diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 6ba8f4f..4ca4be7 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -52,6 +52,22 @@ jobs:
working-directory: packages/js
- run: pytest tests/test_experiment.py
- run: git diff --exit-code -- site/js/runtime.js site/js/dodge-runtime.js site/toy-bundle.json site/license.txt
+ synaptic-reference:
+ runs-on: ubuntu-latest
+ timeout-minutes: 10
+ steps:
+ - uses: actions/checkout@v4
+ - uses: actions/setup-python@v5
+ with:
+ python-version: "3.12"
+ - run: python -m pip install -e . 'Brian2==2.9.0' 'numpy==1.26.4'
+ - name: Independent synaptic dynamics oracle
+ run: python scripts/validate_synaptic_reference.py --output synaptic-reference.json
+ - uses: actions/upload-artifact@v4
+ if: always()
+ with:
+ name: synaptic-reference
+ path: synaptic-reference.json
godot:
runs-on: ubuntu-latest
timeout-minutes: 10
diff --git a/README.md b/README.md
index 7fbbbe5..f0fe01a 100644
--- a/README.md
+++ b/README.md
@@ -1,9 +1,15 @@
# flybrain-sdk
+> Development branch: [experimental CUDA backend](docs/cuda.md). [Full FlyWire benchmark](docs/validation/flywire-full-a16.md): 139,255 neurons, all 16.85M source rows, CPU/CUDA parity and 10 seconds of continuous simulation on A16-8Q. CUDA median 11.55 seconds per simulated second (not real time). [Train an external reflex readout](examples/REFLEX_TRAINING.md). Released v0.4.0a4 is CPU-only.
+
+
**No CUDA required.** A Python SDK for connecting small connectome simulations to games and experiments, starting with a working NumPy CPU backend.
-**Full-brain CUDA benchmark (experimental branch):** [139,255 FlyWire neurons on a real NVIDIA GPU](https://freeman-1984-coder.github.io/flybrain-sdk/fullbrain.html). Complete published proofread graph, CPU/CUDA checks, reproducible timing and evidence. Released v0.4.0a4 remains CPU-only.
+**Full-brain sensory result:** [The GPU odor probe activates the input cells but exposes a propagation limit in the benchmark weight preset](docs/validation/flywire-odor-a16.md). Numerical parity does not establish biological behavior. [Published report](https://freeman-1984-coder.github.io/flybrain-sdk/fullbrain.html#olfaction).
+
+**Full-brain voxel recording:** [Watch the complete GPU brain drive a ground body](https://freeman-1984-coder.github.io/flybrain-sdk/foraging.html). The fixed readout curves away from food; the input-silenced control stays still. [Evidence, assumptions and reproduction](docs/validation/flywire-voxel-a16.md).
+**Synaptic dynamics verified on GPU:** [The separate mV engine matches Brian2 and passes actual CUDA tests](docs/synaptic-dynamics.md). [A complete FlyWire sensory probe now reaches downstream populations](docs/validation/flywire-synaptic-odor-a16.md); the candidate DNa02 readout does not simply encode stimulus side. Navigation remains unproven.
**Live game:** [Open the browser sandbox](https://freeman-1984-coder.github.io/flybrain-sdk/live.html) — add obstacles, tune mappings, inspect neural control, save and verify recordings. [Adapter guide](docs/live-sandbox.md).
@@ -23,7 +29,7 @@ brain.step(100)
print(brain.action().to_dict())
```
-**0.4 alpha:** the bundled offline demo is a hand-authored 12-neuron circuit. A separate 3.8 MB MaleCNS model now runs 313 real source neurons and 20,607 anatomical edges with explicitly assumed LIF parameters. [Model card and reproducible recipe](models/male-cns-escape-v1/README.md). CUDA and WASM are reserved interfaces, not implemented runtimes. No GPU, credentials, or network access are needed to run the toy demo after installation.
+**0.4 alpha:** the bundled offline demo is a hand-authored 12-neuron circuit. A separate 3.8 MB MaleCNS model now runs 313 real source neurons and 20,607 anatomical edges with explicitly assumed LIF parameters. [Model card and reproducible recipe](models/male-cns-escape-v1/README.md). This development branch adds an experimental CUDA runtime; WASM remains unimplemented. No GPU, credentials, or network access are needed to run the toy demo after installation.
## Make your own demo
@@ -105,7 +111,7 @@ Game movement and rendering stay in your application; see [game_loop.py](example
| --- | --- |
| `cpu` | Working reference implementation, NumPy edge lists, float64 |
| `wasm` | Reserved name; raises `BackendUnavailableError` |
-| `cuda` | Reserved name; raises `BackendUnavailableError`; no CUDA dependencies |
+| `cuda` | Experimental optional CuPy runtime; 12/12 A16 hardware cases passed; unavailable without NVIDIA/CuPy |
See [dynamics and backend contract](docs/architecture.md) for equations, spike timing and limitations.
diff --git a/docs/api.md b/docs/api.md
index f9a8864..d235091 100644
--- a/docs/api.md
+++ b/docs/api.md
@@ -138,7 +138,9 @@ Use `flybrain.experiment.replay_experiment(recording, download=False)` to verify
accepts an observation with `offer(seq, observation)`, then commits the actual
engine control with `acknowledge(seq, applied)`. Only one action may be pending.
Identical pending offers reuse their result without reintegrating the brain.
-`snapshot()` is allowed at acknowledged boundaries; `from_snapshot()` restores
-the built-in linear encoder and rate readout. The engine must checkpoint its own
+`snapshot()` is allowed at acknowledged boundaries; `from_snapshot(data, backend=None)` restores
+the built-in linear encoder and rate readout. In this CUDA development branch,
+pass `backend="cpu"` or `backend="cuda"` to select the restore device explicitly.
+The engine must checkpoint its own
world at the matching sequence. See the [Godot example](../examples/godot/README.md)
for transport, numeric validation, timing limits and recovery rules.
diff --git a/docs/cuda.md b/docs/cuda.md
new file mode 100644
index 0000000..3cb6cd0
--- /dev/null
+++ b/docs/cuda.md
@@ -0,0 +1,95 @@
+# Experimental CUDA backend — A16 hardware validation passed
+
+This development branch implements a CuPy/CUDA reference backend. **All 12 required hardware cases passed on a Vultr NVIDIA A16-2Q on 2026-09-12 UTC.** The published v0.4.0a4 remains CPU-only. This is experimental compatibility support, not a speedup claim: the 313-cell model took 2.64 seconds per simulated second on this GPU, versus 0.108 seconds on the same host CPU. See [the measured report](validation/a16-20260912.md).
+
+## Optional installation
+
+The basic `pip install -e .` path still installs only NumPy. Importing `flybrain` or creating a CPU brain does not import CuPy or initialize CUDA. On an NVIDIA host with a suitable driver, use a separate Python 3.12 environment:
+
+```sh
+pip install -e '.[dev]' 'cupy-cuda12x[ctk]==14.2.0'
+python scripts/validate_cuda.py --output cuda-validation.json
+```
+
+CuPy 14 requires Python 3.10+; the CPU SDK still supports Python 3.9. Match the CuPy wheel to the host driver/toolkit and install only one CuPy distribution. The `ctk` extra installs compatible CUDA components; it cannot supply the host NVIDIA driver. See [CuPy installation](https://docs.cupy.dev/en/stable/install.html).
+
+After hardware validation:
+
+```python
+from flybrain import FlyBrain, available_backends
+
+print(available_backends()) # dependency/device probe, not a conformance certificate
+brain = FlyBrain.load("male-cns-escape-v1", backend="cuda", download=True)
+brain.stimulate("looming_left", duration_ms=100)
+brain.advance(duration_ms=100)
+print(brain.action().to_dict())
+brain.save("gpu.checkpoint.json")
+cpu = FlyBrain.restore("gpu.checkpoint.json", backend="cpu")
+```
+
+Unavailable dependencies, devices or kernel compilation raise `BackendUnavailableError`. There is no silent CPU fallback. Python-only `backend="cuda"` does not add CUDA to the browser runtime; WASM is still unimplemented.
+
+## What the implementation does
+
+- One CUDA thread owns one postsynaptic row. Stable ordering keeps original parallel-edge accumulation order. It uses float64 and disables fused multiply-add to preserve separate reference operations; the reported hardware suite measures conformance.
+- The graph, voltage, spikes, refractory counters, filtered rates and silencing mask reside on one GPU. Each instance owns a stream so external CuPy stream contexts do not reorder its operations.
+- One kernel advances one tick using previous-tick spikes. It writes separate output buffers. A scalar error flag is checked before committing the tick, preserving state on nonfinite voltage errors.
+- Selected observations transfer only requested cells/fields. Full observations and checkpoints explicitly transfer full state. Restore uses the existing CPU validation rules, with temporary CPU graph/state allocation.
+- Current vectors are currently prepared on the host and uploaded every tick. Each tick also synchronizes an error flag. This first implementation prioritizes compatibility; it is **not** the future device-resident batched schedule and may be slower than CPU on small models.
+
+The [CuPy RawKernel API](https://docs.cupy.dev/en/stable/reference/generated/cupy.RawKernel.html) provides runtime compilation. Larger graphs, batching, mixed precision and trainable dynamics need separate work and evidence.
+
+## The hardware gate
+
+`validate_cuda.py` requires an actual CuPy-visible NVIDIA device, runs the hardware suite with `FLYBRAIN_REQUIRE_CUDA=1`, rejects skipped tests, then times toy and real circuits on CPU and GPU. Without a device it writes a failed report and exits nonzero. Ordinary CPU CI skips those hardware tests explicitly.
