1:1112:10272:7828/1
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCAC
-+
-AAABABBBBBBFGGGGGGGGGGHGHHGHGHHHHHHHHHGHHHGGHDGHHHGGGGHHHHHHHHHHHHHHHFHGHHHHGGHGGHHHFHHHHHCGHFFHHHHHHHHHHHHHGHGEFGAEEEEHHGHHGHGHHHHHHHGHHHHHHGHGHFFBC/BA?BGGGFFFFFFFFFFFFFFFC-@DDFFFFFFFEFAFFFFF
-@M01368:8:000000000-A3GHV:1:1112:9832:16531/1
-CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGGGCTGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCTTGAAGTAGG
-+
-AABABFFFFFFFF4GGGGEBGEFGHGGHGHGHHHGGHFBGFGGGGGHHEFHGEHFHFHEHHHHHGCGGGHFHHCFGHE@1EEEFFGB?EEEEHBGGGCHHGHHBEE@>EAHHFAEBDGGGCEFF?CEGFFHHHHHFHHHFHGHCGCAHHF<..C.D=0CD.GFHHHG.CAGHC:CHB0GGGFFB0:CFA.A-;9.;000CBBFFBFGA.9AA;A;EDDE.ABBFFFF9;BEFFF/BF//9/B//9:B
-@M01368:8:000000000-A3GHV:1:1112:9832:6701/1
-CGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGG
-+
-AABBBBFFFFFFGGGFGGGGGGHHHGGHGGHHHHHHHHHHHGHHGGGGGHHHHHHHHHHHHHHHHHHGGCGDHHHHFGGHHHGGGGGFGGHGGGGHHGGGGHHHHHHGGGGGGHHGGGGDGGFGHGGFCDCHHHHGHHHHGHHFAGFGGEHFGEG.C1>11F1D1DDFFFFGGG1FH3GHGHGHGHFHEC?EC0B1FBGFB1GHHH21AF2HH2FEGEHAE10AAE/>/FFHE?/E/?E1@EFFH?E@/EGGG//B11B//<@@C0F/<0AAAAFFFBFFFGGGGGGGGGGHHFHGHHHHGHHHHHHHHHHHGGGGHHHHFHHHHFHGHHHHGAFFEFHEGHHHHHHHHGHEHHGGFGGGHHHHHHFHHHHHGGHHHHGGGGGHFHHFF?HHGGFECEFFGHFFGFHGECDGHGBGFHGDF@@?CGFHCEGGGFD.CCC?EGHBHHHFHHFBCFFGEB/CEGGGGDAA.90C9CEBFGGBBF/9.9FBFFFBBFF//99FFFFEABF//99FFEFFFBFF
-@M01368:8:000000000-A3GHV:1:1113:5741:16959/1
-TAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTAT
-+
-ABBBBFFFFFFFGGGFGGGGGGHHHGHHGGHBGHGAGFFCAFGHGFFGHHGFHHHHHGGGGGHGHHHHHHHHE3BFFHHHGG0EDF@GHFFGGGHGGGGGGGGGGGGGHHGGEEFHGFHHDDG@DGGGHHGDGGGGGHGG?CF?HHGHHHGHGHHHFFHGGGHHHHGGCD.;0A33>FFFFFF@FFFGGGGFGGD4EGGGGGHHGFFGHFGGHHHFEFHECFHHHEHGAGBCCGCCEGGHGHEGFBFHFHHHHGGGHFHGHEGGGFEGEGG??@DBGHGGC-@FFGHFHFHB-??DA-DD@9:BEBG@B?E?A.ABBFBFA??F.FF/BFFB/B9B/9
-@M01368:8:000000000-A3GHV:1:1114:14540:5315/1
-CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGGGGGCTATTTAGGTTTT
-+
-AABCCFFFFFFFGGGGGGGGGGHHHHHHHFHHHHGHHGHHGGGHGGHHHHHHHGHHHHHHGGGGGHHFHHHFGHHGGFHHHHHGGGGGHHHGHGGHHHGGGGGGHGHGGGGHHGGGGHHHHHEGDDFGFFFHHGGGGGCDAFCFGFDHHHHGGHGHHHHHHBCGEHHHHGGHG.ACGEHGG0CBFFF:A;BB0;09CGF00CFFFE0AA?//CFFFFFFFFFFFFFFFBEF;A.-=A--:BBFB90;;FE
-@M01368:8:000000000-A3GHV:1:1114:15066:16302/1
-TAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAACGGTTGTTAATTAATTATT
-+
-BBBBAFFBDFBFBGGGGGFGGGBGFFFHGFHHGFFFHGHHHGHHHHFFHHHGHGC?AEFFHEFBFFFGHHHHH1FDGFFHGHGHFEGCGC-<>ABFFFFFFGGCGC1BBF1GHHHBHHHHGFHGH1A100AA/GGEHGFBAA1FFD2FHHHHECG2F1BB/E0FC/A>EE/FGGFGEF1EGBBEHHCGGGHBGEHFHE0B?E--;C?CCGGG@A@GBFBBBB09?@??A-AB--ABE@=-=-9AE9A;FFFFFE=9-//;//;9FF/////;;///9B///;/B////9FFBB;--A@-;/9;9-:-/;;FFFE9BF?@;-9-99/B9F/://///99/;
-@M01368:8:000000000-A3GHV:1:1114:2404:13066/1
-TCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCAT
-+
-CCCCCFFFFCFFGGGGGGGGGGHHHHHGHHHHHHHHHFFHHHHHGGGGHHHHHHHHFHHHHHHFGGHHGGHGGHHHHHHGHHFHHHHGGGGGGHHHHHHGHHHHHHHHHHGGGGGGGHH?FGHHHGGGGGGHHGGFGGHHGGGGHHHHHFGGGGFGHGHHGGGGGGGHGGGEGGHHGHHHHHHHHHGFBFFDA0FGGGFFGG0:EFGGGGGGGG;AEBF0B0BFFBFFFFFFF
diff --git a/tools/trim_galore/test-data/paired_collection_example_pair2_results3.fastq.gz b/tools/trim_galore/test-data/paired_collection_example_pair2_results3.fastq.gz
index 176d5feaef..6f0f7d8782 100644
Binary files a/tools/trim_galore/test-data/paired_collection_example_pair2_results3.fastq.gz and b/tools/trim_galore/test-data/paired_collection_example_pair2_results3.fastq.gz differ
diff --git a/tools/trim_galore/test-data/paired_collection_example_pair2_results3.fastqsanger b/tools/trim_galore/test-data/paired_collection_example_pair2_results3.fastqsanger
deleted file mode 100644
index 2e31e60478..0000000000
--- a/tools/trim_galore/test-data/paired_collection_example_pair2_results3.fastqsanger
+++ /dev/null
@@ -1,392 +0,0 @@
-@M01368:8:000000000-A3GHV:1:1101:14518:9998/2
-CATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATA
-+
-CCCCCFCCCCCFGGGGGGGGGGHHHHHHHHHHHHHHHHGFHHHHGGGGGHGFHHHHHHHHHHHHHHHHHHHGHGGEHGGGGCGGGHHGGCGGGGGHHGHHHGGGGGGGG.BFFFGAGADFGAFDGFGGCFFF;DDFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFBFFFFFFFFFFFFFFFFFFF09FFFE00;BE@;DABBFFFFFBBFB00;F:9;FFBFFF9BFFFFFFFFFFFFF90/::BFFFBF
-@M01368:8:000000000-A3GHV:1:1101:18422:19051/2
-CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG
-+
-BCCCCFFCFFFFGGGGGGGGGGHHHGHHHHHHHHHHHHHHHHGGGGHHHHHHHHEHHHHHHHGGHHGGHGGHHHHHHHGHGGHHHGGGGGHGHHHHGGGHFHFHHHHHGGGGGHBFFCGDHHHGGGGGGHGGGGGGHHGCGGGFGHHBGGGGGFFFHEGGGGGCDCCE@EFGHHHHFHEGHGFFHHGB;ECBFGGGEFEFFGF0AFGFGFFG.;;DFFFFFFFFFF090BFFFE?FEFBBFBFFFB990BF
-@M01368:8:000000000-A3GHV:1:1101:25545:21098/2
-GCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTTCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCAACCTATGTCGCAGTATCTGTCTTTGAT
-+
-3AAA34BFFFFFGCGGCGGGCGHHHGHHHHCGHFGHFHGFGGCDGGBD5FGFFFHFHGGGHAEBFGHHHEHGGFHBGGFHHHGGGEEFHHHFHFFHDHGHGHHHHFFHHGGFAEGFFFFFBGHHHFFHHHHHHHFHFHHFHGFHGHGA/<@EFHEHH1GGHFFFHG1>=FGHFHGF.GE//-AADEBGGGGFFCGGHHA5DFGAE?AAEDFHG2AEE1FBE00A1BGE0?E1FFGEEEGB4F43?EE/>///F??//@BCBAC<<.<<AABBFFCFFFGGGFGGGGGGGGHHHHHHHHHHHHHHHHHHGHHHGGEGGFFHHHHHHGHGHGHHHHHEHFHGHHHHEFHHGHGGGGGHHHHGHHHHHHHHHGFGHHHHHGHGHGHHGGGCCGGGGFGGGGGGDGGGGFGDGGGFFFFFFEFFFFFFFBAF:9AF//;BEF-DD/FFFFBFFFFFFFFFEBFF?FB;/;//BFFEFFE/::..9;-:B9:FBFFFFFF
-@M01368:8:000000000-A3GHV:1:1102:17584:10050/2
-GTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGAT
-+
-ABCCCFFFFFCFGGGGGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHFHGGHGGHHHHHHHHHHHHHHGHHHGGHHHHHHHGGHHHHGGGGGHHHHHHHHHHHHHHGGHHHHHHGHGHGGHGGFGGGGGGGGGGFGGFGGGGGGGGGFFFFFFFFFFEFFCFAAFEFEFFFFFFFCFDFFFFFFEFFFFFF
-@M01368:8:000000000-A3GHV:1:1102:18842:24844/2
-CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG
-+
-CCCCBFFBFFFFGGGGGGGGGGHHHGHHHHHHHHHHHHHHHHGGGGHHHHHHHHFHHHHHGHGHHHGGHGGHHHHGHHHHHHGHHGGGGGHFHHHFGHHHHHHHHHHHGGGGGHHHGFECGGHGGGGGGHHHGGGGHHGGGGHHHHHEFDCADGHHHHGGGGFC?AE-@DDFHFFHHHCFHGGFFGFFFGGGEFEGFGED9C?CEGF90FEF/A./BBF9FFFFFFFFBFFF.9AF////;FFBFFB/:BF
-@M01368:8:000000000-A3GHV:1:1102:21788:11027/2
-AATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGC
-+
-CDDDDFFFFDDFGGGGGGGGGGHHHHHGGGGHHHHHHHHGHHHHHHHGGHHGEHGGHHHHHHHHHHHHHHGGGGEHHHHHHHHHHFFFFFHHHGGGGGHFHGBFEHHHGGGGGGGHHGGGGHGGGGGHHFHHHGGG@CGHHHGGGGGGGGGGGEGDGFFDHHGEHHBCFHGBGFAGGHFGGCEEF0BE///FDFACF?FHFGC//??1B0</@GFGD/@1FG10FF>DFCFDFFFFFFFFF-BBAFFFFFFFFE@D9B;FBFFFFF
-@M01368:8:000000000-A3GHV:1:1103:25465:20010/2
-AACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGTGCACCCTATGTC
-+
-AAABBFFFFFDFGGGGGGGGGGHHHHHHGHHHGFHHHHHHHGGGGGHHHHHGHHHHHHHHEHHHHHGHGGHHGGGHHHHGGGGGHHHHHEHFGEHHHEE14BGHHHGHGHFCE/EFGGGGGGHGGGGGG?FFGGGG.ADGGGFFDA.FFEC--;9BBBD?FFFFF
-@M01368:8:000000000-A3GHV:1:1103:25519:6301/2
-CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATGCTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGGGATGTCTTATTT
-+
->AABBFFFFFFFFFFGGGCAFGCGHGGGHGGGHHFHHHFHHGGGGGFFFHHHBHHHHG5BG5GFEEEE1FGFFGF1EGHGGG/BFHEF@EA1BE1@EED3?4DHGGGGEGHFFFDDG?DCDEG@<?@DGCAGC-;@DGFFHGCHEEFGGFGFB/;..BBB.CCE?A0FE0EBFF0FEF.;=DFFB//BFBF//;/:9BA.9FB9//;9//;9F;FF
-@M01368:8:000000000-A3GHV:1:1104:13832:4398/2
-GACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTG
-+
-BBCCCFFFFFFFGGGGGGGGGGGGGHGGGGGGGHHHGFGGHHFGGGHGHHGHGFGECGHHHHGGGGGGGHEGFGGGHHGHFHHHHFGHFHHHHGHHHHFGGFGG3FFGGHHHHHHH2?FFGHFHHHHHHHHHBGHDGC0
-@M01368:8:000000000-A3GHV:1:1104:23670:4161/2
-CGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTT
-+
-A3AABBFFFFFFGGGGGGGGGGHHFHHHHHHGHGHHHHHGCHHHHGGGGHHHHHHHHHHHHHHHGHHGHGHGGHGGHGHGGHHGFGHHGHHHEHHGHHHHHGHEHEHHHGGFGGHHHFHHHGHHHHHHHGHHHHG4EGGGFCBBCA?DGGGGGGCCAFFFFFFFFFFFFFFFFFDFFF.9@--=AFFFF;FBFFBEFFF9FBFFB9/FFFFFFB/FFFFFFFBFFFF/BBFBB/FFFE?.;@FFB?FB
-@M01368:8:000000000-A3GHV:1:1104:24927:22253/2
-GTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGGGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGGGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAA
-+
-AABBBFFFFFFFGAFGG5GGGGHDGHHHHHHHHHHHHFHCFGC2F3BFGFGHBGHHFHEDHFGGGGGHHHG3F?GHHGGC/EFEHFGAEFFHGGAEHGGGG3EEGEEDHFHAAD@DGGGHG/AC1>GED-<<-.;C0:0C:C;GH:::::0B90;FFGEGFBFF0C.;9F0;0090;900;00FA9D.A...-9=BBFFFE/FE9;9E//BBBB.BBB///B/9/B
-@M01368:8:000000000-A3GHV:1:1104:4705:7727/2
-TCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCG
-+
-ABBBAFFFFFFFGGFGGFGGGGGHHHHFHHHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHEDEFGHFHHHHGHGHHHHHHHGHHHHHHHHFHGHHHHHGGGGGHHHGHHHHHHHHHHHGHHHHFHGHGHGGHGGGDCD.;FFGGGGGGGGFFFFFFFFFFFFF.>FFFFFF-BBEFFFFFBFFFFF-DDFFFFFFEFFFFFFFFFFFF?F///9FFFFF/;FFFFF?AADCFFEFAFFEFFFFFB/;B/A9
-@M01368:8:000000000-A3GHV:1:1104:7756:4776/2
-CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG
-+
-BBBBBFFBFFFFGGGGGGGGGFFHHGHHGHHGGHGHHGFHHGGGGGHHHHHHHHGFF3GGGHGGGHFDHEGHHFHHHHGFHBCGHGEGFFFFFHHHHHGHHHHHGHHHGGGGGHHGHHGGHHHGGGGGGHHECCFDHHADGGHHHFGFDC////FHHHDGFCGCDEECF?EGGFFHHFEHFGGHHFFF/A900;C.9..C0990.;CFBFGE/9.9B99/;B.99/FB///9AEF/99/9/BBF/B/B//A
-@M01368:8:000000000-A3GHV:1:1105:11310:21761/2
-ACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCTC
-+
-BCCCCFCCCCCCGGGGGGGGGGHHHHHHHHGGGGGHHHHHHHHGHHHHHHHHHGHHHHHHHHGHHHHHHHHHHGGGGGHHHHHHHHHHGHHHHHHHHHHHGGHGGHHHHGHHHDGHHHHHHHHHHHHHHHHHGHHHHHGGGGGGHHHHHGHHHHHHHHFFHHHHHCCAHFHHGGFFFFFFFFFAA;BDDFFFF00.-=D.ABDA-.::AAF;F@.AAD./0000.9-;.9:00009:F090;FF000900
-@M01368:8:000000000-A3GHV:1:1105:11578:14676/2
-GCTCCGGGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTAT
-+
-CCBCCCBBCCCCGGGGGGGGGGHGGGGGHHHHHHHGHHHHGHHHHHFFFGGHGHHHHHHHHHHHHHHHHFGHHHHHHHGHHGHFHFGHHHGGGGHHHHHHHHHHHHHHFHHHHHHHGGHGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHDGGCGHHHHHHHHHHHHHFF>ABFDCFFFFFDGFGFDGDEG1EGEFFBFHFGHA0E00A0CAAC0FFDFFAGHFEHHFDH/EAFCFFHACGHHAFFEF2DFFG/FGHHHHGHGH0C@/E@GGC0FG10>GGFGHHGHFFBGFGB2>F@;-A@FF<-;---@-;-----;-BFF/;---9//BBFFFF//B;FF///9BF///;/;B/BBFBFFBB/;-9--///;9/:-;BFFBBB9F
-@M01368:8:000000000-A3GHV:1:1105:15502:11453/2
-GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGAC
-+
-CCCCCFFFCFFFGGGGGGGGGGHHHHHHGHHHHHGGGGGFHFHHHHHHGHHHHHGHHHHHHGHFHGHGGGGGGGGHHHGGGGGGGGHHHGHGGGGGGGGGGGHHGGGFGGGFEGGGGFFFGGFGDGGGBFGFFFFFFFFFFFFFE0BFFFFFFFFFFFFFFFFFFF;BFFDFFFFBF0BFFBFFEEBFFAA009FFFFFBFFEBB0;.BFFBFFFFFFFFFFFF0;BFFFF
-@M01368:8:000000000-A3GHV:1:1105:16787:9771/2
-TCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAG
-+
-CCCDCFFFFFFFGGGGGGGGGGGHHHHHHHHHHHGGGGHHHHHHHHHHHHHHFHHHHHHHGGFGGHHEHHHHHAGHHGHBHHHGHHHHHHGHGHGHHHGGGGGHHHHHHHHHHHHHHHFFHHHHHGHGHGHHGGGACAFGGGGGG-AFGGFFFFFFFFFFFFFFFFFFFFF-9@AFEDFFFFFB.9-AABBBFFFEFFFFFFFFBFFFFFFEBB0BBBFFBFFBFBEDEAFFEB.ABFBFBFFFF0::
-@M01368:8:000000000-A3GHV:1:1105:17564:15723/2
-ATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTT
-+
-CCCCCCCCCFFFGGGGGGGGGGHHHHGHHGHHHHHHGHHHHHHFHHHHGGGGHHGHFHHHHHHHFHHHHHHHGHGGHGGHGHHHHFHFHHGHHHHHHHHHHHHHEHGHHHHHGGGGGHHHHGEHHGHHHHHHGHFGHFHGGAHGGHGGGCCBDFGFFAFDBDDFFFFFBDA;AAD-ADC?AFEFB-@@AAEFEFFFFF..-:.B0:FFFFFFEF0BFF09FF.0;9BFFFFF0BFFFFFF?.::-909;.9
-@M01368:8:000000000-A3GHV:1:1105:18108:12091/2
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTA
-+
-CBBCCFCCCCCFGGGGGGGGGGHHGHHHHFHHHHHHFHGHHHHFHFHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHGHHHHGHFHGHHGHHHHHHGHHHHHHGHHHHHGGGGGHHHHHHHHGHHHHHGFGHHHHHGHDHGHHGCA;?AAFGFEFDFFF.FFFFEBDFFFFFFFFBFFFFFFFFFEFEFFFEFFFFFFF.:BFFF0BFFFFFFFFFFFF;0FF0;BFFFFF
-@M01368:8:000000000-A3GHV:1:1105:18331:16683/2
-TTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGGGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATAT
-+
-CCCCCFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHGHHHHHFHGHHGHHHHHGGFGHHIIHHIHHHHHHHFHCFGHEEHFFHGHHFGHGHGHFHHGGGGGGEEGHHEHFHHGGHFHHFGGGGDFGBCGG?DGHGGFCGGHHECEF?CHGGDGFFGGHHC?EEGGHHHH.>AEGGGHEE-AEG0C0GCCEFFFFGEFG/;CEGGGE.:CAE.;@.@EFBFFFF/..:BFF0:BB00FBFFFFBDAFFF0;
-@M01368:8:000000000-A3GHV:1:1105:20577:18421/2
-ATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGCCGAAAATACCAAATGCATGGAGAGCTCCCGTGAATGGGTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATT
-+
-?ABBBFFFFFFF4CFFGGGFGGHHHHHHHCEGHHCHFGHCAFEFHHHGGAEGGHHDHHHBGHBGHHH?5BGE1AEEFHHHHHGHFHBGHGEGGGGHBFHHFGHBGCFFG4EGHGG?E/FG3F3F/FGCDECEA/FGG/CEDBB//?BHEHHF0ADC@D>D9@DDCFFFFFFCFDFE.AA;/BB/.;-.;BFBBBFFBFFFFFFF/9FF?/;/BBFFFFF/;FFB
-@M01368:8:000000000-A3GHV:1:1106:17389:18671/2
-GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTCTTTAGGGTTTATGACC
-+
-BBCCCFFFCBCCGGGGGGGGGGHGHGHHGFGFGFHGGHHGHHHHHHHHFHHGGGGAGFBGHHHFBBGH5GDHH5EEEGEHEGFBF1DGFDGFGGGBBCEEGEHFEFDDHFBFHFD/@BGDGDHHGDCCFCD/C<->.ADDGE1GA;9.FFFFFFD-99ADFFD-.9FADF--9..;AAFEFFEAA-;.///BB:BBF/BBB//B//99/;BBB/9////F
-@M01368:8:000000000-A3GHV:1:1107:18714:24788/2
-GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACC
-+
-BCCCCCFFFFFFGGGGGGGEGGHHHHGGFFHHHHHHHHHHGGGGGHHHHGHHHHHHHHHHHHHFHHHHHHHHGHHHHHHGHHHHGGCGHHHHHHHHHHHHHHHGHHHHHGDGHGGHHHHHHHHGHHHHHHHHHHHGHHHHHHEHHHGHFGGGGHHHHEHFHHGHFHHF.>GGHHHGHGHGGHCFFCDFDFFFFFFAAF.FFFFFEFAFCFFA..@.AFFFC9--9;.
-@M01368:8:000000000-A3GHV:1:1107:21355:8333/2
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCGGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTA
-+
-AABCCFCCCCCFGGGGGGGGGGHHHGHHHFHHHHHHGHGHHHHHHGHHHHGGGGHHHHHHHHHFFHHFHGHHHHHHGGHGGHHHGHHHHGGHFHHHHHHHFHHGHHGEFFHFHHGGGGGGHHHFHHHHGHHHHE?FGHHHHGFGHGHHGGGDGGGGGFFFCFFFFFFFFFFFFA;DBBDFFFAFFFF;DBAFEDFFFFFEA?-9...9;9;BFFFF//BFBFFFFBFBFFFFFB/BFFF9B/EDEF-BF
-@M01368:8:000000000-A3GHV:1:1107:22864:15712/2
-GTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATT
-+
-AABCCFFFFFFFGGGGGGGGGGGGGHHHHHHHHHHGHHHGGGGFHGHHHHGHFGFFFFGGHGGGGGCHFHFAFEDHHGGFGGGHHEGEGGHHEGGCHGHHHHGGGGGGHGF0EECGGGGFECCFFGFBGHHHHHHGGHHGHFCFEHFBEFGGADDDC=CC0CCFHG:CEHHHGHBGH:GFFGFF0CAAA/BF09BBBBB
-@M01368:8:000000000-A3GHV:1:1107:24817:14233/2
-CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAAAGTGTGGGCTATTTAGGTTT
-+
-CCCCBFFFFFFFGGGGGGGGGGFHHHHHHHHHHHHHHGHHHGEHGGHHGHHHHHGHHEHHGGGGEHGHHHGEHHGHHDGHHHHGGGGGFGEFGGEGHHGCEEEGHHEFGGDHGGGGAGFHHHHGGF/?//GHFFDGGGGGHAD??DC1DHHBGHHHHGHF</ACFHHCDCDE?CGEBEGFFFGF?.?DGGGGEFEFFB00CFFFB.:AFFB;/BB9BBFFBBFFFAD.;///9.A./A:FB;BF//B
-@M01368:8:000000000-A3GHV:1:1107:24830:14440/2
-CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTAGTTAGGTTTT
-+
-CBCCCFFFFFFFGDGGGGGGGGHHHHHHHFGHHHHHHGHHHGGHGGHHHHHHGHHHFHHHGGGGGHHHHHGHHHHHHHHHHHHGGGGGHGHHFGGHHHGGGCGGGHHGGG?GGFGGGHHFHGH?FG/CDHHFAAEGGG/DHFC?-.AFGFH0CHHFH0DHHG/CDEBFHG.CA.:0C:;.FGFEFFG:E.B909BFF/;CFGBB9BBA.:CFGFFFFFFFF/;//AF?.ADEAB.9.A/;.:BFFF/9FF
-@M01368:8:000000000-A3GHV:1:1107:26478:23602/2
-ATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGCGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTTAGC
-+
-AAA?AFFFFFF>FGGGGGGGGGGGFHHEFGHHHGGGGGGHHHCGGEFHFGGGFHHHHHGGGEGGHHFHEEGEG/EEEE/?/EHFHH3GGH4EGHHCB01CGHGHGD//<@CGGBFFDGHHHH0FEHHGEGFHCD11>0=GGHGCCFGHGBGGD0GGHC0C.@?ACEEC/.EDEB?BFFCFB9CFF090BBCBFBF0CCB0B00A.9E.B/9BA=-..B9FBF//;BBBBFF
-@M01368:8:000000000-A3GHV:1:1108:11059:23181/2
-AACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCAC
-+
-CCCCCFFFFFCCGGGGGGGGGGHHHHHHHHHHHGHHHHHHHHGHHHHHHHHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHHHHHHHFHHHHHHHHHHHHHHHHGHGHEHHGFCGGGHHHHHHHHHHHHHHHFGHHHHHGHGHGHHGGGBFGFFFFEAFFFA.BFFFEFDDFFFFFFFFFFEEFF;D;EFFFFFF0BFFFADDFFFFFBFBFFFFFBB0FFFF0::0BF000BFFFBBF0EDFF
-@M01368:8:000000000-A3GHV:1:1108:12233:13498/2
-GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTA
-+
-ABBBCFFFFFFFGGGGGGGGGGHHHGHHHHHHBGHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHGHHHHHGGHHGHHHHHHGHHHHHHHGGHHHHGGGGGHHHHHHHHHHHHHGHGHHHHHHGHGHGGHGGGGAGGFGGGGGGGGFFFFFFFFFFDFFFFDFFFFFFFFF>.AEDEFFFFF?E-DAB9FFFFFFFEF:FBFB009:0FFFF9BFFBFFFFBB0.9A=-/0
-@M01368:8:000000000-A3GHV:1:1108:14328:14751/2
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTAT
-+
-ABBBBFBBBBBFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHDHHHHGGGGHHHHHHHHHHHHGHHHHHHHHHGGHGGHGGHHHHHGHHHHHHHHHHHHHHHHFHGHHHHHGGGGGHHHHHGHGHHHHHHG?FHHHFHGHGHGHHGGGGGGGGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFAFFE.ADEFFFFFFFADFFFFF///BBFF/FFFFFBFAFFFFBB///;9:
-@M01368:8:000000000-A3GHV:1:1108:16350:22621/2
-CATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATAT
-+
-CCCCCFFFFFFFGGGGGCGGGGHHHHHHHHGHHHHGHGHHHHGGGGHHHHHHHHHHHIHHHGHHHHHHGGHGGHHHHGHGHGHHHHHHHHHHHGHHHHHHGGHHHHGGGGGHHHHHHHHHHHHHGHGHHHGHHGGGGGHHGGGDADDGFFGGGGFFFFFFFFFDFFFDFFFFFFFFFFFAFAEFEDFFFFFFEE-AAFFFFFBFFFFF0FFFBFF;AFFFFFFFFFFFFFFFFFFFFFAB9BFFFFFBFFF
-@M01368:8:000000000-A3GHV:1:1108:21791:23272/2
-CATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTAT
-+
-BBBBBFFFFFFFGGGGGGGGGGHHHHGGHHHHHHHGGGEGGHHFGDHGHHHHHHGGHHH2GGHHHGHHGGGGFGFBFHHHGGGGHGHGHHHHHHHHHHGHGHHGHHGGHGGHHHHGGFHGGGHFHGHHHHHGGHHHGHGFCGFFDGGG/FCFHBGHHHHHHHGHCHHHHGGGHGHGHGCFF;>@DFFBFFFFFFDFFFFFFFF;;.DDAA;FFF;BB;C@..9::BBFBBA;-:.::
-@M01368:8:000000000-A3GHV:1:1108:23314:26089/2
-CATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATT
-+
-BBAAAFFFFFFFGGGGGGGGGGHHGDHHHHHFHHHHGGGGGFHFFGFFHEGHHHHHHHHFGHFHHHHDEHHHHHHGHFHHGGGGHHGFHHHHHHHHHHHHHHHHHHF?EEFCHFGFHHEFFFHGFHHGHFHFHHHFFHAG??GHACGGDGHGGBFHHHHHHHGHFGHGHGEGHGCFFF@CD-BDDFFFFFFFFDDFFBBFFD-B-A.DFFA.AFEFF-;-..:..BFFFFFA-../9FFF//B/BBBBF
-@M01368:8:000000000-A3GHV:1:1108:25084:18127/2
-CCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAG
-+
-CCCCCFFFFFFFGGGGGGGGGGGGHHHHHHHHFHHHHHHHHHHHHHHGGHHGHHHHHGGGGFHHHHHFHHHHHHHHHGGHHHHHCGGHGHHGGGGGGGHHGGGGGGGGHHHGHHGGGGGGGGGGGGGGGG@CDE?EFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFFFE;DFBFFFFFFFFFFFFFFFFFFFAFFFFFFFBFFE0BBBFFFFFFFFFFBFFFF9BB0:0:B;
-@M01368:8:000000000-A3GHV:1:1108:7626:10048/2
-AACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGAT
-+
-BCCDDFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHHHHHHHGGGGGHHHHHHHHFGHHHHHHHHHHHHGHHHHGGFHHHFGGGGHHHGHHHHHHHHHHHFGHHHHHGHGHGHHGCGCGGFHHHGGGGGGGGGGGGGGGGGGGFFFAFFFFFFAA-AFEFFFFFFFFFFFFFFFFFFFFFFFFF
-@M01368:8:000000000-A3GHV:1:1109:11270:12255/2
-CTACCTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATT
-+
-BCCCCFFFFFFFGGGGGGGGGGHHHHHFHHHHGGGGHHHHHHHHHHHHHHHHHHHHGHGGHGGHHGHHHHHGHHHHHHHHHFHHHHHGHHGHHHHHGGGGGHHHHHHHHGHHHHHH?FHHHHHGHGHGHHGGGGGGGGGGGGGGGGGGGGFFFFFFFFFFFFFFFFECADFFFF.DEBFFFFFF;D.B;.ED.FFFFFFF-.:00
-@M01368:8:000000000-A3GHV:1:1109:24217:19077/2
-TAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGAACCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTT
-+
-BBBBBFFFFFFFGFEGGCFGFGHHHHFHHFHHHHHHGGEEEFGHGFFAGFFEEAEGGHHBEEFAFGAEF1G3GFGGEEF@EAGGHGFF?ECGGHEEEFGGHHHHFHHFHHGFHHHC2DAGHGG00-BFFA9FFFFFFFFFFFBF
-@M01368:8:000000000-A3GHV:1:1109:27103:7153/2
-NTACTTCAGGGTCNTAAAACCTNAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCANGGNAGCTCTCCNNGNNNNTGGTATTNTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTAC
-+
-#>>AAAFFFFFFG#AAFFGGGG#BAFFAGHHHGGGGHGHHHHFHHHHHHHGHHHHHHHEGFGGHHBGHGHHBGFFEG2GHHFHFHHHGHHGHFH#/B#??FFGHHH##?####????FHG#??CFGGGEF>CGAGAGGGFGAA@@FFFFFFFFB=@@FF?FF;FFFF@@-@-AFEF?BB/BF?E-BBFFF;:BB/BFFFB/BFFFBFBFBBFFFFFB/FFFBB/;9A@@BFFBFFFF/BBFFFF
-@M01368:8:000000000-A3GHV:1:1109:8763:7859/2
-GGTTCCTACTTCAGGGTCATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAG
-+
->AAABFFFFFFFGGGGGGGGGGHHHHHHHCHFAFHHDGEGFHHHHHHHHFHHHHFHHHHHHGHGGHGGHHHFHGHHHHHFFHHHHHHBGHFFHGHGHHHHHGGEGGHHHHHGHHHHHHHHHGFGHHHHGHGHGHHCCGGGGGGGGGGAAGF?FFFFBFA@=BDDFF?-;FFF;@CFFEFEFFFFFFFFFBFDFFFFFFFFFFFFFFFFFFFFFF:FFFF/BF/FFFEFF?DECAFEFFFFFE:FFFFFFFB
-@M01368:8:000000000-A3GHV:1:1110:12808:18367/2
-GCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACAGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATT
-+
-ABCCCCDDDFFFGGGGGGGGGGHHHHHHHHHGGGGGGHHHHFHHGHHHHHHHHHHHHHGHHHGGHHHH3GFHEFHHHHHGHHGFHHHHHFGHHHHHGHGHGGHGGGGGGGHHHGGCGGGGHHGGGGGGGGGGGGGGGGGGGF-BAEFEBEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFFAFEFDFFFFFFFFFF0BFBED=@DFFFFFFFFFEFFFFFBFAFB;0:
-@M01368:8:000000000-A3GHV:1:1110:14751:26540/2
-ATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGGTTGGGCTATTTGGGTTTTTTGACC
-+
-BBBBBFBBBBBFGGGGGGGGGGHHHHGGFHGGHGGCHHHHHHGHHHGHHGEGGGHHHHHHHHHHEFHHHHHHGEE?FGHGEHGGHHHGGGGGGEHHGGGGHHGGGEGHHHGHGGGDFGHHGHGGGGGCGAFFCCDFHHHH1CGGHBHFHFCGFFCDFHGCCE.CC:EGFHHCGHHGCF?FGGGFGGG;B9;FFFFFFEGEBF0;;;0;CFBFFF?D.DE.:..99-...;;//9A.;DE.9-./:
-@M01368:8:000000000-A3GHV:1:1110:15519:10008/2
-GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACA
-+
-BBCBCFFFFFFFGGGGGGGGGGHHHGHHHFHHBGHHHGGGGHHHHHHGHHHHHHHHHHHHHHHGGHGGHHGHHHGGBGHHHHHHHHHHHHHHHHHGGFHHHGGGEGHHHHHHHHHHHHHHHGHHHHHHGHGHGHHGGGGGGDGGGGGGGGGBFFFFFFFFFFFFFFFFFFFECB-=BFFFFFFFFBB?E-A::FFBFFFFFBFFFFFFFFFFFFBFFEFFF;0BFF0:FE?FFFFBFFFFFFFFFFFFFF
-@M01368:8:000000000-A3GHV:1:1110:16559:7507/2
-GCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAAT
-+
->AABBCCCCFFFGGGGGGGGGGHHHHHHHHHGGHGGHGHFHHHHFHHHHHHHHHHFHHGHHHHGHHHHHGGEEGHHHHHHHHHHHHHHHHHHHHHHGHGHGHHGGGGGGGHHGGGFGAGFGGGGGGGGGGGDDDFAGGFFFFCFAFEEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFFFFFFFFFFFFFFE..@BFFBD./;:0FFFFF09B/AFCA=-99BFFBFFFFFFFB:BFBFFBFB9:
-@M01368:8:000000000-A3GHV:1:1110:16700:6843/2
-GTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTT
-+
-CBCCDFFFFFFFGGGGGGGGGGHHGHHHGGHFHHHGGGGGHHHHHHHHHHGHHHHHHHHHHHGGHGGHHHHHHHGBGFFHHHHHHGHHHHHHHHGHHHHHGGGGGHHHHHHHHHHHHHHHFHHHHGHCG/GGHHGGGGGGAFGEEADAGGDFFFF00D>CFDDFFFB?AF?B;-@>.FEDFFFFF0BF-BDFFFFE00B0BF0FFFF:BFA0B0:BBFBBFFFFF
-@M01368:8:000000000-A3GHV:1:1110:18014:7856/2
-CATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTAT
-+
-CDDDBFCDDDDFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHGGGGGHHHHHHHHHGHHHHHEGHHHHHEHGHGHHGGGCCGGHGHGCGGGGDHHHHFHGGGGGGGGGGGFGGFGAC-EFEGGGFFFGFF;DFFFBFFFFFFFFFFFFFFFFFFFEFFFBFFFFFFFFF
-@M01368:8:000000000-A3GHV:1:1110:23303:11846/2
-GTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTAAGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTA
-+
-BBCBBFFFFFCFGGGGGGGGGGHHHHHHGGHHHHHHHHGH5GHHHGGGGHHHHHFHHHHHHHEHHGFBGEEFE1FG5GFHHHHHFHHHGGGGGHHHHHHHHHHHGGHHHHHGGGGFHHFHH/EHHHGG?@EFHHHGGGGHFGGGGHHFHGGDGGGGFFFHGFDGGGCGACC;DFFB0CH0CCCFGBFBFB/:A?FFFE?AA.CFBECE0BBBF0F/A./9FFFFF./;/FFFFFFA.:////9BB/B/BB
-@M01368:8:000000000-A3GHV:1:1111:13195:14562/2
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGAT
-+
-ABBBBFBBBBBFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHDGHHHGGGGHHHHHHHHHHHHGHHHHHHHHHGGGGGHHGHHHHGFGFHHHHHHHHGHHHHHHHGHHHHHGGGGGHHHHGHHHHHHHHHHGFHHHHHGFGHGHHGGGGFCGFGFFFFAFFFFFFFFFFA;BADFFFAFFFFFFFFFEFFFEFFFFFFF;FDBFFFFFFFFFFFF
-@M01368:8:000000000-A3GHV:1:1111:14287:20817/2
-ATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATGAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGTTAGACCTGTGATC
-+
-CCCCBFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHHHHHHHHHGHHHGHHHHGHHHGHGHFHHHGGGGHHHHHHHHBGGGFHHFGGHHGGHGHHHHHHHHEGFFHHFGGGGGHHEHHHBHHHHHFFHHGHGGGGGHFGF3FDGHHGCGGFGHHGGGG/2GG@E?GHHHGFEDGGCFDFFFFFFFFFFFBFFFFFFFFFFF/FFF/BFFFFFFFBA.?@;FFBDFFFF
-@M01368:8:000000000-A3GHV:1:1111:7437:24508/2
-ACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGT
-+
-BBBBBFFBABBBGGGGGGGGFGHHHHHHHHHHHHHGHHHGFHHHHGGGGDHHHHHHHHGFHHHHHGHHHHHHGHFHGHHGGGDFGDFFGGGFFGFFGHGHHHGGC?AD-:A-AFFGGG-?9EFEFGGFFBFEFCFFFFFFFBBBFFFFFFFFFFFFFFFBBFFFFFFFFFFFFFFFFFFEFFFFFFBFFFFEFEFFFF;CF-FFFFFFFF/BFFFFFFF
-@M01368:8:000000000-A3GHV:1:1111:8665:25542/2
-CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGACAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTT
-+
-CCCCCFFFFFFFGGGGGGGGGGHHHHHHHFHHHHCHHGDGGGGHGGHHHHHHHHGHGHHHGGGGGHFHHHHFHHHFFHHHHHFGCGGEHFHHFGGHFHGGGGGGHHFEEEAFEFFDFHGHHHHGGGGGGGHH0DGGGGCGHCC-FFFFFFFGGGGGGGGGGHHHHHGHHHHGHHGGHHGGHGGHHHGHHHHFHHGHHAFGGGHHHHHGHHHHFHFHHHHHECGGGHGHHHEFHFHGGGGGGGGFGFFGFFEFGEGHHHGHHGGDGGGHGFGGGGGGGGE@GCGGHHHGHHHHHGHHHHFHHGEGFFEGCE.ECCECEHCGGFFE/AABFFG0CFE0BFFF9CFGG.F/BBBFFGFGGGFFFFFFBFEDAEFFFFFBFFFFFFF0;FEBF:
-@M01368:8:000000000-A3GHV:1:1112:10272:7828/2
-GTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATAC
-+
-BBCCBFFCCCCCGGGGGGGGGGGGGGHHHHHHGGGGGGHHFHGGGGGFGHGGGGGGHHHHHHHHHHFHHHHFHGFFHHGF?ECEFFFFFFHHHHHH?FHHHHHHHHHGFHHHHHHGGGFGHHHHFHHHHHHHHHHGGGFGFGCGGGGHHHHHHHHHHHGGFFHGHHHHHFFHFCGFHCCGHGHCGGHG?DGG
-@M01368:8:000000000-A3GHV:1:1112:9832:16531/2
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTA
-+
-BBBBBFBBBBBFGGGGGGGGGGHFHHHHHHHHHHHHHHGHHHHGHFHHHGEEGGHHHGHHHHHHHHHHHHEHGHHGGGHGGHGHHHHHGHHHHGHHHHHHHHHHHHHHGHHHHHGEGCGHHHHHGHHHHHHHHHHHHHHGHFFGHGHHGGGDACDFFFFABFFFFFFFFFFD-BC?DA9.DFFBEA==B=?F.;ABBFBFEF-DAF/BFF9/FFFBB/9BFFFFFFF/BFBBFFFFFFF/9FEA?--B/
-@M01368:8:000000000-A3GHV:1:1112:9832:6701/2
-GCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATT
-+
-BBBBAFFFFFFFGGGGGGGGGGFGHHHHHGHHHHHHHHHHHHCGHHHHHHHGHHHHHHDGHHGGAECHHGHHHHHHHGHHHGHGFHHHHGGHGGHHFHHHHHHFHHHHHHHHH2GHHHHGHGHHFHHGGFGGGHHGHHHHHFHHHHEFHHHHEFGFFHGEHCFGDFDFFFFFCFFFFFFFFFFFFFAFBFFDFFFFFFFFADCF?9?EFBFB9EF-A.;BFFFFFFFBFFFFFFFFFFFFBFFFFFFFFFF
-@M01368:8:000000000-A3GHV:1:1113:13532:8618/2
-GCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTGGAT
-+
-CDDDBFFFFFFFGGGGGGGGGGHHHHHGGHHHGHHHGHHGGGGEHHGGHHHHHHHHHHHHHHGHHGHHHHHGGHHHHHFHHHHGCDCGHHHHHHHHEHHHGHGHHHGFHGGHGGHHHHHHHHFHHHFHHHGHHHHHHHHHFGGHHHHGGGEGAFHHGHHGGHHHHGHFBGFHGHGHGHGGHGGF@DAFEFBECAB=A.0BFFFEDF=A9.@D.-AAFFFA-:-..:.9/0:;0..-..0000::BFB09::
-@M01368:8:000000000-A3GHV:1:1113:21679:18011/2
-TTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATC
-+
-BCCCBFFFFFFFGGGGGGGGGGHHHHHFHHHGHHHGFGHGGHGGHHHHHHGHHHHHHHGGGGFHHHHHHEGHHHHHHHHFGGFCGEHFHFGEGHHHFGGGGGHHHGGGEHGGGGGHHHHHHGDCCCGHHHHDGGGGCD/FCC->DFF
-@M01368:8:000000000-A3GHV:1:1113:5741:16959/2
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTA
-+
-BBBBCFCCCCCFGGGGGGGGGGHHHHHHHGHHHHHHHHGHHHHGHDGHHHGGGGHHHHHHHHHHHHHHHGHHHHHGGGEGGHHHHFHHHHHHHHHHHHHHHHHHHHHHGHHGHHGGGGGHHHHHHHHHHHGHHHFHHHHHHGHGHGHGGGGCGGFGGFFFFFFFFFFFFFBFFFFFFFFFFFFFFAFFFFEAEFFFFFFFFFFF9BFFBFFFFFFFFFFFFFFBFFFFFFFFFFFFFFFFFFADAB-/B
-@M01368:8:000000000-A3GHV:1:1114:10130:11959/2
-ATCAGAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACG
-+
-BCCDDFFFFFFFGGGGGGGGGGGHHHHHGGHHHGGGCGGHGHGGGGHHGGGGHHHHHHGGGEGGHHHFGGGGG?E1FE?/EEHHHHHGHHGHHHHGHFHGHGHHGDGGFG2FF2?GHHHHHGCCCFHGHGHHHHGHHFEHHFGHHGHH<1=DGHHHGHHGHGAGAE
-@M01368:8:000000000-A3GHV:1:1114:14540:5315/2
-CACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGC
-+
-CCCCCFFCCDCCGGGGGGGGGGHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHHGHHHHHHHGHHHHFHFHHHGGGGGHHHHHHHHHHHHHGHHHHHHHHGGGGGGHHHFHHHGHHHHHHHHHHHHHHHHHHGHGHHHGGGFGHHHHHHHHFHHHHHF?1FHHGHGHGHGHHGGFFFFDBFBE;BCC.:BFFFFFFFFFFFFFF;AFFFFF-=-.AEDEFFFFF..9A;9FFFF0FFFFE00FFF0:B
-@M01368:8:000000000-A3GHV:1:1114:15066:16302/2
-TTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAG
-+
-CCCCCFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHHHDGHHHHFHHGHHGHHHHHGGGGEHHHHFHHFF5FHHFEGHHHGDHGGHGHGFGGGEHFGHHGGGGGHGHHHGHHFHHB3FGHHFGGGG?GFFHCCEBGFFECCDFEGFCFGCHHGFDDHHHGHHCFGGGGGFBFDGFG?-:..AFG.-C0C009;:00;00:9/:CEFFF?AE::9;9?0:FEF0;
-@M01368:8:000000000-A3GHV:1:1114:16639:15258/2
-TTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCGCACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGAT
-+
-CDCCCFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHGHHHHHHGGGGGHGIHHHIH5DEGHHHF?FGHGGHGGHEGGHFHHGHGEHHGGGGGFFFGHFBG2GHEBGHHGHGGEG/GFGABEDFGHEED?GGHHFFGGGCFEGD/GFHFFGEFGCGG?CC??D-EF@EEEFGCDDBBFGGGEBBFFF09090A.BFGA.9CCA0;EBAB00BBFF.@-./;BB;BFFF0:00099AAFFF
-@M01368:8:000000000-A3GHV:1:1114:2404:13066/2
-ATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGACCATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGA
-+
-CCCCCFFFFFFCGGGGGGGGGGHHHHHHHHHFFHHHHGGGGGHFFFHHFHHHHHHHHHHHHHHHGFEGGGHGEDFCDFHGHFG@@DGGHHHHHHGGGGCGGGGGEHGGCGBB?CF99EGFGGFGG?D9CFFFF/BBFFFFFEF9BFFAFFFFEFFFFFFFFFFFFFFFFFFFFF.FFBBFFFFFFFFFFFF-9;;;BFFFFFB9BFBFBFABFFEFFFFFFFFFF::BFFBFF
diff --git a/tools/trim_galore/test-data/paired_collection_example_results3.txt b/tools/trim_galore/test-data/paired_collection_example_results3.txt
index c910fdb35a..b30f76de20 100644
--- a/tools/trim_galore/test-data/paired_collection_example_results3.txt
+++ b/tools/trim_galore/test-data/paired_collection_example_results3.txt
@@ -1,52 +1,38 @@
-
SUMMARISING RUN PARAMETERS
-==========================
+=========================
Input filename: input_1.fastq
Trimming mode: paired-end
-Trim Galore version: 0.6.10
-Cutadapt version: 5.0
-Python version: 3.12.10
-Number of cores used for trimming: 4
+Trim Galore version: 2.2.0 (Oxidized Edition)
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
-Using Nextera adapter for trimming (count: 29). Second best hit was Illumina (count: 0)
-Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected)
-Maximum trimming error rate: 0.1 (default)
+Adapter sequence: 'CTGTCTCTTATA' (user-specified or auto-detected)
+Maximum trimming error rate: 0.1
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp
-Length cut-off for read 1: 35 bp (default)
-Length cut-off for read 2: 35 bp (default)
-This is cutadapt 5.0 with Python 3.12.10
-Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq
-Processing single-end reads on 4 cores ...
