diff --git a/tools/trim_galore/macros.xml b/tools/trim_galore/macros.xml index 7ebf438d14..f8b8d2a632 100644 --- a/tools/trim_galore/macros.xml +++ b/tools/trim_galore/macros.xml @@ -1,5 +1,5 @@ - 0.6.10 + 2.2.0 0 @@ -15,15 +15,19 @@ - - + + + + + + ^[ACTGNactgn]*$ @@ -38,7 +42,7 @@ ^[ACTGNactgn]*$ - + Instructs Trim Galore! to remove N bp from the 3' end of read 1 after adapter/quality trimming has been performed. This may remove some unwanted bias from the 3' end that is not directly related to adapter sequence or basecall quality. @@ -60,7 +64,7 @@ publisher = {GitHub}, journal = {GitHub repository}, url = {https://https://github.com/FelixKrueger/TrimGalore.com/fenderglass/Flye}} - + diff --git a/tools/trim_galore/test-data/mec_reads_pair1.fastq.gz b/tools/trim_galore/test-data/mec_reads_pair1.fastq.gz new file mode 100644 index 0000000000..229151a5a2 Binary files /dev/null and b/tools/trim_galore/test-data/mec_reads_pair1.fastq.gz differ diff --git a/tools/trim_galore/test-data/mec_reads_pair1.fastqsanger b/tools/trim_galore/test-data/mec_reads_pair1.fastqsanger deleted file mode 100644 index d44f953ce3..0000000000 --- a/tools/trim_galore/test-data/mec_reads_pair1.fastqsanger +++ /dev/null @@ -1,396 +0,0 @@ -@M01368:8:000000000-A3GHV:1:1101:6911:8255/1:R1:ATCTGGTT:R2:TCGCCTTA:F1:CCTA:F2:CCGC -TTCAGGGCCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATT -+ -GGGGGGGGGGHHHHGHGHHHHHHHHHGGGGGGHHHHGHHHHHHHHHHGHHHHHHGGHGGHHHGHHHHFHHGHHHHHHHHHGHEHEFFGHHEGGCEFGGFHHHBGHHGHHHHGHFHHHGHGHGHGGCDFDDACGGGGGGGAAFFFFFFFFFBAFFFFFB;FFFFFFADDFFFFFFFFFFEFFFFFFFFFFBFFFFFFFFFFFFFFEFFFFFFFFBFEFFFFEFE;DFFFDFBFF/9BFB -@M01368:8:000000000-A3GHV:1:1101:14518:9998/1:R1:GTTATTAT:R2:CATCACGA:F1:TATG:F2:TGGA -CCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGAT -+ -GGGGGGGGGHGGHHHHGHHHHHHHGCGHHHHHHHHHHHHHHHGGGGGHHHHHHHHHGHHGFHFE5BGEEHFGGGHHHHHHHHFBHHGGGGFHGHHFGHHHHGHHHHHHGEGGGGFHFHGEGHHGGCDGDGHGGGDGGHGGCGGGHGHHH/ACDG?.1FGCDCCGCA.CC@CDCHFHGFFGGGEBFGAB//CEFBFGG.:;D;;A0AFFFFFB..:@ABFF//;BFFFFFBF/9D:A// -@M01368:8:000000000-A3GHV:1:1101:18422:19051/1:R1:GTATCCGA:R2:CTACAAGC:F1:CATC:F2:ATTA -GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG -+ -GGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHFHHHHGGGGHHHHHHHHHGHHHHHHHHHHHHGGHGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHGCGGGHHHHHHHHHHHHHHHHHHHHHHGFDHGFHCFGGGGFGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF;FFFFFFFFFFFFFFFFFFFFFFFFFFFFEFBFFFFFFFFFF:FFF. -@M01368:8:000000000-A3GHV:1:1101:25545:21098/1:R1:ATTAATTA:R2:GCCCATAA:F1:ACAC:F2:CACT -CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATAAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGGCTTATTTAAGGGGAACGGGTGGGG -+ -GGFEBDGEGHEGHGEGHCEGGHHCHGHHFFHHGFGAGE53FF2FAFFGDE5FFFE5GFBFGAEE1GHHHGHHHEHE3FGHF@GEGEGGHHGG3FAGFFDE?EEE3GFEGFGFGGCG?GHHHFHGGGC@DHFFHD/A@FFFGHHHHFHEFGDABFFGG/@DCE---;--..;.../9 -@M01368:8:000000000-A3GHV:1:1103:25519:6301/1:R1:AAATAAGA:R2:CTGTAATA:F1:CATC:F2:TTGA -CGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGCATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATTTCGT -+ -GG2CF?4FGHGHHCHGGHHBHGFFAGGHHFFHHCGHFEGCECEGHFFFHGHHGFGHFGDAFDGFFEEFEGGBECC/;>=EF?AEFFFF;9;=DAFFFFBBFFFBFFF//;BFB.:FBFFBB/BFFFF/BFFE?EFAFEF9 -@M01368:8:000000000-A3GHV:1:1104:11928:24197/1:R1:ACGTAGGT:R2:CCTCGCTC:F1:GCGA:F2:CGGG -AAATAATGGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATA -+ -GGGGGGGGGHHHGHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHGGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHGHHHHGHHGHHHHGGHGEEGGGGFHHFHHHGHGEHHHHHHHHHHHHHHHHHGGGGGGFGGGGGGGGFFGGGFGFGAEFEFGFEAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF@D9 -@M01368:8:000000000-A3GHV:1:1104:12288:20635/1:R1:CACTTGGG:R2:CCTACAAG:F1:GGTA:F2:CATT -CTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGCCGCAGCATCTGTCTTTGATTCCTGCCT -+ -GGGGFFGD5GGHGFFHGHEGGGGHHHHFFHH5FHGHHHFFHEFHHHHHHGGHHHHHGFHGGHGGGGFHGGGFBHHHFGEGHHHHGHGGGGECEHHFHHGHHFFHFGGGHHHEGHDGHHGHGHFHHHECGCBCHHGFHHFHD1GFHEFHEF?GDCFFEFE0A@;@>BD/9BFBBBBFDFFFF99D=?>DDBFF-AFFEBB9BBEFEFAFFF//-@---;;FBBFBFB///;99999BA9 -@M01368:8:000000000-A3GHV:1:1104:13832:4398/1:R1:CACGATGG:R2:GACAGATA:F1:ATCA:F2:CTGC -AGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGACAAAAAAAAACACACAACAACCAACACCCAGTAGACTAGTGCGTC -+ -GGGGGGGGGHFHHHHHHHHHGFFHHGGHGGGGGHHHHHHHHHHFHHHHGHHHHHGGHGHGGHGGGGGGGHGFFGGGGDGGHHHHGGGGFGGGGGCFGGEGCDGGGGGGGGGGGGEGF?;BFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF/FFFFFFC.;BFEAFFFFF;@FFBFFFFEF?/FFFFE----:...:...9....9.9...:.9//9////;/9-.- -@M01368:8:000000000-A3GHV:1:1104:23670:4161/1:R1:ATTCAATT:R2:CGACATCT:F1:GTTA:F2:GGTT -TATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCC -+ -GGGGGGCGBBGGHHHHHHHHHGHHHHHHHHHHFGGHHHHHHGGFHHHHHHHGGGGGFHHHGHHHHHGHHHHGHHGGGHGGGGHHHHHGHHHHHHEGGGFHHHGFFDGEGHGHFHGHHGEGGGHHBGHGGHGHGGGGG/@FCDFDGFHGGGDFFHFGFFGGGGGHHHHGGGGGGGEGCDG?GGBFFFEE00BFFGABBBAFGGGEGEA.9FFF/./;BFBF./AAF/BFFBBF/F/BFB -@M01368:8:000000000-A3GHV:1:1104:24927:22253/1:R1:AGCTAAAG:R2:GTAATATT:F1:TGAA:F2:GAAC -TGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTAT -+ -GGBGFF3EAEEEHFHHFHHHGHHHHDGHHGAEFGHHHGFCHHGHHHBGHHHAEGGHGGFHFBFHHHHHHHHHHFHH1EEHEEHH1FFDEEFCGHBGHHFHEHHHHHH1FCGHHHHEEFGEEGFFCHHH11>GHHF/FG2DGGCGAGGHGGGG@@@BEFFF?@@@FFEFFFFFF?@@FB@BB@FFFE=;==---A9EFBBB/;E;A9B/B/FFFEF/B///;/;9/A/;/9BFFFBFBB -@M01368:8:000000000-A3GHV:1:1104:4705:7727/1:R1:CTACAAGC:R2:TCCTACTT:F1:ATTA:F2:CAGG -TTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG -+ -GGGGGGGGGHHHGHHHGHHHHH6GHHHHFFGGGHHHHHHHHHHHHHHHGGFGGGEGGGHHHHHHHHHHGHE3FGGGGHHHHHFHHHHHHHHHHHHGGGGGHHFHFCGFHHGGGGGGGHHGGGGHHGGGGGGHHHHGGGADCHHHHGDGGGGGGGGC@DGFHEHHHHHHGHHHHGFGDEGFGFFGGGGCFGBEGFGGGFG;C.BFFFFFFFBFBFFFF/F?.AFFF/BFFEFFFFFEFF -@M01368:8:000000000-A3GHV:1:1104:7756:4776/1:R1:GGCCCATA:R2:CTACAAGC:F1:ACAC:F2:ATTA -TGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGA -+ -GGGGGGEEFHHHHGHGHHHHHHGGHHHGGGGGGHHGHHHHHGHHGHHHGGFHHHHHHHHGHHHFHHGHHGFGGGGHHHHHHHHGHGHFFHFHHHHHHGGHGFGFHHHGHG?FGHHHHHHHHHHHHHHGHGHHGHGGCDGFFHHHHGHHHHHGHHHGFHGHFHGHGHGH/@CABFFFFFFFACAFF?BBFFBFD-@-.D>FFAF.F.:9---;AADFFFFFBEFFF:9FFFFBBFFFF/ -@M01368:8:000000000-A3GHV:1:1105:11310:21761/1:R1:ATAAATAA:R2:ACTTGGGG:F1:TAGG:F2:GTAG -TGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTT -+ -GGGGGGGGGGHHHHHHHHHHHHGHHHHHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHGHHHGHHHGHGHHGHHHHGGHGGG=FFGEHHHHHGHFGGHHGHHHHHFHHHHHHHGGHHHHHHHGHEHHGHHHHHGGGGF9AGE?DEGFFGGGGGGGGGGGGGGFFFF/9FFBFFFFFFFEFFFBFFFFFFFFFFFFEFFF0 -@M01368:8:000000000-A3GHV:1:1105:11578:14676/1:R1:ATTGAACG:R2:GCTCCGGG:F1:TAGG:F2:CCCA -GCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTC -+ -GGGGGGGGGHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHFGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHHHHHHHHHGHHHHGGGGGGHGGHGHHHHHHH;CHHHHHHHHHHHHHHHGHGGGGFGGGFGGGGGGGGGGGGFGGGEGGFFFFFFFFFFFFFFFFFFFFFFFFF/FFFFFFFFFFFFFFFF0 -@M01368:8:000000000-A3GHV:1:1105:13575:19255/1:R1:TTAGTAAG:R2:GGTTCCTA:F1:TATG:F2:CTTC -TCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGTTCCCGTGAGTGGTTAATAGGGTGATAGGCCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG -+ -G3A111FFAEGDDBGFDHF00F01BBA00BFGEHFBFFA1FABB0FAE?CFHF1BGFHFB0011F121E//>>1F10>B?EFHGCC/?/BFG/EEGFFEE//BCFDE0?E@@B/?11;B=.9.;.BBFFF..;A;/B/;B//;;//BBF/ -@M01368:8:000000000-A3GHV:1:1106:22927:3863/1:R1:GTATCCGA:R2:AAGACAGA:F1:CATC:F2:TACT -GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGACTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA -+ -GGGFGGGFGFHHHFFH4BCBGHHHHGFFF3G5BF3322EEAFHHHHHHAB5GHFHEHFHHH3BEEHFGF5@13FDF3GDGFHHFGHHHGHHHGFGGHHFCG//?/EGHHHG3F4BFFGHHHEDGFHHB/B/?FBF<@-9?BDEFFF?B--?DFFFB9FA>B;A.BFDB?AB.--@;-.FFAEFFFF9AE;DDFFFFB/9BFFFFFFFB:BF/BB/BFF//FFF-=--.99=--.9/// -@M01368:8:000000000-A3GHV:1:1106:23625:4236/1:R1:TAGCTAAA:R2:CACTTTAG:F1:GTGA:F2:TAAG -CTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTA -+ -GGFGFGGGEFCGGHHHHFGHGHFHGHHHHGGFGGFGHHGHGHHHHGHGFFHHGGGGGHHHHHHHHHBFHHEFHHHHHHGF?FAGHGFHHHGDGFHHGGGHGHFHHHGHHHGHHFHHGFFGFHHHGHHHHFFHHHHH/FGHHHHGHGHGDFFFE?FFF/--;;9/:BFFFBA///9----;B.//////;...---9--- -@M01368:8:000000000-A3GHV:1:1106:6455:17124/1:R1:GTATCCGA:R2:GCATTAAT:F1:CATC:F2:TAAT -GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG -+ -GGGGGGGGGHHHGHHGHHHHHHHHHGHHGHHGHHHGHGGGEGHHHHHHHHHHHHHGHHHGHHHGGHGGHHHHHHHHHHHHHHHHHHGHHHHHHHHGHHHHHGGCFEHHHGFHHHHGHHFFH?GGHHHHGHGHGHHCFGGGGGGGFFFFFFFFFFFFFFFFFFFFADFA?.FEFAFFFEFFFFFFFFFFFFDABFBBFFFFFFFFEFFBFFFFFFFFFFF/FFBFFFFFFADE>BFFF9 -@M01368:8:000000000-A3GHV:1:1107:14451:26748/1:R1:GTATGTTC:R2:AGCTAAAG:F1:GCCT:F2:TGAA -TAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGA -+ -GGGGGGGGGHGHHHHGAGHGGGGGHHHHHHHHGHHHHHGGGGGGFFHHHHHHHHHFHHHHHGGGGCHFHH3FEHHGCGGGDGHHHGFGGHHGGGGHHHHHHG/DGGGHHHHGGGGGCCHGG?CGG1GHHGHHHHBGHHHFHHGHHHHGGADAD00ECEGHEFHHFFAEFFFFFGGFBFBFFFGFFG.;B/99;BCFGGGGEFFFFFFEDA./:ADEB?FBFFFF;FFEBFBFFB./9. -@M01368:8:000000000-A3GHV:1:1107:18714:24788/1:R1:GTATGTTC:R2:GGCCCATA:F1:GCCT:F2:ACAC -TAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGA -+ -GGGGGGGGGHHHHHHGGHHGGHGGHHHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHCFGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHGHHHHHHHHHHHGHHHHGGEGEGCCEGGHHHHHHG:CAFFGGGGGGBFEFGGGGHGAFFGGFGGGGGGGEFFFFFFFFFEF/EDDFFFFFFFFFBFFFFFBBFFBBF/ -@M01368:8:000000000-A3GHV:1:1107:21355:8333/1:R1:ATATTGAA:R2:GTATCCGA:F1:CGTA:F2:CATC -GTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATG -+ -GGGGGGGGGHHHHHHHHFHHHHHGGGGGHHHHHHFHHHGHHHHHHHGGGGGHHHHFFGHGHGGGGGGGHGGEGGGHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHGHHHFHHHHHHHHGHFFHHHHGGHGEGHHGHGFHHFHGHCEFHEGHGHHHGHHHGGGGGGCFFFFBFGGFGGGGFBG?FDGGGEGFFFFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFF.AA/9 -@M01368:8:000000000-A3GHV:1:1107:22864:15712/1:R1:AATAAGAC:R2:GTAATATT:F1:ATCA:F2:GAAC -GATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG -+ -GGGCEGGGGHGHHDGFHHHHHHHHHHHHHCHHGHFGBGDFGE?GHHGHFBHHFHHHHHHHHHGHFGFGHGHHGGC@DCFGHHGGGGGGGHHHHHHGGGGGFGGGGFFGGGGFADGFEFFFFFFFFFFF9;FFFF/BFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFEAECDFFFFFFFFFBFFFFFFFFFFFFFFEFBFBFFF/FFFDF-;-9;E@>-9DFFF/FFBF;-.:;.9A// -@M01368:8:000000000-A3GHV:1:1107:24817:14233/1:R1:CTTCAGGG:R2:CTTTAGTA:F1:TCAT:F2:AGTA -AAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACAT -+ -CFGGGGGGGHHHFGCFGGGFFGHHCGHHHGHGHGHBFFHHHEDGGFGFGHHFFFHGGEGHFHHGHFHGFFHHHFGHHHFHACECEHHHBGFHGFHHHFHHF4GFHFHFHEHGGE/EG:A-CFGGFGADGGGGFGGGGDAAAADFA.-AFFECFDAAFFFFEFFFFBFF;D;BFFFB/;BFFFFFFFFFFFFFFBFFBF/;//;BFFFF?FFFFFFEDAFFFFFBF//:BFBACF--9/ -@M01368:8:000000000-A3GHV:1:1107:24830:14440/1:R1:AACTGTAT:R2:CTTTAGTA:F1:CCGA:F2:AGTA -ATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACC -+ -GGGGGGGGGHGHHGHHGHHHGGHHHHHGHGHHGDHHHHHHAGGGGFHHGHHHHHHGHHHHHHHHHHHEGAFGFHFHHHHGEGHHHHHHHHHHHHHHHHHGGHHHGEFEGGHHGHHHHHGGHGFHH/?GHHHHGFGHGFHCGGBBDAFFFEFFFFFFFFFFFFFF=CDDADDFFFFEF=BFFFEFFFBFFFFEEBF./:FFFFFFFFFFFFFFFFFFFEFFFFFFFFFFF/BFBEFFC- -@M01368:8:000000000-A3GHV:1:1107:26478:23602/1:R1:GCTAAAGT:R2:ATCAAAGA:F1:GAAC:F2:CAGA -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATCTGTCTCTTATACACCTCTC -+ -EGGGFGGGGGGHHDB1BGFHHHFGFGGG0F00BGBGFGGF1GBGB1BGCGAEE/FGFHGHFHHHHHBFF1B0FGGF//F//FGFGFFF/1@B1DFHHHGGEFEDHFDFAFF0B//1FDD1GFFHHDF?1BD>GF/GCAC0C@?@-;99BBB->@--;-B//99B--9-BF=A--A-:A----9AA9AFBFBBB?E@BBFFFFFFFBFFFFFBFFFBBFFF//B/////-;9 -@M01368:8:000000000-A3GHV:1:1108:11059:23181/1:R1:GTTATTAT:R2:AACTGTAT:F1:TATG:F2:CCGA -CCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGAT -+ -GGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHGHHGGGGHIIIHHHHHHHGHHHHGHHHGGHGGHHHHHHHHHHHHHHGGGGGHHGHHHHHHHHHHHHHHHGGGGGHHHHHFGHHHGGGGGGHHHGGGGGHGGGGHHHHHHGGGGGGHGGHEFGGGGGHGGG-ECHHHGGGGGGGGGFGFFGGGGFGGGCF?EFGG0;BFFFFB?9AAFFFFFFBF;BBFFBFFFFE?F/ -@M01368:8:000000000-A3GHV:1:1108:12233:13498/1:R1:TAGGTGCG:R2:GGTTCCTA:F1:ATAA:F2:CTTC -TAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCCTGTCTCTTATACAC -+ -GGGGGGGGGHHHHGGGGGHHHHHHGHGHBGFHHHHHGGGGGHHGGBFGHHGGGGGGGHHHGGGGHHGGGFHHHHHHGGGGGGHGHHGGGGGGGGGGGGGGHHHHFHHHGHGHHHHHHGHGHHGGFGGGDFFGGHHHGHGH/FHHHFHGHGHHGHHHGHHBACFHHGDFGGGGGEFGC?EGHHHB0CGHHGCGHHHHBEHGHH.::ACA;FECFGGGGGFFFEFGGGGGGGFFBBBFGBFFFEFFFFBFFFFFFBBFBFFB9..AF?FFFFFFF9 -@M01368:8:000000000-A3GHV:1:1110:14751:26540/1:R1:TAACACTT:R2:ATGTTCGC:F1:GGGG:F2:CTGT -TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACTGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGTTGTGCACGCGATAGCATTGCGCGACGCTGGCGCCGGCGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTG -+ -EEGFGB1112DGDGHGFGHHCG/ECEBGGHDFGHHFHGHEB1BCGFFE2EGHHGHFFFHHBFFHEFCGGHHFHHHHH111BFFHGHEHBEHGGECEFHGFHFGGDBBGHHBHHHH0GH22FGHGFFFH1BFCG0F@FGG11@GDHFG1D/1FGFHH/FCG.>0><-<@CCFFG.CC-@C?C9FFF0C---;------;9----9-9@-AAFF9B9---9-/;B9BF/9BB//B/;/// -@M01368:8:000000000-A3GHV:1:1110:15519:10008/1:R1:CTGTAATA:R2:GGTTCCTA:F1:TTGA:F2:CTTC -CGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACC -+ -GGGGGCBGGE?GGGHHHHHGHHBEGGHHGGGGGHHHHHHHHHHHGGHHHHGFGGGGHGHGCFGHHHGGGCGGHGHGGGGGHFGGGHHHHHHGCEFGGHHHHGGGGGGGHGG/CEGHHHHGFHHHGFHHGFFHGHGHFGGEGFDDGGBCGHHGFHH/CHHHHGHGHF0CFGGGGGGGFFFFFBFFFFFFGFFGGF?DGEDEEFEFFFF?FFFFFFFFFFFFFFFBFFF.BFFFBFBBA; -@M01368:8:000000000-A3GHV:1:1110:16559:7507/1:R1:CATTCAAT:R2:GCCCACAC:F1:TGTT:F2:GTTC -TTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAACTGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATC -+ -GGGGGGGFGGGGAGHHGCHHGHHHHHHHHHHHGHHGHHHHHHHHHHHHHHHHFGGGHHHHHHHHBDHHHHHGGHFEEGHGGHHHHHHHGHHHEHFGGGGGGHHHF33FGHHG3GDDGH//EEGHHFFBF?GHHFCC@DGFCGGB?DGHAFGGHHGFH0E??<@DGHGGCFGGF?DGGGFFGGGGGGGG.C/0BBF?FBBEGG0FFF.AA9;B9/E////99;;:.//:FFFFBBF//9 -@M01368:8:000000000-A3GHV:1:1110:16700:6843/1:R1:AAATAATA:R2:GTTCCTAC:F1:GGAT:F2:TTCA -AGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCTGTCTCTTATACACATCTCCGAGCC -+ -GGGGGGGGGHHHHGHHHHHHHHHHHHGGGGGHHHHHGGHGHGGGGGGHHHGFFGHHGGGGHHHHHHCGG@FGHFHFEGGGGGGHGGGGDGHHHHHHHHHHHHHHGHHGHGHHGGGGGCGHEHGHHHFHHHFF.DGHHHHHHGHHHHHHGFGHGCGHGHHHHHHHHGHHHCD?:A::FCDGEGFGFFBFGBFGGGF00CBFFFF/BFBFFFFAE.FBBFBFFFFB/BFFBFFF/ADCAD -@M01368:8:000000000-A3GHV:1:1110:18014:7856/1:R1:ATAAATAA:R2:CATCACGA:F1:TAGG:F2:TGGA -TGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGCAAAAAAAAAATAG -+ -GGGCGGGGGGHHHHHGFHHHHHHGHHHGGGGGFHHGHFGGHHHGGFGGGHHGGGGGHHGGGGHGGHHHGGGGGGHHGHGDGGGGGGGGB/A99FE//BBFFF.9/FF?DA=@9./9 -@M01368:8:000000000-A3GHV:1:1110:23303:11846/1:R1:ACTTGGGG:R2:GTCCTACA:F1:GTAG:F2:AGCA -TAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGTGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTC -+ -GGFBGGBB5AFFFGFHHGGGEGHHHFHHF3EGFHHHHEFHHGGHHHGH3EFHHHHHBDGHHE?ECHGHGHHHHHHFHHHGHHHHHHHGGHGGFHHHFHHGFFHHFEGFHHHHHHEHHFDAGHHHH/BDDFGGH1FDBGHHHHHHHCGHHHFH?0CGCF>CC=@--AFBFFB?=BFDFFF//FDB;-9A-AD-.A.A=?-9-:B/AEB/BFF.A;DDFFFBBFFFF/BBFFFFBFF9B; -@M01368:8:000000000-A3GHV:1:1111:13195:14562/1:R1:ATCAAAGA:R2:GTATCCGA:F1:CAGA:F2:CATC -ACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGA -+ -GGGGGGGGGGHHGG4FDFGFGGCGGGHGHG?EGHEGFFDHFHHHHGGFE@EHHGEEEGFCCEFFGFG/EHHHHHBFBGGHFHFHEHGHEFGGGED/EFDEFFEHHBGHHCFFFDFGGHHGFFFHHHHGFG>0<0DGGFHBGHFBDFGHGFFGGAGHHGCGBHFFHHHFFFBFGGGGF/:FFFCB9F9FF0FEFE?FF0BED-D.//BF9//BBFF/BFFFEFFFFED-DCFF?.:BFA -@M01368:8:000000000-A3GHV:1:1111:14287:20817/1:R1:GCCAACAC:R2:ATTCAATT:F1:GTTC:F2:GTTA -CCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTCATCGCACCTACGTTCACTATTACAGGCGAACATACTTATTAAAGTGTGTTAATTAAT -+ -GFGFGGGGGHGHHHHHHHGGGGGHGHFFCGHHGBGHFHHHHAGHHHHEHHAGFFEHEAE?GHHHHEGFFFDGHGHBFFHFHHHGHGHGHBECG@@@EHGGEGCGGGGFFGGGG?BGGGGGG.-C.FEDCFFFEFFFAF/99BEFA.:9BFB/9FFFEFFFFFFEFFF/FBBFFFFFFFFFFFB//;.@@//;DFFFF//BBFFFBF;A;DF=>B//;9B//B/;/;9//9//;B/:B9 -@M01368:8:000000000-A3GHV:1:1111:23624:14516/1:R1:GTCCTACA:R2:CGACATCT:F1:AGCA:F2:GGTT -TAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGGGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAG -+ -GGGGGGGBGHHHHHHGGHGGHEHGHADFHHHHEGGGHBGHFFHHEHHGHFHGG5FBEEGEGHHHGHHHEFDGHHFFGFGGHHGHHHGHBHGHFHFHHGGGGFCFBGFGE?GFBECEGDGHEHGDGCGHGC/DF@GHGFFCGGGGFFFFDD?@C@GFEC?-ADHFFHBGHFBHFG0CC//.;B0/CA.;AA.;@.AB/9BB/A/.AFFFE/BFFF///9B/FBDE9/;99/B/BFFEF/ -@M01368:8:000000000-A3GHV:1:1111:7437:24508/1:R1:ACACACTT:R2:ACATCACG:F1:TAGT:F2:ATGG -AGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTGTCTCTTATACACATCTCCGAGCCCACGAG -+ -GGGGGGGGGGGGGHHHHHHHHHHHHHHHHGGGFFEHGGHHHHHHHHHHHGHHGGGGGHHHHHHFHHHHGHHHHGHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHGHHGGGGGGGHGGGGGCGHHHHGHHGHHHHHHHEDHHGHGGGGGD;BEGGGFFGGGGC:CFGGGGGGGGGFGGGFFFGFFFFF;FFF;BFFFFFFFFFFFB:FFFFD=CAEEEF>- -@M01368:8:000000000-A3GHV:1:1111:8665:25542/1:R1:TCCTACTT:R2:CTTTAGTA:F1:CAGG:F2:AGTA -TCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTGTCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCG -+ -GGFGFFGGGGHGH4AGGGFHHCGEE2FGHHHHHHHHHHGFHGF53FHEGGFAF5CFHHGGFGHFHHGGHHHCHHHHHHHFHHGH?EEEG1FFFEHHFHFGGHHGG?F4FBGHGHCHEF0//-9--;0CFGG?@BGDGFFFFEFBBBDA?FFBFFFF=B;-9.AA9EFFFF/;9BD.//;9/9/B:/:BFBFFBFFF/B:BFF//;/B/FFFBFF.A-BFFFBA/F//BFFFBF/;/A- -@M01368:8:000000000-A3GHV:1:1111:9655:13916/1:R1:GGCCCATA:R2:TTAGTAAG:F1:ACAC:F2:TATG -TGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGCGGTGTCCACGCAATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGA -+ -GFE1EEEEGGHGHHH2FGHHHFGFGHGCFGGGEFHGGEGGHHHHGHEFBFEGHHHGHHBEHHHHGHGFHHHGGGGHHEGGGGHHHGFHGHHHH1FHHG?G/AGF1GBFGEFFHF2FDD0FG--99@=---AAABA-BF/B?A@F-9BBFF//BB/9/ -@M01368:8:000000000-A3GHV:1:1112:10272:7828/1:R1:GTATCCGA:R2:GTGCTCCG:F1:CATC:F2:GCTC -GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT -+ -GGGGGGGGGHGHHGHGHHHHHHHHHGHHHGGHDGHHHGGGGHHHHHHHHHHHHHHHFHGHHHHGGHGGHHHFHHHHHCGHFFHHHHHHHHHHHHHGHGEFGAEEEEHHGHHGHGHHHHHHHGHHHHHHGHGHFFBC/BA?BGGGFFFFFFFFFFFFFFFC-@DDFFFFFFFEFAFFFFFFBFBFFFFFFFFFFFFFFFFBFF->@FFFFFFFFFFBFBFAD--.99;A?////:.9A. -@M01368:8:000000000-A3GHV:1:1112:9832:16531/1:R1:CTGTAATA:R2:GTATCCGA:F1:TTGA:F2:CATC -CGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGGGCTGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCTTGAAGTAGGAACC -+ -4GGGGEBGEFGHGGHGHGHHHGGHFBGFGGGGGHHEFHGEHFHFHEHHHHHGCGGGHFHHCFGHE@1EEEFFGB?EEEEHBGGGCHHGHHBEE@>EAHHFAEBDGGGCEFF?CEGFFHHHHHFHHHFHGHCGCAHHF<..C.D=0CD.GFHHHG.CAGHC:CHB0GGGFFB0:CFA.A-;9.;000CBBFFBFGA.9AA;A;EDDE.ABBFFFF9;BEFFF/BF//9/B//9:B//.. -@M01368:8:000000000-A3GHV:1:1112:9832:6701/1:R1:CGCCTGTA:R2:GCTAAAGT:F1:ATAT:F2:GAAC -GAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGA -+ -GGFGGGGGGHHHGGHGGHHHHHHHHHHHGHHGGGGGHHHHHHHHHHHHHHHHHHGGCGDHHHHFGGHHHGGGGGFGGHGGGGHHGGGGHHHHHHGGGGGGHHGGGGDGGFGHGGFCDCHHHHGHHHHGHHFAGFGGEHFGEG.C/FFHE?/E/?E1@EFFH?E@/EGGG//B11B//<@@C0F/<0EE/FGGFGEF1EGBBEHHCGGGHBGEHFHE0B?E--;C?CCGGG@A@GBFBBBB09?@??A-AB--ABE@=-=-9AE9A;FFFFFE=9-//;//;9FF/////;;///9B///;/B////9FFBB;--A@-;/9;9-:-/;;FFFE9BF?@;-9-99/B9F/://///99/; -@M01368:8:000000000-A3GHV:1:1114:2404:13066/1:R1:TCCTACAA:R2:ATGGATCA:F1:GCAT:F2:CAGG -AATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCTGTCTATTATACACATC -+ -GGGGGGGGGHHHHHGHHHHHHHHHFFHHHHHGGGGHHHHHHHHFHHHHHHFGGHHGGHGGHHHHHHGHHFHHHHGGGGGGHHHHHHGHHHHHHHHHHGGGGGGGHH?FGHHHGGGGGGHHGGFGGHHGGGGHHHHHFGGGGFGHGHHGGGGGGGHGGGEGGHHGHHHHHHHHHGFBFFDA0FGGGFFGG0:EFGGGGGGGG;AEBF0B0BFFBFFFFFFFFFFFFFFFFFFFFFEFF0 diff --git a/tools/trim_galore/test-data/mec_reads_pair2.fastq.gz b/tools/trim_galore/test-data/mec_reads_pair2.fastq.gz new file mode 100644 index 0000000000..229151a5a2 Binary files /dev/null and b/tools/trim_galore/test-data/mec_reads_pair2.fastq.gz differ diff --git a/tools/trim_galore/test-data/mec_reads_pair2.fastqsanger b/tools/trim_galore/test-data/mec_reads_pair2.fastqsanger deleted file mode 100644 index 8aeb4eb0c7..0000000000 --- a/tools/trim_galore/test-data/mec_reads_pair2.fastqsanger +++ /dev/null @@ -1,396 +0,0 @@ -@M01368:8:000000000-A3GHV:1:1101:6911:8255/2:R1:ATCTGGTT:R2:TCGCCTTA:F1:CCTA:F2:CCGC -ACTCACCCACGGCGGCCATCAGCCGATACTAAGTTTGGGGTATGGTGGGGGGGATAATGAATTAGGTTGTGGGGGAGGGTTTGTGGTTGAGAGAAACACAAAAAACAATCTTATATATGGGTAGTCGTTTTGTATTGGTTTTTTGTTTTGTTTGTGTTTTGAGTGTCGGTTTAGTTCGGTGTACTAGGGGGGGTGGATGGGGTCGGCTGGTGAGGGGGTCTTAGTGTATTGAGTGTGG -+ -A111A100000000////011110//>>/12@1@22B/////1@>21/>>/-----9/;////9////--;-;-;-----;--------9/-/-///9-;-------9//////9/////-//-/9-;-;9--/////99-;--9-:-;----/---/-----////---9-/////--;A-//////---------9/-----;-----/-/-----;--;//////////9;///- -@M01368:8:000000000-A3GHV:1:1101:14518:9998/2:R1:GTTATTAT:R2:CATCACGA:F1:TATG:F2:TGGA -CACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATAA -+ -GGGGGGGGGHHHHHHHHHHHHHHHHGFHHHHGGGGGHGFHHHHHHHHHHHHHHHHHHHGHGGEHGGGGCGGGHHGGCGGGGGHHGHHHGGGGGGGG.BFFFGAGADFGAFDGFGGCFFF;DDFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFBFFFFFFFFFFFFFFFFFFF09FFFE00;BE@;DABBFFFFFBBFB00;F:9;FFBFFF9BFFFFFFFFFFFFF90/::BFFFBF0 -@M01368:8:000000000-A3GHV:1:1101:18422:19051/2:R1:GTATCCGA:R2:CTACAAGC:F1:CATC:F2:ATTA -TTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG -+ -GGGGGGGGGHHHGHHHHHHHHHHHHHHHHGGGGHHHHHHHHEHHHHHHHGGHHGGHGGHHHHHHHGHGGHHHGGGGGHGHHHHGGGHFHFHHHHHGGGGGHBFFCGDHHHGGGGGGHGGGGGGHHGCGGGFGHHBGGGGGFFFHEGGGGGCDCCE@EFGHHHHFHEGHGFFHHGB;ECBFGGGEFEFFGF0AFGFGFFG.;;DFFFFFFFFFF090BFFFE?FEFBBFBFFFB990BF -@M01368:8:000000000-A3GHV:1:1101:25545:21098/2:R1:ATTAATTA:R2:GCCCATAA:F1:ACAC:F2:CACT -GGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTTCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCAACCTATGTCGCAGTATCTGTCTTTGAT -+ -CGGCGGGCGHHHGHHHHCGHFGHFHGFGGCDGGBD5FGFFFHFHGGGHAEBFGHHHEHGGFHBGGFHHHGGGEEFHHHFHFFHDHGHGHHHHFFHHGGFAEGFFFFFBGHHHFFHHHHHHHFHFHHFHGFHGHGA/<@EFHEHH1GGHFFFHG1>=FGHFHGF.GE//-///F??//@BCBAC<<.<<///FDFACF?FHFGC//??1B0<FF>DFCFDFFFFFFFFF-BBAFFFFFFFFE@D9B;FBFFFFFFFFB9/9FFFEBF9/B/BB/BFAADDFFC-;;9-;@@9-.9/://///://///...- -@M01368:8:000000000-A3GHV:1:1103:25465:20010/2:R1:GACATAGG:R2:AACCTAAA:F1:GTGC:F2:TAGC -CACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGTGCACCCTATGTCCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGGTCTTGGTGGTCGTCCTCGTATTTGAACACATAATCGTG -+ -GGGGGGGGGHHHHHHGHHHGFHHHHHHHGGGGGHHHHHGHHHHHHHHEHHHHHGHGGHHGGGHHHHGGGGGHHHHHEHFGEHHHEE14BGHHHGHGHFCE/EFGGGGGGHGGGGGG?FFGGGG.ADGGGFFDA.FFEC--;9BBBD?FFFFFFFFBBFFFFFFFFFFFFFFFFFADFDFFC-BBB;BF-.;9:B9B9/9/;9;//;:.99/;-...:.../;//////.9/////.;. -@M01368:8:000000000-A3GHV:1:1103:25519:6301/2:R1:AAATAAGA:R2:CTGTAATA:F1:CATC:F2:TTGA -CGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATGCTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGGGATGTCTTATTTCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGAT -+ -FFGGGCAFGCGHGGGHGGGHHFHHHFHHGGGGGFFFHHHBHHHHG5BG5GFEEEE1FGFFGF1EGHGGG/BFHEF@EA1BE1@EED3?4DHGGGGEGHFFFDDG?DCDEG@<?@DGCAGC-;@DGFFHGCHEEFGGFGFB/;..BBB.CCE?A0FE0EBFF0FEF.;=DFFB//BFBF//;/:9BA.9FB9//;9//;9F;FF -@M01368:8:000000000-A3GHV:1:1104:13832:4398/2:R1:CACGATGG:R2:GACAGATA:F1:ATCA:F2:CTGC -ACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGGGGGTGCCGGCTCATTAAAAAAAGAAGATGTCGAGATGTCAGTGCGTGAGTGGAAGTG -+ -GGGGGGGGGGGGHGGGGGGGHHHGFGGHHFGGGHGHHGHGFGECGHHHHGGGGGGGHEGFGGGHHGHFHHHHFGHFHHHHGHHHHFGGFGG3FFGGHHHHHHH2?FFGHFHHHHHHHHHBGHDGC0FGHHGHHGHHHHHHHHHHFGHHGGGGGHG-:@?FAGA:.:09CB0000;9B0.9-;---:./----.0;B00;:0;;.../90009..-..00000000-...90:.00/00 -@M01368:8:000000000-A3GHV:1:1104:23670:4161/2:R1:ATTCAATT:R2:CGACATCT:F1:GTTA:F2:GGTT -CTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAA -+ -GGGGGGGGGHHFHHHHHHGHGHHHHHGCHHHHGGGGHHHHHHHHHHHHHHHGHHGHGHGGHGGHGHGGHHGFGHHGHHHEHHGHHHHHGHEHEHHHGGFGGHHHFHHHGHHHHHHHGHHHHG4EGGGFCBBCA?DGGGGGGCCAFFFFFFFFFFFFFFFFFDFFF.9@--=AFFFF;FBFFBEFFF9FBFFB9/FFFFFFB/FFFFFFFBFFFF/BBFBB/FFFE?.;@FFB?FBB// -@M01368:8:000000000-A3GHV:1:1104:24927:22253/2:R1:AGCTAAAG:R2:GTAATATT:F1:TGAA:F2:GAAC -TAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGGGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGGGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACGAG -+ -AFGG5GGGGHDGHHHHHHHHHHHHFHCFGC2F3BFGFGHBGHHFHEDHFGGGGGHHHG3F?GHHGGC/EFEHFGAEFFHGGAEHGGGG3EEGEEDHFHAAD@DGGGHG/AC1>GED-<<-.;C0:0C:C;GH:::::0B90;FFGEGFBFF0C.;9F0;0090;900;00FA9D.A...-9=BBFFFE/FE9;9E//BBBB.BBB///B/9/B..;. -@M01368:8:000000000-A3GHV:1:1104:4705:7727/2:R1:CTACAAGC:R2:TCCTACTT:F1:ATTA:F2:CAGG -TCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCG -+ -GFGGFGGGGGHHHHFHHHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHEDEFGHFHHHHGHGHHHHHHHGHHHHHHHHFHGHHHHHGGGGGHHHGHHHHHHHHHHHGHHHHFHGHGHGGHGGGDCD.;FFGGGGGGGGFFFFFFFFFFFFF.>FFFFFF-BBEFFFFFBFFFFF-DDFFFFFFEFFFFFFFFFFFF?F///9FFFFF/;FFFFF?AADCFFEFAFFEFFFFFB/;B/A9 -@M01368:8:000000000-A3GHV:1:1104:7756:4776/2:R1:GGCCCATA:R2:CTACAAGC:F1:ACAC:F2:ATTA -TTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG -+ -GGGGGGGGFFHHGHHGHHGGHGHHGFHHGGGGGHHHHHHHHGFF3GGGHGGGHFDHEGHHFHHHHGFHBCGHGEGFFFFFHHHHHGHHHHHGHHHGGGGGHHGHHGGHHHGGGGGGHHECCFDHHADGGHHHFGFDC////FHHHDGFCGCDEECF?EGGFFHHFEHFGGHHFFF/A900;C.9..C0990.;CFBFGE/9.9B99/;B.99/FB///9AEF/99/9/BBF/B/B//A -@M01368:8:000000000-A3GHV:1:1105:11310:21761/2:R1:ATAAATAA:R2:ACTTGGGG:F1:TAGG:F2:GTAG -TAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCTCC -+ -GGGGGGGGGHHHHHHHHGGGGGHHHHHHHHGHHHHHHHHHGHHHHHHHHGHHHHHHHHHHGGGGGHHHHHHHHHHGHHHHHHHHHHHGGHGGHHHHGHHHDGHHHHHHHHHHHHHHHHHGHHHHHGGGGGGHHHHHGHHHHHHHHFFHHHHHCCAHFHHGGFFFFFFFFFAA;BDDFFFF00.-=D.ABDA-.::AAF;F@.AAD./0000.9-;.9:00009:F090;FF000900; -@M01368:8:000000000-A3GHV:1:1105:11578:14676/2:R1:ATTGAACG:R2:GCTCCGGG:F1:TAGG:F2:CCCA -AACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGT -+ -GGGGGGGGGHGGGGGHHHHHHHGHHHHGHHHHHFFFGGHGHHHHHHHHHHHHHHHHFGHHHHHHHGHHGHFHFGHHHGGGGHHHHHHHHHHHHHHFHHHHHHHGGHGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHDGGCGHHHHHHHHHHHHHFFGGFGHHGHFFBGFGB2>F@;-A@FF<-;---@-;-----;-BFF/;---9//BBFFFF//B;FF///9BF///;/;B/BBFBFFBB/;-9--///;9/:-;BFFBBB9F -@M01368:8:000000000-A3GHV:1:1105:15502:11453/2:R1:GTCCTACA:R2:GATCACAG:F1:AGCA:F2:GTCT -TCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACCTGTCTCTTATACACATCTG -+ -GGGGGGGGGHHHHHHGHHHHHGGGGGFHFHHHHHHGHHHHHGHHHHHHGHFHGHGGGGGGGGHHHGGGGGGGGHHHGHGGGGGGGGGGGHHGGGFGGGFEGGGGFFFGGFGDGGGBFGFFFFFFFFFFFFFE0BFFFFFFFFFFFFFFFFFFF;BFFDFFFFBF0BFFBFFEEBFFAA009FFFFFBFFEBB0;.BFFBFFFFFFFFFFFF0;BFFFFE//090::B0000:009900 -@M01368:8:000000000-A3GHV:1:1105:16787:9771/2:R1:GTTATTAT:R2:TCCTACTT:F1:TATG:F2:CAGG -TCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCG -+ -GGGGGGGGGGHHHHHHHHHHHGGGGHHHHHHHHHHHHHHFHHHHHHHGGFGGHHEHHHHHAGHHGHBHHHGHHHHHHGHGHGHHHGGGGGHHHHHHHHHHHHHHHFFHHHHHGHGHGHHGGGACAFGGGGGG-AFGGFFFFFFFFFFFFFFFFFFFFF-9@AFEDFFFFFB.9-AABBBFFFEFFFFFFFFBFFFFFFEBB0BBBFFBFFBFBEDEAFFEB.ABFBFBFFFF0::/.- -@M01368:8:000000000-A3GHV:1:1105:17564:15723/2:R1:TATGCTCG:R2:ATCCGACA:F1:CCTG:F2:TCTG -TTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTT -+ -GGGGGGGGGHHHHGHHGHHHHHHGHHHHHHFHHHHGGGGHHGHFHHHHHHHFHHHHHHHGHGGHGGHGHHHHFHFHHGHHHHHHHHHHHHHEHGHHHHHGGGGGHHHHGEHHGHHHHHHGHFGHFHGGAHGGHGGGCCBDFGFFAFDBDDFFFFFBDA;AAD-ADC?AFEFB-@@AAEFEFFFFF..-:.B0:FFFFFFEF0BFF09FF.0;9BFFFFF0BFFFFFF?.::-909;.9 -@M01368:8:000000000-A3GHV:1:1105:18108:12091/2:R1:TAATAGGA:R2:GTATCCGA:F1:TGAG:F2:CATC -GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTACTGTCTCTTATACAC -+ -GGGGGGGGGHHGHHHHFHHHHHHFHGHHHHFHFHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHGHHHHGHFHGHHGHHHHHHGHHHHHHGHHHHHGGGGGHHHHHHHHGHHHHHGFGHHHHHGHDHGHHGCA;?AAFGFEFDFFF.FFFFEBDFFFFFFFFBFFFFFFFFFEFEFFFEFFFFFFF.:BFFF0BFFFFFFFFFFFF;0FF0;BFFFFFFFFBFFFBFEFFFF0 -@M01368:8:000000000-A3GHV:1:1105:18331:16683/2:R1:TCTGGTTC:R2:TTATTATG:F1:CTAC:F2:TCCT -CAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGGGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATATCT -+ -GGGGGGGGGHHHHHHHHHHHHHGHHHHHFHGHHGHHHHHGGFGHHIIHHIHHHHHHHFHCFGHEEHFFHGHHFGHGHGHFHHGGGGGGEEGHHEHFHHGGHFHHFGGGGDFGBCGG?DGHGGFCGGHHECEF?CHGGDGFFGGHHC?EEGGHHHH.>AEGGGHEE-AEG0C0GCCEFFFFGEFG/;CEGGGE.:CAE.;@.@EFBFFFF/..:BFF0:BB00FBFFFFBDAFFF0;0B -@M01368:8:000000000-A3GHV:1:1105:20577:18421/2:R1:GGGGGTAG:R2:ATGTCCTA:F1:CTAA:F2:CAAG -ATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGCCGAAAATACCAAATGCATGGAGAGCTCCCGTGAATGGGTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTT -+ -CFFGGGFGGHHHHHHHCEGHHCHFGHCAFEFHHHGGAEGGHHDHHHBGHBGHHH?5BGE1AEEFHHHHHGHFHBGHGEGGGGHBFHHFGHBGCFFG4EGHGG?E/FG3F3F/FGCDECEA/FGG/CEDBB//?BHEHHF0ADC@D>D9@DDCFFFFFFCFDFE.AA;/BB/.;-.;BFBBBFFBFFFFFFF/9FF?/;/BBFFFFF/;FFB/ -@M01368:8:000000000-A3GHV:1:1106:17389:18671/2:R1:CCATAACA:R2:GTATGTTC:F1:CTTG:F2:GCCT -TAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTCTTTAGGGTTTATGACCCTGA -+ -GGGGGGGGGHGHGHHGFGFGFHGGHHGHHHHHHHHFHHGGGGAGFBGHHHFBBGH5GDHH5EEEGEHEGFBF1DGFDGFGGGBBCEEGEHFEFDDHFBFHFD/@BGDGDHHGDCCFCD/C<->.ADDGE1GA;9.FFFFFFD-99ADFFD-.9FADF--9..;AAFEFFEAA-;.///BB:BBF/BBB//B//99/;BBB/9////F -@M01368:8:000000000-A3GHV:1:1107:18714:24788/2:R1:GTATGTTC:R2:GGCCCATA:F1:GCCT:F2:ACAC -TGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTAGGTCGCAGGAACTGTCCTTGT -+ -GGGGGGEGGHHHHGGFFHHHHHHHHHHGGGGGHHHHGHHHHHHHHHHHHHFHHHHHHHHGHHHHHHGHHHHGGCGHHHHHHHHHHHHHHHGHHHHHGDGHGGHHHHHHHHGHHHHHHHHHHHGHHHHHHEHHHGHFGGGGHHHHEHFHHGHFHHF.>GGHHHGHGHGGHCFFCDFDFFFFFFAAF.FFFFFEFAFCFFA..@.AFFFC9--9;.;A///00--;A-.;.000000090 -@M01368:8:000000000-A3GHV:1:1107:21355:8333/2:R1:ATATTGAA:R2:GTATCCGA:F1:CGTA:F2:CATC -GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCGGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG -+ -GGGGGGGGGHHHGHHHFHHHHHHGHGHHHHHHGHHHHGGGGHHHHHHHHHFFHHFHGHHHHHHGGHGGHHHGHHHHGGHFHHHHHHHFHHGHHGEFFHFHHGGGGGGHHHFHHHHGHHHHE?FGHHHHGFGHGHHGGGDGGGGGFFFCFFFFFFFFFFFFA;DBBDFFFAFFFF;DBAFEDFFFFFEA?-9...9;9;BFFFF//BFBFFFFBFBFFFFFB/BFFF9B/EDEF-BFF. -@M01368:8:000000000-A3GHV:1:1107:22864:15712/2:R1:AATAAGAC:R2:GTAATATT:F1:ATCA:F2:GAAC -TAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTCTGTCTCTTATACACATCTGACGCTGCCGCCGAGGGCTCTAGTTGTGGTCTG -+ -GGGGGGGGGGGGHHHHHHHHHHGHHHGGGGFHGHHHHGHFGFFFFGGHGGGGGCHFHFAFEDHHGGFGGGHHEGEGGHHEGGCHGHHHHGGGGGGHGF0EECGGGGFECCFFGFBGHHHHHHGGHHGHFCFEHFBEFGGADDDC=CC0CCFHG:CEHHHGHBGH:GFFGFF0CAAA/BF09BBBBBBB9CFFB0C00;000;:CFFB;:...D?>:@=:9-@...//;////..;/;/ -@M01368:8:000000000-A3GHV:1:1107:24817:14233/2:R1:CTTCAGGG:R2:CTTTAGTA:F1:TCAT:F2:AGTA -GTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAAAGTGTGGGCTATTTAGGTTTTT -+ -GGGGGGGGGFHHHHHHHHHHHHHHGHHHGEHGGHHGHHHHHGHHEHHGGGGEHGHHHGEHHGHHDGHHHHGGGGGFGEFGGEGHHGCEEEGHHEFGGDHGGGGAGFHHHHGGF/?//GHFFDGGGGGHAD??DC1DHHBGHHHHGHF<0=GGHGCCFGHGBGGD0GGHC0C.@?ACEEC/.EDEB?BFFCFB9CFF090BBCBFBF0CCB0B00A.9E.B/9BA=-..B9FBF//;BBBBFFB:./9/9/9;B//9BF/;B/ -@M01368:8:000000000-A3GHV:1:1108:11059:23181/2:R1:GTTATTAT:R2:AACTGTAT:F1:TATG:F2:CCGA -ATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACC -+ -GGGGGGGGGHHHHHHHHHHHGHHHHHHHHGHHHHHHHHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHHHHHHHFHHHHHHHHHHHHHHHHGHGHEHHGFCGGGHHHHHHHHHHHHHHHFGHHHHHGHGHGHHGGGBFGFFFFEAFFFA.BFFFEFDDFFFFFFFFFFEEFF;D;EFFFFFF0BFFFADDFFFFFBFBFFFFFBB0FFFF0::0BF000BFFFBBF0EDFF@ -@M01368:8:000000000-A3GHV:1:1108:12233:13498/2:R1:TAGGTGCG:R2:GGTTCCTA:F1:ATAA:F2:CTTC -GGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACTGTCTCTTATACAC -+ -GGGGGGGGGHHHGHHHHHHBGHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHGHHHHHGGHHGHHHHHHGHHHHHHHGGHHHHGGGGGHHHHHHHHHHHHHGHGHHHHHHGHGHGGHGGGGAGGFGGGGGGGGFFFFFFFFFFDFFFFDFFFFFFFFF>.AEDEFFFFF?E-DAB9FFFFFFFEF:FBFB009:0FFFF9BFFBFFFFBB0.9A=-/0BFF0BBFFBFF:90: -@M01368:8:000000000-A3GHV:1:1108:14328:14751/2:R1:ATAATAGG:R2:GTATCCGA:F1:ATGA:F2:CATC -GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATCTGTCTCTTATACA -+ -GGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHDHHHHGGGGHHHHHHHHHHHHGHHHHHHHHHGGHGGHGGHHHHHGHHHHHHHHHHHHHHHHFHGHHHHHGGGGGHHHHHGHGHHHHHHG?FHHHFHGHGHGHHGGGGGGGGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFAFFE.ADEFFFFFFFADFFFFF///BBFF/FFFFFBFAFFFFBB///;9:BBFFFBF9B9//:/ -@M01368:8:000000000-A3GHV:1:1108:16350:22621/2:R1:TTAGTAAG:R2:CATCTGGT:F1:TATG:F2:TCCT -CTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATAT -+ -GGGGCGGGGHHHHHHHHGHHHHGHGHHHHGGGGHHHHHHHHHHHIHHHGHHHHHHGGHGGHHHHGHGHGHHHHHHHHHHHGHHHHHHGGHHHHGGGGGHHHHHHHHHHHHHGHGHHHGHHGGGGGHHGGGDADDGFFGGGGFFFFFFFFFDFFFDFFFFFFFFFFFAFAEFEDFFFFFFEE-AAFFFFFBFFFFF0FFFBFF;AFFFFFFFFFFFFFFFFFFFFFAB9BFFFFFBFFF -@M01368:8:000000000-A3GHV:1:1108:21791:23272/2:R1:CTTTAGTA:R2:CATAACAC:F1:AGTA:F2:TTGG -GGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCT -+ -GGGGGGGGGHHHHGGHHHHHHHGGGEGGHHFGDHGHHHHHHGGHHH2GGHHHGHHGGGGFGFBFHHHGGGGHGHGHHHHHHHHHHGHGHHGHHGGHGGHHHHGGFHGGGHFHGHHHHHGGHHHGHGFCGFFDGGG/FCFHBGHHHHHHHGHCHHHHGGGHGHGHGCFF;>@DFFBFFFFFFDFFFFFFFF;;.DDAA;FFF;BB;C@..9::BBFBBA;-:.::FBB///;/////:/ -@M01368:8:000000000-A3GHV:1:1108:23314:26089/2:R1:ATTGAACG:R2:CATAACAC:F1:TAGG:F2:TTGG -GGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCC -+ -GGGGGGGGGHHGDHHHHHFHHHHGGGGGFHFFGFFHEGHHHHHHHHFGHFHHHHDEHHHHHHGHFHHGGGGHHGFHHHHHHHHHHHHHHHHHHF?EEFCHFGFHHEFFFHGFHHGHFHFHHHFFHAG??GHACGGDGHGGBFHHHHHHHGHFGHGHGEGHGCFFF@CD-BDDFFFFFFFFDDFFBBFFD-B-A.DFFA.AFEFF-;-..:..BFFFFFA-../9FFF//B/BBBBFF/ -@M01368:8:000000000-A3GHV:1:1108:25084:18127/2:R1:TTATTATG:R2:CCCTTAAA:F1:TCCT:F2:TAAG -CATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAG -+ -GGGGGGGGGGGHHHHHHHHFHHHHHHHHHHHHHHGGHHGHHHHHGGGGFHHHHHFHHHHHHHHHGGHHHHHCGGHGHHGGGGGGGHHGGGGGGGGHHHGHHGGGGGGGGGGGGGGGG@CDE?EFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFFFE;DFBFFFFFFFFFFFFFFFFFFFAFFFFFFFBFFE0BBBFFFFFFFFFFBFFFF9BB0:0:B; -@M01368:8:000000000-A3GHV:1:1108:7626:10048/2:R1:ATCAAAGA:R2:AACCTAAA:F1:CAGA:F2:TAGC -CACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGGAGATCTCTGGGGTCGGCGGATTT -+ -GGGGGGGGGHHHHHHHHHHHHHHHHHHHGGGGGHHHHHHHHFGHHHHHHHHHHHHGHHHHGGFHHHFGGGGHHHGHHHHHHHHHHHFGHHHHHGHGHGHHGCGCGGFHHHGGGGGGGGGGGGGGGGGGGFFFAFFFFFFAA-AFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBBFFDFFFFFB-<>;>@BE<:000.0.0/0<00<0:>..