+
+The suite has 12 required cases. It compares every cell over 180 ticks for toy, real and signed/parallel-edge synthetic graphs at two timesteps (1,080 comparison ticks), plus cross-device checkpoints with pending stimuli, custom readout, interventions, selected observations, invalid-state handling and stream isolation. Spikes/clock must match exactly; voltage/rates use absolute tolerance `1e-10`. A report includes environment versions, device, source hashes, passed test counts, configuration, warmup and per-run timings. This covers the reported hardware and cases only.
+
+Acceptance before merging/advertising CUDA:
+
+1. No skipped or failed hardware tests on at least one NVIDIA GPU.
+2. Checkpoint continuation and direct/named current semantics match CPU.
+3. Honest CPU/GPU wall times with compilation/loading separated and synchronization included.
+4. Store the report with the exact tested source identity and inspect any failures.
+5. Re-run normal CPU packaging/CI; missing CUDA never breaks CPU installation.
+
+## Optional rented T4 run
+
+A prepared runner is available at `scripts/validate_cuda_modal.py`. Its configuration is one T4, one CPU core, 4 GiB RAM, at most one container, a 600-second function timeout, no retries, and scale-to-zero. There is no persistent volume, web endpoint or schedule. Only source code, the hardware test, validator and the small model are uploaded; no credentials or local home directory are included.
+
+After the account and spending authorization are resolved, a maintainer can run:
+
+```sh
+# Separate Python 3.10+ environment on the controlling computer.
+pip install 'modal==1.5.5'
+modal setup
+modal run scripts/validate_cuda_modal.py --output cuda-validation.json
+```
+
+This command starts remote compute and can incur charges. As checked on 2026-09-10, [Modal's published pricing](https://modal.com/pricing) lists T4 at $0.000164/second, CPU at $0.0000131/core/second, and RAM at $0.00000222/GiB/second. Ten minutes at the configured allocations is about $0.112 in execution charges, before build/startup/other charges. This estimate is not an enforced account-wide dollar cap. Review current pricing and available account credits before running; no credits are assumed. Use a $1 trial budget and verify the ephemeral app stops in the provider console when the run finishes or is canceled.
+
+The runner definition was checked against the local Modal SDK without invoking any remote function. It remains untested remotely. [Modal GPU documentation](https://modal.com/docs/guide/gpu) describes device selection. Recheck container termination and actual billed usage before considering the rental step finished.
+
+## Game integration (development branch only)
+
+The draft now includes the v0.4.0a4 Godot and project-generation changes.
+`make_demo(..., backend="cuda")` selects CUDA for Python sessions.
+`ExternalController.from_snapshot(data, backend="cpu")` explicitly restores a
+GPU checkpoint onto CPU, or vice versa with `backend="cuda"`. The new hardware
+cases compare both session and acknowledged external feedback against CPU for toy
+and real circuits, including CUDA → CPU → CUDA continuation. Both cases passed on the reported A16 device.
+
+On a configured NVIDIA host, the experimental Godot bridge accepts
+`python examples/godot/bridge.py --backend cuda`. Its chosen backend applies to
+fresh sessions and imported checkpoints. The default remains CPU; a saved backend
+label cannot change the bridge's explicitly selected device. Actual Godot with
+CUDA has not been verified.
+
+## Complete FlyWire brain benchmark
+
+The [full FlyWire v783 report](validation/flywire-full-a16.md) validates all 139,255
+proofread neurons and 16,847,997 source rows on A16-8Q. CPU/CUDA parity, backend
+checkpoint replay, 10 seconds of continuous simulated time, and 171 regression
+tests passed. CUDA median 11.55 seconds per simulated second versus CPU 110.96
+seconds on that host. This is faster than the SDK CPU reference, but not real time.
+[Reproduce the full-graph test](fullbrain-validation.md).
diff --git a/docs/fullbrain-validation.md b/docs/fullbrain-validation.md
new file mode 100644
index 0000000..634b275
--- /dev/null
+++ b/docs/fullbrain-validation.md
@@ -0,0 +1,71 @@
+# Full FlyWire CUDA validation
+
+**Functional limitation:** the [full-brain odor probe](validation/flywire-odor-a16.md) found that this benchmark’s normalized weights cannot drive an initially resting, externally unstimulated neuron to threshold. Use these results for numerical conformance, not sensory-to-motor behavior.
+
+This opt-in runner loads all **139,255 proofread FlyWire v783 neurons** and every
+row in the official proofread connections table. It uses the SDK's actual
+`Connectome`, `CPUBackend`, and `CUDABackend`, without replacing their execution
+paths. No neuron selection, minimum-count pruning, or synthetic graph is used.
+
+The pinned source contains **16,847,997 neuron-pair/neuropil rows**, representing
+**54,492,922 synaptic contacts**. Multiple rows may connect the same pair in
+different neuropils. All endpoints match the official 139,255-neuron list.
+
+## Reproduce on an NVIDIA host
+
+Use the experimental CUDA branch. A 32 GB system-memory host gives headroom for
+this initial Python object-based importer. GPU memory requirements are measured
+by the runner; the raw file size is not the required GPU memory.
+
+```sh
+python -m venv .venv
+. .venv/bin/activate
+pip install -e '.[datasets]' 'cupy-cuda12x[ctk]==14.2.0' pytest
+mkdir -p data results
+curl -fL 'https://zenodo.org/records/10676866/files/proofread_root_ids_783.npy?download=1' -o data/proofread_root_ids_783.npy
+curl -fL 'https://zenodo.org/records/10676866/files/proofread_connections_783.feather?download=1' -o data/proofread_connections_783.feather
+python scripts/validate_fullbrain.py --data-dir data --output results/fullbrain.json
+```
+
+Download source: [FlyWire Consortium, v783.0, Zenodo](https://zenodo.org/records/10676866).
+The runner checks both published MD5 values, then records SHA-256 values and byte
+sizes. A missing or different file fails the test. The raw data are not bundled
+in the package or redistributed by this repository.
+
+The neuron list stores IDs as `uint64`, while the connection table uses `int64`.
+Normalize both to the same integer type before lookup: mixed signed/unsigned
+NumPy searches can promote large IDs to `float64` and lose their identity. The
+runner rejects floating-point IDs and includes regression cases above 2^53.
+
+## What is checked
+
+- All official neuron IDs are unique; all source edge endpoints resolve.
+- Every published proofread connection row is retained, including weak connections,
+ self-connections, and parallel rows for different neuropils.
+- The complete network rests without input, then generates spikes under seeded input.
+- CPU and CUDA states across every neuron are compared every 25 ticks over 200
+ active ticks: spikes and refractory counters exactly, voltage/rates within 1e-10.
+- A JSON checkpoint reproduces 30 subsequent CUDA ticks exactly.
+- Three 1,000 ms runs per backend follow a 100-tick warmup. Compilation/loading are
+ reported separately; timed steps include host input upload and error synchronization.
+- 10,000 additional CUDA ticks check finite, bounded states and record population
+ activity, device allocations, and peak host memory. These observation-heavy
+ timings are separate from the throughput benchmark.
+
+## What this means biologically
+
+The whole published proofread **brain** graph is used; this is not the whole
+nervous system or the raw EM volume, and not every unproofread segment is a neuron
+in this graph. Each source row aggregates anatomical contacts between a neuron
+pair in a neuropil. A connection row is not one anatomical synapse.
+
+Dynamics are dimensionless, simplified LIF assumptions. Contact counts are scaled
+to incoming absolute weight sum 2.5. Each neuron's sign uses its outgoing
+contact-weighted transmitter scores: GABA/glutamate negative, other labels positive.
+Unknown transmitter evidence uses positive sign and is counted. This deliberately
+simple policy does not model receptor-specific effects or neuromodulation.
+
+The benchmark uses artificial currents, not reconstructed vision, behavior,
+physiological calibration, or biological learning. Passing establishes full-graph
+software execution and numerical checks for the reported configuration. It does
+not establish that a living fly's complete brain function has been reproduced.
diff --git a/docs/olfactory-experiment.md b/docs/olfactory-experiment.md
new file mode 100644
index 0000000..a5b3f98
--- /dev/null
+++ b/docs/olfactory-experiment.md
@@ -0,0 +1,107 @@
+# Full-brain food-odor experiment
+
+**GPU probe completed; navigation not demonstrated.**
+See the [negative result and parameter diagnosis](validation/flywire-odor-a16.md). The full FlyWire graph has
+passed the [CUDA numerical benchmark](fullbrain-validation.md). This experiment
+adds anatomically identified sensory input. It does not establish that the fly
+recognizes a banana, seeks food, or flies. No CUDA is needed to build the mapping,
+use the local odor adapter or run unit tests. The full-brain probe requires CUDA
+and fails if unavailable; it cannot silently run a smaller circuit or use CPU.
+
+## What “banana odor” means here
+
+A banana is a mixture of volatile chemicals, varying with ripeness. There is no
+single banana neuron. [Banana odor experiments](https://pubmed.ncbi.nlm.nih.gov/37357260/)
+report ripeness and concentration dependent attraction. Our first stimulus is
+**one ethyl-acetate-responsive receptor channel, Or42b → ORN_DM1**. The receptor
+response has [experimental support](https://www.nature.com/articles/s41598-017-13015-w).
+This is neither a reconstructed banana blend nor the complete response to ethyl
+acetate, which can recruit other receptors too.
