diff --git a/awx/api/metadata.py b/awx/api/metadata.py
index 2d45f27f..f3546cef 100644
--- a/awx/api/metadata.py
+++ b/awx/api/metadata.py
@@ -153,7 +153,7 @@ def get_field_info(self, field):
                 pass
         if field.field_name == 'type':
             field_info['type'] = 'choice'
-        elif field.field_name in ('url', 'custom_virtualenv', 'token'):
+        elif field.field_name in ('url', 'token'):
             field_info['type'] = 'string'
         elif field.field_name in ('related', 'summary_fields'):
             field_info['type'] = 'object'
diff --git a/awx/api/serializers.py b/awx/api/serializers.py
index 7dafc59f..dc83d349 100644
--- a/awx/api/serializers.py
+++ b/awx/api/serializers.py
@@ -1321,8 +1321,8 @@ class OrganizationSerializer(BaseSerializer):
 
     class Meta:
         model = Organization
-        fields = ('*', 'max_hosts', 'custom_virtualenv', 'default_environment')
-        read_only_fields = ('*', 'custom_virtualenv')
+        fields = ('*', 'max_hosts', 'default_environment')
+        read_only_fields = ('*',)
 
     def get_related(self, obj):
         res = super(OrganizationSerializer, self).get_related(obj)
@@ -1490,14 +1490,13 @@ class Meta:
             'scm_update_on_launch',
             'scm_update_cache_timeout',
             'allow_override',
-            'custom_virtualenv',
             'default_environment',
             'signature_validation_credential',
         ) + (
             'last_update_failed',
             'last_updated',
         )  # Backwards compatibility
-        read_only_fields = ('*', 'custom_virtualenv')
+        read_only_fields = ('*',)
 
     def get_related(self, obj):
         res = super(ProjectSerializer, self).get_related(obj)
@@ -2359,12 +2358,11 @@ class Meta:
             'host_filter',
             'overwrite',
             'overwrite_vars',
-            'custom_virtualenv',
             'timeout',
             'verbosity',
             'limit',
         )
-        read_only_fields = ('*', 'custom_virtualenv')
+        read_only_fields = ('*',)
 
     def get_related(self, obj):
         res = super(InventorySourceOptionsSerializer, self).get_related(obj)
@@ -2568,8 +2566,6 @@ def validate(self, attrs):
 
 
 class InventoryUpdateSerializer(UnifiedJobSerializer, InventorySourceOptionsSerializer):
-    custom_virtualenv = serializers.ReadOnlyField()
-
     class Meta:
         model = InventoryUpdate
         fields = (
@@ -2579,7 +2575,6 @@ class Meta:
             'license_error',
             'org_host_limit_error',
             'source_project_update',
-            'custom_virtualenv',
             'instance_group',
             'scm_revision',
         )
@@ -3288,13 +3283,12 @@ class Meta:
             'become_enabled',
             'diff_mode',
             'allow_simultaneous',
-            'custom_virtualenv',
             'job_slice_count',
             'webhook_service',
             'webhook_credential',
             'prevent_instance_group_fallback',
         )
-        read_only_fields = ('*', 'custom_virtualenv')
+        read_only_fields = ('*',)
 
     def get_related(self, obj):
         res = super(JobTemplateSerializer, self).get_related(obj)
@@ -3461,11 +3455,10 @@ def get_summary_fields(self, obj):
 
 class JobDetailSerializer(JobSerializer):
     playbook_counts = serializers.SerializerMethodField(help_text=_('A count of all plays and tasks for the job run.'))
-    custom_virtualenv = serializers.ReadOnlyField()
 
     class Meta:
         model = Job
-        fields = ('*', 'host_status_counts', 'playbook_counts', 'custom_virtualenv')
+        fields = ('*', 'host_status_counts', 'playbook_counts')
 
     def get_playbook_counts(self, obj):
         task_count = obj.get_event_queryset().filter(event='playbook_on_task_start').count()
diff --git a/awx/api/templates/api/api_v2_config_view.md b/awx/api/templates/api/api_v2_config_view.md
index 725669bd..4d2eb7a1 100644
--- a/awx/api/templates/api/api_v2_config_view.md
+++ b/awx/api/templates/api/api_v2_config_view.md
@@ -11,8 +11,7 @@ the following fields (some fields may not be visible to all users):
 * `time_zone`: The configured time zone for the server.
 * `license_info`: Information about the current license.
 * `version`: Version of Ansible Tower package installed.
-* `custom_virtualenvs`: Deprecated venv locations from before migration to
-  execution environments. Export tooling is in `awx-manage` commands.
+
 * `eula`: The current End-User License Agreement
 {% endifmeth %}
 