+Trim Galore 2.2.0 (Oxidized Edition) — adapter trimming built in
+This is cutadapt 4.0 (compatible; for MultiQC backwards compatibility)
+Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq
+Processing reads on 1 core in single-end mode ...
=== Summary ===
-Total reads processed: 99
-Reads with adapters: 52 (52.5%)
-Reads written (passing filters): 99 (100.0%)
+Total reads processed: 99
+Reads with adapters: 52 (52.5%)
+Reads written (passing filters): 99 (100.0%)
-Total basepairs processed: 24,849 bp
-Quality-trimmed: 205 bp (0.8%)
-Total written (filtered): 23,339 bp (93.9%)
+Total basepairs processed: 24,849 bp
+Quality-trimmed: 205 bp (0.8%)
+Total written (filtered): 23,339 bp (93.9%)
=== Adapter 1 ===
-Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times
+Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times.
-Minimum overlap: 1
No. of allowed errors:
1-9 bp: 0; 10-12 bp: 1
-Bases preceding removed adapters:
- A: 9.6%
- C: 38.5%
- G: 23.1%
- T: 28.8%
- none/other: 0.0%
-
Overview of removed sequences
length count expect max.err error counts
1 11 24.8 0 11
@@ -57,80 +43,70 @@ length count expect max.err error counts
13 2 0.0 1 2
14 1 0.0 1 1
16 1 0.0 1 1
-17 1 0.0 1 0 1
-20 2 0.0 1 2
-21 1 0.0 1 1
-24 1 0.0 1 1
-26 2 0.0 1 2
-31 1 0.0 1 1
-33 1 0.0 1 1
-41 2 0.0 1 2
-49 1 0.0 1 1
-50 1 0.0 1 1
-54 1 0.0 1 1
-56 1 0.0 1 1
-58 2 0.0 1 2
-60 1 0.0 1 1
-67 2 0.0 1 2
-68 1 0.0 1 1
-69 1 0.0 1 1
-73 1 0.0 1 1
-80 1 0.0 1 1
-86 1 0.0 1 1
+17 1 0.0 1 1
+20 2 0.0 2 2
+21 1 0.0 2 1
+24 1 0.0 2 1
+26 2 0.0 2 2
+31 1 0.0 3 1
+33 1 0.0 3 1
+41 2 0.0 4 2
+49 1 0.0 4 1
+50 1 0.0 5 1
+54 1 0.0 5 1
+56 1 0.0 5 1
+58 2 0.0 5 2
+60 1 0.0 6 1
+67 2 0.0 6 2
+68 1 0.0 6 1
+69 1 0.0 6 1
+73 1 0.0 7 1
+80 1 0.0 8 1
+86 1 0.0 8 1
+
RUN STATISTICS FOR INPUT FILE: input_1.fastq
=============================================
99 sequences processed in total
-
+Sequences removed because they became shorter than the length cutoff of 20 bp: 1 (1.0%)
+Sequences removed because they were longer than the upper length cutoff: 0 (0.0%)
+Sequences removed because of too many N bases: 0 (0.0%)
SUMMARISING RUN PARAMETERS
-==========================
+=========================
Input filename: input_2.fastq
Trimming mode: paired-end
-Trim Galore version: 0.6.10
-Cutadapt version: 5.0
-Python version: 3.12.10
-Number of cores used for trimming: 4
+Trim Galore version: 2.2.0 (Oxidized Edition)
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
-Using Nextera adapter for trimming (count: 29). Second best hit was Illumina (count: 0)
-Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected)
-Maximum trimming error rate: 0.1 (default)
+Adapter sequence: 'CTGTCTCTTATA' (user-specified or auto-detected)
+Maximum trimming error rate: 0.1
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp
-Length cut-off for read 1: 35 bp (default)
-Length cut-off for read 2: 35 bp (default)
-This is cutadapt 5.0 with Python 3.12.10
-Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq
-Processing single-end reads on 4 cores ...
+Trim Galore 2.2.0 (Oxidized Edition) — adapter trimming built in
+This is cutadapt 4.0 (compatible; for MultiQC backwards compatibility)
+Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq
+Processing reads on 1 core in single-end mode ...
=== Summary ===
-Total reads processed: 99
-Reads with adapters: 58 (58.6%)
-Reads written (passing filters): 99 (100.0%)
+Total reads processed: 99
+Reads with adapters: 58 (58.6%)
+Reads written (passing filters): 99 (100.0%)
-Total basepairs processed: 24,849 bp
-Quality-trimmed: 745 bp (3.0%)
-Total written (filtered): 23,035 bp (92.7%)
+Total basepairs processed: 24,849 bp
+Quality-trimmed: 745 bp (3.0%)
+Total written (filtered): 23,035 bp (92.7%)
=== Adapter 1 ===
-Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times
+Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times.
-Minimum overlap: 1
No. of allowed errors:
1-9 bp: 0; 10-12 bp: 1
-Bases preceding removed adapters:
- A: 12.1%
- C: 37.9%
- G: 8.6%
- T: 41.4%
- none/other: 0.0%
-
Overview of removed sequences
length count expect max.err error counts
1 16 24.8 0 16
@@ -146,29 +122,42 @@ length count expect max.err error counts
16 1 0.0 1 1
17 1 0.0 1 1
19 2 0.0 1 2
-21 1 0.0 1 1
-25 1 0.0 1 1
-30 1 0.0 1 1
-32 2 0.0 1 2
-34 1 0.0 1 1
-36 2 0.0 1 2
-38 1 0.0 1 1
-40 1 0.0 1 1
-41 1 0.0 1 1
-42 1 0.0 1 1
-43 1 0.0 1 1
-49 1 0.0 1 1
-51 1 0.0 1 1
-56 1 0.0 1 1
-57 1 0.0 1 1
-60 1 0.0 1 1
-67 1 0.0 1 1
-80 1 0.0 1 1
+21 1 0.0 2 1
+25 1 0.0 2 1
+30 1 0.0 3 1
+32 2 0.0 3 2
+34 1 0.0 3 1
+36 2 0.0 3 2
+38 1 0.0 3 1
+40 1 0.0 4 1
+41 1 0.0 4 1
+42 1 0.0 4 1
+43 1 0.0 4 1
+49 1 0.0 4 1
+51 1 0.0 5 1
+56 1 0.0 5 1
+57 1 0.0 5 1
+60 1 0.0 6 1
+67 1 0.0 6 1
+80 1 0.0 8 1
+
RUN STATISTICS FOR INPUT FILE: input_2.fastq
=============================================
99 sequences processed in total
+Sequences removed because they became shorter than the length cutoff of 20 bp: 1 (1.0%)
+Sequences removed because they were longer than the upper length cutoff: 0 (0.0%)
+Sequences removed because of too many N bases: 0 (0.0%)
+
+=== Paired-end validation ===
+
+Number of sequence pairs analysed: 99
+
+Number of sequence pairs removed because at least one read was shorter than the length cutoff (1.01%):
+ 1
+Number of pairs removed for N content (0.00%):
+ 0
-Total number of sequences analysed for the sequence pair length validation: 99
+Unpaired read 1 kept: 1
+Unpaired read 2 kept: 0
-Number of sequence pairs removed because at least one read was shorter than the length cutoff (20 bp): 1 (1.01%)
diff --git a/tools/trim_galore/test-data/paired_collection_example_results3gz.txt b/tools/trim_galore/test-data/paired_collection_example_results3gz.txt
index 3cd6bee72d..78e51ce226 100644
--- a/tools/trim_galore/test-data/paired_collection_example_results3gz.txt
+++ b/tools/trim_galore/test-data/paired_collection_example_results3gz.txt
@@ -1,53 +1,39 @@
-
SUMMARISING RUN PARAMETERS
-==========================
+=========================
Input filename: input_1.fastq.gz
Trimming mode: paired-end
-Trim Galore version: 0.6.10
-Cutadapt version: 5.0
-Python version: 3.12.10
-Number of cores used for trimming: 4
+Trim Galore version: 2.2.0 (Oxidized Edition)
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
-Using Nextera adapter for trimming (count: 29). Second best hit was smallRNA (count: 0)
-Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected)
-Maximum trimming error rate: 0.1 (default)
+Adapter sequence: 'CTGTCTCTTATA' (user-specified or auto-detected)
+Maximum trimming error rate: 0.1
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp
-Length cut-off for read 1: 35 bp (default)
-Length cut-off for read 2: 35 bp (default)
Output file will be GZIP compressed
-This is cutadapt 5.0 with Python 3.12.10
-Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq.gz
-Processing single-end reads on 4 cores ...
+Trim Galore 2.2.0 (Oxidized Edition) — adapter trimming built in
+This is cutadapt 4.0 (compatible; for MultiQC backwards compatibility)
+Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq.gz
+Processing reads on 1 core in single-end mode ...
=== Summary ===
-Total reads processed: 99
-Reads with adapters: 52 (52.5%)
-Reads written (passing filters): 99 (100.0%)
+Total reads processed: 99
+Reads with adapters: 52 (52.5%)
+Reads written (passing filters): 99 (100.0%)
-Total basepairs processed: 24,849 bp
-Quality-trimmed: 205 bp (0.8%)
-Total written (filtered): 23,339 bp (93.9%)
+Total basepairs processed: 24,849 bp
+Quality-trimmed: 205 bp (0.8%)
+Total written (filtered): 23,339 bp (93.9%)
=== Adapter 1 ===
-Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times
+Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times.
-Minimum overlap: 1
No. of allowed errors:
1-9 bp: 0; 10-12 bp: 1
-Bases preceding removed adapters:
- A: 9.6%
- C: 38.5%
- G: 23.1%
- T: 28.8%
- none/other: 0.0%
-
Overview of removed sequences
length count expect max.err error counts
1 11 24.8 0 11
@@ -58,81 +44,71 @@ length count expect max.err error counts
13 2 0.0 1 2
14 1 0.0 1 1
16 1 0.0 1 1
-17 1 0.0 1 0 1
-20 2 0.0 1 2
-21 1 0.0 1 1
-24 1 0.0 1 1
-26 2 0.0 1 2
-31 1 0.0 1 1
-33 1 0.0 1 1
-41 2 0.0 1 2
-49 1 0.0 1 1
-50 1 0.0 1 1
-54 1 0.0 1 1
-56 1 0.0 1 1
-58 2 0.0 1 2
-60 1 0.0 1 1
-67 2 0.0 1 2
-68 1 0.0 1 1
-69 1 0.0 1 1
-73 1 0.0 1 1
-80 1 0.0 1 1
-86 1 0.0 1 1
+17 1 0.0 1 1
+20 2 0.0 2 2
+21 1 0.0 2 1
+24 1 0.0 2 1
+26 2 0.0 2 2
+31 1 0.0 3 1
+33 1 0.0 3 1
+41 2 0.0 4 2
+49 1 0.0 4 1
+50 1 0.0 5 1
+54 1 0.0 5 1
+56 1 0.0 5 1
+58 2 0.0 5 2
+60 1 0.0 6 1
+67 2 0.0 6 2
+68 1 0.0 6 1
+69 1 0.0 6 1
+73 1 0.0 7 1
+80 1 0.0 8 1
+86 1 0.0 8 1
+
RUN STATISTICS FOR INPUT FILE: input_1.fastq.gz
=============================================
99 sequences processed in total
-
+Sequences removed because they became shorter than the length cutoff of 20 bp: 1 (1.0%)
+Sequences removed because they were longer than the upper length cutoff: 0 (0.0%)
+Sequences removed because of too many N bases: 0 (0.0%)
SUMMARISING RUN PARAMETERS
-==========================
+=========================
Input filename: input_2.fastq.gz
Trimming mode: paired-end
-Trim Galore version: 0.6.10
-Cutadapt version: 5.0
-Python version: 3.12.10
-Number of cores used for trimming: 4
+Trim Galore version: 2.2.0 (Oxidized Edition)
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
-Using Nextera adapter for trimming (count: 29). Second best hit was smallRNA (count: 0)
-Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected)
-Maximum trimming error rate: 0.1 (default)
+Adapter sequence: 'CTGTCTCTTATA' (user-specified or auto-detected)
+Maximum trimming error rate: 0.1
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp
-Length cut-off for read 1: 35 bp (default)
-Length cut-off for read 2: 35 bp (default)
Output file will be GZIP compressed
-This is cutadapt 5.0 with Python 3.12.10
-Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq.gz
-Processing single-end reads on 4 cores ...
+Trim Galore 2.2.0 (Oxidized Edition) — adapter trimming built in
+This is cutadapt 4.0 (compatible; for MultiQC backwards compatibility)
+Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq.gz
+Processing reads on 1 core in single-end mode ...
=== Summary ===
-Total reads processed: 99
-Reads with adapters: 58 (58.6%)
-Reads written (passing filters): 99 (100.0%)
+Total reads processed: 99
+Reads with adapters: 58 (58.6%)
+Reads written (passing filters): 99 (100.0%)
-Total basepairs processed: 24,849 bp
-Quality-trimmed: 745 bp (3.0%)
-Total written (filtered): 23,035 bp (92.7%)
+Total basepairs processed: 24,849 bp
+Quality-trimmed: 745 bp (3.0%)
+Total written (filtered): 23,035 bp (92.7%)
=== Adapter 1 ===
-Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times
+Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times.
-Minimum overlap: 1
No. of allowed errors:
1-9 bp: 0; 10-12 bp: 1
-Bases preceding removed adapters:
- A: 12.1%
- C: 37.9%
- G: 8.6%
- T: 41.4%
- none/other: 0.0%
-
Overview of removed sequences
length count expect max.err error counts
1 16 24.8 0 16
@@ -148,29 +124,42 @@ length count expect max.err error counts
16 1 0.0 1 1
17 1 0.0 1 1
19 2 0.0 1 2
-21 1 0.0 1 1
-25 1 0.0 1 1
-30 1 0.0 1 1
-32 2 0.0 1 2
-34 1 0.0 1 1
-36 2 0.0 1 2
-38 1 0.0 1 1
-40 1 0.0 1 1
-41 1 0.0 1 1
-42 1 0.0 1 1
-43 1 0.0 1 1
-49 1 0.0 1 1
-51 1 0.0 1 1
-56 1 0.0 1 1
-57 1 0.0 1 1
-60 1 0.0 1 1
-67 1 0.0 1 1
-80 1 0.0 1 1
+21 1 0.0 2 1
+25 1 0.0 2 1
+30 1 0.0 3 1
+32 2 0.0 3 2
+34 1 0.0 3 1
+36 2 0.0 3 2
+38 1 0.0 3 1
+40 1 0.0 4 1
+41 1 0.0 4 1
+42 1 0.0 4 1
+43 1 0.0 4 1
+49 1 0.0 4 1
+51 1 0.0 5 1
+56 1 0.0 5 1
+57 1 0.0 5 1
+60 1 0.0 6 1
+67 1 0.0 6 1
+80 1 0.0 8 1
+
RUN STATISTICS FOR INPUT FILE: input_2.fastq.gz
=============================================
99 sequences processed in total
+Sequences removed because they became shorter than the length cutoff of 20 bp: 1 (1.0%)
+Sequences removed because they were longer than the upper length cutoff: 0 (0.0%)
+Sequences removed because of too many N bases: 0 (0.0%)
+
+=== Paired-end validation ===
+
+Number of sequence pairs analysed: 99
+
+Number of sequence pairs removed because at least one read was shorter than the length cutoff (1.01%):
+ 1
+Number of pairs removed for N content (0.00%):
+ 0
-Total number of sequences analysed for the sequence pair length validation: 99
+Unpaired read 1 kept: 1
+Unpaired read 2 kept: 0
-Number of sequence pairs removed because at least one read was shorter than the length cutoff (20 bp): 1 (1.01%)
diff --git a/tools/trim_galore/test-data/paired_collection_example_unpair1_results3.fastq.gz b/tools/trim_galore/test-data/paired_collection_example_unpair1_results3.fastq.gz
index 34596d67c5..0a38706076 100644
Binary files a/tools/trim_galore/test-data/paired_collection_example_unpair1_results3.fastq.gz and b/tools/trim_galore/test-data/paired_collection_example_unpair1_results3.fastq.gz differ
diff --git a/tools/trim_galore/test-data/paired_collection_example_unpair1_results3.fastqsanger b/tools/trim_galore/test-data/paired_collection_example_unpair1_results3.fastqsanger
deleted file mode 100644
index a8fa963bf3..0000000000
--- a/tools/trim_galore/test-data/paired_collection_example_unpair1_results3.fastqsanger
+++ /dev/null
@@ -1,4 +0,0 @@
-@M01368:8:000000000-A3GHV:1:1101:6911:8255/1
-ATCTGGTTCCTACTTCAGGGCCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATT
-+
-BCCCCFFFFFFFGGGGGGGGGGGHHHHGHGHHHHHHHHHGGGGGGHHHHGHHHHHHHHHHGHHHHHHGGHGGHHHGHHHHFHHGHHHHHHHHHGHEHEFFGHHEGGCEFGGFHHHBGHHGHHHHGHFHHHGHGHGHGGCDFDDACGGGGGGGAAFFFFFFFFFBAFFFFFB;FFFFFFADDFFFFFFFFFFEFFFFFFFFFFBFFFFFFFFFFFFFFEFFFFFFFFBFEFFFFEFE;DFFFDFBFF/9BFB
diff --git a/tools/trim_galore/test-data/paired_collection_example_unpair2_results3.fastq.gz b/tools/trim_galore/test-data/paired_collection_example_unpair2_results3.fastq.gz
index 898d76873b..f9631332bb 100644
Binary files a/tools/trim_galore/test-data/paired_collection_example_unpair2_results3.fastq.gz and b/tools/trim_galore/test-data/paired_collection_example_unpair2_results3.fastq.gz differ
diff --git a/tools/trim_galore/test-data/paired_collection_example_unpair2_results3.fastqsanger b/tools/trim_galore/test-data/paired_collection_example_unpair2_results3.fastqsanger
deleted file mode 100644
index e69de29bb2..0000000000
diff --git a/tools/trim_galore/test-data/paired_example_pair1_results2.fastq.gz b/tools/trim_galore/test-data/paired_example_pair1_results2.fastq.gz
index 953dc1fce8..ff0dc41d36 100644
Binary files a/tools/trim_galore/test-data/paired_example_pair1_results2.fastq.gz and b/tools/trim_galore/test-data/paired_example_pair1_results2.fastq.gz differ
diff --git a/tools/trim_galore/test-data/paired_example_pair1_results2.fastqsanger b/tools/trim_galore/test-data/paired_example_pair1_results2.fastqsanger
deleted file mode 100644
index 0d55c14fd4..0000000000
--- a/tools/trim_galore/test-data/paired_example_pair1_results2.fastqsanger
+++ /dev/null
@@ -1,392 +0,0 @@
-@M01368:8:000000000-A3GHV:1:1101:14518:9998/1
-GTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTG
-+
-AAAAAFFFFFFFGGGGGGGGGGHGGHHHHGHHHHHHHGCGHHHHHHHHHHHHHHHGGGGGHHHHHHHHHGHHGFHFE5BGEEHFGGGHHHHHHHHFBHHGGGGFHGHHFGHHHHGHHHHHHGEGGGGFHFHGEGHHGGCDGDGHGGGDGGHGGCGGGHGHHH/ACDG?.1FGCDCCGCA.CC@CDCHFHGFFGGGEBFGAB//CEFBFGG.:;D;;A0AFFFFFB..:@ABFF//;BFFFFFBF/9D:A
-@M01368:8:000000000-A3GHV:1:1101:18422:19051/1
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTA
-+
-CCCCCFDDDDDFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHFHHHHGGGGHHHHHHHHHGHHHHHHHHHHHHGGHGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHGCGGGHHHHHHHHHHHHHHHHHHHHHHGFDHGFHCFGGGGFGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF;FFFFFFFFFFFFFFFFFFFFFFFFFFFFEFBFFFFFFFFFF:FF
-@M01368:8:000000000-A3GHV:1:1101:25545:21098/1
-ATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATAAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGGCTTATTTAAGGGGAACGGGTGG
-+
-33AA?DFD5BDFGGGFEBDGEGHEGHGEGHCEGGHHCHGHHFFHHGFGAGE53FF2FAFFGDE5FFFE5GFBFGAEE1GHHHGHHHEHE3FGHF@GEGEGGHHGG3FAGFFDE?EEE3GFEGFGFGGCG?GHHHFHGGGC@DHFFHD/AA@AAAAD2ADDFFGGGGGFGGHA?EAEFBEAGHFABFGG5FDF5DB1EEGAFDFB53FF5FH@G5FFEHGHEFHFFHBE333GF43GCGGGGE@0?BFGGB0B?FHGFDGGHHHBFFDEGGHGFFFDFE@<1>@FFFGHHHHFHEFGDABFFGG/@DCE;>=EF?AEFFFF;9;=DAFFFFBBFFFBFFF//;BFB.:FBFFBB/BFFFF/BFFE?EFAFEF9
-@M01368:8:000000000-A3GHV:1:1104:11928:24197/1
-ACGTAGGTGCGATAAATAATGGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATA
-+
-CDDCCFCFFFBCGGGGGGGGGGHHHGHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHGGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHGHHHHGHHGHHHHGGHGEEGGGGFHHFHHHGHGEHHHHHHHHHHHHHHHHHGGGGGGFGGGGGGGGFFGGGFGFGAEFEFGFEAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF@D9
-@M01368:8:000000000-A3GHV:1:1104:12288:20635/1
-CACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGCCGCAGCATCTGTCTTTGATTCCTGC
-+
-ABA?AFBAB2>AFGGGGFFGD5GGHGFFHGHEGGGGHHHHFFHH5FHGHHHFFHEFHHHHHHGGHHHHHGFHGGHGGGGFHGGGFBHHHFGEGHHHHGHGGGGECEHHFHHGHHFFHFGGGHHHEGHDGHHGHGHFHHHECGCBCHHGFHHFHD1GFHEFHEF?GDCFFEFE0A@;@>BD/9BFBBBBFDFFFF99D=?>DDBFF-AFFEBB9BBEFEFAFFF//-@---;;FBBFBFB///;99999B
-@M01368:8:000000000-A3GHV:1:1104:13832:4398/1
-CACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTC
-+
-AAABABCAFFFFGGGGGGGGGGHFHHHHHHHHHGFFHHGGHGGGGGHHHHHHHHHHFHHHHGHHHHHGGHGHGGHGGGGGGGHGFFGGGGDGGHHHHGGGGFGGGGGCFGGEGCDGGGGGGGGGGGGEGF?;BFFFFFF
-@M01368:8:000000000-A3GHV:1:1104:23670:4161/1
-ATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATC
-+
-AAAABFFFFFFFGGGGGGGCGBBGGHHHHHHHHHGHHHHHHHHHHFGGHHHHHHGGFHHHHHHHGGGGGFHHHGHHHHHGHHHHGHHGGGHGGGGHHHHHGHHHHHHEGGGFHHHGFFDGEGHGHFHGHHGEGGGHHBGHGGHGHGGGGG/@FCDFDGFHGGGDFFHFGFFGGGGGHHHHGGGGGGGEGCDG?GGBFFFEE00BFFGABBBAFGGGEGEA.9FFF/./;BFBF./AAF/BFFBBF/F/BF
-@M01368:8:000000000-A3GHV:1:1104:24927:22253/1
-AGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTAT
-+
-AAA?AFFF3DFFFGGBGFF3EAEEEHFHHFHHHGHHHHDGHHGAEFGHHHGFCHHGHHHBGHHHAEGGHGGFHFBFHHHHHHHHHHFHH1EEHEEHH1FFDEEFCGHBGHHFHEHHHHHH1FCGHHHHEEFGEEGFFCHHH11>GHHF/FG2DGGCGAGGHGGGG@@@BEFFF?@@@FFEFFFFFF?@@FB@BB@FFFE=;==---A9EFBBB/;E;A9B/B/FFFEF/B///;/;9/A/;/9BFFFBFBB
-@M01368:8:000000000-A3GHV:1:1104:4705:7727/1
-CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG
-+
-BBBBBFFBFFFFGGGGGGGGGGHHHGHHHGHHHHH6GHHHHFFGGGHHHHHHHHHHHHHHHGGFGGGEGGGHHHHHHHHHHGHE3FGGGGHHHHHFHHHHHHHHHHHHGGGGGHHFHFCGFHHGGGGGGGHHGGGGHHGGGGGGHHHHGGGADCHHHHGDGGGGGGGGC@DGFHEHHHHHHGHHHHGFGDEGFGFFGGGGCFGBEGFGGGFG;C.BFFFFFFFBFBFFFF/F?.AFFF/BFFEFFFFFEFF
-@M01368:8:000000000-A3GHV:1:1104:7756:4776/1
-GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTG
-+
-CCCCCCFFFFFFGGGGGGGEEFHHHHGHGHHHHHHGGHHHGGGGGGHHGHHHHHGHHGHHHGGFHHHHHHHHGHHHFHHGHHGFGGGGHHHHHHHHGHGHFFHFHHHHHHGGHGFGFHHHGHG?FGHHHHHHHHHHHHHHGHGHHGHGGCDGFFHHHHGHHHHHGHHHGFHGHFHGHGHGH/@CABFFFFFFFACAFF?BBFFBFD-@-.D>FFAF.F.:9---;AADFFFFFBEFFF:9FFFFBBFFFF
-@M01368:8:000000000-A3GHV:1:1105:11310:21761/1
-ATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTT
-+
-CCCCBFFFFFFFGGGGGGGGGGGHHHHHHHHHHHHGHHHHHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHGHHHGHHHGHGHHGHHHHGGHGGG=FFGEHHHHHGHFGGHHGHHHHHFHHHHHHHGGHHHHHHHGHEHHGHHHHHGGGGF9AGE?DEGFFGGGGGGGGGGGGGGFFFF/9FFBFFFFFFFEFFFBFFFFFFFFFFFFEFFF
-@M01368:8:000000000-A3GHV:1:1105:11578:14676/1
-ATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGT
-+
-CDDDDFFFBFFCGGGGGGGGGGHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHFGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHHHHHHHHHGHHHHGGGGGGHGGHGHHHHHHH;CHHHHHHHHHHHHHHHGHGGGGFGGGFGGGGGGGGGGGGFGGGEGGFFFFFFFFFFFFFFFFFFFFFFFFF/FFFFFFFFFFFFFFFF
-@M01368:8:000000000-A3GHV:1:1105:13575:19255/1
-TTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGTTCCCGTGAGTGGTTAATAGGGTGATAGGCCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG
-+
-AA1A1DFFFFFBGG3A111FFAEGDDBGFDHF00F01BBA00BFGEHFBFFA1FABB0FAE?CFHF1BGFHFB0011F121E//>>1F10>B?EFHGCC/?/BFG/EEGFFEE//BCFDE0?E@@B/?11?//<@?/F<<AAFFFFFEAGGGFGGAA6FHDGGCGFHFFHFHFHHHHHGDDDFFFHGFFHGBEGEGHHHFFFHGEEAEGHHHHHFHHHFHGHFEDGGEGBEAGEFHHFFHHHHHHGGHHHGHHGBGHHFHHFGHHHHG?CG?AFHH1FHHHGHFHHHF?DGHFFHCGAGEFHGCBBBB?BFFF?B-B.9/BFFFFFB=-AD?..@FFFF@@-@@?AFD.FFFBBEEBD9/:FFFFFBB/:FF9BF/BF.BBFF/
-@M01368:8:000000000-A3GHV:1:1105:21099:2895/1
-TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATT
-+
-CBCBBFFFFFFFGGGGGGGGGGGGGFHHHHFHGHHHHHHFHHHHFGFHHHHHHGHHHHHHGHHEHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHHHHHHHFHHHHGHGHHHGGHHHHHGGHFFGHGCGGGHGHHGHHHFGHHFHH/FGGGHFGFEGGCFCCD;B@DFFFFFFFFFFFFFFFFFFFFFFFFF;F.BEFADFFFF.AEFFFFFFE;D.;:B00099:09BFBFFBFF.0:000BFFBF
-@M01368:8:000000000-A3GHV:1:1105:26469:19245/1
-TTATGATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCAAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATG
-+
-BAAAAFFFBFFFGGGGGGFFGGHHHHHHHHHHHHGGHHGHHHGGHHHHHHHHHHHDEGGHGHHHHHGEDHFHGFHGGHHGGHGGEHHHGHHGHHGHHHGGEGGGHGHGHHHHHHGHHHHGHGGGGGHFHGGGGHHGGGGCDGHHHGD>A@FFFFFFFGGGFEECFGGFFB5GBHH5DFH5GBEEAEFHFHHF3F3GHFHHHEF3GAEHBGFHFFEFFHHHHFFB31FGGEGFHGEHHHHHHHHHHHHHHHHHEEEEGHHHFFF3GF3FGF44BBGEHHHHGG2B/BGGHE;B=.9.;.BBFFF..;A;/B/;B//;;//BBF
-@M01368:8:000000000-A3GHV:1:1106:22927:3863/1
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGACTT
-+