+
+The pinned [FlyWire annotations](https://github.com/flyconnectome/flywire_annotations/tree/8587524c1748ce5ef2080822a2fc890fc03bf597)
+resolve 35 left and 33 right ORN_DM1 cells. All 68 exact root IDs occur in the
+official 139,255-cell v783 graph. The input population is small; **the simulated
+network remains the complete graph**, including all edges used by the full-brain
+benchmark. IDs are preserved as decimal strings, avoiding rounding above 2^53.
+Unannotated sides, unknown selected IDs and source checksum changes fail closed.
+
+The mapping also identifies 685 ALPNs (antennal-lobe projection neurons), 96
+MBONs, 1,303 descending neurons and one DNa02 per side for observation. A group
+label describes anatomical identity; it does not establish that every group
+member participates in this particular odor response.
+
+## Reproduce the mapping and GPU probe
+
+Start with the CUDA development branch and the full-brain data/environment
+instructions in [fullbrain-validation.md](fullbrain-validation.md). Download the
+annotation file into your experiment folder, outside the repository:
+
+```sh
+curl --fail --location \
+ https://raw.githubusercontent.com/flyconnectome/flywire_annotations/8587524c1748ce5ef2080822a2fc890fc03bf597/supplemental_files/Supplemental_file1_neuron_annotations.tsv \
+ --output annotations.tsv
+python scripts/build_olfactory_map.py \
+ --annotations annotations.tsv \
+ --roots data/proofread_root_ids_783.npy \
+ --output results/olfactory-map.json
+python scripts/probe_fullbrain_odor.py \
+ --annotations annotations.tsv --data-dir data \
+ --output results/fullbrain-odor.json
+```
+
+Annotation SHA-256:
+`9a4f8b2f843196074431ebd7cd883536afa1be86c8a4ce90970441e8be81d1be`.
+The output includes the pinned source URL, commit, checksum, selections and all
+assumptions. The annotation repository does not specify a license in its pinned
+root; **do not assume our MIT license covers upstream annotations**. We download
+them at the user's request and do not bundle the annotation table in the SDK.
+Cite Schlegel et al. (2024), Matsliah et al. (2024), Berg et al. (2025/2026), and
+Dorkenwald et al. (2024), as requested by the source repository. The graph has
+separate source/license metadata in the full-brain data instructions.
+
+## Sensory and world assumptions
+
+`flybrain.olfaction.OlfactoryDrive` takes two local antenna concentration samples.
+It injects only the selected sensory neurons. It receives no banana coordinates,
+target bearing, desired turn or reward. Its stateless response is
+`current = 5 * concentration / (0.2 + concentration)` with dimensionless units;
+these engineering defaults are **not fitted dose-response parameters**, pA, ppm,
+or measured spike rates. A concentration of one produces current 4.1667. Other
+channels can be added explicitly by creating additional adapters and summing
+their vectors. There is intentionally no scientifically unqualified `banana`
+preset.
+
+`sample_odor(antenna_xyz, source_xyz)` is a game-side isotropic Gaussian field.
+Sample it separately at each antenna. It models neither turbulent plumes nor
+obstacle-induced airflow. The body/world owns spatial coordinates; the neural
+controller receives concentrations only. The initial world will be a kinematic
+body in a voxel scene, not a reconstruction of flight musculature or a full
+ventral nerve cord. FlyWire v783 is a whole-brain dataset, not the whole animal's
+nervous system.
+
+## Falsifiable first gate
+
+Five conditions reset to the identical resting checkpoint: no odor, left odor,
+right odor, bilateral odor, and bilateral odor with the 68 ORNs silenced. Each
+has 100 ms pre-stimulus, 500 ms stimulus, and 200 ms recovery. The graph, initial
+state and dynamics stay fixed, with no background current, noise or tuning.
+Spikes are counted every tick and rates/voltages sampled every 10 ms. Reports
+retain every condition, the hardware, graph and script hashes, including failures.
+
+Input/silencing checks are a software validation gate. The `completed` report
+status means the experiment executed, **not** that navigation was successful.
+If sensory neurons fire but ALPNs or descending neurons do not, the current LIF
+preset has not demonstrated transmission to motor output. This is a result to
+investigate, not a reason to inject current directly into motor neurons or to
+hide a seek-food rule in the renderer.
+
+The existing benchmark uses homogeneous dimensionless LIF neurons and incoming
+absolute-weight normalization. Its numerical accuracy does not validate its
+physiology. Before a biological behavior claim, compare odor propagation under
+published synaptic/membrane dynamics and calibrated sensory drive. A later
+external learned readout must be labeled separately from innate circuit behavior.
+
+For context, the [Minecraft project's own architecture report](https://github.com/blendi-remade/fly-brain-minecraft/blob/main/docs/ARCHITECTURE.md)
+states that its odor stimulus causes activity without a walking command, so a
+handwritten body reflex implements odor taxis. Its visual appeal is useful
+inspiration; the video alone is not evidence of neural food seeking.
diff --git a/docs/synaptic-dynamics.md b/docs/synaptic-dynamics.md
new file mode 100644
index 0000000..d8cf5db
--- /dev/null
+++ b/docs/synaptic-dynamics.md
@@ -0,0 +1,148 @@
+# Synaptic LIF research engine: independent CPU reference
+
+**CPU reference and actual CUDA numerics verified; full-graph sensory propagation
+observed. Odor-directed navigation is not established.** This is the next implementation step after the
+[full-brain odor probe](validation/flywire-odor-a16.md) showed that the numerical
+benchmark's incoming normalization cannot support sensory propagation.
+
+The existing dimensionless `FlyBrain` API, models and checkpoints keep their
+original meaning. The new interface lives in `flybrain.experimental.synaptic`;
+it is not yet exposed by `FlyBrain.load()` or a model catalog entry. It requires
+explicit millivolt weights and rejects dimensionless weight units. The original
+benchmark is retained as a reproducible historical numerical test.
+
+## Model and source
+
+Nominal equations and parameters follow [Shiu et al., Nature (2024)](https://www.nature.com/articles/s41586-024-07763-9)
+and the author's [pinned Brian2 implementation](https://github.com/philshiu/Drosophila_brain_model/blob/91bdd1e7dcf193f3e7ca5a8933497fcef63b7960/model.py):
+
+```
+dv/dt = (v_rest - v + g) / tau_mem
+dg/dt = -g / tau_syn
+```
+
+`v` and `g` have mV units. Here `g` is a voltage-valued synaptic drive, not a
+conductance in siemens. A source spike schedules a signed increment to `g` after
+the transmission delay. The new engine uses the exact solution of the two
+coupled linear equations between events, including the equal-time-constant limit.
+
+| Parameter | Nominal value |
+|---|---:|
+| Step | 0.1 ms |
+| Rest / reset | −52 mV |
+| Strict spike threshold | greater than −45 mV |
+| Membrane time constant | 20 ms |
+| Synaptic decay time constant | 5 ms |
+| Refractory interval | 2.2 ms |
+| Transmission delay | 1.8 ms |
+
+The paper uses **0.275 mV per anatomical contact**, with transmitter-dependent
+signs. This is a fitted model parameter, not a measurement of every synapse.
+Callers must supply already-converted edge weights. The engine never normalizes
+them by incoming degree. Receptor/neuromodulator mechanisms, membrane compartments,
+gap junctions, body mechanics, learning and model calibration are not implemented.
+Applying these nominal parameters to another graph/version is a new experiment,
+not automatic replication of the paper's biological results.
+
+## Event schedule and checkpoints
+
+One step represents the schedule at `t = previous_tick * dt`:
+
+1. Update refractory eligibility and integrate `v` and `g` exactly.
+2. Detect threshold crossings.
+3. Deliver delayed synaptic events and declared external voltage jumps.
+4. Reset spiking cells' `v` and `g`, then update recorded rates.
+
+Both states are frozen and protected from synaptic writes during refractoriness,
+matching Brian2's `(unless refractory)` semantics. Eligibility uses integer tick
+differences `>= refractory_ticks`, verified against Brian2 2.9.0. Source neurons
+listed in `input_ids` have zero refractory duration, as in the paper's Poisson
+targets. An externally injected jump can trigger a spike on the next step; an
+input coinciding with a reset can be cleared. The oracle exercises this ordering.
+Delay and refractory durations must be exact multiples of the timestep; there is
+no hidden rounding to a different biological duration.
+
+Snapshots contain `g`, membrane voltage, rates, silencing, last-spike times and
+the complete circular buffer of delayed spikes. A fingerprint binds the graph,
+weights, dynamics and declared input neurons. Cross-model restores are rejected.
+Restore validates state before replacing it. Silencing suppresses future spikes;
+events already queued before silencing retain their scheduled delivery.
+
+`step(voltage_jumps_mv)` accepts a finite vector, with nonzero entries only at
+declared inputs. This engine has no internal RNG. A later Poisson encoder must
+save its own RNG state with the experiment. The original paper uses stochastic
+inputs with jump amplitude `0.275 * 250 = 68.75 mV`; validation here uses known
+event times, separating solver correctness from random-number generator choice.
+
+## Reproduce independent verification
+
+In an isolated Python 3.12 environment:
+
+```sh
+pip install -e . 'Brian2==2.9.0' 'numpy==1.26.4'
+python scripts/validate_synaptic_reference.py --output synaptic-reference.json
+```
+
+Brian2 2.9.0 accesses an API removed in NumPy 2, so this **oracle environment**
+pins NumPy 1.26.4. The SDK's ordinary CPU installation still supports NumPy 2;
+Brian2 is not added to runtime dependencies.