diff --git a/awx/api/views/root.py b/awx/api/views/root.py
index 65f3e0e1..f4d5a1c4 100644
--- a/awx/api/views/root.py
+++ b/awx/api/views/root.py
@@ -25,11 +25,11 @@
 from awx.conf.registry import settings_registry
 from awx.main.analytics import all_collectors
 from awx.main.ha import is_ha_environment
-from awx.main.utils import get_awx_version, get_custom_venv_choices
+from awx.main.utils import get_awx_version
 from awx.main.utils.licensing import validate_entitlement_manifest
 from awx.api.versioning import reverse, drf_reverse
 from awx.main.constants import PRIVILEGE_ESCALATION_METHODS
-from awx.main.models import Project, Organization, Instance, InstanceGroup, JobTemplate
+from awx.main.models import Project, Organization, Instance, InstanceGroup
 from awx.main.utils import set_environ
 from awx.main.utils.licensing import get_licenser
 
@@ -314,11 +314,8 @@ def get(self, request, format=None):
                 dict(
                     project_base_dir=settings.PROJECTS_ROOT,
                     project_local_paths=Project.get_local_path_choices(),
-                    custom_virtualenvs=get_custom_venv_choices(),
                 )
             )
-        elif JobTemplate.accessible_objects(request.user, 'admin_role').exists():
-            data['custom_virtualenvs'] = get_custom_venv_choices()
 
         return Response(data)
 
diff --git a/awx/main/access.py b/awx/main/access.py
index c768e74c..30238e05 100644
--- a/awx/main/access.py
+++ b/awx/main/access.py
@@ -1664,7 +1664,6 @@ def changes_are_non_sensitive(self, obj, data):
             'ask_inventory_on_launch',
             'ask_credential_on_launch',
             'survey_enabled',
-            'custom_virtualenv',
             'diff_mode',
             'timeout',
             'job_slice_count',
diff --git a/awx/main/conf.py b/awx/main/conf.py
index 7c9d1f9e..e8cb2c1e 100644
--- a/awx/main/conf.py
+++ b/awx/main/conf.py
@@ -222,15 +222,6 @@
     category_slug='system',
 )
 
-register(
-    'CUSTOM_VENV_PATHS',
-    field_class=fields.StringListPathField,
-    label=_('Custom virtual environment paths'),
-    help_text=_('Paths where Tower will look for custom virtual environments (in addition to /var/lib/awx/venv/). Enter one path per line.'),
-    category=_('System'),
-    category_slug='system',
-    default=[],
-)
 