-A>ABAFA?D@AFGGGGFGGGFGFHHHFFH4BCBGHHHHGFFF3G5BF3322EEAFHHHHHHAB5GHFHEHFHHH3BEEHFGF5@13FDF3GDGFHHFGHHHGHHHGFGGHHFCG//?/EGHHHG3F4BFFGHHHEDGFHHB/B/?FBF<@-9?BDEFFF?B--?DFFFB9FA>B;A.BFDB?AB.--@;-.FFAEFFFF9AE;DDFFFFB/9BF
-@M01368:8:000000000-A3GHV:1:1106:23625:4236/1
-TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATT
-+
-@?AAAFFBFBFFFGGFGFGGGEFCGGHHHHFGHGHFHGHHHHGGFGGFGHHGHGHHHHGHGFFHHGGGGGHHHHHHHHHBFHHEFHHHHHHGF?FAGHGFHHHGDGFHHGGGHGHFHHHGHHHGHHFHHGFFGFHHHGHHHHFFHHHHH/FGHHHHGHGHGBFFF9
-@M01368:8:000000000-A3GHV:1:1107:14451:26748/1
-GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGAC
-+
-BABBBFFDABBBGGGGGGGGGGHGHHHHGAGHGGGGGHHHHHHHHGHHHHHGGGGGGFFHHHHHHHHHFHHHHHGGGGCHFHH3FEHHGCGGGDGHHHGFGGHHGGGGHHHHHHG/DGGGHHHHGGGGGCCHGG?CGG1GHHGHHHHBGHHHFHHGHHHHGGADAD00ECEGHEFHHFFAEFFFFFGGFBFBFFFGFFG.;B/99;BCFGGGGEFFFFFFEDA./:ADEB?FBFFFF;FFEBFBFF
-@M01368:8:000000000-A3GHV:1:1107:18714:24788/1
-GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACC
-+
-BBBCCFFFDEDEGGGGGGGGGGHHHHHHGGHHGGHGGHHHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHCFGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHGHHHHHHHHHHHGHHHHGGEGEGCCEGGHHHHHHG:CAFFGGGGGGBFEFGGGGHGAFFGGFGGGGGGGEFFFFFFFFFEF/EDDFFFFFFFFFBFFFFFBBFF
-@M01368:8:000000000-A3GHV:1:1107:21355:8333/1
-ATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATG
-+
-ABCBBFFFFFAFGGGGGGGGGGHHHHHHHHFHHHHHGGGGGHHHHHHFHHHGHHHHHHHGGGGGHHHHFFGHGHGGGGGGGHGGEGGGHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHGHHHFHHHHHHHHGHFFHHHHGGHGEGHHGHGFHHFHGHCEFHEGHGHHHGHHHGGGGGGCFFFFBFGGFGGGGFBG?FDGGGEGFFFFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFF.AA/9
-@M01368:8:000000000-A3GHV:1:1107:22864:15712/1
-AATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTAC
-+
-BBBBBFBBFFFFGGGGCEGGGGHGHHDGFHHHHHHHHHHHHHCHHGHFGBGDFGE?GHHGHFBHHFHHHHHHHHHGHFGFGHGHHGGC@DCFGHHGGGGGGGHHHHHHGGGGGFGGGGFFGGGGFADGFEFFFFFFFFFFF9;FFFF/BFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFEAECDFFFFFFFFFBFFFFF
-@M01368:8:000000000-A3GHV:1:1107:24817:14233/1
-CTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGA
-+
-3AABABFFBFFFGCFGGGGGGGHHHFGCFGGGFFGHHCGHHHGHGHGHBFFHHHEDGGFGFGHHFFFHGGEGHFHHGHFHGFFHHHFGHHHFHACECEHHHBGFHGFHHHFHHF4GFHFHFHEHGGE/EG:A-CFGGFGADGGGGFGGGGDAAAADFA.-AFFECFDAAFFFFEFFFFBFF;D;BFFFB/;BFFFFFFFFFFFFFFBFFBF/;//;BFFFF?FFFFFFEDAFFFFFBF//:BFBACF
-@M01368:8:000000000-A3GHV:1:1107:24830:14440/1
-AACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCA
-+
-ABBBA@FFFFBBGGGGGGGGGGHGHHGHHGHHHGGHHHHHGHGHHGDHHHHHHAGGGGFHHGHHHHHHGHHHHHHHHHHHEGAFGFHFHHHHGEGHHHHHHHHHHHHHHHHHGGHHHGEFEGGHHGHHHHHGGHGFHH/?GHHHHGFGHGFHCGGBBDAFFFEFFFFFFFFFFFFFF=CDDADDFFFFEF=BFFFEFFFBFFFFEEBF./:FFFFFFFFFFFFFFFFFFFEFFFFFFFFFFF/BFBEFF
-@M01368:8:000000000-A3GHV:1:1107:26478:23602/1
-GCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGAT
-+
-1AAAAF1B13BBBEGGGFGGGGGGHHDB1BGFHHHFGFGGG0F00BGBGFGGF1GBGB1BGCGAEE/FGFHGHFHHHHHBFF1B0FGGF//F//FGFGFFF/1@B1DFHHHGGEFEDHFDFAFF0B//1FDD1GFFHHDF?1BD>GF/GCAC0C@?@-;99BBB->@--;-B//99B--9-BF=A--A-:A----9AA9AFBFBBB?E@BBFFFFFFFBFFFFF
-@M01368:8:000000000-A3GHV:1:1108:11059:23181/1
-GTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGA
-+
-CCCCCFFFFFFFGGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHGHHGGGGHIIIHHHHHHHGHHHHGHHHGGHGGHHHHHHHHHHHHHHGGGGGHHGHHHHHHHHHHHHHHHGGGGGHHHHHFGHHHGGGGGGHHHGGGGGHGGGGHHHHHHGGGGGGHGGHEFGGGGGHGGG-ECHHHGGGGGGGGGFGFFGGGGFGGGCF?EFGG0;BFFFFB?9AAFFFFFFBF;BBFFBFFFFE?F
-@M01368:8:000000000-A3GHV:1:1108:12233:13498/1
-TAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACC
-+
-BCCBAFFBCCCCGGGGGGGGGGHHHHGGGGGHHHHHHGHGHBGFHHHHHGGGGGHHGGBFGHHGGGGGGGHHHGGGGHHGGGFHHHHHHGGGGGGHGHHGGGGGGGGGGGGGGHHHHFHHHGHGHHHHHHGHGHHGGFGGGDFFGGHHHGHGH/FHHHFHGHGHHGHHHGHHBAAAA3BDFFFFDGBFFEEGGGGHHGHFHHCFHGHGHHHGF3BFGFHGFEFHFHGH3EGFC1EEGBFGBHFGGFFHHHGHFHGHHGHGGHCEHEBCFF3ED3@GH4FDEG12FFHFFHG/G3FGA/@EFEFCG33FFGBBGHFHH/?BFBGFGFAGC2C/@-;@-;BFFEFADF?DFFFFFF?;AFFA;AD;..FEFFA--;9;D.9BFFBE?;.;///://///99//BB/9;F./B//BBFB9//
-@M01368:8:000000000-A3GHV:1:1109:25237:11722/1
-CCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATG
-+
-AABBBFFFFFBBF5BGGFGABGHGGAEFGFHHHHFHCHFHHBFGGGEHBGGHFFFFGFAEF5FHH?EGGF5AEG3?EBFHD?C/EFHFFEGEE?CFGGGHHFHB3EEFG?EEGHCFGGGGGGGECA?FFFDFBFGCG4EGFG??GACFGGHFHCFHCHBECDEFCEGHHHHHFFGGF3AFFHHFGEGCGHHGEHGFGHHEGG?EFHHH?EGGHHGGGGHHHHHHEGGGGGHFHHGFDDGGACFCC@?/FDDGHHHGHEFFHHH0FDHHFFEDCD.CFHHGDFGGGGGEFGC?EGHHHB0CGHHGCGHHHHBEHGHH.::ACA;FECFGGGGGFFFEFGGGGGGGFFBBBFGBFFFEFFFFBFFFFFFBBFBFFB9..AF?FFFFF
-@M01368:8:000000000-A3GHV:1:1110:14751:26540/1
-TAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACTGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGTTGTGCACGCGATAGCATTGCGCGACGCTGGCGCCGGCGCACCCTATGTCGCAGTATCTGTCTTTGATT
-+
-AAA?1CFFFFADGEEGFGB1112DGDGHGFGHHCG/ECEBGGHDFGHHFHGHEB1BCGFFE2EGHHGHFFFHHBFFHEFCGGHHFHHHHH111BFFHGHEHBEHGGECEFHGFHFGGDBBGHHBHHHH0GH22FGHGFFFH1BFCG0F@FGG11@GDHFG1D/1FGFHH/FCG.>0><-<@CCFFG.CC-@C?C9FFF0C---;------;9----9-9@-AAFF9B9---9-/;B9BF/9BB//B/
-@M01368:8:000000000-A3GHV:1:1110:15519:10008/1
-CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAAC
-+
-BBBBBFFFFFFFGGGGGGCBGGE?GGGHHHHHGHHBEGGHHGGGGGHHHHHHHHHHHGGHHHHGFGGGGHGHGCFGHHHGGGCGGHGHGGGGGHFGGGHHHHHHGCEFGGHHHHGGGGGGGHGG/CEGHHHHGFHHHGFHHGFFHGHGHFGGEGFDDGGBCGHHGFHH/CHHHHGHGHF0CFGGGGGGGFFFFFBFFFFFFGFFGGF?DGEDEEFEFFFF?FFFFFFFFFFFFFFFBFFF.BFFFBFBBA
-@M01368:8:000000000-A3GHV:1:1110:16559:7507/1
-CATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAACTGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGAT
-+
-ABBBAFFFFFFFFGGGGGGGFGGGGAGHHGCHHGHHHHHHHHHHHGHHGHHHHHHHHHHHHHHHHFGGGHHHHHHHHBDHHHHHGGHFEEGHGGHHHHHHHGHHHEHFGGGGGGHHHF33FGHHG3GDDGH//EEGHHFFBF?GHHFCC@DGFCGGB?DGHAFGGHHGFH0E??<@DGHGGCFGGF?DGGGFFGGGGGGGG.C/0BBF?FBBEGG0FFF.AA9;B9/E////99;;:.//:FFFFBBF//
-@M01368:8:000000000-A3GHV:1:1110:16700:6843/1
-AAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAAC
-+
-BBBBBFFFFFFFGGGGGGGGGGHHHHGHHHHHHHHHHHHGGGGGHHHHHGGHGHGGGGGGHHHGFFGHHGGGGHHHHHHCGG@FGHFHFEGGGGGGHGGGGDGHHHHHHHHHHHHHHGHHGHGHHGGGGGCGHEHGHHHFHHHFF.DGHHHHHHGHHHHHHGFGHGCGHGHHHHHHHHGHHHCD?:A::FCDGEGFGFFBFGBFGGGF00CBFFFF/BFBFFFFA
-@M01368:8:000000000-A3GHV:1:1110:18014:7856/1
-ATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATG
-+
-AABBBFFFFFFFCGGGCGGGGGGHHHHHGFHHHHHHGHHHGGGGGFHHGHFGGHHHGGFGGGHHGGGGGHHGGGGHGGHHHGGGGGGHHGHGDGGGGGGGGB/ACC=@--AFBFFB?=BFDFFF//FDB;-9A-AD-.A.A=?-9-:B/AEB/BFF.A;DDFFFBBFFFF/BBFFFFBFF9B
-@M01368:8:000000000-A3GHV:1:1111:13195:14562/1
-ATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATAC
-+
-ABAAAFFFFFFFGGGGGGGGGGGHHGG4FDFGFGGCGGGHGHG?EGHEGFFDHFHHHHGGFE@EHHGEEEGFCCEFFGFG/EHHHHHBFBGGHFHFHEHGHEFGGGED/EFDEFFEHHBGHHCFFFDFGGHHGFFFHHHHGFG>0<0DGGFHBGHFBDFGHGFFGGAGHHGCGBHFFHHHFFFBFGGGGF/:FFFCB9F9FF0FEFE?FF0BED-D./
-@M01368:8:000000000-A3GHV:1:1111:14287:20817/1
-GCCAACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTCATCGCACCTACGTTCACTATTACAGGCGAACATACTTATTAAAGTGTGTTAATTAAT
-+
->>A3>AAAACFFFGFGFGGGGGHGHHHHHHHGGGGGHGHFFCGHHGBGHFHHHHAGHHHHEHHAGFFEHEAE?GHHHHEGFFFDGHGHBFFHFHHHGHGHGHBECG@@@EHGGEGCGGGGFFGGGG?BGGGGGG.-C.FEDCFFFEFFFAF/99BEFA.:9BFB/9FFFEFFFFFFEFFF/FBBFFFFFFFFFFFB//;.@@//;DFFFF//BBFFFBF;A;DF=>B//;9B//B/;/;9//9//;B/:B9
-@M01368:8:000000000-A3GHV:1:1111:23624:14516/1
-GTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGGGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTA
-+
-3>3AAFFFFFAFGGGGGGGGBGHHHHHHGGHGGHEHGHADFHHHHEGGGHBGHFFHHEHHGHFHGG5FBEEGEGHHHGHHHEFDGHHFFGFGGHHGHHHGHBHGHFHFHHGGGGFCFBGFGE?GFBECEGDGHEHGDGCGHGC/DF@GHGFFCGGGGFFFFDD?@C@GFEC?-ADHFFHBGHFBHFG0CC//.;B0/CA.;AA.;@.AB/9BB/A/.AFFFE/BFFF///9B/FBDE9/;99/B/BFFEF
-@M01368:8:000000000-A3GHV:1:1111:7437:24508/1
-ACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGT
-+
-CBCCDCFFFFFFGGGGGGGGGGGGGGHHHHHHHHHHHHHHHHGGGFFEHGGHHHHHHHHHHHGHHGGGGGHHHHHHFHHHHGHHHHGHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHGHHGGGGGGGHGGGGGCGHHHHGHHGHHHHHHHEDHHGHGGGGGD;BEGGGFFGGGGC:CFGGGGGGGGGFGGGFFFGFFFFF;
-@M01368:8:000000000-A3GHV:1:1111:8665:25542/1
-TCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTGTCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGG
-+
-AA3AAFFFFFFFGGGFGFFGGGGHGH4AGGGFHHCGEE2FGHHHHHHHHHHGFHGF53FHEGGFAF5CFHHGGFGHFHHGGHHHCHHHHHHHFHHGH?EEEG1FFFEHHFHFGGHHGG?F4FBGHGHCHEF0//-9--;0CFGG?@BGDGFFFFEFBBBDA?FFBFFFF=B;-9.AA9EFFFF/;9BD.//;9/9/B:/:BFBFFBFFF/B:BFF//;/B/FFFBFF.A-BFFFBA/F//BFFFBF/;/
-@M01368:8:000000000-A3GHV:1:1111:9655:13916/1
-GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGCGGTGTCCACGCAATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTT
-+
-1>>1AAFFFFFBGGFE1EEEEGGHGHHH2FGHHHFGFGHGCFGGGEFHGGEGGHHHHGHEFBFEGHHHGHHBEHHHHGHGFHHHGGGGHHEGGGGHHHGFHGHHHH1FHHG?G/AGF1GBFGEFFHF2FDD0FG--99@=---AAABA-BF/B?A@F-9BBFF//BB
-@M01368:8:000000000-A3GHV:1:1112:10272:7828/1
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCAC
-+
-AAABABBBBBBFGGGGGGGGGGHGHHGHGHHHHHHHHHGHHHGGHDGHHHGGGGHHHHHHHHHHHHHHHFHGHHHHGGHGGHHHFHHHHHCGHFFHHHHHHHHHHHHHGHGEFGAEEEEHHGHHGHGHHHHHHHGHHHHHHGHGHFFBC/BA?BGGGFFFFFFFFFFFFFFFC-@DDFFFFFFFEFAFFFFF
-@M01368:8:000000000-A3GHV:1:1112:9832:16531/1
-CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGGGCTGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCTTGAAGTAGG
-+
-AABABFFFFFFFF4GGGGEBGEFGHGGHGHGHHHGGHFBGFGGGGGHHEFHGEHFHFHEHHHHHGCGGGHFHHCFGHE@1EEEFFGB?EEEEHBGGGCHHGHHBEE@>EAHHFAEBDGGGCEFF?CEGFFHHHHHFHHHFHGHCGCAHHF<..C.D=0CD.GFHHHG.CAGHC:CHB0GGGFFB0:CFA.A-;9.;000CBBFFBFGA.9AA;A;EDDE.ABBFFFF9;BEFFF/BF//9/B//9:B
-@M01368:8:000000000-A3GHV:1:1112:9832:6701/1
-CGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGG
-+
-AABBBBFFFFFFGGGFGGGGGGHHHGGHGGHHHHHHHHHHHGHHGGGGGHHHHHHHHHHHHHHHHHHGGCGDHHHHFGGHHHGGGGGFGGHGGGGHHGGGGHHHHHHGGGGGGHHGGGGDGGFGHGGFCDCHHHHGHHHHGHHFAGFGGEHFGEG.C1>11F1D1DDFFFFGGG1FH3GHGHGHGHFHEC?EC0B1FBGFB1GHHH21AF2HH2FEGEHAE10AAE/>/FFHE?/E/?E1@EFFH?E@/EGGG//B11B//<@@C0F/<0AAAAFFFBFFFGGGGGGGGGGHHFHGHHHHGHHHHHHHHHHHGGGGHHHHFHHHHFHGHHHHGAFFEFHEGHHHHHHHHGHEHHGGFGGGHHHHHHFHHHHHGGHHHHGGGGGHFHHFF?HHGGFECEFFGHFFGFHGECDGHGBGFHGDF@@?CGFHCEGGGFD.CCC?EGHBHHHFHHFBCFFGEB/CEGGGGDAA.90C9CEBFGGBBF/9.9FBFFFBBFF//99FFFFEABF//99FFEFFFBFF
-@M01368:8:000000000-A3GHV:1:1113:5741:16959/1
-TAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTAT
-+
-ABBBBFFFFFFFGGGFGGGGGGHHHGHHGGHBGHGAGFFCAFGHGFFGHHGFHHHHHGGGGGHGHHHHHHHHE3BFFHHHGG0EDF@GHFFGGGHGGGGGGGGGGGGGHHGGEEFHGFHHDDG@DGGGHHGDGGGGGHGG?CF?HHGHHHGHGHHHFFHGGGHHHHGGCD.;0A33>FFFFFF@FFFGGGGFGGD4EGGGGGHHGFFGHFGGHHHFEFHECFHHHEHGAGBCCGCCEGGHGHEGFBFHFHHHHGGGHFHGHEGGGFEGEGG??@DBGHGGC-@FFGHFHFHB-??DA-DD@9:BEBG@B?E?A.ABBFBFA??F.FF/BFFB/B9B/9
-@M01368:8:000000000-A3GHV:1:1114:14540:5315/1
-CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGGGGGCTATTTAGGTTTT
-+
-AABCCFFFFFFFGGGGGGGGGGHHHHHHHFHHHHGHHGHHGGGHGGHHHHHHHGHHHHHHGGGGGHHFHHHFGHHGGFHHHHHGGGGGHHHGHGGHHHGGGGGGHGHGGGGHHGGGGHHHHHEGDDFGFFFHHGGGGGCDAFCFGFDHHHHGGHGHHHHHHBCGEHHHHGGHG.ACGEHGG0CBFFF:A;BB0;09CGF00CFFFE0AA?//CFFFFFFFFFFFFFFFBEF;A.-=A--:BBFB90;;FE
-@M01368:8:000000000-A3GHV:1:1114:15066:16302/1
-TAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAACGGTTGTTAATTAATTATT
-+
-BBBBAFFBDFBFBGGGGGFGGGBGFFFHGFHHGFFFHGHHHGHHHHFFHHHGHGC?AEFFHEFBFFFGHHHHH1FDGFFHGHGHFEGCGC-<>ABFFFFFFGGCGC1BBF1GHHHBHHHHGFHGH1A100AA/GGEHGFBAA1FFD2FHHHHECG2F1BB/E0FC/A>EE/FGGFGEF1EGBBEHHCGGGHBGEHFHE0B?E--;C?CCGGG@A@GBFBBBB09?@??A-AB--ABE@=-=-9AE9A;FFFFFE=9-//;//;9FF/////;;///9B///;/B////9FFBB;--A@-;/9;9-:-/;;FFFE9BF?@;-9-99/B9F/://///99/;
-@M01368:8:000000000-A3GHV:1:1114:2404:13066/1
-TCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCAT
-+
-CCCCCFFFFCFFGGGGGGGGGGHHHHHGHHHHHHHHHFFHHHHHGGGGHHHHHHHHFHHHHHHFGGHHGGHGGHHHHHHGHHFHHHHGGGGGGHHHHHHGHHHHHHHHHHGGGGGGGHH?FGHHHGGGGGGHHGGFGGHHGGGGHHHHHFGGGGFGHGHHGGGGGGGHGGGEGGHHGHHHHHHHHHGFBFFDA0FGGGFFGG0:EFGGGGGGGG;AEBF0B0BFFBFFFFFFF
diff --git a/tools/trim_galore/test-data/paired_example_pair2_results2.fastq.gz b/tools/trim_galore/test-data/paired_example_pair2_results2.fastq.gz
index 321f0c6d55..6f0f7d8782 100644
Binary files a/tools/trim_galore/test-data/paired_example_pair2_results2.fastq.gz and b/tools/trim_galore/test-data/paired_example_pair2_results2.fastq.gz differ
diff --git a/tools/trim_galore/test-data/paired_example_pair2_results2.fastqsanger b/tools/trim_galore/test-data/paired_example_pair2_results2.fastqsanger
deleted file mode 100644
index 2e31e60478..0000000000
--- a/tools/trim_galore/test-data/paired_example_pair2_results2.fastqsanger
+++ /dev/null
@@ -1,392 +0,0 @@
-@M01368:8:000000000-A3GHV:1:1101:14518:9998/2
-CATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATA
-+
-CCCCCFCCCCCFGGGGGGGGGGHHHHHHHHHHHHHHHHGFHHHHGGGGGHGFHHHHHHHHHHHHHHHHHHHGHGGEHGGGGCGGGHHGGCGGGGGHHGHHHGGGGGGGG.BFFFGAGADFGAFDGFGGCFFF;DDFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFBFFFFFFFFFFFFFFFFFFF09FFFE00;BE@;DABBFFFFFBBFB00;F:9;FFBFFF9BFFFFFFFFFFFFF90/::BFFFBF
-@M01368:8:000000000-A3GHV:1:1101:18422:19051/2
-CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG
-+
-BCCCCFFCFFFFGGGGGGGGGGHHHGHHHHHHHHHHHHHHHHGGGGHHHHHHHHEHHHHHHHGGHHGGHGGHHHHHHHGHGGHHHGGGGGHGHHHHGGGHFHFHHHHHGGGGGHBFFCGDHHHGGGGGGHGGGGGGHHGCGGGFGHHBGGGGGFFFHEGGGGGCDCCE@EFGHHHHFHEGHGFFHHGB;ECBFGGGEFEFFGF0AFGFGFFG.;;DFFFFFFFFFF090BFFFE?FEFBBFBFFFB990BF
-@M01368:8:000000000-A3GHV:1:1101:25545:21098/2
-GCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTTCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCAACCTATGTCGCAGTATCTGTCTTTGAT
-+
-3AAA34BFFFFFGCGGCGGGCGHHHGHHHHCGHFGHFHGFGGCDGGBD5FGFFFHFHGGGHAEBFGHHHEHGGFHBGGFHHHGGGEEFHHHFHFFHDHGHGHHHHFFHHGGFAEGFFFFFBGHHHFFHHHHHHHFHFHHFHGFHGHGA/<@EFHEHH1GGHFFFHG1>=FGHFHGF.GE//-AADEBGGGGFFCGGHHA5DFGAE?AAEDFHG2AEE1FBE00A1BGE0?E1FFGEEEGB4F43?EE/>///F??//@BCBAC<<.<<AABBFFCFFFGGGFGGGGGGGGHHHHHHHHHHHHHHHHHHGHHHGGEGGFFHHHHHHGHGHGHHHHHEHFHGHHHHEFHHGHGGGGGHHHHGHHHHHHHHHGFGHHHHHGHGHGHHGGGCCGGGGFGGGGGGDGGGGFGDGGGFFFFFFEFFFFFFFBAF:9AF//;BEF-DD/FFFFBFFFFFFFFFEBFF?FB;/;//BFFEFFE/::..9;-:B9:FBFFFFFF
-@M01368:8:000000000-A3GHV:1:1102:17584:10050/2
-GTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGAT
-+
-ABCCCFFFFFCFGGGGGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHFHGGHGGHHHHHHHHHHHHHHGHHHGGHHHHHHHGGHHHHGGGGGHHHHHHHHHHHHHHGGHHHHHHGHGHGGHGGFGGGGGGGGGGFGGFGGGGGGGGGFFFFFFFFFFEFFCFAAFEFEFFFFFFFCFDFFFFFFEFFFFFF
-@M01368:8:000000000-A3GHV:1:1102:18842:24844/2
-CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG
-+
-CCCCBFFBFFFFGGGGGGGGGGHHHGHHHHHHHHHHHHHHHHGGGGHHHHHHHHFHHHHHGHGHHHGGHGGHHHHGHHHHHHGHHGGGGGHFHHHFGHHHHHHHHHHHGGGGGHHHGFECGGHGGGGGGHHHGGGGHHGGGGHHHHHEFDCADGHHHHGGGGFC?AE-@DDFHFFHHHCFHGGFFGFFFGGGEFEGFGED9C?CEGF90FEF/A./BBF9FFFFFFFFBFFF.9AF////;FFBFFB/:BF
-@M01368:8:000000000-A3GHV:1:1102:21788:11027/2
-AATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGC
-+
-CDDDDFFFFDDFGGGGGGGGGGHHHHHGGGGHHHHHHHHGHHHHHHHGGHHGEHGGHHHHHHHHHHHHHHGGGGEHHHHHHHHHHFFFFFHHHGGGGGHFHGBFEHHHGGGGGGGHHGGGGHGGGGGHHFHHHGGG@CGHHHGGGGGGGGGGGEGDGFFDHHGEHHBCFHGBGFAGGHFGGCEEF0BE///FDFACF?FHFGC//??1B0</@GFGD/@1FG10FF>DFCFDFFFFFFFFF-BBAFFFFFFFFE@D9B;FBFFFFF
-@M01368:8:000000000-A3GHV:1:1103:25465:20010/2
-AACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGTGCACCCTATGTC
-+
-AAABBFFFFFDFGGGGGGGGGGHHHHHHGHHHGFHHHHHHHGGGGGHHHHHGHHHHHHHHEHHHHHGHGGHHGGGHHHHGGGGGHHHHHEHFGEHHHEE14BGHHHGHGHFCE/EFGGGGGGHGGGGGG?FFGGGG.ADGGGFFDA.FFEC--;9BBBD?FFFFF
-@M01368:8:000000000-A3GHV:1:1103:25519:6301/2
-CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATGCTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGGGATGTCTTATTT
-+
->AABBFFFFFFFFFFGGGCAFGCGHGGGHGGGHHFHHHFHHGGGGGFFFHHHBHHHHG5BG5GFEEEE1FGFFGF1EGHGGG/BFHEF@EA1BE1@EED3?4DHGGGGEGHFFFDDG?DCDEG@<?@DGCAGC-;@DGFFHGCHEEFGGFGFB/;..BBB.CCE?A0FE0EBFF0FEF.;=DFFB//BFBF//;/:9BA.9FB9//;9//;9F;FF
-@M01368:8:000000000-A3GHV:1:1104:13832:4398/2
-GACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTG
-+
-BBCCCFFFFFFFGGGGGGGGGGGGGHGGGGGGGHHHGFGGHHFGGGHGHHGHGFGECGHHHHGGGGGGGHEGFGGGHHGHFHHHHFGHFHHHHGHHHHFGGFGG3FFGGHHHHHHH2?FFGHFHHHHHHHHHBGHDGC0
-@M01368:8:000000000-A3GHV:1:1104:23670:4161/2
-CGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTT
-+
-A3AABBFFFFFFGGGGGGGGGGHHFHHHHHHGHGHHHHHGCHHHHGGGGHHHHHHHHHHHHHHHGHHGHGHGGHGGHGHGGHHGFGHHGHHHEHHGHHHHHGHEHEHHHGGFGGHHHFHHHGHHHHHHHGHHHHG4EGGGFCBBCA?DGGGGGGCCAFFFFFFFFFFFFFFFFFDFFF.9@--=AFFFF;FBFFBEFFF9FBFFB9/FFFFFFB/FFFFFFFBFFFF/BBFBB/FFFE?.;@FFB?FB
-@M01368:8:000000000-A3GHV:1:1104:24927:22253/2
-GTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGGGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGGGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAA
-+
-AABBBFFFFFFFGAFGG5GGGGHDGHHHHHHHHHHHHFHCFGC2F3BFGFGHBGHHFHEDHFGGGGGHHHG3F?GHHGGC/EFEHFGAEFFHGGAEHGGGG3EEGEEDHFHAAD@DGGGHG/AC1>GED-<<-.;C0:0C:C;GH:::::0B90;FFGEGFBFF0C.;9F0;0090;900;00FA9D.A...-9=BBFFFE/FE9;9E//BBBB.BBB///B/9/B
-@M01368:8:000000000-A3GHV:1:1104:4705:7727/2
-TCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCG
-+
-ABBBAFFFFFFFGGFGGFGGGGGHHHHFHHHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHEDEFGHFHHHHGHGHHHHHHHGHHHHHHHHFHGHHHHHGGGGGHHHGHHHHHHHHHHHGHHHHFHGHGHGGHGGGDCD.;FFGGGGGGGGFFFFFFFFFFFFF.>FFFFFF-BBEFFFFFBFFFFF-DDFFFFFFEFFFFFFFFFFFF?F///9FFFFF/;FFFFF?AADCFFEFAFFEFFFFFB/;B/A9
-@M01368:8:000000000-A3GHV:1:1104:7756:4776/2
-CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG
-+
-BBBBBFFBFFFFGGGGGGGGGFFHHGHHGHHGGHGHHGFHHGGGGGHHHHHHHHGFF3GGGHGGGHFDHEGHHFHHHHGFHBCGHGEGFFFFFHHHHHGHHHHHGHHHGGGGGHHGHHGGHHHGGGGGGHHECCFDHHADGGHHHFGFDC////FHHHDGFCGCDEECF?EGGFFHHFEHFGGHHFFF/A900;C.9..C0990.;CFBFGE/9.9B99/;B.99/FB///9AEF/99/9/BBF/B/B//A
-@M01368:8:000000000-A3GHV:1:1105:11310:21761/2
-ACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCTC
-+
-BCCCCFCCCCCCGGGGGGGGGGHHHHHHHHGGGGGHHHHHHHHGHHHHHHHHHGHHHHHHHHGHHHHHHHHHHGGGGGHHHHHHHHHHGHHHHHHHHHHHGGHGGHHHHGHHHDGHHHHHHHHHHHHHHHHHGHHHHHGGGGGGHHHHHGHHHHHHHHFFHHHHHCCAHFHHGGFFFFFFFFFAA;BDDFFFF00.-=D.ABDA-.::AAF;F@.AAD./0000.9-;.9:00009:F090;FF000900
-@M01368:8:000000000-A3GHV:1:1105:11578:14676/2
-GCTCCGGGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTAT
-+
-CCBCCCBBCCCCGGGGGGGGGGHGGGGGHHHHHHHGHHHHGHHHHHFFFGGHGHHHHHHHHHHHHHHHHFGHHHHHHHGHHGHFHFGHHHGGGGHHHHHHHHHHHHHHFHHHHHHHGGHGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHDGGCGHHHHHHHHHHHHHFF>ABFDCFFFFFDGFGFDGDEG1EGEFFBFHFGHA0E00A0CAAC0FFDFFAGHFEHHFDH/EAFCFFHACGHHAFFEF2DFFG/FGHHHHGHGH0C@/E@GGC0FG10>GGFGHHGHFFBGFGB2>F@;-A@FF<-;---@-;-----;-BFF/;---9//BBFFFF//B;FF///9BF///;/;B/BBFBFFBB/;-9--///;9/:-;BFFBBB9F
-@M01368:8:000000000-A3GHV:1:1105:15502:11453/2
-GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGAC
-+
-CCCCCFFFCFFFGGGGGGGGGGHHHHHHGHHHHHGGGGGFHFHHHHHHGHHHHHGHHHHHHGHFHGHGGGGGGGGHHHGGGGGGGGHHHGHGGGGGGGGGGGHHGGGFGGGFEGGGGFFFGGFGDGGGBFGFFFFFFFFFFFFFE0BFFFFFFFFFFFFFFFFFFF;BFFDFFFFBF0BFFBFFEEBFFAA009FFFFFBFFEBB0;.BFFBFFFFFFFFFFFF0;BFFFF
-@M01368:8:000000000-A3GHV:1:1105:16787:9771/2
-TCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAG
-+
-CCCDCFFFFFFFGGGGGGGGGGGHHHHHHHHHHHGGGGHHHHHHHHHHHHHHFHHHHHHHGGFGGHHEHHHHHAGHHGHBHHHGHHHHHHGHGHGHHHGGGGGHHHHHHHHHHHHHHHFFHHHHHGHGHGHHGGGACAFGGGGGG-AFGGFFFFFFFFFFFFFFFFFFFFF-9@AFEDFFFFFB.9-AABBBFFFEFFFFFFFFBFFFFFFEBB0BBBFFBFFBFBEDEAFFEB.ABFBFBFFFF0::
-@M01368:8:000000000-A3GHV:1:1105:17564:15723/2
-ATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTT
-+
-CCCCCCCCCFFFGGGGGGGGGGHHHHGHHGHHHHHHGHHHHHHFHHHHGGGGHHGHFHHHHHHHFHHHHHHHGHGGHGGHGHHHHFHFHHGHHHHHHHHHHHHHEHGHHHHHGGGGGHHHHGEHHGHHHHHHGHFGHFHGGAHGGHGGGCCBDFGFFAFDBDDFFFFFBDA;AAD-ADC?AFEFB-@@AAEFEFFFFF..-:.B0:FFFFFFEF0BFF09FF.0;9BFFFFF0BFFFFFF?.::-909;.9
-@M01368:8:000000000-A3GHV:1:1105:18108:12091/2
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTA
-+
-CBBCCFCCCCCFGGGGGGGGGGHHGHHHHFHHHHHHFHGHHHHFHFHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHGHHHHGHFHGHHGHHHHHHGHHHHHHGHHHHHGGGGGHHHHHHHHGHHHHHGFGHHHHHGHDHGHHGCA;?AAFGFEFDFFF.FFFFEBDFFFFFFFFBFFFFFFFFFEFEFFFEFFFFFFF.:BFFF0BFFFFFFFFFFFF;0FF0;BFFFFF
-@M01368:8:000000000-A3GHV:1:1105:18331:16683/2
-TTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGGGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATAT
-+
-CCCCCFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHGHHHHHFHGHHGHHHHHGGFGHHIIHHIHHHHHHHFHCFGHEEHFFHGHHFGHGHGHFHHGGGGGGEEGHHEHFHHGGHFHHFGGGGDFGBCGG?DGHGGFCGGHHECEF?CHGGDGFFGGHHC?EEGGHHHH.>AEGGGHEE-AEG0C0GCCEFFFFGEFG/;CEGGGE.:CAE.;@.@EFBFFFF/..:BFF0:BB00FBFFFFBDAFFF0;
-@M01368:8:000000000-A3GHV:1:1105:20577:18421/2
-ATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGCCGAAAATACCAAATGCATGGAGAGCTCCCGTGAATGGGTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATT
-+
-?ABBBFFFFFFF4CFFGGGFGGHHHHHHHCEGHHCHFGHCAFEFHHHGGAEGGHHDHHHBGHBGHHH?5BGE1AEEFHHHHHGHFHBGHGEGGGGHBFHHFGHBGCFFG4EGHGG?E/FG3F3F/FGCDECEA/FGG/CEDBB//?BHEHHF0ADC@D>D9@DDCFFFFFFCFDFE.AA;/BB/.;-.;BFBBBFFBFFFFFFF/9FF?/;/BBFFFFF/;FFB
-@M01368:8:000000000-A3GHV:1:1106:17389:18671/2
-GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTCTTTAGGGTTTATGACC
-+
-BBCCCFFFCBCCGGGGGGGGGGHGHGHHGFGFGFHGGHHGHHHHHHHHFHHGGGGAGFBGHHHFBBGH5GDHH5EEEGEHEGFBF1DGFDGFGGGBBCEEGEHFEFDDHFBFHFD/@BGDGDHHGDCCFCD/C<->.ADDGE1GA;9.FFFFFFD-99ADFFD-.9FADF--9..;AAFEFFEAA-;.///BB:BBF/BBB//B//99/;BBB/9////F
-@M01368:8:000000000-A3GHV:1:1107:18714:24788/2
-GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACC
-+
-BCCCCCFFFFFFGGGGGGGEGGHHHHGGFFHHHHHHHHHHGGGGGHHHHGHHHHHHHHHHHHHFHHHHHHHHGHHHHHHGHHHHGGCGHHHHHHHHHHHHHHHGHHHHHGDGHGGHHHHHHHHGHHHHHHHHHHHGHHHHHHEHHHGHFGGGGHHHHEHFHHGHFHHF.>GGHHHGHGHGGHCFFCDFDFFFFFFAAF.FFFFFEFAFCFFA..@.AFFFC9--9;.