+
+The oracle independently constructs the same equations in Brian2 and compares
+every cell at every step, with explicit scheduled input events. Four cases cover
+0.1/0.2 ms steps, positive/negative and parallel edges, recurrent edges, an
+autapse, zero/positive delays, simultaneous input/reset, isolated cells and equal
+time constants. Each case includes real downstream spikes in the synthetic test
+network. These small fixtures validate the solver; they are not fly models.
+
+Local result: **all four cases passed**, identical spike ticks, membrane errors
+at most approximately `5.05e-13 mV`, synaptic-state errors at most approximately
+`3.56e-14 mV`. JSON checkpoints taken while an event is in flight replay exactly.
+[Machine-readable oracle report](validation/synaptic-brian2-reference.json).
+Eleven dependency-free unit cases additionally cover delayed delivery, downstream
+activation, invalid units/input selections, incompatible or malformed checkpoints,
+silencing, and the near-equal time-constant limit. CI runs the Brian2 oracle in its own environment.
+
+For equal time constants, the oracle uses the same symbolic time constant in both
+equations. Brian2's generic expression for distinct symbolic constants divides
+by their difference when they happen to have equal numeric values. Using the
+equivalent single-symbol equations permits its independent exact solver to
+derive the degenerate case, rather than evaluating that removable singularity.
+
+## Required next gates
+
+The [actual A16 results](validation/flywire-synaptic-odor-a16.md) cover the CUDA
+schedule, full graph, active CPU/GPU comparison and matched sensory controls.
+Signals reach downstream populations, but DNa02 retains a left bias for both
+left and right stimuli. Establishing a useful motor mapping remains a separate
+experiment. Preserve negative results; target coordinates must not reach a
+hidden seek-food controller.
+## Experimental CUDA implementation and full-graph odor protocol
+
+`flybrain.experimental.synaptic_cuda.SynapticCUDA` is an optional port of this
+reference. **All five hardware cases passed on an A16-8Q**, separately from the previous
+dimensionless CUDA benchmark. See the linked report for exact scope and source. Importing its module is safe
+without CuPy; constructing the engine requires a working NVIDIA device.
+
+The actual-hardware gate must execute all five GPU cases without skips:
+
+```bash
+python scripts/validate_synaptic_cuda.py --output synaptic-cuda.json
+```
+
+After that gate passes, run the complete FlyWire odor protocol using the same
+verified source files and annotations as [the earlier experiment](olfactory-experiment.md):
+
+```bash
+python scripts/probe_synaptic_odor.py --data-dir data \
+ --annotations data/annotations.tsv --output synaptic-odor.json
+```
+
+It retains every official neuron and aggregate edge, uses explicitly signed
+0.275 mV/contact weights without incoming normalization, and stimulates only
+the 68 annotated DM1 inputs with nominal 150 Hz discrete Poisson events and
+68.75 mV input jumps. These are research assumptions, not a calibrated banana
+dose. It first checks 200 active full-graph CPU/GPU ticks and 20 ticks of JSON
+checkpoint replay, including the input RNG. Then it records all five matched
+800 ms conditions: no odor, left, right, bilateral, bilateral with ORNs silenced.
+Enabled input cells share identical random draws across conditions. Negative
+results are retained; completing the protocol does not establish navigation.
diff --git a/docs/validation/a16-20260912.json b/docs/validation/a16-20260912.json
new file mode 100644
index 0000000..265dfad
--- /dev/null
+++ b/docs/validation/a16-20260912.json
@@ -0,0 +1,141 @@
+{
+ "format": "flybrain-cuda-validation",
+ "schema_version": 1,
+ "status": "passed",
+ "started_at": "2026-09-12T15:00:22.452207+00:00",
+ "source_sha256": {
+ "scripts/validate_cuda.py": "113060288dc4865065542870ba102d085785327edcb6a6eb7a781e47a0528dea",
+ "src/flybrain/__init__.py": "de9596a06a4de0a04303795a10ae0a6d8cbbc7705a5e60ad566b4c1bfad65971",
+ "src/flybrain/__main__.py": "c125d73bf421cac3c6a8f4185ae56516db20cc91831e006c42c544c7b873dfb9",
+ "src/flybrain/backends/__init__.py": "6c94a5cc4be4916e569562041036a6e169b13b228d2bc2dffc0494cff90bd64a",
+ "src/flybrain/backends/base.py": "bb28e7aa30f3cb3b676243e6e3767242d5daa27458b5e5a96b218232aaa1de81",
+ "src/flybrain/backends/cpu.py": "20ff6d342efe088f161b800f825175b77b754e42378e7879e6ff5007da5fe11a",
+ "src/flybrain/backends/cuda.py": "da6f944b0075733d668615ec73f2822cdf740d404d06d2e8309afec1a63b9e9b",
+ "src/flybrain/brain.py": "e9ea19aea644bcbdcc686b6e79ee5767f4c620cdeb063cf69fd7a014a87e9450",
+ "src/flybrain/config.py": "a7f274ce09c28701db19b4dec154b30771890e9ac6a8527ddcc7f6d68e6d29b8",
+ "src/flybrain/data/__init__.py": "6b42fe29f9b5a6f3e309d4fd947212a6a49b92c095e9f815519360dc14924790",
+ "src/flybrain/demos.py": "ad45e3a91920affae56b74f2b3d2d1d0b8eab41d705acf3ccd4fbba8b8a5cee6",
+ "src/flybrain/drive.py": "b018bcf2c820ef58ab160204890b465c0702bfba6ca75c65f2d1b33ebb9cac24",
+ "src/flybrain/errors.py": "fcded1de58450030889fa069c4fa9f924344accc92418f8e93099639744f2b0d",
+ "src/flybrain/experiment.py": "ae423d017ad86e5fd1d2c49c6c33b73c6d2a339a7e299c25d1195cb2ac10d03c",
+ "src/flybrain/external.py": "8b853fe406607bc13c2680e3d686c0b6ca6fc470328a0dbcdce4590f72585f34",
+ "src/flybrain/importers/__init__.py": "d6171134397cf1089cc14419f114172a77863ca90c83bc8cb6780dcb3cc74394",
+ "src/flybrain/importers/malecns.py": "f0b1fda6403807f127eb839510f66cc567143535a479446e66ed8bcfdea6507d",
+ "src/flybrain/model.py": "4ee80b0dc5cc9b8d31067b7817e42b273660cdd7b2ac469eae7146a027824173",
+ "src/flybrain/motor.py": "7dac900576636fb267e5a4a9578731bf5b541e8ea5baf747476fe56db29c8026",
+ "src/flybrain/neurons.py": "9cfb9c65e7940ec3e930273a00b15417615a486a348aef362ec4760067233690",
+ "src/flybrain/projects.py": "ec85f320ac654d11da885d72825ede716b25be2d6d1d7d91795e78db8252a3f2",
+ "src/flybrain/registry.py": "503d2eb6e9124b3c2fe7452f4ca8edd1f5128d135a0a406d1c902bf01cb0ef81",
+ "src/flybrain/sensory.py": "13403eb880fc7c04496d8f5a0405d7b7c116f19b0a69887dff25368c5d291dd3",
+ "src/flybrain/session.py": "0e04b67cc0f32f4d5b109b2e8bfdb9082d0f27e295df2718b5a597f8a2e3192a",
+ "src/flybrain/viewer.py": "26d71040003ac8deff35bd5c0fb42be9d47a0af49a41754795b20fb608910cad",
+ "tests/test_cuda_hardware.py": "16ec1436ca16842edcc095a249e061ed797c94d644dcf428ee20399fca6bc102"
+ },
+ "environment": {
+ "python": "3.12.3",
+ "platform": "Linux-6.8.0-138-generic-x86_64-with-glibc2.39",
+ "numpy": "2.2.6",
+ "cupy": "14.2.0",
+ "device": "NVIDIA A16-2Q",
+ "compute_capability": [
+ 8,
+ 6
+ ],
+ "device_memory_bytes": 1964965888,
+ "cuda_driver": 12040,
+ "cuda_runtime": 12090
+ },
+ "conformance": {
+ "tests": 12,
+ "failures": 0,
+ "errors": 0,
+ "skipped": 0
+ },
+ "benchmarks": [
+ {
+ "model": "toy-v1",
+ "fingerprint": "ba1701cf092e8b6acb8d36aa70f86f46b4521e4bb5f6f463efb3d8824b4bac6c",
+ "neurons": 12,
+ "edges": 12,
+ "config": {
+ "dt_ms": 1.0,
+ "tau_ms": 10.0,
+ "rest": 0.0,
+ "reset": 0.0,
+ "threshold": 1.0,
+ "refractory_ms": 2.0,
+ "input_gain": 2.0,
+ "rate_tau_ms": 50.0,
+ "action_rate_hz": 100.0
+ },
+ "simulated_ms_per_repeat": 1000,
+ "warmup_ms": 100,
+ "timings": {
+ "cpu_load_seconds": 0.0002442290000317371,
+ "cpu": {
+ "seconds": [
+ 0.02785182699994948,
+ 0.02760493999994651,
+ 0.027172675999963758
+ ],
+ "median_seconds": 0.02760493999994651
+ },
+ "cuda_load_seconds": 0.12077638699997806,
+ "cuda": {
+ "seconds": [
+ 2.557725829999981,
+ 2.629756971000006,
+ 2.527393785999948
+ ],
+ "median_seconds": 2.557725829999981
+ }
+ }
+ },
+ {
+ "model": "male-cns-escape-v1",
+ "fingerprint": "47a0c91b3eba9c606c29faef58fe15c8846fc049c4314a5e3b6912ab22c5e68a",
+ "neurons": 313,
+ "edges": 20607,
+ "config": {
+ "dt_ms": 1.0,
+ "tau_ms": 10.0,
+ "rest": 0.0,
+ "reset": 0.0,
+ "threshold": 1.0,
+ "refractory_ms": 2.0,
+ "input_gain": 2.0,
+ "rate_tau_ms": 50.0,
+ "action_rate_hz": 100.0
+ },
+ "simulated_ms_per_repeat": 1000,
+ "warmup_ms": 100,
+ "timings": {
+ "cpu_load_seconds": 0.1308009670000274,
+ "cpu": {
+ "seconds": [
+ 0.11087149200000113,
+ 0.10837631200001852,
+ 0.10773501699998178
+ ],
+ "median_seconds": 0.10837631200001852
+ },
+ "cuda_load_seconds": 0.11967941799991877,
+ "cuda": {
+ "seconds": [
+ 2.63953883399995,
+ 2.582605983999997,
+ 2.640352118999999
+ ],
+ "median_seconds": 2.63953883399995
+ }
+ }
+ }
+ ],
+ "limitations": [
+ "Correctness-first CUDA: host current upload and scalar synchronization each tick.",
+ "These timings cover only toy and 313-cell circuit, not whole-brain scaling.",
+ "Loading/compilation reported separately; execution includes inputs and action readout.",
+ "Conformance applies to tested cases and reported device/software, not all CUDA hardware."