 register(
     'AD_HOC_COMMANDS',
diff --git a/awx/main/management/commands/custom_venv_associations.py b/awx/main/management/commands/custom_venv_associations.py
deleted file mode 100644
index 736dfe25..00000000
--- a/awx/main/management/commands/custom_venv_associations.py
+++ /dev/null
@@ -1,59 +0,0 @@
-# Copyright (c) 2021 Ansible, Inc.
-# All Rights Reserved
-
-from django.core.management.base import BaseCommand
-from awx.main.utils.common import get_custom_venv_choices
-from awx.main.models import Organization, InventorySource, JobTemplate, Project
-import yaml
-
-
-class Command(BaseCommand):
-    """Returns the pip freeze from the path passed in the argument"""
-
-    def add_arguments(self, parser):
-        parser.add_argument(
-            'path',
-            type=str,
-            nargs=1,
-            default='',
-            help='run this with a path to a virtual environment as an argument to see the associated Job Templates, Organizations, Projects, and Inventory Sources.',
-        )
-        parser.add_argument('-q', action='store_true', help='run with -q to output only the results of the query.')
-
-    def handle(self, *args, **options):
-        # look organiztions and unified job templates (which include JTs, workflows, and Inventory updates)
-        super(Command, self).__init__()
-        results = {}
-        path = options.get('path')
-        if path:
-            all_venvs = get_custom_venv_choices()
-            if path[0] in all_venvs:  # verify this is a valid path
-                path = path[0]
-                orgs = [{"name": org.name, "id": org.id} for org in Organization.objects.filter(custom_virtualenv=path)]
-                jts = [{"name": jt.name, "id": jt.id} for jt in JobTemplate.objects.filter(custom_virtualenv=path)]
-                proj = [{"name": proj.name, "id": proj.id} for proj in Project.objects.filter(custom_virtualenv=path)]
-                invsrc = [{"name": inv.name, "id": inv.id} for inv in InventorySource.objects.filter(custom_virtualenv=path)]
-                results["organizations"] = orgs
-                results["job_templates"] = jts
-                results["projects"] = proj
-                results["inventory_sources"] = invsrc
-                if not options.get('q'):
-                    msg = [
-                        '# Virtual Environments Associations:',
-                        yaml.dump(results),
-                        '- To list all (now deprecated) custom virtual environments run:',
-                        'awx-manage list_custom_venvs',
-                        '',
-                        '- To export the contents of a (deprecated) virtual environment, run the following command while supplying the path as an argument:',
-                        'awx-manage export_custom_venv /path/to/venv',
-                        '',
-                        '- Run these commands with `-q` to remove tool tips.',
-                        '',
-                    ]
-                    print('\n'.join(msg))
-                else:
-                    print(yaml.dump(results))
-
-            else:
-                print('\n', '# Incorrect path, verify your path is from the following list:')
-                print('\n'.join(all_venvs), '\n')
diff --git a/awx/main/management/commands/export_custom_venv.py b/awx/main/management/commands/export_custom_venv.py
deleted file mode 100644
index 82065b78..00000000
--- a/awx/main/management/commands/export_custom_venv.py
+++ /dev/null
@@ -1,48 +0,0 @@
-# Copyright (c) 2021 Ansible, Inc.
-# All Rights Reserved
-
-from awx.main.utils.common import get_custom_venv_pip_freeze, get_custom_venv_choices
-from django.core.management.base import BaseCommand
-
-
-class Command(BaseCommand):
-    """Returns the pip freeze from the path passed in the argument"""
-
-    def add_arguments(self, parser):
-        parser.add_argument(
-            'path',
-            type=str,
-            nargs=1,
-            default='',
-            help='run this with a path to a virtual environment as an argument to see the pip freeze data',
-        )
-        parser.add_argument('-q', action='store_true', help='run with -q to output only the results of the query.')
-
-    def handle(self, *args, **options):
-        super(Command, self).__init__()
-        if options.get('path'):
-            path = options.get('path')
-            all_venvs = get_custom_venv_choices()
-            if path[0] in all_venvs:
-                pip_data = get_custom_venv_pip_freeze(options.get('path')[0])
-                if pip_data:
-                    if not options.get('q'):
-                        msg = [
-                            '# Virtual environment contents:',
-                            pip_data,
-                            '- To list all (now deprecated) custom virtual environments run:',
-                            'awx-manage list_custom_venvs',
-                            '',
-                            '- To view the connections a (deprecated) virtual environment had in the database, run the following command while supplying the path as an argument:',
-                            'awx-manage custom_venv_associations /path/to/venv',
-                            '',
-                            '- Run these commands with `-q` to remove tool tips.',
-                            '',
-                        ]
-                        print('\n'.join(msg))
-                    else:
-                        print(pip_data)
-
-            else:
-                print('\n', '# Incorrect path, verify your path is from the following list:')
-                print('\n'.join(all_venvs))
diff --git a/awx/main/management/commands/list_custom_venvs.py b/awx/main/management/commands/list_custom_venvs.py
deleted file mode 100644
index 0df98c47..00000000
--- a/awx/main/management/commands/list_custom_venvs.py
+++ /dev/null
@@ -1,43 +0,0 @@
-# Copyright (c) 2021 Ansible, Inc.
-# All Rights Reserved
-import sys
-
-from awx.main.utils.common import get_custom_venv_choices
-from django.core.management.base import BaseCommand
-from django.conf import settings
-
-
-class Command(BaseCommand):
-    """Returns a list of custom venv paths from the path passed in the argument"""
-
-    def add_arguments(self, parser):
-        parser.add_argument('-q', action='store_true', help='run with -q to output only the results of the query.')
-
-    def handle(self, *args, **options):
-        super(Command, self).__init__()
-        venvs = get_custom_venv_choices()
-        if venvs:
-            if not options.get('q'):
-                msg = [
-                    '# Discovered Virtual Environments:',
-                    '\n'.join(venvs),
-                    '',
-                    '- To export the contents of a (deprecated) virtual environment, run the following command while supplying the path as an argument:',
-                    'awx-manage export_custom_venv /path/to/venv',
-                    '',
-                    '- To view the connections a (deprecated) virtual environment had in the database, run the following command while supplying the path as an argument:',
-                    'awx-manage custom_venv_associations /path/to/venv',
-                    '',
-                    '- Run these commands with `-q` to remove tool tips.',
-                    '',
-                ]
-                print('\n'.join(msg))
-            else:
-                print('\n'.join(venvs), '\n')
-        else:
-            msg = ["No custom virtual environments detected in:", settings.BASE_VENV_PATH]
-
-            for path in settings.CUSTOM_VENV_PATHS:
-                msg.append(path)
-
-            print('\n'.join(msg), file=sys.stderr)
diff --git a/awx/main/migrations/0195_remove_custom_virtualenv.py b/awx/main/migrations/0195_remove_custom_virtualenv.py
new file mode 100644
index 00000000..a3936693
--- /dev/null
+++ b/awx/main/migrations/0195_remove_custom_virtualenv.py
@@ -0,0 +1,37 @@
+# -*- coding: utf-8 -*-
+# Generated for custom venv removal
+
+from django.db import migrations
+
+
+class Migration(migrations.Migration):
+    dependencies = [
+        ('main', '0194_add_github_app_credential'),
+    ]
+
+    operations = [
+        migrations.RemoveField(
+            model_name='inventorysource',
+            name='custom_virtualenv',
+        ),
+        migrations.RemoveField(
+            model_name='inventoryupdate',
+            name='custom_virtualenv',
+        ),
+        migrations.RemoveField(
+            model_name='job',
+            name='custom_virtualenv',
+        ),
+        migrations.RemoveField(
+            model_name='jobtemplate',
+            name='custom_virtualenv',
+        ),
+        migrations.RemoveField(
+            model_name='organization',
+            name='custom_virtualenv',
+        ),
+        migrations.RemoveField(
+            model_name='project',
+            name='custom_virtualenv',
+        ),
+    ]
diff --git a/awx/main/models/__init__.py b/awx/main/models/__init__.py
index 3148f44f..fadff9fd 100644
--- a/awx/main/models/__init__.py
+++ b/awx/main/models/__init__.py
@@ -65,7 +65,6 @@
     ROLE_SINGLETON_SYSTEM_AUDITOR,
 )
 from awx.main.models.mixins import (  # noqa
-    CustomVirtualEnvMixin,
     ExecutionEnvironmentMixin,
     ResourceMixin,
     SurveyJobMixin,
diff --git a/awx/main/models/inventory.py b/awx/main/models/inventory.py
index bf7b86c7..1fe9af0c 100644
--- a/awx/main/models/inventory.py
+++ b/awx/main/models/inventory.py
@@ -47,7 +47,6 @@
     ResourceMixin,
     TaskManagerInventoryUpdateMixin,
     RelatedJobsMixin,
-    CustomVirtualEnvMixin,
 )
 from awx.main.models.notifications import (
     NotificationTemplate,
@@ -1093,7 +1092,7 @@ def credential(self):
     source_vars_dict = VarsDictProperty('source_vars')
 