-<<<---.0 -@M01368:8:000000000-A3GHV:1:1109:11270:12255/2:R1:AATCAAAG:R2:CTACCTCA:F1:ACAG:F2:GGGT -ATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGGAGATT -+ -GGGGGGGGGHHHHHFHHHHGGGGHHHHHHHHHHHHHHHHHHHHGHGGHGGHHGHHHHHGHHHHHHHHHFHHHHHGHHGHHHHHGGGGGHHHHHHHHGHHHHHH?FHHHHHGHGHGHHGGGGGGGGGGGGGGGGGGGGFFFFFFFFFFFFFFFFECADFFFF.DEBFFFFFF;D.B;.ED.FFFFFFF-.:00009BBF -@M01368:8:000000000-A3GHV:1:1109:24217:19077/2:R1:AAAGTGAA:R2:TAATAGGA:F1:CTGT:F2:TGAG -CAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGAACCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTTCTGTC -+ -FEGGCFGFGHHHHFHHFHHHHHHGGEEEFGHGFFAGFFEEAEGGHHBEEFAFGAEF1G3GFGGEEF@EAGGHGFF?ECGGHEEEFGGHHHHFHHFHHGFHHHC2DAGHGG00-BFFA9FFFFFFFFFFFBFAFFAFFFFFBFFFFFFF/9BFFDFFDDFF-9@CDAD;:AFF/;B//....;;///; -@M01368:8:000000000-A3GHV:1:1109:27103:7153/2:R1:GTAATATT:R2:NTACTTCA:F1:GAAC:F2:GGGT -NTAAAACCTNAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCANGGNAGCTCTCCNNGNNNNTGGTATTNTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACCTGTCTC -+ -#AAFFGGGG#BAFFAGHHHGGGGHGHHHHFHHHHHHHGHHHHHHHEGFGGHHBGHGHHBGFFEG2GHHFHFHHHGHHGHFH#/B#??FFGHHH##?####????FHG#??CFGGGEF>CGAGAGGGFGAA@@FFFFFFFFB=@@FF?FF;FFFF@@-@-AFEF?BB/BF?E-BBFFF;:BB/BFFFB/BFFFBFBFBBFFFFFB/FFFBB/;9A@@BFFBFFFF/BBFFFFFFE/:9/ -@M01368:8:000000000-A3GHV:1:1109:8763:7859/2:R1:TAGTAAGT:R2:GGTTCCTA:F1:ATGT:F2:CTTC -GGGTCATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAG -+ -GGGGGGGGGHHHHHHHCHFAFHHDGEGFHHHHHHHHFHHHHFHHHHHHGHGGHGGHHHFHGHHHHHFFHHHHHHBGHFFHGHGHHHHHGGEGGHHHHHGHHHHHHHHHGFGHHHHGHGHGHHCCGGGGGGGGGGAAGF?FFFFBFA@=BDDFF?-;FFF;@CFFEFEFFFFFFFFFBFDFFFFFFFFFFFFFFFFFFFFFF:FFFF/BF/FFFEFF?DECAFEFFFFFE:FFFFFFFB -@M01368:8:000000000-A3GHV:1:1110:12808:18367/2:R1:AATTAACA:R2:GCCCACAC:F1:CACT:F2:GTTC -CCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACAGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTCTT -+ -GGGGGGGGGHHHHHHHHHGGGGGGHHHHFHHGHHHHHHHHHHHHHGHHHGGHHHH3GFHEFHHHHHGHHGFHHHHHFGHHHHHGHGHGGHGGGGGGGHHHGGCGGGGHHGGGGGGGGGGGGGGGGGGGF-BAEFEBEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFFAFEFDFFFFFFFFFF0BFBED=@DFFFFFFFFFEFFFFFBFAFB;0:;F0 -@M01368:8:000000000-A3GHV:1:1110:14751:26540/2:R1:TAACACTT:R2:ATGTTCGC:F1:GGGG:F2:CTGT -ATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGGTTGGGCTATTTGGGTTTTTTGACCCTGAAG -+ -GGGGGGGGGHHHHGGFHGGHGGCHHHHHHGHHHGHHGEGGGHHHHHHHHHHEFHHHHHHGEE?FGHGEHGGHHHGGGGGGEHHGGGGHHGGGEGHHHGHGGGDFGHHGHGGGGGCGAFFCCDFHHHH1CGGHBHFHFCGFFCDFHGCCE.CC:EGFHHCGHHGCF?FGGGFGGG;B9;FFFFFFEGEBF0;;;0;CFBFFF?D.DE.:..99-...;;//9A.;DE.9-./:FB.;// -@M01368:8:000000000-A3GHV:1:1110:15519:10008/2:R1:CTGTAATA:R2:GGTTCCTA:F1:TTGA:F2:CTTC -GGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAG -+ -GGGGGGGGGHHHGHHHFHHBGHHHGGGGHHHHHHGHHHHHHHHHHHHHHHGGHGGHHGHHHGGBGHHHHHHHHHHHHHHHHHGGFHHHGGGEGHHHHHHHHHHHHHHHGHHHHHHGHGHGHHGGGGGGDGGGGGGGGGBFFFFFFFFFFFFFFFFFFFECB-=BFFFFFFFFBB?E-A::FFBFFFFFBFFFFFFFFFFFFBFFEFFF;0BFF0:FE?FFFFBFFFFFFFFFFFFFF0 -@M01368:8:000000000-A3GHV:1:1110:16559:7507/2:R1:CATTCAAT:R2:GCCCACAC:F1:TGTT:F2:GTTC -CCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAAT -+ -GGGGGGGGGHHHHHHHHHGGHGGHGHFHHHHFHHHHHHHHHHFHHGHHHHGHHHHHGGEEGHHHHHHHHHHHHHHHHHHHHHHGHGHGHHGGGGGGGHHGGGFGAGFGGGGGGGGGGGDDDFAGGFFFFCFAFEEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFFFFFFFFFFFFFFE..@BFFBD./;:0FFFFF09B/AFCA=-99BFFBFFFFFFFB:BFBFFBFB9: -@M01368:8:000000000-A3GHV:1:1110:16700:6843/2:R1:AAATAATA:R2:GTTCCTAC:F1:GGAT:F2:TTCA -GGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTCTGTCTCTTATACACATTTGTCGCTC -+ -GGGGGGGGGHHGHHHGGHFHHHGGGGGHHHHHHHHHHGHHHHHHHHHHHGGHGGHHHHHHHGBGFFHHHHHHGHHHHHHHHGHHHHHGGGGGHHHHHHHHHHHHHHHFHHHHGHCG/GGHHGGGGGGAFGEEADAGGDFFFF00D>CFDDFFFB?AF?B;-@>.FEDFFFFF0BF-BDFFFFE00B0BF0FFFF:BFA0B0:BBFBBFFFFF0B0:000<00FDFFFFFFFFFFFBFFFFFFFFFFF/FFF/BFFFFFFFBA.?@;FFBDFFFF/ -@M01368:8:000000000-A3GHV:1:1111:7437:24508/2:R1:ACACACTT:R2:ACATCACG:F1:TAGT:F2:ATGG -TCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTCTGTCTCTTATACACATCTGACGCTGCCGAGA -+ -GGGGGGGFGHHHHHHHHHHHHHGHHHGFHHHHGGGGDHHHHHHHHGFHHHHHGHHHHHHGHFHGHHGGGDFGDFFGGGFFGFFGHGHHHGGC?AD-:A-AFFGGG-?9EFEFGGFFBFEFCFFFFFFFBBBFFFFFFFFFFFFFFFBBFFFFFFFFFFFFFFFFFFEFFFFFFBFFFFEFEFFFF;CF-FFFFFFFF/BFFFFFFFFFFFFEFBFFFB/FFFFFFF//9A..;F--@9 -@M01368:8:000000000-A3GHV:1:1111:8665:25542/2:R1:TCCTACTT:R2:CTTTAGTA:F1:CAGG:F2:AGTA -GTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGACAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTA -+ -GGGGGGGGGHHHHHHHFHHHHCHHGDGGGGHGGHHHHHHHHGHGHHHGGGGGHFHHHHFHHHFFHHHHHFGCGGEHFHHFGGHFHGGGGGGHHFEEEAFEFFDFHGHHHHGGGGGGGHH0DGGGGCGHCC-DFFF. -@M01368:8:000000000-A3GHV:1:1113:5741:16959/2:R1:TAGTAAGT:R2:GTATCCGA:F1:ATGT:F2:CATC -GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG -+ -GGGGGGGGGHHHHHHHGHHHHHHHHGHHHHGHDGHHHGGGGHHHHHHHHHHHHHHHGHHHHHGGGEGGHHHHFHHHHHHHHHHHHHHHHHHHHHHGHHGHHGGGGGHHHHHHHHHHHGHHHFHHHHHHGHGHGHGGGGCGGFGGFFFFFFFFFFFFFBFFFFFFFFFFFFFFAFFFFEAEFFFFFFFFFFF9BFFBFFFFFFFFFFFFFFBFFFFFFFFFFFFFFFFFFADAB-/BF. -@M01368:8:000000000-A3GHV:1:1114:10130:11959/2:R1:CGTTCCCC:R2:ATCAGAGA:F1:TTAA:F2:CAGA -ACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGGAGAGTTCTGTGGGGCCGGGGATCCTTATAAAAAAAATAGA -+ -GGGGGGGGGGHHHHHGGHHHGGGCGGHGHGGGGHHGGGGHHHHHHGGGEGGHHHFGGGGG?E1FE?/EEHHHHHGHHGHHHHGHFHGHGHHGDGGFG2FF2?GHHHHHGCCCFHGHGHHHHGHHFEHHFGHHGHH<1=DGHHHGHHGHGAGAEEDG.CGCGHC0CGBFHGFBBF0ABDDEFF@?--:BB@.;:BF;0.0:0.0:000:BF.-.------.9/;000::0;0:--.000 -@M01368:8:000000000-A3GHV:1:1114:14540:5315/2:R1:CTTTAGTA:R2:CACTTGGG:F1:AGTA:F2:GGTA -CTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCC -+ -GGGGGGGGGHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHHGHHHHHHHGHHHHFHFHHHGGGGGHHHHHHHHHHHHHGHHHHHHHHGGGGGGHHHFHHHGHHHHHHHHHHHHHHHHHHGHGHHHGGGFGHHHHHHHHFHHHHHF?1FHHGHGHGHGHHGGFFFFDBFBE;BCC.:BFFFFFFFFFFFFFF;AFFFFF-=-.AEDEFFFFF..9A;9FFFF0FFFFE00FFF0:BA. -@M01368:8:000000000-A3GHV:1:1114:15066:16302/2:R1:TAAATAAG:R2:TTATTATT:F1:ACAT:F2:ATGT -CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGTGG -+ -GGGGGGGGGHHHHHHHHHHHHHHHDGHHHHFHHGHHGHHHHHGGGGEHHHHFHHFF5FHHFEGHHHGDHGGHGHGFGGGEHFGHHGGGGGHGHHHGHHFHHB3FGHHFGGGG?GFFHCCEBGFFECCDFEGFCFGCHHGFDDHHHGHHCFGGGGGFBFDGFG?-:..AFG.-C0C009;:00;00:9/:CEFFF?AE::9;9?0:FEF0;0..--./00::B/090000000;A.... -@M01368:8:000000000-A3GHV:1:1114:16639:15258/2:R1:CCTAAATA:R2:TTATTATT:F1:GCCC:F2:ATGT -CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCGCACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATG -+ -GGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHGHHHHHHGGGGGHGIHHHIH5DEGHHHF?FGHGGHGGHEGGHFHHGHGEHHGGGGGFFFGHFBG2GHEBGHHGHGGEG/GFGABEDFGHEED?GGHHFFGGGCFEGD/GFHFFGEFGCGG?CC??D-EF@EEEFGCDDBBFGGGEBBFFF09090A.BFGA.9CCA0;EBAB00BBFF.@-./;BB;BFFF0:00099AAFFF0 -@M01368:8:000000000-A3GHV:1:1114:2404:13066/2:R1:TCCTACAA:R2:ATGGATCA:F1:GCAT:F2:CAGG -CTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGACCATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACTGTCTCTTATACACATT -+ -GGGGGGGGGHHHHHHHHHFFHHHHGGGGGHFFFHHFHHHHHHHHHHHHHHHGFEGGGHGEDFCDFHGHFG@@DGGHHHHHHGGGGCGGGGGEHGGCGBB?CF99EGFGGFGG?D9CFFFF/BBFFFFFEF9BFFAFFFFEFFFFFFFFFFFFFFFFFFFFF.FFBBFFFFFFFFFFFF-9;;;BFFFFFB9BFBFBFABFFEFFFFFFFFFF::BFFBFFFF.9//;FFFFF/BFFB/ diff --git a/tools/trim_galore/test-data/paired_collection_example_pair1_results3.fastq.gz b/tools/trim_galore/test-data/paired_collection_example_pair1_results3.fastq.gz index ee71a9bc91..ff0dc41d36 100644 Binary files a/tools/trim_galore/test-data/paired_collection_example_pair1_results3.fastq.gz and b/tools/trim_galore/test-data/paired_collection_example_pair1_results3.fastq.gz differ diff --git a/tools/trim_galore/test-data/paired_collection_example_pair1_results3.fastqsanger b/tools/trim_galore/test-data/paired_collection_example_pair1_results3.fastqsanger deleted file mode 100644 index 0d55c14fd4..0000000000 --- a/tools/trim_galore/test-data/paired_collection_example_pair1_results3.fastqsanger +++ /dev/null @@ -1,392 +0,0 @@ -@M01368:8:000000000-A3GHV:1:1101:14518:9998/1 -GTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTG -+ -AAAAAFFFFFFFGGGGGGGGGGHGGHHHHGHHHHHHHGCGHHHHHHHHHHHHHHHGGGGGHHHHHHHHHGHHGFHFE5BGEEHFGGGHHHHHHHHFBHHGGGGFHGHHFGHHHHGHHHHHHGEGGGGFHFHGEGHHGGCDGDGHGGGDGGHGGCGGGHGHHH/ACDG?.1FGCDCCGCA.CC@CDCHFHGFFGGGEBFGAB//CEFBFGG.:;D;;A0AFFFFFB..:@ABFF//;BFFFFFBF/9D:A -@M01368:8:000000000-A3GHV:1:1101:18422:19051/1 -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTA -+ -CCCCCFDDDDDFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHFHHHHGGGGHHHHHHHHHGHHHHHHHHHHHHGGHGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHGCGGGHHHHHHHHHHHHHHHHHHHHHHGFDHGFHCFGGGGFGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF;FFFFFFFFFFFFFFFFFFFFFFFFFFFFEFBFFFFFFFFFF:FF -@M01368:8:000000000-A3GHV:1:1101:25545:21098/1 -ATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATAAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGGCTTATTTAAGGGGAACGGGTGG -+ -33AA?DFD5BDFGGGFEBDGEGHEGHGEGHCEGGHHCHGHHFFHHGFGAGE53FF2FAFFGDE5FFFE5GFBFGAEE1GHHHGHHHEHE3FGHF@GEGEGGHHGG3FAGFFDE?EEE3GFEGFGFGGCG?GHHHFHGGGC@DHFFHD/AA@AAAAD2ADDFFGGGGGFGGHA?EAEFBEAGHFABFGG5FDF5DB1EEGAFDFB53FF5FH@G5FFEHGHEFHFFHBE333GF43GCGGGGE@0?BFGGB0B?FHGFDGGHHHBFFDEGGHGFFFDFE@<1>@FFFGHHHHFHEFGDABFFGG/@DCE;>=EF?AEFFFF;9;=DAFFFFBBFFFBFFF//;BFB.:FBFFBB/BFFFF/BFFE?EFAFEF9 -@M01368:8:000000000-A3GHV:1:1104:11928:24197/1 -ACGTAGGTGCGATAAATAATGGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATA -+ -CDDCCFCFFFBCGGGGGGGGGGHHHGHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHGGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHGHHHHGHHGHHHHGGHGEEGGGGFHHFHHHGHGEHHHHHHHHHHHHHHHHHGGGGGGFGGGGGGGGFFGGGFGFGAEFEFGFEAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF@D9 -@M01368:8:000000000-A3GHV:1:1104:12288:20635/1 -CACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGCCGCAGCATCTGTCTTTGATTCCTGC -+ -ABA?AFBAB2>AFGGGGFFGD5GGHGFFHGHEGGGGHHHHFFHH5FHGHHHFFHEFHHHHHHGGHHHHHGFHGGHGGGGFHGGGFBHHHFGEGHHHHGHGGGGECEHHFHHGHHFFHFGGGHHHEGHDGHHGHGHFHHHECGCBCHHGFHHFHD1GFHEFHEF?GDCFFEFE0A@;@>BD/9BFBBBBFDFFFF99D=?>DDBFF-AFFEBB9BBEFEFAFFF//-@---;;FBBFBFB///;99999B -@M01368:8:000000000-A3GHV:1:1104:13832:4398/1 -CACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTC -+ -AAABABCAFFFFGGGGGGGGGGHFHHHHHHHHHGFFHHGGHGGGGGHHHHHHHHHHFHHHHGHHHHHGGHGHGGHGGGGGGGHGFFGGGGDGGHHHHGGGGFGGGGGCFGGEGCDGGGGGGGGGGGGEGF?;BFFFFFF -@M01368:8:000000000-A3GHV:1:1104:23670:4161/1 -ATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATC -+ -AAAABFFFFFFFGGGGGGGCGBBGGHHHHHHHHHGHHHHHHHHHHFGGHHHHHHGGFHHHHHHHGGGGGFHHHGHHHHHGHHHHGHHGGGHGGGGHHHHHGHHHHHHEGGGFHHHGFFDGEGHGHFHGHHGEGGGHHBGHGGHGHGGGGG/@FCDFDGFHGGGDFFHFGFFGGGGGHHHHGGGGGGGEGCDG?GGBFFFEE00BFFGABBBAFGGGEGEA.9FFF/./;BFBF./AAF/BFFBBF/F/BF -@M01368:8:000000000-A3GHV:1:1104:24927:22253/1 -AGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTAT -+ -AAA?AFFF3DFFFGGBGFF3EAEEEHFHHFHHHGHHHHDGHHGAEFGHHHGFCHHGHHHBGHHHAEGGHGGFHFBFHHHHHHHHHHFHH1EEHEEHH1FFDEEFCGHBGHHFHEHHHHHH1FCGHHHHEEFGEEGFFCHHH11>GHHF/FG2DGGCGAGGHGGGG@@@BEFFF?@@@FFEFFFFFF?@@FB@BB@FFFE=;==---A9EFBBB/;E;A9B/B/FFFEF/B///;/;9/A/;/9BFFFBFBB -@M01368:8:000000000-A3GHV:1:1104:4705:7727/1 -CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG -+ -BBBBBFFBFFFFGGGGGGGGGGHHHGHHHGHHHHH6GHHHHFFGGGHHHHHHHHHHHHHHHGGFGGGEGGGHHHHHHHHHHGHE3FGGGGHHHHHFHHHHHHHHHHHHGGGGGHHFHFCGFHHGGGGGGGHHGGGGHHGGGGGGHHHHGGGADCHHHHGDGGGGGGGGC@DGFHEHHHHHHGHHHHGFGDEGFGFFGGGGCFGBEGFGGGFG;C.BFFFFFFFBFBFFFF/F?.AFFF/BFFEFFFFFEFF -@M01368:8:000000000-A3GHV:1:1104:7756:4776/1 -GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTG -+ -CCCCCCFFFFFFGGGGGGGEEFHHHHGHGHHHHHHGGHHHGGGGGGHHGHHHHHGHHGHHHGGFHHHHHHHHGHHHFHHGHHGFGGGGHHHHHHHHGHGHFFHFHHHHHHGGHGFGFHHHGHG?FGHHHHHHHHHHHHHHGHGHHGHGGCDGFFHHHHGHHHHHGHHHGFHGHFHGHGHGH/@CABFFFFFFFACAFF?BBFFBFD-@-.D>FFAF.F.:9---;AADFFFFFBEFFF:9FFFFBBFFFF -@M01368:8:000000000-A3GHV:1:1105:11310:21761/1 -ATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTT -+ -CCCCBFFFFFFFGGGGGGGGGGGHHHHHHHHHHHHGHHHHHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHGHHHGHHHGHGHHGHHHHGGHGGG=FFGEHHHHHGHFGGHHGHHHHHFHHHHHHHGGHHHHHHHGHEHHGHHHHHGGGGF9AGE?DEGFFGGGGGGGGGGGGGGFFFF/9FFBFFFFFFFEFFFBFFFFFFFFFFFFEFFF -@M01368:8:000000000-A3GHV:1:1105:11578:14676/1 -ATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGT -+ -CDDDDFFFBFFCGGGGGGGGGGHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHFGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHHHHHHHHHGHHHHGGGGGGHGGHGHHHHHHH;CHHHHHHHHHHHHHHHGHGGGGFGGGFGGGGGGGGGGGGFGGGEGGFFFFFFFFFFFFFFFFFFFFFFFFF/FFFFFFFFFFFFFFFF -@M01368:8:000000000-A3GHV:1:1105:13575:19255/1 -TTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGTTCCCGTGAGTGGTTAATAGGGTGATAGGCCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG -+ -AA1A1DFFFFFBGG3A111FFAEGDDBGFDHF00F01BBA00BFGEHFBFFA1FABB0FAE?CFHF1BGFHFB0011F121E//>>1F10>B?EFHGCC/?/BFG/EEGFFEE//BCFDE0?E@@B/?11AAFFFFFEAGGGFGGAA6FHDGGCGFHFFHFHFHHHHHGDDDFFFHGFFHGBEGEGHHHFFFHGEEAEGHHHHHFHHHFHGHFEDGGEGBEAGEFHHFFHHHHHHGGHHHGHHGBGHHFHHFGHHHHG?CG?AFHH1FHHHGHFHHHF?DGHFFHCGAGEFHGCBBBB?BFFF?B-B.9/BFFFFFB=-AD?..@FFFF@@-@@?AFD.FFFBBEEBD9/:FFFFFBB/:FF9BF/BF.BBFF/ -@M01368:8:000000000-A3GHV:1:1105:21099:2895/1 -TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATT -+ -CBCBBFFFFFFFGGGGGGGGGGGGGFHHHHFHGHHHHHHFHHHHFGFHHHHHHGHHHHHHGHHEHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHHHHHHHFHHHHGHGHHHGGHHHHHGGHFFGHGCGGGHGHHGHHHFGHHFHH/FGGGHFGFEGGCFCCD;B@DFFFFFFFFFFFFFFFFFFFFFFFFF;F.BEFADFFFF.AEFFFFFFE;D.;:B00099:09BFBFFBFF.0:000BFFBF -@M01368:8:000000000-A3GHV:1:1105:26469:19245/1 -TTATGATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCAAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATG -+ -BAAAAFFFBFFFGGGGGGFFGGHHHHHHHHHHHHGGHHGHHHGGHHHHHHHHHHHDEGGHGHHHHHGEDHFHGFHGGHHGGHGGEHHHGHHGHHGHHHGGEGGGHGHGHHHHHHGHHHHGHGGGGGHFHGGGGHHGGGGCDGHHHGD>A@FFFFFFFGGGFEECFGGFFB5GBHH5DFH5GBEEAEFHFHHF3F3GHFHHHEF3GAEHBGFHFFEFFHHHHFFB31FGGEGFHGEHHHHHHHHHHHHHHHHHEEEEGHHHFFF3GF3FGF44BBGEHHHHGG2B/BGGHE;B=.9.;.BBFFF..;A;/B/;B//;;//BBF -@M01368:8:000000000-A3GHV:1:1106:22927:3863/1 -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGACTT -+ -A>ABAFA?D@AFGGGGFGGGFGFHHHFFH4BCBGHHHHGFFF3G5BF3322EEAFHHHHHHAB5GHFHEHFHHH3BEEHFGF5@13FDF3GDGFHHFGHHHGHHHGFGGHHFCG//?/EGHHHG3F4BFFGHHHEDGFHHB/B/?FBF<@-9?BDEFFF?B--?DFFFB9FA>B;A.BFDB?AB.--@;-.FFAEFFFF9AE;DDFFFFB/9BF -@M01368:8:000000000-A3GHV:1:1106:23625:4236/1 -TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATT -+ -@?AAAFFBFBFFFGGFGFGGGEFCGGHHHHFGHGHFHGHHHHGGFGGFGHHGHGHHHHGHGFFHHGGGGGHHHHHHHHHBFHHEFHHHHHHGF?FAGHGFHHHGDGFHHGGGHGHFHHHGHHHGHHFHHGFFGFHHHGHHHHFFHHHHH/FGHHHHGHGHGBFFF9 -@M01368:8:000000000-A3GHV:1:1107:14451:26748/1 -GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGAC -+ -BABBBFFDABBBGGGGGGGGGGHGHHHHGAGHGGGGGHHHHHHHHGHHHHHGGGGGGFFHHHHHHHHHFHHHHHGGGGCHFHH3FEHHGCGGGDGHHHGFGGHHGGGGHHHHHHG/DGGGHHHHGGGGGCCHGG?CGG1GHHGHHHHBGHHHFHHGHHHHGGADAD00ECEGHEFHHFFAEFFFFFGGFBFBFFFGFFG.;B/99;BCFGGGGEFFFFFFEDA./:ADEB?FBFFFF;FFEBFBFF -@M01368:8:000000000-A3GHV:1:1107:18714:24788/1 -GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACC -+ -BBBCCFFFDEDEGGGGGGGGGGHHHHHHGGHHGGHGGHHHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHCFGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHGHHHHHHHHHHHGHHHHGGEGEGCCEGGHHHHHHG:CAFFGGGGGGBFEFGGGGHGAFFGGFGGGGGGGEFFFFFFFFFEF/EDDFFFFFFFFFBFFFFFBBFF -@M01368:8:000000000-A3GHV:1:1107:21355:8333/1 -ATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATG -+ -ABCBBFFFFFAFGGGGGGGGGGHHHHHHHHFHHHHHGGGGGHHHHHHFHHHGHHHHHHHGGGGGHHHHFFGHGHGGGGGGGHGGEGGGHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHGHHHFHHHHHHHHGHFFHHHHGGHGEGHHGHGFHHFHGHCEFHEGHGHHHGHHHGGGGGGCFFFFBFGGFGGGGFBG?FDGGGEGFFFFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFF.AA/9 -@M01368:8:000000000-A3GHV:1:1107:22864:15712/1 -AATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTAC -+ -BBBBBFBBFFFFGGGGCEGGGGHGHHDGFHHHHHHHHHHHHHCHHGHFGBGDFGE?GHHGHFBHHFHHHHHHHHHGHFGFGHGHHGGC@DCFGHHGGGGGGGHHHHHHGGGGGFGGGGFFGGGGFADGFEFFFFFFFFFFF9;FFFF/BFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFEAECDFFFFFFFFFBFFFFF -@M01368:8:000000000-A3GHV:1:1107:24817:14233/1 -CTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGA -+ -3AABABFFBFFFGCFGGGGGGGHHHFGCFGGGFFGHHCGHHHGHGHGHBFFHHHEDGGFGFGHHFFFHGGEGHFHHGHFHGFFHHHFGHHHFHACECEHHHBGFHGFHHHFHHF4GFHFHFHEHGGE/EG:A-CFGGFGADGGGGFGGGGDAAAADFA.-AFFECFDAAFFFFEFFFFBFF;D;BFFFB/;BFFFFFFFFFFFFFFBFFBF/;//;BFFFF?FFFFFFEDAFFFFFBF//:BFBACF -@M01368:8:000000000-A3GHV:1:1107:24830:14440/1 -AACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCA -+ -ABBBA@FFFFBBGGGGGGGGGGHGHHGHHGHHHGGHHHHHGHGHHGDHHHHHHAGGGGFHHGHHHHHHGHHHHHHHHHHHEGAFGFHFHHHHGEGHHHHHHHHHHHHHHHHHGGHHHGEFEGGHHGHHHHHGGHGFHH/?GHHHHGFGHGFHCGGBBDAFFFEFFFFFFFFFFFFFF=CDDADDFFFFEF=BFFFEFFFBFFFFEEBF./:FFFFFFFFFFFFFFFFFFFEFFFFFFFFFFF/BFBEFF -@M01368:8:000000000-A3GHV:1:1107:26478:23602/1 -GCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGAT -+ -1AAAAF1B13BBBEGGGFGGGGGGHHDB1BGFHHHFGFGGG0F00BGBGFGGF1GBGB1BGCGAEE/FGFHGHFHHHHHBFF1B0FGGF//F//FGFGFFF/1@B1DFHHHGGEFEDHFDFAFF0B//1FDD1GFFHHDF?1BD>GF/GCAC0C@?@-;99BBB->@--;-B//99B--9-BF=A--A-:A----9AA9AFBFBBB?E@BBFFFFFFFBFFFFF -@M01368:8:000000000-A3GHV:1:1108:11059:23181/1 -GTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGA -+ -CCCCCFFFFFFFGGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHGHHGGGGHIIIHHHHHHHGHHHHGHHHGGHGGHHHHHHHHHHHHHHGGGGGHHGHHHHHHHHHHHHHHHGGGGGHHHHHFGHHHGGGGGGHHHGGGGGHGGGGHHHHHHGGGGGGHGGHEFGGGGGHGGG-ECHHHGGGGGGGGGFGFFGGGGFGGGCF?EFGG0;BFFFFB?9AAFFFFFFBF;BBFFBFFFFE?F -@M01368:8:000000000-A3GHV:1:1108:12233:13498/1 -TAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACC -+ -BCCBAFFBCCCCGGGGGGGGGGHHHHGGGGGHHHHHHGHGHBGFHHHHHGGGGGHHGGBFGHHGGGGGGGHHHGGGGHHGGGFHHHHHHGGGGGGHGHHGGGGGGGGGGGGGGHHHHFHHHGHGHHHHHHGHGHHGGFGGGDFFGGHHHGHGH/FHHHFHGHGHHGHHHGHHBAAAA3BDFFFFDGBFFEEGGGGHHGHFHHCFHGHGHHHGF3BFGFHGFEFHFHGH3EGFC1EEGBFGBHFGGFFHHHGHFHGHHGHGGHCEHEBCFF3ED3@GH4FDEG12FFHFFHG/G3FGA/@EFEFCG33FFGBBGHFHH/?BFBGFGFAGC2C/@-;@-;BFFEFADF?DFFFFFF?;AFFA;AD;..FEFFA--;9;D.9BFFBE?;.;///://///99//BB/9;F./B//BBFB9// -@M01368:8:000000000-A3GHV:1:1109:25237:11722/1 -CCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATG -+ -AABBBFFFFFBBF5BGGFGABGHGGAEFGFHHHHFHCHFHHBFGGGEHBGGHFFFFGFAEF5FHH?EGGF5AEG3?EBFHD?C/EFHFFEGEE?CFGGGHHFHB3EEFG?EEGHCFGGGGGGGECA?FFFDFBFGCG4EGFG??GACFGGHFHCFHCHBECDEFCEGHHHHHFFGGF3AFFHHFGEGCGHHGEHGFGHHEGG?EFHHH?EGGHHGGGGHHHHHHEGGGGGHFHHGFDDGGACFCC@?/FDDGHHHGHEFFHHH0FDHHFFEDCD.CFHHGDFGGGGGEFGC?EGHHHB0CGHHGCGHHHHBEHGHH.::ACA;FECFGGGGGFFFEFGGGGGGGFFBBBFGBFFFEFFFFBFFFFFFBBFBFFB9..AF?FFFFF -@M01368:8:000000000-A3GHV:1:1110:14751:26540/1 -TAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACTGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGTTGTGCACGCGATAGCATTGCGCGACGCTGGCGCCGGCGCACCCTATGTCGCAGTATCTGTCTTTGATT -+ -AAA?1CFFFFADGEEGFGB1112DGDGHGFGHHCG/ECEBGGHDFGHHFHGHEB1BCGFFE2EGHHGHFFFHHBFFHEFCGGHHFHHHHH111BFFHGHEHBEHGGECEFHGFHFGGDBBGHHBHHHH0GH22FGHGFFFH1BFCG0F@FGG11@GDHFG1D/1FGFHH/FCG.>0><-<@CCFFG.CC-@C?C9FFF0C---;------;9----9-9@-AAFF9B9---9-/;B9BF/9BB//B/ -@M01368:8:000000000-A3GHV:1:1110:15519:10008/1 -CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAAC -+ -BBBBBFFFFFFFGGGGGGCBGGE?GGGHHHHHGHHBEGGHHGGGGGHHHHHHHHHHHGGHHHHGFGGGGHGHGCFGHHHGGGCGGHGHGGGGGHFGGGHHHHHHGCEFGGHHHHGGGGGGGHGG/CEGHHHHGFHHHGFHHGFFHGHGHFGGEGFDDGGBCGHHGFHH/CHHHHGHGHF0CFGGGGGGGFFFFFBFFFFFFGFFGGF?DGEDEEFEFFFF?FFFFFFFFFFFFFFFBFFF.BFFFBFBBA -@M01368:8:000000000-A3GHV:1:1110:16559:7507/1 -CATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAACTGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGAT -+ -ABBBAFFFFFFFFGGGGGGGFGGGGAGHHGCHHGHHHHHHHHHHHGHHGHHHHHHHHHHHHHHHHFGGGHHHHHHHHBDHHHHHGGHFEEGHGGHHHHHHHGHHHEHFGGGGGGHHHF33FGHHG3GDDGH//EEGHHFFBF?GHHFCC@DGFCGGB?DGHAFGGHHGFH0E??<@DGHGGCFGGF?DGGGFFGGGGGGGG.C/0BBF?FBBEGG0FFF.AA9;B9/E////99;;:.//:FFFFBBF// -@M01368:8:000000000-A3GHV:1:1110:16700:6843/1 -AAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAAC -+ -BBBBBFFFFFFFGGGGGGGGGGHHHHGHHHHHHHHHHHHGGGGGHHHHHGGHGHGGGGGGHHHGFFGHHGGGGHHHHHHCGG@FGHFHFEGGGGGGHGGGGDGHHHHHHHHHHHHHHGHHGHGHHGGGGGCGHEHGHHHFHHHFF.DGHHHHHHGHHHHHHGFGHGCGHGHHHHHHHHGHHHCD?:A::FCDGEGFGFFBFGBFGGGF00CBFFFF/BFBFFFFA -@M01368:8:000000000-A3GHV:1:1110:18014:7856/1 -ATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATG -+ -AABBBFFFFFFFCGGGCGGGGGGHHHHHGFHHHHHHGHHHGGGGGFHHGHFGGHHHGGFGGGHHGGGGGHHGGGGHGGHHHGGGGGGHHGHGDGGGGGGGGB/ACC=@--AFBFFB?=BFDFFF//FDB;-9A-AD-.A.A=?-9-:B/AEB/BFF.A;DDFFFBBFFFF/BBFFFFBFF9B -@M01368:8:000000000-A3GHV:1:1111:13195:14562/1 -ATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATAC -+ -ABAAAFFFFFFFGGGGGGGGGGGHHGG4FDFGFGGCGGGHGHG?EGHEGFFDHFHHHHGGFE@EHHGEEEGFCCEFFGFG/EHHHHHBFBGGHFHFHEHGHEFGGGED/EFDEFFEHHBGHHCFFFDFGGHHGFFFHHHHGFG>0<0DGGFHBGHFBDFGHGFFGGAGHHGCGBHFFHHHFFFBFGGGGF/:FFFCB9F9FF0FEFE?FF0BED-D./ -@M01368:8:000000000-A3GHV:1:1111:14287:20817/1 -GCCAACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTCATCGCACCTACGTTCACTATTACAGGCGAACATACTTATTAAAGTGTGTTAATTAAT -+ ->>A3>AAAACFFFGFGFGGGGGHGHHHHHHHGGGGGHGHFFCGHHGBGHFHHHHAGHHHHEHHAGFFEHEAE?GHHHHEGFFFDGHGHBFFHFHHHGHGHGHBECG@@@EHGGEGCGGGGFFGGGG?BGGGGGG.-C.FEDCFFFEFFFAF/99BEFA.:9BFB/9FFFEFFFFFFEFFF/FBBFFFFFFFFFFFB//;.@@//;DFFFF//BBFFFBF;A;DF=>B//;9B//B/;/;9//9//;B/:B9 -@M01368:8:000000000-A3GHV:1:1111:23624:14516/1 -GTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGGGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTA -+ -3>3AAFFFFFAFGGGGGGGGBGHHHHHHGGHGGHEHGHADFHHHHEGGGHBGHFFHHEHHGHFHGG5FBEEGEGHHHGHHHEFDGHHFFGFGGHHGHHHGHBHGHFHFHHGGGGFCFBGFGE?GFBECEGDGHEHGDGCGHGC/DF@GHGFFCGGGGFFFFDD?@C@GFEC?-ADHFFHBGHFBHFG0CC//.;B0/CA.;AA.;@.AB/9BB/A/.AFFFE/BFFF///9B/FBDE9/;99/B/BFFEF -@M01368:8:000000000-A3GHV:1:1111:7437:24508/1 -ACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGT -+ -CBCCDCFFFFFFGGGGGGGGGGGGGGHHHHHHHHHHHHHHHHGGGFFEHGGHHHHHHHHHHHGHHGGGGGHHHHHHFHHHHGHHHHGHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHGHHGGGGGGGHGGGGGCGHHHHGHHGHHHHHHHEDHHGHGGGGGD;BEGGGFFGGGGC:CFGGGGGGGGGFGGGFFFGFFFFF; -@M01368:8:000000000-A3GHV:1:1111:8665:25542/1 -TCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTGTCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGG -+ -AA3AAFFFFFFFGGGFGFFGGGGHGH4AGGGFHHCGEE2FGHHHHHHHHHHGFHGF53FHEGGFAF5CFHHGGFGHFHHGGHHHCHHHHHHHFHHGH?EEEG1FFFEHHFHFGGHHGG?F4FBGHGHCHEF0//-9--;0CFGG?@BGDGFFFFEFBBBDA?FFBFFFF=B;-9.AA9EFFFF/;9BD.//;9/9/B:/:BFBFFBFFF/B:BFF//;/B/FFFBFF.A-BFFFBA/F//BFFFBF/;/ -@M01368:8:000000000-A3GHV:1:1111:9655:13916/1 -GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGCGGTGTCCACGCAATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTT -+ -1>>1AAFFFFFBGGFE1EEEEGGHGHHH2FGHHHFGFGHGCFGGGEFHGGEGGHHHHGHEFBFEGHHHGHHBEHHHHGHGFHHHGGGGHHEGGGGHHHGFHGHHHH1FHHG?G/AGF1GBFGEFFHF2FDD0FG--99@=---AAABA-BF/B?A@F-9BBFF//BB -@M01368:8:000000000-A3GHV:1:1112:10272:7828/1 -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCAC -+ -AAABABBBBBBFGGGGGGGGGGHGHHGHGHHHHHHHHHGHHHGGHDGHHHGGGGHHHHHHHHHHHHHHHFHGHHHHGGHGGHHHFHHHHHCGHFFHHHHHHHHHHHHHGHGEFGAEEEEHHGHHGHGHHHHHHHGHHHHHHGHGHFFBC/BA?BGGGFFFFFFFFFFFFFFFC-@DDFFFFFFFEFAFFFFF -@M01368:8:000000000-A3GHV:1:1112:9832:16531/1 -CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGGGCTGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCTTGAAGTAGG -+ -AABABFFFFFFFF4GGGGEBGEFGHGGHGHGHHHGGHFBGFGGGGGHHEFHGEHFHFHEHHHHHGCGGGHFHHCFGHE@1EEEFFGB?EEEEHBGGGCHHGHHBEE@>EAHHFAEBDGGGCEFF?CEGFFHHHHHFHHHFHGHCGCAHHF<..C.D=0CD.GFHHHG.CAGHC:CHB0GGGFFB0:CFA.A-;9.;000CBBFFBFGA.9AA;A;EDDE.ABBFFFF9;BEFFF/BF//9/B//9:B -@M01368:8:000000000-A3GHV:1:1112:9832:6701/1 -CGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGG -+ -AABBBBFFFFFFGGGFGGGGGGHHHGGHGGHHHHHHHHHHHGHHGGGGGHHHHHHHHHHHHHHHHHHGGCGDHHHHFGGHHHGGGGGFGGHGGGGHHGGGGHHHHHHGGGGGGHHGGGGDGGFGHGGFCDCHHHHGHHHHGHHFAGFGGEHFGEG.C1>11F1D1DDFFFFGGG1FH3GHGHGHGHFHEC?EC0B1FBGFB1GHHH21AF2HH2FEGEHAE10AAE/>/FFHE?/E/?E1@EFFH?E@/EGGG//B11B//<@@C0F/<0AAAAFFFBFFFGGGGGGGGGGHHFHGHHHHGHHHHHHHHHHHGGGGHHHHFHHHHFHGHHHHGAFFEFHEGHHHHHHHHGHEHHGGFGGGHHHHHHFHHHHHGGHHHHGGGGGHFHHFF?HHGGFECEFFGHFFGFHGECDGHGBGFHGDF@@?CGFHCEGGGFD.CCC?EGHBHHHFHHFBCFFGEB/CEGGGGDAA.90C9CEBFGGBBF/9.9FBFFFBBFF//99FFFFEABF//99FFEFFFBFF -@M01368:8:000000000-A3GHV:1:1113:5741:16959/1 -TAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTAT -+ -ABBBBFFFFFFFGGGFGGGGGGHHHGHHGGHBGHGAGFFCAFGHGFFGHHGFHHHHHGGGGGHGHHHHHHHHE3BFFHHHGG0EDF@GHFFGGGHGGGGGGGGGGGGGHHGGEEFHGFHHDDG@DGGGHHGDGGGGGHGG?CF?HHGHHHGHGHHHFFHGGGHHHHGGCD.;0A33>FFFFFF@FFFGGGGFGGD4EGGGGGHHGFFGHFGGHHHFEFHECFHHHEHGAGBCCGCCEGGHGHEGFBFHFHHHHGGGHFHGHEGGGFEGEGG??@DBGHGGC-@FFGHFHFHB-??DA-DD@9:BEBG@B?E?A.ABBFBFA??F.FF/BFFB/B9B/9 -@M01368:8:000000000-A3GHV:1:1114:14540:5315/1 -CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGGGGGCTATTTAGGTTTT -+ -AABCCFFFFFFFGGGGGGGGGGHHHHHHHFHHHHGHHGHHGGGHGGHHHHHHHGHHHHHHGGGGGHHFHHHFGHHGGFHHHHHGGGGGHHHGHGGHHHGGGGGGHGHGGGGHHGGGGHHHHHEGDDFGFFFHHGGGGGCDAFCFGFDHHHHGGHGHHHHHHBCGEHHHHGGHG.ACGEHGG0CBFFF:A;BB0;09CGF00CFFFE0AA?//CFFFFFFFFFFFFFFFBEF;A.-=A--:BBFB90;;FE -@M01368:8:000000000-A3GHV:1:1114:15066:16302/1 -TAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAACGGTTGTTAATTAATTATT -+ -BBBBAFFBDFBFBGGGGGFGGGBGFFFHGFHHGFFFHGHHHGHHHHFFHHHGHGC?AEFFHEFBFFFGHHHHH1FDGFFHGHGHFEGCGC-<>ABFFFFFFGGCGC1BBF1GHHHBHHHHGFHGH1A100AA/GGEHGFBAA1FFD2FHHHHECG2F1BB/E0FC/A>EE/FGGFGEF1EGBBEHHCGGGHBGEHFHE0B?E--;C?CCGGG@A@GBFBBBB09?@??A-AB--ABE@=-=-9AE9A;FFFFFE=9-//;//;9FF/////;;///9B///;/B////9FFBB;--A@-;/9;9-:-/;;FFFE9BF?@;-9-99/B9F/://///99/; -@M01368:8:000000000-A3GHV:1:1114:2404:13066/1 -TCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCAT -+ -CCCCCFFFFCFFGGGGGGGGGGHHHHHGHHHHHHHHHFFHHHHHGGGGHHHHHHHHFHHHHHHFGGHHGGHGGHHHHHHGHHFHHHHGGGGGGHHHHHHGHHHHHHHHHHGGGGGGGHH?FGHHHGGGGGGHHGGFGGHHGGGGHHHHHFGGGGFGHGHHGGGGGGGHGGGEGGHHGHHHHHHHHHGFBFFDA0FGGGFFGG0:EFGGGGGGGG;AEBF0B0BFFBFFFFFFF diff --git a/tools/trim_galore/test-data/paired_collection_example_pair2_results3.fastq.gz b/tools/trim_galore/test-data/paired_collection_example_pair2_results3.fastq.gz index 176d5feaef..6f0f7d8782 100644 Binary files a/tools/trim_galore/test-data/paired_collection_example_pair2_results3.fastq.gz and b/tools/trim_galore/test-data/paired_collection_example_pair2_results3.fastq.gz differ diff --git a/tools/trim_galore/test-data/paired_collection_example_pair2_results3.fastqsanger b/tools/trim_galore/test-data/paired_collection_example_pair2_results3.fastqsanger deleted file mode 100644 index 2e31e60478..0000000000 --- a/tools/trim_galore/test-data/paired_collection_example_pair2_results3.fastqsanger +++ /dev/null @@ -1,392 +0,0 @@ -@M01368:8:000000000-A3GHV:1:1101:14518:9998/2 -CATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATA -+ -CCCCCFCCCCCFGGGGGGGGGGHHHHHHHHHHHHHHHHGFHHHHGGGGGHGFHHHHHHHHHHHHHHHHHHHGHGGEHGGGGCGGGHHGGCGGGGGHHGHHHGGGGGGGG.BFFFGAGADFGAFDGFGGCFFF;DDFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFBFFFFFFFFFFFFFFFFFFF09FFFE00;BE@;DABBFFFFFBBFB00;F:9;FFBFFF9BFFFFFFFFFFFFF90/::BFFFBF -@M01368:8:000000000-A3GHV:1:1101:18422:19051/2 -CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG -+ -BCCCCFFCFFFFGGGGGGGGGGHHHGHHHHHHHHHHHHHHHHGGGGHHHHHHHHEHHHHHHHGGHHGGHGGHHHHHHHGHGGHHHGGGGGHGHHHHGGGHFHFHHHHHGGGGGHBFFCGDHHHGGGGGGHGGGGGGHHGCGGGFGHHBGGGGGFFFHEGGGGGCDCCE@EFGHHHHFHEGHGFFHHGB;ECBFGGGEFEFFGF0AFGFGFFG.;;DFFFFFFFFFF090BFFFE?FEFBBFBFFFB990BF -@M01368:8:000000000-A3GHV:1:1101:25545:21098/2 -GCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTTCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCAACCTATGTCGCAGTATCTGTCTTTGAT -+ -3AAA34BFFFFFGCGGCGGGCGHHHGHHHHCGHFGHFHGFGGCDGGBD5FGFFFHFHGGGHAEBFGHHHEHGGFHBGGFHHHGGGEEFHHHFHFFHDHGHGHHHHFFHHGGFAEGFFFFFBGHHHFFHHHHHHHFHFHHFHGFHGHGA/<@EFHEHH1GGHFFFHG1>=FGHFHGF.GE//-AADEBGGGGFFCGGHHA5DFGAE?AAEDFHG2AEE1FBE00A1BGE0?E1FFGEEEGB4F43?EE/>///F??//@BCBAC<<.<<AABBFFCFFFGGGFGGGGGGGGHHHHHHHHHHHHHHHHHHGHHHGGEGGFFHHHHHHGHGHGHHHHHEHFHGHHHHEFHHGHGGGGGHHHHGHHHHHHHHHGFGHHHHHGHGHGHHGGGCCGGGGFGGGGGGDGGGGFGDGGGFFFFFFEFFFFFFFBAF:9AF//;BEF-DD/FFFFBFFFFFFFFFEBFF?FB;/;//BFFEFFE/::..9;-:B9:FBFFFFFF -@M01368:8:000000000-A3GHV:1:1102:17584:10050/2 -GTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGAT -+ -ABCCCFFFFFCFGGGGGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHFHGGHGGHHHHHHHHHHHHHHGHHHGGHHHHHHHGGHHHHGGGGGHHHHHHHHHHHHHHGGHHHHHHGHGHGGHGGFGGGGGGGGGGFGGFGGGGGGGGGFFFFFFFFFFEFFCFAAFEFEFFFFFFFCFDFFFFFFEFFFFFF -@M01368:8:000000000-A3GHV:1:1102:18842:24844/2 -CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG -+ -CCCCBFFBFFFFGGGGGGGGGGHHHGHHHHHHHHHHHHHHHHGGGGHHHHHHHHFHHHHHGHGHHHGGHGGHHHHGHHHHHHGHHGGGGGHFHHHFGHHHHHHHHHHHGGGGGHHHGFECGGHGGGGGGHHHGGGGHHGGGGHHHHHEFDCADGHHHHGGGGFC?AE-@DDFHFFHHHCFHGGFFGFFFGGGEFEGFGED9C?CEGF90FEF/A./BBF9FFFFFFFFBFFF.9AF////;FFBFFB/:BF -@M01368:8:000000000-A3GHV:1:1102:21788:11027/2 -AATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGC -+ -CDDDDFFFFDDFGGGGGGGGGGHHHHHGGGGHHHHHHHHGHHHHHHHGGHHGEHGGHHHHHHHHHHHHHHGGGGEHHHHHHHHHHFFFFFHHHGGGGGHFHGBFEHHHGGGGGGGHHGGGGHGGGGGHHFHHHGGG@CGHHHGGGGGGGGGGGEGDGFFDHHGEHHBCFHGBGFAGGHFGGCEEF0BE///FDFACF?FHFGC//??1B0<FF>DFCFDFFFFFFFFF-BBAFFFFFFFFE@D9B;FBFFFFF -@M01368:8:000000000-A3GHV:1:1103:25465:20010/2 -AACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGTGCACCCTATGTC -+ -AAABBFFFFFDFGGGGGGGGGGHHHHHHGHHHGFHHHHHHHGGGGGHHHHHGHHHHHHHHEHHHHHGHGGHHGGGHHHHGGGGGHHHHHEHFGEHHHEE14BGHHHGHGHFCE/EFGGGGGGHGGGGGG?FFGGGG.ADGGGFFDA.FFEC--;9BBBD?FFFFF -@M01368:8:000000000-A3GHV:1:1103:25519:6301/2 -CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATGCTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGGGATGTCTTATTT -+ ->AABBFFFFFFFFFFGGGCAFGCGHGGGHGGGHHFHHHFHHGGGGGFFFHHHBHHHHG5BG5GFEEEE1FGFFGF1EGHGGG/BFHEF@EA1BE1@EED3?4DHGGGGEGHFFFDDG?DCDEG@<?