+ ],
+ "finished_at": "2026-09-12T15:01:02.554374+00:00"
+}
diff --git a/docs/validation/a16-20260912.md b/docs/validation/a16-20260912.md
new file mode 100644
index 0000000..af9814b
--- /dev/null
+++ b/docs/validation/a16-20260912.md
@@ -0,0 +1,50 @@
+# Actual NVIDIA validation — 12 September 2026 UTC
+
+Source commit: `35628f379d79b5ceefcec8fb0acc9bf04baa610b`.
+[Machine-readable conformance report](a16-20260912.json) includes SHA-256 for
+every Python runtime source, hardware test, and validator. Those hashes were
+checked against the local source before accepting the report.
+
+- Vultr Frankfurt, NVIDIA A16-2Q vGPU, advertised 2 GB framebuffer.
+- Python 3.12.3, NumPy 2.2.6, CuPy 14.2.0.
+- NVIDIA driver 550.90.07, reported driver API 12.4, runtime API 12.9.
+- Hardware gate: **12 passed, zero skipped**, 22.43 seconds.
+- Full Python suite on this host: **161 passed, 1 optional import skipped**,
+ 48.83 seconds. PyArrow was not installed; the separate GitHub quality job tests
+ the Arrow importer with that dependency installed.
+- GitHub CI on this commit passed all seven jobs, including Python across
+ Linux/macOS/Windows, packaging, JavaScript parity, and actual Godot checks.
+
+## Measured small-circuit latency
+
+Three repeats, median wall seconds per 1,000 ms simulated time, after 100 ms
+warmup. Execution includes input handling, readout and GPU synchronization.
+Loading/compilation is separate in the JSON.
+
+| Model | CPU | CUDA |
+|---|---:|---:|
+| Artificial toy, 12 cells | 0.02760 s | 2.55773 s |
+| MaleCNS escape, 313 cells | 0.10838 s | 2.63954 s |
+
+**CUDA is slower on these small circuits.** This reference path uploads currents
+and synchronizes an error flag on each tick. A fractional virtual GPU also has
+scheduling overhead. These data do not establish whole-brain scaling, speedup,
+or full-card A16 performance. Keep CPU as the default. Device-resident multi-tick
+schedules and batching are the next optimization target, with the same
+conformance gate retained.
+
+The first device attempt failed at NVRTC compilation because `math.h` was not
+provided by the pip CUDA environment. Removing that unnecessary host header
+allowed NVRTC's built-in device math to compile. The complete gate then passed;
+CPU-only tests could not detect this deployment issue.
+
+## External readout learning
+
+`examples/train_reflex.py --backend cuda` ran a reward-trained two-choice action
+readout using the unchanged 313-neuron circuit. On 80 balanced held-out trials,
+accuracy rose from **50% to 100%** after 24 batches over 64 training trials.
+Runtime was 33.87 seconds. CPU independently reproduced the same accuracy.
+
+Only three external readout weights were trained. This is a simple, resetting
+left/right obstacle task with varied stimulus strength and distractors, not
+biological plasticity or broad game skill. See the [experiment guide](../../examples/REFLEX_TRAINING.md).
diff --git a/docs/validation/benchmark-voltage-bound.json b/docs/validation/benchmark-voltage-bound.json
new file mode 100644
index 0000000..4a7dc63
--- /dev/null
+++ b/docs/validation/benchmark-voltage-bound.json
@@ -0,0 +1,21 @@
+{
+ "config": {
+ "dt_ms": 1.0,
+ "tau_ms": 10.0,
+ "rest": 0.0,
+ "reset": 0.0,
+ "threshold": 1.0,
+ "refractory_ms": 2.0,
+ "input_gain": 2.0,
+ "rate_tau_ms": 50.0,
+ "action_rate_hz": 100.0
+ },
+ "incoming_weight": 2.5,
+ "presynaptic_current": 100.0,
+ "postsynaptic_current": 0.0,
+ "ticks": 2000,
+ "max_voltage": 0.9179135027773137,
+ "postsynaptic_spikes": 0,
+ "analytic_bound": 0.9179135027773139,
+ "scope": "CPU two-cell diagnostic for the benchmark weight policy, not a full-brain run"
+}
diff --git a/docs/validation/flywire-full-a16.json b/docs/validation/flywire-full-a16.json
new file mode 100644
index 0000000..b289898
--- /dev/null
+++ b/docs/validation/flywire-full-a16.json
@@ -0,0 +1,849 @@
+{
+ "status": "passed",
+ "started_utc": "2026-09-12T16:39:35Z",
+ "python": "3.12.3",
+ "numpy": "2.5.3",
+ "script_sha256": "12bfd2df4a80be66cc75183a75f06eebcb1f46b9a160ed82472f004800cd5d41",
+ "config": {
+ "dt_ms": 1.0,
+ "tau_ms": 10.0,
+ "rest": 0.0,
+ "reset": 0.0,
+ "threshold": 1.0,
+ "refractory_ms": 2.0,
+ "input_gain": 2.0,
+ "rate_tau_ms": 50.0,
+ "action_rate_hz": 100.0
+ },
+ "model": {
+ "dataset": "FlyWire v783 / Zenodo 10676866",
+ "source_url": "https://zenodo.org/records/10676866",
+ "sources": {
+ "proofread_root_ids_783.npy": {
+ "md5": "e0e6c19732fd8c7a4e39a2d170105421",
+ "sha256": "7c7b7e818e9232e5ab64793d52ba20574dbe9da3a6509fbc74193b0f259a01be",
+ "bytes": 1114168
+ },
+ "proofread_connections_783.feather": {
+ "md5": "f48f972d262323a102aed49af1396b8a",
+ "sha256": "24f960ae3e7d4f8cd30db3b62e99fb5179cc3d1e76d8c155bfb441e9737d3faf",
+ "bytes": 852022274
+ }
+ },
+ "neurons": 139255,
+ "source_rows": 16847997,
+ "retained_rows": 16847997,
+ "discarded_rows": 0,
+ "synaptic_contacts": 54492922,
+ "rows_are": "neuron-pair/neuropil aggregates, retained as parallel weighted edges",
+ "isolated_neurons": 616,
+ "nt_score_nonfinite_entries": 10392,
+ "neurons_without_nt_evidence": 1250,
+ "assumed_sign_counts": {
+ "positive": 95386,
+ "negative": 43869
+ },
+ "weight_policy": "incoming abs-sum 2.5; GABA/GLUT negative, others/unknown positive",
+ "minimum_contacts": 1,
+ "additional_pruning": false,
+ "graph_arrays_sha256": "bd02d6a2c8a5a4a27cf482fbb333648babe7aa1747952674a25694be6cc41f5c"
+ },
+ "model_load_seconds": 63.94955811400007,
+ "cpu_init_seconds": 3.273527094999963,
+ "cuda_init_and_compile_seconds": 6.7739249129999735,
+ "device": {
+ "name": "NVIDIA A16-8Q",
+ "memory_bytes": 8394047488,
+ "cupy": "14.2.0",
+ "driver": 12040,
+ "runtime": 12090
+ },
+ "cuda_kernel_sha256": "da6f944b0075733d668615ec73f2822cdf740d404d06d2e8309afec1a63b9e9b",
+ "rest_check": "20 ticks; all neurons at rest, no spikes",
+ "parity": {
+ "ticks": 200,
+ "all_neurons_compared_every": 25,
+ "exact_spikes_and_refractory": true,
+ "atol": 1e-10,
+ "total_spikes": 1863638,
+ "checks": [
+ {
+ "tick": 45,
+ "voltage": 0.0,
+ "rates_hz": 0.0
+ },
+ {
+ "tick": 70,
+ "voltage": 0.0,
+ "rates_hz": 0.0
+ },
+ {
+ "tick": 95,
+ "voltage": 0.0,
+ "rates_hz": 0.0
+ },
+ {
+ "tick": 120,
+ "voltage": 0.0,
+ "rates_hz": 0.0
+ },
+ {
+ "tick": 145,
+ "voltage": 0.0,
+ "rates_hz": 0.0
+ },
+ {
+ "tick": 170,
+ "voltage": 0.0,
+ "rates_hz": 0.0
+ },
+ {
+ "tick": 195,
+ "voltage": 0.0,
+ "rates_hz": 0.0
+ },
+ {
+ "tick": 220,
+ "voltage": 0.0,
+ "rates_hz": 0.0
+ }
+ ]
+ },
+ "checkpoint_replay_exact": true,
+ "timings": {
+ "cpu": {
+ "wall_seconds": [
+ 110.95522328200002,
+ 110.81536086300014,
+ 113.70247338600007
+ ],
+ "median_seconds": 110.95522328200002,
+ "simulated_ms_per_repeat": 1000,
+ "warmup_ticks": 100,