 
-class InventorySource(UnifiedJobTemplate, InventorySourceOptions, CustomVirtualEnvMixin, RelatedJobsMixin):
+class InventorySource(UnifiedJobTemplate, InventorySourceOptions, RelatedJobsMixin):
     SOFT_UNIQUE_TOGETHER = [('polymorphic_ctype', 'name', 'inventory')]
 
     class Meta:
@@ -1269,7 +1268,7 @@ def _get_related_jobs(self):
         return InventoryUpdate.objects.filter(inventory_source=self)
 
 
-class InventoryUpdate(UnifiedJob, InventorySourceOptions, JobNotificationMixin, TaskManagerInventoryUpdateMixin, CustomVirtualEnvMixin):
+class InventoryUpdate(UnifiedJob, InventorySourceOptions, JobNotificationMixin, TaskManagerInventoryUpdateMixin):
     """
     Internal job for tracking inventory updates from external sources.
     """
diff --git a/awx/main/models/jobs.py b/awx/main/models/jobs.py
index 7bd52d44..f2f08c73 100644
--- a/awx/main/models/jobs.py
+++ b/awx/main/models/jobs.py
@@ -47,7 +47,6 @@
     SurveyJobTemplateMixin,
     SurveyJobMixin,
     TaskManagerJobMixin,
-    CustomVirtualEnvMixin,
     RelatedJobsMixin,
     WebhookMixin,
     WebhookTemplateMixin,
@@ -192,7 +191,7 @@ def passwords_needed_to_start(self):
         return needed
 
 
-class JobTemplate(UnifiedJobTemplate, JobOptions, SurveyJobTemplateMixin, ResourceMixin, CustomVirtualEnvMixin, RelatedJobsMixin, WebhookTemplateMixin):
+class JobTemplate(UnifiedJobTemplate, JobOptions, SurveyJobTemplateMixin, ResourceMixin, RelatedJobsMixin, WebhookTemplateMixin):
     """
     A job template is a reusable job definition for applying a project (with
     playbook) to an inventory source with a given credential.
@@ -553,7 +552,7 @@ def _get_related_jobs(self):
         return UnifiedJob.objects.filter(unified_job_template=self)
 
 
-class Job(UnifiedJob, JobOptions, SurveyJobMixin, JobNotificationMixin, TaskManagerJobMixin, CustomVirtualEnvMixin, WebhookMixin):
+class Job(UnifiedJob, JobOptions, SurveyJobMixin, JobNotificationMixin, TaskManagerJobMixin, WebhookMixin):
     """
     A job applies a project (with playbook) to an inventory source with a given
     credential.  It represents a single invocation of ansible-playbook with the
diff --git a/awx/main/models/mixins.py b/awx/main/models/mixins.py
index 6a389b3c..e8a837d8 100644
--- a/awx/main/models/mixins.py
+++ b/awx/main/models/mixins.py
@@ -2,7 +2,6 @@
 from copy import copy, deepcopy
 import json
 import logging
-import os
 
 import requests
 
@@ -10,7 +9,6 @@
 from django.apps import apps
 from django.conf import settings
 from django.contrib.contenttypes.models import ContentType
-from django.core.exceptions import ValidationError
 from django.db import models
 from django.db.models.query import QuerySet
 from django.utils.crypto import get_random_string
@@ -20,7 +18,7 @@
 