@DGCAGC-;@DGFFHGCHEEFGGFGFB/;..BBB.CCE?A0FE0EBFF0FEF.;=DFFB//BFBF//;/:9BA.9FB9//;9//;9F;FF -@M01368:8:000000000-A3GHV:1:1104:13832:4398/2 -GACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTG -+ -BBCCCFFFFFFFGGGGGGGGGGGGGHGGGGGGGHHHGFGGHHFGGGHGHHGHGFGECGHHHHGGGGGGGHEGFGGGHHGHFHHHHFGHFHHHHGHHHHFGGFGG3FFGGHHHHHHH2?FFGHFHHHHHHHHHBGHDGC0 -@M01368:8:000000000-A3GHV:1:1104:23670:4161/2 -CGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTT -+ -A3AABBFFFFFFGGGGGGGGGGHHFHHHHHHGHGHHHHHGCHHHHGGGGHHHHHHHHHHHHHHHGHHGHGHGGHGGHGHGGHHGFGHHGHHHEHHGHHHHHGHEHEHHHGGFGGHHHFHHHGHHHHHHHGHHHHG4EGGGFCBBCA?DGGGGGGCCAFFFFFFFFFFFFFFFFFDFFF.9@--=AFFFF;FBFFBEFFF9FBFFB9/FFFFFFB/FFFFFFFBFFFF/BBFBB/FFFE?.;@FFB?FB -@M01368:8:000000000-A3GHV:1:1104:24927:22253/2 -GTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGGGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGGGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAA -+ -AABBBFFFFFFFGAFGG5GGGGHDGHHHHHHHHHHHHFHCFGC2F3BFGFGHBGHHFHEDHFGGGGGHHHG3F?GHHGGC/EFEHFGAEFFHGGAEHGGGG3EEGEEDHFHAAD@DGGGHG/AC1>GED-<<-.;C0:0C:C;GH:::::0B90;FFGEGFBFF0C.;9F0;0090;900;00FA9D.A...-9=BBFFFE/FE9;9E//BBBB.BBB///B/9/B -@M01368:8:000000000-A3GHV:1:1104:4705:7727/2 -TCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCG -+ -ABBBAFFFFFFFGGFGGFGGGGGHHHHFHHHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHEDEFGHFHHHHGHGHHHHHHHGHHHHHHHHFHGHHHHHGGGGGHHHGHHHHHHHHHHHGHHHHFHGHGHGGHGGGDCD.;FFGGGGGGGGFFFFFFFFFFFFF.>FFFFFF-BBEFFFFFBFFFFF-DDFFFFFFEFFFFFFFFFFFF?F///9FFFFF/;FFFFF?AADCFFEFAFFEFFFFFB/;B/A9 -@M01368:8:000000000-A3GHV:1:1104:7756:4776/2 -CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG -+ -BBBBBFFBFFFFGGGGGGGGGFFHHGHHGHHGGHGHHGFHHGGGGGHHHHHHHHGFF3GGGHGGGHFDHEGHHFHHHHGFHBCGHGEGFFFFFHHHHHGHHHHHGHHHGGGGGHHGHHGGHHHGGGGGGHHECCFDHHADGGHHHFGFDC////FHHHDGFCGCDEECF?EGGFFHHFEHFGGHHFFF/A900;C.9..C0990.;CFBFGE/9.9B99/;B.99/FB///9AEF/99/9/BBF/B/B//A -@M01368:8:000000000-A3GHV:1:1105:11310:21761/2 -ACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCTC -+ -BCCCCFCCCCCCGGGGGGGGGGHHHHHHHHGGGGGHHHHHHHHGHHHHHHHHHGHHHHHHHHGHHHHHHHHHHGGGGGHHHHHHHHHHGHHHHHHHHHHHGGHGGHHHHGHHHDGHHHHHHHHHHHHHHHHHGHHHHHGGGGGGHHHHHGHHHHHHHHFFHHHHHCCAHFHHGGFFFFFFFFFAA;BDDFFFF00.-=D.ABDA-.::AAF;F@.AAD./0000.9-;.9:00009:F090;FF000900 -@M01368:8:000000000-A3GHV:1:1105:11578:14676/2 -GCTCCGGGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTAT -+ -CCBCCCBBCCCCGGGGGGGGGGHGGGGGHHHHHHHGHHHHGHHHHHFFFGGHGHHHHHHHHHHHHHHHHFGHHHHHHHGHHGHFHFGHHHGGGGHHHHHHHHHHHHHHFHHHHHHHGGHGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHDGGCGHHHHHHHHHHHHHFF>ABFDCFFFFFDGFGFDGDEG1EGEFFBFHFGHA0E00A0CAAC0FFDFFAGHFEHHFDH/EAFCFFHACGHHAFFEF2DFFG/FGHHHHGHGH0C@/E@GGC0FG10>GGFGHHGHFFBGFGB2>F@;-A@FF<-;---@-;-----;-BFF/;---9//BBFFFF//B;FF///9BF///;/;B/BBFBFFBB/;-9--///;9/:-;BFFBBB9F -@M01368:8:000000000-A3GHV:1:1105:15502:11453/2 -GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGAC -+ -CCCCCFFFCFFFGGGGGGGGGGHHHHHHGHHHHHGGGGGFHFHHHHHHGHHHHHGHHHHHHGHFHGHGGGGGGGGHHHGGGGGGGGHHHGHGGGGGGGGGGGHHGGGFGGGFEGGGGFFFGGFGDGGGBFGFFFFFFFFFFFFFE0BFFFFFFFFFFFFFFFFFFF;BFFDFFFFBF0BFFBFFEEBFFAA009FFFFFBFFEBB0;.BFFBFFFFFFFFFFFF0;BFFFF -@M01368:8:000000000-A3GHV:1:1105:16787:9771/2 -TCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAG -+ -CCCDCFFFFFFFGGGGGGGGGGGHHHHHHHHHHHGGGGHHHHHHHHHHHHHHFHHHHHHHGGFGGHHEHHHHHAGHHGHBHHHGHHHHHHGHGHGHHHGGGGGHHHHHHHHHHHHHHHFFHHHHHGHGHGHHGGGACAFGGGGGG-AFGGFFFFFFFFFFFFFFFFFFFFF-9@AFEDFFFFFB.9-AABBBFFFEFFFFFFFFBFFFFFFEBB0BBBFFBFFBFBEDEAFFEB.ABFBFBFFFF0:: -@M01368:8:000000000-A3GHV:1:1105:17564:15723/2 -ATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTT -+ -CCCCCCCCCFFFGGGGGGGGGGHHHHGHHGHHHHHHGHHHHHHFHHHHGGGGHHGHFHHHHHHHFHHHHHHHGHGGHGGHGHHHHFHFHHGHHHHHHHHHHHHHEHGHHHHHGGGGGHHHHGEHHGHHHHHHGHFGHFHGGAHGGHGGGCCBDFGFFAFDBDDFFFFFBDA;AAD-ADC?AFEFB-@@AAEFEFFFFF..-:.B0:FFFFFFEF0BFF09FF.0;9BFFFFF0BFFFFFF?.::-909;.9 -@M01368:8:000000000-A3GHV:1:1105:18108:12091/2 -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTA -+ -CBBCCFCCCCCFGGGGGGGGGGHHGHHHHFHHHHHHFHGHHHHFHFHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHGHHHHGHFHGHHGHHHHHHGHHHHHHGHHHHHGGGGGHHHHHHHHGHHHHHGFGHHHHHGHDHGHHGCA;?AAFGFEFDFFF.FFFFEBDFFFFFFFFBFFFFFFFFFEFEFFFEFFFFFFF.:BFFF0BFFFFFFFFFFFF;0FF0;BFFFFF -@M01368:8:000000000-A3GHV:1:1105:18331:16683/2 -TTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGGGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATAT -+ -CCCCCFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHGHHHHHFHGHHGHHHHHGGFGHHIIHHIHHHHHHHFHCFGHEEHFFHGHHFGHGHGHFHHGGGGGGEEGHHEHFHHGGHFHHFGGGGDFGBCGG?DGHGGFCGGHHECEF?CHGGDGFFGGHHC?EEGGHHHH.>AEGGGHEE-AEG0C0GCCEFFFFGEFG/;CEGGGE.:CAE.;@.@EFBFFFF/..:BFF0:BB00FBFFFFBDAFFF0; -@M01368:8:000000000-A3GHV:1:1105:20577:18421/2 -ATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGCCGAAAATACCAAATGCATGGAGAGCTCCCGTGAATGGGTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATT -+ -?ABBBFFFFFFF4CFFGGGFGGHHHHHHHCEGHHCHFGHCAFEFHHHGGAEGGHHDHHHBGHBGHHH?5BGE1AEEFHHHHHGHFHBGHGEGGGGHBFHHFGHBGCFFG4EGHGG?E/FG3F3F/FGCDECEA/FGG/CEDBB//?BHEHHF0ADC@D>D9@DDCFFFFFFCFDFE.AA;/BB/.;-.;BFBBBFFBFFFFFFF/9FF?/;/BBFFFFF/;FFB -@M01368:8:000000000-A3GHV:1:1106:17389:18671/2 -GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTCTTTAGGGTTTATGACC -+ -BBCCCFFFCBCCGGGGGGGGGGHGHGHHGFGFGFHGGHHGHHHHHHHHFHHGGGGAGFBGHHHFBBGH5GDHH5EEEGEHEGFBF1DGFDGFGGGBBCEEGEHFEFDDHFBFHFD/@BGDGDHHGDCCFCD/C<->.ADDGE1GA;9.FFFFFFD-99ADFFD-.9FADF--9..;AAFEFFEAA-;.///BB:BBF/BBB//B//99/;BBB/9////F -@M01368:8:000000000-A3GHV:1:1107:18714:24788/2 -GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACC -+ -BCCCCCFFFFFFGGGGGGGEGGHHHHGGFFHHHHHHHHHHGGGGGHHHHGHHHHHHHHHHHHHFHHHHHHHHGHHHHHHGHHHHGGCGHHHHHHHHHHHHHHHGHHHHHGDGHGGHHHHHHHHGHHHHHHHHHHHGHHHHHHEHHHGHFGGGGHHHHEHFHHGHFHHF.>GGHHHGHGHGGHCFFCDFDFFFFFFAAF.FFFFFEFAFCFFA..@.AFFFC9--9;. -@M01368:8:000000000-A3GHV:1:1107:21355:8333/2 -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCGGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTA -+ -AABCCFCCCCCFGGGGGGGGGGHHHGHHHFHHHHHHGHGHHHHHHGHHHHGGGGHHHHHHHHHFFHHFHGHHHHHHGGHGGHHHGHHHHGGHFHHHHHHHFHHGHHGEFFHFHHGGGGGGHHHFHHHHGHHHHE?FGHHHHGFGHGHHGGGDGGGGGFFFCFFFFFFFFFFFFA;DBBDFFFAFFFF;DBAFEDFFFFFEA?-9...9;9;BFFFF//BFBFFFFBFBFFFFFB/BFFF9B/EDEF-BF -@M01368:8:000000000-A3GHV:1:1107:22864:15712/2 -GTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATT -+ -AABCCFFFFFFFGGGGGGGGGGGGGHHHHHHHHHHGHHHGGGGFHGHHHHGHFGFFFFGGHGGGGGCHFHFAFEDHHGGFGGGHHEGEGGHHEGGCHGHHHHGGGGGGHGF0EECGGGGFECCFFGFBGHHHHHHGGHHGHFCFEHFBEFGGADDDC=CC0CCFHG:CEHHHGHBGH:GFFGFF0CAAA/BF09BBBBB -@M01368:8:000000000-A3GHV:1:1107:24817:14233/2 -CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAAAGTGTGGGCTATTTAGGTTT -+ -CCCCBFFFFFFFGGGGGGGGGGFHHHHHHHHHHHHHHGHHHGEHGGHHGHHHHHGHHEHHGGGGEHGHHHGEHHGHHDGHHHHGGGGGFGEFGGEGHHGCEEEGHHEFGGDHGGGGAGFHHHHGGF/?//GHFFDGGGGGHAD??DC1DHHBGHHHHGHF<FGGGGGGGGGGGFHHEFGHHHGGGGGGHHHCGGEFHFGGGFHHHHHGGGEGGHHFHEEGEG/EEEE/?/EHFHH3GGH4EGHHCB01CGHGHGD//<@CGGBFFDGHHHH0FEHHGEGFHCD11>0=GGHGCCFGHGBGGD0GGHC0C.@?ACEEC/.EDEB?BFFCFB9CFF090BBCBFBF0CCB0B00A.9E.B/9BA=-..B9FBF//;BBBBFF -@M01368:8:000000000-A3GHV:1:1108:11059:23181/2 -AACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCAC -+ -CCCCCFFFFFCCGGGGGGGGGGHHHHHHHHHHHGHHHHHHHHGHHHHHHHHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHHHHHHHFHHHHHHHHHHHHHHHHGHGHEHHGFCGGGHHHHHHHHHHHHHHHFGHHHHHGHGHGHHGGGBFGFFFFEAFFFA.BFFFEFDDFFFFFFFFFFEEFF;D;EFFFFFF0BFFFADDFFFFFBFBFFFFFBB0FFFF0::0BF000BFFFBBF0EDFF -@M01368:8:000000000-A3GHV:1:1108:12233:13498/2 -GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTA -+ -ABBBCFFFFFFFGGGGGGGGGGHHHGHHHHHHBGHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHGHHHHHGGHHGHHHHHHGHHHHHHHGGHHHHGGGGGHHHHHHHHHHHHHGHGHHHHHHGHGHGGHGGGGAGGFGGGGGGGGFFFFFFFFFFDFFFFDFFFFFFFFF>.AEDEFFFFF?E-DAB9FFFFFFFEF:FBFB009:0FFFF9BFFBFFFFBB0.9A=-/0 -@M01368:8:000000000-A3GHV:1:1108:14328:14751/2 -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTAT -+ -ABBBBFBBBBBFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHDHHHHGGGGHHHHHHHHHHHHGHHHHHHHHHGGHGGHGGHHHHHGHHHHHHHHHHHHHHHHFHGHHHHHGGGGGHHHHHGHGHHHHHHG?FHHHFHGHGHGHHGGGGGGGGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFAFFE.ADEFFFFFFFADFFFFF///BBFF/FFFFFBFAFFFFBB///;9: -@M01368:8:000000000-A3GHV:1:1108:16350:22621/2 -CATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATAT -+ -CCCCCFFFFFFFGGGGGCGGGGHHHHHHHHGHHHHGHGHHHHGGGGHHHHHHHHHHHIHHHGHHHHHHGGHGGHHHHGHGHGHHHHHHHHHHHGHHHHHHGGHHHHGGGGGHHHHHHHHHHHHHGHGHHHGHHGGGGGHHGGGDADDGFFGGGGFFFFFFFFFDFFFDFFFFFFFFFFFAFAEFEDFFFFFFEE-AAFFFFFBFFFFF0FFFBFF;AFFFFFFFFFFFFFFFFFFFFFAB9BFFFFFBFFF -@M01368:8:000000000-A3GHV:1:1108:21791:23272/2 -CATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTAT -+ -BBBBBFFFFFFFGGGGGGGGGGHHHHGGHHHHHHHGGGEGGHHFGDHGHHHHHHGGHHH2GGHHHGHHGGGGFGFBFHHHGGGGHGHGHHHHHHHHHHGHGHHGHHGGHGGHHHHGGFHGGGHFHGHHHHHGGHHHGHGFCGFFDGGG/FCFHBGHHHHHHHGHCHHHHGGGHGHGHGCFF;>@DFFBFFFFFFDFFFFFFFF;;.DDAA;FFF;BB;C@..9::BBFBBA;-:.:: -@M01368:8:000000000-A3GHV:1:1108:23314:26089/2 -CATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATT -+ -BBAAAFFFFFFFGGGGGGGGGGHHGDHHHHHFHHHHGGGGGFHFFGFFHEGHHHHHHHHFGHFHHHHDEHHHHHHGHFHHGGGGHHGFHHHHHHHHHHHHHHHHHHF?EEFCHFGFHHEFFFHGFHHGHFHFHHHFFHAG??GHACGGDGHGGBFHHHHHHHGHFGHGHGEGHGCFFF@CD-BDDFFFFFFFFDDFFBBFFD-B-A.DFFA.AFEFF-;-..:..BFFFFFA-../9FFF//B/BBBBF -@M01368:8:000000000-A3GHV:1:1108:25084:18127/2 -CCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAG -+ -CCCCCFFFFFFFGGGGGGGGGGGGHHHHHHHHFHHHHHHHHHHHHHHGGHHGHHHHHGGGGFHHHHHFHHHHHHHHHGGHHHHHCGGHGHHGGGGGGGHHGGGGGGGGHHHGHHGGGGGGGGGGGGGGGG@CDE?EFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFFFE;DFBFFFFFFFFFFFFFFFFFFFAFFFFFFFBFFE0BBBFFFFFFFFFFBFFFF9BB0:0:B; -@M01368:8:000000000-A3GHV:1:1108:7626:10048/2 -AACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGAT -+ -BCCDDFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHHHHHHHGGGGGHHHHHHHHFGHHHHHHHHHHHHGHHHHGGFHHHFGGGGHHHGHHHHHHHHHHHFGHHHHHGHGHGHHGCGCGGFHHHGGGGGGGGGGGGGGGGGGGFFFAFFFFFFAA-AFEFFFFFFFFFFFFFFFFFFFFFFFFF -@M01368:8:000000000-A3GHV:1:1109:11270:12255/2 -CTACCTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATT -+ -BCCCCFFFFFFFGGGGGGGGGGHHHHHFHHHHGGGGHHHHHHHHHHHHHHHHHHHHGHGGHGGHHGHHHHHGHHHHHHHHHFHHHHHGHHGHHHHHGGGGGHHHHHHHHGHHHHHH?FHHHHHGHGHGHHGGGGGGGGGGGGGGGGGGGGFFFFFFFFFFFFFFFFECADFFFF.DEBFFFFFF;D.B;.ED.FFFFFFF-.:00 -@M01368:8:000000000-A3GHV:1:1109:24217:19077/2 -TAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGAACCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTT -+ -BBBBBFFFFFFFGFEGGCFGFGHHHHFHHFHHHHHHGGEEEFGHGFFAGFFEEAEGGHHBEEFAFGAEF1G3GFGGEEF@EAGGHGFF?ECGGHEEEFGGHHHHFHHFHHGFHHHC2DAGHGG00-BFFA9FFFFFFFFFFFBF -@M01368:8:000000000-A3GHV:1:1109:27103:7153/2 -NTACTTCAGGGTCNTAAAACCTNAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCANGGNAGCTCTCCNNGNNNNTGGTATTNTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTAC -+ -#>>AAAFFFFFFG#AAFFGGGG#BAFFAGHHHGGGGHGHHHHFHHHHHHHGHHHHHHHEGFGGHHBGHGHHBGFFEG2GHHFHFHHHGHHGHFH#/B#??FFGHHH##?####????FHG#??CFGGGEF>CGAGAGGGFGAA@@FFFFFFFFB=@@FF?FF;FFFF@@-@-AFEF?BB/BF?E-BBFFF;:BB/BFFFB/BFFFBFBFBBFFFFFB/FFFBB/;9A@@BFFBFFFF/BBFFFF -@M01368:8:000000000-A3GHV:1:1109:8763:7859/2 -GGTTCCTACTTCAGGGTCATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAG -+ ->AAABFFFFFFFGGGGGGGGGGHHHHHHHCHFAFHHDGEGFHHHHHHHHFHHHHFHHHHHHGHGGHGGHHHFHGHHHHHFFHHHHHHBGHFFHGHGHHHHHGGEGGHHHHHGHHHHHHHHHGFGHHHHGHGHGHHCCGGGGGGGGGGAAGF?FFFFBFA@=BDDFF?-;FFF;@CFFEFEFFFFFFFFFBFDFFFFFFFFFFFFFFFFFFFFFF:FFFF/BF/FFFEFF?DECAFEFFFFFE:FFFFFFFB -@M01368:8:000000000-A3GHV:1:1110:12808:18367/2 -GCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACAGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATT -+ -ABCCCCDDDFFFGGGGGGGGGGHHHHHHHHHGGGGGGHHHHFHHGHHHHHHHHHHHHHGHHHGGHHHH3GFHEFHHHHHGHHGFHHHHHFGHHHHHGHGHGGHGGGGGGGHHHGGCGGGGHHGGGGGGGGGGGGGGGGGGGF-BAEFEBEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFFAFEFDFFFFFFFFFF0BFBED=@DFFFFFFFFFEFFFFFBFAFB;0: -@M01368:8:000000000-A3GHV:1:1110:14751:26540/2 -ATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGGTTGGGCTATTTGGGTTTTTTGACC -+ -BBBBBFBBBBBFGGGGGGGGGGHHHHGGFHGGHGGCHHHHHHGHHHGHHGEGGGHHHHHHHHHHEFHHHHHHGEE?FGHGEHGGHHHGGGGGGEHHGGGGHHGGGEGHHHGHGGGDFGHHGHGGGGGCGAFFCCDFHHHH1CGGHBHFHFCGFFCDFHGCCE.CC:EGFHHCGHHGCF?FGGGFGGG;B9;FFFFFFEGEBF0;;;0;CFBFFF?D.DE.:..99-...;;//9A.;DE.9-./: -@M01368:8:000000000-A3GHV:1:1110:15519:10008/2 -GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACA -+ -BBCBCFFFFFFFGGGGGGGGGGHHHGHHHFHHBGHHHGGGGHHHHHHGHHHHHHHHHHHHHHHGGHGGHHGHHHGGBGHHHHHHHHHHHHHHHHHGGFHHHGGGEGHHHHHHHHHHHHHHHGHHHHHHGHGHGHHGGGGGGDGGGGGGGGGBFFFFFFFFFFFFFFFFFFFECB-=BFFFFFFFFBB?E-A::FFBFFFFFBFFFFFFFFFFFFBFFEFFF;0BFF0:FE?FFFFBFFFFFFFFFFFFFF -@M01368:8:000000000-A3GHV:1:1110:16559:7507/2 -GCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAAT -+ ->AABBCCCCFFFGGGGGGGGGGHHHHHHHHHGGHGGHGHFHHHHFHHHHHHHHHHFHHGHHHHGHHHHHGGEEGHHHHHHHHHHHHHHHHHHHHHHGHGHGHHGGGGGGGHHGGGFGAGFGGGGGGGGGGGDDDFAGGFFFFCFAFEEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFFFFFFFFFFFFFFE..@BFFBD./;:0FFFFF09B/AFCA=-99BFFBFFFFFFFB:BFBFFBFB9: -@M01368:8:000000000-A3GHV:1:1110:16700:6843/2 -GTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTT -+ -CBCCDFFFFFFFGGGGGGGGGGHHGHHHGGHFHHHGGGGGHHHHHHHHHHGHHHHHHHHHHHGGHGGHHHHHHHGBGFFHHHHHHGHHHHHHHHGHHHHHGGGGGHHHHHHHHHHHHHHHFHHHHGHCG/GGHHGGGGGGAFGEEADAGGDFFFF00D>CFDDFFFB?AF?B;-@>.FEDFFFFF0BF-BDFFFFE00B0BF0FFFF:BFA0B0:BBFBBFFFFF -@M01368:8:000000000-A3GHV:1:1110:18014:7856/2 -CATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTAT -+ -CDDDBFCDDDDFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHGGGGGHHHHHHHHHGHHHHHEGHHHHHEHGHGHHGGGCCGGHGHGCGGGGDHHHHFHGGGGGGGGGGGFGGFGAC-EFEGGGFFFGFF;DFFFBFFFFFFFFFFFFFFFFFFFEFFFBFFFFFFFFF -@M01368:8:000000000-A3GHV:1:1110:23303:11846/2 -GTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTAAGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTA -+ -BBCBBFFFFFCFGGGGGGGGGGHHHHHHGGHHHHHHHHGH5GHHHGGGGHHHHHFHHHHHHHEHHGFBGEEFE1FG5GFHHHHHFHHHGGGGGHHHHHHHHHHHGGHHHHHGGGGFHHFHH/EHHHGG?@EFHHHGGGGHFGGGGHHFHGGDGGGGFFFHGFDGGGCGACC;DFFB0CH0CCCFGBFBFB/:A?FFFE?AA.CFBECE0BBBF0F/A./9FFFFF./;/FFFFFFA.:////9BB/B/BB -@M01368:8:000000000-A3GHV:1:1111:13195:14562/2 -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGAT -+ -ABBBBFBBBBBFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHDGHHHGGGGHHHHHHHHHHHHGHHHHHHHHHGGGGGHHGHHHHGFGFHHHHHHHHGHHHHHHHGHHHHHGGGGGHHHHGHHHHHHHHHHGFHHHHHGFGHGHHGGGGFCGFGFFFFAFFFFFFFFFFA;BADFFFAFFFFFFFFFEFFFEFFFFFFF;FDBFFFFFFFFFFFF -@M01368:8:000000000-A3GHV:1:1111:14287:20817/2 -ATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATGAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGTTAGACCTGTGATC -+ -CCCCBFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHHHHHHHHHGHHHGHHHHGHHHGHGHFHHHGGGGHHHHHHHHBGGGFHHFGGHHGGHGHHHHHHHHEGFFHHFGGGGGHHEHHHBHHHHHFFHHGHGGGGGHFGF3FDGHHGCGGFGHHGGGG/2GG@E?GHHHGFEDGGCFDFFFFFFFFFFFBFFFFFFFFFFF/FFF/BFFFFFFFBA.?@;FFBDFFFF -@M01368:8:000000000-A3GHV:1:1111:7437:24508/2 -ACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGT -+ -BBBBBFFBABBBGGGGGGGGFGHHHHHHHHHHHHHGHHHGFHHHHGGGGDHHHHHHHHGFHHHHHGHHHHHHGHFHGHHGGGDFGDFFGGGFFGFFGHGHHHGGC?AD-:A-AFFGGG-?9EFEFGGFFBFEFCFFFFFFFBBBFFFFFFFFFFFFFFFBBFFFFFFFFFFFFFFFFFFEFFFFFFBFFFFEFEFFFF;CF-FFFFFFFF/BFFFFFFF -@M01368:8:000000000-A3GHV:1:1111:8665:25542/2 -CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGACAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTT -+ -CCCCCFFFFFFFGGGGGGGGGGHHHHHHHFHHHHCHHGDGGGGHGGHHHHHHHHGHGHHHGGGGGHFHHHHFHHHFFHHHHHFGCGGEHFHHFGGHFHGGGGGGHHFEEEAFEFFDFHGHHHHGGGGGGGHH0DGGGGCGHCC-FFFFFFFGGGGGGGGGGHHHHHGHHHHGHHGGHHGGHGGHHHGHHHHFHHGHHAFGGGHHHHHGHHHHFHFHHHHHECGGGHGHHHEFHFHGGGGGGGGFGFFGFFEFGEGHHHGHHGGDGGGHGFGGGGGGGGE@GCGGHHHGHHHHHGHHHHFHHGEGFFEGCE.ECCECEHCGGFFE/AABFFG0CFE0BFFF9CFGG.F/BBBFFGFGGGFFFFFFBFEDAEFFFFFBFFFFFFF0;FEBF: -@M01368:8:000000000-A3GHV:1:1112:10272:7828/2 -GTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATAC -+ -BBCCBFFCCCCCGGGGGGGGGGGGGGHHHHHHGGGGGGHHFHGGGGGFGHGGGGGGHHHHHHHHHHFHHHHFHGFFHHGF?ECEFFFFFFHHHHHH?FHHHHHHHHHGFHHHHHHGGGFGHHHHFHHHHHHHHHHGGGFGFGCGGGGHHHHHHHHHHHGGFFHGHHHHHFFHFCGFHCCGHGHCGGHG?DGG -@M01368:8:000000000-A3GHV:1:1112:9832:16531/2 -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTA -+ -BBBBBFBBBBBFGGGGGGGGGGHFHHHHHHHHHHHHHHGHHHHGHFHHHGEEGGHHHGHHHHHHHHHHHHEHGHHGGGHGGHGHHHHHGHHHHGHHHHHHHHHHHHHHGHHHHHGEGCGHHHHHGHHHHHHHHHHHHHHGHFFGHGHHGGGDACDFFFFABFFFFFFFFFFD-BC?DA9.DFFBEA==B=?F.;ABBFBFEF-DAF/BFF9/FFFBB/9BFFFFFFF/BFBBFFFFFFF/9FEA?--B/ -@M01368:8:000000000-A3GHV:1:1112:9832:6701/2 -GCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATT -+ -BBBBAFFFFFFFGGGGGGGGGGFGHHHHHGHHHHHHHHHHHHCGHHHHHHHGHHHHHHDGHHGGAECHHGHHHHHHHGHHHGHGFHHHHGGHGGHHFHHHHHHFHHHHHHHHH2GHHHHGHGHHFHHGGFGGGHHGHHHHHFHHHHEFHHHHEFGFFHGEHCFGDFDFFFFFCFFFFFFFFFFFFFAFBFFDFFFFFFFFADCF?9?EFBFB9EF-A.;BFFFFFFFBFFFFFFFFFFFFBFFFFFFFFFF -@M01368:8:000000000-A3GHV:1:1113:13532:8618/2 -GCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTGGAT -+ -CDDDBFFFFFFFGGGGGGGGGGHHHHHGGHHHGHHHGHHGGGGEHHGGHHHHHHHHHHHHHHGHHGHHHHHGGHHHHHFHHHHGCDCGHHHHHHHHEHHHGHGHHHGFHGGHGGHHHHHHHHFHHHFHHHGHHHHHHHHHFGGHHHHGGGEGAFHHGHHGGHHHHGHFBGFHGHGHGHGGHGGF@DAFEFBECAB=A.0BFFFEDF=A9.@D.-AAFFFA-:-..:.9/0:;0..-..0000::BFB09:: -@M01368:8:000000000-A3GHV:1:1113:21679:18011/2 -TTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATC -+ -BCCCBFFFFFFFGGGGGGGGGGHHHHHFHHHGHHHGFGHGGHGGHHHHHHGHHHHHHHGGGGFHHHHHHEGHHHHHHHHFGGFCGEHFHFGEGHHHFGGGGGHHHGGGEHGGGGGHHHHHHGDCCCGHHHHDGGGGCD/FCC->DFF -@M01368:8:000000000-A3GHV:1:1113:5741:16959/2 -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTA -+ -BBBBCFCCCCCFGGGGGGGGGGHHHHHHHGHHHHHHHHGHHHHGHDGHHHGGGGHHHHHHHHHHHHHHHGHHHHHGGGEGGHHHHFHHHHHHHHHHHHHHHHHHHHHHGHHGHHGGGGGHHHHHHHHHHHGHHHFHHHHHHGHGHGHGGGGCGGFGGFFFFFFFFFFFFFBFFFFFFFFFFFFFFAFFFFEAEFFFFFFFFFFF9BFFBFFFFFFFFFFFFFFBFFFFFFFFFFFFFFFFFFADAB-/B -@M01368:8:000000000-A3GHV:1:1114:10130:11959/2 -ATCAGAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACG -+ -BCCDDFFFFFFFGGGGGGGGGGGHHHHHGGHHHGGGCGGHGHGGGGHHGGGGHHHHHHGGGEGGHHHFGGGGG?E1FE?/EEHHHHHGHHGHHHHGHFHGHGHHGDGGFG2FF2?GHHHHHGCCCFHGHGHHHHGHHFEHHFGHHGHH<1=DGHHHGHHGHGAGAE -@M01368:8:000000000-A3GHV:1:1114:14540:5315/2 -CACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGC -+ -CCCCCFFCCDCCGGGGGGGGGGHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHHGHHHHHHHGHHHHFHFHHHGGGGGHHHHHHHHHHHHHGHHHHHHHHGGGGGGHHHFHHHGHHHHHHHHHHHHHHHHHHGHGHHHGGGFGHHHHHHHHFHHHHHF?1FHHGHGHGHGHHGGFFFFDBFBE;BCC.:BFFFFFFFFFFFFFF;AFFFFF-=-.AEDEFFFFF..9A;9FFFF0FFFFE00FFF0:B -@M01368:8:000000000-A3GHV:1:1114:15066:16302/2 -TTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAG -+ -CCCCCFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHHHDGHHHHFHHGHHGHHHHHGGGGEHHHHFHHFF5FHHFEGHHHGDHGGHGHGFGGGEHFGHHGGGGGHGHHHGHHFHHB3FGHHFGGGG?GFFHCCEBGFFECCDFEGFCFGCHHGFDDHHHGHHCFGGGGGFBFDGFG?-:..AFG.-C0C009;:00;00:9/:CEFFF?AE::9;9?0:FEF0; -@M01368:8:000000000-A3GHV:1:1114:16639:15258/2 -TTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCGCACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGAT -+ -CDCCCFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHGHHHHHHGGGGGHGIHHHIH5DEGHHHF?FGHGGHGGHEGGHFHHGHGEHHGGGGGFFFGHFBG2GHEBGHHGHGGEG/GFGABEDFGHEED?GGHHFFGGGCFEGD/GFHFFGEFGCGG?CC??D-EF@EEEFGCDDBBFGGGEBBFFF09090A.BFGA.9CCA0;EBAB00BBFF.@-./;BB;BFFF0:00099AAFFF -@M01368:8:000000000-A3GHV:1:1114:2404:13066/2 -ATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGACCATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGA -+ -CCCCCFFFFFFCGGGGGGGGGGHHHHHHHHHFFHHHHGGGGGHFFFHHFHHHHHHHHHHHHHHHGFEGGGHGEDFCDFHGHFG@@DGGHHHHHHGGGGCGGGGGEHGGCGBB?CF99EGFGGFGG?D9CFFFF/BBFFFFFEF9BFFAFFFFEFFFFFFFFFFFFFFFFFFFFF.FFBBFFFFFFFFFFFF-9;;;BFFFFFB9BFBFBFABFFEFFFFFFFFFF::BFFBFF diff --git a/tools/trim_galore/test-data/paired_collection_example_results3.txt b/tools/trim_galore/test-data/paired_collection_example_results3.txt index c910fdb35a..b30f76de20 100644 --- a/tools/trim_galore/test-data/paired_collection_example_results3.txt +++ b/tools/trim_galore/test-data/paired_collection_example_results3.txt @@ -1,52 +1,38 @@ - SUMMARISING RUN PARAMETERS -========================== +========================= Input filename: input_1.fastq Trimming mode: paired-end -Trim Galore version: 0.6.10 -Cutadapt version: 5.0 -Python version: 3.12.10 -Number of cores used for trimming: 4 +Trim Galore version: 2.2.0 (Oxidized Edition) Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 -Using Nextera adapter for trimming (count: 29). Second best hit was Illumina (count: 0) -Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) -Maximum trimming error rate: 0.1 (default) +Adapter sequence: 'CTGTCTCTTATA' (user-specified or auto-detected) +Maximum trimming error rate: 0.1 Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp -Length cut-off for read 1: 35 bp (default) -Length cut-off for read 2: 35 bp (default) -This is cutadapt 5.0 with Python 3.12.10 -Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq -Processing single-end reads on 4 cores ... +Trim Galore 2.2.0 (Oxidized Edition) — adapter trimming built in +This is cutadapt 4.0 (compatible; for MultiQC backwards compatibility) +Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq +Processing reads on 1 core in single-end mode ... === Summary === -Total reads processed: 99 -Reads with adapters: 52 (52.5%) -Reads written (passing filters): 99 (100.0%) +Total reads processed: 99 +Reads with adapters: 52 (52.5%) +Reads written (passing filters): 99 (100.0%) -Total basepairs processed: 24,849 bp -Quality-trimmed: 205 bp (0.8%) -Total written (filtered): 23,339 bp (93.9%) +Total basepairs processed: 24,849 bp +Quality-trimmed: 205 bp (0.8%) +Total written (filtered): 23,339 bp (93.9%) === Adapter 1 === -Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times +Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times. -Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-12 bp: 1 -Bases preceding removed adapters: - A: 9.6% - C: 38.5% - G: 23.1% - T: 28.8% - none/other: 0.0% - Overview of removed sequences length count expect max.err error counts 1 11 24.8 0 11 @@ -57,80 +43,70 @@ length count expect max.err error counts 13 2 0.0 1 2 14 1 0.0 1 1 16 1 0.0 1 1 -17 1 0.0 1 0 1 -20 2 0.0 1 2 -21 1 0.0 1 1 -24 1 0.0 1 1 -26 2 0.0 1 2 -31 1 0.0 1 1 -33 1 0.0 1 1 -41 2 0.0 1 2 -49 1 0.0 1 1 -50 1 0.0 1 1 -54 1 0.0 1 1 -56 1 0.0 1 1 -58 2 0.0 1 2 -60 1 0.0 1 1 -67 2 0.0 1 2 -68 1 0.0 1 1 -69 1 0.0 1 1 -73 1 0.0 1 1 -80 1 0.0 1 1 -86 1 0.0 1 1 +17 1 0.0 1 1 +20 2 0.0 2 2 +21 1 0.0 2 1 +24 1 0.0 2 1 +26 2 0.0 2 2 +31 1 0.0 3 1 +33 1 0.0 3 1 +41 2 0.0 4 2 +49 1 0.0 4 1 +50 1 0.0 5 1 +54 1 0.0 5 1 +56 1 0.0 5 1 +58 2 0.0 5 2 +60 1 0.0 6 1 +67 2 0.0 6 2 +68 1 0.0 6 1 +69 1 0.0 6 1 +73 1 0.0 7 1 +80 1 0.0 8 1 +86 1 0.0 8 1 + RUN STATISTICS FOR INPUT FILE: input_1.fastq ============================================= 99 sequences processed in total - +Sequences removed because they became shorter than the length cutoff of 20 bp: 1 (1.0%) +Sequences removed because they were longer than the upper length cutoff: 0 (0.0%) +Sequences removed because of too many N bases: 0 (0.0%) SUMMARISING RUN PARAMETERS -========================== +========================= Input filename: input_2.fastq Trimming mode: paired-end -Trim Galore version: 0.6.10 -Cutadapt version: 5.0 -Python version: 3.12.10 -Number of cores used for trimming: 4 +Trim Galore version: 2.2.0 (Oxidized Edition) Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 -Using Nextera adapter for trimming (count: 29). Second best hit was Illumina (count: 0) -Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) -Maximum trimming error rate: 0.1 (default) +Adapter sequence: 'CTGTCTCTTATA' (user-specified or auto-detected) +Maximum trimming error rate: 0.1 Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp -Length cut-off for read 1: 35 bp (default) -Length cut-off for read 2: 35 bp (default) -This is cutadapt 5.0 with Python 3.12.10 -Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq -Processing single-end reads on 4 cores ... +Trim Galore 2.2.0 (Oxidized Edition) — adapter trimming built in +This is cutadapt 4.0 (compatible; for MultiQC backwards compatibility) +Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq +Processing reads on 1 core in single-end mode ... === Summary === -Total reads processed: 99 -Reads with adapters: 58 (58.6%) -Reads written (passing filters): 99 (100.0%) +Total reads processed: 99 +Reads with adapters: 58 (58.6%) +Reads written (passing filters): 99 (100.0%) -Total basepairs processed: 24,849 bp -Quality-trimmed: 745 bp (3.0%) -Total written (filtered): 23,035 bp (92.7%) +Total basepairs processed: 24,849 bp +Quality-trimmed: 745 bp (3.0%) +Total written (filtered): 23,035 bp (92.7%) === Adapter 1 === -Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times +Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times. -Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-12 bp: 1 -Bases preceding removed adapters: - A: 12.1% - C: 37.9% - G: 8.6% - T: 41.4% - none/other: 0.0% - Overview of removed sequences length count expect max.err error counts 1 16 24.8 0 16 @@ -146,29 +122,42 @@ length count expect max.err error counts 16 1 0.0 1 1 17 1 0.0 1 1 19 2 0.0 1 2 -21 1 0.0 1 1 -25 1 0.0 1 1 -30 1 0.0 1 1 -32 2 0.0 1 2 -34 1 0.0 1 1 -36 2 0.0 1 2 -38 1 0.0 1 1 -40 1 0.0 1 1 -41 1 0.0 1 1 -42 1 0.0 1 1 -43 1 0.0 1 1 -49 1 0.0 1 1 -51 1 0.0 1 1 -56 1 0.0 1 1 -57 1 0.0 1 1 -60 1 0.0 1 1 -67 1 0.0 1 1 -80 1 0.0 1 1 +21 1 0.0 2 1 +25 1 0.0 2 1 +30 1 0.0 3 1 +32 2 0.0 3 2 +34 1 0.0 3 1 +36 2 0.0 3 2 +38 1 0.0 3 1 +40 1 0.0 4 1 +41 1 0.0 4 1 +42 1 0.0 4 1 +43 1 0.0 4 1 +49 1 0.0 4 1 +51 1 0.0 5 1 +56 1 0.0 5 1 +57 1 0.0 5 1 +60 1 0.0 6 1 +67 1 0.0 6 1 +80 1 0.0 8 1 + RUN STATISTICS FOR INPUT FILE: input_2.fastq ============================================= 99 sequences processed in total +Sequences removed because they became shorter than the length cutoff of 20 bp: 1 (1.0%) +Sequences removed because they were longer than the upper length cutoff: 0 (0.0%) +Sequences removed because of too many N bases: 0 (0.0%) + +=== Paired-end validation === + +Number of sequence pairs analysed: 99 + +Number of sequence pairs removed because at least one read was shorter than the length cutoff (1.01%): + 1 +Number of pairs removed for N content (0.00%): + 0 -Total number of sequences analysed for the sequence pair length validation: 99 +Unpaired read 1 kept: 1 +Unpaired read 2 kept: 0 -Number of sequence pairs removed because at least one read was shorter than the length cutoff (20 bp): 1 (1.01%) diff --git a/tools/trim_galore/test-data/paired_collection_example_results3gz.txt b/tools/trim_galore/test-data/paired_collection_example_results3gz.txt index 3cd6bee72d..78e51ce226 100644 --- a/tools/trim_galore/test-data/paired_collection_example_results3gz.txt +++ b/tools/trim_galore/test-data/paired_collection_example_results3gz.txt @@ -1,53 +1,39 @@ - SUMMARISING RUN PARAMETERS -========================== +========================= Input filename: input_1.fastq.gz Trimming mode: paired-end -Trim Galore version: 0.6.10 -Cutadapt version: 5.0 -Python version: 3.12.10 -Number of cores used for trimming: 4 +Trim Galore version: 2.2.0 (Oxidized Edition) Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 -Using Nextera adapter for trimming (count: 29). Second best hit was smallRNA (count: 0) -Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) -Maximum trimming error rate: 0.1 (default) +Adapter sequence: 'CTGTCTCTTATA' (user-specified or auto-detected) +Maximum trimming error rate: 0.1 Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp -Length cut-off for read 1: 35 bp (default) -Length cut-off for read 2: 35 bp (default) Output file will be GZIP compressed -This is cutadapt 5.0 with Python 3.12.10 -Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq.gz -Processing single-end reads on 4 cores ... +Trim Galore 2.2.0 (Oxidized Edition) — adapter trimming built in +This is cutadapt 4.0 (compatible; for MultiQC backwards compatibility) +Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq.gz +Processing reads on 1 core in single-end mode ... === Summary === -Total reads processed: 99 -Reads with adapters: 52 (52.5%) -Reads written (passing filters): 99 (100.0%) +Total reads processed: 99 +Reads with adapters: 52 (52.5%) +Reads written (passing filters): 99 (100.0%) -Total basepairs processed: 24,849 bp -Quality-trimmed: 205 bp (0.8%) -Total written (filtered): 23,339 bp (93.9%) +Total basepairs processed: 24,849 bp +Quality-trimmed: 205 bp (0.8%) +Total written (filtered): 23,339 bp (93.9%) === Adapter 1 === -Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times +Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times. -Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-12 bp: 1 -Bases preceding removed adapters: - A: 9.6% - C: 38.5% - G: 23.1% - T: 28.8% - none/other: 0.0% - Overview of removed sequences length count expect max.err error counts 1 11 24.8 0 11 @@ -58,81 +44,71 @@ length count expect max.err error counts 13 2 0.0 1 2 14 1 0.0 1 1 16 1 0.0 1 1 -17 1 0.0 1 0 1 -20 2 0.0 1 2 -21 1 0.0 1 1 -24 1 0.0 1 1 -26 2 0.0 1 2 -31 1 0.0 1 1 -33 1 0.0 1 1 -41 2 0.0 1 2 -49 1 0.0 1 1 -50 1 0.0 1 1 -54 1 0.0 1 1 -56 1 0.0 1 1 -58 2 0.0 1 2 -60 1 0.0 1 1 -67 2 0.0 1 2 -68 1 0.0 1 1 -69 1 0.0 1 1 -73 1 0.0 1 1 -80 1 0.0 1 1 -86 1 0.0 1 1 +17 1 0.0 1 1 +20 2 0.0 2 2 +21 1 0.0 2 1 +24 1 0.0 2 1 +26 2 0.0 2 2 +31 1 0.0 3 1 +33 1 0.0 3 1 +41 2 0.0 4 2 +49 1 0.0 4 1 +50 1 0.0 5 1 +54 1 0.0 5 1 +56 1 0.0 5 1 +58 2 0.0 5 2 +60 1 0.0 6 1 +67 2 0.0 6 2 +68 1 0.0 6 1 +69 1 0.0 6 1 +73 1 0.0 7 1 +80 1 0.0 8 1 +86 1 0.0 8 1 + RUN STATISTICS FOR INPUT FILE: input_1.fastq.gz ============================================= 99 sequences processed in total - +Sequences removed because they became shorter than the length cutoff of 20 bp: 1 (1.0%) +Sequences removed because they were longer than the upper length cutoff: 0 (0.0%) +Sequences removed because of too many N bases: 0 (0.0%) SUMMARISING RUN PARAMETERS -========================== +========================= Input filename: input_2.fastq.gz Trimming mode: paired-end -Trim Galore version: 0.6.10 -Cutadapt version: 5.0 -Python version: 3.12.10 -Number of cores used for trimming: 4 +Trim Galore version: 2.2.0 (Oxidized Edition) Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 -Using Nextera adapter for trimming (count: 29). Second best hit was smallRNA (count: 0) -Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) -Maximum trimming error rate: 0.1 (default) +Adapter sequence: 'CTGTCTCTTATA' (user-specified or auto-detected) +Maximum trimming error rate: 0.1 Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp -Length cut-off for read 1: 35 bp (default) -Length cut-off for read 2: 35 bp (default) Output file will be GZIP compressed -This is cutadapt 5.0 with Python 3.12.10 -Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq.gz -Processing single-end reads on 4 cores ... +Trim Galore 2.2.0 (Oxidized Edition) — adapter trimming built in +This is cutadapt 4.0 (compatible; for MultiQC backwards compatibility) +Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq.gz +Processing reads on 1 core in single-end mode ... === Summary === -Total reads processed: 99 -Reads with adapters: 58 (58.6%) -Reads written (passing filters): 99 (100.0%) +Total reads processed: 99 +Reads with adapters: 58 (58.6%) +Reads written (passing filters): 99 (100.0%) -Total basepairs processed: 24,849 bp -Quality-trimmed: 745 bp (3.0%) -Total written (filtered): 23,035 bp (92.7%) +Total basepairs processed: 24,849 bp +Quality-trimmed: 745 bp (3.0%) +Total written (filtered): 23,035 bp (92.7%) === Adapter 1 === -Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times +Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times. -Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-12 bp: 1 -Bases preceding removed adapters: - A: 12.1% - C: 37.9% - G: 8.6% - T: 41.4% - none/other: 0.0% - Overview of removed sequences length count expect max.err error counts 1 16 24.8 0 16 @@ -148,29 +124,42 @@ length count expect