+ "includes": "SDK step, host input upload and per-tick error sync"
+ },
+ "cuda": {
+ "wall_seconds": [
+ 11.551658382000142,
+ 11.55760231399995,
+ 11.549266215999978
+ ],
+ "median_seconds": 11.551658382000142,
+ "simulated_ms_per_repeat": 1000,
+ "warmup_ticks": 100,
+ "includes": "SDK step, host input upload and per-tick error sync"
+ }
+ },
+ "stability": {
+ "ticks": 10000,
+ "simulated_ms": 10000,
+ "wall_seconds_including_observation": 122.0339100839999,
+ "total_spikes": 93609984,
+ "ever_spiking_neurons": 139254,
+ "trace": [
+ {
+ "tick": 3450,
+ "voltage_min": -0.11559652259505011,
+ "voltage_max": 0.9999895975010603,
+ "mean_rate_hz": 67.06434093072953,
+ "spiking_neurons": 9954
+ },
+ {
+ "tick": 3550,
+ "voltage_min": -0.08183970444976454,
+ "voltage_max": 0.9999855442040886,
+ "mean_rate_hz": 67.31109008337248,
+ "spiking_neurons": 10455
+ },
+ {
+ "tick": 3650,
+ "voltage_min": -0.06286764324206404,
+ "voltage_max": 0.9999805083223539,
+ "mean_rate_hz": 67.2512843650405,
+ "spiking_neurons": 9951
+ },
+ {
+ "tick": 3750,
+ "voltage_min": -0.11745189490049097,
+ "voltage_max": 0.9999952816834452,
+ "mean_rate_hz": 67.35758989498028,
+ "spiking_neurons": 10186
+ },
+ {
+ "tick": 3850,
+ "voltage_min": -0.04616095464256996,
+ "voltage_max": 0.9999942284270661,
+ "mean_rate_hz": 67.22809650682696,
+ "spiking_neurons": 8925
+ },
+ {
+ "tick": 3950,
+ "voltage_min": -0.007009385116817967,
+ "voltage_max": 0.9999986690501007,
+ "mean_rate_hz": 67.19958574064901,
+ "spiking_neurons": 9730
+ },
+ {
+ "tick": 4050,
+ "voltage_min": -0.044506418753874774,
+ "voltage_max": 0.9999951806205629,
+ "mean_rate_hz": 67.34036686161755,
+ "spiking_neurons": 10501
+ },
+ {
+ "tick": 4150,
+ "voltage_min": -0.04505158655941583,
+ "voltage_max": 0.9999816411409089,
+ "mean_rate_hz": 67.18654480380874,
+ "spiking_neurons": 10014
+ },
+ {
+ "tick": 4250,
+ "voltage_min": -0.07875496389674917,
+ "voltage_max": 0.9999980287483852,
+ "mean_rate_hz": 67.24529152473103,
+ "spiking_neurons": 9961
+ },
+ {
+ "tick": 4350,
+ "voltage_min": -0.04736940171429964,
+ "voltage_max": 0.9999966045808074,
+ "mean_rate_hz": 67.0933686400383,
+ "spiking_neurons": 8844
+ },
+ {
+ "tick": 4450,
+ "voltage_min": -0.052571104472888354,
+ "voltage_max": 0.9999984261276116,
+ "mean_rate_hz": 67.07703919699696,
+ "spiking_neurons": 9925
+ },
+ {
+ "tick": 4550,
+ "voltage_min": -0.07777007824024756,
+ "voltage_max": 0.9999890797056991,
+ "mean_rate_hz": 67.29155923037514,
+ "spiking_neurons": 10338
+ },
+ {
+ "tick": 4650,
+ "voltage_min": -0.07831540385875636,
+ "voltage_max": 0.99999375592485,
+ "mean_rate_hz": 67.26288618192817,
+ "spiking_neurons": 9989
+ },
+ {
+ "tick": 4750,
+ "voltage_min": -0.10053549289188887,
+ "voltage_max": 0.9999889628571436,
+ "mean_rate_hz": 67.37750588031031,
+ "spiking_neurons": 10181
+ },
+ {
+ "tick": 4850,
+ "voltage_min": -0.10608450356278502,
+ "voltage_max": 0.9999986090582871,
+ "mean_rate_hz": 67.21681196307753,
+ "spiking_neurons": 8840
+ },
+ {
+ "tick": 4950,
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+ "gpu_pool_used_bytes": 210532864,
+ "gpu_pool_reserved_bytes": 214013952,
+ "host_peak_rss_kib_linux": 7122432
+}
diff --git a/docs/validation/flywire-full-a16.md b/docs/validation/flywire-full-a16.md
new file mode 100644
index 0000000..4256543
--- /dev/null
+++ b/docs/validation/flywire-full-a16.md
@@ -0,0 +1,63 @@
+# Full FlyWire v783 on NVIDIA A16-8Q
+
+Actual-device run, 2026-09-12 UTC. Tested source commit:
+`5cb825507a05a50c0a0c7200e3c130d547f77663`.
+The later Windows import fix only makes Linux RSS collection optional outside Linux;
+no simulation, importer mapping, or benchmark algorithm was changed by that fix.
+
+- All 139,255 proofread neurons, including 616 isolated neurons.
+- All 16,847,997 published neuron-pair/neuropil rows retained (0 discarded).
+- 54,492,922 anatomical synaptic contacts represented by those rows.
+- Both official MD5 checks passed; SHA-256 values are in the report.
+- 12/12 actual CUDA hardware checks passed; full suite **171 passed, 0 skipped**.
+- 200 active CPU/CUDA ticks, comparing every neuron every 25 ticks: exact spikes
+ and refractory state; voltage and rate maximum difference was 0 at all checkpoints.
+- JSON backend-state roundtrip reproduced 30 subsequent CUDA ticks exactly.
+- 10,000 additional CUDA ticks (10 seconds simulated), finite/bounded full states
+ checked every 100 ticks: 93,609,984 spikes, 139,254 neurons fired at least once.
+
+| SDK backend | Three wall times for 1,000 ms simulation | Median |
+| --- | --- | --- |
+| NumPy CPU | 110.955, 110.815, 113.702 s | 110.955 s |
+| CuPy CUDA | 11.552, 11.558, 11.549 s | 11.552 s |
+
+CUDA was **9.61x faster than this SDK CPU reference on this host**, still about
+11.55 wall seconds per simulated second (not real time). Timings include per-tick
+host input uploads and scalar error synchronization. This is not a comparison
+against an optimized third-party CPU simulator. Both use float64 and dt=1 ms.
+
+Model import: 63.95 s. CPU backend initialization: 3.27 s. CUDA initialization and
+compilation: 6.77 s. Stability pass including activity collection: 122.03 s.
+Final CuPy pool in-use allocation: 210,532,864 bytes (~200.8 MiB); reserved:
+214,013,952 bytes. These are not peak total GPU memory. Host process peak RSS:
+7,122,432 KiB (~6.79 GiB). The Python object graph is costly in host memory.
+
+Environment: A16-8Q, 8 GiB VRAM; 3 vCPU / 32 GiB system RAM; Ubuntu 24.04;
+Python 3.12.3, NumPy 2.5.3, CuPy 14.2.0, NVIDIA driver 550.90.07,
+CUDA runtime API 12090. See frozen dependencies for the complete installation.
+
+## Scope and assumptions
+
+This is the complete published **proofread brain graph**, not the entire CNS,
+not raw unproofread EM fragments, and not a physiological or behavioral model.
+The original 313-cell Reflex School remains a separate experiment.
+Artificial currents drive this benchmark. No learning or body/game integration
+is claimed here. GABA/glutamate signs, other/unknown positive signs, homogeneous
+LIF parameters, and incoming normalization to 2.5 are explicit assumptions.
+1,250 neurons have no outgoing transmitter-score evidence; the policy counts them
+and assumes positive sign. No edges are dropped to hide missing annotations.
+
+The first attempt failed closed because NumPy mixed int64/uint64 lookups lost
+precision on long neuron IDs. Normalizing both source columns to uint64 fixed
+this; four regression tests protect neighboring large IDs and unknown IDs.
+
+The report's checkpoint is the backend state with the same model, not a benchmark
+of serializing the entire graph through FlyBrain.save(). The whole-brain entry
+point is currently a validation script rather than a catalog-ready game model.