 # AWX
 from awx.main.models.rbac import Role, RoleAncestorEntry
-from awx.main.utils import parse_yaml_or_json, get_custom_venv_choices, get_licenser, polymorphic
+from awx.main.utils import parse_yaml_or_json, get_licenser, polymorphic
 from awx.main.utils.execution_environments import get_default_execution_environment
 from awx.main.utils.encryption import decrypt_value, get_encryption_key, is_encrypted
 from awx.main.utils.polymorphic import build_polymorphic_ctypes_map
@@ -40,7 +38,6 @@
     'TaskManagerProjectUpdateMixin',
     'TaskManagerInventoryUpdateMixin',
     'ExecutionEnvironmentMixin',
-    'CustomVirtualEnvMixin',
 ]
 
 
@@ -513,23 +510,6 @@ def resolve_execution_environment(self):
         return get_default_execution_environment()
 
 
-class CustomVirtualEnvMixin(models.Model):
-    class Meta:
-        abstract = True
-
-    custom_virtualenv = models.CharField(
-        blank=True, null=True, default=None, max_length=100, help_text=_('Local absolute file path containing a custom Python virtualenv to use')
-    )
-
-    def clean_custom_virtualenv(self):
-        value = self.custom_virtualenv
-        if value and os.path.join(value, '') not in get_custom_venv_choices():
-            raise ValidationError(_('{} is not a valid virtualenv in {}').format(value, settings.BASE_VENV_PATH))
-        if value:
-            return os.path.join(value, '')
-        return None
-
-
 class RelatedJobsMixin(object):
     """
     This method is intended to be overwritten.
diff --git a/awx/main/models/notifications.py b/awx/main/models/notifications.py
index 77f8032c..87db1834 100644
--- a/awx/main/models/notifications.py
+++ b/awx/main/models/notifications.py
@@ -280,7 +280,6 @@ class JobNotificationMixin(object):
         'diff_mode',
         'job_slice_number',
         'job_slice_count',
-        'custom_virtualenv',
         'approval_status',
         'approval_node_name',
         'workflow_url',
@@ -327,7 +326,6 @@ def context_stub(cls):
                 'artifacts': {},
                 'controller_node': 'foo_controller',
                 'created': datetime.datetime(2018, 11, 13, 6, 4, 0, 0, tzinfo=datetime.timezone.utc),
-                'custom_virtualenv': 'my_venv',
                 'description': 'Sample job description',
                 'diff_mode': False,
                 'elapsed': 0.403018,
diff --git a/awx/main/models/organization.py b/awx/main/models/organization.py
index 5e90c51a..7b97f5db 100644
--- a/awx/main/models/organization.py
+++ b/awx/main/models/organization.py
@@ -20,12 +20,12 @@
     ROLE_SINGLETON_SYSTEM_AUDITOR,
 )
 from awx.main.models.unified_jobs import UnifiedJob
-from awx.main.models.mixins import ResourceMixin, CustomVirtualEnvMixin, RelatedJobsMixin
+from awx.main.models.mixins import ResourceMixin, RelatedJobsMixin
 
 __all__ = ['Organization', 'Team', 'Profile', 'UserSessionMembership']
 
 
-class Organization(CommonModel, NotificationFieldsModel, ResourceMixin, CustomVirtualEnvMixin, RelatedJobsMixin):
+class Organization(CommonModel, NotificationFieldsModel, ResourceMixin, RelatedJobsMixin):
     """
     An organization is the basic unit of multi-tenancy divisions
     """
diff --git a/awx/main/models/projects.py b/awx/main/models/projects.py
index e81fd578..0879df32 100644
--- a/awx/main/models/projects.py
+++ b/awx/main/models/projects.py
@@ -32,7 +32,7 @@
     UnifiedJobTemplate,
 )
 from awx.main.models.jobs import Job
-from awx.main.models.mixins import ResourceMixin, TaskManagerProjectUpdateMixin, CustomVirtualEnvMixin, RelatedJobsMixin
+from awx.main.models.mixins import ResourceMixin, TaskManagerProjectUpdateMixin, RelatedJobsMixin
 from awx.main.utils import update_scm_url, polymorphic
 from awx.main.utils.ansible import skip_directory, could_be_inventory, could_be_playbook
 from awx.main.utils.execution_environments import get_control_plane_execution_environment
@@ -249,7 +249,7 @@ def get_lock_file(self):
         return proj_path + '.lock'
 