max.err error counts 16 1 0.0 1 1 17 1 0.0 1 1 19 2 0.0 1 2 -21 1 0.0 1 1 -25 1 0.0 1 1 -30 1 0.0 1 1 -32 2 0.0 1 2 -34 1 0.0 1 1 -36 2 0.0 1 2 -38 1 0.0 1 1 -40 1 0.0 1 1 -41 1 0.0 1 1 -42 1 0.0 1 1 -43 1 0.0 1 1 -49 1 0.0 1 1 -51 1 0.0 1 1 -56 1 0.0 1 1 -57 1 0.0 1 1 -60 1 0.0 1 1 -67 1 0.0 1 1 -80 1 0.0 1 1 +21 1 0.0 2 1 +25 1 0.0 2 1 +30 1 0.0 3 1 +32 2 0.0 3 2 +34 1 0.0 3 1 +36 2 0.0 3 2 +38 1 0.0 3 1 +40 1 0.0 4 1 +41 1 0.0 4 1 +42 1 0.0 4 1 +43 1 0.0 4 1 +49 1 0.0 4 1 +51 1 0.0 5 1 +56 1 0.0 5 1 +57 1 0.0 5 1 +60 1 0.0 6 1 +67 1 0.0 6 1 +80 1 0.0 8 1 + RUN STATISTICS FOR INPUT FILE: input_2.fastq.gz ============================================= 99 sequences processed in total +Sequences removed because they became shorter than the length cutoff of 20 bp: 1 (1.0%) +Sequences removed because they were longer than the upper length cutoff: 0 (0.0%) +Sequences removed because of too many N bases: 0 (0.0%) + +=== Paired-end validation === + +Number of sequence pairs analysed: 99 + +Number of sequence pairs removed because at least one read was shorter than the length cutoff (1.01%): + 1 +Number of pairs removed for N content (0.00%): + 0 -Total number of sequences analysed for the sequence pair length validation: 99 +Unpaired read 1 kept: 1 +Unpaired read 2 kept: 0 -Number of sequence pairs removed because at least one read was shorter than the length cutoff (20 bp): 1 (1.01%) diff --git a/tools/trim_galore/test-data/paired_collection_example_unpair1_results3.fastq.gz b/tools/trim_galore/test-data/paired_collection_example_unpair1_results3.fastq.gz index 34596d67c5..0a38706076 100644 Binary files a/tools/trim_galore/test-data/paired_collection_example_unpair1_results3.fastq.gz and b/tools/trim_galore/test-data/paired_collection_example_unpair1_results3.fastq.gz differ diff --git a/tools/trim_galore/test-data/paired_collection_example_unpair1_results3.fastqsanger b/tools/trim_galore/test-data/paired_collection_example_unpair1_results3.fastqsanger deleted file mode 100644 index a8fa963bf3..0000000000 --- a/tools/trim_galore/test-data/paired_collection_example_unpair1_results3.fastqsanger +++ /dev/null @@ -1,4 +0,0 @@ -@M01368:8:000000000-A3GHV:1:1101:6911:8255/1 -ATCTGGTTCCTACTTCAGGGCCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATT -+ -BCCCCFFFFFFFGGGGGGGGGGGHHHHGHGHHHHHHHHHGGGGGGHHHHGHHHHHHHHHHGHHHHHHGGHGGHHHGHHHHFHHGHHHHHHHHHGHEHEFFGHHEGGCEFGGFHHHBGHHGHHHHGHFHHHGHGHGHGGCDFDDACGGGGGGGAAFFFFFFFFFBAFFFFFB;FFFFFFADDFFFFFFFFFFEFFFFFFFFFFBFFFFFFFFFFFFFFEFFFFFFFFBFEFFFFEFE;DFFFDFBFF/9BFB diff --git a/tools/trim_galore/test-data/paired_collection_example_unpair2_results3.fastq.gz b/tools/trim_galore/test-data/paired_collection_example_unpair2_results3.fastq.gz index 898d76873b..f9631332bb 100644 Binary files a/tools/trim_galore/test-data/paired_collection_example_unpair2_results3.fastq.gz and b/tools/trim_galore/test-data/paired_collection_example_unpair2_results3.fastq.gz differ diff --git a/tools/trim_galore/test-data/paired_collection_example_unpair2_results3.fastqsanger b/tools/trim_galore/test-data/paired_collection_example_unpair2_results3.fastqsanger deleted file mode 100644 index e69de29bb2..0000000000 diff --git a/tools/trim_galore/test-data/paired_example_pair1_results2.fastq.gz b/tools/trim_galore/test-data/paired_example_pair1_results2.fastq.gz index 953dc1fce8..ff0dc41d36 100644 Binary files a/tools/trim_galore/test-data/paired_example_pair1_results2.fastq.gz and b/tools/trim_galore/test-data/paired_example_pair1_results2.fastq.gz differ diff --git a/tools/trim_galore/test-data/paired_example_pair1_results2.fastqsanger b/tools/trim_galore/test-data/paired_example_pair1_results2.fastqsanger deleted file mode 100644 index 0d55c14fd4..0000000000 --- a/tools/trim_galore/test-data/paired_example_pair1_results2.fastqsanger +++ /dev/null @@ -1,392 +0,0 @@ -@M01368:8:000000000-A3GHV:1:1101:14518:9998/1 -GTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTG -+ -AAAAAFFFFFFFGGGGGGGGGGHGGHHHHGHHHHHHHGCGHHHHHHHHHHHHHHHGGGGGHHHHHHHHHGHHGFHFE5BGEEHFGGGHHHHHHHHFBHHGGGGFHGHHFGHHHHGHHHHHHGEGGGGFHFHGEGHHGGCDGDGHGGGDGGHGGCGGGHGHHH/ACDG?.1FGCDCCGCA.CC@CDCHFHGFFGGGEBFGAB//CEFBFGG.:;D;;A0AFFFFFB..:@ABFF//;BFFFFFBF/9D:A -@M01368:8:000000000-A3GHV:1:1101:18422:19051/1 -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTA -+ -CCCCCFDDDDDFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHFHHHHGGGGHHHHHHHHHGHHHHHHHHHHHHGGHGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHGCGGGHHHHHHHHHHHHHHHHHHHHHHGFDHGFHCFGGGGFGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF;FFFFFFFFFFFFFFFFFFFFFFFFFFFFEFBFFFFFFFFFF:FF -@M01368:8:000000000-A3GHV:1:1101:25545:21098/1 -ATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATAAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGGCTTATTTAAGGGGAACGGGTGG -+ -33AA?DFD5BDFGGGFEBDGEGHEGHGEGHCEGGHHCHGHHFFHHGFGAGE53FF2FAFFGDE5FFFE5GFBFGAEE1GHHHGHHHEHE3FGHF@GEGEGGHHGG3FAGFFDE?EEE3GFEGFGFGGCG?GHHHFHGGGC@DHFFHD/AA@AAAAD2ADDFFGGGGGFGGHA?EAEFBEAGHFABFGG5FDF5DB1EEGAFDFB53FF5FH@G5FFEHGHEFHFFHBE333GF43GCGGGGE@0?BFGGB0B?FHGFDGGHHHBFFDEGGHGFFFDFE@<1>@FFFGHHHHFHEFGDABFFGG/@DCE;>=EF?AEFFFF;9;=DAFFFFBBFFFBFFF//;BFB.:FBFFBB/BFFFF/BFFE?EFAFEF9 -@M01368:8:000000000-A3GHV:1:1104:11928:24197/1 -ACGTAGGTGCGATAAATAATGGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATA -+ -CDDCCFCFFFBCGGGGGGGGGGHHHGHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHGGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHGHHHHGHHGHHHHGGHGEEGGGGFHHFHHHGHGEHHHHHHHHHHHHHHHHHGGGGGGFGGGGGGGGFFGGGFGFGAEFEFGFEAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF@D9 -@M01368:8:000000000-A3GHV:1:1104:12288:20635/1 -CACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGCCGCAGCATCTGTCTTTGATTCCTGC -+ -ABA?AFBAB2>AFGGGGFFGD5GGHGFFHGHEGGGGHHHHFFHH5FHGHHHFFHEFHHHHHHGGHHHHHGFHGGHGGGGFHGGGFBHHHFGEGHHHHGHGGGGECEHHFHHGHHFFHFGGGHHHEGHDGHHGHGHFHHHECGCBCHHGFHHFHD1GFHEFHEF?GDCFFEFE0A@;@>BD/9BFBBBBFDFFFF99D=?>DDBFF-AFFEBB9BBEFEFAFFF//-@---;;FBBFBFB///;99999B -@M01368:8:000000000-A3GHV:1:1104:13832:4398/1 -CACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTC -+ -AAABABCAFFFFGGGGGGGGGGHFHHHHHHHHHGFFHHGGHGGGGGHHHHHHHHHHFHHHHGHHHHHGGHGHGGHGGGGGGGHGFFGGGGDGGHHHHGGGGFGGGGGCFGGEGCDGGGGGGGGGGGGEGF?;BFFFFFF -@M01368:8:000000000-A3GHV:1:1104:23670:4161/1 -ATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATC -+ -AAAABFFFFFFFGGGGGGGCGBBGGHHHHHHHHHGHHHHHHHHHHFGGHHHHHHGGFHHHHHHHGGGGGFHHHGHHHHHGHHHHGHHGGGHGGGGHHHHHGHHHHHHEGGGFHHHGFFDGEGHGHFHGHHGEGGGHHBGHGGHGHGGGGG/@FCDFDGFHGGGDFFHFGFFGGGGGHHHHGGGGGGGEGCDG?GGBFFFEE00BFFGABBBAFGGGEGEA.9FFF/./;BFBF./AAF/BFFBBF/F/BF -@M01368:8:000000000-A3GHV:1:1104:24927:22253/1 -AGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTAT -+ -AAA?AFFF3DFFFGGBGFF3EAEEEHFHHFHHHGHHHHDGHHGAEFGHHHGFCHHGHHHBGHHHAEGGHGGFHFBFHHHHHHHHHHFHH1EEHEEHH1FFDEEFCGHBGHHFHEHHHHHH1FCGHHHHEEFGEEGFFCHHH11>GHHF/FG2DGGCGAGGHGGGG@@@BEFFF?@@@FFEFFFFFF?@@FB@BB@FFFE=;==---A9EFBBB/;E;A9B/B/FFFEF/B///;/;9/A/;/9BFFFBFBB -@M01368:8:000000000-A3GHV:1:1104:4705:7727/1 -CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG -+ -BBBBBFFBFFFFGGGGGGGGGGHHHGHHHGHHHHH6GHHHHFFGGGHHHHHHHHHHHHHHHGGFGGGEGGGHHHHHHHHHHGHE3FGGGGHHHHHFHHHHHHHHHHHHGGGGGHHFHFCGFHHGGGGGGGHHGGGGHHGGGGGGHHHHGGGADCHHHHGDGGGGGGGGC@DGFHEHHHHHHGHHHHGFGDEGFGFFGGGGCFGBEGFGGGFG;C.BFFFFFFFBFBFFFF/F?.AFFF/BFFEFFFFFEFF -@M01368:8:000000000-A3GHV:1:1104:7756:4776/1 -GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTG -+ -CCCCCCFFFFFFGGGGGGGEEFHHHHGHGHHHHHHGGHHHGGGGGGHHGHHHHHGHHGHHHGGFHHHHHHHHGHHHFHHGHHGFGGGGHHHHHHHHGHGHFFHFHHHHHHGGHGFGFHHHGHG?FGHHHHHHHHHHHHHHGHGHHGHGGCDGFFHHHHGHHHHHGHHHGFHGHFHGHGHGH/@CABFFFFFFFACAFF?BBFFBFD-@-.D>FFAF.F.:9---;AADFFFFFBEFFF:9FFFFBBFFFF -@M01368:8:000000000-A3GHV:1:1105:11310:21761/1 -ATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTT -+ -CCCCBFFFFFFFGGGGGGGGGGGHHHHHHHHHHHHGHHHHHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHGHHHGHHHGHGHHGHHHHGGHGGG=FFGEHHHHHGHFGGHHGHHHHHFHHHHHHHGGHHHHHHHGHEHHGHHHHHGGGGF9AGE?DEGFFGGGGGGGGGGGGGGFFFF/9FFBFFFFFFFEFFFBFFFFFFFFFFFFEFFF -@M01368:8:000000000-A3GHV:1:1105:11578:14676/1 -ATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGT -+ -CDDDDFFFBFFCGGGGGGGGGGHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHFGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHHHHHHHHHGHHHHGGGGGGHGGHGHHHHHHH;CHHHHHHHHHHHHHHHGHGGGGFGGGFGGGGGGGGGGGGFGGGEGGFFFFFFFFFFFFFFFFFFFFFFFFF/FFFFFFFFFFFFFFFF -@M01368:8:000000000-A3GHV:1:1105:13575:19255/1 -TTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGTTCCCGTGAGTGGTTAATAGGGTGATAGGCCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG -+ -AA1A1DFFFFFBGG3A111FFAEGDDBGFDHF00F01BBA00BFGEHFBFFA1FABB0FAE?CFHF1BGFHFB0011F121E//>>1F10>B?EFHGCC/?/BFG/EEGFFEE//BCFDE0?E@@B/?11AAFFFFFEAGGGFGGAA6FHDGGCGFHFFHFHFHHHHHGDDDFFFHGFFHGBEGEGHHHFFFHGEEAEGHHHHHFHHHFHGHFEDGGEGBEAGEFHHFFHHHHHHGGHHHGHHGBGHHFHHFGHHHHG?CG?AFHH1FHHHGHFHHHF?DGHFFHCGAGEFHGCBBBB?BFFF?B-B.9/BFFFFFB=-AD?..@FFFF@@-@@?AFD.FFFBBEEBD9/:FFFFFBB/:FF9BF/BF.BBFF/ -@M01368:8:000000000-A3GHV:1:1105:21099:2895/1 -TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATT -+ -CBCBBFFFFFFFGGGGGGGGGGGGGFHHHHFHGHHHHHHFHHHHFGFHHHHHHGHHHHHHGHHEHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHHHHHHHFHHHHGHGHHHGGHHHHHGGHFFGHGCGGGHGHHGHHHFGHHFHH/FGGGHFGFEGGCFCCD;B@DFFFFFFFFFFFFFFFFFFFFFFFFF;F.BEFADFFFF.AEFFFFFFE;D.;:B00099:09BFBFFBFF.0:000BFFBF -@M01368:8:000000000-A3GHV:1:1105:26469:19245/1 -TTATGATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCAAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATG -+ -BAAAAFFFBFFFGGGGGGFFGGHHHHHHHHHHHHGGHHGHHHGGHHHHHHHHHHHDEGGHGHHHHHGEDHFHGFHGGHHGGHGGEHHHGHHGHHGHHHGGEGGGHGHGHHHHHHGHHHHGHGGGGGHFHGGGGHHGGGGCDGHHHGD>A@FFFFFFFGGGFEECFGGFFB5GBHH5DFH5GBEEAEFHFHHF3F3GHFHHHEF3GAEHBGFHFFEFFHHHHFFB31FGGEGFHGEHHHHHHHHHHHHHHHHHEEEEGHHHFFF3GF3FGF44BBGEHHHHGG2B/BGGHE;B=.9.;.BBFFF..;A;/B/;B//;;//BBF -@M01368:8:000000000-A3GHV:1:1106:22927:3863/1 -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGACTT -+ -A>ABAFA?D@AFGGGGFGGGFGFHHHFFH4BCBGHHHHGFFF3G5BF3322EEAFHHHHHHAB5GHFHEHFHHH3BEEHFGF5@13FDF3GDGFHHFGHHHGHHHGFGGHHFCG//?/EGHHHG3F4BFFGHHHEDGFHHB/B/?FBF<@-9?BDEFFF?B--?DFFFB9FA>B;A.BFDB?AB.--@;-.FFAEFFFF9AE;DDFFFFB/9BF -@M01368:8:000000000-A3GHV:1:1106:23625:4236/1 -TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATT -+ -@?AAAFFBFBFFFGGFGFGGGEFCGGHHHHFGHGHFHGHHHHGGFGGFGHHGHGHHHHGHGFFHHGGGGGHHHHHHHHHBFHHEFHHHHHHGF?FAGHGFHHHGDGFHHGGGHGHFHHHGHHHGHHFHHGFFGFHHHGHHHHFFHHHHH/FGHHHHGHGHGBFFF9 -@M01368:8:000000000-A3GHV:1:1107:14451:26748/1 -GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGAC -+ -BABBBFFDABBBGGGGGGGGGGHGHHHHGAGHGGGGGHHHHHHHHGHHHHHGGGGGGFFHHHHHHHHHFHHHHHGGGGCHFHH3FEHHGCGGGDGHHHGFGGHHGGGGHHHHHHG/DGGGHHHHGGGGGCCHGG?CGG1GHHGHHHHBGHHHFHHGHHHHGGADAD00ECEGHEFHHFFAEFFFFFGGFBFBFFFGFFG.;B/99;BCFGGGGEFFFFFFEDA./:ADEB?FBFFFF;FFEBFBFF -@M01368:8:000000000-A3GHV:1:1107:18714:24788/1 -GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACC -+ -BBBCCFFFDEDEGGGGGGGGGGHHHHHHGGHHGGHGGHHHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHCFGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHGHHHHHHHHHHHGHHHHGGEGEGCCEGGHHHHHHG:CAFFGGGGGGBFEFGGGGHGAFFGGFGGGGGGGEFFFFFFFFFEF/EDDFFFFFFFFFBFFFFFBBFF -@M01368:8:000000000-A3GHV:1:1107:21355:8333/1 -ATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATG -+ -ABCBBFFFFFAFGGGGGGGGGGHHHHHHHHFHHHHHGGGGGHHHHHHFHHHGHHHHHHHGGGGGHHHHFFGHGHGGGGGGGHGGEGGGHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHGHHHFHHHHHHHHGHFFHHHHGGHGEGHHGHGFHHFHGHCEFHEGHGHHHGHHHGGGGGGCFFFFBFGGFGGGGFBG?FDGGGEGFFFFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFF.AA/9 -@M01368:8:000000000-A3GHV:1:1107:22864:15712/1 -AATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTAC -+ -BBBBBFBBFFFFGGGGCEGGGGHGHHDGFHHHHHHHHHHHHHCHHGHFGBGDFGE?GHHGHFBHHFHHHHHHHHHGHFGFGHGHHGGC@DCFGHHGGGGGGGHHHHHHGGGGGFGGGGFFGGGGFADGFEFFFFFFFFFFF9;FFFF/BFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFEAECDFFFFFFFFFBFFFFF -@M01368:8:000000000-A3GHV:1:1107:24817:14233/1 -CTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGA -+ -3AABABFFBFFFGCFGGGGGGGHHHFGCFGGGFFGHHCGHHHGHGHGHBFFHHHEDGGFGFGHHFFFHGGEGHFHHGHFHGFFHHHFGHHHFHACECEHHHBGFHGFHHHFHHF4GFHFHFHEHGGE/EG:A-CFGGFGADGGGGFGGGGDAAAADFA.-AFFECFDAAFFFFEFFFFBFF;D;BFFFB/;BFFFFFFFFFFFFFFBFFBF/;//;BFFFF?FFFFFFEDAFFFFFBF//:BFBACF -@M01368:8:000000000-A3GHV:1:1107:24830:14440/1 -AACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCA -+ -ABBBA@FFFFBBGGGGGGGGGGHGHHGHHGHHHGGHHHHHGHGHHGDHHHHHHAGGGGFHHGHHHHHHGHHHHHHHHHHHEGAFGFHFHHHHGEGHHHHHHHHHHHHHHHHHGGHHHGEFEGGHHGHHHHHGGHGFHH/?GHHHHGFGHGFHCGGBBDAFFFEFFFFFFFFFFFFFF=CDDADDFFFFEF=BFFFEFFFBFFFFEEBF./:FFFFFFFFFFFFFFFFFFFEFFFFFFFFFFF/BFBEFF -@M01368:8:000000000-A3GHV:1:1107:26478:23602/1 -GCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGAT -+ -1AAAAF1B13BBBEGGGFGGGGGGHHDB1BGFHHHFGFGGG0F00BGBGFGGF1GBGB1BGCGAEE/FGFHGHFHHHHHBFF1B0FGGF//F//FGFGFFF/1@B1DFHHHGGEFEDHFDFAFF0B//1FDD1GFFHHDF?1BD>GF/GCAC0C@?@-;99BBB->@--;-B//99B--9-BF=A--A-:A----9AA9AFBFBBB?E@BBFFFFFFFBFFFFF -@M01368:8:000000000-A3GHV:1:1108:11059:23181/1 -GTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGA -+ -CCCCCFFFFFFFGGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHGHHGGGGHIIIHHHHHHHGHHHHGHHHGGHGGHHHHHHHHHHHHHHGGGGGHHGHHHHHHHHHHHHHHHGGGGGHHHHHFGHHHGGGGGGHHHGGGGGHGGGGHHHHHHGGGGGGHGGHEFGGGGGHGGG-ECHHHGGGGGGGGGFGFFGGGGFGGGCF?EFGG0;BFFFFB?9AAFFFFFFBF;BBFFBFFFFE?F -@M01368:8:000000000-A3GHV:1:1108:12233:13498/1 -TAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACC -+ -BCCBAFFBCCCCGGGGGGGGGGHHHHGGGGGHHHHHHGHGHBGFHHHHHGGGGGHHGGBFGHHGGGGGGGHHHGGGGHHGGGFHHHHHHGGGGGGHGHHGGGGGGGGGGGGGGHHHHFHHHGHGHHHHHHGHGHHGGFGGGDFFGGHHHGHGH/FHHHFHGHGHHGHHHGHHBAAAA3BDFFFFDGBFFEEGGGGHHGHFHHCFHGHGHHHGF3BFGFHGFEFHFHGH3EGFC1EEGBFGBHFGGFFHHHGHFHGHHGHGGHCEHEBCFF3ED3@GH4FDEG12FFHFFHG/G3FGA/@EFEFCG33FFGBBGHFHH/?BFBGFGFAGC2C/@-;@-;BFFEFADF?DFFFFFF?;AFFA;AD;..FEFFA--;9;D.9BFFBE?;.;///://///99//BB/9;F./B//BBFB9// -@M01368:8:000000000-A3GHV:1:1109:25237:11722/1 -CCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATG -+ -AABBBFFFFFBBF5BGGFGABGHGGAEFGFHHHHFHCHFHHBFGGGEHBGGHFFFFGFAEF5FHH?EGGF5AEG3?EBFHD?C/EFHFFEGEE?CFGGGHHFHB3EEFG?EEGHCFGGGGGGGECA?FFFDFBFGCG4EGFG??GACFGGHFHCFHCHBECDEFCEGHHHHHFFGGF3AFFHHFGEGCGHHGEHGFGHHEGG?EFHHH?EGGHHGGGGHHHHHHEGGGGGHFHHGFDDGGACFCC@?/FDDGHHHGHEFFHHH0FDHHFFEDCD.CFHHGDFGGGGGEFGC?EGHHHB0CGHHGCGHHHHBEHGHH.::ACA;FECFGGGGGFFFEFGGGGGGGFFBBBFGBFFFEFFFFBFFFFFFBBFBFFB9..AF?FFFFF -@M01368:8:000000000-A3GHV:1:1110:14751:26540/1 -TAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACTGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGTTGTGCACGCGATAGCATTGCGCGACGCTGGCGCCGGCGCACCCTATGTCGCAGTATCTGTCTTTGATT -+ -AAA?1CFFFFADGEEGFGB1112DGDGHGFGHHCG/ECEBGGHDFGHHFHGHEB1BCGFFE2EGHHGHFFFHHBFFHEFCGGHHFHHHHH111BFFHGHEHBEHGGECEFHGFHFGGDBBGHHBHHHH0GH22FGHGFFFH1BFCG0F@FGG11@GDHFG1D/1FGFHH/FCG.>0><-<@CCFFG.CC-@C?C9FFF0C---;------;9----9-9@-AAFF9B9---9-/;B9BF/9BB//B/ -@M01368:8:000000000-A3GHV:1:1110:15519:10008/1 -CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAAC -+ -BBBBBFFFFFFFGGGGGGCBGGE?GGGHHHHHGHHBEGGHHGGGGGHHHHHHHHHHHGGHHHHGFGGGGHGHGCFGHHHGGGCGGHGHGGGGGHFGGGHHHHHHGCEFGGHHHHGGGGGGGHGG/CEGHHHHGFHHHGFHHGFFHGHGHFGGEGFDDGGBCGHHGFHH/CHHHHGHGHF0CFGGGGGGGFFFFFBFFFFFFGFFGGF?DGEDEEFEFFFF?FFFFFFFFFFFFFFFBFFF.BFFFBFBBA -@M01368:8:000000000-A3GHV:1:1110:16559:7507/1 -CATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAACTGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGAT -+ -ABBBAFFFFFFFFGGGGGGGFGGGGAGHHGCHHGHHHHHHHHHHHGHHGHHHHHHHHHHHHHHHHFGGGHHHHHHHHBDHHHHHGGHFEEGHGGHHHHHHHGHHHEHFGGGGGGHHHF33FGHHG3GDDGH//EEGHHFFBF?GHHFCC@DGFCGGB?DGHAFGGHHGFH0E??<@DGHGGCFGGF?DGGGFFGGGGGGGG.C/0BBF?FBBEGG0FFF.AA9;B9/E////99;;:.//:FFFFBBF// -@M01368:8:000000000-A3GHV:1:1110:16700:6843/1 -AAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAAC -+ -BBBBBFFFFFFFGGGGGGGGGGHHHHGHHHHHHHHHHHHGGGGGHHHHHGGHGHGGGGGGHHHGFFGHHGGGGHHHHHHCGG@FGHFHFEGGGGGGHGGGGDGHHHHHHHHHHHHHHGHHGHGHHGGGGGCGHEHGHHHFHHHFF.DGHHHHHHGHHHHHHGFGHGCGHGHHHHHHHHGHHHCD?:A::FCDGEGFGFFBFGBFGGGF00CBFFFF/BFBFFFFA -@M01368:8:000000000-A3GHV:1:1110:18014:7856/1 -ATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATG -+ -AABBBFFFFFFFCGGGCGGGGGGHHHHHGFHHHHHHGHHHGGGGGFHHGHFGGHHHGGFGGGHHGGGGGHHGGGGHGGHHHGGGGGGHHGHGDGGGGGGGGB/ACC=@--AFBFFB?=BFDFFF//FDB;-9A-AD-.A.A=?-9-:B/AEB/BFF.A;DDFFFBBFFFF/BBFFFFBFF9B -@M01368:8:000000000-A3GHV:1:1111:13195:14562/1 -ATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATAC -+ -ABAAAFFFFFFFGGGGGGGGGGGHHGG4FDFGFGGCGGGHGHG?EGHEGFFDHFHHHHGGFE@EHHGEEEGFCCEFFGFG/EHHHHHBFBGGHFHFHEHGHEFGGGED/EFDEFFEHHBGHHCFFFDFGGHHGFFFHHHHGFG>0<0DGGFHBGHFBDFGHGFFGGAGHHGCGBHFFHHHFFFBFGGGGF/:FFFCB9F9FF0FEFE?FF0BED-D./ -@M01368:8:000000000-A3GHV:1:1111:14287:20817/1 -GCCAACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTCATCGCACCTACGTTCACTATTACAGGCGAACATACTTATTAAAGTGTGTTAATTAAT -+ ->>A3>AAAACFFFGFGFGGGGGHGHHHHHHHGGGGGHGHFFCGHHGBGHFHHHHAGHHHHEHHAGFFEHEAE?GHHHHEGFFFDGHGHBFFHFHHHGHGHGHBECG@@@EHGGEGCGGGGFFGGGG?BGGGGGG.-C.FEDCFFFEFFFAF/99BEFA.:9BFB/9FFFEFFFFFFEFFF/FBBFFFFFFFFFFFB//;.@@//;DFFFF//BBFFFBF;A;DF=>B//;9B//B/;/;9//9//;B/:B9 -@M01368:8:000000000-A3GHV:1:1111:23624:14516/1 -GTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGGGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTA -+ -3>3AAFFFFFAFGGGGGGGGBGHHHHHHGGHGGHEHGHADFHHHHEGGGHBGHFFHHEHHGHFHGG5FBEEGEGHHHGHHHEFDGHHFFGFGGHHGHHHGHBHGHFHFHHGGGGFCFBGFGE?GFBECEGDGHEHGDGCGHGC/DF@GHGFFCGGGGFFFFDD?@C@GFEC?-ADHFFHBGHFBHFG0CC//.;B0/CA.;AA.;@.AB/9BB/A/.AFFFE/BFFF///9B/FBDE9/;99/B/BFFEF -@M01368:8:000000000-A3GHV:1:1111:7437:24508/1 -ACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGT -+ -CBCCDCFFFFFFGGGGGGGGGGGGGGHHHHHHHHHHHHHHHHGGGFFEHGGHHHHHHHHHHHGHHGGGGGHHHHHHFHHHHGHHHHGHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHGHHGGGGGGGHGGGGGCGHHHHGHHGHHHHHHHEDHHGHGGGGGD;BEGGGFFGGGGC:CFGGGGGGGGGFGGGFFFGFFFFF; -@M01368:8:000000000-A3GHV:1:1111:8665:25542/1 -TCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTGTCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGG -+ -AA3AAFFFFFFFGGGFGFFGGGGHGH4AGGGFHHCGEE2FGHHHHHHHHHHGFHGF53FHEGGFAF5CFHHGGFGHFHHGGHHHCHHHHHHHFHHGH?EEEG1FFFEHHFHFGGHHGG?F4FBGHGHCHEF0//-9--;0CFGG?@BGDGFFFFEFBBBDA?FFBFFFF=B;-9.AA9EFFFF/;9BD.//;9/9/B:/:BFBFFBFFF/B:BFF//;/B/FFFBFF.A-BFFFBA/F//BFFFBF/;/ -@M01368:8:000000000-A3GHV:1:1111:9655:13916/1 -GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGCGGTGTCCACGCAATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTT -+ -1>>1AAFFFFFBGGFE1EEEEGGHGHHH2FGHHHFGFGHGCFGGGEFHGGEGGHHHHGHEFBFEGHHHGHHBEHHHHGHGFHHHGGGGHHEGGGGHHHGFHGHHHH1FHHG?G/AGF1GBFGEFFHF2FDD0FG--99@=---AAABA-BF/B?A@F-9BBFF//BB -@M01368:8:000000000-A3GHV:1:1112:10272:7828/1 -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCAC -+ -AAABABBBBBBFGGGGGGGGGGHGHHGHGHHHHHHHHHGHHHGGHDGHHHGGGGHHHHHHHHHHHHHHHFHGHHHHGGHGGHHHFHHHHHCGHFFHHHHHHHHHHHHHGHGEFGAEEEEHHGHHGHGHHHHHHHGHHHHHHGHGHFFBC/BA?BGGGFFFFFFFFFFFFFFFC-@DDFFFFFFFEFAFFFFF -@M01368:8:000000000-A3GHV:1:1112:9832:16531/1 -CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGGGCTGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCTTGAAGTAGG -+ -AABABFFFFFFFF4GGGGEBGEFGHGGHGHGHHHGGHFBGFGGGGGHHEFHGEHFHFHEHHHHHGCGGGHFHHCFGHE@1EEEFFGB?EEEEHBGGGCHHGHHBEE@>EAHHFAEBDGGGCEFF?CEGFFHHHHHFHHHFHGHCGCAHHF<..C.D=0CD.GFHHHG.CAGHC:CHB0GGGFFB0:CFA.A-;9.;000CBBFFBFGA.9AA;A;EDDE.ABBFFFF9;BEFFF/BF//9/B//9:B -@M01368:8:000000000-A3GHV:1:1112:9832:6701/1 -CGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGG -+ -AABBBBFFFFFFGGGFGGGGGGHHHGGHGGHHHHHHHHHHHGHHGGGGGHHHHHHHHHHHHHHHHHHGGCGDHHHHFGGHHHGGGGGFGGHGGGGHHGGGGHHHHHHGGGGGGHHGGGGDGGFGHGGFCDCHHHHGHHHHGHHFAGFGGEHFGEG.C1>11F1D1DDFFFFGGG1FH3GHGHGHGHFHEC?EC0B1FBGFB1GHHH21AF2HH2FEGEHAE10AAE/>/FFHE?/E/?E1@EFFH?E@/EGGG//B11B//<@@C0F/<0AAAAFFFBFFFGGGGGGGGGGHHFHGHHHHGHHHHHHHHHHHGGGGHHHHFHHHHFHGHHHHGAFFEFHEGHHHHHHHHGHEHHGGFGGGHHHHHHFHHHHHGGHHHHGGGGGHFHHFF?HHGGFECEFFGHFFGFHGECDGHGBGFHGDF@@?CGFHCEGGGFD.CCC?EGHBHHHFHHFBCFFGEB/CEGGGGDAA.90C9CEBFGGBBF/9.9FBFFFBBFF//99FFFFEABF//99FFEFFFBFF -@M01368:8:000000000-A3GHV:1:1113:5741:16959/1 -TAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTAT -+ -ABBBBFFFFFFFGGGFGGGGGGHHHGHHGGHBGHGAGFFCAFGHGFFGHHGFHHHHHGGGGGHGHHHHHHHHE3BFFHHHGG0EDF@GHFFGGGHGGGGGGGGGGGGGHHGGEEFHGFHHDDG@DGGGHHGDGGGGGHGG?CF?HHGHHHGHGHHHFFHGGGHHHHGGCD.;0A33>FFFFFF@FFFGGGGFGGD4EGGGGGHHGFFGHFGGHHHFEFHECFHHHEHGAGBCCGCCEGGHGHEGFBFHFHHHHGGGHFHGHEGGGFEGEGG??@DBGHGGC-@FFGHFHFHB-??DA-DD@9:BEBG@B?E?A.ABBFBFA??F.FF/BFFB/B9B/9 -@M01368:8:000000000-A3GHV:1:1114:14540:5315/1 -CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGGGGGCTATTTAGGTTTT -+ -AABCCFFFFFFFGGGGGGGGGGHHHHHHHFHHHHGHHGHHGGGHGGHHHHHHHGHHHHHHGGGGGHHFHHHFGHHGGFHHHHHGGGGGHHHGHGGHHHGGGGGGHGHGGGGHHGGGGHHHHHEGDDFGFFFHHGGGGGCDAFCFGFDHHHHGGHGHHHHHHBCGEHHHHGGHG.ACGEHGG0CBFFF:A;BB0;09CGF00CFFFE0AA?//CFFFFFFFFFFFFFFFBEF;A.-=A--:BBFB90;;FE -@M01368:8:000000000-A3GHV:1:1114:15066:16302/1 -TAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAACGGTTGTTAATTAATTATT -+ -BBBBAFFBDFBFBGGGGGFGGGBGFFFHGFHHGFFFHGHHHGHHHHFFHHHGHGC?AEFFHEFBFFFGHHHHH1FDGFFHGHGHFEGCGC-<>ABFFFFFFGGCGC1BBF1GHHHBHHHHGFHGH1A100AA/GGEHGFBAA1FFD2FHHHHECG2F1BB/E0FC/A>EE/FGGFGEF1EGBBEHHCGGGHBGEHFHE0B?E--;C?CCGGG@A@GBFBBBB09?@??A-AB--ABE@=-=-9AE9A;FFFFFE=9-//;//;9FF/////;;///9B///;/B////9FFBB;--A@-;/9;9-:-/;;FFFE9BF?@;-9-99/B9F/://///99/; -@M01368:8:000000000-A3GHV:1:1114:2404:13066/1 -TCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCAT -+ -CCCCCFFFFCFFGGGGGGGGGGHHHHHGHHHHHHHHHFFHHHHHGGGGHHHHHHHHFHHHHHHFGGHHGGHGGHHHHHHGHHFHHHHGGGGGGHHHHHHGHHHHHHHHHHGGGGGGGHH?FGHHHGGGGGGHHGGFGGHHGGGGHHHHHFGGGGFGHGHHGGGGGGGHGGGEGGHHGHHHHHHHHHGFBFFDA0FGGGFFGG0:EFGGGGGGGG;AEBF0B0BFFBFFFFFFF diff --git a/tools/trim_galore/test-data/paired_example_pair2_results2.fastq.gz b/tools/trim_galore/test-data/paired_example_pair2_results2.fastq.gz index 321f0c6d55..6f0f7d8782 100644 Binary files a/tools/trim_galore/test-data/paired_example_pair2_results2.fastq.gz and b/tools/trim_galore/test-data/paired_example_pair2_results2.fastq.gz differ diff --git a/tools/trim_galore/test-data/paired_example_pair2_results2.fastqsanger b/tools/trim_galore/test-data/paired_example_pair2_results2.fastqsanger deleted file mode 100644 index 2e31e60478..0000000000 --- a/tools/trim_galore/test-data/paired_example_pair2_results2.fastqsanger +++ /dev/null @@ -1,392 +0,0 @@ -@M01368:8:000000000-A3GHV:1:1101:14518:9998/2 -CATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATA -+ -CCCCCFCCCCCFGGGGGGGGGGHHHHHHHHHHHHHHHHGFHHHHGGGGGHGFHHHHHHHHHHHHHHHHHHHGHGGEHGGGGCGGGHHGGCGGGGGHHGHHHGGGGGGGG.BFFFGAGADFGAFDGFGGCFFF;DDFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFBFFFFFFFFFFFFFFFFFFF09FFFE00;BE@;DABBFFFFFBBFB00;F:9;FFBFFF9BFFFFFFFFFFFFF90/::BFFFBF -@M01368:8:000000000-A3GHV:1:1101:18422:19051/2 -CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG -+ -BCCCCFFCFFFFGGGGGGGGGGHHHGHHHHHHHHHHHHHHHHGGGGHHHHHHHHEHHHHHHHGGHHGGHGGHHHHHHHGHGGHHHGGGGGHGHHHHGGGHFHFHHHHHGGGGGHBFFCGDHHHGGGGGGHGGGGGGHHGCGGGFGHHBGGGGGFFFHEGGGGGCDCCE@EFGHHHHFHEGHGFFHHGB;ECBFGGGEFEFFGF0AFGFGFFG.;;DFFFFFFFFFF090BFFFE?FEFBBFBFFFB990BF -@M01368:8:000000000-A3GHV:1:1101:25545:21098/2 -GCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTTCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCAACCTATGTCGCAGTATCTGTCTTTGAT -+ -3AAA34BFFFFFGCGGCGGGCGHHHGHHHHCGHFGHFHGFGGCDGGBD5FGFFFHFHGGGHAEBFGHHHEHGGFHBGGFHHHGGGEEFHHHFHFFHDHGHGHHHHFFHHGGFAEGFFFFFBGHHHFFHHHHHHHFHFHHFHGFHGHGA/<@EFHEHH1GGHFFFHG1>=FGHFHGF.GE//-AADEBGGGGFFCGGHHA5DFGAE?AAEDFHG2AEE1FBE00A1BGE0?E1FFGEEEGB4F43?EE/>///F??//@BCBAC<<.<<AABBFFCFFFGGGFGGGGGGGGHHHHHHHHHHHHHHHHHHGHHHGGEGGFFHHHHHHGHGHGHHHHHEHFHGHHHHEFHHGHGGGGGHHHHGHHHHHHHHHGFGHHHHHGHGHGHHGGGCCGGGGFGGGGGGDGGGGFGDGGGFFFFFFEFFFFFFFBAF:9AF//;BEF-DD/FFFFBFFFFFFFFFEBFF?FB;/;//BFFEFFE/::..9;-:B9:FBFFFFFF -@M01368:8:000000000-A3GHV:1:1102:17584:10050/2 -GTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGAT -+ -ABCCCFFFFFCFGGGGGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHFHGGHGGHHHHHHHHHHHHHHGHHHGGHHHHHHHGGHHHHGGGGGHHHHHHHHHHHHHHGGHHHHHHGHGHGGHGGFGGGGGGGGGGFGGFGGGGGGGGGFFFFFFFFFFEFFCFAAFEFEFFFFFFFCFDFFFFFFEFFFFFF -@M01368:8:000000000-A3GHV:1:1102:18842:24844/2 -CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG -+ -CCCCBFFBFFFFGGGGGGGGGGHHHGHHHHHHHHHHHHHHHHGGGGHHHHHHHHFHHHHHGHGHHHGGHGGHHHHGHHHHHHGHHGGGGGHFHHHFGHHHHHHHHHHHGGGGGHHHGFECGGHGGGGGGHHHGGGGHHGGGGHHHHHEFDCADGHHHHGGGGFC?AE-@DDFHFFHHHCFHGGFFGFFFGGGEFEGFGED9C?CEGF90FEF/A./BBF9FFFFFFFFBFFF.9AF////;FFBFFB/:BF -@M01368:8:000000000-A3GHV:1:1102:21788:11027/2 -AATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGC -+ -CDDDDFFFFDDFGGGGGGGGGGHHHHHGGGGHHHHHHHHGHHHHHHHGGHHGEHGGHHHHHHHHHHHHHHGGGGEHHHHHHHHHHFFFFFHHHGGGGGHFHGBFEHHHGGGGGGGHHGGGGHGGGGGHHFHHHGGG@CGHHHGGGGGGGGGGGEGDGFFDHHGEHHBCFHGBGFAGGHFGGCEEF0BE///FDFACF?FHFGC//??1B0<FF>DFCFDFFFFFFFFF-BBAFFFFFFFFE@D9B;FBFFFFF -@M01368:8:000000000-A3GHV:1:1103:25465:20010/2 -AACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGTGCACCCTATGTC -+ -AAABBFFFFFDFGGGGGGGGGGHHHHHHGHHHGFHHHHHHHGGGGGHHHHHGHHHHHHHHEHHHHHGHGGHHGGGHHHHGGGGGHHHHHEHFGEHHHEE14BGHHHGHGHFCE/EFGGGGGGHGGGGGG?FFGGGG.ADGGGFFDA.FFEC--;9BBBD?FFFFF -@M01368:8:000000000-A3GHV:1:1103:25519:6301/2 -CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATGCTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGGGATGTCTTATTT -+ ->AABBFFFFFFFFFFGGGCAFGCGHGGGHGGGHHFHHHFHHGGGGGFFFHHHBHHHHG5BG5GFEEEE1FGFFGF1EGHGGG/BFHEF@EA1BE1@EED3?4DHGGGGEGHFFFDDG?DCDEG@<?@DGCAGC-;@DGFFHGCHEEFGGFGFB/;..BBB.CCE?A0FE0EBFF0FEF.;=DFFB//BFBF//;/:9BA.9FB9//;9//;9F;FF -@M01368:8:000000000-A3GHV:1:1104:13832:4398/2 -GACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTG -+ -BBCCCFFFFFFFGGGGGGGGGGGGGHGGGGGGGHHHGFGGHHFGGGHGHHGHGFGECGHHHHGGGGGGGHEGFGGGHHGHFHHHHFGHFHHHHGHHHHFGGFGG3FFGGHHHHHHH2?FFGHFHHHHHHHHHBGHDGC0 -@M01368:8:000000000-A3GHV:1:1104:23670:4161/2 -CGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTT -+ -A3AABBFFFFFFGGGGGGGGGGHHFHHHHHHGHGHHHHHGCHHHHGGGGHHHHHHHHHHHHHHHGHHGHGHGGHGGHGHGGHHGFGHHGHHHEHHGHHHHHGHEHEHHHGGFGGHHHFHHHGHHHHHHHGHHHHG4EGGGFCBBCA?DGGGGGGCCAFFFFFFFFFFFFFFFFFDFFF.9@--=AFFFF;FBFFBEFFF9FBFFB9/FFFFFFB/FFFFFFFBFFFF/BBFBB/FFFE?.;@FFB?FB -@M01368:8:000000000-A3GHV:1:1104:24927:22253/2 -GTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGGGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGGGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAA -+ -AABBBFFFFFFFGAFGG5GGGGHDGHHHHHHHHHHHHFHCFGC2F3BFGFGHBGHHFHEDHFGGGGGHHHG3F?GHHGGC/EFEHFGAEFFHGGAEHGGGG3EEGEEDHFHAAD@DGGGHG/AC1>GED-<<-.;C0:0C:C;GH:::::0B90;FFGEGFBFF0C.;9F0;0090;900;00FA9D.A...-9=BBFFFE/FE9;9E//BBBB.BBB///B/9/B -@M01368:8:000000000-A3GHV:1:1104:4705:7727/2 -TCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCG -+ -ABBBAFFFFFFFGGFGGFGGGGGHHHHFHHHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHEDEFGHFHHHHGHGHHHHHHHGHHHHHHHHFHGHHHHHGGGGGHHHGHHHHHHHHHHHGHHHHFHGHGHGGHGGGDCD.;FFGGGGGGGGFFFFFFFFFFFFF.>FFFFFF-BBEFFFFFBFFFFF-DDFFFFFFEFFFFFFFFFFFF?F///9FFFFF/;FFFFF?AADCFFEFAFFEFFFFFB/;B/A9 -@M01368:8:000000000-A3GHV:1:1104:7756:4776/2 -CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG -+ -BBBBBFFBFFFFGGGGGGGGGFFHHGHHGHHGGHGHHGFHHGGGGGHHHHHHHHGFF3GGGHGGGHFDHEGHHFHHHHGFHBCGHGEGFFFFFHHHHHGHHHHHGHHHGGGGGHHGHHGGHHHGGGGGGHHECCFDHHADGGHHHFGFDC////FHHHDGFCGCDEECF?EGGFFHHFEHFGGHHFFF/A900;C.9..C0990.;CFBFGE/9.9B99/;B.99/FB///9AEF/99/9/BBF/B/B//A -@M01368:8:000000000-A3GHV:1:1105:11310:21761/2 -ACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCTC -+ -BCCCCFCCCCCCGGGGGGGGGGHHHHHHHHGGGGGHHHHHHHHGHHHHHHHHHGHHHHHHHHGHHHHHHHHHHGGGGGHHHHHHHHHHGHHHHHHHHHHHGGHGGHHHHGHHHDGHHHHHHHHHHHHHHHHHGHHHHHGGGGGGHHHHHGHHHHHHHHFFHHHHHCCAHFHHGGFFFFFFFFFAA;BDDFFFF00.-=D.ABDA-.::AAF;F@.AAD./0000.9-;.9:00009:F090;FF000900 -@M01368:8:000000000-A3GHV:1:1105:11578:14676/2 -GCTCCGGGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTAT -+ -CCBCCCBBCCCCGGGGGGGGGGHGGGGGHHHHHHHGHHHHGHHHHHFFFGGHGHHHHHHHHHHHHHHHHFGHHHHHHHGHHGHFHFGHHHGGGGHHHHHHHHHHHHHHFHHHHHHHGGHGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHDGGCGHHHHHHHHHHHHHFF>ABFDCFFFFFDGFGFDGDEG1EGEFFBFHFGHA0E00A0CAAC0FFDFFAGHFEHHFDH/EAFCFFHACGHHAFFEF2DFFG/FGHHHHGHGH0C@/E@GGC0FG10>GGFGHHGHFFBGFGB2>F@;-A@FF<-;---@-;-----;-BFF/;---9//BBFFFF//B;FF///9BF///;/;B/BBFBFFBB/;-9--///;9/:-;BFFBBB9F -@M01368:8:000000000-A3GHV:1:1105:15502:11453/2 -GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGAC -+ -CCCCCFFFCFFFGGGGGGGGGGHHHHHHGHHHHHGGGGGFHFHHHHHHGHHHHHGHHHHHHGHFHGHGGGGGGGGHHHGGGGGGGGHHHGHGGGGGGGGGGGHHGGGFGGGFEGGGGFFFGGFGDGGGBFGFFFFFFFFFFFFFE0BFFFFFFFFFFFFFFFFFFF;BFFDFFFFBF0BFFBFFEEBFFAA009FFFFFBFFEBB0;.BFFBFFFFFFFFFFFF0;BFFFF -@M01368:8:000000000-A3GHV:1:1105:16787:9771/2 -TCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAG -+ -CCCDCFFFFFFFGGGGGGGGGGGHHHHHHHHHHHGGGGHHHHHHHHHHHHHHFHHHHHHHGGFGGHHEHHHHHAGHHGHBHHHGHHHHHHGHGHGHHHGGGGGHHHHHHHHHHHHHHHFFHHHHHGHGHGHHGGGACAFGGGGGG-AFGGFFFFFFFFFFFFFFFFFFFFF-9@AFEDFFFFFB.9-AABBBFFFEFFFFFFFFBFFFFFFEBB0BBBFFBFFBFBEDEAFFEB.ABFBFBFFFF0:: -@M01368:8:000000000-A3GHV:1:1105:17564:15723/2 -ATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTT -+ -CCCCCCCCCFFFGGGGGGGGGGHHHHGHHGHHHHHHGHHHHHHFHHHHGGGGHHGHFHHHHHHHFHHHHHHHGHGGHGGHGHHHHFHFHHGHHHHHHHHHHHHHEHGHHHHHGGGGGHHHHGEHHGHHHHHHGHFGHFHGGAHGGHGGGCCBDFGFFAFDBDDFFFFFBDA;AAD-ADC?AFEFB-@@AAEFEFFFFF..-:.B0:FFFFFFEF0BFF09FF.0;9BFFFFF0BFFFFFF?.::-909;.9 -@M01368:8:000000000-A3GHV:1:1105:18108:12091/2 -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTA -+ -CBBCCFCCCCCFGGGGGGGGGGHHGHHHHFHHHHHHFHGHHHHFHFHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHGHHHHGHFHGHHGHHHHHHGHHHHHHGHHHHHGGGGGHHHHHHHHGHHHHHGFGHHHHHGHDHGHHGCA;?AAFGFEFDFFF.FFFFEBDFFFFFFFFBFFFFFFFFFEFEFFFEFFFFFFF.:BFFF0BFFFFFFFFFFFF;0FF0;BFFFFF -@M01368:8:000000000-A3GHV:1:1105:18331:16683/2 -TTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGGGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATAT -+ -CCCCCFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHGHHHHHFHGHHGHHHHHGGFGHHIIHHIHHHHHHHFHCFGHEEHFFHGHHFGHGHGHFHHGGGGGGEEGHHEHFHHGGHFHHFGGGGDFGBCGG?DGHGGFCGGHHECEF?CHGGDGFFGGHHC?EEGGHHHH.>AEGGGHEE-AEG0C0GCCEFFFFGEFG/;CEGGGE.:CAE.;@.@EFBFFFF/..:BFF0:BB00FBFFFFBDAFFF0; -@M01368:8:000000000-A3GHV:1:1105:20577:18421/2 -ATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGCCGAAAATACCAAATGCATGGAGAGCTCCCGTGAATGGGTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATT -+ -?ABBBFFFFFFF4CFFGGGFGGHHHHHHHCEGHHCHFGHCAFEFHHHGGAEGGHHDHHHBGHBGHHH?5BGE1AEEFHHHHHGHFHBGHGEGGGGHBFHHFGHBGCFFG4EGHGG?E/FG3F3F/FGCDECEA/FGG/CEDBB//?BHEHHF0ADC@D>D9@DDCFFFFFFCFDFE.AA;/BB/.;-.;BFBBBFFBFFFFFFF/9FF?/;/BBFFFFF/;FFB -@M01368:8:000000000-A3GHV:1:1106:17389:18671/2 -GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTCTTTAGGGTTTATGACC -+ -BBCCCFFFCBCCGGGGGGGGGGHGHGHHGFGFGFHGGHHGHHHHHHHHFHHGGGGAGFBGHHHFBBGH5GDHH5EEEGEHEGFBF1DGFDGFGGGBBCEEGEHFEFDDHFBFHFD/@BGDGDHHGDCCFCD/C<->.ADDGE1GA;9.FFFFFFD-99ADFFD-.9FADF--9..;AAFEFFEAA-;.///BB:BBF/BBB//B//99/;BBB/9////F -@M01368:8:000000000-A3GHV:1:1107:18714:24788/2 -GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACC -+ -BCCCCCFFFFFFGGGGGGGEGGHHHHGGFFHHHHHHHHHHGGGGGHHHHGHHHHHHHHHHHHHFHHHHHHHHGHHHHHHGHHHHGGCGHHHHHHHHHHHHHHHGHHHHHGDGHGGHHHHHHHHGHHHHHHHHHHHGHHHHHHEHHHGHFGGGGHHHHEHFHHGHFHHF.>GGHHHGHGHGGHCFFCDFDFFFFFFAAF.FFFFFEFAFCFFA..@.AFFFC9--9;. -@M01368:8:000000000-A3GHV:1:1107:21355:8333/2 -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCGGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTA -+ -AABCCFCCCCCFGGGGGGGGGGHHHGHHHFHHHHHHGHGHHHHHHGHHHHGGGGHHHHHHHHHFFHHFHGHHHHHHGGHGGHHHGHHHHGGHFHHHHHHHFHHGHHGEFFHFHHGGGGGGHHHFHHHHGHHHHE?FGHHHHGFGHGHHGGGDGGGGGFFFCFFFFFFFFFFFFA;DBBDFFFAFFFF;DBAFEDFFFFFEA?