+
+- [Full JSON report](flywire-full-a16.json)
+- [Full pytest XML](flywire-full-pytest.xml)
+- [44 source SHA-256 values](flywire-full-source-sha256.json)
+- [Frozen requirements](flywire-full-requirements.txt)
+- [Reproduction guide](../fullbrain-validation.md)
+- [Official dataset](https://zenodo.org/records/10676866)
diff --git a/docs/validation/flywire-full-pytest.xml b/docs/validation/flywire-full-pytest.xml
new file mode 100644
index 0000000..5b1529f
--- /dev/null
+++ b/docs/validation/flywire-full-pytest.xml
@@ -0,0 +1 @@
+
\ No newline at end of file
diff --git a/docs/validation/flywire-full-requirements.txt b/docs/validation/flywire-full-requirements.txt
new file mode 100644
index 0000000..26707dd
--- /dev/null
+++ b/docs/validation/flywire-full-requirements.txt
@@ -0,0 +1,20 @@
+cuda-pathfinder==1.8.1
+cuda-toolkit==12.9.2.0
+cupy-cuda12x==14.2.0
+# Editable install with no version control (flybrain-sdk==0.5.0.dev0)
+-e /root/fullbrain/source
+iniconfig==2.3.0
+numpy==2.5.3
+nvidia-cublas-cu12==12.9.2.10
+nvidia-cuda-nvrtc-cu12==12.9.86
+nvidia-cuda-runtime-cu12==12.9.79
+nvidia-cufft-cu12==11.4.1.4
+nvidia-curand-cu12==10.3.10.19
+nvidia-cusolver-cu12==11.7.5.82
+nvidia-cusparse-cu12==12.5.10.65
+nvidia-nvjitlink-cu12==12.9.86
+packaging==26.3
+pluggy==1.6.0
+pyarrow==25.0.1
+Pygments==2.21.0
+pytest==9.1.1
diff --git a/docs/validation/flywire-full-source-sha256.json b/docs/validation/flywire-full-source-sha256.json
new file mode 100644
index 0000000..5602a60
--- /dev/null
+++ b/docs/validation/flywire-full-source-sha256.json
@@ -0,0 +1,46 @@
+{
+ "src/flybrain/session.py": "0e04b67cc0f32f4d5b109b2e8bfdb9082d0f27e295df2718b5a597f8a2e3192a",
+ "src/flybrain/demos.py": "ad45e3a91920affae56b74f2b3d2d1d0b8eab41d705acf3ccd4fbba8b8a5cee6",
+ "src/flybrain/brain.py": "e9ea19aea644bcbdcc686b6e79ee5767f4c620cdeb063cf69fd7a014a87e9450",
+ "src/flybrain/viewer.py": "26d71040003ac8deff35bd5c0fb42be9d47a0af49a41754795b20fb608910cad",
+ "src/flybrain/errors.py": "fcded1de58450030889fa069c4fa9f924344accc92418f8e93099639744f2b0d",
+ "src/flybrain/motor.py": "7dac900576636fb267e5a4a9578731bf5b541e8ea5baf747476fe56db29c8026",
+ "src/flybrain/neurons.py": "9cfb9c65e7940ec3e930273a00b15417615a486a348aef362ec4760067233690",
+ "src/flybrain/__init__.py": "de9596a06a4de0a04303795a10ae0a6d8cbbc7705a5e60ad566b4c1bfad65971",
+ "src/flybrain/model.py": "4ee80b0dc5cc9b8d31067b7817e42b273660cdd7b2ac469eae7146a027824173",
+ "src/flybrain/__main__.py": "c125d73bf421cac3c6a8f4185ae56516db20cc91831e006c42c544c7b873dfb9",
+ "src/flybrain/registry.py": "503d2eb6e9124b3c2fe7452f4ca8edd1f5128d135a0a406d1c902bf01cb0ef81",
+ "src/flybrain/drive.py": "b018bcf2c820ef58ab160204890b465c0702bfba6ca75c65f2d1b33ebb9cac24",
+ "src/flybrain/projects.py": "ec85f320ac654d11da885d72825ede716b25be2d6d1d7d91795e78db8252a3f2",
+ "src/flybrain/experiment.py": "ae423d017ad86e5fd1d2c49c6c33b73c6d2a339a7e299c25d1195cb2ac10d03c",
+ "src/flybrain/sensory.py": "13403eb880fc7c04496d8f5a0405d7b7c116f19b0a69887dff25368c5d291dd3",
+ "src/flybrain/external.py": "8b853fe406607bc13c2680e3d686c0b6ca6fc470328a0dbcdce4590f72585f34",
+ "src/flybrain/config.py": "a7f274ce09c28701db19b4dec154b30771890e9ac6a8527ddcc7f6d68e6d29b8",
+ "src/flybrain/backends/__init__.py": "6c94a5cc4be4916e569562041036a6e169b13b228d2bc2dffc0494cff90bd64a",
+ "src/flybrain/backends/cuda.py": "da6f944b0075733d668615ec73f2822cdf740d404d06d2e8309afec1a63b9e9b",
+ "src/flybrain/backends/cpu.py": "20ff6d342efe088f161b800f825175b77b754e42378e7879e6ff5007da5fe11a",
+ "src/flybrain/backends/base.py": "bb28e7aa30f3cb3b676243e6e3767242d5daa27458b5e5a96b218232aaa1de81",
+ "src/flybrain/importers/__init__.py": "d6171134397cf1089cc14419f114172a77863ca90c83bc8cb6780dcb3cc74394",
+ "src/flybrain/importers/malecns.py": "f0b1fda6403807f127eb839510f66cc567143535a479446e66ed8bcfdea6507d",
+ "src/flybrain/data/__init__.py": "6b42fe29f9b5a6f3e309d4fd947212a6a49b92c095e9f815519360dc14924790",
+ "tests/test_session.py": "93007acdb15bd2ad381c5a785b2b69b3879072df81469d07667317b97b2cac7b",
+ "tests/test_open_api.py": "02f5f6ff5d881f5454f2eaf8db4bec42a72050a5c3553379c6490710e924041c",
+ "tests/test_experiment.py": "7fae145cad17b304afa1047237235f1374711143a92bfbcb2a018592caf35e17",
+ "tests/test_sensory.py": "c0c32fb68ac85511ffc04f0ae14a42f83da6775311aabaf391e04164d39b937f",
+ "tests/test_fullbrain_ids.py": "f228517a37cd254249609fa623e4c9ca40129bae96997356cb31948cab489ca5",
+ "tests/test_registry.py": "64cf3bd0f6f6d8faaae7702c31ffacb9ddcaf0ca133fee5905d2ff3bdfd120a1",
+ "tests/test_importers.py": "f3c11167fbb7c709cfd60aa272b43ad868a4cf910f7ec2a035958be8d42b2e66",
+ "tests/test_external.py": "bc5f1327c030e93625e496d3c46aef4a7792a009a68710ec990190185bbb8ac4",
+ "tests/test_projects.py": "5798d3b158383f3eee254a39ea98d88eab7b2b6a7b7d5af3f9aad241cf4ddfda",
+ "tests/test_reflex_training.py": "feac7bbf905809233aa4945807f43338aead5a986652b56798e00003ed6d6dc0",
+ "tests/test_checkpoint.py": "aafe0381b788b72b206eea16011b803acf0ac5b648c1a620760dc8f008c0b2b5",
+ "tests/test_real_model.py": "18124d006083819aecabb3e09fceb2ea5de64c23349f9a72594515ccb50c796e",
+ "tests/test_cuda_hardware.py": "16ec1436ca16842edcc095a249e061ed797c94d644dcf428ee20399fca6bc102",
+ "tests/test_api.py": "8f7b00c9eddc267e0ccba406ab10b1140402c9925b0de9e8c2dcb765c4e634cc",
+ "tests/test_cuda_optional.py": "f12741c1d3bf4b73a5a3562741a3298c33e961ca78a1636593c0edc7279c820c",
+ "tests/test_godot_bridge.py": "d3cabe6917eec93a33d75951463c0a4e3c950228e77e3aa061a63ad4585172b8",
+ "tests/test_cpu.py": "134f9eca25a2693d9c2a8e3c50ee946f434c253f7fe5b288be43d94d64c2be71",
+ "scripts/validate_fullbrain.py": "12bfd2df4a80be66cc75183a75f06eebcb1f46b9a160ed82472f004800cd5d41",
+ "scripts/validate_cuda.py": "113060288dc4865065542870ba102d085785327edcb6a6eb7a781e47a0528dea",
+ "pyproject.toml": "384118ac00ad73f7cca3cd0f3bee19d459525d733a8fd750c3824b1a3a04ec18"
+}
diff --git a/docs/validation/flywire-odor-a16.json b/docs/validation/flywire-odor-a16.json
new file mode 100644
index 0000000..92e613a
--- /dev/null
+++ b/docs/validation/flywire-odor-a16.json
@@ -0,0 +1,15580 @@
+{
+ "schema": "flybrain-full-odor-probe-v1",
+ "status": "completed",
+ "started_utc": "2026-09-13T01:54:21Z",
+ "script_sha256": "a64875638bd8108db66f4057b94df83fef9027ac060e1ec6c553b282beb68600",
+ "config": {
+ "dt_ms": 1.0,
+ "tau_ms": 10.0,
+ "rest": 0.0,
+ "reset": 0.0,
+ "threshold": 1.0,
+ "refractory_ms": 2.0,
+ "input_gain": 2.0,
+ "rate_tau_ms": 50.0,
+ "action_rate_hz": 100.0
+ },
+ "protocol": {
+ "pre_ms": 100,
+ "odor_ms": 500,
+ "post_ms": 200,
+ "trace_interval_ms": 10,
+ "stimulus": "Or42b/DM1 single-channel food-odor approximation",
+ "max_current": 5.0,
+ "half_concentration": 0.2,
+ "background_current": 0.0,
+ "weight_policy": "unchanged full-brain benchmark",
+ "concentration_units": "dimensionless, not a measured chemical dose"
+ },
+ "conditions": [
+ {
+ "name": "no_odor",
+ "antenna_concentrations": [
+ 0.0,
+ 0.0
+ ],
+ "silence_input_neurons": false,
+ "spikes": {
+ "ORN_DM1_left": 0,
+ "ORN_DM1_right": 0,
+ "DNa02_left": 0,
+ "DNa02_right": 0,
+ "ALPN": 0,
+ "MBON": 0,
+ "descending": 0
+ },
+ "trace": [
+ {
+ "time_ms": 10,
+ "groups": {
+ "ORN_DM1_left": {
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+ "max_voltage": 0.0
+ },
+ "ORN_DM1_right": {
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+ "max_voltage": 0.0
+ },
+ "DNa02_left": {
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+ "max_voltage": 0.0
+ },
+ "DNa02_right": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "ALPN": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "MBON": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "descending": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ }
+ }
+ },
+ {
+ "time_ms": 20,
+ "groups": {
+ "ORN_DM1_left": {
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+ "max_voltage": 0.0
+ },
+ "ORN_DM1_right": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "DNa02_left": {
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+ "max_voltage": 0.0
+ },
+ "DNa02_right": {
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+ "max_voltage": 0.0
+ },
+ "ALPN": {
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+ "max_voltage": 0.0
+ },
+ "MBON": {
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+ "max_voltage": 0.0
+ },
+ "descending": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ }
+ }
+ },
+ {
+ "time_ms": 30,
+ "groups": {
+ "ORN_DM1_left": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "ORN_DM1_right": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "DNa02_left": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "DNa02_right": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "ALPN": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "MBON": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "descending": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ }
+ }
+ },
+ {
+ "time_ms": 40,
+ "groups": {
+ "ORN_DM1_left": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "ORN_DM1_right": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "DNa02_left": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "DNa02_right": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "ALPN": {
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+ "max_voltage": 0.0
+ },
+ "MBON": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "descending": {
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+ "max_voltage": 0.0