 
-class Project(UnifiedJobTemplate, ProjectOptions, ResourceMixin, CustomVirtualEnvMixin, RelatedJobsMixin):
+class Project(UnifiedJobTemplate, ProjectOptions, ResourceMixin, RelatedJobsMixin):
     """
     A project represents a playbook git repo that can access a set of inventories
     """
diff --git a/awx/main/tests/functional/models/test_notifications.py b/awx/main/tests/functional/models/test_notifications.py
index 2c1d6022..39fb6fe5 100644
--- a/awx/main/tests/functional/models/test_notifications.py
+++ b/awx/main/tests/functional/models/test_notifications.py
@@ -14,7 +14,6 @@ class TestJobNotificationMixin(object):
         'job': {
             'allow_simultaneous': bool,
             'artifacts': {},
-            'custom_virtualenv': str,
             'controller_node': str,
             'created': datetime.datetime,
             'description': str,
diff --git a/awx/main/utils/common.py b/awx/main/utils/common.py
index 81c64066..915314a3 100644
--- a/awx/main/utils/common.py
+++ b/awx/main/utils/common.py
@@ -10,7 +10,6 @@
 import time
 import psycopg
 import os
-import subprocess
 import re
 import stat
 import sys
@@ -80,7 +79,6 @@
     'has_model_field_prefetched',
     'set_environ',
     'IllegalArgumentError',
-    'get_custom_venv_choices',
     'ScheduleTaskManager',
     'ScheduleDependencyManager',
     'ScheduleWorkflowManager',
@@ -1011,35 +1009,6 @@ def get_current_apps():
     return current_apps
 
 
-def get_custom_venv_choices():
-    from django.conf import settings
-
-    all_venv_paths = settings.CUSTOM_VENV_PATHS + [settings.BASE_VENV_PATH]
-    custom_venv_choices = []
-
-    for venv_path in all_venv_paths:
-        if os.path.exists(venv_path):
-            for d in os.listdir(venv_path):
-                if venv_path == settings.BASE_VENV_PATH and d == 'awx':
-                    continue
-
-                if os.path.exists(os.path.join(venv_path, d, 'bin', 'pip')):
-                    custom_venv_choices.append(os.path.join(venv_path, d))
-
-    return custom_venv_choices
-
-
-def get_custom_venv_pip_freeze(venv_path):
-    pip_path = os.path.join(venv_path, 'bin', 'pip')
-
-    try:
-        freeze_data = subprocess.run([pip_path, "freeze"], capture_output=True)
-        pip_data = (freeze_data.stdout).decode('UTF-8')
-        return pip_data
-    except Exception:
-        logger.exception("Encountered an error while trying to run 'pip freeze' for custom virtual environments:")
-
-
 def is_ansible_variable(key):
     return key.startswith('ansible_')
 
diff --git a/awx/settings/defaults.py b/awx/settings/defaults.py
index 9120a707..2bb51ca3 100644
--- a/awx/settings/defaults.py
+++ b/awx/settings/defaults.py
@@ -179,7 +179,6 @@
 # If this setting is an empty list (the default), we will only trust ourself
 CSRF_TRUSTED_ORIGINS = []
 
-CUSTOM_VENV_PATHS = []
 
 # Warning: this is a placeholder for a database setting
 # This should not be set via a file.
diff --git a/awx/settings/development.py b/awx/settings/development.py
index df6676c6..9240a061 100644
--- a/awx/settings/development.py
+++ b/awx/settings/development.py
@@ -59,10 +59,6 @@
 SYSTEM_UUID = '00000000-0000-0000-0000-000000000000'
 INSTALL_UUID = '00000000-0000-0000-0000-000000000000'
 
-# Ansible base virtualenv paths and enablement
-# only used for deprecated fields and management commands for them
-BASE_VENV_PATH = os.path.realpath("/var/lib/awx/venv")
-
 CLUSTER_HOST_ID = socket.gethostname()
 
 AWX_CALLBACK_PROFILE = True
diff --git a/awx/settings/production.py b/awx/settings/production.py
index fa74bc7f..e6b1c8c6 100644
--- a/awx/settings/production.py
+++ b/awx/settings/production.py
@@ -30,10 +30,6 @@
 # See https://docs.djangoproject.com/en/dev/ref/settings/#allowed-hosts
 ALLOWED_HOSTS = []
 