-9...9;9;BFFFF//BFBFFFFBFBFFFFFB/BFFF9B/EDEF-BF -@M01368:8:000000000-A3GHV:1:1107:22864:15712/2 -GTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATT -+ -AABCCFFFFFFFGGGGGGGGGGGGGHHHHHHHHHHGHHHGGGGFHGHHHHGHFGFFFFGGHGGGGGCHFHFAFEDHHGGFGGGHHEGEGGHHEGGCHGHHHHGGGGGGHGF0EECGGGGFECCFFGFBGHHHHHHGGHHGHFCFEHFBEFGGADDDC=CC0CCFHG:CEHHHGHBGH:GFFGFF0CAAA/BF09BBBBB -@M01368:8:000000000-A3GHV:1:1107:24817:14233/2 -CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAAAGTGTGGGCTATTTAGGTTT -+ -CCCCBFFFFFFFGGGGGGGGGGFHHHHHHHHHHHHHHGHHHGEHGGHHGHHHHHGHHEHHGGGGEHGHHHGEHHGHHDGHHHHGGGGGFGEFGGEGHHGCEEEGHHEFGGDHGGGGAGFHHHHGGF/?//GHFFDGGGGGHAD??DC1DHHBGHHHHGHF<FGGGGGGGGGGGFHHEFGHHHGGGGGGHHHCGGEFHFGGGFHHHHHGGGEGGHHFHEEGEG/EEEE/?/EHFHH3GGH4EGHHCB01CGHGHGD//<@CGGBFFDGHHHH0FEHHGEGFHCD11>0=GGHGCCFGHGBGGD0GGHC0C.@?ACEEC/.EDEB?BFFCFB9CFF090BBCBFBF0CCB0B00A.9E.B/9BA=-..B9FBF//;BBBBFF -@M01368:8:000000000-A3GHV:1:1108:11059:23181/2 -AACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCAC -+ -CCCCCFFFFFCCGGGGGGGGGGHHHHHHHHHHHGHHHHHHHHGHHHHHHHHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHHHHHHHFHHHHHHHHHHHHHHHHGHGHEHHGFCGGGHHHHHHHHHHHHHHHFGHHHHHGHGHGHHGGGBFGFFFFEAFFFA.BFFFEFDDFFFFFFFFFFEEFF;D;EFFFFFF0BFFFADDFFFFFBFBFFFFFBB0FFFF0::0BF000BFFFBBF0EDFF -@M01368:8:000000000-A3GHV:1:1108:12233:13498/2 -GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTA -+ -ABBBCFFFFFFFGGGGGGGGGGHHHGHHHHHHBGHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHGHHHHHGGHHGHHHHHHGHHHHHHHGGHHHHGGGGGHHHHHHHHHHHHHGHGHHHHHHGHGHGGHGGGGAGGFGGGGGGGGFFFFFFFFFFDFFFFDFFFFFFFFF>.AEDEFFFFF?E-DAB9FFFFFFFEF:FBFB009:0FFFF9BFFBFFFFBB0.9A=-/0 -@M01368:8:000000000-A3GHV:1:1108:14328:14751/2 -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTAT -+ -ABBBBFBBBBBFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHDHHHHGGGGHHHHHHHHHHHHGHHHHHHHHHGGHGGHGGHHHHHGHHHHHHHHHHHHHHHHFHGHHHHHGGGGGHHHHHGHGHHHHHHG?FHHHFHGHGHGHHGGGGGGGGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFAFFE.ADEFFFFFFFADFFFFF///BBFF/FFFFFBFAFFFFBB///;9: -@M01368:8:000000000-A3GHV:1:1108:16350:22621/2 -CATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATAT -+ -CCCCCFFFFFFFGGGGGCGGGGHHHHHHHHGHHHHGHGHHHHGGGGHHHHHHHHHHHIHHHGHHHHHHGGHGGHHHHGHGHGHHHHHHHHHHHGHHHHHHGGHHHHGGGGGHHHHHHHHHHHHHGHGHHHGHHGGGGGHHGGGDADDGFFGGGGFFFFFFFFFDFFFDFFFFFFFFFFFAFAEFEDFFFFFFEE-AAFFFFFBFFFFF0FFFBFF;AFFFFFFFFFFFFFFFFFFFFFAB9BFFFFFBFFF -@M01368:8:000000000-A3GHV:1:1108:21791:23272/2 -CATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTAT -+ -BBBBBFFFFFFFGGGGGGGGGGHHHHGGHHHHHHHGGGEGGHHFGDHGHHHHHHGGHHH2GGHHHGHHGGGGFGFBFHHHGGGGHGHGHHHHHHHHHHGHGHHGHHGGHGGHHHHGGFHGGGHFHGHHHHHGGHHHGHGFCGFFDGGG/FCFHBGHHHHHHHGHCHHHHGGGHGHGHGCFF;>@DFFBFFFFFFDFFFFFFFF;;.DDAA;FFF;BB;C@..9::BBFBBA;-:.:: -@M01368:8:000000000-A3GHV:1:1108:23314:26089/2 -CATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATT -+ -BBAAAFFFFFFFGGGGGGGGGGHHGDHHHHHFHHHHGGGGGFHFFGFFHEGHHHHHHHHFGHFHHHHDEHHHHHHGHFHHGGGGHHGFHHHHHHHHHHHHHHHHHHF?EEFCHFGFHHEFFFHGFHHGHFHFHHHFFHAG??GHACGGDGHGGBFHHHHHHHGHFGHGHGEGHGCFFF@CD-BDDFFFFFFFFDDFFBBFFD-B-A.DFFA.AFEFF-;-..:..BFFFFFA-../9FFF//B/BBBBF -@M01368:8:000000000-A3GHV:1:1108:25084:18127/2 -CCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAG -+ -CCCCCFFFFFFFGGGGGGGGGGGGHHHHHHHHFHHHHHHHHHHHHHHGGHHGHHHHHGGGGFHHHHHFHHHHHHHHHGGHHHHHCGGHGHHGGGGGGGHHGGGGGGGGHHHGHHGGGGGGGGGGGGGGGG@CDE?EFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFFFE;DFBFFFFFFFFFFFFFFFFFFFAFFFFFFFBFFE0BBBFFFFFFFFFFBFFFF9BB0:0:B; -@M01368:8:000000000-A3GHV:1:1108:7626:10048/2 -AACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGAT -+ -BCCDDFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHHHHHHHGGGGGHHHHHHHHFGHHHHHHHHHHHHGHHHHGGFHHHFGGGGHHHGHHHHHHHHHHHFGHHHHHGHGHGHHGCGCGGFHHHGGGGGGGGGGGGGGGGGGGFFFAFFFFFFAA-AFEFFFFFFFFFFFFFFFFFFFFFFFFF -@M01368:8:000000000-A3GHV:1:1109:11270:12255/2 -CTACCTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATT -+ -BCCCCFFFFFFFGGGGGGGGGGHHHHHFHHHHGGGGHHHHHHHHHHHHHHHHHHHHGHGGHGGHHGHHHHHGHHHHHHHHHFHHHHHGHHGHHHHHGGGGGHHHHHHHHGHHHHHH?FHHHHHGHGHGHHGGGGGGGGGGGGGGGGGGGGFFFFFFFFFFFFFFFFECADFFFF.DEBFFFFFF;D.B;.ED.FFFFFFF-.:00 -@M01368:8:000000000-A3GHV:1:1109:24217:19077/2 -TAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGAACCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTT -+ -BBBBBFFFFFFFGFEGGCFGFGHHHHFHHFHHHHHHGGEEEFGHGFFAGFFEEAEGGHHBEEFAFGAEF1G3GFGGEEF@EAGGHGFF?ECGGHEEEFGGHHHHFHHFHHGFHHHC2DAGHGG00-BFFA9FFFFFFFFFFFBF -@M01368:8:000000000-A3GHV:1:1109:27103:7153/2 -NTACTTCAGGGTCNTAAAACCTNAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCANGGNAGCTCTCCNNGNNNNTGGTATTNTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTAC -+ -#>>AAAFFFFFFG#AAFFGGGG#BAFFAGHHHGGGGHGHHHHFHHHHHHHGHHHHHHHEGFGGHHBGHGHHBGFFEG2GHHFHFHHHGHHGHFH#/B#??FFGHHH##?####????FHG#??CFGGGEF>CGAGAGGGFGAA@@FFFFFFFFB=@@FF?FF;FFFF@@-@-AFEF?BB/BF?E-BBFFF;:BB/BFFFB/BFFFBFBFBBFFFFFB/FFFBB/;9A@@BFFBFFFF/BBFFFF -@M01368:8:000000000-A3GHV:1:1109:8763:7859/2 -GGTTCCTACTTCAGGGTCATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAG -+ ->AAABFFFFFFFGGGGGGGGGGHHHHHHHCHFAFHHDGEGFHHHHHHHHFHHHHFHHHHHHGHGGHGGHHHFHGHHHHHFFHHHHHHBGHFFHGHGHHHHHGGEGGHHHHHGHHHHHHHHHGFGHHHHGHGHGHHCCGGGGGGGGGGAAGF?FFFFBFA@=BDDFF?-;FFF;@CFFEFEFFFFFFFFFBFDFFFFFFFFFFFFFFFFFFFFFF:FFFF/BF/FFFEFF?DECAFEFFFFFE:FFFFFFFB -@M01368:8:000000000-A3GHV:1:1110:12808:18367/2 -GCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACAGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATT -+ -ABCCCCDDDFFFGGGGGGGGGGHHHHHHHHHGGGGGGHHHHFHHGHHHHHHHHHHHHHGHHHGGHHHH3GFHEFHHHHHGHHGFHHHHHFGHHHHHGHGHGGHGGGGGGGHHHGGCGGGGHHGGGGGGGGGGGGGGGGGGGF-BAEFEBEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFFAFEFDFFFFFFFFFF0BFBED=@DFFFFFFFFFEFFFFFBFAFB;0: -@M01368:8:000000000-A3GHV:1:1110:14751:26540/2 -ATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGGTTGGGCTATTTGGGTTTTTTGACC -+ -BBBBBFBBBBBFGGGGGGGGGGHHHHGGFHGGHGGCHHHHHHGHHHGHHGEGGGHHHHHHHHHHEFHHHHHHGEE?FGHGEHGGHHHGGGGGGEHHGGGGHHGGGEGHHHGHGGGDFGHHGHGGGGGCGAFFCCDFHHHH1CGGHBHFHFCGFFCDFHGCCE.CC:EGFHHCGHHGCF?FGGGFGGG;B9;FFFFFFEGEBF0;;;0;CFBFFF?D.DE.:..99-...;;//9A.;DE.9-./: -@M01368:8:000000000-A3GHV:1:1110:15519:10008/2 -GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACA -+ -BBCBCFFFFFFFGGGGGGGGGGHHHGHHHFHHBGHHHGGGGHHHHHHGHHHHHHHHHHHHHHHGGHGGHHGHHHGGBGHHHHHHHHHHHHHHHHHGGFHHHGGGEGHHHHHHHHHHHHHHHGHHHHHHGHGHGHHGGGGGGDGGGGGGGGGBFFFFFFFFFFFFFFFFFFFECB-=BFFFFFFFFBB?E-A::FFBFFFFFBFFFFFFFFFFFFBFFEFFF;0BFF0:FE?FFFFBFFFFFFFFFFFFFF -@M01368:8:000000000-A3GHV:1:1110:16559:7507/2 -GCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAAT -+ ->AABBCCCCFFFGGGGGGGGGGHHHHHHHHHGGHGGHGHFHHHHFHHHHHHHHHHFHHGHHHHGHHHHHGGEEGHHHHHHHHHHHHHHHHHHHHHHGHGHGHHGGGGGGGHHGGGFGAGFGGGGGGGGGGGDDDFAGGFFFFCFAFEEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFFFFFFFFFFFFFFE..@BFFBD./;:0FFFFF09B/AFCA=-99BFFBFFFFFFFB:BFBFFBFB9: -@M01368:8:000000000-A3GHV:1:1110:16700:6843/2 -GTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTT -+ -CBCCDFFFFFFFGGGGGGGGGGHHGHHHGGHFHHHGGGGGHHHHHHHHHHGHHHHHHHHHHHGGHGGHHHHHHHGBGFFHHHHHHGHHHHHHHHGHHHHHGGGGGHHHHHHHHHHHHHHHFHHHHGHCG/GGHHGGGGGGAFGEEADAGGDFFFF00D>CFDDFFFB?AF?B;-@>.FEDFFFFF0BF-BDFFFFE00B0BF0FFFF:BFA0B0:BBFBBFFFFF -@M01368:8:000000000-A3GHV:1:1110:18014:7856/2 -CATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTAT -+ -CDDDBFCDDDDFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHGGGGGHHHHHHHHHGHHHHHEGHHHHHEHGHGHHGGGCCGGHGHGCGGGGDHHHHFHGGGGGGGGGGGFGGFGAC-EFEGGGFFFGFF;DFFFBFFFFFFFFFFFFFFFFFFFEFFFBFFFFFFFFF -@M01368:8:000000000-A3GHV:1:1110:23303:11846/2 -GTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTAAGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTA -+ -BBCBBFFFFFCFGGGGGGGGGGHHHHHHGGHHHHHHHHGH5GHHHGGGGHHHHHFHHHHHHHEHHGFBGEEFE1FG5GFHHHHHFHHHGGGGGHHHHHHHHHHHGGHHHHHGGGGFHHFHH/EHHHGG?@EFHHHGGGGHFGGGGHHFHGGDGGGGFFFHGFDGGGCGACC;DFFB0CH0CCCFGBFBFB/:A?FFFE?AA.CFBECE0BBBF0F/A./9FFFFF./;/FFFFFFA.:////9BB/B/BB -@M01368:8:000000000-A3GHV:1:1111:13195:14562/2 -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGAT -+ -ABBBBFBBBBBFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHDGHHHGGGGHHHHHHHHHHHHGHHHHHHHHHGGGGGHHGHHHHGFGFHHHHHHHHGHHHHHHHGHHHHHGGGGGHHHHGHHHHHHHHHHGFHHHHHGFGHGHHGGGGFCGFGFFFFAFFFFFFFFFFA;BADFFFAFFFFFFFFFEFFFEFFFFFFF;FDBFFFFFFFFFFFF -@M01368:8:000000000-A3GHV:1:1111:14287:20817/2 -ATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATGAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGTTAGACCTGTGATC -+ -CCCCBFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHHHHHHHHHGHHHGHHHHGHHHGHGHFHHHGGGGHHHHHHHHBGGGFHHFGGHHGGHGHHHHHHHHEGFFHHFGGGGGHHEHHHBHHHHHFFHHGHGGGGGHFGF3FDGHHGCGGFGHHGGGG/2GG@E?GHHHGFEDGGCFDFFFFFFFFFFFBFFFFFFFFFFF/FFF/BFFFFFFFBA.?@;FFBDFFFF -@M01368:8:000000000-A3GHV:1:1111:7437:24508/2 -ACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGT -+ -BBBBBFFBABBBGGGGGGGGFGHHHHHHHHHHHHHGHHHGFHHHHGGGGDHHHHHHHHGFHHHHHGHHHHHHGHFHGHHGGGDFGDFFGGGFFGFFGHGHHHGGC?AD-:A-AFFGGG-?9EFEFGGFFBFEFCFFFFFFFBBBFFFFFFFFFFFFFFFBBFFFFFFFFFFFFFFFFFFEFFFFFFBFFFFEFEFFFF;CF-FFFFFFFF/BFFFFFFF -@M01368:8:000000000-A3GHV:1:1111:8665:25542/2 -CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGACAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTT -+ -CCCCCFFFFFFFGGGGGGGGGGHHHHHHHFHHHHCHHGDGGGGHGGHHHHHHHHGHGHHHGGGGGHFHHHHFHHHFFHHHHHFGCGGEHFHHFGGHFHGGGGGGHHFEEEAFEFFDFHGHHHHGGGGGGGHH0DGGGGCGHCC-FFFFFFFGGGGGGGGGGHHHHHGHHHHGHHGGHHGGHGGHHHGHHHHFHHGHHAFGGGHHHHHGHHHHFHFHHHHHECGGGHGHHHEFHFHGGGGGGGGFGFFGFFEFGEGHHHGHHGGDGGGHGFGGGGGGGGE@GCGGHHHGHHHHHGHHHHFHHGEGFFEGCE.ECCECEHCGGFFE/AABFFG0CFE0BFFF9CFGG.F/BBBFFGFGGGFFFFFFBFEDAEFFFFFBFFFFFFF0;FEBF: -@M01368:8:000000000-A3GHV:1:1112:10272:7828/2 -GTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATAC -+ -BBCCBFFCCCCCGGGGGGGGGGGGGGHHHHHHGGGGGGHHFHGGGGGFGHGGGGGGHHHHHHHHHHFHHHHFHGFFHHGF?ECEFFFFFFHHHHHH?FHHHHHHHHHGFHHHHHHGGGFGHHHHFHHHHHHHHHHGGGFGFGCGGGGHHHHHHHHHHHGGFFHGHHHHHFFHFCGFHCCGHGHCGGHG?DGG -@M01368:8:000000000-A3GHV:1:1112:9832:16531/2 -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTA -+ -BBBBBFBBBBBFGGGGGGGGGGHFHHHHHHHHHHHHHHGHHHHGHFHHHGEEGGHHHGHHHHHHHHHHHHEHGHHGGGHGGHGHHHHHGHHHHGHHHHHHHHHHHHHHGHHHHHGEGCGHHHHHGHHHHHHHHHHHHHHGHFFGHGHHGGGDACDFFFFABFFFFFFFFFFD-BC?DA9.DFFBEA==B=?F.;ABBFBFEF-DAF/BFF9/FFFBB/9BFFFFFFF/BFBBFFFFFFF/9FEA?--B/ -@M01368:8:000000000-A3GHV:1:1112:9832:6701/2 -GCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATT -+ -BBBBAFFFFFFFGGGGGGGGGGFGHHHHHGHHHHHHHHHHHHCGHHHHHHHGHHHHHHDGHHGGAECHHGHHHHHHHGHHHGHGFHHHHGGHGGHHFHHHHHHFHHHHHHHHH2GHHHHGHGHHFHHGGFGGGHHGHHHHHFHHHHEFHHHHEFGFFHGEHCFGDFDFFFFFCFFFFFFFFFFFFFAFBFFDFFFFFFFFADCF?9?EFBFB9EF-A.;BFFFFFFFBFFFFFFFFFFFFBFFFFFFFFFF -@M01368:8:000000000-A3GHV:1:1113:13532:8618/2 -GCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTGGAT -+ -CDDDBFFFFFFFGGGGGGGGGGHHHHHGGHHHGHHHGHHGGGGEHHGGHHHHHHHHHHHHHHGHHGHHHHHGGHHHHHFHHHHGCDCGHHHHHHHHEHHHGHGHHHGFHGGHGGHHHHHHHHFHHHFHHHGHHHHHHHHHFGGHHHHGGGEGAFHHGHHGGHHHHGHFBGFHGHGHGHGGHGGF@DAFEFBECAB=A.0BFFFEDF=A9.@D.-AAFFFA-:-..:.9/0:;0..-..0000::BFB09:: -@M01368:8:000000000-A3GHV:1:1113:21679:18011/2 -TTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATC -+ -BCCCBFFFFFFFGGGGGGGGGGHHHHHFHHHGHHHGFGHGGHGGHHHHHHGHHHHHHHGGGGFHHHHHHEGHHHHHHHHFGGFCGEHFHFGEGHHHFGGGGGHHHGGGEHGGGGGHHHHHHGDCCCGHHHHDGGGGCD/FCC->DFF -@M01368:8:000000000-A3GHV:1:1113:5741:16959/2 -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTA -+ -BBBBCFCCCCCFGGGGGGGGGGHHHHHHHGHHHHHHHHGHHHHGHDGHHHGGGGHHHHHHHHHHHHHHHGHHHHHGGGEGGHHHHFHHHHHHHHHHHHHHHHHHHHHHGHHGHHGGGGGHHHHHHHHHHHGHHHFHHHHHHGHGHGHGGGGCGGFGGFFFFFFFFFFFFFBFFFFFFFFFFFFFFAFFFFEAEFFFFFFFFFFF9BFFBFFFFFFFFFFFFFFBFFFFFFFFFFFFFFFFFFADAB-/B -@M01368:8:000000000-A3GHV:1:1114:10130:11959/2 -ATCAGAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACG -+ -BCCDDFFFFFFFGGGGGGGGGGGHHHHHGGHHHGGGCGGHGHGGGGHHGGGGHHHHHHGGGEGGHHHFGGGGG?E1FE?/EEHHHHHGHHGHHHHGHFHGHGHHGDGGFG2FF2?GHHHHHGCCCFHGHGHHHHGHHFEHHFGHHGHH<1=DGHHHGHHGHGAGAE -@M01368:8:000000000-A3GHV:1:1114:14540:5315/2 -CACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGC -+ -CCCCCFFCCDCCGGGGGGGGGGHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHHGHHHHHHHGHHHHFHFHHHGGGGGHHHHHHHHHHHHHGHHHHHHHHGGGGGGHHHFHHHGHHHHHHHHHHHHHHHHHHGHGHHHGGGFGHHHHHHHHFHHHHHF?1FHHGHGHGHGHHGGFFFFDBFBE;BCC.:BFFFFFFFFFFFFFF;AFFFFF-=-.AEDEFFFFF..9A;9FFFF0FFFFE00FFF0:B -@M01368:8:000000000-A3GHV:1:1114:15066:16302/2 -TTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAG -+ -CCCCCFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHHHDGHHHHFHHGHHGHHHHHGGGGEHHHHFHHFF5FHHFEGHHHGDHGGHGHGFGGGEHFGHHGGGGGHGHHHGHHFHHB3FGHHFGGGG?GFFHCCEBGFFECCDFEGFCFGCHHGFDDHHHGHHCFGGGGGFBFDGFG?-:..AFG.-C0C009;:00;00:9/:CEFFF?AE::9;9?0:FEF0; -@M01368:8:000000000-A3GHV:1:1114:16639:15258/2 -TTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCGCACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGAT -+ -CDCCCFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHGHHHHHHGGGGGHGIHHHIH5DEGHHHF?FGHGGHGGHEGGHFHHGHGEHHGGGGGFFFGHFBG2GHEBGHHGHGGEG/GFGABEDFGHEED?GGHHFFGGGCFEGD/GFHFFGEFGCGG?CC??D-EF@EEEFGCDDBBFGGGEBBFFF09090A.BFGA.9CCA0;EBAB00BBFF.@-./;BB;BFFF0:00099AAFFF -@M01368:8:000000000-A3GHV:1:1114:2404:13066/2 -ATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGACCATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGA -+ -CCCCCFFFFFFCGGGGGGGGGGHHHHHHHHHFFHHHHGGGGGHFFFHHFHHHHHHHHHHHHHHHGFEGGGHGEDFCDFHGHFG@@DGGHHHHHHGGGGCGGGGGEHGGCGBB?CF99EGFGGFGG?D9CFFFF/BBFFFFFEF9BFFAFFFFEFFFFFFFFFFFFFFFFFFFFF.FFBBFFFFFFFFFFFF-9;;;BFFFFFB9BFBFBFABFFEFFFFFFFFFF::BFFBFF diff --git a/tools/trim_galore/test-data/paired_example_results2.txt b/tools/trim_galore/test-data/paired_example_results2.txt index b088f08509..96f1f64acc 100644 --- a/tools/trim_galore/test-data/paired_example_results2.txt +++ b/tools/trim_galore/test-data/paired_example_results2.txt @@ -1,50 +1,38 @@ - SUMMARISING RUN PARAMETERS -========================== +========================= Input filename: input_1.fastq Trimming mode: paired-end -Trim Galore version: 0.6.10 -Cutadapt version: 5.0 -Python version: 3.12.10 -Number of cores used for trimming: 4 +Trim Galore version: 2.2.0 (Oxidized Edition) Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 -Using Nextera adapter for trimming (count: 29). Second best hit was smallRNA (count: 0) -Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) -Maximum trimming error rate: 0.1 (default) +Adapter sequence: 'CTGTCTCTTATA' (user-specified or auto-detected) +Maximum trimming error rate: 0.1 Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp -This is cutadapt 5.0 with Python 3.12.10 -Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq -Processing single-end reads on 4 cores ... +Trim Galore 2.2.0 (Oxidized Edition) — adapter trimming built in +This is cutadapt 4.0 (compatible; for MultiQC backwards compatibility) +Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq +Processing reads on 1 core in single-end mode ... === Summary === -Total reads processed: 99 -Reads with adapters: 52 (52.5%) -Reads written (passing filters): 99 (100.0%) +Total reads processed: 99 +Reads with adapters: 52 (52.5%) +Reads written (passing filters): 99 (100.0%) -Total basepairs processed: 24,849 bp -Quality-trimmed: 205 bp (0.8%) -Total written (filtered): 23,339 bp (93.9%) +Total basepairs processed: 24,849 bp +Quality-trimmed: 205 bp (0.8%) +Total written (filtered): 23,339 bp (93.9%) === Adapter 1 === -Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times +Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times. -Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-12 bp: 1 -Bases preceding removed adapters: - A: 9.6% - C: 38.5% - G: 23.1% - T: 28.8% - none/other: 0.0% - Overview of removed sequences length count expect max.err error counts 1 11 24.8 0 11 @@ -55,78 +43,70 @@ length count expect max.err error counts 13 2 0.0 1 2 14 1 0.0 1 1 16 1 0.0 1 1 -17 1 0.0 1 0 1 -20 2 0.0 1 2 -21 1 0.0 1 1 -24 1 0.0 1 1 -26 2 0.0 1 2 -31 1 0.0 1 1 -33 1 0.0 1 1 -41 2 0.0 1 2 -49 1 0.0 1 1 -50 1 0.0 1 1 -54 1 0.0 1 1 -56 1 0.0 1 1 -58 2 0.0 1 2 -60 1 0.0 1 1 -67 2 0.0 1 2 -68 1 0.0 1 1 -69 1 0.0 1 1 -73 1 0.0 1 1 -80 1 0.0 1 1 -86 1 0.0 1 1 +17 1 0.0 1 1 +20 2 0.0 2 2 +21 1 0.0 2 1 +24 1 0.0 2 1 +26 2 0.0 2 2 +31 1 0.0 3 1 +33 1 0.0 3 1 +41 2 0.0 4 2 +49 1 0.0 4 1 +50 1 0.0 5 1 +54 1 0.0 5 1 +56 1 0.0 5 1 +58 2 0.0 5 2 +60 1 0.0 6 1 +67 2 0.0 6 2 +68 1 0.0 6 1 +69 1 0.0 6 1 +73 1 0.0 7 1 +80 1 0.0 8 1 +86 1 0.0 8 1 + RUN STATISTICS FOR INPUT FILE: input_1.fastq ============================================= 99 sequences processed in total - +Sequences removed because they became shorter than the length cutoff of 20 bp: 1 (1.0%) +Sequences removed because they were longer than the upper length cutoff: 0 (0.0%) +Sequences removed because of too many N bases: 0 (0.0%) SUMMARISING RUN PARAMETERS -========================== +========================= Input filename: input_2.fastq Trimming mode: paired-end -Trim Galore version: 0.6.10 -Cutadapt version: 5.0 -Python version: 3.12.10 -Number of cores used for trimming: 4 +Trim Galore version: 2.2.0 (Oxidized Edition) Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 -Using Nextera adapter for trimming (count: 29). Second best hit was smallRNA (count: 0) -Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) -Maximum trimming error rate: 0.1 (default) +Adapter sequence: 'CTGTCTCTTATA' (user-specified or auto-detected) +Maximum trimming error rate: 0.1 Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp -This is cutadapt 5.0 with Python 3.12.10 -Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq -Processing single-end reads on 4 cores ... +Trim Galore 2.2.0 (Oxidized Edition) — adapter trimming built in +This is cutadapt 4.0 (compatible; for MultiQC backwards compatibility) +Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq +Processing reads on 1 core in single-end mode ... === Summary === -Total reads processed: 99 -Reads with adapters: 58 (58.6%) -Reads written (passing filters): 99 (100.0%) +Total reads processed: 99 +Reads with adapters: 58 (58.6%) +Reads written (passing filters): 99 (100.0%) -Total basepairs processed: 24,849 bp -Quality-trimmed: 745 bp (3.0%) -Total written (filtered): 23,035 bp (92.7%) +Total basepairs processed: 24,849 bp +Quality-trimmed: 745 bp (3.0%) +Total written (filtered): 23,035 bp (92.7%) === Adapter 1 === -Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times +Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times. -Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-12 bp: 1 -Bases preceding removed adapters: - A: 12.1% - C: 37.9% - G: 8.6% - T: 41.4% - none/other: 0.0% - Overview of removed sequences length count expect max.err error counts 1 16 24.8 0 16 @@ -142,29 +122,39 @@ length count expect max.err error counts 16 1 0.0 1 1 17 1 0.0 1 1 19 2 0.0 1 2 -21 1 0.0 1 1 -25 1 0.0 1 1 -30 1 0.0 1 1 -32 2 0.0 1 2 -34 1 0.0 1 1 -36 2 0.0 1 2 -38 1 0.0 1 1 -40 1 0.0 1 1 -41 1 0.0 1 1 -42 1 0.0 1 1 -43 1 0.0 1 1 -49 1 0.0 1 1 -51 1 0.0 1 1 -56 1 0.0 1 1 -57 1 0.0 1 1 -60 1 0.0 1 1 -67 1 0.0 1 1 -80 1 0.0 1 1 +21 1 0.0 2 1 +25 1 0.0 2 1 +30 1 0.0 3 1 +32 2 0.0 3 2 +34 1 0.0 3 1 +36 2 0.0 3 2 +38 1 0.0 3 1 +40 1 0.0 4 1 +41 1 0.0 4 1 +42 1 0.0 4 1 +43 1 0.0 4 1 +49 1 0.0 4 1 +51 1 0.0 5 1 +56 1 0.0 5 1 +57 1 0.0 5 1 +60 1 0.0 6 1 +67 1 0.0 6 1 +80 1 0.0 8 1 + RUN STATISTICS FOR INPUT FILE: input_2.fastq ============================================= 99 sequences processed in total +Sequences removed because they became shorter than the length cutoff of 20 bp: 1 (1.0%) +Sequences removed because they were longer than the upper length cutoff: 0 (0.0%) +Sequences removed because of too many N bases: 0 (0.0%) + +=== Paired-end validation === + +Number of sequence pairs analysed: 99 -Total number of sequences analysed for the sequence pair length validation: 99 +Number of sequence pairs removed because at least one read was shorter than the length cutoff (1.01%): + 1 +Number of pairs removed for N content (0.00%): + 0 -Number of sequence pairs removed because at least one read was shorter than the length cutoff (20 bp): 1 (1.01%) diff --git a/tools/trim_galore/test-data/paired_example_results2gz.txt b/tools/trim_galore/test-data/paired_example_results2gz.txt index 29e14a56c6..ba07518a69 100644 --- a/tools/trim_galore/test-data/paired_example_results2gz.txt +++ b/tools/trim_galore/test-data/paired_example_results2gz.txt @@ -1,51 +1,39 @@ - SUMMARISING RUN PARAMETERS -========================== +========================= Input filename: input_1.fastq.gz Trimming mode: paired-end -Trim Galore version: 0.6.10 -Cutadapt version: 5.0 -Python version: 3.12.10 -Number of cores used for trimming: 4 +Trim Galore version: 2.2.0 (Oxidized Edition) Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 -Using Nextera adapter for trimming (count: 29). Second best hit was smallRNA (count: 0) -Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) -Maximum trimming error rate: 0.1 (default) +Adapter sequence: 'CTGTCTCTTATA' (user-specified or auto-detected) +Maximum trimming error rate: 0.1 Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed -This is cutadapt 5.0 with Python 3.12.10 -Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq.gz -Processing single-end reads on 4 cores ... +Trim Galore 2.2.0 (Oxidized Edition) — adapter trimming built in +This is cutadapt 4.0 (compatible; for MultiQC backwards compatibility) +Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq.gz +Processing reads on 1 core in single-end mode ... === Summary === -Total reads processed: 99 -Reads with adapters: 52 (52.5%) -Reads written (passing filters): 99 (100.0%) +Total reads processed: 99 +Reads with adapters: 52 (52.5%) +Reads written (passing filters): 99 (100.0%) -Total basepairs processed: 24,849 bp -Quality-trimmed: 205 bp (0.8%) -Total written (filtered): 23,339 bp (93.9%) +Total basepairs processed: 24,849 bp +Quality-trimmed: 205 bp (0.8%) +Total written (filtered): 23,339 bp (93.9%) === Adapter 1 === -Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times +Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times. -Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-12 bp: 1 -Bases preceding removed adapters: - A: 9.6% - C: 38.5% - G: 23.1% - T: 28.8% - none/other: 0.0% - Overview of removed sequences length count expect max.err error counts 1 11 24.8 0 11 @@ -56,79 +44,71 @@ length count expect max.err error counts 13 2 0.0 1 2 14 1 0.0 1 1 16 1 0.0 1 1 -17 1 0.0 1 0 1 -20 2 0.0 1 2 -21 1 0.0 1 1 -24 1 0.0 1 1 -26 2 0.0 1 2 -31 1 0.0 1 1 -33 1 0.0 1 1 -41 2 0.0 1 2 -49 1 0.0 1 1 -50 1 0.0 1 1 -54 1 0.0 1 1 -56 1 0.0 1 1 -58 2 0.0 1 2 -60 1 0.0 1 1 -67 2 0.0 1 2 -68 1 0.0 1 1 -69 1 0.0 1 1 -73 1 0.0 1 1 -80 1 0.0 1 1 -86 1 0.0 1 1 +17 1 0.0 1 1 +20 2 0.0 2 2 +21 1 0.0 2 1 +24 1 0.0 2 1 +26 2 0.0 2 2 +31 1 0.0 3 1 +33 1 0.0 3 1 +41 2 0.0 4 2 +49 1 0.0 4 1 +50 1 0.0 5 1 +54 1 0.0 5 1 +56 1 0.0 5 1 +58 2 0.0 5 2 +60 1 0.0 6 1 +67 2 0.0 6 2 +68 1 0.0 6 1 +69 1 0.0 6 1 +73 1 0.0 7 1 +80 1 0.0 8 1 +86 1 0.0 8 1 + RUN STATISTICS FOR INPUT FILE: input_1.fastq.gz ============================================= 99 sequences processed in total - +Sequences removed because they became shorter than the length cutoff of 20 bp: 1 (1.0%) +Sequences removed because they were longer than the upper length cutoff: 0 (0.0%) +Sequences removed because of too many N bases: 0 (0.0%) SUMMARISING RUN PARAMETERS -========================== +========================= Input filename: input_2.fastq.gz Trimming mode: paired-end -Trim Galore version: 0.6.10 -Cutadapt version: 5.0 -Python version: 3.12.10 -Number of cores used for trimming: 4 +Trim Galore version: 2.2.0 (Oxidized Edition) Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 -Using Nextera adapter for trimming (count: 29). Second best hit was smallRNA (count: 0) -Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) -Maximum trimming error rate: 0.1 (default) +Adapter sequence: 'CTGTCTCTTATA' (user-specified or auto-detected) +Maximum trimming error rate: 0.1 Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp Output file will be GZIP compressed -This is cutadapt 5.0 with Python 3.12.10 -Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq.gz -Processing single-end reads on 4 cores ... +Trim Galore 2.2.0 (Oxidized Edition) — adapter trimming built in +This is cutadapt 4.0 (compatible; for MultiQC backwards compatibility) +Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq.gz +Processing reads on 1 core in single-end mode ... === Summary === -Total reads processed: 99 -Reads with adapters: 58 (58.6%) -Reads written (passing filters): 99 (100.0%) +Total reads processed: 99 +Reads with adapters: 58 (58.6%) +Reads written (passing filters): 99 (100.0%) -Total basepairs processed: 24,849 bp -Quality-trimmed: 745 bp (3.0%) -Total written (filtered): 23,035 bp (92.7%) +Total basepairs processed: 24,849 bp +Quality-trimmed: 745 bp (3.0%) +Total written (filtered): 23,035 bp (92.7%) === Adapter 1 === -Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times +Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times. -Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-12 bp: 1 -Bases preceding removed adapters: - A: 12.1% - C: 37.9% - G: 8.6% - T: 41.4% - none/other: 0.0% - Overview of removed sequences length count expect max.err error counts 1 16 24.8 0 16 @@ -144,29 +124,39 @@ length count expect max.err error counts 16 1 0.0 1 1 17 1 0.0 1 1 19 2 0.0 1 2 -21 1 0.0 1 1 -25 1 0.0 1 1 -30 1 0.0 1 1 -32 2 0.0 1 2 -34 1 0.0 1 1 -36 2 0.0 1 2 -38 1 0.0 1 1 -40 1 0.0 1 1 -41 1 0.0 1 1 -42 1 0.0 1 1 -43 1 0.0 1 1 -49 1 0.0 1 1 -51 1 0.0 1 1 -56 1 0.0 1 1 -57 1 0.0 1 1 -60 1 0.0 1 1 -67 1 0.0 1 1 -80 1 0.0 1 1 +21 1 0.0 2 1 +25 1 0.0 2 1 +30 1 0.0 3 1 +32 2 0.0 3 2 +34 1 0.0 3 1 +36 2 0.0 3 2 +38 1 0.0 3 1 +40 1 0.0 4 1 +41 1 0.0 4 1 +42 1 0.0 4 1 +43 1 0.0 4 1 +49 1 0.0 4 1 +51 1 0.0 5 1 +56 1 0.0 5 1 +57 1 0.0 5 1 +60 1 0.0 6 1 +67 1 0.0 6 1 +80 1 0.0 8 1 + RUN STATISTICS FOR INPUT FILE: input_2.fastq.gz ============================================= 99 sequences processed in total +Sequences removed because they became shorter than the length cutoff of 20 bp: 1 (1.0%) +Sequences removed because they were longer than the upper length cutoff: 0 (0.0%) +Sequences removed because of too many N bases: 0 (0.0%) + +=== Paired-end validation === + +Number of sequence pairs analysed: 99 -Total number of sequences analysed for the sequence pair length validation: 99 +Number of sequence pairs removed because at least one read was shorter than the length cutoff (1.01%): + 1 +Number of pairs removed for N content (0.00%): + 0 -Number of sequence pairs removed because at least one read was shorter than the length cutoff (20 bp): 1 (1.01%) diff --git a/tools/trim_galore/test-data/paired_hardtrimmed3_pair1_.fastq.gz b/tools/trim_galore/test-data/paired_hardtrimmed3_pair1_.fastq.gz new file mode 100644 index 0000000000..01f07eb87e Binary files /dev/null and b/tools/trim_galore/test-data/paired_hardtrimmed3_pair1_.fastq.gz differ diff --git a/tools/trim_galore/test-data/paired_hardtrimmed3_pair1_.fastqsanger b/tools/trim_galore/test-data/paired_hardtrimmed3_pair1_.fastqsanger deleted file mode 100644 index c0ee2935c2..0000000000 --- a/tools/trim_galore/test-data/paired_hardtrimmed3_pair1_.fastqsanger +++ /dev/null @@ -1,396 +0,0 @@ -@M01368:8:000000000-A3GHV:1:1101:6911:8255/1 -TCGCACCTACGTTCAATATT -+ -FFEFE;DFFFDFBFF/9BFB -@M01368:8:000000000-A3GHV:1:1101:14518:9998/1 -GACCTGTGATCCATCGTGAT -+ -FF//;BFFFFFBF/9D:A// -@M01368:8:000000000-A3GHV:1:1101:18422:19051/1 -TATTATTTATCGCACCTACG -+ -FFEFBFFFFFFFFFF:FFF. -@M01368:8:000000000-A3GHV:1:1101:25545:21098/1 -TTTAAGGGGAACGGGTGGGG -+ -FBFFFFFFBF..A..;@B-- -@M01368:8:000000000-A3GHV:1:1101:5446:12248/1 -AAGGGGAACGTGTGGGCTAT -+ -FFFBFFFFFFEFFFFFEFFF -@M01368:8:000000000-A3GHV:1:1101:5861:6452/1 -TGATCCATCGTGATGTCTTT -+ -FFFFFBFFBD.AFFFFFFF0 -@M01368:8:000000000-A3GHV:1:1102:10403:6021/1 -ACCCTATGTCGCAGTATCTG -+ -..9..9/9;BEF;A:9/BFF -@M01368:8:000000000-A3GHV:1:1102:10677:23253/1 -AATTCTGTCTCTTATACACA -+ -F0FFFFFFFFFFFFFFFFF: -@M01368:8:000000000-A3GHV:1:1102:13809:1733/1 -GTCTCTTATACACATCTCCG -+ -FBBBFFFFFBFF0BFBF9F. -@M01368:8:000000000-A3GHV:1:1102:17584:10050/1 -AATCTCGTATGCCGTCTTCT -+ -FF;.BF9ADF/9;A;DFFF0 -@M01368:8:000000000-A3GHV:1:1102:18842:24844/1 -TCTGTCTTTGATTCCTGCCT -+ -BBBFBFBFFF//BFB/BFF/ -@M01368:8:000000000-A3GHV:1:1102:21788:11027/1 -ACTAAAGTGTGTTAATTCTG -+ -FBFF0FFBBBBFBFBFFFF0 -@M01368:8:000000000-A3GHV:1:1102:5728:8777/1 -CCTGAAGTAGGAACCAGATG -+ -FFFFBBFFFFF/FFEFFFBB -@M01368:8:000000000-A3GHV:1:1102:5983:21093/1 -TATTATTTATCGCACCTACG -+ -F0BFFFBBFF0EFFFFFFFA -@M01368:8:000000000-A3GHV:1:1102:7765:18353/1 -CCTATGTCGCAGTATCTGTC -+ -.::FBFBFFE-A9:BFFFF0 -@M01368:8:000000000-A3GHV:1:1103:10405:17879/1 -TATTATTTATCGCACCTACG -+ -FBFFFFFFFFFFFFFFFFF? -@M01368:8:000000000-A3GHV:1:1103:13493:8205/1 -GATTCCTGCCTCATCCTATT -+ -FFFFFFFFBFBBBFFFFEFF -@M01368:8:000000000-A3GHV:1:1103:14060:21273/1 -TATTACTGTCTCTTATACAC -+ -:FFFBB/BFFFFFBFFFFB/ -@M01368:8:000000000-A3GHV:1:1103:14528:12993/1 -TCGCAGTATCTGTCTTTGAT -+ -BFEA-A.B//9F9F//;BBB -@M01368:8:000000000-A3GHV:1:1103:17657:20856/1 -TGCCTCATCCTATTATTTAT -+ -FFFFFFFFFFF/FFFFBFF9 -@M01368:8:000000000-A3GHV:1:1103:23099:3794/1 -GCGAATCTCGGATGCCGTCT -+ -FFFF@;.9BF..;-./-A=F -@M01368:8:000000000-A3GHV:1:1103:25465:20010/1 -AAAAAAAAAAAACAACAGAC -+ -.B/;:>---;--..;.../9 -@M01368:8:000000000-A3GHV:1:1103:25519:6301/1 -GAGACTAAGGCGAATTTCGT -+ --B?DFFF/B/B?---.//;. -@M01368:8:000000000-A3GHV:1:1103:6074:6549/1 -GTATCTGTCTTTGATTCCTG -+ -A./FFFFFFFFFFFF/BFF/ -@M01368:8:000000000-A3GHV:1:1104:11572:8264/1 -TATTATTTATCGCACCTACG -+ -B/BFFFF/BFFE?EFAFEF9 -@M01368:8:000000000-A3GHV:1:1104:11928:24197/1 -TAGGAACCAGATGTCGGATA -+ -FFFFFFFFFFFFFFFFF@D9 -@M01368:8:000000000-A3GHV:1:1104:12288:20635/1 -TCTGTCTTTGATTCCTGCCT -+ -;FBBFBFB///;99999BA9 -@M01368:8:000000000-A3GHV:1:1104:13832:4398/1 -ACCCAGTAGACTAGTGCGTC -+ -9...:.9//9////;/9-.- -@M01368:8:000000000-A3GHV:1:1104:23670:4161/1 -GGGTGATAGACCTGTGATCC -+ -BF./AAF/BFFBBF/F/BFB -@M01368:8:000000000-A3GHV:1:1104:24927:22253/1 -TTCCTGCCTCATCCTATTAT -+ -//;/;9/A/;/9BFFFBFBB -@M01368:8:000000000-A3GHV:1:1104:4705:7727/1 -CGTGATGTCTTATTTAAGGG -+ -F?.AFFF/BFFEFFFFFEFF -@M01368:8:000000000-A3GHV:1:1104:7756:4776/1 -GTCGCAGTATCTGTCTTTGA -+ -FFBEFFF:9FFFFBBFFFF/ -@M01368:8:000000000-A3GHV:1:1105:11310:21761/1 -TGTCGGATACAGTTCACTTT -+ -FFBFFFFFFFFFFFFEFFF0 -@M01368:8:000000000-A3GHV:1:1105:11578:14676/1 -TGAAGTAGGAACCAGATGTC -+ -FF/FFFFFFFFFFFFFFFF0 -@M01368:8:000000000-A3GHV:1:1105:13575:19255/1 -TGGGCTATTTAGGTTTTATG -+ -FF?B---;B99//9/;A//9 -@M01368:8:000000000-A3GHV:1:1105:15502:11453/1 -CTGTCTCTTATACACATCTC -+ -FFFFEFFFFFFFFBFFFBFF -@M01368:8:000000000-A3GHV:1:1105:16787:9771/1 -GACCTGTGATCCATCGTGAT -+ -FFFFFFBFFFFFFFFFEFF/ -@M01368:8:000000000-A3GHV:1:1105:17564:15723/1 -TTAGGTTTTATGACCCTGAA -+ -FFBBFBBFFFFFE1:BFFB0 -@M01368:8:000000000-A3GHV:1:1105:18108:12091/1 -GATACCTGTCTCTTATACAC -+ -F-A.B//;BFFEF//BB/// -@M01368:8:000000000-A3GHV:1:1105:18331:16683/1 -CGCACCTACGTTCAATATTA -+ -B.DABFFFFFFB.//:FB// -@M01368:8:000000000-A3GHV:1:1105:20577:18421/1 -CTTTGATTCCTGCCTCATCC -+ -FBB/:FF9BF/BF.BBFF/: -@M01368:8:000000000-A3GHV:1:1105:21099:2895/1 -ATTCCTGCCTCATCCTATTT -+ -BFBFFBFF.0:000BFFBF0 -@M01368:8:000000000-A3GHV:1:1105:26469:19245/1 -ACCTGTGATCCATCGTGATG -+ -FFFFFFF/9FFFFBDEFFFF -@M01368:8:000000000-A3GHV:1:1105:7169:17145/1 -GAACATACTTACTAAAGTGT -+ --9@DDBFFFFFFFFBBFFF0 -@M01368:8:000000000-A3GHV:1:1106:15481:16176/1 -TGTGGGCTATTTAGGTTTTT -+ -FE:AAFEF.BFFFFF;9FF. -@M01368:8:000000000-A3GHV:1:1106:17389:18671/1 -GCAGTATCTGGCTTTGATTC -+ -..;A;/B/;B//;;//BBF/ -@M01368:8:000000000-A3GHV:1:1106:22927:3863/1 -CTCCGAGCCCACGAGACTAA -+ -//FFF-=--.99=--.9/// -@M01368:8:000000000-A3GHV:1:1106:23625:4236/1 -ATTCCTGCCTCATCCTATTA -+ -EFFFFFFFAF///9FF/BB/ -@M01368:8:000000000-A3GHV:1:1106:26031:14826/1 -GTATGCCGTCTTCTGCTTGA -+ -AFFFFFF;;BFE/BFFFFB: -@M01368:8:000000000-A3GHV:1:1106:27607:15990/1 -AACTACAACCCACGGACGCA -+ -;B.