-@M01368:8:000000000-A3GHV:1:1107:21355:8333/2
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCGGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTA
-+
-AABCCFCCCCCFGGGGGGGGGGHHHGHHHFHHHHHHGHGHHHHHHGHHHHGGGGHHHHHHHHHFFHHFHGHHHHHHGGHGGHHHGHHHHGGHFHHHHHHHFHHGHHGEFFHFHHGGGGGGHHHFHHHHGHHHHE?FGHHHHGFGHGHHGGGDGGGGGFFFCFFFFFFFFFFFFA;DBBDFFFAFFFF;DBAFEDFFFFFEA?-9...9;9;BFFFF//BFBFFFFBFBFFFFFB/BFFF9B/EDEF-BF
-@M01368:8:000000000-A3GHV:1:1107:22864:15712/2
-GTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATT
-+
-AABCCFFFFFFFGGGGGGGGGGGGGHHHHHHHHHHGHHHGGGGFHGHHHHGHFGFFFFGGHGGGGGCHFHFAFEDHHGGFGGGHHEGEGGHHEGGCHGHHHHGGGGGGHGF0EECGGGGFECCFFGFBGHHHHHHGGHHGHFCFEHFBEFGGADDDC=CC0CCFHG:CEHHHGHBGH:GFFGFF0CAAA/BF09BBBBB
-@M01368:8:000000000-A3GHV:1:1107:24817:14233/2
-CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAAAGTGTGGGCTATTTAGGTTT
-+
-CCCCBFFFFFFFGGGGGGGGGGFHHHHHHHHHHHHHHGHHHGEHGGHHGHHHHHGHHEHHGGGGEHGHHHGEHHGHHDGHHHHGGGGGFGEFGGEGHHGCEEEGHHEFGGDHGGGGAGFHHHHGGF/?//GHFFDGGGGGHAD??DC1DHHBGHHHHGHF</ACFHHCDCDE?CGEBEGFFFGF?.?DGGGGEFEFFB00CFFFB.:AFFB;/BB9BBFFBBFFFAD.;///9.A./A:FB;BF//B
-@M01368:8:000000000-A3GHV:1:1107:24830:14440/2
-CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTAGTTAGGTTTT
-+
-CBCCCFFFFFFFGDGGGGGGGGHHHHHHHFGHHHHHHGHHHGGHGGHHHHHHGHHHFHHHGGGGGHHHHHGHHHHHHHHHHHHGGGGGHGHHFGGHHHGGGCGGGHHGGG?GGFGGGHHFHGH?FG/CDHHFAAEGGG/DHFC?-.AFGFH0CHHFH0DHHG/CDEBFHG.CA.:0C:;.FGFEFFG:E.B909BFF/;CFGBB9BBA.:CFGFFFFFFFF/;//AF?.ADEAB.9.A/;.:BFFF/9FF
-@M01368:8:000000000-A3GHV:1:1107:26478:23602/2
-ATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGCGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTTAGC
-+
-AAA?AFFFFFF>FGGGGGGGGGGGFHHEFGHHHGGGGGGHHHCGGEFHFGGGFHHHHHGGGEGGHHFHEEGEG/EEEE/?/EHFHH3GGH4EGHHCB01CGHGHGD//<@CGGBFFDGHHHH0FEHHGEGFHCD11>0=GGHGCCFGHGBGGD0GGHC0C.@?ACEEC/.EDEB?BFFCFB9CFF090BBCBFBF0CCB0B00A.9E.B/9BA=-..B9FBF//;BBBBFF
-@M01368:8:000000000-A3GHV:1:1108:11059:23181/2
-AACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCAC
-+
-CCCCCFFFFFCCGGGGGGGGGGHHHHHHHHHHHGHHHHHHHHGHHHHHHHHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHHHHHHHFHHHHHHHHHHHHHHHHGHGHEHHGFCGGGHHHHHHHHHHHHHHHFGHHHHHGHGHGHHGGGBFGFFFFEAFFFA.BFFFEFDDFFFFFFFFFFEEFF;D;EFFFFFF0BFFFADDFFFFFBFBFFFFFBB0FFFF0::0BF000BFFFBBF0EDFF
-@M01368:8:000000000-A3GHV:1:1108:12233:13498/2
-GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTA
-+
-ABBBCFFFFFFFGGGGGGGGGGHHHGHHHHHHBGHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHGHHHHHGGHHGHHHHHHGHHHHHHHGGHHHHGGGGGHHHHHHHHHHHHHGHGHHHHHHGHGHGGHGGGGAGGFGGGGGGGGFFFFFFFFFFDFFFFDFFFFFFFFF>.AEDEFFFFF?E-DAB9FFFFFFFEF:FBFB009:0FFFF9BFFBFFFFBB0.9A=-/0
-@M01368:8:000000000-A3GHV:1:1108:14328:14751/2
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTAT
-+
-ABBBBFBBBBBFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHDHHHHGGGGHHHHHHHHHHHHGHHHHHHHHHGGHGGHGGHHHHHGHHHHHHHHHHHHHHHHFHGHHHHHGGGGGHHHHHGHGHHHHHHG?FHHHFHGHGHGHHGGGGGGGGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFAFFE.ADEFFFFFFFADFFFFF///BBFF/FFFFFBFAFFFFBB///;9:
-@M01368:8:000000000-A3GHV:1:1108:16350:22621/2
-CATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATAT
-+
-CCCCCFFFFFFFGGGGGCGGGGHHHHHHHHGHHHHGHGHHHHGGGGHHHHHHHHHHHIHHHGHHHHHHGGHGGHHHHGHGHGHHHHHHHHHHHGHHHHHHGGHHHHGGGGGHHHHHHHHHHHHHGHGHHHGHHGGGGGHHGGGDADDGFFGGGGFFFFFFFFFDFFFDFFFFFFFFFFFAFAEFEDFFFFFFEE-AAFFFFFBFFFFF0FFFBFF;AFFFFFFFFFFFFFFFFFFFFFAB9BFFFFFBFFF
-@M01368:8:000000000-A3GHV:1:1108:21791:23272/2
-CATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTAT
-+
-BBBBBFFFFFFFGGGGGGGGGGHHHHGGHHHHHHHGGGEGGHHFGDHGHHHHHHGGHHH2GGHHHGHHGGGGFGFBFHHHGGGGHGHGHHHHHHHHHHGHGHHGHHGGHGGHHHHGGFHGGGHFHGHHHHHGGHHHGHGFCGFFDGGG/FCFHBGHHHHHHHGHCHHHHGGGHGHGHGCFF;>@DFFBFFFFFFDFFFFFFFF;;.DDAA;FFF;BB;C@..9::BBFBBA;-:.::
-@M01368:8:000000000-A3GHV:1:1108:23314:26089/2
-CATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATT
-+
-BBAAAFFFFFFFGGGGGGGGGGHHGDHHHHHFHHHHGGGGGFHFFGFFHEGHHHHHHHHFGHFHHHHDEHHHHHHGHFHHGGGGHHGFHHHHHHHHHHHHHHHHHHF?EEFCHFGFHHEFFFHGFHHGHFHFHHHFFHAG??GHACGGDGHGGBFHHHHHHHGHFGHGHGEGHGCFFF@CD-BDDFFFFFFFFDDFFBBFFD-B-A.DFFA.AFEFF-;-..:..BFFFFFA-../9FFF//B/BBBBF
-@M01368:8:000000000-A3GHV:1:1108:25084:18127/2
-CCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAG
-+
-CCCCCFFFFFFFGGGGGGGGGGGGHHHHHHHHFHHHHHHHHHHHHHHGGHHGHHHHHGGGGFHHHHHFHHHHHHHHHGGHHHHHCGGHGHHGGGGGGGHHGGGGGGGGHHHGHHGGGGGGGGGGGGGGGG@CDE?EFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFFFE;DFBFFFFFFFFFFFFFFFFFFFAFFFFFFFBFFE0BBBFFFFFFFFFFBFFFF9BB0:0:B;
-@M01368:8:000000000-A3GHV:1:1108:7626:10048/2
-AACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGAT
-+
-BCCDDFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHHHHHHHGGGGGHHHHHHHHFGHHHHHHHHHHHHGHHHHGGFHHHFGGGGHHHGHHHHHHHHHHHFGHHHHHGHGHGHHGCGCGGFHHHGGGGGGGGGGGGGGGGGGGFFFAFFFFFFAA-AFEFFFFFFFFFFFFFFFFFFFFFFFFF
-@M01368:8:000000000-A3GHV:1:1109:11270:12255/2
-CTACCTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATT
-+
-BCCCCFFFFFFFGGGGGGGGGGHHHHHFHHHHGGGGHHHHHHHHHHHHHHHHHHHHGHGGHGGHHGHHHHHGHHHHHHHHHFHHHHHGHHGHHHHHGGGGGHHHHHHHHGHHHHHH?FHHHHHGHGHGHHGGGGGGGGGGGGGGGGGGGGFFFFFFFFFFFFFFFFECADFFFF.DEBFFFFFF;D.B;.ED.FFFFFFF-.:00
-@M01368:8:000000000-A3GHV:1:1109:24217:19077/2
-TAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGAACCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTT
-+
-BBBBBFFFFFFFGFEGGCFGFGHHHHFHHFHHHHHHGGEEEFGHGFFAGFFEEAEGGHHBEEFAFGAEF1G3GFGGEEF@EAGGHGFF?ECGGHEEEFGGHHHHFHHFHHGFHHHC2DAGHGG00-BFFA9FFFFFFFFFFFBF
-@M01368:8:000000000-A3GHV:1:1109:27103:7153/2
-NTACTTCAGGGTCNTAAAACCTNAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCANGGNAGCTCTCCNNGNNNNTGGTATTNTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTAC
-+
-#>>AAAFFFFFFG#AAFFGGGG#BAFFAGHHHGGGGHGHHHHFHHHHHHHGHHHHHHHEGFGGHHBGHGHHBGFFEG2GHHFHFHHHGHHGHFH#/B#??FFGHHH##?####????FHG#??CFGGGEF>CGAGAGGGFGAA@@FFFFFFFFB=@@FF?FF;FFFF@@-@-AFEF?BB/BF?E-BBFFF;:BB/BFFFB/BFFFBFBFBBFFFFFB/FFFBB/;9A@@BFFBFFFF/BBFFFF
-@M01368:8:000000000-A3GHV:1:1109:8763:7859/2
-GGTTCCTACTTCAGGGTCATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAG
-+
->AAABFFFFFFFGGGGGGGGGGHHHHHHHCHFAFHHDGEGFHHHHHHHHFHHHHFHHHHHHGHGGHGGHHHFHGHHHHHFFHHHHHHBGHFFHGHGHHHHHGGEGGHHHHHGHHHHHHHHHGFGHHHHGHGHGHHCCGGGGGGGGGGAAGF?FFFFBFA@=BDDFF?-;FFF;@CFFEFEFFFFFFFFFBFDFFFFFFFFFFFFFFFFFFFFFF:FFFF/BF/FFFEFF?DECAFEFFFFFE:FFFFFFFB
-@M01368:8:000000000-A3GHV:1:1110:12808:18367/2
-GCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACAGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATT
-+
-ABCCCCDDDFFFGGGGGGGGGGHHHHHHHHHGGGGGGHHHHFHHGHHHHHHHHHHHHHGHHHGGHHHH3GFHEFHHHHHGHHGFHHHHHFGHHHHHGHGHGGHGGGGGGGHHHGGCGGGGHHGGGGGGGGGGGGGGGGGGGF-BAEFEBEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFFAFEFDFFFFFFFFFF0BFBED=@DFFFFFFFFFEFFFFFBFAFB;0:
-@M01368:8:000000000-A3GHV:1:1110:14751:26540/2
-ATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGGTTGGGCTATTTGGGTTTTTTGACC
-+
-BBBBBFBBBBBFGGGGGGGGGGHHHHGGFHGGHGGCHHHHHHGHHHGHHGEGGGHHHHHHHHHHEFHHHHHHGEE?FGHGEHGGHHHGGGGGGEHHGGGGHHGGGEGHHHGHGGGDFGHHGHGGGGGCGAFFCCDFHHHH1CGGHBHFHFCGFFCDFHGCCE.CC:EGFHHCGHHGCF?FGGGFGGG;B9;FFFFFFEGEBF0;;;0;CFBFFF?D.DE.:..99-...;;//9A.;DE.9-./:
-@M01368:8:000000000-A3GHV:1:1110:15519:10008/2
-GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACA
-+
-BBCBCFFFFFFFGGGGGGGGGGHHHGHHHFHHBGHHHGGGGHHHHHHGHHHHHHHHHHHHHHHGGHGGHHGHHHGGBGHHHHHHHHHHHHHHHHHGGFHHHGGGEGHHHHHHHHHHHHHHHGHHHHHHGHGHGHHGGGGGGDGGGGGGGGGBFFFFFFFFFFFFFFFFFFFECB-=BFFFFFFFFBB?E-A::FFBFFFFFBFFFFFFFFFFFFBFFEFFF;0BFF0:FE?FFFFBFFFFFFFFFFFFFF
-@M01368:8:000000000-A3GHV:1:1110:16559:7507/2
-GCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAAT
-+
->AABBCCCCFFFGGGGGGGGGGHHHHHHHHHGGHGGHGHFHHHHFHHHHHHHHHHFHHGHHHHGHHHHHGGEEGHHHHHHHHHHHHHHHHHHHHHHGHGHGHHGGGGGGGHHGGGFGAGFGGGGGGGGGGGDDDFAGGFFFFCFAFEEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFFFFFFFFFFFFFFE..@BFFBD./;:0FFFFF09B/AFCA=-99BFFBFFFFFFFB:BFBFFBFB9:
-@M01368:8:000000000-A3GHV:1:1110:16700:6843/2
-GTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTT
-+
-CBCCDFFFFFFFGGGGGGGGGGHHGHHHGGHFHHHGGGGGHHHHHHHHHHGHHHHHHHHHHHGGHGGHHHHHHHGBGFFHHHHHHGHHHHHHHHGHHHHHGGGGGHHHHHHHHHHHHHHHFHHHHGHCG/GGHHGGGGGGAFGEEADAGGDFFFF00D>CFDDFFFB?AF?B;-@>.FEDFFFFF0BF-BDFFFFE00B0BF0FFFF:BFA0B0:BBFBBFFFFF
-@M01368:8:000000000-A3GHV:1:1110:18014:7856/2
-CATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTAT
-+
-CDDDBFCDDDDFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHGGGGGHHHHHHHHHGHHHHHEGHHHHHEHGHGHHGGGCCGGHGHGCGGGGDHHHHFHGGGGGGGGGGGFGGFGAC-EFEGGGFFFGFF;DFFFBFFFFFFFFFFFFFFFFFFFEFFFBFFFFFFFFF
-@M01368:8:000000000-A3GHV:1:1110:23303:11846/2
-GTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTAAGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTA
-+
-BBCBBFFFFFCFGGGGGGGGGGHHHHHHGGHHHHHHHHGH5GHHHGGGGHHHHHFHHHHHHHEHHGFBGEEFE1FG5GFHHHHHFHHHGGGGGHHHHHHHHHHHGGHHHHHGGGGFHHFHH/EHHHGG?@EFHHHGGGGHFGGGGHHFHGGDGGGGFFFHGFDGGGCGACC;DFFB0CH0CCCFGBFBFB/:A?FFFE?AA.CFBECE0BBBF0F/A./9FFFFF./;/FFFFFFA.:////9BB/B/BB
-@M01368:8:000000000-A3GHV:1:1111:13195:14562/2
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGAT
-+
-ABBBBFBBBBBFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHDGHHHGGGGHHHHHHHHHHHHGHHHHHHHHHGGGGGHHGHHHHGFGFHHHHHHHHGHHHHHHHGHHHHHGGGGGHHHHGHHHHHHHHHHGFHHHHHGFGHGHHGGGGFCGFGFFFFAFFFFFFFFFFA;BADFFFAFFFFFFFFFEFFFEFFFFFFF;FDBFFFFFFFFFFFF
-@M01368:8:000000000-A3GHV:1:1111:14287:20817/2
-ATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATGAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGTTAGACCTGTGATC
-+
-CCCCBFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHHHHHHHHHGHHHGHHHHGHHHGHGHFHHHGGGGHHHHHHHHBGGGFHHFGGHHGGHGHHHHHHHHEGFFHHFGGGGGHHEHHHBHHHHHFFHHGHGGGGGHFGF3FDGHHGCGGFGHHGGGG/2GG@E?GHHHGFEDGGCFDFFFFFFFFFFFBFFFFFFFFFFF/FFF/BFFFFFFFBA.?@;FFBDFFFF
-@M01368:8:000000000-A3GHV:1:1111:7437:24508/2
-ACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGT
-+
-BBBBBFFBABBBGGGGGGGGFGHHHHHHHHHHHHHGHHHGFHHHHGGGGDHHHHHHHHGFHHHHHGHHHHHHGHFHGHHGGGDFGDFFGGGFFGFFGHGHHHGGC?AD-:A-AFFGGG-?9EFEFGGFFBFEFCFFFFFFFBBBFFFFFFFFFFFFFFFBBFFFFFFFFFFFFFFFFFFEFFFFFFBFFFFEFEFFFF;CF-FFFFFFFF/BFFFFFFF
-@M01368:8:000000000-A3GHV:1:1111:8665:25542/2
-CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGACAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTT
-+
-CCCCCFFFFFFFGGGGGGGGGGHHHHHHHFHHHHCHHGDGGGGHGGHHHHHHHHGHGHHHGGGGGHFHHHHFHHHFFHHHHHFGCGGEHFHHFGGHFHGGGGGGHHFEEEAFEFFDFHGHHHHGGGGGGGHH0DGGGGCGHCC-FFFFFFFGGGGGGGGGGHHHHHGHHHHGHHGGHHGGHGGHHHGHHHHFHHGHHAFGGGHHHHHGHHHHFHFHHHHHECGGGHGHHHEFHFHGGGGGGGGFGFFGFFEFGEGHHHGHHGGDGGGHGFGGGGGGGGE@GCGGHHHGHHHHHGHHHHFHHGEGFFEGCE.ECCECEHCGGFFE/AABFFG0CFE0BFFF9CFGG.F/BBBFFGFGGGFFFFFFBFEDAEFFFFFBFFFFFFF0;FEBF:
-@M01368:8:000000000-A3GHV:1:1112:10272:7828/2
-GTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATAC
-+
-BBCCBFFCCCCCGGGGGGGGGGGGGGHHHHHHGGGGGGHHFHGGGGGFGHGGGGGGHHHHHHHHHHFHHHHFHGFFHHGF?ECEFFFFFFHHHHHH?FHHHHHHHHHGFHHHHHHGGGFGHHHHFHHHHHHHHHHGGGFGFGCGGGGHHHHHHHHHHHGGFFHGHHHHHFFHFCGFHCCGHGHCGGHG?DGG
-@M01368:8:000000000-A3GHV:1:1112:9832:16531/2
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTA
-+
-BBBBBFBBBBBFGGGGGGGGGGHFHHHHHHHHHHHHHHGHHHHGHFHHHGEEGGHHHGHHHHHHHHHHHHEHGHHGGGHGGHGHHHHHGHHHHGHHHHHHHHHHHHHHGHHHHHGEGCGHHHHHGHHHHHHHHHHHHHHGHFFGHGHHGGGDACDFFFFABFFFFFFFFFFD-BC?DA9.DFFBEA==B=?F.;ABBFBFEF-DAF/BFF9/FFFBB/9BFFFFFFF/BFBBFFFFFFF/9FEA?--B/
-@M01368:8:000000000-A3GHV:1:1112:9832:6701/2
-GCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATT
-+
-BBBBAFFFFFFFGGGGGGGGGGFGHHHHHGHHHHHHHHHHHHCGHHHHHHHGHHHHHHDGHHGGAECHHGHHHHHHHGHHHGHGFHHHHGGHGGHHFHHHHHHFHHHHHHHHH2GHHHHGHGHHFHHGGFGGGHHGHHHHHFHHHHEFHHHHEFGFFHGEHCFGDFDFFFFFCFFFFFFFFFFFFFAFBFFDFFFFFFFFADCF?9?EFBFB9EF-A.;BFFFFFFFBFFFFFFFFFFFFBFFFFFFFFFF
-@M01368:8:000000000-A3GHV:1:1113:13532:8618/2
-GCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTGGAT
-+
-CDDDBFFFFFFFGGGGGGGGGGHHHHHGGHHHGHHHGHHGGGGEHHGGHHHHHHHHHHHHHHGHHGHHHHHGGHHHHHFHHHHGCDCGHHHHHHHHEHHHGHGHHHGFHGGHGGHHHHHHHHFHHHFHHHGHHHHHHHHHFGGHHHHGGGEGAFHHGHHGGHHHHGHFBGFHGHGHGHGGHGGF@DAFEFBECAB=A.0BFFFEDF=A9.@D.-AAFFFA-:-..:.9/0:;0..-..0000::BFB09::
-@M01368:8:000000000-A3GHV:1:1113:21679:18011/2
-TTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATC
-+
-BCCCBFFFFFFFGGGGGGGGGGHHHHHFHHHGHHHGFGHGGHGGHHHHHHGHHHHHHHGGGGFHHHHHHEGHHHHHHHHFGGFCGEHFHFGEGHHHFGGGGGHHHGGGEHGGGGGHHHHHHGDCCCGHHHHDGGGGCD/FCC->DFF
-@M01368:8:000000000-A3GHV:1:1113:5741:16959/2
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTA
-+
-BBBBCFCCCCCFGGGGGGGGGGHHHHHHHGHHHHHHHHGHHHHGHDGHHHGGGGHHHHHHHHHHHHHHHGHHHHHGGGEGGHHHHFHHHHHHHHHHHHHHHHHHHHHHGHHGHHGGGGGHHHHHHHHHHHGHHHFHHHHHHGHGHGHGGGGCGGFGGFFFFFFFFFFFFFBFFFFFFFFFFFFFFAFFFFEAEFFFFFFFFFFF9BFFBFFFFFFFFFFFFFFBFFFFFFFFFFFFFFFFFFADAB-/B
-@M01368:8:000000000-A3GHV:1:1114:10130:11959/2
-ATCAGAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACG
-+
-BCCDDFFFFFFFGGGGGGGGGGGHHHHHGGHHHGGGCGGHGHGGGGHHGGGGHHHHHHGGGEGGHHHFGGGGG?E1FE?/EEHHHHHGHHGHHHHGHFHGHGHHGDGGFG2FF2?GHHHHHGCCCFHGHGHHHHGHHFEHHFGHHGHH<1=DGHHHGHHGHGAGAE
-@M01368:8:000000000-A3GHV:1:1114:14540:5315/2
-CACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGC
-+
-CCCCCFFCCDCCGGGGGGGGGGHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHHGHHHHHHHGHHHHFHFHHHGGGGGHHHHHHHHHHHHHGHHHHHHHHGGGGGGHHHFHHHGHHHHHHHHHHHHHHHHHHGHGHHHGGGFGHHHHHHHHFHHHHHF?1FHHGHGHGHGHHGGFFFFDBFBE;BCC.:BFFFFFFFFFFFFFF;AFFFFF-=-.AEDEFFFFF..9A;9FFFF0FFFFE00FFF0:B
-@M01368:8:000000000-A3GHV:1:1114:15066:16302/2
-TTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAG
-+
-CCCCCFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHHHDGHHHHFHHGHHGHHHHHGGGGEHHHHFHHFF5FHHFEGHHHGDHGGHGHGFGGGEHFGHHGGGGGHGHHHGHHFHHB3FGHHFGGGG?GFFHCCEBGFFECCDFEGFCFGCHHGFDDHHHGHHCFGGGGGFBFDGFG?-:..AFG.-C0C009;:00;00:9/:CEFFF?AE::9;9?0:FEF0;
-@M01368:8:000000000-A3GHV:1:1114:16639:15258/2
-TTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCGCACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGAT
-+
-CDCCCFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHGHHHHHHGGGGGHGIHHHIH5DEGHHHF?FGHGGHGGHEGGHFHHGHGEHHGGGGGFFFGHFBG2GHEBGHHGHGGEG/GFGABEDFGHEED?GGHHFFGGGCFEGD/GFHFFGEFGCGG?CC??D-EF@EEEFGCDDBBFGGGEBBFFF09090A.BFGA.9CCA0;EBAB00BBFF.@-./;BB;BFFF0:00099AAFFF
-@M01368:8:000000000-A3GHV:1:1114:2404:13066/2
-ATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGACCATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGA
-+
-CCCCCFFFFFFCGGGGGGGGGGHHHHHHHHHFFHHHHGGGGGHFFFHHFHHHHHHHHHHHHHHHGFEGGGHGEDFCDFHGHFG@@DGGHHHHHHGGGGCGGGGGEHGGCGBB?CF99EGFGGFGG?D9CFFFF/BBFFFFFEF9BFFAFFFFEFFFFFFFFFFFFFFFFFFFFF.FFBBFFFFFFFFFFFF-9;;;BFFFFFB9BFBFBFABFFEFFFFFFFFFF::BFFBFF
diff --git a/tools/trim_galore/test-data/paired_example_results2.txt b/tools/trim_galore/test-data/paired_example_results2.txt
index b088f08509..96f1f64acc 100644
--- a/tools/trim_galore/test-data/paired_example_results2.txt
+++ b/tools/trim_galore/test-data/paired_example_results2.txt
@@ -1,50 +1,38 @@
-
SUMMARISING RUN PARAMETERS
-==========================
+=========================
Input filename: input_1.fastq
Trimming mode: paired-end
-Trim Galore version: 0.6.10
-Cutadapt version: 5.0
-Python version: 3.12.10
-Number of cores used for trimming: 4
+Trim Galore version: 2.2.0 (Oxidized Edition)
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
-Using Nextera adapter for trimming (count: 29). Second best hit was smallRNA (count: 0)
-Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected)
-Maximum trimming error rate: 0.1 (default)
+Adapter sequence: 'CTGTCTCTTATA' (user-specified or auto-detected)
+Maximum trimming error rate: 0.1
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp
-This is cutadapt 5.0 with Python 3.12.10
-Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq
-Processing single-end reads on 4 cores ...
+Trim Galore 2.2.0 (Oxidized Edition) — adapter trimming built in
+This is cutadapt 4.0 (compatible; for MultiQC backwards compatibility)
+Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq
+Processing reads on 1 core in single-end mode ...
=== Summary ===
-Total reads processed: 99
-Reads with adapters: 52 (52.5%)
-Reads written (passing filters): 99 (100.0%)
+Total reads processed: 99
+Reads with adapters: 52 (52.5%)
+Reads written (passing filters): 99 (100.0%)
-Total basepairs processed: 24,849 bp
-Quality-trimmed: 205 bp (0.8%)
-Total written (filtered): 23,339 bp (93.9%)
+Total basepairs processed: 24,849 bp
+Quality-trimmed: 205 bp (0.8%)
+Total written (filtered): 23,339 bp (93.9%)
=== Adapter 1 ===
-Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times
+Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times.
-Minimum overlap: 1
No. of allowed errors:
1-9 bp: 0; 10-12 bp: 1
-Bases preceding removed adapters:
- A: 9.6%
- C: 38.5%
- G: 23.1%
- T: 28.8%
- none/other: 0.0%
-
Overview of removed sequences
length count expect max.err error counts
1 11 24.8 0 11
@@ -55,78 +43,70 @@ length count expect max.err error counts
13 2 0.0 1 2
14 1 0.0 1 1
16 1 0.0 1 1
-17 1 0.0 1 0 1
-20 2 0.0 1 2
-21 1 0.0 1 1
-24 1 0.0 1 1
-26 2 0.0 1 2
-31 1 0.0 1 1
-33 1 0.0 1 1
-41 2 0.0 1 2
-49 1 0.0 1 1
-50 1 0.0 1 1
-54 1 0.0 1 1
-56 1 0.0 1 1
-58 2 0.0 1 2
-60 1 0.0 1 1
-67 2 0.0 1 2
-68 1 0.0 1 1
-69 1 0.0 1 1
-73 1 0.0 1 1
-80 1 0.0 1 1
-86 1 0.0 1 1
+17 1 0.0 1 1
+20 2 0.0 2 2
+21 1 0.0 2 1
+24 1 0.0 2 1
+26 2 0.0 2 2
+31 1 0.0 3 1
+33 1 0.0 3 1
+41 2 0.0 4 2
+49 1 0.0 4 1
+50 1 0.0 5 1
+54 1 0.0 5 1
+56 1 0.0 5 1
+58 2 0.0 5 2
+60 1 0.0 6 1
+67 2 0.0 6 2
+68 1 0.0 6 1
+69 1 0.0 6 1
+73 1 0.0 7 1
+80 1 0.0 8 1
+86 1 0.0 8 1
+
RUN STATISTICS FOR INPUT FILE: input_1.fastq
=============================================
99 sequences processed in total
-
+Sequences removed because they became shorter than the length cutoff of 20 bp: 1 (1.0%)
+Sequences removed because they were longer than the upper length cutoff: 0 (0.0%)
+Sequences removed because of too many N bases: 0 (0.0%)
SUMMARISING RUN PARAMETERS
-==========================
+=========================
Input filename: input_2.fastq
Trimming mode: paired-end
-Trim Galore version: 0.6.10
-Cutadapt version: 5.0
-Python version: 3.12.10
-Number of cores used for trimming: 4
+Trim Galore version: 2.2.0 (Oxidized Edition)
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
-Using Nextera adapter for trimming (count: 29). Second best hit was smallRNA (count: 0)
-Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected)
-Maximum trimming error rate: 0.1 (default)
+Adapter sequence: 'CTGTCTCTTATA' (user-specified or auto-detected)
+Maximum trimming error rate: 0.1
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp
-This is cutadapt 5.0 with Python 3.12.10
-Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq
-Processing single-end reads on 4 cores ...
+Trim Galore 2.2.0 (Oxidized Edition) — adapter trimming built in
+This is cutadapt 4.0 (compatible; for MultiQC backwards compatibility)
+Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq
+Processing reads on 1 core in single-end mode ...
=== Summary ===
-Total reads processed: 99
-Reads with adapters: 58 (58.6%)
-Reads written (passing filters): 99 (100.0%)
+Total reads processed: 99
+Reads with adapters: 58 (58.6%)
+Reads written (passing filters): 99 (100.0%)
-Total basepairs processed: 24,849 bp
-Quality-trimmed: 745 bp (3.0%)
-Total written (filtered): 23,035 bp (92.7%)
+Total basepairs processed: 24,849 bp
+Quality-trimmed: 745 bp (3.0%)
+Total written (filtered): 23,035 bp (92.7%)
=== Adapter 1 ===
-Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times
+Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times.
-Minimum overlap: 1
No. of allowed errors:
1-9 bp: 0; 10-12 bp: 1
-Bases preceding removed adapters:
- A: 12.1%
- C: 37.9%
- G: 8.6%
- T: 41.4%
- none/other: 0.0%
-
Overview of removed sequences
length count expect max.err error counts
1 16 24.8 0 16
@@ -142,29 +122,39 @@ length count expect max.err error counts
16 1 0.0 1 1
17 1 0.0 1 1
19 2 0.0 1 2
-21 1 0.0 1 1
-25 1 0.0 1 1
-30 1 0.0 1 1
-32 2 0.0 1 2
-34 1 0.0 1 1
-36 2 0.0 1 2
-38 1 0.0 1 1
-40 1 0.0 1 1
-41 1 0.0 1 1
-42 1 0.0 1 1
-43 1 0.0 1 1
-49 1 0.0 1 1
-51 1 0.0 1 1
-56 1 0.0 1 1
-57 1 0.0 1 1
-60 1 0.0 1 1
-67 1 0.0 1 1
-80 1 0.0 1 1
+21 1 0.0 2 1
+25 1 0.0 2 1
+30 1 0.0 3 1
+32 2 0.0 3 2
+34 1 0.0 3 1
+36 2 0.0 3 2
+38 1 0.0 3 1
+40 1 0.0 4 1
+41 1 0.0 4 1
+42 1 0.0 4 1
+43 1 0.0 4 1
+49 1 0.0 4 1
+51 1 0.0 5 1
+56 1 0.0 5 1
+57 1 0.0 5 1
+60 1 0.0 6 1
+67 1 0.0 6 1
+80 1 0.0 8 1
+
RUN STATISTICS FOR INPUT FILE: input_2.fastq
=============================================
99 sequences processed in total
+Sequences removed because they became shorter than the length cutoff of 20 bp: 1 (1.0%)
+Sequences removed because they were longer than the upper length cutoff: 0 (0.0%)
+Sequences removed because of too many N bases: 0 (0.0%)
+
+=== Paired-end validation ===
+
+Number of sequence pairs analysed: 99
-Total number of sequences analysed for the sequence pair length validation: 99
+Number of sequence pairs removed because at least one read was shorter than the length cutoff (1.01%):
+ 1
+Number of pairs removed for N content (0.00%):
+ 0
-Number of sequence pairs removed because at least one read was shorter than the length cutoff (20 bp): 1 (1.01%)
diff --git a/tools/trim_galore/test-data/paired_example_results2gz.txt b/tools/trim_galore/test-data/paired_example_results2gz.txt
index 29e14a56c6..ba07518a69 100644
--- a/tools/trim_galore/test-data/paired_example_results2gz.txt
+++ b/tools/trim_galore/test-data/paired_example_results2gz.txt
@@ -1,51 +1,39 @@
-
SUMMARISING RUN PARAMETERS
-==========================
+=========================
Input filename: input_1.fastq.gz
Trimming mode: paired-end
-Trim Galore version: 0.6.10
-Cutadapt version: 5.0
-Python version: 3.12.10
-Number of cores used for trimming: 4
+Trim Galore version: 2.2.0 (Oxidized Edition)
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
-Using Nextera adapter for trimming (count: 29). Second best hit was smallRNA (count: 0)
-Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected)
-Maximum trimming error rate: 0.1 (default)
+Adapter sequence: 'CTGTCTCTTATA' (user-specified or auto-detected)
+Maximum trimming error rate: 0.1
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp
Output file will be GZIP compressed
-This is cutadapt 5.0 with Python 3.12.10
-Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq.gz
-Processing single-end reads on 4 cores ...
+Trim Galore 2.2.0 (Oxidized Edition) — adapter trimming built in
+This is cutadapt 4.0 (compatible; for MultiQC backwards compatibility)
+Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq.gz
+Processing reads on 1 core in single-end mode ...
=== Summary ===
-Total reads processed: 99
-Reads with adapters: 52 (52.5%)
-Reads written (passing filters): 99 (100.0%)
+Total reads processed: 99
+Reads with adapters: 52 (52.5%)
+Reads written (passing filters): 99 (100.0%)
-Total basepairs processed: 24,849 bp
-Quality-trimmed: 205 bp (0.8%)
-Total written (filtered): 23,339 bp (93.9%)
+Total basepairs processed: 24,849 bp
+Quality-trimmed: 205 bp (0.8%)
+Total written (filtered): 23,339 bp (93.9%)
=== Adapter 1 ===
-Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times
+Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times.
-Minimum overlap: 1
No. of allowed errors:
1-9 bp: 0; 10-12 bp: 1
-Bases preceding removed adapters:
- A: 9.6%
- C: 38.5%
- G: 23.1%
- T: 28.8%
- none/other: 0.0%
-
Overview of removed sequences
length count expect max.err error counts
1 11 24.8 0 11
@@ -56,79 +44,71 @@ length count expect max.err error counts
13 2 0.0 1 2
14 1 0.0 1 1
16 1 0.0 1 1
-17 1 0.0 1 0 1
-20 2 0.0 1 2
-21 1 0.0 1 1
-24 1 0.0 1 1
-26 2 0.0 1 2
-31 1 0.0 1 1
-33 1 0.0 1 1
-41 2 0.0 1 2
-49 1 0.0 1 1
-50 1 0.0 1 1
-54 1 0.0 1 1
-56 1 0.0 1 1
-58 2 0.0 1 2
-60 1 0.0 1 1
-67 2 0.0 1 2
-68 1 0.0 1 1
-69 1 0.0 1 1
-73 1 0.0 1 1
-80 1 0.0 1 1
-86 1 0.0 1 1
+17 1 0.0 1 1
+20 2 0.0 2 2
+21 1 0.0 2 1
+24 1 0.0 2 1
+26 2 0.0 2 2
+31 1 0.0 3 1
+33 1 0.0 3 1
+41 2 0.0 4 2
+49 1 0.0 4 1
+50 1 0.0 5 1
+54 1 0.0 5 1
+56 1 0.0 5 1
+58 2 0.0 5 2
+60 1 0.0 6 1
+67 2 0.0 6 2
+68 1 0.0 6 1
+69 1 0.0 6 1
+73 1 0.0 7 1
+80 1 0.0 8 1
+86 1 0.0 8 1
+
RUN STATISTICS FOR INPUT FILE: input_1.fastq.gz
=============================================
99 sequences processed in total
-
+Sequences removed because they became shorter than the length cutoff of 20 bp: 1 (1.0%)
+Sequences removed because they were longer than the upper length cutoff: 0 (0.0%)
+Sequences removed because of too many N bases: 0 (0.0%)
SUMMARISING RUN PARAMETERS
-==========================
+=========================
Input filename: input_2.fastq.gz
Trimming mode: paired-end
-Trim Galore version: 0.6.10
-Cutadapt version: 5.0
-Python version: 3.12.10
-Number of cores used for trimming: 4
+Trim Galore version: 2.2.0 (Oxidized Edition)
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
-Using Nextera adapter for trimming (count: 29). Second best hit was smallRNA (count: 0)
-Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected)
-Maximum trimming error rate: 0.1 (default)
+Adapter sequence: 'CTGTCTCTTATA' (user-specified or auto-detected)
+Maximum trimming error rate: 0.1
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp
Output file will be GZIP compressed
-This is cutadapt 5.0 with Python 3.12.10
-Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq.gz
-Processing single-end reads on 4 cores ...
+Trim Galore 2.2.0 (Oxidized Edition) — adapter trimming built in
+This is cutadapt 4.0 (compatible; for MultiQC backwards compatibility)
+Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq.gz
+Processing reads on 1 core in single-end mode ...
=== Summary ===
-Total reads processed: 99
-Reads with adapters: 58 (58.6%)
-Reads written (passing filters): 99 (100.0%)
+Total reads processed: 99
+Reads with adapters: 58 (58.6%)
+Reads written (passing filters): 99 (100.0%)
-Total basepairs processed: 24,849 bp
-Quality-trimmed: 745 bp (3.0%)
-Total written (filtered): 23,035 bp (92.7%)
+Total basepairs processed: 24,849 bp
+Quality-trimmed: 745 bp (3.0%)
+Total written (filtered): 23,035 bp (92.7%)
=== Adapter 1 ===
-Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times
+Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times.
-Minimum overlap: 1
No. of allowed errors:
1-9 bp: 0; 10-12 bp: 1
-Bases preceding removed adapters:
- A: 12.1%
- C: 37.9%
- G: 8.6%
- T: 41.4%
- none/other: 0.0%
-
Overview of removed sequences
length count expect max.err error counts
1 16 24.8 0 16
@@ -144,29 +124,39 @@ length count expect max.err error counts
16 1 0.0 1 1
17 1 0.0 1 1
19 2 0.0 1 2
-21 1 0.0 1 1
-25 1 0.0 1 1
-30 1 0.0 1 1
-32 2 0.0 1 2
-34 1 0.0 1 1
-36 2 0.0 1 2
-38 1 0.0 1 1
-40 1 0.0 1 1
-41 1 0.0 1 1
-42 1 0.0 1 1
-43 1 0.0 1 1
-49 1 0.0 1 1
-51 1 0.0 1 1
-56 1 0.0 1 1
-57 1 0.0 1 1
-60 1 0.0 1 1
-67 1 0.0 1 1
-80 1 0.0 1 1
+21 1 0.0 2 1
+25 1 0.0 2 1
+30 1 0.0 3 1
+32 2 0.0 3 2
+34 1 0.0 3 1
+36 2 0.0 3 2
+38 1 0.0 3 1
+40 1 0.0 4 1
+41 1 0.0 4 1
+42 1 0.0 4 1
+43 1 0.0 4 1
+49 1 0.0 4 1
+51 1 0.0 5 1
+56 1 0.0 5 1
+57 1 0.0 5 1
+60 1 0.0 6 1
+67 1 0.0 6 1
+80 1 0.0 8 1
+
RUN STATISTICS FOR INPUT FILE: input_2.fastq.gz
=============================================
99 sequences processed in total
+Sequences removed because they became shorter than the length cutoff of 20 bp: 1 (1.0%)
+Sequences removed because they were longer than the upper length cutoff: 0 (0.0%)
+Sequences removed because of too many N bases: 0 (0.0%)
+
+=== Paired-end validation ===
+
+Number of sequence pairs analysed: 99
-Total number of sequences analysed for the sequence pair length validation: 99
+Number of sequence pairs removed because at least one read was shorter than the length cutoff (1.01%):
+ 1
+Number of pairs removed for N content (0.00%):
+ 0
-Number of sequence pairs removed because at least one read was shorter than the length cutoff (20 bp): 1 (1.01%)
diff --git a/tools/trim_galore/test-data/paired_hardtrimmed3_pair1_.fastq.gz b/tools/trim_galore/test-data/paired_hardtrimmed3_pair1_.fastq.gz
new file mode 100644
index 0000000000..01f07eb87e
Binary files /dev/null and b/tools/trim_galore/test-data/paired_hardtrimmed3_pair1_.fastq.gz differ
diff --git a/tools/trim_galore/test-data/paired_hardtrimmed3_pair1_.fastqsanger b/tools/trim_galore/test-data/paired_hardtrimmed3_pair1_.fastqsanger
deleted file mode 100644
index c0ee2935c2..0000000000
--- a/tools/trim_galore/test-data/paired_hardtrimmed3_pair1_.fastqsanger
+++ /dev/null
@@ -1,396 +0,0 @@
-@M01368:8:000000000-A3GHV:1:1101:6911:8255/1
-TCGCACCTACGTTCAATATT
-+
-FFEFE;DFFFDFBFF/9BFB
-@M01368:8:000000000-A3GHV:1:1101:14518:9998/1
-GACCTGTGATCCATCGTGAT
-+
-FF//;BFFFFFBF/9D:A//
-@M01368:8:000000000-A3GHV:1:1101:18422:19051/1
-TATTATTTATCGCACCTACG
-+
-FFEFBFFFFFFFFFF:FFF.
