Hi
I have 2 3D tif stacks of different channels obtained from fluorescence microscopy. Each stack is [512 , 512, 32] (32 is number of frames). Can someone please say how to use pysptools with this data to unmix the signals ?
We employ 4 fluorophores in the experiment. So, what is the number of end member components in this case ?
Hi
I have 2 3D tif stacks of different channels obtained from fluorescence microscopy. Each stack is [512 , 512, 32] (32 is number of frames). Can someone please say how to use pysptools with this data to unmix the signals ?
We employ 4 fluorophores in the experiment. So, what is the number of end member components in this case ?