//////;...---9--- -@M01368:8:000000000-A3GHV:1:1106:6455:17124/1 -TATTATTTATCGCACCTACG -+ -F/FFBFFFFFFADE>BFFF9 -@M01368:8:000000000-A3GHV:1:1107:14451:26748/1 -TTTAGGTTTTATGACCCTGA -+ -FBFFFF;FFEBFBFFB./9. -@M01368:8:000000000-A3GHV:1:1107:18714:24788/1 -TTTAGGTTTTATGACCCTGA -+ -FFFFFFBFFFFFBBFFBBF/ -@M01368:8:000000000-A3GHV:1:1107:21355:8333/1 -CCTGAAGTAGGAACCAGATG -+ -FFFFFFFFFFFFBFF.AA/9 -@M01368:8:000000000-A3GHV:1:1107:22864:15712/1 -ACTAAGGCGAATCTCGTATG -+ -9DFFF/FFBF;-.:;.9A// -@M01368:8:000000000-A3GHV:1:1107:24817:14233/1 -TCAATATTACAGGCGAACAT -+ -FFFFFBF//:BFBACF--9/ -@M01368:8:000000000-A3GHV:1:1107:24830:14440/1 -ATCCTATTATTTATCGCACC -+ -FFFFFFFFFFF/BFBEFFC- -@M01368:8:000000000-A3GHV:1:1107:26478:23602/1 -CTGTCTCTTATACACCTCTC -+ -BFFFBBFFF//B/////-;9 -@M01368:8:000000000-A3GHV:1:1108:11059:23181/1 -GACCTGTGATCCATCGTGAT -+ -FFFFBF;BBFFBFFFFE?F/ -@M01368:8:000000000-A3GHV:1:1108:12233:13498/1 -GAACCCTGTCTCTTATACAC -+ -EFF?EFFFF0BFFFFBBB00 -@M01368:8:000000000-A3GHV:1:1108:14328:14751/1 -GGATACCTGTCTCTTATACA -+ -EFFFBF/BF/FFEFFBFFF/ -@M01368:8:000000000-A3GHV:1:1108:16350:22621/1 -TGGGCTATTTAGGTTTTATG -+ -BE.99FAFFFFFFF/;EFF/ -@M01368:8:000000000-A3GHV:1:1108:21791:23272/1 -TGTGGGCTATTTAGGTTTTA -+ -EEFEFFFBFBFBBFFFFF./ -@M01368:8:000000000-A3GHV:1:1108:23314:26089/1 -TGAAGTAGGAACCAGATGTG -+ -BB./:BFFF/F;AA?F//// -@M01368:8:000000000-A3GHV:1:1108:25084:18127/1 -TGTGATCCATCGTGATGTCT -+ -FF9FBFFB/F/.;FFFF/BB -@M01368:8:000000000-A3GHV:1:1108:7626:10048/1 -TATGCCGTCTTCTGCTTGAA -+ -AAFFFFFFAFABBFFFB/;; -@M01368:8:000000000-A3GHV:1:1109:11270:12255/1 -AGACTAAGGCGAATCTCGTT -+ --@DBFFFFBFD---.;BAA. -@M01368:8:000000000-A3GHV:1:1109:19224:11655/1 -CTTATACACATCTCCGAGCC -+ -FFB/;F/99/FBFFBDCF;B -@M01368:8:000000000-A3GHV:1:1109:21978:9490/1 -GGCGAATCTCGTATGCAGTT -+ -FFFFA;;;FF..9//;/;;/ -@M01368:8:000000000-A3GHV:1:1109:22910:4079/1 -GATACAGTCCACTTTAGCTA -+ -CCDFFFFFFF0BFFFFFFF0 -@M01368:8:000000000-A3GHV:1:1109:24217:19077/1 -TGCCTCATCCTATTACTGTC -+ -9;F./B//BBFB9////99/ -@M01368:8:000000000-A3GHV:1:1109:25237:11722/1 -AGGCGAATATCGTATGCCGT -+ -BF/99D-;.//.../9;B-. -@M01368:8:000000000-A3GHV:1:1109:27103:7153/1 -GACCCTGAAGTAGCTGTCTC -+ -BBFFBFFFF///;/9B;/// -@M01368:8:000000000-A3GHV:1:1109:8763:7859/1 -GGGCTATTTAGGCTTTATGC -+ -EDAA.A/BFFFFFFFFFEB/ -@M01368:8:000000000-A3GHV:1:1110:12808:18367/1 -AAGGGGAACGTGTGGGCCTG -+ -BFBFFB9..AF?FFFFFFF9 -@M01368:8:000000000-A3GHV:1:1110:14751:26540/1 -GTATCTGTCTTTGATTCCTG -+ -9-/;B9BF/9BB//B/;/// -@M01368:8:000000000-A3GHV:1:1110:15519:10008/1 -ATGACCCTGAAGTAGGAACC -+ -FFFFFBFFF.BFFFBFBBA; -@M01368:8:000000000-A3GHV:1:1110:16559:7507/1 -AGGGTGATAGACCTGTGATC -+ -/99;;:.//:FFFFBBF//9 -@M01368:8:000000000-A3GHV:1:1110:16700:6843/1 -CTTATACACATCTCCGAGCC -+ -BFFFFB/BFFBFFF/ADCAD -@M01368:8:000000000-A3GHV:1:1110:18014:7856/1 -TGCTTGCAAAAAAAAAATAG -+ -/BBFFF.9/FF?DA=@9./9 -@M01368:8:000000000-A3GHV:1:1110:23303:11846/1 -CTGTCTTTGATTCCTGCCTC -+ -FBBFFFF/BBFFFFBFF9B; -@M01368:8:000000000-A3GHV:1:1111:13195:14562/1 -ACATCTCCGAGCCCACGAGA -+ -FFEFFFFED-DCFF?.:BFA -@M01368:8:000000000-A3GHV:1:1111:14287:20817/1 -ATTAAAGTGTGTTAATTAAT -+ -B//B/;/;9//9//;B/:B9 -@M01368:8:000000000-A3GHV:1:1111:23624:14516/1 -CATCGTGATGTCTTATTTAG -+ -B/FBDE9/;99/B/BFFEF/ -@M01368:8:000000000-A3GHV:1:1111:7437:24508/1 -CACATCTCCGAGCCCACGAG -+ -FFFFB:FFFFD=CAEEEF>- -@M01368:8:000000000-A3GHV:1:1111:8665:25542/1 -TACGTTCAATATTACAGGCG -+ -FFFBA/F//BFFFBF/;/A- -@M01368:8:000000000-A3GHV:1:1111:9655:13916/1 -GTCGCAGTATCTGTCTTTGA -+ -F/B?A@F-9BBFF//BB/9/ -@M01368:8:000000000-A3GHV:1:1112:10272:7828/1 -CGAATCTCGTATGCCGTCTT -+ -FAD--.99;A?////:.9A. -@M01368:8:000000000-A3GHV:1:1112:9832:16531/1 -ATGACCTTGAAGTAGGAACC -+ -FFF/BF//9/B//9:B//.. -@M01368:8:000000000-A3GHV:1:1112:9832:6701/1 -TTTATGACCCTGAAGTAGGA -+ -FFFFFFF/B./FB///;BF/ -@M01368:8:000000000-A3GHV:1:1113:13532:8618/1 -AGCTACCTCCAAGTGTTATG -+ -FF/BFFB/:.BB//;FBFFF -@M01368:8:000000000-A3GHV:1:1113:21679:18011/1 -AGACTAAGGCGAAGTTCATT -+ -;-;AFFE;/99-9--/;/// -@M01368:8:000000000-A3GHV:1:1113:25528:14016/1 -TCGTGATGTCTTATTTAAGG -+ -FFFEABF//99FFEFFFBFF -@M01368:8:000000000-A3GHV:1:1113:5741:16959/1 -GGGCTATTTAGGTTTTATGA -+ -9AD;.FFFB/B/;FBA/B// -@M01368:8:000000000-A3GHV:1:1114:10130:11959/1 -GCAACAAACACACATCCAGA -+ -F./B/..9.9:...////// -@M01368:8:000000000-A3GHV:1:1114:14540:5315/1 -TGGGGGCTATTTAGGTTTTT -+ -;A.-=A--:BBFB90;;FE. -@M01368:8:000000000-A3GHV:1:1114:15066:16302/1 -TAATTATTGCTTGTAGGACA -+ -F/;BFF/;/////9/////. -@M01368:8:000000000-A3GHV:1:1114:16639:15258/1 -ACATACTTACTAAATTGTGT -+ --9-99/B9F/://///99/; -@M01368:8:000000000-A3GHV:1:1114:2404:13066/1 -ATCTGTCTATTATACACATC -+ -FFFFFFFFFFFFFFFFEFF0 diff --git a/tools/trim_galore/test-data/paired_hardtrimmed3_pair2_.fastq.gz b/tools/trim_galore/test-data/paired_hardtrimmed3_pair2_.fastq.gz new file mode 100644 index 0000000000..68acfdf8b0 Binary files /dev/null and b/tools/trim_galore/test-data/paired_hardtrimmed3_pair2_.fastq.gz differ diff --git a/tools/trim_galore/test-data/paired_hardtrimmed3_pair2_.fastqsanger b/tools/trim_galore/test-data/paired_hardtrimmed3_pair2_.fastqsanger deleted file mode 100644 index c0ee2935c2..0000000000 --- a/tools/trim_galore/test-data/paired_hardtrimmed3_pair2_.fastqsanger +++ /dev/null @@ -1,396 +0,0 @@ -@M01368:8:000000000-A3GHV:1:1101:6911:8255/1 -TCGCACCTACGTTCAATATT -+ -FFEFE;DFFFDFBFF/9BFB -@M01368:8:000000000-A3GHV:1:1101:14518:9998/1 -GACCTGTGATCCATCGTGAT -+ -FF//;BFFFFFBF/9D:A// -@M01368:8:000000000-A3GHV:1:1101:18422:19051/1 -TATTATTTATCGCACCTACG -+ -FFEFBFFFFFFFFFF:FFF. -@M01368:8:000000000-A3GHV:1:1101:25545:21098/1 -TTTAAGGGGAACGGGTGGGG -+ -FBFFFFFFBF..A..;@B-- -@M01368:8:000000000-A3GHV:1:1101:5446:12248/1 -AAGGGGAACGTGTGGGCTAT -+ -FFFBFFFFFFEFFFFFEFFF -@M01368:8:000000000-A3GHV:1:1101:5861:6452/1 -TGATCCATCGTGATGTCTTT -+ -FFFFFBFFBD.AFFFFFFF0 -@M01368:8:000000000-A3GHV:1:1102:10403:6021/1 -ACCCTATGTCGCAGTATCTG -+ -..9..9/9;BEF;A:9/BFF -@M01368:8:000000000-A3GHV:1:1102:10677:23253/1 -AATTCTGTCTCTTATACACA -+ -F0FFFFFFFFFFFFFFFFF: -@M01368:8:000000000-A3GHV:1:1102:13809:1733/1 -GTCTCTTATACACATCTCCG -+ -FBBBFFFFFBFF0BFBF9F. -@M01368:8:000000000-A3GHV:1:1102:17584:10050/1 -AATCTCGTATGCCGTCTTCT -+ -FF;.BF9ADF/9;A;DFFF0 -@M01368:8:000000000-A3GHV:1:1102:18842:24844/1 -TCTGTCTTTGATTCCTGCCT -+ -BBBFBFBFFF//BFB/BFF/ -@M01368:8:000000000-A3GHV:1:1102:21788:11027/1 -ACTAAAGTGTGTTAATTCTG -+ -FBFF0FFBBBBFBFBFFFF0 -@M01368:8:000000000-A3GHV:1:1102:5728:8777/1 -CCTGAAGTAGGAACCAGATG -+ -FFFFBBFFFFF/FFEFFFBB -@M01368:8:000000000-A3GHV:1:1102:5983:21093/1 -TATTATTTATCGCACCTACG -+ -F0BFFFBBFF0EFFFFFFFA -@M01368:8:000000000-A3GHV:1:1102:7765:18353/1 -CCTATGTCGCAGTATCTGTC -+ -.::FBFBFFE-A9:BFFFF0 -@M01368:8:000000000-A3GHV:1:1103:10405:17879/1 -TATTATTTATCGCACCTACG -+ -FBFFFFFFFFFFFFFFFFF? -@M01368:8:000000000-A3GHV:1:1103:13493:8205/1 -GATTCCTGCCTCATCCTATT -+ -FFFFFFFFBFBBBFFFFEFF -@M01368:8:000000000-A3GHV:1:1103:14060:21273/1 -TATTACTGTCTCTTATACAC -+ -:FFFBB/BFFFFFBFFFFB/ -@M01368:8:000000000-A3GHV:1:1103:14528:12993/1 -TCGCAGTATCTGTCTTTGAT -+ -BFEA-A.B//9F9F//;BBB -@M01368:8:000000000-A3GHV:1:1103:17657:20856/1 -TGCCTCATCCTATTATTTAT -+ -FFFFFFFFFFF/FFFFBFF9 -@M01368:8:000000000-A3GHV:1:1103:23099:3794/1 -GCGAATCTCGGATGCCGTCT -+ -FFFF@;.9BF..;-./-A=F -@M01368:8:000000000-A3GHV:1:1103:25465:20010/1 -AAAAAAAAAAAACAACAGAC -+ -.B/;:>---;--..;.../9 -@M01368:8:000000000-A3GHV:1:1103:25519:6301/1 -GAGACTAAGGCGAATTTCGT -+ --B?DFFF/B/B?---.//;. -@M01368:8:000000000-A3GHV:1:1103:6074:6549/1 -GTATCTGTCTTTGATTCCTG -+ -A./FFFFFFFFFFFF/BFF/ -@M01368:8:000000000-A3GHV:1:1104:11572:8264/1 -TATTATTTATCGCACCTACG -+ -B/BFFFF/BFFE?EFAFEF9 -@M01368:8:000000000-A3GHV:1:1104:11928:24197/1 -TAGGAACCAGATGTCGGATA -+ -FFFFFFFFFFFFFFFFF@D9 -@M01368:8:000000000-A3GHV:1:1104:12288:20635/1 -TCTGTCTTTGATTCCTGCCT -+ -;FBBFBFB///;99999BA9 -@M01368:8:000000000-A3GHV:1:1104:13832:4398/1 -ACCCAGTAGACTAGTGCGTC -+ -9...:.9//9////;/9-.- -@M01368:8:000000000-A3GHV:1:1104:23670:4161/1 -GGGTGATAGACCTGTGATCC -+ -BF./AAF/BFFBBF/F/BFB -@M01368:8:000000000-A3GHV:1:1104:24927:22253/1 -TTCCTGCCTCATCCTATTAT -+ -//;/;9/A/;/9BFFFBFBB -@M01368:8:000000000-A3GHV:1:1104:4705:7727/1 -CGTGATGTCTTATTTAAGGG -+ -F?.AFFF/BFFEFFFFFEFF -@M01368:8:000000000-A3GHV:1:1104:7756:4776/1 -GTCGCAGTATCTGTCTTTGA -+ -FFBEFFF:9FFFFBBFFFF/ -@M01368:8:000000000-A3GHV:1:1105:11310:21761/1 -TGTCGGATACAGTTCACTTT -+ -FFBFFFFFFFFFFFFEFFF0 -@M01368:8:000000000-A3GHV:1:1105:11578:14676/1 -TGAAGTAGGAACCAGATGTC -+ -FF/FFFFFFFFFFFFFFFF0 -@M01368:8:000000000-A3GHV:1:1105:13575:19255/1 -TGGGCTATTTAGGTTTTATG -+ -FF?B---;B99//9/;A//9 -@M01368:8:000000000-A3GHV:1:1105:15502:11453/1 -CTGTCTCTTATACACATCTC -+ -FFFFEFFFFFFFFBFFFBFF -@M01368:8:000000000-A3GHV:1:1105:16787:9771/1 -GACCTGTGATCCATCGTGAT -+ -FFFFFFBFFFFFFFFFEFF/ -@M01368:8:000000000-A3GHV:1:1105:17564:15723/1 -TTAGGTTTTATGACCCTGAA -+ -FFBBFBBFFFFFE1:BFFB0 -@M01368:8:000000000-A3GHV:1:1105:18108:12091/1 -GATACCTGTCTCTTATACAC -+ -F-A.B//;BFFEF//BB/// -@M01368:8:000000000-A3GHV:1:1105:18331:16683/1 -CGCACCTACGTTCAATATTA -+ -B.DABFFFFFFB.//:FB// -@M01368:8:000000000-A3GHV:1:1105:20577:18421/1 -CTTTGATTCCTGCCTCATCC -+ -FBB/:FF9BF/BF.BBFF/: -@M01368:8:000000000-A3GHV:1:1105:21099:2895/1 -ATTCCTGCCTCATCCTATTT -+ -BFBFFBFF.0:000BFFBF0 -@M01368:8:000000000-A3GHV:1:1105:26469:19245/1 -ACCTGTGATCCATCGTGATG -+ -FFFFFFF/9FFFFBDEFFFF -@M01368:8:000000000-A3GHV:1:1105:7169:17145/1 -GAACATACTTACTAAAGTGT -+ --9@DDBFFFFFFFFBBFFF0 -@M01368:8:000000000-A3GHV:1:1106:15481:16176/1 -TGTGGGCTATTTAGGTTTTT -+ -FE:AAFEF.BFFFFF;9FF. -@M01368:8:000000000-A3GHV:1:1106:17389:18671/1 -GCAGTATCTGGCTTTGATTC -+ -..;A;/B/;B//;;//BBF/ -@M01368:8:000000000-A3GHV:1:1106:22927:3863/1 -CTCCGAGCCCACGAGACTAA -+ -//FFF-=--.99=--.9/// -@M01368:8:000000000-A3GHV:1:1106:23625:4236/1 -ATTCCTGCCTCATCCTATTA -+ -EFFFFFFFAF///9FF/BB/ -@M01368:8:000000000-A3GHV:1:1106:26031:14826/1 -GTATGCCGTCTTCTGCTTGA -+ -AFFFFFF;;BFE/BFFFFB: -@M01368:8:000000000-A3GHV:1:1106:27607:15990/1 -AACTACAACCCACGGACGCA -+ -;B.//////;...---9--- -@M01368:8:000000000-A3GHV:1:1106:6455:17124/1 -TATTATTTATCGCACCTACG -+ -F/FFBFFFFFFADE>BFFF9 -@M01368:8:000000000-A3GHV:1:1107:14451:26748/1 -TTTAGGTTTTATGACCCTGA -+ -FBFFFF;FFEBFBFFB./9. -@M01368:8:000000000-A3GHV:1:1107:18714:24788/1 -TTTAGGTTTTATGACCCTGA -+ -FFFFFFBFFFFFBBFFBBF/ -@M01368:8:000000000-A3GHV:1:1107:21355:8333/1 -CCTGAAGTAGGAACCAGATG -+ -FFFFFFFFFFFFBFF.AA/9 -@M01368:8:000000000-A3GHV:1:1107:22864:15712/1 -ACTAAGGCGAATCTCGTATG -+ -9DFFF/FFBF;-.:;.9A// -@M01368:8:000000000-A3GHV:1:1107:24817:14233/1 -TCAATATTACAGGCGAACAT -+ -FFFFFBF//:BFBACF--9/ -@M01368:8:000000000-A3GHV:1:1107:24830:14440/1 -ATCCTATTATTTATCGCACC -+ -FFFFFFFFFFF/BFBEFFC- -@M01368:8:000000000-A3GHV:1:1107:26478:23602/1 -CTGTCTCTTATACACCTCTC -+ -BFFFBBFFF//B/////-;9 -@M01368:8:000000000-A3GHV:1:1108:11059:23181/1 -GACCTGTGATCCATCGTGAT -+ -FFFFBF;BBFFBFFFFE?F/ -@M01368:8:000000000-A3GHV:1:1108:12233:13498/1 -GAACCCTGTCTCTTATACAC -+ -EFF?EFFFF0BFFFFBBB00 -@M01368:8:000000000-A3GHV:1:1108:14328:14751/1 -GGATACCTGTCTCTTATACA -+ -EFFFBF/BF/FFEFFBFFF/ -@M01368:8:000000000-A3GHV:1:1108:16350:22621/1 -TGGGCTATTTAGGTTTTATG -+ -BE.99FAFFFFFFF/;EFF/ -@M01368:8:000000000-A3GHV:1:1108:21791:23272/1 -TGTGGGCTATTTAGGTTTTA -+ -EEFEFFFBFBFBBFFFFF./ -@M01368:8:000000000-A3GHV:1:1108:23314:26089/1 -TGAAGTAGGAACCAGATGTG -+ -BB./:BFFF/F;AA?F//// -@M01368:8:000000000-A3GHV:1:1108:25084:18127/1 -TGTGATCCATCGTGATGTCT -+ -FF9FBFFB/F/.;FFFF/BB -@M01368:8:000000000-A3GHV:1:1108:7626:10048/1 -TATGCCGTCTTCTGCTTGAA -+ -AAFFFFFFAFABBFFFB/;; -@M01368:8:000000000-A3GHV:1:1109:11270:12255/1 -AGACTAAGGCGAATCTCGTT -+ --@DBFFFFBFD---.;BAA. -@M01368:8:000000000-A3GHV:1:1109:19224:11655/1 -CTTATACACATCTCCGAGCC -+ -FFB/;F/99/FBFFBDCF;B -@M01368:8:000000000-A3GHV:1:1109:21978:9490/1 -GGCGAATCTCGTATGCAGTT -+ -FFFFA;;;FF..9//;/;;/ -@M01368:8:000000000-A3GHV:1:1109:22910:4079/1 -GATACAGTCCACTTTAGCTA -+ -CCDFFFFFFF0BFFFFFFF0 -@M01368:8:000000000-A3GHV:1:1109:24217:19077/1 -TGCCTCATCCTATTACTGTC -+ -9;F./B//BBFB9////99/ -@M01368:8:000000000-A3GHV:1:1109:25237:11722/1 -AGGCGAATATCGTATGCCGT -+ -BF/99D-;.//.../9;B-. -@M01368:8:000000000-A3GHV:1:1109:27103:7153/1 -GACCCTGAAGTAGCTGTCTC -+ -BBFFBFFFF///;/9B;/// -@M01368:8:000000000-A3GHV:1:1109:8763:7859/1 -GGGCTATTTAGGCTTTATGC -+ -EDAA.A/BFFFFFFFFFEB/ -@M01368:8:000000000-A3GHV:1:1110:12808:18367/1 -AAGGGGAACGTGTGGGCCTG -+ -BFBFFB9..AF?FFFFFFF9 -@M01368:8:000000000-A3GHV:1:1110:14751:26540/1 -GTATCTGTCTTTGATTCCTG -+ -9-/;B9BF/9BB//B/;/// -@M01368:8:000000000-A3GHV:1:1110:15519:10008/1 -ATGACCCTGAAGTAGGAACC -+ -FFFFFBFFF.BFFFBFBBA; -@M01368:8:000000000-A3GHV:1:1110:16559:7507/1 -AGGGTGATAGACCTGTGATC -+ -/99;;:.//:FFFFBBF//9 -@M01368:8:000000000-A3GHV:1:1110:16700:6843/1 -CTTATACACATCTCCGAGCC -+ -BFFFFB/BFFBFFF/ADCAD -@M01368:8:000000000-A3GHV:1:1110:18014:7856/1 -TGCTTGCAAAAAAAAAATAG -+ -/BBFFF.9/FF?DA=@9./9 -@M01368:8:000000000-A3GHV:1:1110:23303:11846/1 -CTGTCTTTGATTCCTGCCTC -+ -FBBFFFF/BBFFFFBFF9B; -@M01368:8:000000000-A3GHV:1:1111:13195:14562/1 -ACATCTCCGAGCCCACGAGA -+ -FFEFFFFED-DCFF?.:BFA -@M01368:8:000000000-A3GHV:1:1111:14287:20817/1 -ATTAAAGTGTGTTAATTAAT -+ -B//B/;/;9//9//;B/:B9 -@M01368:8:000000000-A3GHV:1:1111:23624:14516/1 -CATCGTGATGTCTTATTTAG -+ -B/FBDE9/;99/B/BFFEF/ -@M01368:8:000000000-A3GHV:1:1111:7437:24508/1 -CACATCTCCGAGCCCACGAG -+ -FFFFB:FFFFD=CAEEEF>- -@M01368:8:000000000-A3GHV:1:1111:8665:25542/1 -TACGTTCAATATTACAGGCG -+ -FFFBA/F//BFFFBF/;/A- -@M01368:8:000000000-A3GHV:1:1111:9655:13916/1 -GTCGCAGTATCTGTCTTTGA -+ -F/B?A@F-9BBFF//BB/9/ -@M01368:8:000000000-A3GHV:1:1112:10272:7828/1 -CGAATCTCGTATGCCGTCTT -+ -FAD--.99;A?////:.9A. -@M01368:8:000000000-A3GHV:1:1112:9832:16531/1 -ATGACCTTGAAGTAGGAACC -+ -FFF/BF//9/B//9:B//.. -@M01368:8:000000000-A3GHV:1:1112:9832:6701/1 -TTTATGACCCTGAAGTAGGA -+ -FFFFFFF/B./FB///;BF/ -@M01368:8:000000000-A3GHV:1:1113:13532:8618/1 -AGCTACCTCCAAGTGTTATG -+ -FF/BFFB/:.BB//;FBFFF -@M01368:8:000000000-A3GHV:1:1113:21679:18011/1 -AGACTAAGGCGAAGTTCATT -+ -;-;AFFE;/99-9--/;/// -@M01368:8:000000000-A3GHV:1:1113:25528:14016/1 -TCGTGATGTCTTATTTAAGG -+ -FFFEABF//99FFEFFFBFF -@M01368:8:000000000-A3GHV:1:1113:5741:16959/1 -GGGCTATTTAGGTTTTATGA -+ -9AD;.FFFB/B/;FBA/B// -@M01368:8:000000000-A3GHV:1:1114:10130:11959/1 -GCAACAAACACACATCCAGA -+ -F./B/..9.9:...////// -@M01368:8:000000000-A3GHV:1:1114:14540:5315/1 -TGGGGGCTATTTAGGTTTTT -+ -;A.-=A--:BBFB90;;FE. -@M01368:8:000000000-A3GHV:1:1114:15066:16302/1 -TAATTATTGCTTGTAGGACA -+ -F/;BFF/;/////9/////. -@M01368:8:000000000-A3GHV:1:1114:16639:15258/1 -ACATACTTACTAAATTGTGT -+ --9-99/B9F/://///99/; -@M01368:8:000000000-A3GHV:1:1114:2404:13066/1 -ATCTGTCTATTATACACATC -+ -FFFFFFFFFFFFFFFFEFF0 diff --git a/tools/trim_galore/test-data/paired_hardtrimmed5_pair1_.fastq.gz b/tools/trim_galore/test-data/paired_hardtrimmed5_pair1_.fastq.gz new file mode 100644 index 0000000000..6d12db1c5a Binary files /dev/null and b/tools/trim_galore/test-data/paired_hardtrimmed5_pair1_.fastq.gz differ diff --git a/tools/trim_galore/test-data/paired_hardtrimmed5_pair1_.fastqsanger b/tools/trim_galore/test-data/paired_hardtrimmed5_pair1_.fastqsanger deleted file mode 100644 index b1d8bc7b90..0000000000 --- a/tools/trim_galore/test-data/paired_hardtrimmed5_pair1_.fastqsanger +++ /dev/null @@ -1,396 +0,0 @@ -@M01368:8:000000000-A3GHV:1:1101:6911:8255/1 -ATCTGGTTCCTACTTCAGGG -+ -BCCCCFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1101:14518:9998/1 -GTTATTATTATGTCCTACAA -+ -AAAAAFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1101:18422:19051/1 -GTATCCGACATCTGGTTCCT -+ -CCCCCFDDDDDFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1101:25545:21098/1 -ATTAATTAACACACTTTAGT -+ -33AA?DFD5BDFGGGFEBDG -@M01368:8:000000000-A3GHV:1:1101:5446:12248/1 -AATTAACACACTTTAGTAAG -+ -CCCCDFFFFCCFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1101:5861:6452/1 -ATTATGTCCTACAAGCATTA -+ -ABCCCFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1102:10403:6021/1 -CGCTCCGGGCCCATAACACT -+ ->A@AAAAD2ADDFFGGGGGF -@M01368:8:000000000-A3GHV:1:1102:10677:23253/1 -CCTTAAATAAGACATCACGA -+ -ABBBBFFFFFFFGGGGGGGC -@M01368:8:000000000-A3GHV:1:1102:13809:1733/1 -ATATTGAACGTAGGTGCGAT -+ -BCCBCFFFFFBFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1102:17584:10050/1 -ATCAAAGACAGATACTGCGA -+ -CCCCCFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1102:18842:24844/1 -CACTTGGGGGTAGCTAAAGT -+ -BBBCBFF@CCBBGGGGGGGG -@M01368:8:000000000-A3GHV:1:1102:21788:11027/1 -GCCCACACGTTCCCCTTAAA -+ -ABCCBCCBBFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1102:5728:8777/1 -ATATTGAACGTAGGTGCGAT -+ -BBBBBFFFFFAFGG5GGGGG -@M01368:8:000000000-A3GHV:1:1102:5983:21093/1 -GTATCCGACATCTGGTTCCT -+ -AAABCFCABBCFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1102:7765:18353/1 -CTCCGGGCCCATAACACTTG -+ -CCDCCCCCCBCFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1103:10405:17879/1 -GTATCCGACATCTGGTTCCT -+ -CBCCCFCCCCCFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1103:13493:8205/1 -GTAGCTAAAGTGAACTGTAT -+ -CCCCCFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1103:14060:21273/1 -GTATCCGACATCTGGTTCCT -+ -AAABAFCCCCCFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1103:14528:12993/1 -GCCCATAACACTTGGGGGTA -+ -BBBBBFFFBDFFGFBCG2EG -@M01368:8:000000000-A3GHV:1:1103:17657:20856/1 -AAAGCGAACTGTATCCGACA -+ -ABB?AABBBBFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1103:23099:3794/1 -AAGACAGATACTGCGACATA -+ -BBBBBFFFFFFFGGEEGGFG -@M01368:8:000000000-A3GHV:1:1103:25465:20010/1 -GACATAGGGTGCTCCGGCTC -+ -ABBBBFFFFBAAGGGFEFGG -@M01368:8:000000000-A3GHV:1:1103:25519:6301/1 -AAATAAGACATCACGATGGA -+ -3A3AAFFFFBFFGGG2CF?4 -@M01368:8:000000000-A3GHV:1:1103:6074:6549/1 -TAACACTTGGGGGTAGCTAA -+ -CCCBCFFFFFCCGGGGGGGG -@M01368:8:000000000-A3GHV:1:1104:11572:8264/1 -GTATCCGACATCTGGTTCCT -+ -BABCBFBACCCFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1104:11928:24197/1 -ACGTAGGTGCGATAAATAAT -+ -CDDCCFCFFFBCGGGGGGGG -@M01368:8:000000000-A3GHV:1:1104:12288:20635/1 -CACTTGGGGGTAGCTAAAGT -+ -ABA?AFBAB2>AFGGGGFFG -@M01368:8:000000000-A3GHV:1:1104:13832:4398/1 -CACGATGGATCACAGGTCTA -+ -AAABABCAFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1104:23670:4161/1 -ATTCAATTGTTATTATTATG -+ -AAAABFFFFFFFGGGGGGGC -@M01368:8:000000000-A3GHV:1:1104:24927:22253/1 -AGCTAAAGTGAACTGTATCC -+ -AAA?AFFF3DFFFGGBGFF3 -@M01368:8:000000000-A3GHV:1:1104:4705:7727/1 -CTACAAGCATTAATTAATTA -+ -BBBBBFFBFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1104:7756:4776/1 -GGCCCATAACACTTGGGGGT -+ -CCCCCCFFFFFFGGGGGGGE -@M01368:8:000000000-A3GHV:1:1105:11310:21761/1 -ATAAATAATAGGATGAGGCA -+ -CCCCBFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1105:11578:14676/1 -ATTGAACGTAGGTGCGATAA -+ -CDDDDFFFBFFCGGGGGGGG -@M01368:8:000000000-A3GHV:1:1105:13575:19255/1 -TTAGTAAGTATGTTCGCCTG -+ -AA1A1DFFFFFBGG3A111F -@M01368:8:000000000-A3GHV:1:1105:15502:11453/1 -GTCCTACAAGCATTAATTAA -+ -A3AAAFFFFFCFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1105:16787:9771/1 -GTTATTATTATGTCCTACAA -+ -AABCCFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1105:17564:15723/1 -TATGCTCGCCTGTAATATTG -+ -EDCCDFFCEEDCGGGGGGGG -@M01368:8:000000000-A3GHV:1:1105:18108:12091/1 -TAATAGGATGAGGCAGGAAT -+ -CCCCCFBFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1105:18331:16683/1 -TCTGGTTCCTACTTCAGGGT -+ -BABA?DFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1105:20577:18421/1 -GGGGGTAGCTAAAGTGAACT -+ -ABB@3>AAFFFFFEAGGGFG -@M01368:8:000000000-A3GHV:1:1105:21099:2895/1 -TAGCTAAAGTGAACTGTATC -+ -CBCBBFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1105:26469:19245/1 -TTATGATTATGTCCTACAAG -+ -BAAAAFFFBFFFGGGGGGFF -@M01368:8:000000000-A3GHV:1:1105:7169:17145/1 -AACCTAAATAGCCCACACGT -+ -CCCCCFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1106:15481:16176/1 -CTTTAGTAAGTATGTTCGCC -+ -ABBBBFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1106:17389:18671/1 -CCATAACACTTGGGGGTAGC -+ -3>>A@FFFFFFFGGGFEECF -@M01368:8:000000000-A3GHV:1:1106:22927:3863/1 -GTATCCGACATCTGGTTCCT -+ -A>ABAFA?D@AFGGGGFGGG -@M01368:8:000000000-A3GHV:1:1106:23625:4236/1 -TAGCTAAAGTGAACTGTATC -+ -@?AAAFFBFBFFFGGFGFGG -@M01368:8:000000000-A3GHV:1:1106:26031:14826/1 -GATCACAGGTCTATCACCCT -+ -ABBAAFFF@FFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1106:27607:15990/1 -GACAGATACTGCGACATAGG -+ -BBBBBFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1106:6455:17124/1 -GTATCCGACATCTGGTTCCT -+ -BBBAAFABDBBFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1107:14451:26748/1 -GTATGTTCGCCTGTAATATT -+ -BABBBFFDABBBGGGGGGGG -@M01368:8:000000000-A3GHV:1:1107:18714:24788/1 -GTATGTTCGCCTGTAATATT -+ -BBBCCFFFDEDEGGGGGGGG -@M01368:8:000000000-A3GHV:1:1107:21355:8333/1 -ATATTGAACGTAGGTGCGAT -+ -ABCBBFFFFFAFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1107:22864:15712/1 -AATAAGACATCACGATGGAT -+ -BBBBBFBBFFFFGGGGCEGG -@M01368:8:000000000-A3GHV:1:1107:24817:14233/1 -CTTCAGGGTCATAAAACCTA -+ -3AABABFFBFFFGCFGGGGG -@M01368:8:000000000-A3GHV:1:1107:24830:14440/1 -AACTGTATCCGACATCTGGT -+ -ABBBA@FFFFBBGGGGGGGG -@M01368:8:000000000-A3GHV:1:1107:26478:23602/1 -GCTAAAGTGAACTGTATCCG -+ -1AAAAF1B13BBBEGGGFGG -@M01368:8:000000000-A3GHV:1:1108:11059:23181/1 -GTTATTATTATGTCCTACAA -+ -CCCCCFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1108:12233:13498/1 -TAGGTGCGATAAATAATAGG -+ -BCCBAFFBCCCCGGGGGGGG -@M01368:8:000000000-A3GHV:1:1108:14328:14751/1 -ATAATAGGATGAGGCAGGAA -+ -BCCCBFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1108:16350:22621/1 -TTAGTAAGTATGTTCGCCTG -+ -AAAACFFFFFFFGGGFGGGG -@M01368:8:000000000-A3GHV:1:1108:21791:23272/1 -CTTTAGTAAGTATGTTCGCC -+ -3ABBBFFFFF@FGGGGGGGC -@M01368:8:000000000-A3GHV:1:1108:23314:26089/1 -ATTGAACGTAGGTGCGATAA -+ -BBBBBFFF?FFAGFBFGGEG -@M01368:8:000000000-A3GHV:1:1108:25084:18127/1 -TTATTATGTCCTACAAGCAT -+ -CCBBCFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1108:7626:10048/1 -ATCAAAGACAGATACTGCGA -+ -CCCCCFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1109:11270:12255/1 -AATCAAAGACAGATACTGCG -+ -CCCCCFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1109:19224:11655/1 -AAATAGCCCACACGTTCCCC -+ -BBB@A4FFFBBBGGGGGGGG -@M01368:8:000000000-A3GHV:1:1109:21978:9490/1 -GGGTCATAAAACCTAAATAG -+ -?A3AA?DFFFFFGGGGGGGF -@M01368:8:000000000-A3GHV:1:1109:22910:4079/1 -TAATAGGATGAGGCAGGAAT -+ -CCCCCFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1109:24217:19077/1 -AAAGTGAACTGTATCCGACA -+ ->AAA3BDFFFFDGBFFEEGG -@M01368:8:000000000-A3GHV:1:1109:25237:11722/1 -CCTGTAATATTGAACGTAGG -+ -AABBBFFFFFBBF5BGGFGA -@M01368:8:000000000-A3GHV:1:1109:27103:7153/1 -GTAATATTGAACGTAGGTGC -+ -33>A?FFFDFBFGCG4EGFG -@M01368:8:000000000-A3GHV:1:1109:8763:7859/1 -TAGTAAGTATGTTCGCCTGT -+ -BABAADFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1110:12808:18367/1 -AATTAACACACTTTAGTAAG -+ -BBBBBFFFFBBFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1110:14751:26540/1 -TAACACTTGGGGGTAGCTAA -+ -AAA?1CFFFFADGEEGFGB1 -@M01368:8:000000000-A3GHV:1:1110:15519:10008/1 -CTGTAATATTGAACGTAGGT -+ -BBBBBFFFFFFFGGGGGGCB -@M01368:8:000000000-A3GHV:1:1110:16559:7507/1 -CATTCAATTGTTATTATTAT -+ -ABBBAFFFFFFFFGGGGGGG -@M01368:8:000000000-A3GHV:1:1110:16700:6843/1 -AAATAATAGGATGAGGCAGG -+ -BBBBBFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1110:18014:7856/1 -ATAAATAATAGGATGAGGCA -+ -AABBBFFFFFFFCGGGCGGG -@M01368:8:000000000-A3GHV:1:1110:23303:11846/1 -ACTTGGGGGTAGCTAAAGTG -+ -3ABB?FAB?DAAGGGFBGGB -@M01368:8:000000000-A3GHV:1:1111:13195:14562/1 -ATCAAAGACAGATACTGCGA -+ -ABAAAFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1111:14287:20817/1 -GCCAACACGTTCCCCTTAAA -+ ->>A3>AAAACFFFGFGFGGG -@M01368:8:000000000-A3GHV:1:1111:23624:14516/1 -GTCCTACAAGCATTAATTAA -+ -3>3AAFFFFFAFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1111:7437:24508/1 -ACACACTTTAGTAAGTATGT -+ -CBCCDCFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1111:8665:25542/1 -TCCTACTTCAGGGTCATAAA -+ -AA3AAFFFFFFFGGGFGFFG -@M01368:8:000000000-A3GHV:1:1111:9655:13916/1 -GGCCCATAACACTTGGGGGT -+ -1>>1AAFFFFFBGGFE1EEE -@M01368:8:000000000-A3GHV:1:1112:10272:7828/1 -GTATCCGACATCTGGTTCCT -+ -AAABABBBBBBFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1112:9832:16531/1 -CTGTAATATTGAACGTAGGT -+ -AABABFFFFFFFF4GGGGEB -@M01368:8:000000000-A3GHV:1:1112:9832:6701/1 -CGCCTGTAATATTGAACGTA -+ -AABBBBFFFFFFGGGFGGGG -@M01368:8:000000000-A3GHV:1:1113:13532:8618/1 -GGAATCAAAGACAGATACTG -+ -AA?AAFFFFFFFGGGGGFBF -@M01368:8:000000000-A3GHV:1:1113:21679:18011/1 -GATCACAGGTCTATCACCCT -+ -AA>1>11F1D1DDFFFFGGG -@M01368:8:000000000-A3GHV:1:1113:25528:14016/1 -CCTACAAGCATTAATTAATT -+ ->AAAAFFFBFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1113:5741:16959/1 -TAGTAAGTATGTTCGCCTGT -+ -ABBBBFFFFFFFGGGFGGGG -@M01368:8:000000000-A3GHV:1:1114:10130:11959/1 -CGTTCCCCTTAAATAAGACA -+ ->A33>FFFFFF@FFFGGGGF -@M01368:8:000000000-A3GHV:1:1114:14540:5315/1 -CTTTAGTAAGTATGTTCGCC -+ -AABCCFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1114:15066:16302/1 -TAAATAAGACATCACGATGG -+ -BBBBAFFBDFBFBGGGGGFG -@M01368:8:000000000-A3GHV:1:1114:16639:15258/1 -CCTAAATAGCCCACACGTTC -+ -11>>ABFFFFFFGGCGC1BB -@M01368:8:000000000-A3GHV:1:1114:2404:13066/1 -TCCTACAAGCATTAATTAAT -+ -CCCCCFFFFCFFGGGGGGGG diff --git a/tools/trim_galore/test-data/paired_hardtrimmed5_pair2_.fastq.gz b/tools/trim_galore/test-data/paired_hardtrimmed5_pair2_.fastq.gz new file mode 100644 index 0000000000..f8295bdb02 Binary files /dev/null and b/tools/trim_galore/test-data/paired_hardtrimmed5_pair2_.fastq.gz differ diff --git a/tools/trim_galore/test-data/paired_hardtrimmed5_pair2_.fastqsanger b/tools/trim_galore/test-data/paired_hardtrimmed5_pair2_.fastqsanger deleted file mode 100644 index b1d8bc7b90..0000000000 --- a/tools/trim_galore/test-data/paired_hardtrimmed5_pair2_.fastqsanger +++ /dev/null @@ -1,396 +0,0 @@ -@M01368:8:000000000-A3GHV:1:1101:6911:8255/1 -ATCTGGTTCCTACTTCAGGG -+ -BCCCCFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1101:14518:9998/1 -GTTATTATTATGTCCTACAA -+ -AAAAAFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1101:18422:19051/1 -GTATCCGACATCTGGTTCCT -+ -CCCCCFDDDDDFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1101:25545:21098/1 -ATTAATTAACACACTTTAGT -+ -33AA?DFD5BDFGGGFEBDG -@M01368:8:000000000-A3GHV:1:1101:5446:12248/1 -AATTAACACACTTTAGTAAG -+ -CCCCDFFFFCCFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1101:5861:6452/1 -ATTATGTCCTACAAGCATTA -+ -ABCCCFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1102:10403:6021/1 -CGCTCCGGGCCCATAACACT -+ ->A@AAAAD2ADDFFGGGGGF -@M01368:8:000000000-A3GHV:1:1102:10677:23253/1 -CCTTAAATAAGACATCACGA -+ -ABBBBFFFFFFFGGGGGGGC -@M01368:8:000000000-A3GHV:1:1102:13809:1733/1 -ATATTGAACGTAGGTGCGAT -+ -BCCBCFFFFFBFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1102:17584:10050/1 -ATCAAAGACAGATACTGCGA -+ -CCCCCFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1102:18842:24844/1 -CACTTGGGGGTAGCTAAAGT -+ -BBBCBFF@CCBBGGGGGGGG -@M01368:8:000000000-A3GHV:1:1102:21788:11027/1 -GCCCACACGTTCCCCTTAAA -+ -ABCCBCCBBFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1102:5728:8777/1 -ATATTGAACGTAGGTGCGAT -+ -BBBBBFFFFFAFGG5GGGGG -@M01368:8:000000000-A3GHV:1:1102:5983:21093/1 -GTATCCGACATCTGGTTCCT -+ -AAABCFCABBCFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1102:7765:18353/1 -CTCCGGGCCCATAACACTTG -+ -CCDCCCCCCBCFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1103:10405:17879/1 -GTATCCGACATCTGGTTCCT -+ -CBCCCFCCCCCFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1103:13493:8205/1 -GTAGCTAAAGTGAACTGTAT -+ -CCCCCFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1103:14060:21273/1 -GTATCCGACATCTGGTTCCT -+ -AAABAFCCCCCFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1103:14528:12993/1 -GCCCATAACACTTGGGGGTA -+ -BBBBBFFFBDFFGFBCG2EG -@M01368:8:000000000-A3GHV:1:1103:17657:20856/1 -AAAGCGAACTGTATCCGACA -+ -ABB?AABBBBFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1103:23099:3794/1 -AAGACAGATACTGCGACATA -+ -BBBBBFFFFFFFGGEEGGFG -@M01368:8:000000000-A3GHV:1:1103:25465:20010/1 -GACATAGGGTGCTCCGGCTC -+ -ABBBBFFFFBAAGGGFEFGG -@M01368:8:000000000-A3GHV:1:1103:25519:6301/1 -AAATAAGACATCACGATGGA -+ -3A3AAFFFFBFFGGG2CF?4 -@M01368:8:000000000-A3GHV:1:1103:6074:6549/1 -TAACACTTGGGGGTAGCTAA -+ -CCCBCFFFFFCCGGGGGGGG -@M01368:8:000000000-A3GHV:1:1104:11572:8264/1 -GTATCCGACATCTGGTTCCT -+ -BABCBFBACCCFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1104:11928:24197/1 -ACGTAGGTGCGATAAATAAT -+ -CDDCCFCFFFBCGGGGGGGG -@M01368:8:000000000-A3GHV:1:1104:12288:20635/1 -CACTTGGGGGTAGCTAAAGT -+ -ABA?AFBAB2>AFGGGGFFG -@M01368:8:000000000-A3GHV:1:1104:13832:4398/1 -CACGATGGATCACAGGTCTA -+ -AAABABCAFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1104:23670:4161/1 -ATTCAATTGTTATTATTATG -+ -AAAABFFFFFFFGGGGGGGC -@M01368:8:000000000-A3GHV:1:1104:24927:22253/1 -AGCTAAAGTGAACTGTATCC -+ -AAA?AFFF3DFFFGGBGFF3 -@M01368:8:000000000-A3GHV:1:1104:4705:7727/1 -CTACAAGCATTAATTAATTA -+ -BBBBBFFBFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1104:7756:4776/1 -GGCCCATAACACTTGGGGGT -+ -CCCCCCFFFFFFGGGGGGGE -@M01368:8:000000000-A3GHV:1:1105:11310:21761/1 -ATAAATAATAGGATGAGGCA -+ -CCCCBFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1105:11578:14676/1 -ATTGAACGTAGGTGCGATAA -+ -CDDDDFFFBFFCGGGGGGGG -@M01368:8:000000000-A3GHV:1:1105:13575:19255/1 -TTAGTAAGTATGTTCGCCTG -+ -AA1A1DFFFFFBGG3A111F -@M01368:8:000000000-A3GHV:1:1105:15502:11453/1 -GTCCTACAAGCATTAATTAA -+ -A3AAAFFFFFCFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1105:16787:9771/1 -GTTATTATTATGTCCTACAA -+ -AABCCFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1105:17564:15723/1 -TATGCTCGCCTGTAATATTG -+ -EDCCDFFCEEDCGGGGGGGG -@M01368:8:000000000-A3GHV:1:1105:18108:12091/1 -TAATAGGATGAGGCAGGAAT -+ -CCCCCFBFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1105:18331:16683/1 -TCTGGTTCCTACTTCAGGGT -+ -BABA?DFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1105:20577:18421/1 -GGGGGTAGCTAAAGTGAACT -+ -ABB@3>AAFFFFFEAGGGFG -@M01368:8:000000000-A3GHV:1:1105:21099:2895/1 -TAGCTAAAGTGAACTGTATC -+ -CBCBBFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1105:26469:19245/1 -TTATGATTATGTCCTACAAG -+ -BAAAAFFFBFFFGGGGGGFF -@M01368:8:000000000-A3GHV:1:1105:7169:17145/1 -AACCTAAATAGCCCACACGT -+ -CCCCCFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1106:15481:16176/1 -CTTTAGTAAGTATGTTCGCC -+ -ABBBBFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1106:17389:18671/1 -CCATAACACTTGGGGGTAGC -+ -3>>A@FFFFFFFGGGFEECF -@M01368:8:000000000-A3GHV:1:1106:22927:3863/1 -GTATCCGACATCTGGTTCCT -+ -A>ABAFA?D@AFGGGGFGGG -@M01368:8:000000000-A3GHV:1:1106:23625:4236/1 -TAGCTAAAGTGAACTGTATC -+ -@?AAAFFBFBFFFGGFGFGG -@M01368:8:000000000-A3GHV:1:1106:26031:14826/1 -GATCACAGGTCTATCACCCT -+ -ABBAAFFF@FFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1106:27607:15990/1 -GACAGATACTGCGACATAGG -+ -BBBBBFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1106:6455:17124/1 -GTATCCGACATCTGGTTCCT -+ -BBBAAFABDBBFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1107:14451:26748/1 -GTATGTTCGCCTGTAATATT -+ -BABBBFFDABBBGGGGGGGG -@M01368:8:000000000-A3GHV:1:1107:18714:24788/1 -GTATGTTCGCCTGTAATATT -+ -BBBCCFFFDEDEGGGGGGGG -@M01368:8:000000000-A3GHV:1:1107:21355:8333/1 -ATATTGAACGTAGGTGCGAT -+ -ABCBBFFFFFAFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1107:22864:15712/1 -AATAAGACATCACGATGGAT -+ -BBBBBFBBFFFFGGGGCEGG -@M01368:8:000000000-A3GHV:1:1107:24817:14233/1 -CTTCAGGGTCATAAAACCTA -+ -3AABABFFBFFFGCFGGGGG -@M01368:8:000000000-A3GHV:1:1107:24830:14440/1 -AACTGTATCCGACATCTGGT -+ -ABBBA@FFFFBBGGGGGGGG -@M01368:8:000000000-A3GHV:1:1107:26478:23602/1 -GCTAAAGTGAACTGTATCCG -+ -1AAAAF1B13BBBEGGGFGG -@M01368:8:000000000-A3GHV:1:1108:11059:23181/1 -GTTATTATTATGTCCTACAA -+ -CCCCCFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1108:12233:13498/1 -TAGGTGCGATAAATAATAGG -+ -BCCBAFFBCCCCGGGGGGGG -@M01368:8:000000000-A3GHV:1:1108:14328:14751/1 -ATAATAGGATGAGGCAGGAA -+ -BCCCBFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1108:16350:22621/1 -TTAGTAAGTATGTTCGCCTG -+ -AAAACFFFFFFFGGGFGGGG -@M01368:8:000000000-A3GHV:1:1108:21791:23272/1 -CTTTAGTAAGTATGTTCGCC -+ -3ABBBFFFFF@FGGGGGGGC -@M01368:8:000000000-A3GHV:1:1108:23314:26089/1 -ATTGAACGTAGGTGCGATAA -+ -BBBBBFFF?FFAGFBFGGEG -@M01368:8:000000000-A3GHV:1:1108:25084:18127/1 -TTATTATGTCCTACAAGCAT -+ -CCBBCFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1108:7626:10048/1 -ATCAAAGACAGATACTGCGA -+ -CCCCCFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1109:11270:12255/1 -AATCAAAGACAGATACTGCG -+ -CCCCCFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1109:19224:11655/1 -AAATAGCCCACACGTTCCCC -+ -BBB@A4FFFBBBGGGGGGGG -@M01368:8:000000000-A3GHV:1:1109:21978:9490/1 -GGGTCATAAAACCTAAATAG -+ -?A3AA?DFFFFFGGGGGGGF -@M01368:8:000000000-A3GHV:1:1109:22910:4079/1 -TAATAGGATGAGGCAGGAAT -+ -CCCCCFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1109:24217:19077/1 -AAAGTGAACTGTATCCGACA -+ ->AAA3BDFFFFDGBFFEEGG -@M01368:8:000000000-A3GHV:1:1109:25237:11722/1 -CCTGTAATATTGAACGTAGG -+ -AABBBFFFFFBBF5BGGFGA -@M01368:8:000000000-A3GHV:1:1109:27103:7153/1 -GTAATATTGAACGTAGGTGC -+ -33>A?FFFDFBFGCG4EGFG -@M01368:8:000000000-A3GHV:1:1109:8763:7859/1 -TAGTAAGTATGTTCGCCTGT -+ -BABAADFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1110:12808:18367/1 -AATTAACACACTTTAGTAAG -+ -BBBBBFFFFBBFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1110:14751:26540/1 -TAACACTTGGGGGTAGCTAA -+ -AAA?1CFFFFADGEEGFGB1 -@M01368:8:000000000-A3GHV:1:1110:15519:10008/1 -CTGTAATATTGAACGTAGGT -+ -BBBBBFFFFFFFGGGGGGCB -@M01368:8:000000000-A3GHV:1:1110:16559:7507/1 -CATTCAATTGTTATTATTAT -+ -ABBBAFFFFFFFFGGGGGGG -@M01368:8:000000000-A3GHV:1:1110:16700:6843/1 -AAATAATAGGATGAGGCAGG -+ -BBBBBFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1110:18014:7856/1 -ATAAATAATAGGATGAGGCA -+ -AABBBFFFFFFFCGGGCGGG -@M01368:8:000000000-A3GHV:1:1110:23303:11846/1 -ACTTGGGGGTAGCTAAAGTG -+ -3ABB?FAB?DAAGGGFBGGB -@M01368:8:000000000-A3GHV:1:1111:13195:14562/1 -ATCAAAGACAGATACTGCGA -+ -ABAAAFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1111:14287:20817/1 -GCCAACACGTTCCCCTTAAA -+ ->>A3>AAAACFFFGFGFGGG -@M01368:8:000000000-A3GHV:1:1111:23624:14516/1 -GTCCTACAAGCATTAATTAA -+ -3>3AAFFFFFAFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1111:7437:24508/1 -ACACACTTTAGTAAGTATGT -+ -CBCCDCFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1111:8665:25542/1 -TCCTACTTCAGGGTCATAAA -+ -AA3AAFFFFFFFGGGFGFFG -@M01368:8:000000000-A3GHV:1:1111:9655:13916/1 -GGCCCATAACACTTGGGGGT -+ -1>>1AAFFFFFBGGFE1EEE -@M01368:8:000000000-A3GHV:1:1112:10272:7828/1 -GTATCCGACATCTGGTTCCT -+ -AAABABBBBBBFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1112:9832:16531/1 -CTGTAATATTGAACGTAGGT -+ -AABABFFFFFFFF4GGGGEB -@M01368:8:000000000-A3GHV:1:1112:9832:6701/1 -CGCCTGTAATATTGAACGTA -+ -AABBBBFFFFFFGGGFGGGG -@M01368:8:000000000-A3GHV:1:1113:13532:8618/1 -GGAATCAAAGACAGATACTG -+ -AA?AAFFFFFFFGGGGGFBF -@M01368:8:000000000-A3GHV:1:1113:21679:18011/1 -GATCACAGGTCTATCACCCT -+ -AA>1>11F1D1DDFFFFGGG -@M01368:8:000000000-A3GHV:1:1113:25528:14016/1 -CCTACAAGCATTAATTAATT -+ ->AAAAFFFBFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1113:5741:16959/1 -TAGTAAGTATGTTCGCCTGT -+ -ABBBBFFFFFFFGGGFGGGG -@M01368:8:000000000-A3GHV:1:1114:10130:11959/1 -CGTTCCCCTTAAATAAGACA -+ ->A33>FFFFFF@FFFGGGGF -@M01368:8:000000000-A3GHV:1:1114:14540:5315/1 -CTTTAGTAAGTATGTTCGCC -+ -AABCCFFFFFFFGGGGGGGG -@M01368:8:000000000-A3GHV:1:1114:15066:16302/1 -TAAATAAGACATCACGATGG -+ -BBBBAFFBDFBFBGGGGGFG -@M01368:8:000000000-A3GHV:1:1114:16639:15258/1 -CCTAAATAGCCCACACGTTC -+ -11>>ABFFFFFFGGCGC1BB -@M01368:8:000000000-A3GHV:1:1114:2404:13066/1 -TCCTACAAGCATTAATTAAT -+ -CCCCCFFFFCFFGGGGGGGG diff --git a/tools/trim_galore/test-data/sanger_full_range_report_results1.txt b/tools/trim_galore/test-data/sanger_full_range_report_results1.txt index 29ee8cf9a4..9ff7587285 100644 --- a/tools/trim_galore/test-data/sanger_full_range_report_results1.txt +++ b/tools/trim_galore/test-data/sanger_full_range_report_results1.txt @@ -1,57 +1,60 @@ - SUMMARISING RUN PARAMETERS -========================== +========================= Input filename: input_1.fastq Trimming mode: single-end -Trim Galore version: 0.6.10 -Cutadapt version: 5.0 -Python version: 3.12.10 -Number of cores used for trimming: 4 +Trim Galore version: 2.2.0 (Oxidized Edition) Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 -Unable to auto-detect most prominent adapter from the first specified file (count smallRNA: 0, count Nextera: 0, count Illumina: 0) -Defaulting to Illumina universal adapter ( AGATCGGAAGAGC ). Specify -a SEQUENCE to avoid this behavior). -Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; default (inconclusive auto-detection)) -Maximum trimming error rate: 0.1 (default) +Adapter sequence: 'AGATCGGAAGAGC' (user-specified or auto-detected) +Maximum trimming error rate: 0.1 Minimum required adapter overlap (stringency): 1 bp -Minimum required sequence length before a sequence gets removed: 20 bp +Minimum required sequence length single-end: 20 bp -This is cutadapt 5.0 with Python 3.12.10 -Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC input_1.fastq -Processing single-end reads on 4 cores ... +Trim Galore 2.2.0 (Oxidized Edition) — adapter trimming built in +This is cutadapt 4.0 (compatible; for MultiQC backwards compatibility) +Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC input_1.fastq +Processing reads on 1 core in single-end mode ... === Summary === -Total reads processed: 2 -Reads with adapters: 1 (50.0%) -Reads written (passing filters): 2 (100.0%) +Total reads processed: 2 +Reads with adapters: 1 (50.0%) +Reads written (passing filters): 2 (100.0%) -Total basepairs processed: 188 bp -Quality-trimmed: 20 bp (10.6%) -Total written (filtered): 167 bp (88.8%) +Total basepairs processed: 188 bp +Quality-trimmed: 20 bp (10.6%) +Total written (filtered): 167 bp (88.8%) === Adapter 1 === -Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 1 times +Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 1 times. -Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 -Bases preceding removed adapters: - A: 0.0% - C: 100.0% - G: 0.0% - T: 0.0% - none/other: 0.0% - Overview of removed sequences length count expect max.err error counts 1 1 0.5 0 1 + RUN STATISTICS FOR INPUT FILE: input_1.fastq ============================================= 2 sequences processed in total Sequences removed because they became shorter than the length cutoff of 20 bp: 0 (0.0%) +Sequences removed because they were longer than the upper length cutoff: 0 (0.0%) +Sequences removed because of too many N bases: 0 (0.0%) + + +Overview of removed sequences +length count expect max.err error counts +1 1 0.5 0 1 + + +RUN STATISTICS FOR INPUT FILE: sanger_full_range_original_sanger.fastqsanger +============================================= +2 sequences processed in total +Sequences removed because they became shorter than the length cutoff of 20 bp: 0 (0.0%) +Sequences removed because they were longer than the upper length cutoff: 0 (0.0%) +Sequences removed because of too many N bases: 0 (0.0%) diff --git a/tools/trim_galore/test-data/sanger_full_range_report_results1gz.txt b/tools/trim_galore/test-data/sanger_full_range_report_results1gz.txt index 52a80d3192..154c0488d4 100644 --- a/tools/trim_galore/test-data/sanger_full_range_report_results1gz.txt +++ b/tools/trim_galore/test-data/sanger_full_range_report_results1gz.txt @@ -1,58 +1,48 @@ - SUMMARISING RUN PARAMETERS -========================== +========================= Input filename: input_1.fastq.gz Trimming mode: single-end -Trim Galore version: 0.6.10 -Cutadapt version: 5.0 -Python version: 3.12.10 -Number of cores used for trimming: 4 +Trim Galore version: 2.2.0 (Oxidized Edition) Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 -Unable to auto-detect most prominent adapter from the first specified file (count Illumina: 0, count smallRNA: 0, count Nextera: 0) -Defaulting to Illumina universal adapter ( AGATCGGAAGAGC ). Specify -a SEQUENCE to avoid this behavior). -Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; default (inconclusive auto-detection)) -Maximum trimming error rate: 0.1 (default) +Adapter sequence: 'AGATCGGAAGAGC' (user-specified or auto-detected) +Maximum trimming error rate: 0.1 Minimum required adapter overlap (stringency): 1 bp -Minimum required sequence length before a sequence gets removed: 20 bp +Minimum required sequence length single-end: 20 bp Output file will be GZIP compressed -This is cutadapt 5.0 with Python 3.12.10 -Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC input_1.fastq.gz -Processing single-end reads on 4 cores ... +Trim Galore 2.2.0 (Oxidized Edition) — adapter trimming built in +This is cutadapt 4.0 (compatible; for MultiQC backwards compatibility) +Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC input_1.fastq.gz +Processing reads on 1 core in single-end mode ... === Summary === -Total reads processed: 2 -Reads with adapters: 1 (50.0%) -Reads written (passing filters): 2 (100.0%) +Total reads processed: 2 +Reads with adapters: 1 (50.0%) +Reads written (passing filters): 2 (100.0%) -Total basepairs processed: 188 bp -Quality-trimmed: 20 bp (10.6%) -Total written (filtered): 167 bp (88.8%) +Total basepairs processed: 188 bp +Quality-trimmed: 20 bp (10.6%) +Total written (filtered): 167 bp (88.8%) === Adapter 1 === -Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 1 times +Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 1 times. -Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-13 bp: 1 -Bases preceding removed adapters: - A: 0.0% - C: 100.0% - G: 0.0% - T: 0.0% - none/other: 0.0% - Overview of removed sequences length count expect max.err error counts 1 1 0.5 0 1 + RUN STATISTICS FOR INPUT FILE: input_1.fastq.gz ============================================= 2 sequences processed in total Sequences removed because they became shorter than the length cutoff of 20 bp: 0 (0.0%) +Sequences removed because they were longer than the upper length cutoff: 0 (0.0%) +Sequences removed because of too many N bases: 0 (0.0%) diff --git a/tools/trim_galore/test-data/sanger_full_range_results1.fastq.gz b/tools/trim_galore/test-data/sanger_full_range_results1.fastq.gz index 2a7f9fdd63..08f7272355 100644 Binary files a/tools/trim_galore/test-data/sanger_full_range_results1.fastq.gz and b/tools/trim_galore/test-data/sanger_full_range_results1.fastq.gz differ diff --git a/tools/trim_galore/test-data/sanger_full_range_results1.fastqsanger b/tools/trim_galore/test-data/sanger_full_range_results1.fastqsanger deleted file mode 100644 index 1b5d317c77..0000000000 --- a/tools/trim_galore/test-data/sanger_full_range_results1.fastqsanger +++ /dev/null @@ -1,8 +0,0 @@ -@FAKE0001 Original version has PHRED scores from 0 to 93 inclusive (in that order) -ACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTAC -+ -!"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~ -@FAKE0002 Original version has PHRED scores from 93 to 0 inclusive (in that order) -CATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGC -+ -~}|{zyxwvutsrqponmlkjihgfedcba`_^]\[ZYXWVUTSRQPONMLKJIHGFEDCBA@?>=<;:9876 diff --git a/tools/trim_galore/test-data/sanger_full_range_results2.fastq.gz b/tools/trim_galore/test-data/sanger_full_range_results2.fastq.gz index 1a99365ec3..08f7272355 100644 Binary files a/tools/trim_galore/test-data/sanger_full_range_results2.fastq.gz and b/tools/trim_galore/test-data/sanger_full_range_results2.fastq.gz differ diff --git a/tools/trim_galore/test-data/sanger_full_range_results2.fastqsanger b/tools/trim_galore/test-data/sanger_full_range_results2.fastqsanger deleted file mode 100644 index 1b5d317c77..0000000000 --- a/tools/trim_galore/test-data/sanger_full_range_results2.fastqsanger +++ /dev/null @@ -1,8 +0,0 @@ -@FAKE0001 Original version has PHRED scores from 0 to 93 inclusive (in that order) -ACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTAC -+ -!"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~ -@FAKE0002 Original version has PHRED scores from 93 to 0 inclusive (in that order) -CATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGC -+ -~}|{zyxwvutsrqponmlkjihgfedcba`_^]\[ZYXWVUTSRQPONMLKJIHGFEDCBA@?>=<;:9876 diff --git a/tools/trim_galore/test-data/sanger_full_range_results3.fastq.gz b/tools/trim_galore/test-data/sanger_full_range_results3.fastq.gz index 3126775b6a..08f7272355 100644 Binary files a/tools/trim_galore/test-data/sanger_full_range_results3.fastq.gz and b/tools/trim_galore/test-data/sanger_full_range_results3.fastq.gz differ diff --git a/tools/trim_galore/test-data/sanger_full_range_results3.fastqsanger b/tools/trim_galore/test-data/sanger_full_range_results3.fastqsanger deleted file mode 100644 index 1b5d317c77..0000000000 --- a/tools/trim_galore/test-data/sanger_full_range_results3.fastqsanger +++ /dev/null @@ -1,8 +0,0 @@ -@FAKE0001 Original version has PHRED scores from 0 to 93 inclusive (in that order) -ACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTAC -+ -!"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~ -@FAKE0002 Original version has PHRED scores from 93 to 0 inclusive (in that order) -CATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGC -+ -~}|{zyxwvutsrqponmlkjihgfedcba`_^]\[ZYXWVUTSRQPONMLKJIHGFEDCBA@?>=<;:9876 diff --git a/tools/trim_galore/test-data/trimmed_polyA_reads_pair1.fastq.gz b/tools/trim_galore/test-data/trimmed_polyA_reads_pair1.fastq.gz new file mode 100644 index 0000000000..17020342c8 Binary files /dev/null and b/tools/trim_galore/test-data/trimmed_polyA_reads_pair1.fastq.gz differ diff --git a/tools/trim_galore/test-data/trimmed_polyA_reads_pair1.fastqsanger b/tools/trim_galore/test-data/trimmed_polyA_reads_pair1.fastqsanger deleted file mode 100644 index 490febc003..0000000000 --- a/tools/trim_galore/test-data/trimmed_polyA_reads_pair1.fastqsanger +++ /dev/null @@ -1,396 +0,0 @@ -@M01368:8:000000000-A3GHV:1:1101:6911:8255/1 -ATCTGGTTCCTACTTCAGGGCCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATT -+ -BCCCCFFFFFFFGGGGGGGGGGGHHHHGHGHHHHHHHHHGGGGGGHHHHGHHHHHHHHHHGHHHHHHGGHGGHHHGHHHHFHHGHHHHHHHHHGHEHEFFGHHEGGCEFGGFHHHBGHHGHHHHGHFHHHGHGHGHGGCDFDDACGGGGGGGAAFFFFFFFFFBAFFFFFB;FFFFFFADDFFFFFFFFFFEFFFFFFFFFFBFFFFFFFFFFFFFFEFFFFFFFFBFEFFFFEFE;DFFFDFBFF/9BFB -@M01368:8:000000000-A3GHV:1:1101:14518:9998/1 -GTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGAT -+ -AAAAAFFFFFFFGGGGGGGGGGHGGHHHHGHHHHHHHGCGHHHHHHHHHHHHHHHGGGGGHHHHHHHHHGHHGFHFE5BGEEHFGGGHHHHHHHHFBHHGGGGFHGHHFGHHHHGHHHHHHGEGGGGFHFHGEGHHGGCDGDGHGGGDGGHGGCGGGHGHHH/ACDG?.1FGCDCCGCA.CC@CDCHFHGFFGGGEBFGAB//CEFBFGG.:;D;;A0AFFFFFB..:@ABFF//;BFFFFFBF/9D:A// -@M01368:8:000000000-A3GHV:1:1101:18422:19051/1 -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG -+ -CCCCCFDDDDDFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHFHHHHGGGGHHHHHHHHHGHHHHHHHHHHHHGGHGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHGCGGGHHHHHHHHHHHHHHHHHHHHHHGFDHGFHCFGGGGFGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF;FFFFFFFFFFFFFFFFFFFFFFFFFFFFEFBFFFFFFFFFF:FFF. -@M01368:8:000000000-A3GHV:1:1101:25545:21098/1 -ATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATAAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGGCTTATTTAAGGGGAACGGGTGGGG -+ -33AA?DFD5BDFGGGFEBDGEGHEGHGEGHCEGGHHCHGHHFFHHGFGAGE53FF2FAFFGDE5FFFE5GFBFGAEE1GHHHGHHHEHE3FGHF@GEGEGGHHGG3FAGFFDE?EEE3GFEGFGFGGCG?GHHHFHGGGC@DHFFHD/AA@AAAAD2ADDFFGGGGGFGGHA?EAEFBEAGHFABFGG5FDF5DB1EEGAFDFB53FF5FH@G5FFEHGHEFHFFHBE333GF43GCGGGGE@0?BFGGB0B?FHGFDGGHHHBFFDEGGHGFFFDFE@<1>@FFFGHHHHFHEFGDABFFGG/@DCE---;--..;.../9 -@M01368:8:000000000-A3GHV:1:1103:25519:6301/1 -AAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGCATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATTTCGT -+ -3A3AAFFFFBFFGGG2CF?4FGHGHHCHGGHHBHGFFAGGHHFFHHCGHFEGCECEGHFFFHGHHGFGHFGDAFDGFFEEFEGGBECC/;>=EF?AEFFFF;9;=DAFFFFBBFFFBFFF//;BFB.:FBFFBB/BFFFF/BFFE?EFAFEF9 -@1:A:M01368:8:000000000-A3GHV:1:1104:11928:24197/1_PolyA:1 -ACGTAGGTGCGATAAATAATGGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGAT -+ -CDDCCFCFFFBCGGGGGGGGGGHHHGHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHGGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHGHHHHGHHGHHHHGGHGEEGGGGFHHFHHHGHGEHHHHHHHHHHHHHHHHHGGGGGGFGGGGGGGGFFGGGFGFGAEFEFGFEAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF@D -@M01368:8:000000000-A3GHV:1:1104:12288:20635/1 -CACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGCCGCAGCATCTGTCTTTGATTCCTGCCT -+ -ABA?AFBAB2>AFGGGGFFGD5GGHGFFHGHEGGGGHHHHFFHH5FHGHHHFFHEFHHHHHHGGHHHHHGFHGGHGGGGFHGGGFBHHHFGEGHHHHGHGGGGECEHHFHHGHHFFHFGGGHHHEGHDGHHGHGHFHHHECGCBCHHGFHHFHD1GFHEFHEF?GDCFFEFE0A@;@>BD/9BFBBBBFDFFFF99D=?>DDBFF-AFFEBB9BBEFEFAFFF//-@---;;FBBFBFB///;99999BA9 -@M01368:8:000000000-A3GHV:1:1104:13832:4398/1 -CACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGACAAAAAAAAACACACAACAACCAACACCCAGTAGACTAGTGCGTC -+ -AAABABCAFFFFGGGGGGGGGGHFHHHHHHHHHGFFHHGGHGGGGGHHHHHHHHHHFHHHHGHHHHHGGHGHGGHGGGGGGGHGFFGGGGDGGHHHHGGGGFGGGGGCFGGEGCDGGGGGGGGGGGGEGF?;BFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF/FFFFFFC.;BFEAFFFFF;@FFBFFFFEF?/FFFFE----:...:...9....9.9...:.9//9////;/9-.- -@M01368:8:000000000-A3GHV:1:1104:23670:4161/1 -ATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCC -+ -AAAABFFFFFFFGGGGGGGCGBBGGHHHHHHHHHGHHHHHHHHHHFGGHHHHHHGGFHHHHHHHGGGGGFHHHGHHHHHGHHHHGHHGGGHGGGGHHHHHGHHHHHHEGGGFHHHGFFDGEGHGHFHGHHGEGGGHHBGHGGHGHGGGGG/@FCDFDGFHGGGDFFHFGFFGGGGGHHHHGGGGGGGEGCDG?GGBFFFEE00BFFGABBBAFGGGEGEA.9FFF/./;BFBF./AAF/BFFBBF/F/BFB -@M01368:8:000000000-A3GHV:1:1104:24927:22253/1 -AGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTAT -+ -AAA?AFFF3DFFFGGBGFF3EAEEEHFHHFHHHGHHHHDGHHGAEFGHHHGFCHHGHHHBGHHHAEGGHGGFHFBFHHHHHHHHHHFHH1EEHEEHH1FFDEEFCGHBGHHFHEHHHHHH1FCGHHHHEEFGEEGFFCHHH11>GHHF/FG2DGGCGAGGHGGGG@@@BEFFF?@@@FFEFFFFFF?@@FB@BB@FFFE=;==---A9EFBBB/;E;A9B/B/FFFEF/B///;/;9/A/;/9BFFFBFBB -@M01368:8:000000000-A3GHV:1:1104:4705:7727/1 -CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG -+ -BBBBBFFBFFFFGGGGGGGGGGHHHGHHHGHHHHH6GHHHHFFGGGHHHHHHHHHHHHHHHGGFGGGEGGGHHHHHHHHHHGHE3FGGGGHHHHHFHHHHHHHHHHHHGGGGGHHFHFCGFHHGGGGGGGHHGGGGHHGGGGGGHHHHGGGADCHHHHGDGGGGGGGGC@DGFHEHHHHHHGHHHHGFGDEGFGFFGGGGCFGBEGFGGGFG;C.BFFFFFFFBFBFFFF/F?.AFFF/BFFEFFFFFEFF -@1:A:M01368:8:000000000-A3GHV:1:1104:7756:4776/1_PolyA:1 -GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTG -+ -CCCCCCFFFFFFGGGGGGGEEFHHHHGHGHHHHHHGGHHHGGGGGGHHGHHHHHGHHGHHHGGFHHHHHHHHGHHHFHHGHHGFGGGGHHHHHHHHGHGHFFHFHHHHHHGGHGFGFHHHGHG?FGHHHHHHHHHHHHHHGHGHHGHGGCDGFFHHHHGHHHHHGHHHGFHGHFHGHGHGH/@CABFFFFFFFACAFF?BBFFBFD-@-.D>FFAF.F.:9---;AADFFFFFBEFFF:9FFFFBBFFFF -@M01368:8:000000000-A3GHV:1:1105:11310:21761/1 -ATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTT -+ -CCCCBFFFFFFFGGGGGGGGGGGHHHHHHHHHHHHGHHHHHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHGHHHGHHHGHGHHGHHHHGGHGGG=FFGEHHHHHGHFGGHHGHHHHHFHHHHHHHGGHHHHHHHGHEHHGHHHHHGGGGF9AGE?DEGFFGGGGGGGGGGGGGGFFFF/9FFBFFFFFFFEFFFBFFFFFFFFFFFFEFFF0 -@M01368:8:000000000-A3GHV:1:1105:11578:14676/1 -ATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTC -+ -CDDDDFFFBFFCGGGGGGGGGGHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHFGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHHHHHHHHHGHHHHGGGGGGHGGHGHHHHHHH;CHHHHHHHHHHHHHHHGHGGGGFGGGFGGGGGGGGGGGGFGGGEGGFFFFFFFFFFFFFFFFFFFFFFFFF/FFFFFFFFFFFFFFFF0 -@M01368:8:000000000-A3GHV:1:1105:13575:19255/1 -TTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGTTCCCGTGAGTGGTTAATAGGGTGATAGGCCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG -+ -AA1A1DFFFFFBGG3A111FFAEGDDBGFDHF00F01BBA00BFGEHFBFFA1FABB0FAE?CFHF1BGFHFB0011F121E//>>1F10>B?EFHGCC/?/BFG/EEGFFEE//BCFDE0?E@@B/?11AAFFFFFEAGGGFGGAA6FHDGGCGFHFFHFHFHHHHHGDDDFFFHGFFHGBEGEGHHHFFFHGEEAEGHHHHHFHHHFHGHFEDGGEGBEAGEFHHFFHHHHHHGGHHHGHHGBGHHFHHFGHHHHG?CG?AFHH1FHHHGHFHHHF?DGHFFHCGAGEFHGCBBBB?BFFF?B-B.9/BFFFFFB=-AD?..@FFFF@@-@@?AFD.FFFBBEEBD9/:FFFFFBB/:FF9BF/BF.BBFF/: -@M01368:8:000000000-A3GHV:1:1105:21099:2895/1 -TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTT -+ -CBCBBFFFFFFFGGGGGGGGGGGGGFHHHHFHGHHHHHHFHHHHFGFHHHHHHGHHHHHHGHHEHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHHHHHHHFHHHHGHGHHHGGHHHHHGGHFFGHGCGGGHGHHGHHHFGHHFHH/FGGGHFGFEGGCFCCD;B@DFFFFFFFFFFFFFFFFFFFFFFFFF;F.BEFADFFFF.AEFFFFFFE;D.;:B00099:09BFBFFBFF.0:000BFFBF0 -@M01368:8:000000000-A3GHV:1:1105:26469:19245/1 -TTATGATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCAAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATG -+ -BAAAAFFFBFFFGGGGGGFFGGHHHHHHHHHHHHGGHHGHHHGGHHHHHHHHHHHDEGGHGHHHHHGEDHFHGFHGGHHGGHGGEHHHGHHGHHGHHHGGEGGGHGHGHHHHHHGHHHHGHGGGGGHFHGGGGHHGGGGCDGHHHGD>A@FFFFFFFGGGFEECFGGFFB5GBHH5DFH5GBEEAEFHFHHF3F3GHFHHHEF3GAEHBGFHFFEFFHHHHFFB31FGGEGFHGEHHHHHHHHHHHHHHHHHEEEEGHHHFFF3GF3FGF44BBGEHHHHGG2B/BGGHE;B=.9.;.BBFFF..;A;/B/;B//;;//BBF/ -@2:A:M01368:8:000000000-A3GHV:1:1106:22927:3863/1_PolyA:2 -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGACTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT -+ -A>ABAFA?D@AFGGGGFGGGFGFHHHFFH4BCBGHHHHGFFF3G5BF3322EEAFHHHHHHAB5GHFHEHFHHH3BEEHFGF5@13FDF3GDGFHHFGHHHGHHHGFGGHHFCG//?/EGHHHG3F4BFFGHHHEDGFHHB/B/?FBF<@-9?BDEFFF?B--?DFFFB9FA>B;A.BFDB?AB.--@;-.FFAEFFFF9AE;DDFFFFB/9BFFFFFFFB:BF/BB/BFF//FFF-=--.99=--.9/ -@1:A:M01368:8:000000000-A3GHV:1:1106:23625:4236/1_PolyA:1 -TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATT -+ -@?AAAFFBFBFFFGGFGFGGGEFCGGHHHHFGHGHFHGHHHHGGFGGFGHHGHGHHHHGHGFFHHGGGGGHHHHHHHHHBFHHEFHHHHHHGF?FAGHGFHHHGDGFHHGGGHGHFHHHGHHHGHHFHHGFFGFHHHGHHHHFFHHHHH/FGHHHHGHGHGDFFFE?FFF/--;;9/:BFFFBA///9----;B.//////;...---9-- -@M01368:8:000000000-A3GHV:1:1106:6455:17124/1 -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG -+ -BBBAAFABDBBFGGGGGGGGGGHHHGHHGHHHHHHHHHGHHGHHGHHHGHGGGEGHHHHHHHHHHHHHGHHHGHHHGGHGGHHHHHHHHHHHHHHHHHHGHHHHHHHHGHHHHHGGCFEHHHGFHHHHGHHFFH?GGHHHHGHGHGHHCFGGGGGGGFFFFFFFFFFFFFFFFFFFFADFA?.FEFAFFFEFFFFFFFFFFFFDABFBBFFFFFFFFEFFBFFFFFFFFFFF/FFBFFFFFFADE>BFFF9 -@1:A:M01368:8:000000000-A3GHV:1:1107:14451:26748/1_PolyA:1 -GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTG -+ -BABBBFFDABBBGGGGGGGGGGHGHHHHGAGHGGGGGHHHHHHHHGHHHHHGGGGGGFFHHHHHHHHHFHHHHHGGGGCHFHH3FEHHGCGGGDGHHHGFGGHHGGGGHHHHHHG/DGGGHHHHGGGGGCCHGG?CGG1GHHGHHHHBGHHHFHHGHHHHGGADAD00ECEGHEFHHFFAEFFFFFGGFBFBFFFGFFG.;B/99;BCFGGGGEFFFFFFEDA./:ADEB?FBFFFF;FFEBFBFFB./9 -@1:A:M01368:8:000000000-A3GHV:1:1107:18714:24788/1_PolyA:1 -GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTG -+ -BBBCCFFFDEDEGGGGGGGGGGHHHHHHGGHHGGHGGHHHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHCFGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHGHHHHHHHHHHHGHHHHGGEGEGCCEGGHHHHHHG:CAFFGGGGGGBFEFGGGGHGAFFGGFGGGGGGGEFFFFFFFFFEF/EDDFFFFFFFFFBFFFFFBBFFBBF -@M01368:8:000000000-A3GHV:1:1107:21355:8333/1 -ATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATG -+ -ABCBBFFFFFAFGGGGGGGGGGHHHHHHHHFHHHHHGGGGGHHHHHHFHHHGHHHHHHHGGGGGHHHHFFGHGHGGGGGGGHGGEGGGHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHGHHHFHHHHHHHHGHFFHHHHGGHGEGHHGHGFHHFHGHCEFHEGHGHHHGHHHGGGGGGCFFFFBFGGFGGGGFBG?FDGGGEGFFFFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFF.AA/9 -@M01368:8:000000000-A3GHV:1:1107:22864:15712/1 -AATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG -+ -BBBBBFBBFFFFGGGGCEGGGGHGHHDGFHHHHHHHHHHHHHCHHGHFGBGDFGE?GHHGHFBHHFHHHHHHHHHGHFGFGHGHHGGC@DCFGHHGGGGGGGHHHHHHGGGGGFGGGGFFGGGGFADGFEFFFFFFFFFFF9;FFFF/BFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFEAECDFFFFFFFFFBFFFFFFFFFFFFFFEFBFBFFF/FFFDF-;-9;E@>-9DFFF/FFBF;-.:;.9A// -@M01368:8:000000000-A3GHV:1:1107:24817:14233/1 -CTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACAT -+ -3AABABFFBFFFGCFGGGGGGGHHHFGCFGGGFFGHHCGHHHGHGHGHBFFHHHEDGGFGFGHHFFFHGGEGHFHHGHFHGFFHHHFGHHHFHACECEHHHBGFHGFHHHFHHF4GFHFHFHEHGGE/EG:A-CFGGFGADGGGGFGGGGDAAAADFA.-AFFECFDAAFFFFEFFFFBFF;D;BFFFB/;BFFFFFFFFFFFFFFBFFBF/;//;BFFFF?FFFFFFEDAFFFFFBF//:BFBACF--9/ -@M01368:8:000000000-A3GHV:1:1107:24830:14440/1 -AACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACC -+ -ABBBA@FFFFBBGGGGGGGGGGHGHHGHHGHHHGGHHHHHGHGHHGDHHHHHHAGGGGFHHGHHHHHHGHHHHHHHHHHHEGAFGFHFHHHHGEGHHHHHHHHHHHHHHHHHGGHHHGEFEGGHHGHHHHHGGHGFHH/?GHHHHGFGHGFHCGGBBDAFFFEFFFFFFFFFFFFFF=CDDADDFFFFEF=BFFFEFFFBFFFFEEBF./:FFFFFFFFFFFFFFFFFFFEFFFFFFFFFFF/BFBEFFC- -@M01368:8:000000000-A3GHV:1:1107:26478:23602/1 -GCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATCTGTCTCTTATACACCTCTC -+ -1AAAAF1B13BBBEGGGFGGGGGGHHDB1BGFHHHFGFGGG0F00BGBGFGGF1GBGB1BGCGAEE/FGFHGHFHHHHHBFF1B0FGGF//F//FGFGFFF/1@B1DFHHHGGEFEDHFDFAFF0B//1FDD1GFFHHDF?1BD>GF/GCAC0C@?@-;99BBB->@--;-B//99B--9-BF=A--A-:A----9AA9AFBFBBB?E@BBFFFFFFFBFFFFFBFFFBBFFF//B/////-;9 -@M01368:8:000000000-A3GHV:1:1108:11059:23181/1 -GTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGAT -+ -CCCCCFFFFFFFGGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHGHHGGGGHIIIHHHHHHHGHHHHGHHHGGHGGHHHHHHHHHHHHHHGGGGGHHGHHHHHHHHHHHHHHHGGGGGHHHHHFGHHHGGGGGGHHHGGGGGHGGGGHHHHHHGGGGGGHGGHEFGGGGGHGGG-ECHHHGGGGGGGGGFGFFGGGGFGGGCF?EFGG0;BFFFFB?9AAFFFFFFBF;BBFFBFFFFE?F/ -@M01368:8:000000000-A3GHV:1:1108:12233:13498/1 -TAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCCTGTCTCTTATACAC -+ -BCCBAFFBCCCCGGGGGGGGGGHHHHGGGGGHHHHHHGHGHBGFHHHHHGGGGGHHGGBFGHHGGGGGGGHHHGGGGHHGGGFHHHHHHGGGGGGHGHHGGGGGGGGGGGGGGHHHHFHHHGHGHHHHHHGHGHHGGFGGGDFFGGHHHGHGH/FHHHFHGHGHHGHHHGHHBAAAA3BDFFFFDGBFFEEGGGGHHGHFHHCFHGHGHHHGF3BFGFHGFEFHFHGH3EGFC1EEGBFGBHFGGFFHHHGHFHGHHGHGGHCEHEBCFF3ED3@GH4FDEG12FFHFFHG/G3FGA/@EFEFCG33FFGBBGHFHH/?BFBGFGFAGC2C/@-;@-;BFFEFADF?DFFFFFF?;AFFA;AD;..FEFFA--;9;D.9BFFBE?;.;///://///99//BB/9;F./B//BBFB9////99/ -@M01368:8:000000000-A3GHV:1:1109:25237:11722/1 -CCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATATCGTATGCCGT -+ -AABBBFFFFFBBF5BGGFGABGHGGAEFGFHHHHFHCHFHHBFGGGEHBGGHFFFFGFAEF5FHH?EGGF5AEG3?EBFHD?C/EFHFFEGEE?CFGGGHHFHB3EEFG?EEGHCFGGGGGGGECA?FFFDFBFGCG4EGFG??GACFGGHFHCFHCHBECDEFCEGHHHHHFFGGF3AFFHHFGEGCGHHGEHGFGHHEGG?EFHHH?EGGHHGGGGHHHHHHEGGGGGHFHHGFDDGGACFCC@?/FDDGHHHGHEFFHHH0FDHHFFEDCD.CFHHGDFGGGGGEFGC?EGHHHB0CGHHGCGHHHHBEHGHH.::ACA;FECFGGGGGFFFEFGGGGGGGFFBBBFGBFFFEFFFFBFFFFFFBBFBFFB9..AF?FFFFFFF9 -@M01368:8:000000000-A3GHV:1:1110:14751:26540/1 -TAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACTGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGTTGTGCACGCGATAGCATTGCGCGACGCTGGCGCCGGCGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTG -+ -AAA?1CFFFFADGEEGFGB1112DGDGHGFGHHCG/ECEBGGHDFGHHFHGHEB1BCGFFE2EGHHGHFFFHHBFFHEFCGGHHFHHHHH111BFFHGHEHBEHGGECEFHGFHFGGDBBGHHBHHHH0GH22FGHGFFFH1BFCG0F@FGG11@GDHFG1D/1FGFHH/FCG.>0><-<@CCFFG.CC-@C?C9FFF0C---;------;9----9-9@-AAFF9B9---9-/;B9BF/9BB//B/;/// -@M01368:8:000000000-A3GHV:1:1110:15519:10008/1 -CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACC -+ -BBBBBFFFFFFFGGGGGGCBGGE?GGGHHHHHGHHBEGGHHGGGGGHHHHHHHHHHHGGHHHHGFGGGGHGHGCFGHHHGGGCGGHGHGGGGGHFGGGHHHHHHGCEFGGHHHHGGGGGGGHGG/CEGHHHHGFHHHGFHHGFFHGHGHFGGEGFDDGGBCGHHGFHH/CHHHHGHGHF0CFGGGGGGGFFFFFBFFFFFFGFFGGF?DGEDEEFEFFFF?FFFFFFFFFFFFFFFBFFF.BFFFBFBBA; -@M01368:8:000000000-A3GHV:1:1110:16559:7507/1 -CATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAACTGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATC -+ -ABBBAFFFFFFFFGGGGGGGFGGGGAGHHGCHHGHHHHHHHHHHHGHHGHHHHHHHHHHHHHHHHFGGGHHHHHHHHBDHHHHHGGHFEEGHGGHHHHHHHGHHHEHFGGGGGGHHHF33FGHHG3GDDGH//EEGHHFFBF?GHHFCC@DGFCGGB?DGHAFGGHHGFH0E??<@DGHGGCFGGF?DGGGFFGGGGGGGG.C/0BBF?FBBEGG0FFF.AA9;B9/E////99;;:.//:FFFFBBF//9 -@M01368:8:000000000-A3GHV:1:1110:16700:6843/1 -AAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCTGTCTCTTATACACATCTCCGAGCC -+ -BBBBBFFFFFFFGGGGGGGGGGHHHHGHHHHHHHHHHHHGGGGGHHHHHGGHGHGGGGGGHHHGFFGHHGGGGHHHHHHCGG@FGHFHFEGGGGGGHGGGGDGHHHHHHHHHHHHHHGHHGHGHHGGGGGCGHEHGHHHFHHHFF.DGHHHHHHGHHHHHHGFGHGCGHGHHHHHHHHGHHHCD?:A::FCDGEGFGFFBFGBFGGGF00CBFFFF/BFBFFFFAE.FBBFBFFFFB/BFFBFFF/ADCAD -@M01368:8:000000000-A3GHV:1:1110:18014:7856/1 -ATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGCAAAAAAAAAATAG -+ -AABBBFFFFFFFCGGGCGGGGGGHHHHHGFHHHHHHGHHHGGGGGFHHGHFGGHHHGGFGGGHHGGGGGHHGGGGHGGHHHGGGGGGHHGHGDGGGGGGGGB/A99FE//BBFFF.9/FF?DA=@9./9 -@M01368:8:000000000-A3GHV:1:1110:23303:11846/1 -ACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGTGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTC -+ -3ABB?FAB?DAAGGGFBGGBB5AFFFGFHHGGGEGHHHFHHF3EGFHHHHEFHHGGHHHGH3EFHHHHHBDGHHE?ECHGHGHHHHHHFHHHGHHHHHHHGGHGGFHHHFHHGFFHHFEGFHHHHHHEHHFDAGHHHH/BDDFGGH1FDBGHHHHHHHCGHHHFH?0CGCF>CC=@--AFBFFB?=BFDFFF//FDB;-9A-AD-.A.A=?-9-:B/AEB/BFF.A;DDFFFBBFFFF/BBFFFFBFF9B; -@1:A:M01368:8:000000000-A3GHV:1:1111:13195:14562/1_PolyA:1 -ATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACCTGTCTCTTATACACATCTCCGAGCCCACGAG -+ -ABAAAFFFFFFFGGGGGGGGGGGHHGG4FDFGFGGCGGGHGHG?EGHEGFFDHFHHHHGGFE@EHHGEEEGFCCEFFGFG/EHHHHHBFBGGHFHFHEHGHEFGGGED/EFDEFFEHHBGHHCFFFDFGGHHGFFFHHHHGFG>0<0DGGFHBGHFBDFGHGFFGGAGHHGCGBHFFHHHFFFBFGGGGF/:FFFCB9F9FF0FEFE?FF0BED-D.//BF9//BBFF/BFFFEFFFFED-DCFF?.:BF -@M01368:8:000000000-A3GHV:1:1111:14287:20817/1 -GCCAACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTCATCGCACCTACGTTCACTATTACAGGCGAACATACTTATTAAAGTGTGTTAATTAAT -+ ->>A3>AAAACFFFGFGFGGGGGHGHHHHHHHGGGGGHGHFFCGHHGBGHFHHHHAGHHHHEHHAGFFEHEAE?GHHHHEGFFFDGHGHBFFHFHHHGHGHGHBECG@@@EHGGEGCGGGGFFGGGG?BGGGGGG.-C.FEDCFFFEFFFAF/99BEFA.:9BFB/9FFFEFFFFFFEFFF/FBBFFFFFFFFFFFB//;.@@//;DFFFF//BBFFFBF;A;DF=>B//;9B//B/;/;9//9//;B/:B9 -@M01368:8:000000000-A3GHV:1:1111:23624:14516/1 -GTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGGGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAG -+ -3>3AAFFFFFAFGGGGGGGGBGHHHHHHGGHGGHEHGHADFHHHHEGGGHBGHFFHHEHHGHFHGG5FBEEGEGHHHGHHHEFDGHHFFGFGGHHGHHHGHBHGHFHFHHGGGGFCFBGFGE?GFBECEGDGHEHGDGCGHGC/DF@GHGFFCGGGGFFFFDD?@C@GFEC?-ADHFFHBGHFBHFG0CC//.;B0/CA.;AA.;@.AB/9BB/A/.AFFFE/BFFF///9B/FBDE9/;99/B/BFFEF/ -@M01368:8:000000000-A3GHV:1:1111:7437:24508/1 -ACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTGTCTCTTATACACATCTCCGAGCCCACGAG -+ -CBCCDCFFFFFFGGGGGGGGGGGGGGHHHHHHHHHHHHHHHHGGGFFEHGGHHHHHHHHHHHGHHGGGGGHHHHHHFHHHHGHHHHGHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHGHHGGGGGGGHGGGGGCGHHHHGHHGHHHHHHHEDHHGHGGGGGD;BEGGGFFGGGGC:CFGGGGGGGGGFGGGFFFGFFFFF;FFF;BFFFFFFFFFFFB:FFFFD=CAEEEF>- -@M01368:8:000000000-A3GHV:1:1111:8665:25542/1 -TCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTGTCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCG -+ -AA3AAFFFFFFFGGGFGFFGGGGHGH4AGGGFHHCGEE2FGHHHHHHHHHHGFHGF53FHEGGFAF5CFHHGGFGHFHHGGHHHCHHHHHHHFHHGH?EEEG1FFFEHHFHFGGHHGG?F4FBGHGHCHEF0//-9--;0CFGG?@BGDGFFFFEFBBBDA?FFBFFFF=B;-9.AA9EFFFF/;9BD.//;9/9/B:/:BFBFFBFFF/B:BFF//;/B/FFFBFF.A-BFFFBA/F//BFFFBF/;/A- -@1:A:M01368:8:000000000-A3GHV:1:1111:9655:13916/1_PolyA:1 -GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGCGGTGTCCACGCAATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTG -+ -1>>1AAFFFFFBGGFE1EEEEGGHGHHH2FGHHHFGFGHGCFGGGEFHGGEGGHHHHGHEFBFEGHHHGHHBEHHHHGHGFHHHGGGGHHEGGGGHHHGFHGHHHH1FHHG?G/AGF1GBFGEFFHF2FDD0FG--99@=---AAABA-BF/B?A@F-9BBFF//BB/9 -@M01368:8:000000000-A3GHV:1:1112:10272:7828/1 -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT -+ -AAABABBBBBBFGGGGGGGGGGHGHHGHGHHHHHHHHHGHHHGGHDGHHHGGGGHHHHHHHHHHHHHHHFHGHHHHGGHGGHHHFHHHHHCGHFFHHHHHHHHHHHHHGHGEFGAEEEEHHGHHGHGHHHHHHHGHHHHHHGHGHFFBC/BA?BGGGFFFFFFFFFFFFFFFC-@DDFFFFFFFEFAFFFFFFBFBFFFFFFFFFFFFFFFFBFF->@FFFFFFFFFFBFBFAD--.99;A?