-@M01368:8:000000000-A3GHV:1:1101:25545:21098/1
-TTTAAGGGGAACGGGTGGGG
-+
-FBFFFFFFBF..A..;@B--
-@M01368:8:000000000-A3GHV:1:1101:5446:12248/1
-AAGGGGAACGTGTGGGCTAT
-+
-FFFBFFFFFFEFFFFFEFFF
-@M01368:8:000000000-A3GHV:1:1101:5861:6452/1
-TGATCCATCGTGATGTCTTT
-+
-FFFFFBFFBD.AFFFFFFF0
-@M01368:8:000000000-A3GHV:1:1102:10403:6021/1
-ACCCTATGTCGCAGTATCTG
-+
-..9..9/9;BEF;A:9/BFF
-@M01368:8:000000000-A3GHV:1:1102:10677:23253/1
-AATTCTGTCTCTTATACACA
-+
-F0FFFFFFFFFFFFFFFFF:
-@M01368:8:000000000-A3GHV:1:1102:13809:1733/1
-GTCTCTTATACACATCTCCG
-+
-FBBBFFFFFBFF0BFBF9F.
-@M01368:8:000000000-A3GHV:1:1102:17584:10050/1
-AATCTCGTATGCCGTCTTCT
-+
-FF;.BF9ADF/9;A;DFFF0
-@M01368:8:000000000-A3GHV:1:1102:18842:24844/1
-TCTGTCTTTGATTCCTGCCT
-+
-BBBFBFBFFF//BFB/BFF/
-@M01368:8:000000000-A3GHV:1:1102:21788:11027/1
-ACTAAAGTGTGTTAATTCTG
-+
-FBFF0FFBBBBFBFBFFFF0
-@M01368:8:000000000-A3GHV:1:1102:5728:8777/1
-CCTGAAGTAGGAACCAGATG
-+
-FFFFBBFFFFF/FFEFFFBB
-@M01368:8:000000000-A3GHV:1:1102:5983:21093/1
-TATTATTTATCGCACCTACG
-+
-F0BFFFBBFF0EFFFFFFFA
-@M01368:8:000000000-A3GHV:1:1102:7765:18353/1
-CCTATGTCGCAGTATCTGTC
-+
-.::FBFBFFE-A9:BFFFF0
-@M01368:8:000000000-A3GHV:1:1103:10405:17879/1
-TATTATTTATCGCACCTACG
-+
-FBFFFFFFFFFFFFFFFFF?
-@M01368:8:000000000-A3GHV:1:1103:13493:8205/1
-GATTCCTGCCTCATCCTATT
-+
-FFFFFFFFBFBBBFFFFEFF
-@M01368:8:000000000-A3GHV:1:1103:14060:21273/1
-TATTACTGTCTCTTATACAC
-+
-:FFFBB/BFFFFFBFFFFB/
-@M01368:8:000000000-A3GHV:1:1103:14528:12993/1
-TCGCAGTATCTGTCTTTGAT
-+
-BFEA-A.B//9F9F//;BBB
-@M01368:8:000000000-A3GHV:1:1103:17657:20856/1
-TGCCTCATCCTATTATTTAT
-+
-FFFFFFFFFFF/FFFFBFF9
-@M01368:8:000000000-A3GHV:1:1103:23099:3794/1
-GCGAATCTCGGATGCCGTCT
-+
-FFFF@;.9BF..;-./-A=F
-@M01368:8:000000000-A3GHV:1:1103:25465:20010/1
-AAAAAAAAAAAACAACAGAC
-+
-.B/;:>---;--..;.../9
-@M01368:8:000000000-A3GHV:1:1103:25519:6301/1
-GAGACTAAGGCGAATTTCGT
-+
--B?DFFF/B/B?---.//;.
-@M01368:8:000000000-A3GHV:1:1103:6074:6549/1
-GTATCTGTCTTTGATTCCTG
-+
-A./FFFFFFFFFFFF/BFF/
-@M01368:8:000000000-A3GHV:1:1104:11572:8264/1
-TATTATTTATCGCACCTACG
-+
-B/BFFFF/BFFE?EFAFEF9
-@M01368:8:000000000-A3GHV:1:1104:11928:24197/1
-TAGGAACCAGATGTCGGATA
-+
-FFFFFFFFFFFFFFFFF@D9
-@M01368:8:000000000-A3GHV:1:1104:12288:20635/1
-TCTGTCTTTGATTCCTGCCT
-+
-;FBBFBFB///;99999BA9
-@M01368:8:000000000-A3GHV:1:1104:13832:4398/1
-ACCCAGTAGACTAGTGCGTC
-+
-9...:.9//9////;/9-.-
-@M01368:8:000000000-A3GHV:1:1104:23670:4161/1
-GGGTGATAGACCTGTGATCC
-+
-BF./AAF/BFFBBF/F/BFB
-@M01368:8:000000000-A3GHV:1:1104:24927:22253/1
-TTCCTGCCTCATCCTATTAT
-+
-//;/;9/A/;/9BFFFBFBB
-@M01368:8:000000000-A3GHV:1:1104:4705:7727/1
-CGTGATGTCTTATTTAAGGG
-+
-F?.AFFF/BFFEFFFFFEFF
-@M01368:8:000000000-A3GHV:1:1104:7756:4776/1
-GTCGCAGTATCTGTCTTTGA
-+
-FFBEFFF:9FFFFBBFFFF/
-@M01368:8:000000000-A3GHV:1:1105:11310:21761/1
-TGTCGGATACAGTTCACTTT
-+
-FFBFFFFFFFFFFFFEFFF0
-@M01368:8:000000000-A3GHV:1:1105:11578:14676/1
-TGAAGTAGGAACCAGATGTC
-+
-FF/FFFFFFFFFFFFFFFF0
-@M01368:8:000000000-A3GHV:1:1105:13575:19255/1
-TGGGCTATTTAGGTTTTATG
-+
-FF?B---;B99//9/;A//9
-@M01368:8:000000000-A3GHV:1:1105:15502:11453/1
-CTGTCTCTTATACACATCTC
-+
-FFFFEFFFFFFFFBFFFBFF
-@M01368:8:000000000-A3GHV:1:1105:16787:9771/1
-GACCTGTGATCCATCGTGAT
-+
-FFFFFFBFFFFFFFFFEFF/
-@M01368:8:000000000-A3GHV:1:1105:17564:15723/1
-TTAGGTTTTATGACCCTGAA
-+
-FFBBFBBFFFFFE1:BFFB0
-@M01368:8:000000000-A3GHV:1:1105:18108:12091/1
-GATACCTGTCTCTTATACAC
-+
-F-A.B//;BFFEF//BB///
-@M01368:8:000000000-A3GHV:1:1105:18331:16683/1
-CGCACCTACGTTCAATATTA
-+
-B.DABFFFFFFB.//:FB//
-@M01368:8:000000000-A3GHV:1:1105:20577:18421/1
-CTTTGATTCCTGCCTCATCC
-+
-FBB/:FF9BF/BF.BBFF/:
-@M01368:8:000000000-A3GHV:1:1105:21099:2895/1
-ATTCCTGCCTCATCCTATTT
-+
-BFBFFBFF.0:000BFFBF0
-@M01368:8:000000000-A3GHV:1:1105:26469:19245/1
-ACCTGTGATCCATCGTGATG
-+
-FFFFFFF/9FFFFBDEFFFF
-@M01368:8:000000000-A3GHV:1:1105:7169:17145/1
-GAACATACTTACTAAAGTGT
-+
--9@DDBFFFFFFFFBBFFF0
-@M01368:8:000000000-A3GHV:1:1106:15481:16176/1
-TGTGGGCTATTTAGGTTTTT
-+
-FE:AAFEF.BFFFFF;9FF.
-@M01368:8:000000000-A3GHV:1:1106:17389:18671/1
-GCAGTATCTGGCTTTGATTC
-+
-..;A;/B/;B//;;//BBF/
-@M01368:8:000000000-A3GHV:1:1106:22927:3863/1
-CTCCGAGCCCACGAGACTAA
-+
-//FFF-=--.99=--.9///
-@M01368:8:000000000-A3GHV:1:1106:23625:4236/1
-ATTCCTGCCTCATCCTATTA
-+
-EFFFFFFFAF///9FF/BB/
-@M01368:8:000000000-A3GHV:1:1106:26031:14826/1
-GTATGCCGTCTTCTGCTTGA
-+
-AFFFFFF;;BFE/BFFFFB:
-@M01368:8:000000000-A3GHV:1:1106:27607:15990/1
-AACTACAACCCACGGACGCA
-+
-;B.//////;...---9---
-@M01368:8:000000000-A3GHV:1:1106:6455:17124/1
-TATTATTTATCGCACCTACG
-+
-F/FFBFFFFFFADE>BFFF9
-@M01368:8:000000000-A3GHV:1:1107:14451:26748/1
-TTTAGGTTTTATGACCCTGA
-+
-FBFFFF;FFEBFBFFB./9.
-@M01368:8:000000000-A3GHV:1:1107:18714:24788/1
-TTTAGGTTTTATGACCCTGA
-+
-FFFFFFBFFFFFBBFFBBF/
-@M01368:8:000000000-A3GHV:1:1107:21355:8333/1
-CCTGAAGTAGGAACCAGATG
-+
-FFFFFFFFFFFFBFF.AA/9
-@M01368:8:000000000-A3GHV:1:1107:22864:15712/1
-ACTAAGGCGAATCTCGTATG
-+
-9DFFF/FFBF;-.:;.9A//
-@M01368:8:000000000-A3GHV:1:1107:24817:14233/1
-TCAATATTACAGGCGAACAT
-+
-FFFFFBF//:BFBACF--9/
-@M01368:8:000000000-A3GHV:1:1107:24830:14440/1
-ATCCTATTATTTATCGCACC
-+
-FFFFFFFFFFF/BFBEFFC-
-@M01368:8:000000000-A3GHV:1:1107:26478:23602/1
-CTGTCTCTTATACACCTCTC
-+
-BFFFBBFFF//B/////-;9
-@M01368:8:000000000-A3GHV:1:1108:11059:23181/1
-GACCTGTGATCCATCGTGAT
-+
-FFFFBF;BBFFBFFFFE?F/
-@M01368:8:000000000-A3GHV:1:1108:12233:13498/1
-GAACCCTGTCTCTTATACAC
-+
-EFF?EFFFF0BFFFFBBB00
-@M01368:8:000000000-A3GHV:1:1108:14328:14751/1
-GGATACCTGTCTCTTATACA
-+
-EFFFBF/BF/FFEFFBFFF/
-@M01368:8:000000000-A3GHV:1:1108:16350:22621/1
-TGGGCTATTTAGGTTTTATG
-+
-BE.99FAFFFFFFF/;EFF/
-@M01368:8:000000000-A3GHV:1:1108:21791:23272/1
-TGTGGGCTATTTAGGTTTTA
-+
-EEFEFFFBFBFBBFFFFF./
-@M01368:8:000000000-A3GHV:1:1108:23314:26089/1
-TGAAGTAGGAACCAGATGTG
-+
-BB./:BFFF/F;AA?F////
-@M01368:8:000000000-A3GHV:1:1108:25084:18127/1
-TGTGATCCATCGTGATGTCT
-+
-FF9FBFFB/F/.;FFFF/BB
-@M01368:8:000000000-A3GHV:1:1108:7626:10048/1
-TATGCCGTCTTCTGCTTGAA
-+
-AAFFFFFFAFABBFFFB/;;
-@M01368:8:000000000-A3GHV:1:1109:11270:12255/1
-AGACTAAGGCGAATCTCGTT
-+
--@DBFFFFBFD---.;BAA.
-@M01368:8:000000000-A3GHV:1:1109:19224:11655/1
-CTTATACACATCTCCGAGCC
-+
-FFB/;F/99/FBFFBDCF;B
-@M01368:8:000000000-A3GHV:1:1109:21978:9490/1
-GGCGAATCTCGTATGCAGTT
-+
-FFFFA;;;FF..9//;/;;/
-@M01368:8:000000000-A3GHV:1:1109:22910:4079/1
-GATACAGTCCACTTTAGCTA
-+
-CCDFFFFFFF0BFFFFFFF0
-@M01368:8:000000000-A3GHV:1:1109:24217:19077/1
-TGCCTCATCCTATTACTGTC
-+
-9;F./B//BBFB9////99/
-@M01368:8:000000000-A3GHV:1:1109:25237:11722/1
-AGGCGAATATCGTATGCCGT
-+
-BF/99D-;.//.../9;B-.
-@M01368:8:000000000-A3GHV:1:1109:27103:7153/1
-GACCCTGAAGTAGCTGTCTC
-+
-BBFFBFFFF///;/9B;///
-@M01368:8:000000000-A3GHV:1:1109:8763:7859/1
-GGGCTATTTAGGCTTTATGC
-+
-EDAA.A/BFFFFFFFFFEB/
-@M01368:8:000000000-A3GHV:1:1110:12808:18367/1
-AAGGGGAACGTGTGGGCCTG
-+
-BFBFFB9..AF?FFFFFFF9
-@M01368:8:000000000-A3GHV:1:1110:14751:26540/1
-GTATCTGTCTTTGATTCCTG
-+
-9-/;B9BF/9BB//B/;///
-@M01368:8:000000000-A3GHV:1:1110:15519:10008/1
-ATGACCCTGAAGTAGGAACC
-+
-FFFFFBFFF.BFFFBFBBA;
-@M01368:8:000000000-A3GHV:1:1110:16559:7507/1
-AGGGTGATAGACCTGTGATC
-+
-/99;;:.//:FFFFBBF//9
-@M01368:8:000000000-A3GHV:1:1110:16700:6843/1
-CTTATACACATCTCCGAGCC
-+
-BFFFFB/BFFBFFF/ADCAD
-@M01368:8:000000000-A3GHV:1:1110:18014:7856/1
-TGCTTGCAAAAAAAAAATAG
-+
-/BBFFF.9/FF?DA=@9./9
-@M01368:8:000000000-A3GHV:1:1110:23303:11846/1
-CTGTCTTTGATTCCTGCCTC
-+
-FBBFFFF/BBFFFFBFF9B;
-@M01368:8:000000000-A3GHV:1:1111:13195:14562/1
-ACATCTCCGAGCCCACGAGA
-+
-FFEFFFFED-DCFF?.:BFA
-@M01368:8:000000000-A3GHV:1:1111:14287:20817/1
-ATTAAAGTGTGTTAATTAAT
-+
-B//B/;/;9//9//;B/:B9
-@M01368:8:000000000-A3GHV:1:1111:23624:14516/1
-CATCGTGATGTCTTATTTAG
-+
-B/FBDE9/;99/B/BFFEF/
-@M01368:8:000000000-A3GHV:1:1111:7437:24508/1
-CACATCTCCGAGCCCACGAG
-+
-FFFFB:FFFFD=CAEEEF>-
-@M01368:8:000000000-A3GHV:1:1111:8665:25542/1
-TACGTTCAATATTACAGGCG
-+
-FFFBA/F//BFFFBF/;/A-
-@M01368:8:000000000-A3GHV:1:1111:9655:13916/1
-GTCGCAGTATCTGTCTTTGA
-+
-F/B?A@F-9BBFF//BB/9/
-@M01368:8:000000000-A3GHV:1:1112:10272:7828/1
-CGAATCTCGTATGCCGTCTT
-+
-FAD--.99;A?////:.9A.
-@M01368:8:000000000-A3GHV:1:1112:9832:16531/1
-ATGACCTTGAAGTAGGAACC
-+
-FFF/BF//9/B//9:B//..
-@M01368:8:000000000-A3GHV:1:1112:9832:6701/1
-TTTATGACCCTGAAGTAGGA
-+
-FFFFFFF/B./FB///;BF/
-@M01368:8:000000000-A3GHV:1:1113:13532:8618/1
-AGCTACCTCCAAGTGTTATG
-+
-FF/BFFB/:.BB//;FBFFF
-@M01368:8:000000000-A3GHV:1:1113:21679:18011/1
-AGACTAAGGCGAAGTTCATT
-+
-;-;AFFE;/99-9--/;///
-@M01368:8:000000000-A3GHV:1:1113:25528:14016/1
-TCGTGATGTCTTATTTAAGG
-+
-FFFEABF//99FFEFFFBFF
-@M01368:8:000000000-A3GHV:1:1113:5741:16959/1
-GGGCTATTTAGGTTTTATGA
-+
-9AD;.FFFB/B/;FBA/B//
-@M01368:8:000000000-A3GHV:1:1114:10130:11959/1
-GCAACAAACACACATCCAGA
-+
-F./B/..9.9:...//////
-@M01368:8:000000000-A3GHV:1:1114:14540:5315/1
-TGGGGGCTATTTAGGTTTTT
-+
-;A.-=A--:BBFB90;;FE.
-@M01368:8:000000000-A3GHV:1:1114:15066:16302/1
-TAATTATTGCTTGTAGGACA
-+
-F/;BFF/;/////9/////.
-@M01368:8:000000000-A3GHV:1:1114:16639:15258/1
-ACATACTTACTAAATTGTGT
-+
--9-99/B9F/://///99/;
-@M01368:8:000000000-A3GHV:1:1114:2404:13066/1
-ATCTGTCTATTATACACATC
-+
-FFFFFFFFFFFFFFFFEFF0
diff --git a/tools/trim_galore/test-data/paired_hardtrimmed3_pair2_.fastq.gz b/tools/trim_galore/test-data/paired_hardtrimmed3_pair2_.fastq.gz
new file mode 100644
index 0000000000..68acfdf8b0
Binary files /dev/null and b/tools/trim_galore/test-data/paired_hardtrimmed3_pair2_.fastq.gz differ
diff --git a/tools/trim_galore/test-data/paired_hardtrimmed3_pair2_.fastqsanger b/tools/trim_galore/test-data/paired_hardtrimmed3_pair2_.fastqsanger
deleted file mode 100644
index c0ee2935c2..0000000000
--- a/tools/trim_galore/test-data/paired_hardtrimmed3_pair2_.fastqsanger
+++ /dev/null
@@ -1,396 +0,0 @@
-@M01368:8:000000000-A3GHV:1:1101:6911:8255/1
-TCGCACCTACGTTCAATATT
-+
-FFEFE;DFFFDFBFF/9BFB
-@M01368:8:000000000-A3GHV:1:1101:14518:9998/1
-GACCTGTGATCCATCGTGAT
-+
-FF//;BFFFFFBF/9D:A//
-@M01368:8:000000000-A3GHV:1:1101:18422:19051/1
-TATTATTTATCGCACCTACG
-+
-FFEFBFFFFFFFFFF:FFF.
-@M01368:8:000000000-A3GHV:1:1101:25545:21098/1
-TTTAAGGGGAACGGGTGGGG
-+
-FBFFFFFFBF..A..;@B--
-@M01368:8:000000000-A3GHV:1:1101:5446:12248/1
-AAGGGGAACGTGTGGGCTAT
-+
-FFFBFFFFFFEFFFFFEFFF
-@M01368:8:000000000-A3GHV:1:1101:5861:6452/1
-TGATCCATCGTGATGTCTTT
-+
-FFFFFBFFBD.AFFFFFFF0
-@M01368:8:000000000-A3GHV:1:1102:10403:6021/1
-ACCCTATGTCGCAGTATCTG
-+
-..9..9/9;BEF;A:9/BFF
-@M01368:8:000000000-A3GHV:1:1102:10677:23253/1
-AATTCTGTCTCTTATACACA
-+
-F0FFFFFFFFFFFFFFFFF:
-@M01368:8:000000000-A3GHV:1:1102:13809:1733/1
-GTCTCTTATACACATCTCCG
-+
-FBBBFFFFFBFF0BFBF9F.
-@M01368:8:000000000-A3GHV:1:1102:17584:10050/1
-AATCTCGTATGCCGTCTTCT
-+
-FF;.BF9ADF/9;A;DFFF0
-@M01368:8:000000000-A3GHV:1:1102:18842:24844/1
-TCTGTCTTTGATTCCTGCCT
-+
-BBBFBFBFFF//BFB/BFF/
-@M01368:8:000000000-A3GHV:1:1102:21788:11027/1
-ACTAAAGTGTGTTAATTCTG
-+
-FBFF0FFBBBBFBFBFFFF0
-@M01368:8:000000000-A3GHV:1:1102:5728:8777/1
-CCTGAAGTAGGAACCAGATG
-+
-FFFFBBFFFFF/FFEFFFBB
-@M01368:8:000000000-A3GHV:1:1102:5983:21093/1
-TATTATTTATCGCACCTACG
-+
-F0BFFFBBFF0EFFFFFFFA
-@M01368:8:000000000-A3GHV:1:1102:7765:18353/1
-CCTATGTCGCAGTATCTGTC
-+
-.::FBFBFFE-A9:BFFFF0
-@M01368:8:000000000-A3GHV:1:1103:10405:17879/1
-TATTATTTATCGCACCTACG
-+
-FBFFFFFFFFFFFFFFFFF?
-@M01368:8:000000000-A3GHV:1:1103:13493:8205/1
-GATTCCTGCCTCATCCTATT
-+
-FFFFFFFFBFBBBFFFFEFF
-@M01368:8:000000000-A3GHV:1:1103:14060:21273/1
-TATTACTGTCTCTTATACAC
-+
-:FFFBB/BFFFFFBFFFFB/
-@M01368:8:000000000-A3GHV:1:1103:14528:12993/1
-TCGCAGTATCTGTCTTTGAT
-+
-BFEA-A.B//9F9F//;BBB
-@M01368:8:000000000-A3GHV:1:1103:17657:20856/1
-TGCCTCATCCTATTATTTAT
-+
-FFFFFFFFFFF/FFFFBFF9
-@M01368:8:000000000-A3GHV:1:1103:23099:3794/1
-GCGAATCTCGGATGCCGTCT
-+
-FFFF@;.9BF..;-./-A=F
-@M01368:8:000000000-A3GHV:1:1103:25465:20010/1
-AAAAAAAAAAAACAACAGAC
-+
-.B/;:>---;--..;.../9
-@M01368:8:000000000-A3GHV:1:1103:25519:6301/1
-GAGACTAAGGCGAATTTCGT
-+
--B?DFFF/B/B?---.//;.
-@M01368:8:000000000-A3GHV:1:1103:6074:6549/1
-GTATCTGTCTTTGATTCCTG
-+
-A./FFFFFFFFFFFF/BFF/
-@M01368:8:000000000-A3GHV:1:1104:11572:8264/1
-TATTATTTATCGCACCTACG
-+
-B/BFFFF/BFFE?EFAFEF9
-@M01368:8:000000000-A3GHV:1:1104:11928:24197/1
-TAGGAACCAGATGTCGGATA
-+
-FFFFFFFFFFFFFFFFF@D9
-@M01368:8:000000000-A3GHV:1:1104:12288:20635/1
-TCTGTCTTTGATTCCTGCCT
-+
-;FBBFBFB///;99999BA9
-@M01368:8:000000000-A3GHV:1:1104:13832:4398/1
-ACCCAGTAGACTAGTGCGTC
-+
-9...:.9//9////;/9-.-
-@M01368:8:000000000-A3GHV:1:1104:23670:4161/1
-GGGTGATAGACCTGTGATCC
-+
-BF./AAF/BFFBBF/F/BFB
-@M01368:8:000000000-A3GHV:1:1104:24927:22253/1
-TTCCTGCCTCATCCTATTAT
-+
-//;/;9/A/;/9BFFFBFBB
-@M01368:8:000000000-A3GHV:1:1104:4705:7727/1
-CGTGATGTCTTATTTAAGGG
-+
-F?.AFFF/BFFEFFFFFEFF
-@M01368:8:000000000-A3GHV:1:1104:7756:4776/1
-GTCGCAGTATCTGTCTTTGA
-+
-FFBEFFF:9FFFFBBFFFF/
-@M01368:8:000000000-A3GHV:1:1105:11310:21761/1
-TGTCGGATACAGTTCACTTT
-+
-FFBFFFFFFFFFFFFEFFF0
-@M01368:8:000000000-A3GHV:1:1105:11578:14676/1
-TGAAGTAGGAACCAGATGTC
-+
-FF/FFFFFFFFFFFFFFFF0
-@M01368:8:000000000-A3GHV:1:1105:13575:19255/1
-TGGGCTATTTAGGTTTTATG
-+
-FF?B---;B99//9/;A//9
-@M01368:8:000000000-A3GHV:1:1105:15502:11453/1
-CTGTCTCTTATACACATCTC
-+
-FFFFEFFFFFFFFBFFFBFF
-@M01368:8:000000000-A3GHV:1:1105:16787:9771/1
-GACCTGTGATCCATCGTGAT
-+
-FFFFFFBFFFFFFFFFEFF/
-@M01368:8:000000000-A3GHV:1:1105:17564:15723/1
-TTAGGTTTTATGACCCTGAA
-+
-FFBBFBBFFFFFE1:BFFB0
-@M01368:8:000000000-A3GHV:1:1105:18108:12091/1
-GATACCTGTCTCTTATACAC
-+
-F-A.B//;BFFEF//BB///
-@M01368:8:000000000-A3GHV:1:1105:18331:16683/1
-CGCACCTACGTTCAATATTA
-+
-B.DABFFFFFFB.//:FB//
-@M01368:8:000000000-A3GHV:1:1105:20577:18421/1
-CTTTGATTCCTGCCTCATCC
-+
-FBB/:FF9BF/BF.BBFF/:
-@M01368:8:000000000-A3GHV:1:1105:21099:2895/1
-ATTCCTGCCTCATCCTATTT
-+
-BFBFFBFF.0:000BFFBF0
-@M01368:8:000000000-A3GHV:1:1105:26469:19245/1
-ACCTGTGATCCATCGTGATG
-+
-FFFFFFF/9FFFFBDEFFFF
-@M01368:8:000000000-A3GHV:1:1105:7169:17145/1
-GAACATACTTACTAAAGTGT
-+
--9@DDBFFFFFFFFBBFFF0
-@M01368:8:000000000-A3GHV:1:1106:15481:16176/1
-TGTGGGCTATTTAGGTTTTT
-+
-FE:AAFEF.BFFFFF;9FF.
-@M01368:8:000000000-A3GHV:1:1106:17389:18671/1
-GCAGTATCTGGCTTTGATTC
-+
-..;A;/B/;B//;;//BBF/
-@M01368:8:000000000-A3GHV:1:1106:22927:3863/1
-CTCCGAGCCCACGAGACTAA
-+
-//FFF-=--.99=--.9///
-@M01368:8:000000000-A3GHV:1:1106:23625:4236/1
-ATTCCTGCCTCATCCTATTA
-+
-EFFFFFFFAF///9FF/BB/
-@M01368:8:000000000-A3GHV:1:1106:26031:14826/1
-GTATGCCGTCTTCTGCTTGA
-+
-AFFFFFF;;BFE/BFFFFB:
-@M01368:8:000000000-A3GHV:1:1106:27607:15990/1
-AACTACAACCCACGGACGCA
-+
-;B.//////;...---9---
-@M01368:8:000000000-A3GHV:1:1106:6455:17124/1
-TATTATTTATCGCACCTACG
-+
-F/FFBFFFFFFADE>BFFF9
-@M01368:8:000000000-A3GHV:1:1107:14451:26748/1
-TTTAGGTTTTATGACCCTGA
-+
-FBFFFF;FFEBFBFFB./9.
-@M01368:8:000000000-A3GHV:1:1107:18714:24788/1
-TTTAGGTTTTATGACCCTGA
-+
-FFFFFFBFFFFFBBFFBBF/
-@M01368:8:000000000-A3GHV:1:1107:21355:8333/1
-CCTGAAGTAGGAACCAGATG
-+
-FFFFFFFFFFFFBFF.AA/9
-@M01368:8:000000000-A3GHV:1:1107:22864:15712/1
-ACTAAGGCGAATCTCGTATG
-+
-9DFFF/FFBF;-.:;.9A//
-@M01368:8:000000000-A3GHV:1:1107:24817:14233/1
-TCAATATTACAGGCGAACAT
-+
-FFFFFBF//:BFBACF--9/
-@M01368:8:000000000-A3GHV:1:1107:24830:14440/1
-ATCCTATTATTTATCGCACC
-+
-FFFFFFFFFFF/BFBEFFC-
-@M01368:8:000000000-A3GHV:1:1107:26478:23602/1
-CTGTCTCTTATACACCTCTC
-+
-BFFFBBFFF//B/////-;9
-@M01368:8:000000000-A3GHV:1:1108:11059:23181/1
-GACCTGTGATCCATCGTGAT
-+
-FFFFBF;BBFFBFFFFE?F/
-@M01368:8:000000000-A3GHV:1:1108:12233:13498/1
-GAACCCTGTCTCTTATACAC
-+
-EFF?EFFFF0BFFFFBBB00
-@M01368:8:000000000-A3GHV:1:1108:14328:14751/1
-GGATACCTGTCTCTTATACA
-+
-EFFFBF/BF/FFEFFBFFF/
-@M01368:8:000000000-A3GHV:1:1108:16350:22621/1
-TGGGCTATTTAGGTTTTATG
-+
-BE.99FAFFFFFFF/;EFF/
-@M01368:8:000000000-A3GHV:1:1108:21791:23272/1
-TGTGGGCTATTTAGGTTTTA
-+
-EEFEFFFBFBFBBFFFFF./
-@M01368:8:000000000-A3GHV:1:1108:23314:26089/1
-TGAAGTAGGAACCAGATGTG
-+
-BB./:BFFF/F;AA?F////
-@M01368:8:000000000-A3GHV:1:1108:25084:18127/1
-TGTGATCCATCGTGATGTCT
-+
-FF9FBFFB/F/.;FFFF/BB
-@M01368:8:000000000-A3GHV:1:1108:7626:10048/1
-TATGCCGTCTTCTGCTTGAA
-+
-AAFFFFFFAFABBFFFB/;;
-@M01368:8:000000000-A3GHV:1:1109:11270:12255/1
-AGACTAAGGCGAATCTCGTT
-+
--@DBFFFFBFD---.;BAA.
-@M01368:8:000000000-A3GHV:1:1109:19224:11655/1
-CTTATACACATCTCCGAGCC
-+
-FFB/;F/99/FBFFBDCF;B
-@M01368:8:000000000-A3GHV:1:1109:21978:9490/1
-GGCGAATCTCGTATGCAGTT
-+
-FFFFA;;;FF..9//;/;;/
-@M01368:8:000000000-A3GHV:1:1109:22910:4079/1
-GATACAGTCCACTTTAGCTA
-+
-CCDFFFFFFF0BFFFFFFF0
-@M01368:8:000000000-A3GHV:1:1109:24217:19077/1
-TGCCTCATCCTATTACTGTC
-+
-9;F./B//BBFB9////99/
-@M01368:8:000000000-A3GHV:1:1109:25237:11722/1
-AGGCGAATATCGTATGCCGT
-+
-BF/99D-;.//.../9;B-.
-@M01368:8:000000000-A3GHV:1:1109:27103:7153/1
-GACCCTGAAGTAGCTGTCTC
-+
-BBFFBFFFF///;/9B;///
-@M01368:8:000000000-A3GHV:1:1109:8763:7859/1
-GGGCTATTTAGGCTTTATGC
-+
-EDAA.A/BFFFFFFFFFEB/
-@M01368:8:000000000-A3GHV:1:1110:12808:18367/1
-AAGGGGAACGTGTGGGCCTG
-+
-BFBFFB9..AF?FFFFFFF9
-@M01368:8:000000000-A3GHV:1:1110:14751:26540/1
-GTATCTGTCTTTGATTCCTG
-+
-9-/;B9BF/9BB//B/;///
-@M01368:8:000000000-A3GHV:1:1110:15519:10008/1
-ATGACCCTGAAGTAGGAACC
-+
-FFFFFBFFF.BFFFBFBBA;
-@M01368:8:000000000-A3GHV:1:1110:16559:7507/1
-AGGGTGATAGACCTGTGATC
-+
-/99;;:.//:FFFFBBF//9
-@M01368:8:000000000-A3GHV:1:1110:16700:6843/1
-CTTATACACATCTCCGAGCC
-+
-BFFFFB/BFFBFFF/ADCAD
-@M01368:8:000000000-A3GHV:1:1110:18014:7856/1
-TGCTTGCAAAAAAAAAATAG
-+
-/BBFFF.9/FF?DA=@9./9
-@M01368:8:000000000-A3GHV:1:1110:23303:11846/1
-CTGTCTTTGATTCCTGCCTC
-+
-FBBFFFF/BBFFFFBFF9B;
-@M01368:8:000000000-A3GHV:1:1111:13195:14562/1
-ACATCTCCGAGCCCACGAGA
-+
-FFEFFFFED-DCFF?.:BFA
-@M01368:8:000000000-A3GHV:1:1111:14287:20817/1
-ATTAAAGTGTGTTAATTAAT
-+
-B//B/;/;9//9//;B/:B9
-@M01368:8:000000000-A3GHV:1:1111:23624:14516/1
-CATCGTGATGTCTTATTTAG
-+
-B/FBDE9/;99/B/BFFEF/
-@M01368:8:000000000-A3GHV:1:1111:7437:24508/1
-CACATCTCCGAGCCCACGAG
-+
-FFFFB:FFFFD=CAEEEF>-
-@M01368:8:000000000-A3GHV:1:1111:8665:25542/1
-TACGTTCAATATTACAGGCG
-+
-FFFBA/F//BFFFBF/;/A-
-@M01368:8:000000000-A3GHV:1:1111:9655:13916/1
-GTCGCAGTATCTGTCTTTGA
-+
-F/B?A@F-9BBFF//BB/9/
-@M01368:8:000000000-A3GHV:1:1112:10272:7828/1
-CGAATCTCGTATGCCGTCTT
-+
-FAD--.99;A?////:.9A.
-@M01368:8:000000000-A3GHV:1:1112:9832:16531/1
-ATGACCTTGAAGTAGGAACC
-+
-FFF/BF//9/B//9:B//..
-@M01368:8:000000000-A3GHV:1:1112:9832:6701/1
-TTTATGACCCTGAAGTAGGA
-+
-FFFFFFF/B./FB///;BF/
-@M01368:8:000000000-A3GHV:1:1113:13532:8618/1
-AGCTACCTCCAAGTGTTATG
-+
-FF/BFFB/:.BB//;FBFFF
-@M01368:8:000000000-A3GHV:1:1113:21679:18011/1
-AGACTAAGGCGAAGTTCATT
-+
-;-;AFFE;/99-9--/;///
-@M01368:8:000000000-A3GHV:1:1113:25528:14016/1
-TCGTGATGTCTTATTTAAGG
-+
-FFFEABF//99FFEFFFBFF
-@M01368:8:000000000-A3GHV:1:1113:5741:16959/1
-GGGCTATTTAGGTTTTATGA
-+
-9AD;.FFFB/B/;FBA/B//
-@M01368:8:000000000-A3GHV:1:1114:10130:11959/1
-GCAACAAACACACATCCAGA
-+
-F./B/..9.9:...//////
-@M01368:8:000000000-A3GHV:1:1114:14540:5315/1
-TGGGGGCTATTTAGGTTTTT
-+
-;A.-=A--:BBFB90;;FE.
-@M01368:8:000000000-A3GHV:1:1114:15066:16302/1
-TAATTATTGCTTGTAGGACA
-+
-F/;BFF/;/////9/////.