-# Ansible base virtualenv paths and enablement
-# only used for deprecated fields and management commands for them
-BASE_VENV_PATH = os.path.realpath("/var/lib/awx/venv")
-
 # Very important that this is editable (not read_only) in the API
 AWX_ISOLATION_SHOW_PATHS = [
     '/etc/pki/ca-trust:/etc/pki/ca-trust:O',
diff --git a/awx/ui/src/screens/Setting/MiscSystem/MiscSystemDetail/MiscSystemDetail.test.js b/awx/ui/src/screens/Setting/MiscSystem/MiscSystemDetail/MiscSystemDetail.test.js
index b456090a..4cd0473f 100644
--- a/awx/ui/src/screens/Setting/MiscSystem/MiscSystemDetail/MiscSystemDetail.test.js
+++ b/awx/ui/src/screens/Setting/MiscSystem/MiscSystemDetail/MiscSystemDetail.test.js
@@ -33,7 +33,6 @@ describe('<MiscSystemDetail />', () => {
         LICENSE: null,
         INSTALL_UUID: 'db39b9ec-0c6e-4554-987d-42aw9c732ed8',
         DEFAULT_EXECUTION_ENVIRONMENT: 1,
-        CUSTOM_VENV_PATHS: [],
         AUTOMATION_ANALYTICS_LAST_ENTRIES:
           '{"foo": "2021-11-24R06:35:15.179Z"}',
       },
diff --git a/awx/ui/src/screens/Setting/shared/data.allSettingOptions.json b/awx/ui/src/screens/Setting/shared/data.allSettingOptions.json
index b9f6ef7d..da27dfb3 100644
--- a/awx/ui/src/screens/Setting/shared/data.allSettingOptions.json
+++ b/awx/ui/src/screens/Setting/shared/data.allSettingOptions.json
@@ -101,20 +101,6 @@
               "category_slug": "system",
               "default": null
           },
-          "CUSTOM_VENV_PATHS": {
-              "type": "list",
-              "required": false,
-              "label": "Custom virtual environment paths",
-              "help_text": "Paths where Tower will look for custom virtual environments (in addition to /var/lib/awx/venv/). Enter one path per line.",
-              "category": "System",
-              "category_slug": "system",
-              "default": [],
-              "child": {
-                  "type": "string",
-                  "required": true,
-                  "read_only": false
-              }
-          },
           "AD_HOC_COMMANDS": {
               "type": "list",
               "required": false,
@@ -4483,17 +4469,6 @@
               "category_slug": "system",
               "defined_in_file": false
           },
-          "CUSTOM_VENV_PATHS": {
-              "type": "list",
-              "label": "Custom virtual environment paths",
-              "help_text": "Paths where Tower will look for custom virtual environments (in addition to /var/lib/awx/venv/). Enter one path per line.",
-              "category": "System",
-              "category_slug": "system",
-              "defined_in_file": false,
-              "child": {
-                  "type": "string"
-              }
-          },
           "AD_HOC_COMMANDS": {
               "type": "list",
               "label": "Ansible Modules Allowed for Ad Hoc Jobs",
diff --git a/awx/ui/src/screens/Setting/shared/data.allSettings.json b/awx/ui/src/screens/Setting/shared/data.allSettings.json
index 4c7bc00a..f5405e05 100644
--- a/awx/ui/src/screens/Setting/shared/data.allSettings.json
+++ b/awx/ui/src/screens/Setting/shared/data.allSettings.json
@@ -15,7 +15,6 @@
   "DEFAULT_CONTROL_PLANE_QUEUE_NAME": "controlplane",
   "DEFAULT_EXECUTION_QUEUE_NAME": "default",
   "DEFAULT_EXECUTION_ENVIRONMENT": null,
-  "CUSTOM_VENV_PATHS": [],
   "AD_HOC_COMMANDS": [
       "command"
   ],
diff --git a/awx_collection/plugins/modules/inventory_source.py b/awx_collection/plugins/modules/inventory_source.py
index 5f6c1781..39713cb4 100644
--- a/awx_collection/plugins/modules/inventory_source.py
+++ b/awx_collection/plugins/modules/inventory_source.py
@@ -76,12 +76,7 @@
       description:
         - Execution Environment name, ID, or named URL to use for the source.
       type: str
-    custom_virtualenv:
-      description:
-        - Local absolute file path containing a custom Python virtualenv to use.
-        - Only compatible with older versions of AWX/Controller
-        - Deprecated, will be removed in the future
-      type: str
+
     overwrite:
       description:
         - Delete child groups and hosts not found in source.
@@ -179,7 +174,7 @@ def main():
         limit=dict(),
         credential=dict(),
         execution_environment=dict(),
-        custom_virtualenv=dict(),
+
         organization=dict(),
         overwrite=dict(type='bool'),
         overwrite_vars=dict(type='bool'),
@@ -276,7 +271,7 @@ def main():
         'source_vars',
         'overwrite',
         'overwrite_vars',
-        'custom_virtualenv',
+
         'timeout',
         'verbosity',
         'update_on_launch',
diff --git a/awx_collection/plugins/modules/job_template.py b/awx_collection/plugins/modules/job_template.py
index 81fe4951..fccb66b4 100644
--- a/awx_collection/plugins/modules/job_template.py
+++ b/awx_collection/plugins/modules/job_template.py
@@ -86,12 +86,7 @@
       description:
         - Execution Environment name, ID, or named URL to use for the job template.
       type: str
-    custom_virtualenv:
-      description:
-        - Local absolute file path containing a custom Python virtualenv to use.
-        - Only compatible with older versions of AWX/Tower
-        - Deprecated, will be removed in the future
-      type: str
+
     instance_groups:
       description:
         - list of Instance Group names, IDs, or named URLs for this Organization to run on.
@@ -400,7 +395,7 @@ def main():
         vault_credential=dict(),
         credentials=dict(type='list', elements='str'),
         execution_environment=dict(),
-        custom_virtualenv=dict(),
+
         instance_groups=dict(type="list", elements='str'),
         forks=dict(type='int'),
         limit=dict(),
@@ -538,7 +533,7 @@ def main():
         'become_enabled',
         'diff_mode',
         'allow_simultaneous',
-        'custom_virtualenv',
+
         'job_slice_count',
         'webhook_service',
         'prevent_instance_group_fallback',
diff --git a/awx_collection/plugins/modules/organization.py b/awx_collection/plugins/modules/organization.py
index 6fc406b9..3d4c343f 100644
--- a/awx_collection/plugins/modules/organization.py
+++ b/awx_collection/plugins/modules/organization.py
@@ -38,12 +38,7 @@
       description:
         - Default Execution Environment name, ID, or named URL to use for jobs owned by the Organization.
       type: str
-    custom_virtualenv:
-      description:
-        - Local absolute file path containing a custom Python virtualenv to use.
-        - Only compatible with older versions of AWX/Tower
-        - Deprecated, will be removed in the future
-      type: str
+
     max_hosts:
       description:
         - The max hosts allowed in this organizations
@@ -122,7 +117,7 @@ def main():
         new_name=dict(),
         description=dict(),
         default_environment=dict(),
-        custom_virtualenv=dict(),
+
         max_hosts=dict(type='int'),
         instance_groups=dict(type="list", elements='str'),
         notification_templates_started=dict(type="list", elements='str'),
@@ -141,7 +136,7 @@ def main():
     new_name = module.params.get("new_name")
     description = module.params.get('description')
     default_ee = module.params.get('default_environment')
-    custom_virtualenv = module.params.get('custom_virtualenv')
+
     max_hosts = module.params.get('max_hosts')
     state = module.params.get('state')
 