////:.9A. -@M01368:8:000000000-A3GHV:1:1112:9832:16531/1 -CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGGGCTGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCTTGAAGTAGGAACC -+ -AABABFFFFFFFF4GGGGEBGEFGHGGHGHGHHHGGHFBGFGGGGGHHEFHGEHFHFHEHHHHHGCGGGHFHHCFGHE@1EEEFFGB?EEEEHBGGGCHHGHHBEE@>EAHHFAEBDGGGCEFF?CEGFFHHHHHFHHHFHGHCGCAHHF<..C.D=0CD.GFHHHG.CAGHC:CHB0GGGFFB0:CFA.A-;9.;000CBBFFBFGA.9AA;A;EDDE.ABBFFFF9;BEFFF/BF//9/B//9:B//.. -@1:A:M01368:8:000000000-A3GHV:1:1112:9832:6701/1_PolyA:1 -CGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGG -+ -AABBBBFFFFFFGGGFGGGGGGHHHGGHGGHHHHHHHHHHHGHHGGGGGHHHHHHHHHHHHHHHHHHGGCGDHHHHFGGHHHGGGGGFGGHGGGGHHGGGGHHHHHHGGGGGGHHGGGGDGGFGHGGFCDCHHHHGHHHHGHHFAGFGGEHFGEG.C1>11F1D1DDFFFFGGG1FH3GHGHGHGHFHEC?EC0B1FBGFB1GHHH21AF2HH2FEGEHAE10AAE/>/FFHE?/E/?E1@EFFH?E@/EGGG//B11B//<@@C0F/<0AAAAFFFBFFFGGGGGGGGGGHHFHGHHHHGHHHHHHHHHHHGGGGHHHHFHHHHFHGHHHHGAFFEFHEGHHHHHHHHGHEHHGGFGGGHHHHHHFHHHHHGGHHHHGGGGGHFHHFF?HHGGFECEFFGHFFGFHGECDGHGBGFHGDF@@?CGFHCEGGGFD.CCC?EGHBHHHFHHFBCFFGEB/CEGGGGDAA.90C9CEBFGGBBF/9.9FBFFFBBFF//99FFFFEABF//99FFEFFFBFF -@1:A:M01368:8:000000000-A3GHV:1:1113:5741:16959/1_PolyA:1 -TAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG -+ -ABBBBFFFFFFFGGGFGGGGGGHHHGHHGGHBGHGAGFFCAFGHGFFGHHGFHHHHHGGGGGHGHHHHHHHHE3BFFHHHGG0EDF@GHFFGGGHGGGGGGGGGGGGGHHGGEEFHGFHHDDG@DGGGHHGDGGGGGHGG?CF?HHGHHHGHGHHHFFHGGGHHHHGGCD.;0A33>FFFFFF@FFFGGGGFGGD4EGGGGGHHGFFGHFGGHHHFEFHECFHHHEHGAGBCCGCCEGGHGHEGFBFHFHHHHGGGHFHGHEGGGFEGEGG??@DBGHGGC-@FFGHFHFHB-??DA-DD@9:BEBG@B?E?A.ABBFBFA??F.FF/BFFB/B9B/9;BF9FF9FFFFFFFFFFFFFF?BB?;9EE;-;DDB9//;B-B@;ABFFEFFFF/-.9A.;//9/BF./B/..9.9:...///// -@M01368:8:000000000-A3GHV:1:1114:14540:5315/1 -CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGGGGGCTATTTAGGTTTTT -+ -AABCCFFFFFFFGGGGGGGGGGHHHHHHHFHHHHGHHGHHGGGHGGHHHHHHHGHHHHHHGGGGGHHFHHHFGHHGGFHHHHHGGGGGHHHGHGGHHHGGGGGGHGHGGGGHHGGGGHHHHHEGDDFGFFFHHGGGGGCDAFCFGFDHHHHGGHGHHHHHHBCGEHHHHGGHG.ACGEHGG0CBFFF:A;BB0;09CGF00CFFFE0AA?//CFFFFFFFFFFFFFFFBEF;A.-=A--:BBFB90;;FE. -@1:A:M01368:8:000000000-A3GHV:1:1114:15066:16302/1_PolyA:1 -TAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAACGGTTGTTAATTAATTATTGCTTGTAGGAC -+ -BBBBAFFBDFBFBGGGGGFGGGBGFFFHGFHHGFFFHGHHHGHHHHFFHHHGHGC?AEFFHEFBFFFGHHHHH1FDGFFHGHGHFEGCGC-<>ABFFFFFFGGCGC1BBF1GHHHBHHHHGFHGH1A100AA/GGEHGFBAA1FFD2FHHHHECG2F1BB/E0FC/A>EE/FGGFGEF1EGBBEHHCGGGHBGEHFHE0B?E--;C?CCGGG@A@GBFBBBB09?@??A-AB--ABE@=-=-9AE9A;FFFFFE=9-//;//;9FF/////;;///9B///;/B////9FFBB;--A@-;/9;9-:-/;;FFFE9BF?@;-9-99/B9F/://///99/; -@M01368:8:000000000-A3GHV:1:1114:2404:13066/1 -TCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCTGTCTATTATACACATC -+ -CCCCCFFFFCFFGGGGGGGGGGHHHHHGHHHHHHHHHFFHHHHHGGGGHHHHHHHHFHHHHHHFGGHHGGHGGHHHHHHGHHFHHHHGGGGGGHHHHHHGHHHHHHHHHHGGGGGGGHH?FGHHHGGGGGGHHGGFGGHHGGGGHHHHHFGGGGFGHGHHGGGGGGGHGGGEGGHHGHHHHHHHHHGFBFFDA0FGGGFFGG0:EFGGGGGGGG;AEBF0B0BFFBFFFFFFFFFFFFFFFFFFFFFEFF0 diff --git a/tools/trim_galore/test-data/trimmed_polyA_reads_pair2.fastq.gz b/tools/trim_galore/test-data/trimmed_polyA_reads_pair2.fastq.gz new file mode 100644 index 0000000000..921d8fc5cf Binary files /dev/null and b/tools/trim_galore/test-data/trimmed_polyA_reads_pair2.fastq.gz differ diff --git a/tools/trim_galore/test-data/trimmed_polyA_reads_pair2.fastqsanger b/tools/trim_galore/test-data/trimmed_polyA_reads_pair2.fastqsanger deleted file mode 100644 index 490febc003..0000000000 --- a/tools/trim_galore/test-data/trimmed_polyA_reads_pair2.fastqsanger +++ /dev/null @@ -1,396 +0,0 @@ -@M01368:8:000000000-A3GHV:1:1101:6911:8255/1 -ATCTGGTTCCTACTTCAGGGCCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATT -+ -BCCCCFFFFFFFGGGGGGGGGGGHHHHGHGHHHHHHHHHGGGGGGHHHHGHHHHHHHHHHGHHHHHHGGHGGHHHGHHHHFHHGHHHHHHHHHGHEHEFFGHHEGGCEFGGFHHHBGHHGHHHHGHFHHHGHGHGHGGCDFDDACGGGGGGGAAFFFFFFFFFBAFFFFFB;FFFFFFADDFFFFFFFFFFEFFFFFFFFFFBFFFFFFFFFFFFFFEFFFFFFFFBFEFFFFEFE;DFFFDFBFF/9BFB -@M01368:8:000000000-A3GHV:1:1101:14518:9998/1 -GTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGAT -+ -AAAAAFFFFFFFGGGGGGGGGGHGGHHHHGHHHHHHHGCGHHHHHHHHHHHHHHHGGGGGHHHHHHHHHGHHGFHFE5BGEEHFGGGHHHHHHHHFBHHGGGGFHGHHFGHHHHGHHHHHHGEGGGGFHFHGEGHHGGCDGDGHGGGDGGHGGCGGGHGHHH/ACDG?.1FGCDCCGCA.CC@CDCHFHGFFGGGEBFGAB//CEFBFGG.:;D;;A0AFFFFFB..:@ABFF//;BFFFFFBF/9D:A// -@M01368:8:000000000-A3GHV:1:1101:18422:19051/1 -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG -+ -CCCCCFDDDDDFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHFHHHHGGGGHHHHHHHHHGHHHHHHHHHHHHGGHGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHGCGGGHHHHHHHHHHHHHHHHHHHHHHGFDHGFHCFGGGGFGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF;FFFFFFFFFFFFFFFFFFFFFFFFFFFFEFBFFFFFFFFFF:FFF. -@M01368:8:000000000-A3GHV:1:1101:25545:21098/1 -ATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATAAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGGCTTATTTAAGGGGAACGGGTGGGG -+ -33AA?DFD5BDFGGGFEBDGEGHEGHGEGHCEGGHHCHGHHFFHHGFGAGE53FF2FAFFGDE5FFFE5GFBFGAEE1GHHHGHHHEHE3FGHF@GEGEGGHHGG3FAGFFDE?EEE3GFEGFGFGGCG?GHHHFHGGGC@DHFFHD/AA@AAAAD2ADDFFGGGGGFGGHA?EAEFBEAGHFABFGG5FDF5DB1EEGAFDFB53FF5FH@G5FFEHGHEFHFFHBE333GF43GCGGGGE@0?BFGGB0B?FHGFDGGHHHBFFDEGGHGFFFDFE@<1>@FFFGHHHHFHEFGDABFFGG/@DCE---;--..;.../9 -@M01368:8:000000000-A3GHV:1:1103:25519:6301/1 -AAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGCATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATTTCGT -+ -3A3AAFFFFBFFGGG2CF?4FGHGHHCHGGHHBHGFFAGGHHFFHHCGHFEGCECEGHFFFHGHHGFGHFGDAFDGFFEEFEGGBECC/;>=EF?AEFFFF;9;=DAFFFFBBFFFBFFF//;BFB.:FBFFBB/BFFFF/BFFE?EFAFEF9 -@1:A:M01368:8:000000000-A3GHV:1:1104:11928:24197/1_PolyA:1 -ACGTAGGTGCGATAAATAATGGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGAT -+ -CDDCCFCFFFBCGGGGGGGGGGHHHGHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHGGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHGHHHHGHHGHHHHGGHGEEGGGGFHHFHHHGHGEHHHHHHHHHHHHHHHHHGGGGGGFGGGGGGGGFFGGGFGFGAEFEFGFEAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF@D -@M01368:8:000000000-A3GHV:1:1104:12288:20635/1 -CACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGCCGCAGCATCTGTCTTTGATTCCTGCCT -+ -ABA?AFBAB2>AFGGGGFFGD5GGHGFFHGHEGGGGHHHHFFHH5FHGHHHFFHEFHHHHHHGGHHHHHGFHGGHGGGGFHGGGFBHHHFGEGHHHHGHGGGGECEHHFHHGHHFFHFGGGHHHEGHDGHHGHGHFHHHECGCBCHHGFHHFHD1GFHEFHEF?GDCFFEFE0A@;@>BD/9BFBBBBFDFFFF99D=?>DDBFF-AFFEBB9BBEFEFAFFF//-@---;;FBBFBFB///;99999BA9 -@M01368:8:000000000-A3GHV:1:1104:13832:4398/1 -CACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGACAAAAAAAAACACACAACAACCAACACCCAGTAGACTAGTGCGTC -+ -AAABABCAFFFFGGGGGGGGGGHFHHHHHHHHHGFFHHGGHGGGGGHHHHHHHHHHFHHHHGHHHHHGGHGHGGHGGGGGGGHGFFGGGGDGGHHHHGGGGFGGGGGCFGGEGCDGGGGGGGGGGGGEGF?;BFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF/FFFFFFC.;BFEAFFFFF;@FFBFFFFEF?/FFFFE----:...:...9....9.9...:.9//9////;/9-.- -@M01368:8:000000000-A3GHV:1:1104:23670:4161/1 -ATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCC -+ -AAAABFFFFFFFGGGGGGGCGBBGGHHHHHHHHHGHHHHHHHHHHFGGHHHHHHGGFHHHHHHHGGGGGFHHHGHHHHHGHHHHGHHGGGHGGGGHHHHHGHHHHHHEGGGFHHHGFFDGEGHGHFHGHHGEGGGHHBGHGGHGHGGGGG/@FCDFDGFHGGGDFFHFGFFGGGGGHHHHGGGGGGGEGCDG?GGBFFFEE00BFFGABBBAFGGGEGEA.9FFF/./;BFBF./AAF/BFFBBF/F/BFB -@M01368:8:000000000-A3GHV:1:1104:24927:22253/1 -AGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTAT -+ -AAA?AFFF3DFFFGGBGFF3EAEEEHFHHFHHHGHHHHDGHHGAEFGHHHGFCHHGHHHBGHHHAEGGHGGFHFBFHHHHHHHHHHFHH1EEHEEHH1FFDEEFCGHBGHHFHEHHHHHH1FCGHHHHEEFGEEGFFCHHH11>GHHF/FG2DGGCGAGGHGGGG@@@BEFFF?@@@FFEFFFFFF?@@FB@BB@FFFE=;==---A9EFBBB/;E;A9B/B/FFFEF/B///;/;9/A/;/9BFFFBFBB -@M01368:8:000000000-A3GHV:1:1104:4705:7727/1 -CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG -+ -BBBBBFFBFFFFGGGGGGGGGGHHHGHHHGHHHHH6GHHHHFFGGGHHHHHHHHHHHHHHHGGFGGGEGGGHHHHHHHHHHGHE3FGGGGHHHHHFHHHHHHHHHHHHGGGGGHHFHFCGFHHGGGGGGGHHGGGGHHGGGGGGHHHHGGGADCHHHHGDGGGGGGGGC@DGFHEHHHHHHGHHHHGFGDEGFGFFGGGGCFGBEGFGGGFG;C.BFFFFFFFBFBFFFF/F?.AFFF/BFFEFFFFFEFF -@1:A:M01368:8:000000000-A3GHV:1:1104:7756:4776/1_PolyA:1 -GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTG -+ -CCCCCCFFFFFFGGGGGGGEEFHHHHGHGHHHHHHGGHHHGGGGGGHHGHHHHHGHHGHHHGGFHHHHHHHHGHHHFHHGHHGFGGGGHHHHHHHHGHGHFFHFHHHHHHGGHGFGFHHHGHG?FGHHHHHHHHHHHHHHGHGHHGHGGCDGFFHHHHGHHHHHGHHHGFHGHFHGHGHGH/@CABFFFFFFFACAFF?BBFFBFD-@-.D>FFAF.F.:9---;AADFFFFFBEFFF:9FFFFBBFFFF -@M01368:8:000000000-A3GHV:1:1105:11310:21761/1 -ATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTT -+ -CCCCBFFFFFFFGGGGGGGGGGGHHHHHHHHHHHHGHHHHHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHGHHHGHHHGHGHHGHHHHGGHGGG=FFGEHHHHHGHFGGHHGHHHHHFHHHHHHHGGHHHHHHHGHEHHGHHHHHGGGGF9AGE?DEGFFGGGGGGGGGGGGGGFFFF/9FFBFFFFFFFEFFFBFFFFFFFFFFFFEFFF0 -@M01368:8:000000000-A3GHV:1:1105:11578:14676/1 -ATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTC -+ -CDDDDFFFBFFCGGGGGGGGGGHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHFGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHHHHHHHHHGHHHHGGGGGGHGGHGHHHHHHH;CHHHHHHHHHHHHHHHGHGGGGFGGGFGGGGGGGGGGGGFGGGEGGFFFFFFFFFFFFFFFFFFFFFFFFF/FFFFFFFFFFFFFFFF0 -@M01368:8:000000000-A3GHV:1:1105:13575:19255/1 -TTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGTTCCCGTGAGTGGTTAATAGGGTGATAGGCCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG -+ -AA1A1DFFFFFBGG3A111FFAEGDDBGFDHF00F01BBA00BFGEHFBFFA1FABB0FAE?CFHF1BGFHFB0011F121E//>>1F10>B?EFHGCC/?/BFG/EEGFFEE//BCFDE0?E@@B/?11AAFFFFFEAGGGFGGAA6FHDGGCGFHFFHFHFHHHHHGDDDFFFHGFFHGBEGEGHHHFFFHGEEAEGHHHHHFHHHFHGHFEDGGEGBEAGEFHHFFHHHHHHGGHHHGHHGBGHHFHHFGHHHHG?CG?AFHH1FHHHGHFHHHF?DGHFFHCGAGEFHGCBBBB?BFFF?B-B.9/BFFFFFB=-AD?..@FFFF@@-@@?AFD.FFFBBEEBD9/:FFFFFBB/:FF9BF/BF.BBFF/: -@M01368:8:000000000-A3GHV:1:1105:21099:2895/1 -TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTT -+ -CBCBBFFFFFFFGGGGGGGGGGGGGFHHHHFHGHHHHHHFHHHHFGFHHHHHHGHHHHHHGHHEHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHHHHHHHFHHHHGHGHHHGGHHHHHGGHFFGHGCGGGHGHHGHHHFGHHFHH/FGGGHFGFEGGCFCCD;B@DFFFFFFFFFFFFFFFFFFFFFFFFF;F.BEFADFFFF.AEFFFFFFE;D.;:B00099:09BFBFFBFF.0:000BFFBF0 -@M01368:8:000000000-A3GHV:1:1105:26469:19245/1 -TTATGATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCAAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATG -+ -BAAAAFFFBFFFGGGGGGFFGGHHHHHHHHHHHHGGHHGHHHGGHHHHHHHHHHHDEGGHGHHHHHGEDHFHGFHGGHHGGHGGEHHHGHHGHHGHHHGGEGGGHGHGHHHHHHGHHHHGHGGGGGHFHGGGGHHGGGGCDGHHHGD>A@FFFFFFFGGGFEECFGGFFB5GBHH5DFH5GBEEAEFHFHHF3F3GHFHHHEF3GAEHBGFHFFEFFHHHHFFB31FGGEGFHGEHHHHHHHHHHHHHHHHHEEEEGHHHFFF3GF3FGF44BBGEHHHHGG2B/BGGHE;B=.9.;.BBFFF..;A;/B/;B//;;//BBF/ -@2:A:M01368:8:000000000-A3GHV:1:1106:22927:3863/1_PolyA:2 -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGACTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT -+ -A>ABAFA?D@AFGGGGFGGGFGFHHHFFH4BCBGHHHHGFFF3G5BF3322EEAFHHHHHHAB5GHFHEHFHHH3BEEHFGF5@13FDF3GDGFHHFGHHHGHHHGFGGHHFCG//?/EGHHHG3F4BFFGHHHEDGFHHB/B/?FBF<@-9?BDEFFF?B--?DFFFB9FA>B;A.BFDB?AB.--@;-.FFAEFFFF9AE;DDFFFFB/9BFFFFFFFB:BF/BB/BFF//FFF-=--.99=--.9/ -@1:A:M01368:8:000000000-A3GHV:1:1106:23625:4236/1_PolyA:1 -TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATT -+ -@?AAAFFBFBFFFGGFGFGGGEFCGGHHHHFGHGHFHGHHHHGGFGGFGHHGHGHHHHGHGFFHHGGGGGHHHHHHHHHBFHHEFHHHHHHGF?FAGHGFHHHGDGFHHGGGHGHFHHHGHHHGHHFHHGFFGFHHHGHHHHFFHHHHH/FGHHHHGHGHGDFFFE?FFF/--;;9/:BFFFBA///9----;B.//////;...---9-- -@M01368:8:000000000-A3GHV:1:1106:6455:17124/1 -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG -+ -BBBAAFABDBBFGGGGGGGGGGHHHGHHGHHHHHHHHHGHHGHHGHHHGHGGGEGHHHHHHHHHHHHHGHHHGHHHGGHGGHHHHHHHHHHHHHHHHHHGHHHHHHHHGHHHHHGGCFEHHHGFHHHHGHHFFH?GGHHHHGHGHGHHCFGGGGGGGFFFFFFFFFFFFFFFFFFFFADFA?.FEFAFFFEFFFFFFFFFFFFDABFBBFFFFFFFFEFFBFFFFFFFFFFF/FFBFFFFFFADE>BFFF9 -@1:A:M01368:8:000000000-A3GHV:1:1107:14451:26748/1_PolyA:1 -GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTG -+ -BABBBFFDABBBGGGGGGGGGGHGHHHHGAGHGGGGGHHHHHHHHGHHHHHGGGGGGFFHHHHHHHHHFHHHHHGGGGCHFHH3FEHHGCGGGDGHHHGFGGHHGGGGHHHHHHG/DGGGHHHHGGGGGCCHGG?CGG1GHHGHHHHBGHHHFHHGHHHHGGADAD00ECEGHEFHHFFAEFFFFFGGFBFBFFFGFFG.;B/99;BCFGGGGEFFFFFFEDA./:ADEB?FBFFFF;FFEBFBFFB./9 -@1:A:M01368:8:000000000-A3GHV:1:1107:18714:24788/1_PolyA:1 -GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTG -+ -BBBCCFFFDEDEGGGGGGGGGGHHHHHHGGHHGGHGGHHHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHCFGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHGHHHHHHHHHHHGHHHHGGEGEGCCEGGHHHHHHG:CAFFGGGGGGBFEFGGGGHGAFFGGFGGGGGGGEFFFFFFFFFEF/EDDFFFFFFFFFBFFFFFBBFFBBF -@M01368:8:000000000-A3GHV:1:1107:21355:8333/1 -ATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATG -+ -ABCBBFFFFFAFGGGGGGGGGGHHHHHHHHFHHHHHGGGGGHHHHHHFHHHGHHHHHHHGGGGGHHHHFFGHGHGGGGGGGHGGEGGGHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHGHHHFHHHHHHHHGHFFHHHHGGHGEGHHGHGFHHFHGHCEFHEGHGHHHGHHHGGGGGGCFFFFBFGGFGGGGFBG?FDGGGEGFFFFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFF.AA/9 -@M01368:8:000000000-A3GHV:1:1107:22864:15712/1 -AATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG -+ -BBBBBFBBFFFFGGGGCEGGGGHGHHDGFHHHHHHHHHHHHHCHHGHFGBGDFGE?GHHGHFBHHFHHHHHHHHHGHFGFGHGHHGGC@DCFGHHGGGGGGGHHHHHHGGGGGFGGGGFFGGGGFADGFEFFFFFFFFFFF9;FFFF/BFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFEAECDFFFFFFFFFBFFFFFFFFFFFFFFEFBFBFFF/FFFDF-;-9;E@>-9DFFF/FFBF;-.:;.9A// -@M01368:8:000000000-A3GHV:1:1107:24817:14233/1 -CTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACAT -+ -3AABABFFBFFFGCFGGGGGGGHHHFGCFGGGFFGHHCGHHHGHGHGHBFFHHHEDGGFGFGHHFFFHGGEGHFHHGHFHGFFHHHFGHHHFHACECEHHHBGFHGFHHHFHHF4GFHFHFHEHGGE/EG:A-CFGGFGADGGGGFGGGGDAAAADFA.-AFFECFDAAFFFFEFFFFBFF;D;BFFFB/;BFFFFFFFFFFFFFFBFFBF/;//;BFFFF?FFFFFFEDAFFFFFBF//:BFBACF--9/ -@M01368:8:000000000-A3GHV:1:1107:24830:14440/1 -AACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACC -+ -ABBBA@FFFFBBGGGGGGGGGGHGHHGHHGHHHGGHHHHHGHGHHGDHHHHHHAGGGGFHHGHHHHHHGHHHHHHHHHHHEGAFGFHFHHHHGEGHHHHHHHHHHHHHHHHHGGHHHGEFEGGHHGHHHHHGGHGFHH/?GHHHHGFGHGFHCGGBBDAFFFEFFFFFFFFFFFFFF=CDDADDFFFFEF=BFFFEFFFBFFFFEEBF./:FFFFFFFFFFFFFFFFFFFEFFFFFFFFFFF/BFBEFFC- -@M01368:8:000000000-A3GHV:1:1107:26478:23602/1 -GCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATCTGTCTCTTATACACCTCTC -+ -1AAAAF1B13BBBEGGGFGGGGGGHHDB1BGFHHHFGFGGG0F00BGBGFGGF1GBGB1BGCGAEE/FGFHGHFHHHHHBFF1B0FGGF//F//FGFGFFF/1@B1DFHHHGGEFEDHFDFAFF0B//1FDD1GFFHHDF?1BD>GF/GCAC0C@?@-;99BBB->@--;-B//99B--9-BF=A--A-:A----9AA9AFBFBBB?E@BBFFFFFFFBFFFFFBFFFBBFFF//B/////-;9 -@M01368:8:000000000-A3GHV:1:1108:11059:23181/1 -GTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGAT -+ -CCCCCFFFFFFFGGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHGHHGGGGHIIIHHHHHHHGHHHHGHHHGGHGGHHHHHHHHHHHHHHGGGGGHHGHHHHHHHHHHHHHHHGGGGGHHHHHFGHHHGGGGGGHHHGGGGGHGGGGHHHHHHGGGGGGHGGHEFGGGGGHGGG-ECHHHGGGGGGGGGFGFFGGGGFGGGCF?EFGG0;BFFFFB?9AAFFFFFFBF;BBFFBFFFFE?F/ -@M01368:8:000000000-A3GHV:1:1108:12233:13498/1 -TAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCCTGTCTCTTATACAC -+ -BCCBAFFBCCCCGGGGGGGGGGHHHHGGGGGHHHHHHGHGHBGFHHHHHGGGGGHHGGBFGHHGGGGGGGHHHGGGGHHGGGFHHHHHHGGGGGGHGHHGGGGGGGGGGGGGGHHHHFHHHGHGHHHHHHGHGHHGGFGGGDFFGGHHHGHGH/FHHHFHGHGHHGHHHGHHBAAAA3BDFFFFDGBFFEEGGGGHHGHFHHCFHGHGHHHGF3BFGFHGFEFHFHGH3EGFC1EEGBFGBHFGGFFHHHGHFHGHHGHGGHCEHEBCFF3ED3@GH4FDEG12FFHFFHG/G3FGA/@EFEFCG33FFGBBGHFHH/?BFBGFGFAGC2C/@-;@-;BFFEFADF?DFFFFFF?;AFFA;AD;..FEFFA--;9;D.9BFFBE?;.;///://///99//BB/9;F./B//BBFB9////99/ -@M01368:8:000000000-A3GHV:1:1109:25237:11722/1 -CCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATATCGTATGCCGT -+ -AABBBFFFFFBBF5BGGFGABGHGGAEFGFHHHHFHCHFHHBFGGGEHBGGHFFFFGFAEF5FHH?EGGF5AEG3?EBFHD?C/EFHFFEGEE?CFGGGHHFHB3EEFG?EEGHCFGGGGGGGECA?FFFDFBFGCG4EGFG??GACFGGHFHCFHCHBECDEFCEGHHHHHFFGGF3AFFHHFGEGCGHHGEHGFGHHEGG?EFHHH?EGGHHGGGGHHHHHHEGGGGGHFHHGFDDGGACFCC@?/FDDGHHHGHEFFHHH0FDHHFFEDCD.CFHHGDFGGGGGEFGC?EGHHHB0CGHHGCGHHHHBEHGHH.::ACA;FECFGGGGGFFFEFGGGGGGGFFBBBFGBFFFEFFFFBFFFFFFBBFBFFB9..AF?FFFFFFF9 -@M01368:8:000000000-A3GHV:1:1110:14751:26540/1 -TAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACTGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGTTGTGCACGCGATAGCATTGCGCGACGCTGGCGCCGGCGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTG -+ -AAA?1CFFFFADGEEGFGB1112DGDGHGFGHHCG/ECEBGGHDFGHHFHGHEB1BCGFFE2EGHHGHFFFHHBFFHEFCGGHHFHHHHH111BFFHGHEHBEHGGECEFHGFHFGGDBBGHHBHHHH0GH22FGHGFFFH1BFCG0F@FGG11@GDHFG1D/1FGFHH/FCG.>0><-<@CCFFG.CC-@C?C9FFF0C---;------;9----9-9@-AAFF9B9---9-/;B9BF/9BB//B/;/// -@M01368:8:000000000-A3GHV:1:1110:15519:10008/1 -CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACC -+ -BBBBBFFFFFFFGGGGGGCBGGE?GGGHHHHHGHHBEGGHHGGGGGHHHHHHHHHHHGGHHHHGFGGGGHGHGCFGHHHGGGCGGHGHGGGGGHFGGGHHHHHHGCEFGGHHHHGGGGGGGHGG/CEGHHHHGFHHHGFHHGFFHGHGHFGGEGFDDGGBCGHHGFHH/CHHHHGHGHF0CFGGGGGGGFFFFFBFFFFFFGFFGGF?DGEDEEFEFFFF?FFFFFFFFFFFFFFFBFFF.BFFFBFBBA; -@M01368:8:000000000-A3GHV:1:1110:16559:7507/1 -CATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAACTGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATC -+ -ABBBAFFFFFFFFGGGGGGGFGGGGAGHHGCHHGHHHHHHHHHHHGHHGHHHHHHHHHHHHHHHHFGGGHHHHHHHHBDHHHHHGGHFEEGHGGHHHHHHHGHHHEHFGGGGGGHHHF33FGHHG3GDDGH//EEGHHFFBF?GHHFCC@DGFCGGB?DGHAFGGHHGFH0E??<@DGHGGCFGGF?DGGGFFGGGGGGGG.C/0BBF?FBBEGG0FFF.AA9;B9/E////99;;:.//:FFFFBBF//9 -@M01368:8:000000000-A3GHV:1:1110:16700:6843/1 -AAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCTGTCTCTTATACACATCTCCGAGCC -+ -BBBBBFFFFFFFGGGGGGGGGGHHHHGHHHHHHHHHHHHGGGGGHHHHHGGHGHGGGGGGHHHGFFGHHGGGGHHHHHHCGG@FGHFHFEGGGGGGHGGGGDGHHHHHHHHHHHHHHGHHGHGHHGGGGGCGHEHGHHHFHHHFF.DGHHHHHHGHHHHHHGFGHGCGHGHHHHHHHHGHHHCD?:A::FCDGEGFGFFBFGBFGGGF00CBFFFF/BFBFFFFAE.FBBFBFFFFB/BFFBFFF/ADCAD -@M01368:8:000000000-A3GHV:1:1110:18014:7856/1 -ATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGCAAAAAAAAAATAG -+ -AABBBFFFFFFFCGGGCGGGGGGHHHHHGFHHHHHHGHHHGGGGGFHHGHFGGHHHGGFGGGHHGGGGGHHGGGGHGGHHHGGGGGGHHGHGDGGGGGGGGB/A99FE//BBFFF.9/FF?DA=@9./9 -@M01368:8:000000000-A3GHV:1:1110:23303:11846/1 -ACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGTGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTC -+ -3ABB?FAB?DAAGGGFBGGBB5AFFFGFHHGGGEGHHHFHHF3EGFHHHHEFHHGGHHHGH3EFHHHHHBDGHHE?ECHGHGHHHHHHFHHHGHHHHHHHGGHGGFHHHFHHGFFHHFEGFHHHHHHEHHFDAGHHHH/BDDFGGH1FDBGHHHHHHHCGHHHFH?0CGCF>CC=@--AFBFFB?=BFDFFF//FDB;-9A-AD-.A.A=?-9-:B/AEB/BFF.A;DDFFFBBFFFF/BBFFFFBFF9B; -@1:A:M01368:8:000000000-A3GHV:1:1111:13195:14562/1_PolyA:1 -ATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACCTGTCTCTTATACACATCTCCGAGCCCACGAG -+ -ABAAAFFFFFFFGGGGGGGGGGGHHGG4FDFGFGGCGGGHGHG?EGHEGFFDHFHHHHGGFE@EHHGEEEGFCCEFFGFG/EHHHHHBFBGGHFHFHEHGHEFGGGED/EFDEFFEHHBGHHCFFFDFGGHHGFFFHHHHGFG>0<0DGGFHBGHFBDFGHGFFGGAGHHGCGBHFFHHHFFFBFGGGGF/:FFFCB9F9FF0FEFE?FF0BED-D.//BF9//BBFF/BFFFEFFFFED-DCFF?.:BF -@M01368:8:000000000-A3GHV:1:1111:14287:20817/1 -GCCAACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTCATCGCACCTACGTTCACTATTACAGGCGAACATACTTATTAAAGTGTGTTAATTAAT -+ ->>A3>AAAACFFFGFGFGGGGGHGHHHHHHHGGGGGHGHFFCGHHGBGHFHHHHAGHHHHEHHAGFFEHEAE?GHHHHEGFFFDGHGHBFFHFHHHGHGHGHBECG@@@EHGGEGCGGGGFFGGGG?BGGGGGG.-C.FEDCFFFEFFFAF/99BEFA.:9BFB/9FFFEFFFFFFEFFF/FBBFFFFFFFFFFFB//;.@@//;DFFFF//BBFFFBF;A;DF=>B//;9B//B/;/;9//9//;B/:B9 -@M01368:8:000000000-A3GHV:1:1111:23624:14516/1 -GTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGGGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAG -+ -3>3AAFFFFFAFGGGGGGGGBGHHHHHHGGHGGHEHGHADFHHHHEGGGHBGHFFHHEHHGHFHGG5FBEEGEGHHHGHHHEFDGHHFFGFGGHHGHHHGHBHGHFHFHHGGGGFCFBGFGE?GFBECEGDGHEHGDGCGHGC/DF@GHGFFCGGGGFFFFDD?@C@GFEC?-ADHFFHBGHFBHFG0CC//.;B0/CA.;AA.;@.AB/9BB/A/.AFFFE/BFFF///9B/FBDE9/;99/B/BFFEF/ -@M01368:8:000000000-A3GHV:1:1111:7437:24508/1 -ACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTGTCTCTTATACACATCTCCGAGCCCACGAG -+ -CBCCDCFFFFFFGGGGGGGGGGGGGGHHHHHHHHHHHHHHHHGGGFFEHGGHHHHHHHHHHHGHHGGGGGHHHHHHFHHHHGHHHHGHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHGHHGGGGGGGHGGGGGCGHHHHGHHGHHHHHHHEDHHGHGGGGGD;BEGGGFFGGGGC:CFGGGGGGGGGFGGGFFFGFFFFF;FFF;BFFFFFFFFFFFB:FFFFD=CAEEEF>- -@M01368:8:000000000-A3GHV:1:1111:8665:25542/1 -TCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTGTCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCG -+ -AA3AAFFFFFFFGGGFGFFGGGGHGH4AGGGFHHCGEE2FGHHHHHHHHHHGFHGF53FHEGGFAF5CFHHGGFGHFHHGGHHHCHHHHHHHFHHGH?EEEG1FFFEHHFHFGGHHGG?F4FBGHGHCHEF0//-9--;0CFGG?@BGDGFFFFEFBBBDA?FFBFFFF=B;-9.AA9EFFFF/;9BD.//;9/9/B:/:BFBFFBFFF/B:BFF//;/B/FFFBFF.A-BFFFBA/F//BFFFBF/;/A- -@1:A:M01368:8:000000000-A3GHV:1:1111:9655:13916/1_PolyA:1 -GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGCGGTGTCCACGCAATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTG -+ -1>>1AAFFFFFBGGFE1EEEEGGHGHHH2FGHHHFGFGHGCFGGGEFHGGEGGHHHHGHEFBFEGHHHGHHBEHHHHGHGFHHHGGGGHHEGGGGHHHGFHGHHHH1FHHG?G/AGF1GBFGEFFHF2FDD0FG--99@=---AAABA-BF/B?A@F-9BBFF//BB/9 -@M01368:8:000000000-A3GHV:1:1112:10272:7828/1 -GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT -+ -AAABABBBBBBFGGGGGGGGGGHGHHGHGHHHHHHHHHGHHHGGHDGHHHGGGGHHHHHHHHHHHHHHHFHGHHHHGGHGGHHHFHHHHHCGHFFHHHHHHHHHHHHHGHGEFGAEEEEHHGHHGHGHHHHHHHGHHHHHHGHGHFFBC/BA?BGGGFFFFFFFFFFFFFFFC-@DDFFFFFFFEFAFFFFFFBFBFFFFFFFFFFFFFFFFBFF->@FFFFFFFFFFBFBFAD--.99;A?////:.9A. -@M01368:8:000000000-A3GHV:1:1112:9832:16531/1 -CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGGGCTGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCTTGAAGTAGGAACC -+ -AABABFFFFFFFF4GGGGEBGEFGHGGHGHGHHHGGHFBGFGGGGGHHEFHGEHFHFHEHHHHHGCGGGHFHHCFGHE@1EEEFFGB?EEEEHBGGGCHHGHHBEE@>EAHHFAEBDGGGCEFF?CEGFFHHHHHFHHHFHGHCGCAHHF<..C.D=0CD.GFHHHG.CAGHC:CHB0GGGFFB0:CFA.A-;9.;000CBBFFBFGA.9AA;A;EDDE.ABBFFFF9;BEFFF/BF//9/B//9:B//.. -@1:A:M01368:8:000000000-A3GHV:1:1112:9832:6701/1_PolyA:1 -CGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGG -+ -AABBBBFFFFFFGGGFGGGGGGHHHGGHGGHHHHHHHHHHHGHHGGGGGHHHHHHHHHHHHHHHHHHGGCGDHHHHFGGHHHGGGGGFGGHGGGGHHGGGGHHHHHHGGGGGGHHGGGGDGGFGHGGFCDCHHHHGHHHHGHHFAGFGGEHFGEG.C1>11F1D1DDFFFFGGG1FH3GHGHGHGHFHEC?EC0B1FBGFB1GHHH21AF2HH2FEGEHAE10AAE/>/FFHE?/E/?E1@EFFH?E@/EGGG//B11B//<@@C0F/<0AAAAFFFBFFFGGGGGGGGGGHHFHGHHHHGHHHHHHHHHHHGGGGHHHHFHHHHFHGHHHHGAFFEFHEGHHHHHHHHGHEHHGGFGGGHHHHHHFHHHHHGGHHHHGGGGGHFHHFF?HHGGFECEFFGHFFGFHGECDGHGBGFHGDF@@?CGFHCEGGGFD.CCC?EGHBHHHFHHFBCFFGEB/CEGGGGDAA.90C9CEBFGGBBF/9.9FBFFFBBFF//99FFFFEABF//99FFEFFFBFF -@1:A:M01368:8:000000000-A3GHV:1:1113:5741:16959/1_PolyA:1 -TAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG -+ -ABBBBFFFFFFFGGGFGGGGGGHHHGHHGGHBGHGAGFFCAFGHGFFGHHGFHHHHHGGGGGHGHHHHHHHHE3BFFHHHGG0EDF@GHFFGGGHGGGGGGGGGGGGGHHGGEEFHGFHHDDG@DGGGHHGDGGGGGHGG?CF?HHGHHHGHGHHHFFHGGGHHHHGGCD.;0A33>FFFFFF@FFFGGGGFGGD4EGGGGGHHGFFGHFGGHHHFEFHECFHHHEHGAGBCCGCCEGGHGHEGFBFHFHHHHGGGHFHGHEGGGFEGEGG??@DBGHGGC-@FFGHFHFHB-??DA-DD@9:BEBG@B?E?A.ABBFBFA??F.FF/BFFB/B9B/9;BF9FF9FFFFFFFFFFFFFF?BB?;9EE;-;DDB9//;B-B@;ABFFEFFFF/-.9A.;//9/BF./B/..9.9:...///// -@M01368:8:000000000-A3GHV:1:1114:14540:5315/1 -CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGGGGGCTATTTAGGTTTTT -+ -AABCCFFFFFFFGGGGGGGGGGHHHHHHHFHHHHGHHGHHGGGHGGHHHHHHHGHHHHHHGGGGGHHFHHHFGHHGGFHHHHHGGGGGHHHGHGGHHHGGGGGGHGHGGGGHHGGGGHHHHHEGDDFGFFFHHGGGGGCDAFCFGFDHHHHGGHGHHHHHHBCGEHHHHGGHG.ACGEHGG0CBFFF:A;BB0;09CGF00CFFFE0AA?//CFFFFFFFFFFFFFFFBEF;A.-=A--:BBFB90;;FE. -@1:A:M01368:8:000000000-A3GHV:1:1114:15066:16302/1_PolyA:1 -TAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAACGGTTGTTAATTAATTATTGCTTGTAGGAC -+ -BBBBAFFBDFBFBGGGGGFGGGBGFFFHGFHHGFFFHGHHHGHHHHFFHHHGHGC?AEFFHEFBFFFGHHHHH1FDGFFHGHGHFEGCGC-<>ABFFFFFFGGCGC1BBF1GHHHBHHHHGFHGH1A100AA/GGEHGFBAA1FFD2FHHHHECG2F1BB/E0FC/A>EE/FGGFGEF1EGBBEHHCGGGHBGEHFHE0B?E--;C?CCGGG@A@GBFBBBB09?@??A-AB--ABE@=-=-9AE9A;FFFFFE=9-//;//;9FF/////;;///9B///;/B////9FFBB;--A@-;/9;9-:-/;;FFFE9BF?@;-9-99/B9F/://///99/; -@M01368:8:000000000-A3GHV:1:1114:2404:13066/1 -TCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCTGTCTATTATACACATC -+ -CCCCCFFFFCFFGGGGGGGGGGHHHHHGHHHHHHHHHFFHHHHHGGGGHHHHHHHHFHHHHHHFGGHHGGHGGHHHHHHGHHFHHHHGGGGGGHHHHHHGHHHHHHHHHHGGGGGGGHH?FGHHHGGGGGGHHGGFGGHHGGGGHHHHHFGGGGFGHGHHGGGGGGGHGGGEGGHHGHHHHHHHHHGFBFFDA0FGGGFFGG0:EFGGGGGGGG;AEBF0B0BFFBFFFFFFFFFFFFFFFFFFFFFEFF0 diff --git a/tools/trim_galore/trim_galore.xml b/tools/trim_galore/trim_galore.xml index 5d7e6f43c8..7a628cb1ad 100644 --- a/tools/trim_galore/trim_galore.xml +++ b/tools/trim_galore/trim_galore.xml @@ -4,16 +4,14 @@ macros.xml - + trim_galore --version input_1_trimmed.fq.gz ; fi && - if [ -f input_1_val_1.fq.gz ] ; then mv input_1_val_1.fq.gz input_1_val_1.fq ; fi + if [ -f input_1_val_1.fq ] ; then gzip -n -c input_1_val_1.fq > input_1_val_1.fq.gz ; fi && - if [ -f input_2_val_2.fq.gz ] ; then mv input_2_val_2.fq.gz input_2_val_2.fq ; fi + if [ -f input_2_val_2.fq ] ; then gzip -n -c input_2_val_2.fq > input_2_val_2.fq.gz ; fi && - if [ -f input_1_unpaired_1.fq.gz ] ; then mv input_1_unpaired_1.fq.gz input_1_unpaired_1.fq ; fi + if [ -f input_1_unpaired_1.fq ] ; then gzip -n -c input_1_unpaired_1.fq > input_1_unpaired_1.fq.gz ; fi && - if [ -f input_2_unpaired_2.fq.gz ] ; then mv input_2_unpaired_2.fq.gz input_2_unpaired_2.fq ; fi + if [ -f input_2_unpaired_2.fq ] ; then gzip -n -c input_2_unpaired_2.fq > input_2_unpaired_2.fq.gz ; fi && - if [ -f input_1.clock_UMI.R1.fq.gz ] ; then mv input_1.clock_UMI.R1.fq.gz input_1.clock_UMI.R1.fq ; fi + if [ -f input_1.clock_UMI.R1.fq ] ; then gzip -n -c input_1.clock_UMI.R1.fq > input_1.clock_UMI.R1.fq.gz ; fi && - if [ -f input_2.clock_UMI.R2.fq.gz ] ; then mv input_2.clock_UMI.R2.fq.gz input_2.clock_UMI.R2.fq ; fi - + if [ -f input_2.clock_UMI.R2.fq ] ; then gzip -n -c input_2.clock_UMI.R2.fq > input_2.clock_UMI.R2.fq.gz ; fi + ## Rename hardtrimmed files #if $trimming.settingsType == 'custom' && - if [ -f input_1.${trimming.hardtrim5}bp_5prime.fq.gz ] ; then mv input_1.${trimming.hardtrim5}bp_5prime.fq.gz input_1_hardtrim.fq ; fi + if ls input_1.*bp_5prime.fq.gz >/dev/null 2>&1 ; then mv input_1.*bp_5prime.fq.gz input_1_hardtrim.fq.gz ; fi && - if [ -f input_2.${trimming.hardtrim5}bp_5prime.fq.gz ] ; then mv input_2.${trimming.hardtrim5}bp_5prime.fq.gz input_2_hardtrim.fq ; fi + if ls input_2.*bp_5prime.fq.gz >/dev/null 2>&1 ; then mv input_2.*bp_5prime.fq.gz input_2_hardtrim.fq.gz ; fi && - if [ -f input_1.${trimming.hardtrim3}bp_3prime.fq.gz ] ; then mv input_1.${trimming.hardtrim3}bp_3prime.fq.gz input_1_hardtrim.fq ; fi + if ls input_1.*bp_3prime.fq.gz >/dev/null 2>&1 ; then mv input_1.*bp_3prime.fq.gz input_1_hardtrim.fq.gz ; fi && - if [ -f input_2.${trimming.hardtrim3}bp_3prime.fq.gz ] ; then mv input_2.${trimming.hardtrim3}bp_3prime.fq.gz input_2_hardtrim.fq ; fi + if ls input_2.*bp_3prime.fq.gz >/dev/null 2>&1 ; then mv input_2.*bp_3prime.fq.gz input_2_hardtrim.fq.gz ; fi && - if [ -f input_1.${trimming.hardtrim5}bp_5prime.fq ] ; then mv input_1.${trimming.hardtrim5}bp_5prime.fq input_1_hardtrim.fq ; fi + if ls input_1.*bp_5prime.fq >/dev/null 2>&1 ; then gzip -n -c input_1.*bp_5prime.fq > input_1_hardtrim.fq.gz ; fi && - if [ -f input_2.${trimming.hardtrim5}bp_5prime.fq ] ; then mv input_2.${trimming.hardtrim5}bp_5prime.fq input_2_hardtrim.fq ; fi + if ls input_2.*bp_5prime.fq >/dev/null 2>&1 ; then gzip -n -c input_2.*bp_5prime.fq > input_2_hardtrim.fq.gz ; fi && - if [ -f input_1.${trimming.hardtrim3}bp_3prime.fq ] ; then mv input_1.${trimming.hardtrim3}bp_3prime.fq input_1_hardtrim.fq ; fi + if ls input_1.*bp_3prime.fq >/dev/null 2>&1 ; then gzip -n -c input_1.*bp_3prime.fq > input_1_hardtrim.fq.gz ; fi && - if [ -f input_2.${trimming.hardtrim3}bp_3prime.fq ] ; then mv input_2.${trimming.hardtrim3}bp_3prime.fq input_2_hardtrim.fq ; fi + if ls input_2.*bp_3prime.fq >/dev/null 2>&1 ; then gzip -n -c input_2.*bp_3prime.fq > input_2_hardtrim.fq.gz ; fi #end if ## Trim Galore! run is finished. Move the report files to the proper place @@ -193,7 +248,6 @@ && cat ./*_trimming_report.txt > '$report_file' #end if - && ls -lah ]]> @@ -204,9 +258,8 @@ - + - Instructs Trim Galore! to remove N bp from the 3' end of read 1 after adapter/quality trimming has been performed. This may remove some unwanted bias from the 3' end that is not directly related to adapter sequence or basecall quality. @@ -214,406 +267,406 @@ - - - + + + - - + + - - - + + - + - - - - - - - - - - +
+ + + + + + + + + + + + +
+ + + + + + + + + + + - - - + + + - + - - - - - -
-
- + + + + + + + + + + - - + + - + - - - - + + + + + + - + - + + + + + + + - - - - + + + + + + + + + + + + + +
- - + singlePaired['sPaired'] == "single" - trimming['hardtrim3'] == '' and trimming['hardtrim5'] == '' + trimming['settingsType'] == "default" or (trimming['hardtrim3'] == '' and trimming['hardtrim5'] == '') - - + singlePaired['sPaired'] == "single" trimming['settingsType'] == "custom" trimming['hardtrim3'] != '' or trimming['hardtrim5'] != '' - - - + + singlePaired['sPaired'] == "paired_collection" - trimming['hardtrim3'] == '' and trimming['hardtrim5'] == '' - trimming['clock'] == False + trimming['settingsType'] == "default" or (trimming['hardtrim3'] == '' and trimming['hardtrim5'] == '') + trimming['settingsType'] == "default" or trimming['clock'] == False - - - + + params['settingsType'] == "custom" params['retain_unpaired']['retain_unpaired_select'] == "retain_unpaired_output" singlePaired['sPaired'] == "paired_collection" - trimming['hardtrim3'] == '' and trimming['hardtrim5'] == '' + trimming['settingsType'] == "default" or (trimming['hardtrim3'] == '' and trimming['hardtrim5'] == '') - - - + + singlePaired['sPaired'] == "paired_collection" trimming['settingsType'] == "custom" trimming['hardtrim3'] or trimming['hardtrim5'] - - - + + singlePaired['sPaired'] == "paired_collection" trimming['settingsType'] == "custom" trimming['clock'] - - - + singlePaired['sPaired'] == "paired" - trimming['hardtrim3'] == '' and trimming['hardtrim5'] == '' - trimming['clock'] == False + trimming['settingsType'] == "default" or (trimming['hardtrim3'] == '' and trimming['hardtrim5'] == '') + trimming['settingsType'] == "default" or trimming['clock'] == False - - + singlePaired['sPaired'] == "paired" - trimming['hardtrim3'] == '' and trimming['hardtrim5'] == '' - trimming['clock'] == False + trimming['settingsType'] == "default" or (trimming['hardtrim3'] == '' and trimming['hardtrim5'] == '') + trimming['settingsType'] == "default" or trimming['clock'] == False - - + params['settingsType'] == "custom" params['retain_unpaired']['retain_unpaired_select'] == "retain_unpaired_output" singlePaired['sPaired'] == "paired" + trimming['settingsType'] == "default" or (trimming['hardtrim3'] == '' and trimming['hardtrim5'] == '') - - + params['settingsType'] == "custom" params['retain_unpaired']['retain_unpaired_select'] == "retain_unpaired_output" singlePaired['sPaired'] == "paired" + trimming['settingsType'] == "default" or (trimming['hardtrim3'] == '' and trimming['hardtrim5'] == '') - - + singlePaired['sPaired'] == "paired" trimming['settingsType'] == 'custom' trimming['hardtrim3'] or trimming['hardtrim5'] - - + singlePaired['sPaired'] == "paired" trimming['settingsType'] == 'custom' trimming['hardtrim3'] or trimming['hardtrim5'] - - + singlePaired['sPaired'] == "paired" trimming['settingsType'] == 'custom' trimming['clock'] - - + singlePaired['sPaired'] == "paired" trimming['settingsType'] == 'custom' trimming['clock'] - params['settingsType'] == "custom" params['report'] == True - - - + + - - + + - - + + - - + + - - + + - - + + - - - + + - + - + - - + + - + - + - - - + + - - + + - + - - + + - - + + - + - - - - + + + - - + + - - - + + + - - - + + + - - + + - - - + + + - - - + + - + - - + + - + - + - - + + - - - + + - - + + - + - - + + - + - + - - + + - - - + + - - - + + + - + - - + + - - - + + + - + - - + + - - - - + + + - + - - + + - - - + + + - + - - + + - +