-@M01368:8:000000000-A3GHV:1:1114:16639:15258/1
-ACATACTTACTAAATTGTGT
-+
--9-99/B9F/://///99/;
-@M01368:8:000000000-A3GHV:1:1114:2404:13066/1
-ATCTGTCTATTATACACATC
-+
-FFFFFFFFFFFFFFFFEFF0
diff --git a/tools/trim_galore/test-data/paired_hardtrimmed5_pair1_.fastq.gz b/tools/trim_galore/test-data/paired_hardtrimmed5_pair1_.fastq.gz
new file mode 100644
index 0000000000..6d12db1c5a
Binary files /dev/null and b/tools/trim_galore/test-data/paired_hardtrimmed5_pair1_.fastq.gz differ
diff --git a/tools/trim_galore/test-data/paired_hardtrimmed5_pair1_.fastqsanger b/tools/trim_galore/test-data/paired_hardtrimmed5_pair1_.fastqsanger
deleted file mode 100644
index b1d8bc7b90..0000000000
--- a/tools/trim_galore/test-data/paired_hardtrimmed5_pair1_.fastqsanger
+++ /dev/null
@@ -1,396 +0,0 @@
-@M01368:8:000000000-A3GHV:1:1101:6911:8255/1
-ATCTGGTTCCTACTTCAGGG
-+
-BCCCCFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1101:14518:9998/1
-GTTATTATTATGTCCTACAA
-+
-AAAAAFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1101:18422:19051/1
-GTATCCGACATCTGGTTCCT
-+
-CCCCCFDDDDDFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1101:25545:21098/1
-ATTAATTAACACACTTTAGT
-+
-33AA?DFD5BDFGGGFEBDG
-@M01368:8:000000000-A3GHV:1:1101:5446:12248/1
-AATTAACACACTTTAGTAAG
-+
-CCCCDFFFFCCFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1101:5861:6452/1
-ATTATGTCCTACAAGCATTA
-+
-ABCCCFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1102:10403:6021/1
-CGCTCCGGGCCCATAACACT
-+
->A@AAAAD2ADDFFGGGGGF
-@M01368:8:000000000-A3GHV:1:1102:10677:23253/1
-CCTTAAATAAGACATCACGA
-+
-ABBBBFFFFFFFGGGGGGGC
-@M01368:8:000000000-A3GHV:1:1102:13809:1733/1
-ATATTGAACGTAGGTGCGAT
-+
-BCCBCFFFFFBFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1102:17584:10050/1
-ATCAAAGACAGATACTGCGA
-+
-CCCCCFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1102:18842:24844/1
-CACTTGGGGGTAGCTAAAGT
-+
-BBBCBFF@CCBBGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1102:21788:11027/1
-GCCCACACGTTCCCCTTAAA
-+
-ABCCBCCBBFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1102:5728:8777/1
-ATATTGAACGTAGGTGCGAT
-+
-BBBBBFFFFFAFGG5GGGGG
-@M01368:8:000000000-A3GHV:1:1102:5983:21093/1
-GTATCCGACATCTGGTTCCT
-+
-AAABCFCABBCFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1102:7765:18353/1
-CTCCGGGCCCATAACACTTG
-+
-CCDCCCCCCBCFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1103:10405:17879/1
-GTATCCGACATCTGGTTCCT
-+
-CBCCCFCCCCCFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1103:13493:8205/1
-GTAGCTAAAGTGAACTGTAT
-+
-CCCCCFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1103:14060:21273/1
-GTATCCGACATCTGGTTCCT
-+
-AAABAFCCCCCFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1103:14528:12993/1
-GCCCATAACACTTGGGGGTA
-+
-BBBBBFFFBDFFGFBCG2EG
-@M01368:8:000000000-A3GHV:1:1103:17657:20856/1
-AAAGCGAACTGTATCCGACA
-+
-ABB?AABBBBFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1103:23099:3794/1
-AAGACAGATACTGCGACATA
-+
-BBBBBFFFFFFFGGEEGGFG
-@M01368:8:000000000-A3GHV:1:1103:25465:20010/1
-GACATAGGGTGCTCCGGCTC
-+
-ABBBBFFFFBAAGGGFEFGG
-@M01368:8:000000000-A3GHV:1:1103:25519:6301/1
-AAATAAGACATCACGATGGA
-+
-3A3AAFFFFBFFGGG2CF?4
-@M01368:8:000000000-A3GHV:1:1103:6074:6549/1
-TAACACTTGGGGGTAGCTAA
-+
-CCCBCFFFFFCCGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1104:11572:8264/1
-GTATCCGACATCTGGTTCCT
-+
-BABCBFBACCCFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1104:11928:24197/1
-ACGTAGGTGCGATAAATAAT
-+
-CDDCCFCFFFBCGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1104:12288:20635/1
-CACTTGGGGGTAGCTAAAGT
-+
-ABA?AFBAB2>AFGGGGFFG
-@M01368:8:000000000-A3GHV:1:1104:13832:4398/1
-CACGATGGATCACAGGTCTA
-+
-AAABABCAFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1104:23670:4161/1
-ATTCAATTGTTATTATTATG
-+
-AAAABFFFFFFFGGGGGGGC
-@M01368:8:000000000-A3GHV:1:1104:24927:22253/1
-AGCTAAAGTGAACTGTATCC
-+
-AAA?AFFF3DFFFGGBGFF3
-@M01368:8:000000000-A3GHV:1:1104:4705:7727/1
-CTACAAGCATTAATTAATTA
-+
-BBBBBFFBFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1104:7756:4776/1
-GGCCCATAACACTTGGGGGT
-+
-CCCCCCFFFFFFGGGGGGGE
-@M01368:8:000000000-A3GHV:1:1105:11310:21761/1
-ATAAATAATAGGATGAGGCA
-+
-CCCCBFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1105:11578:14676/1
-ATTGAACGTAGGTGCGATAA
-+
-CDDDDFFFBFFCGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1105:13575:19255/1
-TTAGTAAGTATGTTCGCCTG
-+
-AA1A1DFFFFFBGG3A111F
-@M01368:8:000000000-A3GHV:1:1105:15502:11453/1
-GTCCTACAAGCATTAATTAA
-+
-A3AAAFFFFFCFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1105:16787:9771/1
-GTTATTATTATGTCCTACAA
-+
-AABCCFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1105:17564:15723/1
-TATGCTCGCCTGTAATATTG
-+
-EDCCDFFCEEDCGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1105:18108:12091/1
-TAATAGGATGAGGCAGGAAT
-+
-CCCCCFBFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1105:18331:16683/1
-TCTGGTTCCTACTTCAGGGT
-+
-BABA?DFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1105:20577:18421/1
-GGGGGTAGCTAAAGTGAACT
-+
-ABB@3>AAFFFFFEAGGGFG
-@M01368:8:000000000-A3GHV:1:1105:21099:2895/1
-TAGCTAAAGTGAACTGTATC
-+
-CBCBBFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1105:26469:19245/1
-TTATGATTATGTCCTACAAG
-+
-BAAAAFFFBFFFGGGGGGFF
-@M01368:8:000000000-A3GHV:1:1105:7169:17145/1
-AACCTAAATAGCCCACACGT
-+
-CCCCCFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1106:15481:16176/1
-CTTTAGTAAGTATGTTCGCC
-+
-ABBBBFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1106:17389:18671/1
-CCATAACACTTGGGGGTAGC
-+
-3>>A@FFFFFFFGGGFEECF
-@M01368:8:000000000-A3GHV:1:1106:22927:3863/1
-GTATCCGACATCTGGTTCCT
-+
-A>ABAFA?D@AFGGGGFGGG
-@M01368:8:000000000-A3GHV:1:1106:23625:4236/1
-TAGCTAAAGTGAACTGTATC
-+
-@?AAAFFBFBFFFGGFGFGG
-@M01368:8:000000000-A3GHV:1:1106:26031:14826/1
-GATCACAGGTCTATCACCCT
-+
-ABBAAFFF@FFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1106:27607:15990/1
-GACAGATACTGCGACATAGG
-+
-BBBBBFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1106:6455:17124/1
-GTATCCGACATCTGGTTCCT
-+
-BBBAAFABDBBFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1107:14451:26748/1
-GTATGTTCGCCTGTAATATT
-+
-BABBBFFDABBBGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1107:18714:24788/1
-GTATGTTCGCCTGTAATATT
-+
-BBBCCFFFDEDEGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1107:21355:8333/1
-ATATTGAACGTAGGTGCGAT
-+
-ABCBBFFFFFAFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1107:22864:15712/1
-AATAAGACATCACGATGGAT
-+
-BBBBBFBBFFFFGGGGCEGG
-@M01368:8:000000000-A3GHV:1:1107:24817:14233/1
-CTTCAGGGTCATAAAACCTA
-+
-3AABABFFBFFFGCFGGGGG
-@M01368:8:000000000-A3GHV:1:1107:24830:14440/1
-AACTGTATCCGACATCTGGT
-+
-ABBBA@FFFFBBGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1107:26478:23602/1
-GCTAAAGTGAACTGTATCCG
-+
-1AAAAF1B13BBBEGGGFGG
-@M01368:8:000000000-A3GHV:1:1108:11059:23181/1
-GTTATTATTATGTCCTACAA
-+
-CCCCCFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1108:12233:13498/1
-TAGGTGCGATAAATAATAGG
-+
-BCCBAFFBCCCCGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1108:14328:14751/1
-ATAATAGGATGAGGCAGGAA
-+
-BCCCBFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1108:16350:22621/1
-TTAGTAAGTATGTTCGCCTG
-+
-AAAACFFFFFFFGGGFGGGG
-@M01368:8:000000000-A3GHV:1:1108:21791:23272/1
-CTTTAGTAAGTATGTTCGCC
-+
-3ABBBFFFFF@FGGGGGGGC
-@M01368:8:000000000-A3GHV:1:1108:23314:26089/1
-ATTGAACGTAGGTGCGATAA
-+
-BBBBBFFF?FFAGFBFGGEG
-@M01368:8:000000000-A3GHV:1:1108:25084:18127/1
-TTATTATGTCCTACAAGCAT
-+
-CCBBCFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1108:7626:10048/1
-ATCAAAGACAGATACTGCGA
-+
-CCCCCFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1109:11270:12255/1
-AATCAAAGACAGATACTGCG
-+
-CCCCCFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1109:19224:11655/1
-AAATAGCCCACACGTTCCCC
-+
-BBB@A4FFFBBBGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1109:21978:9490/1
-GGGTCATAAAACCTAAATAG
-+
-?A3AA?DFFFFFGGGGGGGF
-@M01368:8:000000000-A3GHV:1:1109:22910:4079/1
-TAATAGGATGAGGCAGGAAT
-+
-CCCCCFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1109:24217:19077/1
-AAAGTGAACTGTATCCGACA
-+
->AAA3BDFFFFDGBFFEEGG
-@M01368:8:000000000-A3GHV:1:1109:25237:11722/1
-CCTGTAATATTGAACGTAGG
-+
-AABBBFFFFFBBF5BGGFGA
-@M01368:8:000000000-A3GHV:1:1109:27103:7153/1
-GTAATATTGAACGTAGGTGC
-+
-33>A?FFFDFBFGCG4EGFG
-@M01368:8:000000000-A3GHV:1:1109:8763:7859/1
-TAGTAAGTATGTTCGCCTGT
-+
-BABAADFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1110:12808:18367/1
-AATTAACACACTTTAGTAAG
-+
-BBBBBFFFFBBFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1110:14751:26540/1
-TAACACTTGGGGGTAGCTAA
-+
-AAA?1CFFFFADGEEGFGB1
-@M01368:8:000000000-A3GHV:1:1110:15519:10008/1
-CTGTAATATTGAACGTAGGT
-+
-BBBBBFFFFFFFGGGGGGCB
-@M01368:8:000000000-A3GHV:1:1110:16559:7507/1
-CATTCAATTGTTATTATTAT
-+
-ABBBAFFFFFFFFGGGGGGG
-@M01368:8:000000000-A3GHV:1:1110:16700:6843/1
-AAATAATAGGATGAGGCAGG
-+
-BBBBBFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1110:18014:7856/1
-ATAAATAATAGGATGAGGCA
-+
-AABBBFFFFFFFCGGGCGGG
-@M01368:8:000000000-A3GHV:1:1110:23303:11846/1
-ACTTGGGGGTAGCTAAAGTG
-+
-3ABB?FAB?DAAGGGFBGGB
-@M01368:8:000000000-A3GHV:1:1111:13195:14562/1
-ATCAAAGACAGATACTGCGA
-+
-ABAAAFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1111:14287:20817/1
-GCCAACACGTTCCCCTTAAA
-+
->>A3>AAAACFFFGFGFGGG
-@M01368:8:000000000-A3GHV:1:1111:23624:14516/1
-GTCCTACAAGCATTAATTAA
-+
-3>3AAFFFFFAFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1111:7437:24508/1
-ACACACTTTAGTAAGTATGT
-+
-CBCCDCFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1111:8665:25542/1
-TCCTACTTCAGGGTCATAAA
-+
-AA3AAFFFFFFFGGGFGFFG
-@M01368:8:000000000-A3GHV:1:1111:9655:13916/1
-GGCCCATAACACTTGGGGGT
-+
-1>>1AAFFFFFBGGFE1EEE
-@M01368:8:000000000-A3GHV:1:1112:10272:7828/1
-GTATCCGACATCTGGTTCCT
-+
-AAABABBBBBBFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1112:9832:16531/1
-CTGTAATATTGAACGTAGGT
-+
-AABABFFFFFFFF4GGGGEB
-@M01368:8:000000000-A3GHV:1:1112:9832:6701/1
-CGCCTGTAATATTGAACGTA
-+
-AABBBBFFFFFFGGGFGGGG
-@M01368:8:000000000-A3GHV:1:1113:13532:8618/1
-GGAATCAAAGACAGATACTG
-+
-AA?AAFFFFFFFGGGGGFBF
-@M01368:8:000000000-A3GHV:1:1113:21679:18011/1
-GATCACAGGTCTATCACCCT
-+
-AA>1>11F1D1DDFFFFGGG
-@M01368:8:000000000-A3GHV:1:1113:25528:14016/1
-CCTACAAGCATTAATTAATT
-+
->AAAAFFFBFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1113:5741:16959/1
-TAGTAAGTATGTTCGCCTGT
-+
-ABBBBFFFFFFFGGGFGGGG
-@M01368:8:000000000-A3GHV:1:1114:10130:11959/1
-CGTTCCCCTTAAATAAGACA
-+
->A33>FFFFFF@FFFGGGGF
-@M01368:8:000000000-A3GHV:1:1114:14540:5315/1
-CTTTAGTAAGTATGTTCGCC
-+
-AABCCFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1114:15066:16302/1
-TAAATAAGACATCACGATGG
-+
-BBBBAFFBDFBFBGGGGGFG
-@M01368:8:000000000-A3GHV:1:1114:16639:15258/1
-CCTAAATAGCCCACACGTTC
-+
-11>>ABFFFFFFGGCGC1BB
-@M01368:8:000000000-A3GHV:1:1114:2404:13066/1
-TCCTACAAGCATTAATTAAT
-+
-CCCCCFFFFCFFGGGGGGGG
diff --git a/tools/trim_galore/test-data/paired_hardtrimmed5_pair2_.fastq.gz b/tools/trim_galore/test-data/paired_hardtrimmed5_pair2_.fastq.gz
new file mode 100644
index 0000000000..f8295bdb02
Binary files /dev/null and b/tools/trim_galore/test-data/paired_hardtrimmed5_pair2_.fastq.gz differ
diff --git a/tools/trim_galore/test-data/paired_hardtrimmed5_pair2_.fastqsanger b/tools/trim_galore/test-data/paired_hardtrimmed5_pair2_.fastqsanger
deleted file mode 100644
index b1d8bc7b90..0000000000
--- a/tools/trim_galore/test-data/paired_hardtrimmed5_pair2_.fastqsanger
+++ /dev/null
@@ -1,396 +0,0 @@
-@M01368:8:000000000-A3GHV:1:1101:6911:8255/1
-ATCTGGTTCCTACTTCAGGG
-+
-BCCCCFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1101:14518:9998/1
-GTTATTATTATGTCCTACAA
-+
-AAAAAFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1101:18422:19051/1
-GTATCCGACATCTGGTTCCT
-+
-CCCCCFDDDDDFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1101:25545:21098/1
-ATTAATTAACACACTTTAGT
-+
-33AA?DFD5BDFGGGFEBDG
-@M01368:8:000000000-A3GHV:1:1101:5446:12248/1
-AATTAACACACTTTAGTAAG
-+
-CCCCDFFFFCCFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1101:5861:6452/1
-ATTATGTCCTACAAGCATTA
-+
-ABCCCFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1102:10403:6021/1
-CGCTCCGGGCCCATAACACT
-+
->A@AAAAD2ADDFFGGGGGF
-@M01368:8:000000000-A3GHV:1:1102:10677:23253/1
-CCTTAAATAAGACATCACGA
-+
-ABBBBFFFFFFFGGGGGGGC
-@M01368:8:000000000-A3GHV:1:1102:13809:1733/1
-ATATTGAACGTAGGTGCGAT
-+
-BCCBCFFFFFBFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1102:17584:10050/1
-ATCAAAGACAGATACTGCGA
-+
-CCCCCFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1102:18842:24844/1
-CACTTGGGGGTAGCTAAAGT
-+
-BBBCBFF@CCBBGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1102:21788:11027/1
-GCCCACACGTTCCCCTTAAA
-+
-ABCCBCCBBFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1102:5728:8777/1
-ATATTGAACGTAGGTGCGAT
-+
-BBBBBFFFFFAFGG5GGGGG
-@M01368:8:000000000-A3GHV:1:1102:5983:21093/1
-GTATCCGACATCTGGTTCCT
-+
-AAABCFCABBCFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1102:7765:18353/1
-CTCCGGGCCCATAACACTTG
-+
-CCDCCCCCCBCFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1103:10405:17879/1
-GTATCCGACATCTGGTTCCT
-+
-CBCCCFCCCCCFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1103:13493:8205/1
-GTAGCTAAAGTGAACTGTAT
-+
-CCCCCFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1103:14060:21273/1
-GTATCCGACATCTGGTTCCT
-+
-AAABAFCCCCCFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1103:14528:12993/1
-GCCCATAACACTTGGGGGTA
-+
-BBBBBFFFBDFFGFBCG2EG
-@M01368:8:000000000-A3GHV:1:1103:17657:20856/1
-AAAGCGAACTGTATCCGACA
-+
-ABB?AABBBBFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1103:23099:3794/1
-AAGACAGATACTGCGACATA
-+
-BBBBBFFFFFFFGGEEGGFG
-@M01368:8:000000000-A3GHV:1:1103:25465:20010/1
-GACATAGGGTGCTCCGGCTC
-+
-ABBBBFFFFBAAGGGFEFGG
-@M01368:8:000000000-A3GHV:1:1103:25519:6301/1
-AAATAAGACATCACGATGGA
-+
-3A3AAFFFFBFFGGG2CF?4
-@M01368:8:000000000-A3GHV:1:1103:6074:6549/1
-TAACACTTGGGGGTAGCTAA
-+
-CCCBCFFFFFCCGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1104:11572:8264/1
-GTATCCGACATCTGGTTCCT
-+
-BABCBFBACCCFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1104:11928:24197/1
-ACGTAGGTGCGATAAATAAT
-+
-CDDCCFCFFFBCGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1104:12288:20635/1
-CACTTGGGGGTAGCTAAAGT
-+
-ABA?AFBAB2>AFGGGGFFG
-@M01368:8:000000000-A3GHV:1:1104:13832:4398/1
-CACGATGGATCACAGGTCTA
-+
-AAABABCAFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1104:23670:4161/1
-ATTCAATTGTTATTATTATG
-+
-AAAABFFFFFFFGGGGGGGC
-@M01368:8:000000000-A3GHV:1:1104:24927:22253/1
-AGCTAAAGTGAACTGTATCC
-+
-AAA?AFFF3DFFFGGBGFF3
-@M01368:8:000000000-A3GHV:1:1104:4705:7727/1
-CTACAAGCATTAATTAATTA
-+
-BBBBBFFBFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1104:7756:4776/1
-GGCCCATAACACTTGGGGGT
-+
-CCCCCCFFFFFFGGGGGGGE
-@M01368:8:000000000-A3GHV:1:1105:11310:21761/1
-ATAAATAATAGGATGAGGCA
-+
-CCCCBFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1105:11578:14676/1
-ATTGAACGTAGGTGCGATAA
-+
-CDDDDFFFBFFCGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1105:13575:19255/1
-TTAGTAAGTATGTTCGCCTG
-+
-AA1A1DFFFFFBGG3A111F
-@M01368:8:000000000-A3GHV:1:1105:15502:11453/1
-GTCCTACAAGCATTAATTAA
-+
-A3AAAFFFFFCFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1105:16787:9771/1
-GTTATTATTATGTCCTACAA
-+
-AABCCFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1105:17564:15723/1
-TATGCTCGCCTGTAATATTG
-+
-EDCCDFFCEEDCGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1105:18108:12091/1
-TAATAGGATGAGGCAGGAAT
-+
-CCCCCFBFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1105:18331:16683/1
-TCTGGTTCCTACTTCAGGGT
-+
-BABA?DFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1105:20577:18421/1
-GGGGGTAGCTAAAGTGAACT
-+
-ABB@3>AAFFFFFEAGGGFG
-@M01368:8:000000000-A3GHV:1:1105:21099:2895/1
-TAGCTAAAGTGAACTGTATC
-+
-CBCBBFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1105:26469:19245/1
-TTATGATTATGTCCTACAAG
-+
-BAAAAFFFBFFFGGGGGGFF
-@M01368:8:000000000-A3GHV:1:1105:7169:17145/1
-AACCTAAATAGCCCACACGT
-+
-CCCCCFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1106:15481:16176/1
-CTTTAGTAAGTATGTTCGCC
-+
-ABBBBFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1106:17389:18671/1
-CCATAACACTTGGGGGTAGC
-+
-3>>A@FFFFFFFGGGFEECF
-@M01368:8:000000000-A3GHV:1:1106:22927:3863/1
-GTATCCGACATCTGGTTCCT
-+
-A>ABAFA?D@AFGGGGFGGG
-@M01368:8:000000000-A3GHV:1:1106:23625:4236/1
-TAGCTAAAGTGAACTGTATC
-+
-@?AAAFFBFBFFFGGFGFGG
-@M01368:8:000000000-A3GHV:1:1106:26031:14826/1
-GATCACAGGTCTATCACCCT
-+
-ABBAAFFF@FFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1106:27607:15990/1
-GACAGATACTGCGACATAGG
-+
-BBBBBFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1106:6455:17124/1
-GTATCCGACATCTGGTTCCT
-+
-BBBAAFABDBBFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1107:14451:26748/1
-GTATGTTCGCCTGTAATATT
-+
-BABBBFFDABBBGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1107:18714:24788/1
-GTATGTTCGCCTGTAATATT
-+
-BBBCCFFFDEDEGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1107:21355:8333/1
-ATATTGAACGTAGGTGCGAT
-+
-ABCBBFFFFFAFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1107:22864:15712/1
-AATAAGACATCACGATGGAT
-+
-BBBBBFBBFFFFGGGGCEGG
-@M01368:8:000000000-A3GHV:1:1107:24817:14233/1
-CTTCAGGGTCATAAAACCTA
-+
-3AABABFFBFFFGCFGGGGG
-@M01368:8:000000000-A3GHV:1:1107:24830:14440/1
-AACTGTATCCGACATCTGGT
-+
-ABBBA@FFFFBBGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1107:26478:23602/1
-GCTAAAGTGAACTGTATCCG
-+
-1AAAAF1B13BBBEGGGFGG
-@M01368:8:000000000-A3GHV:1:1108:11059:23181/1
-GTTATTATTATGTCCTACAA
-+
-CCCCCFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1108:12233:13498/1
-TAGGTGCGATAAATAATAGG
-+
-BCCBAFFBCCCCGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1108:14328:14751/1
-ATAATAGGATGAGGCAGGAA
-+
-BCCCBFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1108:16350:22621/1
-TTAGTAAGTATGTTCGCCTG
-+
-AAAACFFFFFFFGGGFGGGG
-@M01368:8:000000000-A3GHV:1:1108:21791:23272/1
-CTTTAGTAAGTATGTTCGCC
-+
-3ABBBFFFFF@FGGGGGGGC
-@M01368:8:000000000-A3GHV:1:1108:23314:26089/1
-ATTGAACGTAGGTGCGATAA
-+
-BBBBBFFF?FFAGFBFGGEG
-@M01368:8:000000000-A3GHV:1:1108:25084:18127/1
-TTATTATGTCCTACAAGCAT
-+
-CCBBCFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1108:7626:10048/1
-ATCAAAGACAGATACTGCGA
-+
-CCCCCFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1109:11270:12255/1
-AATCAAAGACAGATACTGCG
-+
-CCCCCFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1109:19224:11655/1
-AAATAGCCCACACGTTCCCC
-+
-BBB@A4FFFBBBGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1109:21978:9490/1
-GGGTCATAAAACCTAAATAG
-+
-?A3AA?DFFFFFGGGGGGGF
-@M01368:8:000000000-A3GHV:1:1109:22910:4079/1
-TAATAGGATGAGGCAGGAAT
-+
-CCCCCFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1109:24217:19077/1
-AAAGTGAACTGTATCCGACA
-+
->AAA3BDFFFFDGBFFEEGG
-@M01368:8:000000000-A3GHV:1:1109:25237:11722/1
-CCTGTAATATTGAACGTAGG
-+
-AABBBFFFFFBBF5BGGFGA
-@M01368:8:000000000-A3GHV:1:1109:27103:7153/1
-GTAATATTGAACGTAGGTGC
-+
-33>A?FFFDFBFGCG4EGFG
-@M01368:8:000000000-A3GHV:1:1109:8763:7859/1
-TAGTAAGTATGTTCGCCTGT
-+
-BABAADFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1110:12808:18367/1
-AATTAACACACTTTAGTAAG
-+
-BBBBBFFFFBBFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1110:14751:26540/1
-TAACACTTGGGGGTAGCTAA
-+
-AAA?1CFFFFADGEEGFGB1
-@M01368:8:000000000-A3GHV:1:1110:15519:10008/1
-CTGTAATATTGAACGTAGGT
-+
-BBBBBFFFFFFFGGGGGGCB
-@M01368:8:000000000-A3GHV:1:1110:16559:7507/1
-CATTCAATTGTTATTATTAT
-+
-ABBBAFFFFFFFFGGGGGGG
-@M01368:8:000000000-A3GHV:1:1110:16700:6843/1
-AAATAATAGGATGAGGCAGG
-+
-BBBBBFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1110:18014:7856/1
-ATAAATAATAGGATGAGGCA
-+
-AABBBFFFFFFFCGGGCGGG
-@M01368:8:000000000-A3GHV:1:1110:23303:11846/1
-ACTTGGGGGTAGCTAAAGTG
-+
-3ABB?FAB?DAAGGGFBGGB
-@M01368:8:000000000-A3GHV:1:1111:13195:14562/1
-ATCAAAGACAGATACTGCGA
-+
-ABAAAFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1111:14287:20817/1
-GCCAACACGTTCCCCTTAAA
-+
->>A3>AAAACFFFGFGFGGG
-@M01368:8:000000000-A3GHV:1:1111:23624:14516/1
-GTCCTACAAGCATTAATTAA
-+
-3>3AAFFFFFAFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1111:7437:24508/1
-ACACACTTTAGTAAGTATGT
-+
-CBCCDCFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1111:8665:25542/1
-TCCTACTTCAGGGTCATAAA
-+
-AA3AAFFFFFFFGGGFGFFG
-@M01368:8:000000000-A3GHV:1:1111:9655:13916/1
-GGCCCATAACACTTGGGGGT
-+
-1>>1AAFFFFFBGGFE1EEE
-@M01368:8:000000000-A3GHV:1:1112:10272:7828/1
-GTATCCGACATCTGGTTCCT
-+
-AAABABBBBBBFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1112:9832:16531/1
-CTGTAATATTGAACGTAGGT
-+
-AABABFFFFFFFF4GGGGEB
-@M01368:8:000000000-A3GHV:1:1112:9832:6701/1
-CGCCTGTAATATTGAACGTA
-+
-AABBBBFFFFFFGGGFGGGG
-@M01368:8:000000000-A3GHV:1:1113:13532:8618/1
-GGAATCAAAGACAGATACTG
-+
-AA?AAFFFFFFFGGGGGFBF
-@M01368:8:000000000-A3GHV:1:1113:21679:18011/1
-GATCACAGGTCTATCACCCT
-+
-AA>1>11F1D1DDFFFFGGG
-@M01368:8:000000000-A3GHV:1:1113:25528:14016/1
-CCTACAAGCATTAATTAATT
-+
->AAAAFFFBFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1113:5741:16959/1
-TAGTAAGTATGTTCGCCTGT
-+
-ABBBBFFFFFFFGGGFGGGG
-@M01368:8:000000000-A3GHV:1:1114:10130:11959/1
-CGTTCCCCTTAAATAAGACA
-+
->A33>FFFFFF@FFFGGGGF
-@M01368:8:000000000-A3GHV:1:1114:14540:5315/1
-CTTTAGTAAGTATGTTCGCC
-+
-AABCCFFFFFFFGGGGGGGG
-@M01368:8:000000000-A3GHV:1:1114:15066:16302/1
-TAAATAAGACATCACGATGG
-+
-BBBBAFFBDFBFBGGGGGFG
-@M01368:8:000000000-A3GHV:1:1114:16639:15258/1
-CCTAAATAGCCCACACGTTC
-+
-11>>ABFFFFFFGGCGC1BB
-@M01368:8:000000000-A3GHV:1:1114:2404:13066/1
-TCCTACAAGCATTAATTAAT
-+
-CCCCCFFFFCFFGGGGGGGG
diff --git a/tools/trim_galore/test-data/sanger_full_range_report_results1.txt b/tools/trim_galore/test-data/sanger_full_range_report_results1.txt
index 29ee8cf9a4..9ff7587285 100644
--- a/tools/trim_galore/test-data/sanger_full_range_report_results1.txt
+++ b/tools/trim_galore/test-data/sanger_full_range_report_results1.txt
@@ -1,57 +1,60 @@
-
SUMMARISING RUN PARAMETERS
-==========================
+=========================
Input filename: input_1.fastq
Trimming mode: single-end
-Trim Galore version: 0.6.10
-Cutadapt version: 5.0
-Python version: 3.12.10
-Number of cores used for trimming: 4
+Trim Galore version: 2.2.0 (Oxidized Edition)
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
-Unable to auto-detect most prominent adapter from the first specified file (count smallRNA: 0, count Nextera: 0, count Illumina: 0)
-Defaulting to Illumina universal adapter ( AGATCGGAAGAGC ). Specify -a SEQUENCE to avoid this behavior).
-Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; default (inconclusive auto-detection))
-Maximum trimming error rate: 0.1 (default)
+Adapter sequence: 'AGATCGGAAGAGC' (user-specified or auto-detected)
+Maximum trimming error rate: 0.1
Minimum required adapter overlap (stringency): 1 bp
-Minimum required sequence length before a sequence gets removed: 20 bp
+Minimum required sequence length single-end: 20 bp
-This is cutadapt 5.0 with Python 3.12.10
-Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC input_1.fastq
-Processing single-end reads on 4 cores ...
+Trim Galore 2.2.0 (Oxidized Edition) — adapter trimming built in
+This is cutadapt 4.0 (compatible; for MultiQC backwards compatibility)
+Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC input_1.fastq
+Processing reads on 1 core in single-end mode ...
=== Summary ===
-Total reads processed: 2
-Reads with adapters: 1 (50.0%)
-Reads written (passing filters): 2 (100.0%)
+Total reads processed: 2
+Reads with adapters: 1 (50.0%)
+Reads written (passing filters): 2 (100.0%)
-Total basepairs processed: 188 bp
-Quality-trimmed: 20 bp (10.6%)
-Total written (filtered): 167 bp (88.8%)
+Total basepairs processed: 188 bp
+Quality-trimmed: 20 bp (10.6%)
+Total written (filtered): 167 bp (88.8%)
=== Adapter 1 ===
-Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 1 times
+Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 1 times.
-Minimum overlap: 1
No. of allowed errors:
1-9 bp: 0; 10-13 bp: 1
-Bases preceding removed adapters:
- A: 0.0%
- C: 100.0%
- G: 0.0%
- T: 0.0%
- none/other: 0.0%
-
Overview of removed sequences
length count expect max.err error counts
1 1 0.5 0 1
+
RUN STATISTICS FOR INPUT FILE: input_1.fastq
=============================================
2 sequences processed in total
Sequences removed because they became shorter than the length cutoff of 20 bp: 0 (0.0%)
+Sequences removed because they were longer than the upper length cutoff: 0 (0.0%)
+Sequences removed because of too many N bases: 0 (0.0%)
+
+
+Overview of removed sequences
+length count expect max.err error counts
+1 1 0.5 0 1
+
+
+RUN STATISTICS FOR INPUT FILE: sanger_full_range_original_sanger.fastqsanger
+=============================================
+2 sequences processed in total
+Sequences removed because they became shorter than the length cutoff of 20 bp: 0 (0.0%)
+Sequences removed because they were longer than the upper length cutoff: 0 (0.0%)
+Sequences removed because of too many N bases: 0 (0.0%)
diff --git a/tools/trim_galore/test-data/sanger_full_range_report_results1gz.txt b/tools/trim_galore/test-data/sanger_full_range_report_results1gz.txt
index 52a80d3192..154c0488d4 100644
--- a/tools/trim_galore/test-data/sanger_full_range_report_results1gz.txt
+++ b/tools/trim_galore/test-data/sanger_full_range_report_results1gz.txt
@@ -1,58 +1,48 @@
-
SUMMARISING RUN PARAMETERS
-==========================
+=========================
Input filename: input_1.fastq.gz
Trimming mode: single-end
-Trim Galore version: 0.6.10
-Cutadapt version: 5.0
-Python version: 3.12.10
-Number of cores used for trimming: 4
+Trim Galore version: 2.2.0 (Oxidized Edition)
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
-Unable to auto-detect most prominent adapter from the first specified file (count Illumina: 0, count smallRNA: 0, count Nextera: 0)
-Defaulting to Illumina universal adapter ( AGATCGGAAGAGC ). Specify -a SEQUENCE to avoid this behavior).
-Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; default (inconclusive auto-detection))
-Maximum trimming error rate: 0.1 (default)
+Adapter sequence: 'AGATCGGAAGAGC' (user-specified or auto-detected)
+Maximum trimming error rate: 0.1
Minimum required adapter overlap (stringency): 1 bp
-Minimum required sequence length before a sequence gets removed: 20 bp
+Minimum required sequence length single-end: 20 bp
Output file will be GZIP compressed
-This is cutadapt 5.0 with Python 3.12.10
-Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC input_1.fastq.gz
-Processing single-end reads on 4 cores ...
+Trim Galore 2.2.0 (Oxidized Edition) — adapter trimming built in
+This is cutadapt 4.0 (compatible; for MultiQC backwards compatibility)
+Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC input_1.fastq.gz
+Processing reads on 1 core in single-end mode ...
=== Summary ===
-Total reads processed: 2
-Reads with adapters: 1 (50.0%)
-Reads written (passing filters): 2 (100.0%)
+Total reads processed: 2
+Reads with adapters: 1 (50.0%)
+Reads written (passing filters): 2 (100.0%)
-Total basepairs processed: 188 bp
-Quality-trimmed: 20 bp (10.6%)
-Total written (filtered): 167 bp (88.8%)
+Total basepairs processed: 188 bp
+Quality-trimmed: 20 bp (10.6%)
+Total written (filtered): 167 bp (88.8%)
=== Adapter 1 ===
-Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 1 times
+Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 1 times.
-Minimum overlap: 1
No. of allowed errors:
1-9 bp: 0; 10-13 bp: 1
-Bases preceding removed adapters:
- A: 0.0%
- C: 100.0%
- G: 0.0%
- T: 0.0%
- none/other: 0.0%
-
Overview of removed sequences
length count expect max.err error counts
1 1 0.5 0 1
+
RUN STATISTICS FOR INPUT FILE: input_1.fastq.gz
=============================================
2 sequences processed in total
Sequences removed because they became shorter than the length cutoff of 20 bp: 0 (0.0%)
+Sequences removed because they were longer than the upper length cutoff: 0 (0.0%)
+Sequences removed because of too many N bases: 0 (0.0%)
diff --git a/tools/trim_galore/test-data/sanger_full_range_results1.fastq.gz b/tools/trim_galore/test-data/sanger_full_range_results1.fastq.gz
index 2a7f9fdd63..08f7272355 100644
Binary files a/tools/trim_galore/test-data/sanger_full_range_results1.fastq.gz and b/tools/trim_galore/test-data/sanger_full_range_results1.fastq.gz differ
diff --git a/tools/trim_galore/test-data/sanger_full_range_results1.fastqsanger b/tools/trim_galore/test-data/sanger_full_range_results1.fastqsanger
deleted file mode 100644
index 1b5d317c77..0000000000
--- a/tools/trim_galore/test-data/sanger_full_range_results1.fastqsanger
+++ /dev/null
@@ -1,8 +0,0 @@
-@FAKE0001 Original version has PHRED scores from 0 to 93 inclusive (in that order)
-ACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTAC
-+
-!"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~
-@FAKE0002 Original version has PHRED scores from 93 to 0 inclusive (in that order)
-CATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGC
-+
-~}|{zyxwvutsrqponmlkjihgfedcba`_^]\[ZYXWVUTSRQPONMLKJIHGFEDCBA@?>=<;:9876
diff --git a/tools/trim_galore/test-data/sanger_full_range_results2.fastq.gz b/tools/trim_galore/test-data/sanger_full_range_results2.fastq.gz
index 1a99365ec3..08f7272355 100644
Binary files a/tools/trim_galore/test-data/sanger_full_range_results2.fastq.gz and b/tools/trim_galore/test-data/sanger_full_range_results2.fastq.gz differ
diff --git a/tools/trim_galore/test-data/sanger_full_range_results2.fastqsanger b/tools/trim_galore/test-data/sanger_full_range_results2.fastqsanger
deleted file mode 100644
index 1b5d317c77..0000000000
--- a/tools/trim_galore/test-data/sanger_full_range_results2.fastqsanger
+++ /dev/null
@@ -1,8 +0,0 @@
-@FAKE0001 Original version has PHRED scores from 0 to 93 inclusive (in that order)
-ACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTAC
-+
-!"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~
-@FAKE0002 Original version has PHRED scores from 93 to 0 inclusive (in that order)
-CATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGC
-+
-~}|{zyxwvutsrqponmlkjihgfedcba`_^]\[ZYXWVUTSRQPONMLKJIHGFEDCBA@?>=<;:9876
diff --git a/tools/trim_galore/test-data/sanger_full_range_results3.fastq.gz b/tools/trim_galore/test-data/sanger_full_range_results3.fastq.gz
index 3126775b6a..08f7272355 100644
Binary files a/tools/trim_galore/test-data/sanger_full_range_results3.fastq.gz and b/tools/trim_galore/test-data/sanger_full_range_results3.fastq.gz differ
diff --git a/tools/trim_galore/test-data/sanger_full_range_results3.fastqsanger b/tools/trim_galore/test-data/sanger_full_range_results3.fastqsanger
deleted file mode 100644
index 1b5d317c77..0000000000
--- a/tools/trim_galore/test-data/sanger_full_range_results3.fastqsanger
+++ /dev/null
@@ -1,8 +0,0 @@
-@FAKE0001 Original version has PHRED scores from 0 to 93 inclusive (in that order)
-ACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTAC
-+
-!"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~
-@FAKE0002 Original version has PHRED scores from 93 to 0 inclusive (in that order)
-CATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGC
-+
-~}|{zyxwvutsrqponmlkjihgfedcba`_^]\[ZYXWVUTSRQPONMLKJIHGFEDCBA@?>=<;:9876
diff --git a/tools/trim_galore/test-data/trimmed_polyA_reads_pair1.fastq.gz b/tools/trim_galore/test-data/trimmed_polyA_reads_pair1.fastq.gz
new file mode 100644
index 0000000000..17020342c8
Binary files /dev/null and b/tools/trim_galore/test-data/trimmed_polyA_reads_pair1.fastq.gz differ
diff --git a/tools/trim_galore/test-data/trimmed_polyA_reads_pair1.fastqsanger b/tools/trim_galore/test-data/trimmed_polyA_reads_pair1.fastqsanger
deleted file mode 100644
index 490febc003..0000000000
--- a/tools/trim_galore/test-data/trimmed_polyA_reads_pair1.fastqsanger
+++ /dev/null
@@ -1,396 +0,0 @@
-@M01368:8:000000000-A3GHV:1:1101:6911:8255/1
-ATCTGGTTCCTACTTCAGGGCCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATT
-+
-BCCCCFFFFFFFGGGGGGGGGGGHHHHGHGHHHHHHHHHGGGGGGHHHHGHHHHHHHHHHGHHHHHHGGHGGHHHGHHHHFHHGHHHHHHHHHGHEHEFFGHHEGGCEFGGFHHHBGHHGHHHHGHFHHHGHGHGHGGCDFDDACGGGGGGGAAFFFFFFFFFBAFFFFFB;FFFFFFADDFFFFFFFFFFEFFFFFFFFFFBFFFFFFFFFFFFFFEFFFFFFFFBFEFFFFEFE;DFFFDFBFF/9BFB
-@M01368:8:000000000-A3GHV:1:1101:14518:9998/1
-GTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGAT
-+
-AAAAAFFFFFFFGGGGGGGGGGHGGHHHHGHHHHHHHGCGHHHHHHHHHHHHHHHGGGGGHHHHHHHHHGHHGFHFE5BGEEHFGGGHHHHHHHHFBHHGGGGFHGHHFGHHHHGHHHHHHGEGGGGFHFHGEGHHGGCDGDGHGGGDGGHGGCGGGHGHHH/ACDG?.1FGCDCCGCA.CC@CDCHFHGFFGGGEBFGAB//CEFBFGG.:;D;;A0AFFFFFB..:@ABFF//;BFFFFFBF/9D:A//
-@M01368:8:000000000-A3GHV:1:1101:18422:19051/1
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG
-+
-CCCCCFDDDDDFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHFHHHHGGGGHHHHHHHHHGHHHHHHHHHHHHGGHGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHGCGGGHHHHHHHHHHHHHHHHHHHHHHGFDHGFHCFGGGGFGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF;FFFFFFFFFFFFFFFFFFFFFFFFFFFFEFBFFFFFFFFFF:FFF.