@@ -198,8 +193,7 @@ def main():
         org_fields['description'] = description
     if default_ee is not None:
         org_fields['default_environment'] = module.resolve_name_to_id('execution_environments', default_ee)
-    if custom_virtualenv is not None:
-        org_fields['custom_virtualenv'] = custom_virtualenv
+
     if max_hosts is not None:
         org_fields['max_hosts'] = max_hosts
 
diff --git a/awx_collection/plugins/modules/project.py b/awx_collection/plugins/modules/project.py
index 2621e57a..60c0129f 100644
--- a/awx_collection/plugins/modules/project.py
+++ b/awx_collection/plugins/modules/project.py
@@ -108,12 +108,7 @@
       description:
         - Default Execution Environment name, ID, or named URL to use for jobs relating to the project.
       type: str
-    custom_virtualenv:
-      description:
-        - Local absolute file path containing a custom Python virtualenv to use.
-        - Only compatible with older versions of AWX/Tower
-        - Deprecated, will be removed in the future
-      type: str
+
     organization:
       description:
         - Name, ID, or named URL of organization for the project.
@@ -267,7 +262,7 @@ def main():
         allow_override=dict(type='bool', aliases=['scm_allow_override']),
         timeout=dict(type='int', aliases=['job_timeout']),
         default_environment=dict(),
-        custom_virtualenv=dict(),
+
         organization=dict(),
         notification_templates_started=dict(type="list", elements='str'),
         notification_templates_success=dict(type="list", elements='str'),
@@ -369,7 +364,7 @@ def main():
         'scm_update_cache_timeout',
         'timeout',
         'scm_update_cache_timeout',
-        'custom_virtualenv',
+
         'description',
         'allow_override',
     ):
diff --git a/docs/execution_environments.md b/docs/execution_environments.md
index 8056b953..c6dfa71d 100644
--- a/docs/execution_environments.md
+++ b/docs/execution_environments.md
@@ -47,18 +47,3 @@ Jobs will use the first available execution environment in this list:
 7. Any other global EE
 
 If more than one EE fits a criteria (applies for 6 and 7), then the most recently created one will be used.
-
-## Migrating from Custom Virtual Environments
-
-If you have installed dependencies inside of custom virtual environments in
-a prior release, then have a look at this series of commands for help migrating
-dependencies out of the venvs and into EEs.
-
- - `awx-manage list_custom_venvs`
- - `awx-manage custom_venv_associations`
- - `awx-manage export_custom_venv`
-
-Follow those in order, and read the help text to see what arguments are necessary.
-
-Output from the `awx-manage export_custom_venv -q ..` command can
-be a starting point for writing an `ansible-builder` definition file.