A set of demo files to try out TRACS are available on Google Drive here:
https://drive.google.com/open?id=1FhI5w7vMiEOKwbkBmDDFbpabxZVVrKuz
File size: 2GB
If using a cloud server, you should download it directly to your server using a browser.
Unzip the archive and make sure it is available in a shared folder if using Docker:
- For TRACS Docker on Windows, this is your
C:\drive. - For TRACS Docker on Linux, this is whatever path you launched TRACS Docker with using
/path/to/folder, ie$HOME/ - For TRACS Docker On Mac OS, this is your
/Volumespath so you can place it in any drive.
This zip archive contains:
- the initial library CSV file (a table of all sgRNAs)
- one initial library read
- two replicates of Cas9-positive Control reads (A and B)
- two replicates of Cas9-negative Control reads (A and B)
- two replicates of Cas9-positive Mutant reads (A and B)
- two replicates of Cas9-negative Mutant reads (A and B)
The Control group is the Initial condition.
The Mutant group is the Final condition.