-@M01368:8:000000000-A3GHV:1:1101:25545:21098/1
-ATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATAAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGGCTTATTTAAGGGGAACGGGTGGGG
-+
-33AA?DFD5BDFGGGFEBDGEGHEGHGEGHCEGGHHCHGHHFFHHGFGAGE53FF2FAFFGDE5FFFE5GFBFGAEE1GHHHGHHHEHE3FGHF@GEGEGGHHGG3FAGFFDE?EEE3GFEGFGFGGCG?GHHHFHGGGC@DHFFHD/AA@AAAAD2ADDFFGGGGGFGGHA?EAEFBEAGHFABFGG5FDF5DB1EEGAFDFB53FF5FH@G5FFEHGHEFHFFHBE333GF43GCGGGGE@0?BFGGB0B?FHGFDGGHHHBFFDEGGHGFFFDFE@<1>@FFFGHHHHFHEFGDABFFGG/@DCE---;--..;.../9
-@M01368:8:000000000-A3GHV:1:1103:25519:6301/1
-AAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGCATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATTTCGT
-+
-3A3AAFFFFBFFGGG2CF?4FGHGHHCHGGHHBHGFFAGGHHFFHHCGHFEGCECEGHFFFHGHHGFGHFGDAFDGFFEEFEGGBECC/;>=EF?AEFFFF;9;=DAFFFFBBFFFBFFF//;BFB.:FBFFBB/BFFFF/BFFE?EFAFEF9
-@1:A:M01368:8:000000000-A3GHV:1:1104:11928:24197/1_PolyA:1
-ACGTAGGTGCGATAAATAATGGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGAT
-+
-CDDCCFCFFFBCGGGGGGGGGGHHHGHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHGGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHGHHHHGHHGHHHHGGHGEEGGGGFHHFHHHGHGEHHHHHHHHHHHHHHHHHGGGGGGFGGGGGGGGFFGGGFGFGAEFEFGFEAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF@D
-@M01368:8:000000000-A3GHV:1:1104:12288:20635/1
-CACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGCCGCAGCATCTGTCTTTGATTCCTGCCT
-+
-ABA?AFBAB2>AFGGGGFFGD5GGHGFFHGHEGGGGHHHHFFHH5FHGHHHFFHEFHHHHHHGGHHHHHGFHGGHGGGGFHGGGFBHHHFGEGHHHHGHGGGGECEHHFHHGHHFFHFGGGHHHEGHDGHHGHGHFHHHECGCBCHHGFHHFHD1GFHEFHEF?GDCFFEFE0A@;@>BD/9BFBBBBFDFFFF99D=?>DDBFF-AFFEBB9BBEFEFAFFF//-@---;;FBBFBFB///;99999BA9
-@M01368:8:000000000-A3GHV:1:1104:13832:4398/1
-CACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGACAAAAAAAAACACACAACAACCAACACCCAGTAGACTAGTGCGTC
-+
-AAABABCAFFFFGGGGGGGGGGHFHHHHHHHHHGFFHHGGHGGGGGHHHHHHHHHHFHHHHGHHHHHGGHGHGGHGGGGGGGHGFFGGGGDGGHHHHGGGGFGGGGGCFGGEGCDGGGGGGGGGGGGEGF?;BFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF/FFFFFFC.;BFEAFFFFF;@FFBFFFFEF?/FFFFE----:...:...9....9.9...:.9//9////;/9-.-
-@M01368:8:000000000-A3GHV:1:1104:23670:4161/1
-ATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCC
-+
-AAAABFFFFFFFGGGGGGGCGBBGGHHHHHHHHHGHHHHHHHHHHFGGHHHHHHGGFHHHHHHHGGGGGFHHHGHHHHHGHHHHGHHGGGHGGGGHHHHHGHHHHHHEGGGFHHHGFFDGEGHGHFHGHHGEGGGHHBGHGGHGHGGGGG/@FCDFDGFHGGGDFFHFGFFGGGGGHHHHGGGGGGGEGCDG?GGBFFFEE00BFFGABBBAFGGGEGEA.9FFF/./;BFBF./AAF/BFFBBF/F/BFB
-@M01368:8:000000000-A3GHV:1:1104:24927:22253/1
-AGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTAT
-+
-AAA?AFFF3DFFFGGBGFF3EAEEEHFHHFHHHGHHHHDGHHGAEFGHHHGFCHHGHHHBGHHHAEGGHGGFHFBFHHHHHHHHHHFHH1EEHEEHH1FFDEEFCGHBGHHFHEHHHHHH1FCGHHHHEEFGEEGFFCHHH11>GHHF/FG2DGGCGAGGHGGGG@@@BEFFF?@@@FFEFFFFFF?@@FB@BB@FFFE=;==---A9EFBBB/;E;A9B/B/FFFEF/B///;/;9/A/;/9BFFFBFBB
-@M01368:8:000000000-A3GHV:1:1104:4705:7727/1
-CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG
-+
-BBBBBFFBFFFFGGGGGGGGGGHHHGHHHGHHHHH6GHHHHFFGGGHHHHHHHHHHHHHHHGGFGGGEGGGHHHHHHHHHHGHE3FGGGGHHHHHFHHHHHHHHHHHHGGGGGHHFHFCGFHHGGGGGGGHHGGGGHHGGGGGGHHHHGGGADCHHHHGDGGGGGGGGC@DGFHEHHHHHHGHHHHGFGDEGFGFFGGGGCFGBEGFGGGFG;C.BFFFFFFFBFBFFFF/F?.AFFF/BFFEFFFFFEFF
-@1:A:M01368:8:000000000-A3GHV:1:1104:7756:4776/1_PolyA:1
-GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTG
-+
-CCCCCCFFFFFFGGGGGGGEEFHHHHGHGHHHHHHGGHHHGGGGGGHHGHHHHHGHHGHHHGGFHHHHHHHHGHHHFHHGHHGFGGGGHHHHHHHHGHGHFFHFHHHHHHGGHGFGFHHHGHG?FGHHHHHHHHHHHHHHGHGHHGHGGCDGFFHHHHGHHHHHGHHHGFHGHFHGHGHGH/@CABFFFFFFFACAFF?BBFFBFD-@-.D>FFAF.F.:9---;AADFFFFFBEFFF:9FFFFBBFFFF
-@M01368:8:000000000-A3GHV:1:1105:11310:21761/1
-ATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTT
-+
-CCCCBFFFFFFFGGGGGGGGGGGHHHHHHHHHHHHGHHHHHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHGHHHGHHHGHGHHGHHHHGGHGGG=FFGEHHHHHGHFGGHHGHHHHHFHHHHHHHGGHHHHHHHGHEHHGHHHHHGGGGF9AGE?DEGFFGGGGGGGGGGGGGGFFFF/9FFBFFFFFFFEFFFBFFFFFFFFFFFFEFFF0
-@M01368:8:000000000-A3GHV:1:1105:11578:14676/1
-ATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTC
-+
-CDDDDFFFBFFCGGGGGGGGGGHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHFGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHHHHHHHHHGHHHHGGGGGGHGGHGHHHHHHH;CHHHHHHHHHHHHHHHGHGGGGFGGGFGGGGGGGGGGGGFGGGEGGFFFFFFFFFFFFFFFFFFFFFFFFF/FFFFFFFFFFFFFFFF0
-@M01368:8:000000000-A3GHV:1:1105:13575:19255/1
-TTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGTTCCCGTGAGTGGTTAATAGGGTGATAGGCCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG
-+
-AA1A1DFFFFFBGG3A111FFAEGDDBGFDHF00F01BBA00BFGEHFBFFA1FABB0FAE?CFHF1BGFHFB0011F121E//>>1F10>B?EFHGCC/?/BFG/EEGFFEE//BCFDE0?E@@B/?11?//<@?/F<<AAFFFFFEAGGGFGGAA6FHDGGCGFHFFHFHFHHHHHGDDDFFFHGFFHGBEGEGHHHFFFHGEEAEGHHHHHFHHHFHGHFEDGGEGBEAGEFHHFFHHHHHHGGHHHGHHGBGHHFHHFGHHHHG?CG?AFHH1FHHHGHFHHHF?DGHFFHCGAGEFHGCBBBB?BFFF?B-B.9/BFFFFFB=-AD?..@FFFF@@-@@?AFD.FFFBBEEBD9/:FFFFFBB/:FF9BF/BF.BBFF/:
-@M01368:8:000000000-A3GHV:1:1105:21099:2895/1
-TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTT
-+
-CBCBBFFFFFFFGGGGGGGGGGGGGFHHHHFHGHHHHHHFHHHHFGFHHHHHHGHHHHHHGHHEHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHHHHHHHFHHHHGHGHHHGGHHHHHGGHFFGHGCGGGHGHHGHHHFGHHFHH/FGGGHFGFEGGCFCCD;B@DFFFFFFFFFFFFFFFFFFFFFFFFF;F.BEFADFFFF.AEFFFFFFE;D.;:B00099:09BFBFFBFF.0:000BFFBF0
-@M01368:8:000000000-A3GHV:1:1105:26469:19245/1
-TTATGATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCAAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATG
-+
-BAAAAFFFBFFFGGGGGGFFGGHHHHHHHHHHHHGGHHGHHHGGHHHHHHHHHHHDEGGHGHHHHHGEDHFHGFHGGHHGGHGGEHHHGHHGHHGHHHGGEGGGHGHGHHHHHHGHHHHGHGGGGGHFHGGGGHHGGGGCDGHHHGD>A@FFFFFFFGGGFEECFGGFFB5GBHH5DFH5GBEEAEFHFHHF3F3GHFHHHEF3GAEHBGFHFFEFFHHHHFFB31FGGEGFHGEHHHHHHHHHHHHHHHHHEEEEGHHHFFF3GF3FGF44BBGEHHHHGG2B/BGGHE;B=.9.;.BBFFF..;A;/B/;B//;;//BBF/
-@2:A:M01368:8:000000000-A3GHV:1:1106:22927:3863/1_PolyA:2
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGACTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT
-+
-A>ABAFA?D@AFGGGGFGGGFGFHHHFFH4BCBGHHHHGFFF3G5BF3322EEAFHHHHHHAB5GHFHEHFHHH3BEEHFGF5@13FDF3GDGFHHFGHHHGHHHGFGGHHFCG//?/EGHHHG3F4BFFGHHHEDGFHHB/B/?FBF<@-9?BDEFFF?B--?DFFFB9FA>B;A.BFDB?AB.--@;-.FFAEFFFF9AE;DDFFFFB/9BFFFFFFFB:BF/BB/BFF//FFF-=--.99=--.9/
-@1:A:M01368:8:000000000-A3GHV:1:1106:23625:4236/1_PolyA:1
-TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATT
-+
-@?AAAFFBFBFFFGGFGFGGGEFCGGHHHHFGHGHFHGHHHHGGFGGFGHHGHGHHHHGHGFFHHGGGGGHHHHHHHHHBFHHEFHHHHHHGF?FAGHGFHHHGDGFHHGGGHGHFHHHGHHHGHHFHHGFFGFHHHGHHHHFFHHHHH/FGHHHHGHGHGDFFFE?FFF/--;;9/:BFFFBA///9----;B.//////;...---9--
-@M01368:8:000000000-A3GHV:1:1106:6455:17124/1
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG
-+
-BBBAAFABDBBFGGGGGGGGGGHHHGHHGHHHHHHHHHGHHGHHGHHHGHGGGEGHHHHHHHHHHHHHGHHHGHHHGGHGGHHHHHHHHHHHHHHHHHHGHHHHHHHHGHHHHHGGCFEHHHGFHHHHGHHFFH?GGHHHHGHGHGHHCFGGGGGGGFFFFFFFFFFFFFFFFFFFFADFA?.FEFAFFFEFFFFFFFFFFFFDABFBBFFFFFFFFEFFBFFFFFFFFFFF/FFBFFFFFFADE>BFFF9
-@1:A:M01368:8:000000000-A3GHV:1:1107:14451:26748/1_PolyA:1
-GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTG
-+
-BABBBFFDABBBGGGGGGGGGGHGHHHHGAGHGGGGGHHHHHHHHGHHHHHGGGGGGFFHHHHHHHHHFHHHHHGGGGCHFHH3FEHHGCGGGDGHHHGFGGHHGGGGHHHHHHG/DGGGHHHHGGGGGCCHGG?CGG1GHHGHHHHBGHHHFHHGHHHHGGADAD00ECEGHEFHHFFAEFFFFFGGFBFBFFFGFFG.;B/99;BCFGGGGEFFFFFFEDA./:ADEB?FBFFFF;FFEBFBFFB./9
-@1:A:M01368:8:000000000-A3GHV:1:1107:18714:24788/1_PolyA:1
-GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTG
-+
-BBBCCFFFDEDEGGGGGGGGGGHHHHHHGGHHGGHGGHHHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHCFGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHGHHHHHHHHHHHGHHHHGGEGEGCCEGGHHHHHHG:CAFFGGGGGGBFEFGGGGHGAFFGGFGGGGGGGEFFFFFFFFFEF/EDDFFFFFFFFFBFFFFFBBFFBBF
-@M01368:8:000000000-A3GHV:1:1107:21355:8333/1
-ATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATG
-+
-ABCBBFFFFFAFGGGGGGGGGGHHHHHHHHFHHHHHGGGGGHHHHHHFHHHGHHHHHHHGGGGGHHHHFFGHGHGGGGGGGHGGEGGGHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHGHHHFHHHHHHHHGHFFHHHHGGHGEGHHGHGFHHFHGHCEFHEGHGHHHGHHHGGGGGGCFFFFBFGGFGGGGFBG?FDGGGEGFFFFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFF.AA/9
-@M01368:8:000000000-A3GHV:1:1107:22864:15712/1
-AATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG
-+
-BBBBBFBBFFFFGGGGCEGGGGHGHHDGFHHHHHHHHHHHHHCHHGHFGBGDFGE?GHHGHFBHHFHHHHHHHHHGHFGFGHGHHGGC@DCFGHHGGGGGGGHHHHHHGGGGGFGGGGFFGGGGFADGFEFFFFFFFFFFF9;FFFF/BFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFEAECDFFFFFFFFFBFFFFFFFFFFFFFFEFBFBFFF/FFFDF-;-9;E@>-9DFFF/FFBF;-.:;.9A//
-@M01368:8:000000000-A3GHV:1:1107:24817:14233/1
-CTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACAT
-+
-3AABABFFBFFFGCFGGGGGGGHHHFGCFGGGFFGHHCGHHHGHGHGHBFFHHHEDGGFGFGHHFFFHGGEGHFHHGHFHGFFHHHFGHHHFHACECEHHHBGFHGFHHHFHHF4GFHFHFHEHGGE/EG:A-CFGGFGADGGGGFGGGGDAAAADFA.-AFFECFDAAFFFFEFFFFBFF;D;BFFFB/;BFFFFFFFFFFFFFFBFFBF/;//;BFFFF?FFFFFFEDAFFFFFBF//:BFBACF--9/
-@M01368:8:000000000-A3GHV:1:1107:24830:14440/1
-AACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACC
-+
-ABBBA@FFFFBBGGGGGGGGGGHGHHGHHGHHHGGHHHHHGHGHHGDHHHHHHAGGGGFHHGHHHHHHGHHHHHHHHHHHEGAFGFHFHHHHGEGHHHHHHHHHHHHHHHHHGGHHHGEFEGGHHGHHHHHGGHGFHH/?GHHHHGFGHGFHCGGBBDAFFFEFFFFFFFFFFFFFF=CDDADDFFFFEF=BFFFEFFFBFFFFEEBF./:FFFFFFFFFFFFFFFFFFFEFFFFFFFFFFF/BFBEFFC-
-@M01368:8:000000000-A3GHV:1:1107:26478:23602/1
-GCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATCTGTCTCTTATACACCTCTC
-+
-1AAAAF1B13BBBEGGGFGGGGGGHHDB1BGFHHHFGFGGG0F00BGBGFGGF1GBGB1BGCGAEE/FGFHGHFHHHHHBFF1B0FGGF//F//FGFGFFF/1@B1DFHHHGGEFEDHFDFAFF0B//1FDD1GFFHHDF?1BD>GF/GCAC0C@?@-;99BBB->@--;-B//99B--9-BF=A--A-:A----9AA9AFBFBBB?E@BBFFFFFFFBFFFFFBFFFBBFFF//B/////-;9
-@M01368:8:000000000-A3GHV:1:1108:11059:23181/1
-GTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGAT
-+
-CCCCCFFFFFFFGGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHGHHGGGGHIIIHHHHHHHGHHHHGHHHGGHGGHHHHHHHHHHHHHHGGGGGHHGHHHHHHHHHHHHHHHGGGGGHHHHHFGHHHGGGGGGHHHGGGGGHGGGGHHHHHHGGGGGGHGGHEFGGGGGHGGG-ECHHHGGGGGGGGGFGFFGGGGFGGGCF?EFGG0;BFFFFB?9AAFFFFFFBF;BBFFBFFFFE?F/
-@M01368:8:000000000-A3GHV:1:1108:12233:13498/1
-TAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCCTGTCTCTTATACAC
-+
-BCCBAFFBCCCCGGGGGGGGGGHHHHGGGGGHHHHHHGHGHBGFHHHHHGGGGGHHGGBFGHHGGGGGGGHHHGGGGHHGGGFHHHHHHGGGGGGHGHHGGGGGGGGGGGGGGHHHHFHHHGHGHHHHHHGHGHHGGFGGGDFFGGHHHGHGH/FHHHFHGHGHHGHHHGHHBAAAA3BDFFFFDGBFFEEGGGGHHGHFHHCFHGHGHHHGF3BFGFHGFEFHFHGH3EGFC1EEGBFGBHFGGFFHHHGHFHGHHGHGGHCEHEBCFF3ED3@GH4FDEG12FFHFFHG/G3FGA/@EFEFCG33FFGBBGHFHH/?BFBGFGFAGC2C/@-;@-;BFFEFADF?DFFFFFF?;AFFA;AD;..FEFFA--;9;D.9BFFBE?;.;///://///99//BB/9;F./B//BBFB9////99/
-@M01368:8:000000000-A3GHV:1:1109:25237:11722/1
-CCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATATCGTATGCCGT
-+
-AABBBFFFFFBBF5BGGFGABGHGGAEFGFHHHHFHCHFHHBFGGGEHBGGHFFFFGFAEF5FHH?EGGF5AEG3?EBFHD?C/EFHFFEGEE?CFGGGHHFHB3EEFG?EEGHCFGGGGGGGECA?FFFDFBFGCG4EGFG??GACFGGHFHCFHCHBECDEFCEGHHHHHFFGGF3AFFHHFGEGCGHHGEHGFGHHEGG?EFHHH?EGGHHGGGGHHHHHHEGGGGGHFHHGFDDGGACFCC@?/FDDGHHHGHEFFHHH0FDHHFFEDCD.CFHHGDFGGGGGEFGC?EGHHHB0CGHHGCGHHHHBEHGHH.::ACA;FECFGGGGGFFFEFGGGGGGGFFBBBFGBFFFEFFFFBFFFFFFBBFBFFB9..AF?FFFFFFF9
-@M01368:8:000000000-A3GHV:1:1110:14751:26540/1
-TAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACTGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGTTGTGCACGCGATAGCATTGCGCGACGCTGGCGCCGGCGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTG
-+
-AAA?1CFFFFADGEEGFGB1112DGDGHGFGHHCG/ECEBGGHDFGHHFHGHEB1BCGFFE2EGHHGHFFFHHBFFHEFCGGHHFHHHHH111BFFHGHEHBEHGGECEFHGFHFGGDBBGHHBHHHH0GH22FGHGFFFH1BFCG0F@FGG11@GDHFG1D/1FGFHH/FCG.>0><-<@CCFFG.CC-@C?C9FFF0C---;------;9----9-9@-AAFF9B9---9-/;B9BF/9BB//B/;///
-@M01368:8:000000000-A3GHV:1:1110:15519:10008/1
-CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACC
-+
-BBBBBFFFFFFFGGGGGGCBGGE?GGGHHHHHGHHBEGGHHGGGGGHHHHHHHHHHHGGHHHHGFGGGGHGHGCFGHHHGGGCGGHGHGGGGGHFGGGHHHHHHGCEFGGHHHHGGGGGGGHGG/CEGHHHHGFHHHGFHHGFFHGHGHFGGEGFDDGGBCGHHGFHH/CHHHHGHGHF0CFGGGGGGGFFFFFBFFFFFFGFFGGF?DGEDEEFEFFFF?FFFFFFFFFFFFFFFBFFF.BFFFBFBBA;
-@M01368:8:000000000-A3GHV:1:1110:16559:7507/1
-CATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAACTGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATC
-+
-ABBBAFFFFFFFFGGGGGGGFGGGGAGHHGCHHGHHHHHHHHHHHGHHGHHHHHHHHHHHHHHHHFGGGHHHHHHHHBDHHHHHGGHFEEGHGGHHHHHHHGHHHEHFGGGGGGHHHF33FGHHG3GDDGH//EEGHHFFBF?GHHFCC@DGFCGGB?DGHAFGGHHGFH0E??<@DGHGGCFGGF?DGGGFFGGGGGGGG.C/0BBF?FBBEGG0FFF.AA9;B9/E////99;;:.//:FFFFBBF//9
-@M01368:8:000000000-A3GHV:1:1110:16700:6843/1
-AAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCTGTCTCTTATACACATCTCCGAGCC
-+
-BBBBBFFFFFFFGGGGGGGGGGHHHHGHHHHHHHHHHHHGGGGGHHHHHGGHGHGGGGGGHHHGFFGHHGGGGHHHHHHCGG@FGHFHFEGGGGGGHGGGGDGHHHHHHHHHHHHHHGHHGHGHHGGGGGCGHEHGHHHFHHHFF.DGHHHHHHGHHHHHHGFGHGCGHGHHHHHHHHGHHHCD?:A::FCDGEGFGFFBFGBFGGGF00CBFFFF/BFBFFFFAE.FBBFBFFFFB/BFFBFFF/ADCAD
-@M01368:8:000000000-A3GHV:1:1110:18014:7856/1
-ATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGCAAAAAAAAAATAG
-+
-AABBBFFFFFFFCGGGCGGGGGGHHHHHGFHHHHHHGHHHGGGGGFHHGHFGGHHHGGFGGGHHGGGGGHHGGGGHGGHHHGGGGGGHHGHGDGGGGGGGGB/A99FE//BBFFF.9/FF?DA=@9./9
-@M01368:8:000000000-A3GHV:1:1110:23303:11846/1
-ACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGTGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTC
-+
-3ABB?FAB?DAAGGGFBGGBB5AFFFGFHHGGGEGHHHFHHF3EGFHHHHEFHHGGHHHGH3EFHHHHHBDGHHE?ECHGHGHHHHHHFHHHGHHHHHHHGGHGGFHHHFHHGFFHHFEGFHHHHHHEHHFDAGHHHH/BDDFGGH1FDBGHHHHHHHCGHHHFH?0CGCF>CC=@--AFBFFB?=BFDFFF//FDB;-9A-AD-.A.A=?-9-:B/AEB/BFF.A;DDFFFBBFFFF/BBFFFFBFF9B;
-@1:A:M01368:8:000000000-A3GHV:1:1111:13195:14562/1_PolyA:1
-ATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACCTGTCTCTTATACACATCTCCGAGCCCACGAG
-+
-ABAAAFFFFFFFGGGGGGGGGGGHHGG4FDFGFGGCGGGHGHG?EGHEGFFDHFHHHHGGFE@EHHGEEEGFCCEFFGFG/EHHHHHBFBGGHFHFHEHGHEFGGGED/EFDEFFEHHBGHHCFFFDFGGHHGFFFHHHHGFG>0<0DGGFHBGHFBDFGHGFFGGAGHHGCGBHFFHHHFFFBFGGGGF/:FFFCB9F9FF0FEFE?FF0BED-D.//BF9//BBFF/BFFFEFFFFED-DCFF?.:BF
-@M01368:8:000000000-A3GHV:1:1111:14287:20817/1
-GCCAACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTCATCGCACCTACGTTCACTATTACAGGCGAACATACTTATTAAAGTGTGTTAATTAAT
-+
->>A3>AAAACFFFGFGFGGGGGHGHHHHHHHGGGGGHGHFFCGHHGBGHFHHHHAGHHHHEHHAGFFEHEAE?GHHHHEGFFFDGHGHBFFHFHHHGHGHGHBECG@@@EHGGEGCGGGGFFGGGG?BGGGGGG.-C.FEDCFFFEFFFAF/99BEFA.:9BFB/9FFFEFFFFFFEFFF/FBBFFFFFFFFFFFB//;.@@//;DFFFF//BBFFFBF;A;DF=>B//;9B//B/;/;9//9//;B/:B9
-@M01368:8:000000000-A3GHV:1:1111:23624:14516/1
-GTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGGGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAG
-+
-3>3AAFFFFFAFGGGGGGGGBGHHHHHHGGHGGHEHGHADFHHHHEGGGHBGHFFHHEHHGHFHGG5FBEEGEGHHHGHHHEFDGHHFFGFGGHHGHHHGHBHGHFHFHHGGGGFCFBGFGE?GFBECEGDGHEHGDGCGHGC/DF@GHGFFCGGGGFFFFDD?@C@GFEC?-ADHFFHBGHFBHFG0CC//.;B0/CA.;AA.;@.AB/9BB/A/.AFFFE/BFFF///9B/FBDE9/;99/B/BFFEF/
-@M01368:8:000000000-A3GHV:1:1111:7437:24508/1
-ACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTGTCTCTTATACACATCTCCGAGCCCACGAG
-+
-CBCCDCFFFFFFGGGGGGGGGGGGGGHHHHHHHHHHHHHHHHGGGFFEHGGHHHHHHHHHHHGHHGGGGGHHHHHHFHHHHGHHHHGHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHGHHGGGGGGGHGGGGGCGHHHHGHHGHHHHHHHEDHHGHGGGGGD;BEGGGFFGGGGC:CFGGGGGGGGGFGGGFFFGFFFFF;FFF;BFFFFFFFFFFFB:FFFFD=CAEEEF>-
-@M01368:8:000000000-A3GHV:1:1111:8665:25542/1
-TCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTGTCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCG
-+
-AA3AAFFFFFFFGGGFGFFGGGGHGH4AGGGFHHCGEE2FGHHHHHHHHHHGFHGF53FHEGGFAF5CFHHGGFGHFHHGGHHHCHHHHHHHFHHGH?EEEG1FFFEHHFHFGGHHGG?F4FBGHGHCHEF0//-9--;0CFGG?@BGDGFFFFEFBBBDA?FFBFFFF=B;-9.AA9EFFFF/;9BD.//;9/9/B:/:BFBFFBFFF/B:BFF//;/B/FFFBFF.A-BFFFBA/F//BFFFBF/;/A-
-@1:A:M01368:8:000000000-A3GHV:1:1111:9655:13916/1_PolyA:1
-GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGCGGTGTCCACGCAATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTG
-+
-1>>1AAFFFFFBGGFE1EEEEGGHGHHH2FGHHHFGFGHGCFGGGEFHGGEGGHHHHGHEFBFEGHHHGHHBEHHHHGHGFHHHGGGGHHEGGGGHHHGFHGHHHH1FHHG?G/AGF1GBFGEFFHF2FDD0FG--99@=---AAABA-BF/B?A@F-9BBFF//BB/9
-@M01368:8:000000000-A3GHV:1:1112:10272:7828/1
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT
-+
-AAABABBBBBBFGGGGGGGGGGHGHHGHGHHHHHHHHHGHHHGGHDGHHHGGGGHHHHHHHHHHHHHHHFHGHHHHGGHGGHHHFHHHHHCGHFFHHHHHHHHHHHHHGHGEFGAEEEEHHGHHGHGHHHHHHHGHHHHHHGHGHFFBC/BA?BGGGFFFFFFFFFFFFFFFC-@DDFFFFFFFEFAFFFFFFBFBFFFFFFFFFFFFFFFFBFF->@FFFFFFFFFFBFBFAD--.99;A?////:.9A.
-@M01368:8:000000000-A3GHV:1:1112:9832:16531/1
-CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGGGCTGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCTTGAAGTAGGAACC
-+
-AABABFFFFFFFF4GGGGEBGEFGHGGHGHGHHHGGHFBGFGGGGGHHEFHGEHFHFHEHHHHHGCGGGHFHHCFGHE@1EEEFFGB?EEEEHBGGGCHHGHHBEE@>EAHHFAEBDGGGCEFF?CEGFFHHHHHFHHHFHGHCGCAHHF<..C.D=0CD.GFHHHG.CAGHC:CHB0GGGFFB0:CFA.A-;9.;000CBBFFBFGA.9AA;A;EDDE.ABBFFFF9;BEFFF/BF//9/B//9:B//..
-@1:A:M01368:8:000000000-A3GHV:1:1112:9832:6701/1_PolyA:1
-CGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGG
-+
-AABBBBFFFFFFGGGFGGGGGGHHHGGHGGHHHHHHHHHHHGHHGGGGGHHHHHHHHHHHHHHHHHHGGCGDHHHHFGGHHHGGGGGFGGHGGGGHHGGGGHHHHHHGGGGGGHHGGGGDGGFGHGGFCDCHHHHGHHHHGHHFAGFGGEHFGEG.C1>11F1D1DDFFFFGGG1FH3GHGHGHGHFHEC?EC0B1FBGFB1GHHH21AF2HH2FEGEHAE10AAE/>/FFHE?/E/?E1@EFFH?E@/EGGG//B11B//<@@C0F/<0AAAAFFFBFFFGGGGGGGGGGHHFHGHHHHGHHHHHHHHHHHGGGGHHHHFHHHHFHGHHHHGAFFEFHEGHHHHHHHHGHEHHGGFGGGHHHHHHFHHHHHGGHHHHGGGGGHFHHFF?HHGGFECEFFGHFFGFHGECDGHGBGFHGDF@@?CGFHCEGGGFD.CCC?EGHBHHHFHHFBCFFGEB/CEGGGGDAA.90C9CEBFGGBBF/9.9FBFFFBBFF//99FFFFEABF//99FFEFFFBFF
-@1:A:M01368:8:000000000-A3GHV:1:1113:5741:16959/1_PolyA:1
-TAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG
-+
-ABBBBFFFFFFFGGGFGGGGGGHHHGHHGGHBGHGAGFFCAFGHGFFGHHGFHHHHHGGGGGHGHHHHHHHHE3BFFHHHGG0EDF@GHFFGGGHGGGGGGGGGGGGGHHGGEEFHGFHHDDG@DGGGHHGDGGGGGHGG?CF?HHGHHHGHGHHHFFHGGGHHHHGGCD.;0A33>FFFFFF@FFFGGGGFGGD4EGGGGGHHGFFGHFGGHHHFEFHECFHHHEHGAGBCCGCCEGGHGHEGFBFHFHHHHGGGHFHGHEGGGFEGEGG??@DBGHGGC-@FFGHFHFHB-??DA-DD@9:BEBG@B?E?A.ABBFBFA??F.FF/BFFB/B9B/9;BF9FF9FFFFFFFFFFFFFF?BB?;9EE;-;DDB9//;B-B@;ABFFEFFFF/-.9A.;//9/BF./B/..9.9:.../////
-@M01368:8:000000000-A3GHV:1:1114:14540:5315/1
-CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGGGGGCTATTTAGGTTTTT
-+
-AABCCFFFFFFFGGGGGGGGGGHHHHHHHFHHHHGHHGHHGGGHGGHHHHHHHGHHHHHHGGGGGHHFHHHFGHHGGFHHHHHGGGGGHHHGHGGHHHGGGGGGHGHGGGGHHGGGGHHHHHEGDDFGFFFHHGGGGGCDAFCFGFDHHHHGGHGHHHHHHBCGEHHHHGGHG.ACGEHGG0CBFFF:A;BB0;09CGF00CFFFE0AA?//CFFFFFFFFFFFFFFFBEF;A.-=A--:BBFB90;;FE.
-@1:A:M01368:8:000000000-A3GHV:1:1114:15066:16302/1_PolyA:1
-TAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAACGGTTGTTAATTAATTATTGCTTGTAGGAC
-+
-BBBBAFFBDFBFBGGGGGFGGGBGFFFHGFHHGFFFHGHHHGHHHHFFHHHGHGC?AEFFHEFBFFFGHHHHH1FDGFFHGHGHFEGCGC-<>ABFFFFFFGGCGC1BBF1GHHHBHHHHGFHGH1A100AA/GGEHGFBAA1FFD2FHHHHECG2F1BB/E0FC/A>EE/FGGFGEF1EGBBEHHCGGGHBGEHFHE0B?E--;C?CCGGG@A@GBFBBBB09?@??A-AB--ABE@=-=-9AE9A;FFFFFE=9-//;//;9FF/////;;///9B///;/B////9FFBB;--A@-;/9;9-:-/;;FFFE9BF?@;-9-99/B9F/://///99/;
-@M01368:8:000000000-A3GHV:1:1114:2404:13066/1
-TCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCTGTCTATTATACACATC
-+
-CCCCCFFFFCFFGGGGGGGGGGHHHHHGHHHHHHHHHFFHHHHHGGGGHHHHHHHHFHHHHHHFGGHHGGHGGHHHHHHGHHFHHHHGGGGGGHHHHHHGHHHHHHHHHHGGGGGGGHH?FGHHHGGGGGGHHGGFGGHHGGGGHHHHHFGGGGFGHGHHGGGGGGGHGGGEGGHHGHHHHHHHHHGFBFFDA0FGGGFFGG0:EFGGGGGGGG;AEBF0B0BFFBFFFFFFFFFFFFFFFFFFFFFEFF0
diff --git a/tools/trim_galore/test-data/trimmed_polyA_reads_pair2.fastq.gz b/tools/trim_galore/test-data/trimmed_polyA_reads_pair2.fastq.gz
new file mode 100644
index 0000000000..921d8fc5cf
Binary files /dev/null and b/tools/trim_galore/test-data/trimmed_polyA_reads_pair2.fastq.gz differ
diff --git a/tools/trim_galore/test-data/trimmed_polyA_reads_pair2.fastqsanger b/tools/trim_galore/test-data/trimmed_polyA_reads_pair2.fastqsanger
deleted file mode 100644
index 490febc003..0000000000
--- a/tools/trim_galore/test-data/trimmed_polyA_reads_pair2.fastqsanger
+++ /dev/null
@@ -1,396 +0,0 @@
-@M01368:8:000000000-A3GHV:1:1101:6911:8255/1
-ATCTGGTTCCTACTTCAGGGCCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATT
-+
-BCCCCFFFFFFFGGGGGGGGGGGHHHHGHGHHHHHHHHHGGGGGGHHHHGHHHHHHHHHHGHHHHHHGGHGGHHHGHHHHFHHGHHHHHHHHHGHEHEFFGHHEGGCEFGGFHHHBGHHGHHHHGHFHHHGHGHGHGGCDFDDACGGGGGGGAAFFFFFFFFFBAFFFFFB;FFFFFFADDFFFFFFFFFFEFFFFFFFFFFBFFFFFFFFFFFFFFEFFFFFFFFBFEFFFFEFE;DFFFDFBFF/9BFB
-@M01368:8:000000000-A3GHV:1:1101:14518:9998/1
-GTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGAT
-+
-AAAAAFFFFFFFGGGGGGGGGGHGGHHHHGHHHHHHHGCGHHHHHHHHHHHHHHHGGGGGHHHHHHHHHGHHGFHFE5BGEEHFGGGHHHHHHHHFBHHGGGGFHGHHFGHHHHGHHHHHHGEGGGGFHFHGEGHHGGCDGDGHGGGDGGHGGCGGGHGHHH/ACDG?.1FGCDCCGCA.CC@CDCHFHGFFGGGEBFGAB//CEFBFGG.:;D;;A0AFFFFFB..:@ABFF//;BFFFFFBF/9D:A//
-@M01368:8:000000000-A3GHV:1:1101:18422:19051/1
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG
-+
-CCCCCFDDDDDFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHFHHHHGGGGHHHHHHHHHGHHHHHHHHHHHHGGHGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHGCGGGHHHHHHHHHHHHHHHHHHHHHHGFDHGFHCFGGGGFGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF;FFFFFFFFFFFFFFFFFFFFFFFFFFFFEFBFFFFFFFFFF:FFF.
-@M01368:8:000000000-A3GHV:1:1101:25545:21098/1
-ATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATAAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGGCTTATTTAAGGGGAACGGGTGGGG
-+
-33AA?DFD5BDFGGGFEBDGEGHEGHGEGHCEGGHHCHGHHFFHHGFGAGE53FF2FAFFGDE5FFFE5GFBFGAEE1GHHHGHHHEHE3FGHF@GEGEGGHHGG3FAGFFDE?EEE3GFEGFGFGGCG?GHHHFHGGGC@DHFFHD/AA@AAAAD2ADDFFGGGGGFGGHA?EAEFBEAGHFABFGG5FDF5DB1EEGAFDFB53FF5FH@G5FFEHGHEFHFFHBE333GF43GCGGGGE@0?BFGGB0B?FHGFDGGHHHBFFDEGGHGFFFDFE@<1>@FFFGHHHHFHEFGDABFFGG/@DCE---;--..;.../9
-@M01368:8:000000000-A3GHV:1:1103:25519:6301/1
-AAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGCATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATTTCGT
-+
-3A3AAFFFFBFFGGG2CF?4FGHGHHCHGGHHBHGFFAGGHHFFHHCGHFEGCECEGHFFFHGHHGFGHFGDAFDGFFEEFEGGBECC/;>=EF?AEFFFF;9;=DAFFFFBBFFFBFFF//;BFB.:FBFFBB/BFFFF/BFFE?EFAFEF9
-@1:A:M01368:8:000000000-A3GHV:1:1104:11928:24197/1_PolyA:1
-ACGTAGGTGCGATAAATAATGGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGAT
-+
-CDDCCFCFFFBCGGGGGGGGGGHHHGHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHGGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHGHHHHGHHGHHHHGGHGEEGGGGFHHFHHHGHGEHHHHHHHHHHHHHHHHHGGGGGGFGGGGGGGGFFGGGFGFGAEFEFGFEAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF@D
-@M01368:8:000000000-A3GHV:1:1104:12288:20635/1
-CACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGCCGCAGCATCTGTCTTTGATTCCTGCCT
-+
-ABA?AFBAB2>AFGGGGFFGD5GGHGFFHGHEGGGGHHHHFFHH5FHGHHHFFHEFHHHHHHGGHHHHHGFHGGHGGGGFHGGGFBHHHFGEGHHHHGHGGGGECEHHFHHGHHFFHFGGGHHHEGHDGHHGHGHFHHHECGCBCHHGFHHFHD1GFHEFHEF?GDCFFEFE0A@;@>BD/9BFBBBBFDFFFF99D=?>DDBFF-AFFEBB9BBEFEFAFFF//-@---;;FBBFBFB///;99999BA9
-@M01368:8:000000000-A3GHV:1:1104:13832:4398/1
-CACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGACAAAAAAAAACACACAACAACCAACACCCAGTAGACTAGTGCGTC
-+
-AAABABCAFFFFGGGGGGGGGGHFHHHHHHHHHGFFHHGGHGGGGGHHHHHHHHHHFHHHHGHHHHHGGHGHGGHGGGGGGGHGFFGGGGDGGHHHHGGGGFGGGGGCFGGEGCDGGGGGGGGGGGGEGF?;BFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF/FFFFFFC.;BFEAFFFFF;@FFBFFFFEF?/FFFFE----:...:...9....9.9...:.9//9////;/9-.-
-@M01368:8:000000000-A3GHV:1:1104:23670:4161/1
-ATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCC
-+
-AAAABFFFFFFFGGGGGGGCGBBGGHHHHHHHHHGHHHHHHHHHHFGGHHHHHHGGFHHHHHHHGGGGGFHHHGHHHHHGHHHHGHHGGGHGGGGHHHHHGHHHHHHEGGGFHHHGFFDGEGHGHFHGHHGEGGGHHBGHGGHGHGGGGG/@FCDFDGFHGGGDFFHFGFFGGGGGHHHHGGGGGGGEGCDG?GGBFFFEE00BFFGABBBAFGGGEGEA.9FFF/./;BFBF./AAF/BFFBBF/F/BFB
-@M01368:8:000000000-A3GHV:1:1104:24927:22253/1
-AGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTAT
-+
-AAA?AFFF3DFFFGGBGFF3EAEEEHFHHFHHHGHHHHDGHHGAEFGHHHGFCHHGHHHBGHHHAEGGHGGFHFBFHHHHHHHHHHFHH1EEHEEHH1FFDEEFCGHBGHHFHEHHHHHH1FCGHHHHEEFGEEGFFCHHH11>GHHF/FG2DGGCGAGGHGGGG@@@BEFFF?@@@FFEFFFFFF?@@FB@BB@FFFE=;==---A9EFBBB/;E;A9B/B/FFFEF/B///;/;9/A/;/9BFFFBFBB
-@M01368:8:000000000-A3GHV:1:1104:4705:7727/1
-CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG
-+
-BBBBBFFBFFFFGGGGGGGGGGHHHGHHHGHHHHH6GHHHHFFGGGHHHHHHHHHHHHHHHGGFGGGEGGGHHHHHHHHHHGHE3FGGGGHHHHHFHHHHHHHHHHHHGGGGGHHFHFCGFHHGGGGGGGHHGGGGHHGGGGGGHHHHGGGADCHHHHGDGGGGGGGGC@DGFHEHHHHHHGHHHHGFGDEGFGFFGGGGCFGBEGFGGGFG;C.BFFFFFFFBFBFFFF/F?.AFFF/BFFEFFFFFEFF
-@1:A:M01368:8:000000000-A3GHV:1:1104:7756:4776/1_PolyA:1
-GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTG
-+
-CCCCCCFFFFFFGGGGGGGEEFHHHHGHGHHHHHHGGHHHGGGGGGHHGHHHHHGHHGHHHGGFHHHHHHHHGHHHFHHGHHGFGGGGHHHHHHHHGHGHFFHFHHHHHHGGHGFGFHHHGHG?FGHHHHHHHHHHHHHHGHGHHGHGGCDGFFHHHHGHHHHHGHHHGFHGHFHGHGHGH/@CABFFFFFFFACAFF?BBFFBFD-@-.D>FFAF.F.:9---;AADFFFFFBEFFF:9FFFFBBFFFF
-@M01368:8:000000000-A3GHV:1:1105:11310:21761/1
-ATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTT
-+
-CCCCBFFFFFFFGGGGGGGGGGGHHHHHHHHHHHHGHHHHHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHGHHHGHHHGHGHHGHHHHGGHGGG=FFGEHHHHHGHFGGHHGHHHHHFHHHHHHHGGHHHHHHHGHEHHGHHHHHGGGGF9AGE?DEGFFGGGGGGGGGGGGGGFFFF/9FFBFFFFFFFEFFFBFFFFFFFFFFFFEFFF0
-@M01368:8:000000000-A3GHV:1:1105:11578:14676/1
-ATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTC
-+
-CDDDDFFFBFFCGGGGGGGGGGHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHFGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHHHHHHHHHGHHHHGGGGGGHGGHGHHHHHHH;CHHHHHHHHHHHHHHHGHGGGGFGGGFGGGGGGGGGGGGFGGGEGGFFFFFFFFFFFFFFFFFFFFFFFFF/FFFFFFFFFFFFFFFF0
-@M01368:8:000000000-A3GHV:1:1105:13575:19255/1
-TTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGTTCCCGTGAGTGGTTAATAGGGTGATAGGCCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG
-+
-AA1A1DFFFFFBGG3A111FFAEGDDBGFDHF00F01BBA00BFGEHFBFFA1FABB0FAE?CFHF1BGFHFB0011F121E//>>1F10>B?EFHGCC/?/BFG/EEGFFEE//BCFDE0?E@@B/?11?//<@?/F<<AAFFFFFEAGGGFGGAA6FHDGGCGFHFFHFHFHHHHHGDDDFFFHGFFHGBEGEGHHHFFFHGEEAEGHHHHHFHHHFHGHFEDGGEGBEAGEFHHFFHHHHHHGGHHHGHHGBGHHFHHFGHHHHG?CG?AFHH1FHHHGHFHHHF?DGHFFHCGAGEFHGCBBBB?BFFF?B-B.9/BFFFFFB=-AD?..@FFFF@@-@@?AFD.FFFBBEEBD9/:FFFFFBB/:FF9BF/BF.BBFF/:
-@M01368:8:000000000-A3GHV:1:1105:21099:2895/1
-TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTT
-+
-CBCBBFFFFFFFGGGGGGGGGGGGGFHHHHFHGHHHHHHFHHHHFGFHHHHHHGHHHHHHGHHEHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHHHHHHHFHHHHGHGHHHGGHHHHHGGHFFGHGCGGGHGHHGHHHFGHHFHH/FGGGHFGFEGGCFCCD;B@DFFFFFFFFFFFFFFFFFFFFFFFFF;F.BEFADFFFF.AEFFFFFFE;D.;:B00099:09BFBFFBFF.0:000BFFBF0
-@M01368:8:000000000-A3GHV:1:1105:26469:19245/1
-TTATGATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCAAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATG
-+
-BAAAAFFFBFFFGGGGGGFFGGHHHHHHHHHHHHGGHHGHHHGGHHHHHHHHHHHDEGGHGHHHHHGEDHFHGFHGGHHGGHGGEHHHGHHGHHGHHHGGEGGGHGHGHHHHHHGHHHHGHGGGGGHFHGGGGHHGGGGCDGHHHGD>A@FFFFFFFGGGFEECFGGFFB5GBHH5DFH5GBEEAEFHFHHF3F3GHFHHHEF3GAEHBGFHFFEFFHHHHFFB31FGGEGFHGEHHHHHHHHHHHHHHHHHEEEEGHHHFFF3GF3FGF44BBGEHHHHGG2B/BGGHE;B=.9.;.BBFFF..;A;/B/;B//;;//BBF/
-@2:A:M01368:8:000000000-A3GHV:1:1106:22927:3863/1_PolyA:2
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGACTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT
-+
-A>ABAFA?D@AFGGGGFGGGFGFHHHFFH4BCBGHHHHGFFF3G5BF3322EEAFHHHHHHAB5GHFHEHFHHH3BEEHFGF5@13FDF3GDGFHHFGHHHGHHHGFGGHHFCG//?/EGHHHG3F4BFFGHHHEDGFHHB/B/?FBF<@-9?BDEFFF?B--?DFFFB9FA>B;A.BFDB?AB.--@;-.FFAEFFFF9AE;DDFFFFB/9BFFFFFFFB:BF/BB/BFF//FFF-=--.99=--.9/
-@1:A:M01368:8:000000000-A3GHV:1:1106:23625:4236/1_PolyA:1
-TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATT
-+
-@?AAAFFBFBFFFGGFGFGGGEFCGGHHHHFGHGHFHGHHHHGGFGGFGHHGHGHHHHGHGFFHHGGGGGHHHHHHHHHBFHHEFHHHHHHGF?FAGHGFHHHGDGFHHGGGHGHFHHHGHHHGHHFHHGFFGFHHHGHHHHFFHHHHH/FGHHHHGHGHGDFFFE?FFF/--;;9/:BFFFBA///9----;B.//////;...---9--
-@M01368:8:000000000-A3GHV:1:1106:6455:17124/1
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG
-+
-BBBAAFABDBBFGGGGGGGGGGHHHGHHGHHHHHHHHHGHHGHHGHHHGHGGGEGHHHHHHHHHHHHHGHHHGHHHGGHGGHHHHHHHHHHHHHHHHHHGHHHHHHHHGHHHHHGGCFEHHHGFHHHHGHHFFH?GGHHHHGHGHGHHCFGGGGGGGFFFFFFFFFFFFFFFFFFFFADFA?.FEFAFFFEFFFFFFFFFFFFDABFBBFFFFFFFFEFFBFFFFFFFFFFF/FFBFFFFFFADE>BFFF9
-@1:A:M01368:8:000000000-A3GHV:1:1107:14451:26748/1_PolyA:1
-GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTG
-+
-BABBBFFDABBBGGGGGGGGGGHGHHHHGAGHGGGGGHHHHHHHHGHHHHHGGGGGGFFHHHHHHHHHFHHHHHGGGGCHFHH3FEHHGCGGGDGHHHGFGGHHGGGGHHHHHHG/DGGGHHHHGGGGGCCHGG?CGG1GHHGHHHHBGHHHFHHGHHHHGGADAD00ECEGHEFHHFFAEFFFFFGGFBFBFFFGFFG.;B/99;BCFGGGGEFFFFFFEDA./:ADEB?FBFFFF;FFEBFBFFB./9
-@1:A:M01368:8:000000000-A3GHV:1:1107:18714:24788/1_PolyA:1
-GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTG
-+
-BBBCCFFFDEDEGGGGGGGGGGHHHHHHGGHHGGHGGHHHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHCFGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHGHHHHHHHHHHHGHHHHGGEGEGCCEGGHHHHHHG:CAFFGGGGGGBFEFGGGGHGAFFGGFGGGGGGGEFFFFFFFFFEF/EDDFFFFFFFFFBFFFFFBBFFBBF
-@M01368:8:000000000-A3GHV:1:1107:21355:8333/1
-ATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATG
-+
-ABCBBFFFFFAFGGGGGGGGGGHHHHHHHHFHHHHHGGGGGHHHHHHFHHHGHHHHHHHGGGGGHHHHFFGHGHGGGGGGGHGGEGGGHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHGHHHFHHHHHHHHGHFFHHHHGGHGEGHHGHGFHHFHGHCEFHEGHGHHHGHHHGGGGGGCFFFFBFGGFGGGGFBG?FDGGGEGFFFFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFF.AA/9
-@M01368:8:000000000-A3GHV:1:1107:22864:15712/1
-AATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG
-+
-BBBBBFBBFFFFGGGGCEGGGGHGHHDGFHHHHHHHHHHHHHCHHGHFGBGDFGE?GHHGHFBHHFHHHHHHHHHGHFGFGHGHHGGC@DCFGHHGGGGGGGHHHHHHGGGGGFGGGGFFGGGGFADGFEFFFFFFFFFFF9;FFFF/BFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFEAECDFFFFFFFFFBFFFFFFFFFFFFFFEFBFBFFF/FFFDF-;-9;E@>-9DFFF/FFBF;-.:;.9A//
-@M01368:8:000000000-A3GHV:1:1107:24817:14233/1
-CTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACAT
-+
-3AABABFFBFFFGCFGGGGGGGHHHFGCFGGGFFGHHCGHHHGHGHGHBFFHHHEDGGFGFGHHFFFHGGEGHFHHGHFHGFFHHHFGHHHFHACECEHHHBGFHGFHHHFHHF4GFHFHFHEHGGE/EG:A-CFGGFGADGGGGFGGGGDAAAADFA.-AFFECFDAAFFFFEFFFFBFF;D;BFFFB/;BFFFFFFFFFFFFFFBFFBF/;//;BFFFF?FFFFFFEDAFFFFFBF//:BFBACF--9/
-@M01368:8:000000000-A3GHV:1:1107:24830:14440/1
-AACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACC
-+
-ABBBA@FFFFBBGGGGGGGGGGHGHHGHHGHHHGGHHHHHGHGHHGDHHHHHHAGGGGFHHGHHHHHHGHHHHHHHHHHHEGAFGFHFHHHHGEGHHHHHHHHHHHHHHHHHGGHHHGEFEGGHHGHHHHHGGHGFHH/?GHHHHGFGHGFHCGGBBDAFFFEFFFFFFFFFFFFFF=CDDADDFFFFEF=BFFFEFFFBFFFFEEBF./:FFFFFFFFFFFFFFFFFFFEFFFFFFFFFFF/BFBEFFC-
-@M01368:8:000000000-A3GHV:1:1107:26478:23602/1
-GCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATCTGTCTCTTATACACCTCTC
-+
-1AAAAF1B13BBBEGGGFGGGGGGHHDB1BGFHHHFGFGGG0F00BGBGFGGF1GBGB1BGCGAEE/FGFHGHFHHHHHBFF1B0FGGF//F//FGFGFFF/1@B1DFHHHGGEFEDHFDFAFF0B//1FDD1GFFHHDF?1BD>GF/GCAC0C@?@-;99BBB->@--;-B//99B--9-BF=A--A-:A----9AA9AFBFBBB?E@BBFFFFFFFBFFFFFBFFFBBFFF//B/////-;9
-@M01368:8:000000000-A3GHV:1:1108:11059:23181/1
-GTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGAT
-+
-CCCCCFFFFFFFGGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHGHHGGGGHIIIHHHHHHHGHHHHGHHHGGHGGHHHHHHHHHHHHHHGGGGGHHGHHHHHHHHHHHHHHHGGGGGHHHHHFGHHHGGGGGGHHHGGGGGHGGGGHHHHHHGGGGGGHGGHEFGGGGGHGGG-ECHHHGGGGGGGGGFGFFGGGGFGGGCF?EFGG0;BFFFFB?9AAFFFFFFBF;BBFFBFFFFE?F/
-@M01368:8:000000000-A3GHV:1:1108:12233:13498/1
-TAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCCTGTCTCTTATACAC
-+
-BCCBAFFBCCCCGGGGGGGGGGHHHHGGGGGHHHHHHGHGHBGFHHHHHGGGGGHHGGBFGHHGGGGGGGHHHGGGGHHGGGFHHHHHHGGGGGGHGHHGGGGGGGGGGGGGGHHHHFHHHGHGHHHHHHGHGHHGGFGGGDFFGGHHHGHGH/FHHHFHGHGHHGHHHGHHBAAAA3BDFFFFDGBFFEEGGGGHHGHFHHCFHGHGHHHGF3BFGFHGFEFHFHGH3EGFC1EEGBFGBHFGGFFHHHGHFHGHHGHGGHCEHEBCFF3ED3@GH4FDEG12FFHFFHG/G3FGA/@EFEFCG33FFGBBGHFHH/?BFBGFGFAGC2C/@-;@-;BFFEFADF?DFFFFFF?;AFFA;AD;..FEFFA--;9;D.9BFFBE?;.;///://///99//BB/9;F./B//BBFB9////99/
-@M01368:8:000000000-A3GHV:1:1109:25237:11722/1
-CCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATATCGTATGCCGT
-+
-AABBBFFFFFBBF5BGGFGABGHGGAEFGFHHHHFHCHFHHBFGGGEHBGGHFFFFGFAEF5FHH?EGGF5AEG3?EBFHD?C/EFHFFEGEE?CFGGGHHFHB3EEFG?EEGHCFGGGGGGGECA?FFFDFBFGCG4EGFG??GACFGGHFHCFHCHBECDEFCEGHHHHHFFGGF3AFFHHFGEGCGHHGEHGFGHHEGG?EFHHH?EGGHHGGGGHHHHHHEGGGGGHFHHGFDDGGACFCC@?/FDDGHHHGHEFFHHH0FDHHFFEDCD.CFHHGDFGGGGGEFGC?EGHHHB0CGHHGCGHHHHBEHGHH.::ACA;FECFGGGGGFFFEFGGGGGGGFFBBBFGBFFFEFFFFBFFFFFFBBFBFFB9..AF?FFFFFFF9
-@M01368:8:000000000-A3GHV:1:1110:14751:26540/1
-TAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACTGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGTTGTGCACGCGATAGCATTGCGCGACGCTGGCGCCGGCGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTG
-+
-AAA?1CFFFFADGEEGFGB1112DGDGHGFGHHCG/ECEBGGHDFGHHFHGHEB1BCGFFE2EGHHGHFFFHHBFFHEFCGGHHFHHHHH111BFFHGHEHBEHGGECEFHGFHFGGDBBGHHBHHHH0GH22FGHGFFFH1BFCG0F@FGG11@GDHFG1D/1FGFHH/FCG.>0><-<@CCFFG.CC-@C?C9FFF0C---;------;9----9-9@-AAFF9B9---9-/;B9BF/9BB//B/;///
-@M01368:8:000000000-A3GHV:1:1110:15519:10008/1
-CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACC
-+
-BBBBBFFFFFFFGGGGGGCBGGE?GGGHHHHHGHHBEGGHHGGGGGHHHHHHHHHHHGGHHHHGFGGGGHGHGCFGHHHGGGCGGHGHGGGGGHFGGGHHHHHHGCEFGGHHHHGGGGGGGHGG/CEGHHHHGFHHHGFHHGFFHGHGHFGGEGFDDGGBCGHHGFHH/CHHHHGHGHF0CFGGGGGGGFFFFFBFFFFFFGFFGGF?DGEDEEFEFFFF?FFFFFFFFFFFFFFFBFFF.BFFFBFBBA;
-@M01368:8:000000000-A3GHV:1:1110:16559:7507/1
-CATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAACTGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATC
-+
-ABBBAFFFFFFFFGGGGGGGFGGGGAGHHGCHHGHHHHHHHHHHHGHHGHHHHHHHHHHHHHHHHFGGGHHHHHHHHBDHHHHHGGHFEEGHGGHHHHHHHGHHHEHFGGGGGGHHHF33FGHHG3GDDGH//EEGHHFFBF?GHHFCC@DGFCGGB?DGHAFGGHHGFH0E??<@DGHGGCFGGF?DGGGFFGGGGGGGG.C/0BBF?FBBEGG0FFF.AA9;B9/E////99;;:.//:FFFFBBF//9
-@M01368:8:000000000-A3GHV:1:1110:16700:6843/1
-AAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCTGTCTCTTATACACATCTCCGAGCC
-+
-BBBBBFFFFFFFGGGGGGGGGGHHHHGHHHHHHHHHHHHGGGGGHHHHHGGHGHGGGGGGHHHGFFGHHGGGGHHHHHHCGG@FGHFHFEGGGGGGHGGGGDGHHHHHHHHHHHHHHGHHGHGHHGGGGGCGHEHGHHHFHHHFF.DGHHHHHHGHHHHHHGFGHGCGHGHHHHHHHHGHHHCD?:A::FCDGEGFGFFBFGBFGGGF00CBFFFF/BFBFFFFAE.FBBFBFFFFB/BFFBFFF/ADCAD
-@M01368:8:000000000-A3GHV:1:1110:18014:7856/1
-ATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGCAAAAAAAAAATAG
-+
-AABBBFFFFFFFCGGGCGGGGGGHHHHHGFHHHHHHGHHHGGGGGFHHGHFGGHHHGGFGGGHHGGGGGHHGGGGHGGHHHGGGGGGHHGHGDGGGGGGGGB/A99FE//BBFFF.9/FF?DA=@9./9
-@M01368:8:000000000-A3GHV:1:1110:23303:11846/1
-ACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGTGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTC
-+
-3ABB?FAB?DAAGGGFBGGBB5AFFFGFHHGGGEGHHHFHHF3EGFHHHHEFHHGGHHHGH3EFHHHHHBDGHHE?ECHGHGHHHHHHFHHHGHHHHHHHGGHGGFHHHFHHGFFHHFEGFHHHHHHEHHFDAGHHHH/BDDFGGH1FDBGHHHHHHHCGHHHFH?0CGCF>CC=@--AFBFFB?=BFDFFF//FDB;-9A-AD-.A.A=?-9-:B/AEB/BFF.A;DDFFFBBFFFF/BBFFFFBFF9B;
-@1:A:M01368:8:000000000-A3GHV:1:1111:13195:14562/1_PolyA:1
-ATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACCTGTCTCTTATACACATCTCCGAGCCCACGAG
-+
-ABAAAFFFFFFFGGGGGGGGGGGHHGG4FDFGFGGCGGGHGHG?EGHEGFFDHFHHHHGGFE@EHHGEEEGFCCEFFGFG/EHHHHHBFBGGHFHFHEHGHEFGGGED/EFDEFFEHHBGHHCFFFDFGGHHGFFFHHHHGFG>0<0DGGFHBGHFBDFGHGFFGGAGHHGCGBHFFHHHFFFBFGGGGF/:FFFCB9F9FF0FEFE?FF0BED-D.//BF9//BBFF/BFFFEFFFFED-DCFF?.:BF
-@M01368:8:000000000-A3GHV:1:1111:14287:20817/1
-GCCAACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTCATCGCACCTACGTTCACTATTACAGGCGAACATACTTATTAAAGTGTGTTAATTAAT
-+
->>A3>AAAACFFFGFGFGGGGGHGHHHHHHHGGGGGHGHFFCGHHGBGHFHHHHAGHHHHEHHAGFFEHEAE?GHHHHEGFFFDGHGHBFFHFHHHGHGHGHBECG@@@EHGGEGCGGGGFFGGGG?BGGGGGG.-C.FEDCFFFEFFFAF/99BEFA.:9BFB/9FFFEFFFFFFEFFF/FBBFFFFFFFFFFFB//;.@@//;DFFFF//BBFFFBF;A;DF=>B//;9B//B/;/;9//9//;B/:B9
-@M01368:8:000000000-A3GHV:1:1111:23624:14516/1
-GTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGGGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAG
-+
-3>3AAFFFFFAFGGGGGGGGBGHHHHHHGGHGGHEHGHADFHHHHEGGGHBGHFFHHEHHGHFHGG5FBEEGEGHHHGHHHEFDGHHFFGFGGHHGHHHGHBHGHFHFHHGGGGFCFBGFGE?GFBECEGDGHEHGDGCGHGC/DF@GHGFFCGGGGFFFFDD?@C@GFEC?-ADHFFHBGHFBHFG0CC//.;B0/CA.;AA.;@.AB/9BB/A/.AFFFE/BFFF///9B/FBDE9/;99/B/BFFEF/
-@M01368:8:000000000-A3GHV:1:1111:7437:24508/1
-ACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTGTCTCTTATACACATCTCCGAGCCCACGAG
-+
-CBCCDCFFFFFFGGGGGGGGGGGGGGHHHHHHHHHHHHHHHHGGGFFEHGGHHHHHHHHHHHGHHGGGGGHHHHHHFHHHHGHHHHGHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHGHHGGGGGGGHGGGGGCGHHHHGHHGHHHHHHHEDHHGHGGGGGD;BEGGGFFGGGGC:CFGGGGGGGGGFGGGFFFGFFFFF;FFF;BFFFFFFFFFFFB:FFFFD=CAEEEF>-
-@M01368:8:000000000-A3GHV:1:1111:8665:25542/1
-TCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTGTCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCG
-+
-AA3AAFFFFFFFGGGFGFFGGGGHGH4AGGGFHHCGEE2FGHHHHHHHHHHGFHGF53FHEGGFAF5CFHHGGFGHFHHGGHHHCHHHHHHHFHHGH?EEEG1FFFEHHFHFGGHHGG?F4FBGHGHCHEF0//-9--;0CFGG?@BGDGFFFFEFBBBDA?FFBFFFF=B;-9.AA9EFFFF/;9BD.//;9/9/B:/:BFBFFBFFF/B:BFF//;/B/FFFBFF.A-BFFFBA/F//BFFFBF/;/A-
-@1:A:M01368:8:000000000-A3GHV:1:1111:9655:13916/1_PolyA:1
-GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGCGGTGTCCACGCAATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTG
-+
-1>>1AAFFFFFBGGFE1EEEEGGHGHHH2FGHHHFGFGHGCFGGGEFHGGEGGHHHHGHEFBFEGHHHGHHBEHHHHGHGFHHHGGGGHHEGGGGHHHGFHGHHHH1FHHG?G/AGF1GBFGEFFHF2FDD0FG--99@=---AAABA-BF/B?A@F-9BBFF//BB/9
-@M01368:8:000000000-A3GHV:1:1112:10272:7828/1
-GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT
-+
-AAABABBBBBBFGGGGGGGGGGHGHHGHGHHHHHHHHHGHHHGGHDGHHHGGGGHHHHHHHHHHHHHHHFHGHHHHGGHGGHHHFHHHHHCGHFFHHHHHHHHHHHHHGHGEFGAEEEEHHGHHGHGHHHHHHHGHHHHHHGHGHFFBC/BA?BGGGFFFFFFFFFFFFFFFC-@DDFFFFFFFEFAFFFFFFBFBFFFFFFFFFFFFFFFFBFF->@FFFFFFFFFFBFBFAD--.99;A?////:.9A.
-@M01368:8:000000000-A3GHV:1:1112:9832:16531/1
-CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGGGCTGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCTTGAAGTAGGAACC
-+
-AABABFFFFFFFF4GGGGEBGEFGHGGHGHGHHHGGHFBGFGGGGGHHEFHGEHFHFHEHHHHHGCGGGHFHHCFGHE@1EEEFFGB?EEEEHBGGGCHHGHHBEE@>EAHHFAEBDGGGCEFF?CEGFFHHHHHFHHHFHGHCGCAHHF<..C.D=0CD.GFHHHG.CAGHC:CHB0GGGFFB0:CFA.A-;9.;000CBBFFBFGA.9AA;A;EDDE.ABBFFFF9;BEFFF/BF//9/B//9:B//..
-@1:A:M01368:8:000000000-A3GHV:1:1112:9832:6701/1_PolyA:1
-CGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGG
-+
-AABBBBFFFFFFGGGFGGGGGGHHHGGHGGHHHHHHHHHHHGHHGGGGGHHHHHHHHHHHHHHHHHHGGCGDHHHHFGGHHHGGGGGFGGHGGGGHHGGGGHHHHHHGGGGGGHHGGGGDGGFGHGGFCDCHHHHGHHHHGHHFAGFGGEHFGEG.C1>11F1D1DDFFFFGGG1FH3GHGHGHGHFHEC?EC0B1FBGFB1GHHH21AF2HH2FEGEHAE10AAE/>/FFHE?/E/?E1@EFFH?E@/EGGG//B11B//<@@C0F/<0AAAAFFFBFFFGGGGGGGGGGHHFHGHHHHGHHHHHHHHHHHGGGGHHHHFHHHHFHGHHHHGAFFEFHEGHHHHHHHHGHEHHGGFGGGHHHHHHFHHHHHGGHHHHGGGGGHFHHFF?HHGGFECEFFGHFFGFHGECDGHGBGFHGDF@@?CGFHCEGGGFD.CCC?EGHBHHHFHHFBCFFGEB/CEGGGGDAA.90C9CEBFGGBBF/9.9FBFFFBBFF//99FFFFEABF//99FFEFFFBFF
-@1:A:M01368:8:000000000-A3GHV:1:1113:5741:16959/1_PolyA:1
-TAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG
-+
-ABBBBFFFFFFFGGGFGGGGGGHHHGHHGGHBGHGAGFFCAFGHGFFGHHGFHHHHHGGGGGHGHHHHHHHHE3BFFHHHGG0EDF@GHFFGGGHGGGGGGGGGGGGGHHGGEEFHGFHHDDG@DGGGHHGDGGGGGHGG?CF?HHGHHHGHGHHHFFHGGGHHHHGGCD.;0A33>FFFFFF@FFFGGGGFGGD4EGGGGGHHGFFGHFGGHHHFEFHECFHHHEHGAGBCCGCCEGGHGHEGFBFHFHHHHGGGHFHGHEGGGFEGEGG??@DBGHGGC-@FFGHFHFHB-??DA-DD@9:BEBG@B?E?A.ABBFBFA??F.FF/BFFB/B9B/9;BF9FF9FFFFFFFFFFFFFF?BB?;9EE;-;DDB9//;B-B@;ABFFEFFFF/-.9A.;//9/BF./B/..9.9:.../////
-@M01368:8:000000000-A3GHV:1:1114:14540:5315/1
-CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGGGGGCTATTTAGGTTTTT
-+
-AABCCFFFFFFFGGGGGGGGGGHHHHHHHFHHHHGHHGHHGGGHGGHHHHHHHGHHHHHHGGGGGHHFHHHFGHHGGFHHHHHGGGGGHHHGHGGHHHGGGGGGHGHGGGGHHGGGGHHHHHEGDDFGFFFHHGGGGGCDAFCFGFDHHHHGGHGHHHHHHBCGEHHHHGGHG.ACGEHGG0CBFFF:A;BB0;09CGF00CFFFE0AA?//CFFFFFFFFFFFFFFFBEF;A.-=A--:BBFB90;;FE.
-@1:A:M01368:8:000000000-A3GHV:1:1114:15066:16302/1_PolyA:1
-TAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAACGGTTGTTAATTAATTATTGCTTGTAGGAC
-+
-BBBBAFFBDFBFBGGGGGFGGGBGFFFHGFHHGFFFHGHHHGHHHHFFHHHGHGC?AEFFHEFBFFFGHHHHH1FDGFFHGHGHFEGCGC-<>ABFFFFFFGGCGC1BBF1GHHHBHHHHGFHGH1A100AA/GGEHGFBAA1FFD2FHHHHECG2F1BB/E0FC/A>EE/FGGFGEF1EGBBEHHCGGGHBGEHFHE0B?E--;C?CCGGG@A@GBFBBBB09?@??A-AB--ABE@=-=-9AE9A;FFFFFE=9-//;//;9FF/////;;///9B///;/B////9FFBB;--A@-;/9;9-:-/;;FFFE9BF?@;-9-99/B9F/://///99/;
-@M01368:8:000000000-A3GHV:1:1114:2404:13066/1
-TCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCTGTCTATTATACACATC
-+
-CCCCCFFFFCFFGGGGGGGGGGHHHHHGHHHHHHHHHFFHHHHHGGGGHHHHHHHHFHHHHHHFGGHHGGHGGHHHHHHGHHFHHHHGGGGGGHHHHHHGHHHHHHHHHHGGGGGGGHH?FGHHHGGGGGGHHGGFGGHHGGGGHHHHHFGGGGFGHGHHGGGGGGGHGGGEGGHHGHHHHHHHHHGFBFFDA0FGGGFFGG0:EFGGGGGGGG;AEBF0B0BFFBFFFFFFFFFFFFFFFFFFFFFEFF0
diff --git a/tools/trim_galore/trim_galore.xml b/tools/trim_galore/trim_galore.xml
index 5d7e6f43c8..7a628cb1ad 100644
--- a/tools/trim_galore/trim_galore.xml
+++ b/tools/trim_galore/trim_galore.xml
@@ -4,16 +4,14 @@
macros.xml
-
+
trim_galore --version
input_1_trimmed.fq.gz ; fi
&&
- if [ -f input_1_val_1.fq.gz ] ; then mv input_1_val_1.fq.gz input_1_val_1.fq ; fi
+ if [ -f input_1_val_1.fq ] ; then gzip -n -c input_1_val_1.fq > input_1_val_1.fq.gz ; fi
&&
- if [ -f input_2_val_2.fq.gz ] ; then mv input_2_val_2.fq.gz input_2_val_2.fq ; fi
+ if [ -f input_2_val_2.fq ] ; then gzip -n -c input_2_val_2.fq > input_2_val_2.fq.gz ; fi
&&
- if [ -f input_1_unpaired_1.fq.gz ] ; then mv input_1_unpaired_1.fq.gz input_1_unpaired_1.fq ; fi
+ if [ -f input_1_unpaired_1.fq ] ; then gzip -n -c input_1_unpaired_1.fq > input_1_unpaired_1.fq.gz ; fi
&&
- if [ -f input_2_unpaired_2.fq.gz ] ; then mv input_2_unpaired_2.fq.gz input_2_unpaired_2.fq ; fi
+ if [ -f input_2_unpaired_2.fq ] ; then gzip -n -c input_2_unpaired_2.fq > input_2_unpaired_2.fq.gz ; fi
&&
- if [ -f input_1.clock_UMI.R1.fq.gz ] ; then mv input_1.clock_UMI.R1.fq.gz input_1.clock_UMI.R1.fq ; fi
+ if [ -f input_1.clock_UMI.R1.fq ] ; then gzip -n -c input_1.clock_UMI.R1.fq > input_1.clock_UMI.R1.fq.gz ; fi
&&
- if [ -f input_2.clock_UMI.R2.fq.gz ] ; then mv input_2.clock_UMI.R2.fq.gz input_2.clock_UMI.R2.fq ; fi
-
+ if [ -f input_2.clock_UMI.R2.fq ] ; then gzip -n -c input_2.clock_UMI.R2.fq > input_2.clock_UMI.R2.fq.gz ; fi
+
## Rename hardtrimmed files
#if $trimming.settingsType == 'custom'
&&
- if [ -f input_1.${trimming.hardtrim5}bp_5prime.fq.gz ] ; then mv input_1.${trimming.hardtrim5}bp_5prime.fq.gz input_1_hardtrim.fq ; fi
+ if ls input_1.*bp_5prime.fq.gz >/dev/null 2>&1 ; then mv input_1.*bp_5prime.fq.gz input_1_hardtrim.fq.gz ; fi
&&
- if [ -f input_2.${trimming.hardtrim5}bp_5prime.fq.gz ] ; then mv input_2.${trimming.hardtrim5}bp_5prime.fq.gz input_2_hardtrim.fq ; fi
+ if ls input_2.*bp_5prime.fq.gz >/dev/null 2>&1 ; then mv input_2.*bp_5prime.fq.gz input_2_hardtrim.fq.gz ; fi
&&
- if [ -f input_1.${trimming.hardtrim3}bp_3prime.fq.gz ] ; then mv input_1.${trimming.hardtrim3}bp_3prime.fq.gz input_1_hardtrim.fq ; fi
+ if ls input_1.*bp_3prime.fq.gz >/dev/null 2>&1 ; then mv input_1.*bp_3prime.fq.gz input_1_hardtrim.fq.gz ; fi
&&
- if [ -f input_2.${trimming.hardtrim3}bp_3prime.fq.gz ] ; then mv input_2.${trimming.hardtrim3}bp_3prime.fq.gz input_2_hardtrim.fq ; fi
+ if ls input_2.*bp_3prime.fq.gz >/dev/null 2>&1 ; then mv input_2.*bp_3prime.fq.gz input_2_hardtrim.fq.gz ; fi
&&
- if [ -f input_1.${trimming.hardtrim5}bp_5prime.fq ] ; then mv input_1.${trimming.hardtrim5}bp_5prime.fq input_1_hardtrim.fq ; fi
+ if ls input_1.*bp_5prime.fq >/dev/null 2>&1 ; then gzip -n -c input_1.*bp_5prime.fq > input_1_hardtrim.fq.gz ; fi
&&
- if [ -f input_2.${trimming.hardtrim5}bp_5prime.fq ] ; then mv input_2.${trimming.hardtrim5}bp_5prime.fq input_2_hardtrim.fq ; fi
+ if ls input_2.*bp_5prime.fq >/dev/null 2>&1 ; then gzip -n -c input_2.*bp_5prime.fq > input_2_hardtrim.fq.gz ; fi
&&
- if [ -f input_1.${trimming.hardtrim3}bp_3prime.fq ] ; then mv input_1.${trimming.hardtrim3}bp_3prime.fq input_1_hardtrim.fq ; fi
+ if ls input_1.*bp_3prime.fq >/dev/null 2>&1 ; then gzip -n -c input_1.*bp_3prime.fq > input_1_hardtrim.fq.gz ; fi
&&
- if [ -f input_2.${trimming.hardtrim3}bp_3prime.fq ] ; then mv input_2.${trimming.hardtrim3}bp_3prime.fq input_2_hardtrim.fq ; fi
+ if ls input_2.*bp_3prime.fq >/dev/null 2>&1 ; then gzip -n -c input_2.*bp_3prime.fq > input_2_hardtrim.fq.gz ; fi
#end if
## Trim Galore! run is finished. Move the report files to the proper place
@@ -193,7 +248,6 @@
&&
cat ./*_trimming_report.txt > '$report_file'
#end if
- && ls -lah
]]>
@@ -204,9 +258,8 @@
-
+
-
Instructs Trim Galore! to remove N bp from the 3' end of read 1 after adapter/quality trimming has been performed.
This may remove some unwanted bias from the 3' end that is not directly related to adapter sequence or basecall quality.
@@ -214,406 +267,406 @@
-
-
-
+
+
+
-
-
+
+
-
-
-
+
+
-
+
-
-
-
-
-
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-
-
+
+
+
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+
+
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+
+
+
+
+
+
+
+
+
+
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-
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-
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+
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+
+
-
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-
+
-
-
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+
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+
-
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+
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+
+
+
-
-
-
-
+
+
+
+
+
+
+
+
+
+
+
+
+
+
-
-
+
singlePaired['sPaired'] == "single"
- trimming['hardtrim3'] == '' and trimming['hardtrim5'] == ''
+ trimming['settingsType'] == "default" or (trimming['hardtrim3'] == '' and trimming['hardtrim5'] == '')
-
-
+
singlePaired['sPaired'] == "single"
trimming['settingsType'] == "custom"
trimming['hardtrim3'] != '' or trimming['hardtrim5'] != ''
-
-
-
+
+
singlePaired['sPaired'] == "paired_collection"
- trimming['hardtrim3'] == '' and trimming['hardtrim5'] == ''
- trimming['clock'] == False
+ trimming['settingsType'] == "default" or (trimming['hardtrim3'] == '' and trimming['hardtrim5'] == '')
+ trimming['settingsType'] == "default" or trimming['clock'] == False
-
-
-
+
+
params['settingsType'] == "custom"
params['retain_unpaired']['retain_unpaired_select'] == "retain_unpaired_output"
singlePaired['sPaired'] == "paired_collection"
- trimming['hardtrim3'] == '' and trimming['hardtrim5'] == ''
+ trimming['settingsType'] == "default" or (trimming['hardtrim3'] == '' and trimming['hardtrim5'] == '')
-
-
-
+
+
singlePaired['sPaired'] == "paired_collection"
trimming['settingsType'] == "custom"
trimming['hardtrim3'] or trimming['hardtrim5']
-
-
-
+
+
singlePaired['sPaired'] == "paired_collection"
trimming['settingsType'] == "custom"
trimming['clock']
-
-
-
+
singlePaired['sPaired'] == "paired"
- trimming['hardtrim3'] == '' and trimming['hardtrim5'] == ''
- trimming['clock'] == False
+ trimming['settingsType'] == "default" or (trimming['hardtrim3'] == '' and trimming['hardtrim5'] == '')
+ trimming['settingsType'] == "default" or trimming['clock'] == False
-
-
+
singlePaired['sPaired'] == "paired"
- trimming['hardtrim3'] == '' and trimming['hardtrim5'] == ''
- trimming['clock'] == False
+ trimming['settingsType'] == "default" or (trimming['hardtrim3'] == '' and trimming['hardtrim5'] == '')
+ trimming['settingsType'] == "default" or trimming['clock'] == False
-
-
+
params['settingsType'] == "custom"
params['retain_unpaired']['retain_unpaired_select'] == "retain_unpaired_output"
singlePaired['sPaired'] == "paired"
+ trimming['settingsType'] == "default" or (trimming['hardtrim3'] == '' and trimming['hardtrim5'] == '')
-
-
+
params['settingsType'] == "custom"
params['retain_unpaired']['retain_unpaired_select'] == "retain_unpaired_output"
singlePaired['sPaired'] == "paired"
+ trimming['settingsType'] == "default" or (trimming['hardtrim3'] == '' and trimming['hardtrim5'] == '')
-
-
+
singlePaired['sPaired'] == "paired"
trimming['settingsType'] == 'custom'
trimming['hardtrim3'] or trimming['hardtrim5']
-
-
+
singlePaired['sPaired'] == "paired"
trimming['settingsType'] == 'custom'
trimming['hardtrim3'] or trimming['hardtrim5']
-
-
+
singlePaired['sPaired'] == "paired"
trimming['settingsType'] == 'custom'
trimming['clock']
-
-
+
singlePaired['sPaired'] == "paired"
trimming['settingsType'] == 'custom'
trimming['clock']
-
params['settingsType'] == "custom"
params['report'] == True
-
-
-
+
+
-
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+
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+