+ }
+ }
+ },
+ {
+ "time_ms": 50,
+ "groups": {
+ "ORN_DM1_left": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "ORN_DM1_right": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "DNa02_left": {
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+ "max_voltage": 0.0
+ },
+ "DNa02_right": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "ALPN": {
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+ "max_voltage": 0.0
+ },
+ "MBON": {
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+ "max_voltage": 0.0
+ },
+ "descending": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ }
+ }
+ },
+ {
+ "time_ms": 60,
+ "groups": {
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+ "max_voltage": 0.0
+ },
+ "ORN_DM1_right": {
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+ "max_voltage": 0.0
+ },
+ "DNa02_left": {
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+ "max_voltage": 0.0
+ },
+ "DNa02_right": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "ALPN": {
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+ "max_voltage": 0.0
+ },
+ "MBON": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "descending": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ }
+ }
+ },
+ {
+ "time_ms": 70,
+ "groups": {
+ "ORN_DM1_left": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "ORN_DM1_right": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "DNa02_left": {
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+ "max_voltage": 0.0
+ },
+ "DNa02_right": {
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+ "max_voltage": 0.0
+ },
+ "ALPN": {
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+ "max_voltage": 0.0
+ },
+ "MBON": {
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+ "max_voltage": 0.0
+ },
+ "descending": {
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+ "max_voltage": 0.0
+ }
+ }
+ },
+ {
+ "time_ms": 80,
+ "groups": {
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+ "max_voltage": 0.0
+ },
+ "ORN_DM1_right": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "DNa02_left": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "DNa02_right": {
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+ "max_voltage": 0.0
+ },
+ "ALPN": {
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+ "max_voltage": 0.0
+ },
+ "MBON": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "descending": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ }
+ }
+ },
+ {
+ "time_ms": 90,
+ "groups": {
+ "ORN_DM1_left": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "ORN_DM1_right": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "DNa02_left": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "DNa02_right": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "ALPN": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "MBON": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "descending": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ }
+ }
+ },
+ {
+ "time_ms": 100,
+ "groups": {
+ "ORN_DM1_left": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "ORN_DM1_right": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "DNa02_left": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "DNa02_right": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "ALPN": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "MBON": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "descending": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ }
+ }
+ },
+ {
+ "time_ms": 110,
+ "groups": {
+ "ORN_DM1_left": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "ORN_DM1_right": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "DNa02_left": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "DNa02_right": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "ALPN": {
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+ "max_voltage": 0.0
+ },
+ "MBON": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "descending": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ }
+ }
+ },
+ {
+ "time_ms": 120,
+ "groups": {
+ "ORN_DM1_left": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "ORN_DM1_right": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "DNa02_left": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "DNa02_right": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "ALPN": {
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+ "max_voltage": 0.0
+ },
+ "MBON": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "descending": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ }
+ }
+ },
+ {
+ "time_ms": 130,
+ "groups": {
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+ "max_voltage": 0.0
+ },
+ "ORN_DM1_right": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "DNa02_left": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "DNa02_right": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "ALPN": {
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+ "max_voltage": 0.0
+ },
+ "MBON": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "descending": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ }
+ }
+ },
+ {
+ "time_ms": 140,
+ "groups": {
+ "ORN_DM1_left": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "ORN_DM1_right": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "DNa02_left": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "DNa02_right": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
+ },
+ "ALPN": {
+ "mean_rate_hz": 0.0,
+ "max_voltage": 0.0
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+ ]
+ },
+ "counts": {
+ "ORN_DM1_left": 35,
+ "ORN_DM1_right": 33,
+ "DNa02_left": 1,
+ "DNa02_right": 1,
+ "ALPN": 685,
+ "MBON": 96,
+ "descending": 1303
+ }
+ },
+ "device": {
+ "name": "NVIDIA A16-8Q",
+ "memory_bytes": 8394047488,
+ "cupy": "14.2.0",
+ "driver": 12040,
+ "runtime": 12090
+ },
+ "load_seconds": 67.89835428700002,
+ "cuda_kernel_sha256": "da6f944b0075733d668615ec73f2822cdf740d404d06d2e8309afec1a63b9e9b",
+ "findings": {
+ "input_stimulation_and_silencing_checks": "passed",
+ "bilateral_descending_spikes": 0,
+ "interpretation": "Presence of descending spikes does not prove odor-directed navigation; absence means this preset has not demonstrated the required motor response. No behavior, banana recognition, flight or learning was tested here."
+ },
+ "finished_utc": "2026-09-13T01:56:35Z"
+}
diff --git a/docs/validation/flywire-odor-a16.md b/docs/validation/flywire-odor-a16.md
new file mode 100644
index 0000000..f1b03d3
--- /dev/null
+++ b/docs/validation/flywire-odor-a16.md
@@ -0,0 +1,78 @@
+# Full FlyWire odor probe — input works, downstream spiking absent
+
+**Negative functional result, 2026-09-13 UTC.** All 139,255 neurons and 16,847,997
+source rows ran on NVIDIA A16-8Q. This is not a subgraph. CUDA's 12 hardware cases
+passed; the full suite passed **181 tests, zero skips**. The five-condition
+experiment completed, but this preset did **not** demonstrate odor transmission
+through the olfactory projection neurons or motor output.
+
+| Condition | Input ORN spikes | ALPN spikes | MBON spikes | Descending spikes |
+|---|---:|---:|---:|---:|
+| no_odor | 0 | 0 | 0 | 0 |
+| left_odor | 3,500 | 0 | 0 | 0 |
+| right_odor | 3,300 | 0 | 0 | 0 |
+| bilateral_odor | 6,800 | 0 | 0 | 0 |
+| bilateral_odor_ORNs_silenced | 0 | 0 | 0 | 0 |
+
+Each condition: 100 ms rest + 500 ms stimulus + 200 ms recovery, from the same
+resting checkpoint. No global background current. Only annotated ORN_DM1 cells
+receive external input. Left/right cell counts are 35/33. A single responsive
+Or42b channel is approximated; no complete banana mixture is claimed.
+
+The maximum observed ALPN membrane potential was 0.2516453 (threshold 1).
+Maximum descending membrane potential was 0.00129747. Zero spikes therefore
+means no threshold crossing; it does not mean absolutely no subthreshold signal.
+The GPU executed 4,000 total ticks. Per-condition wall times were 9.62–9.69 s,
+including selected-neuron observations; this was not a throughput benchmark.
+
+## Why this preset cannot support the requested behavior
+
+The historical numerical benchmark normalized every neuron's incoming absolute
+weight sum to 2.5. With its 1 ms step, 10 ms membrane time constant and two-tick
+refractory hold, an input cell can spike at most once every three ticks. From
+rest and with zero external postsynaptic current, the largest possible voltage
+is bounded by the all-excitatory, perfectly synchronized case:
+
+```
+leak = exp(-1/10)
+V_max <= 2.5 * (1 - leak) / (1 - leak**3) = 0.9179135 < threshold 1
+```
+
+Inhibition only lowers this upper bound. Thus increasing odor intensity alone
+cannot fix transmission in this preset. A separate CPU two-cell diagnostic with
+maximal 3-tick firing spacing reached 0.9179135027773137 and zero downstream
+spikes over 2,000 ticks, matching the independent bound. That small diagnostic
+explains the parameter issue; it is not a substitute for the full-brain GPU run.
+The original full-brain benchmark remains valid for numerical conformance,
+checkpointing and timing under its artificial global input, **not physiology**.
+
+## Next implementation gate
+
+Use an explicitly separate dynamics configuration based on the published
+[Shiu et al. model](https://www.nature.com/articles/s41586-024-07763-9), rather than
+silently changing the historical benchmark. Its membrane and synaptic states,
+synaptic decay, transmission delay, refractory behavior and Poisson sensory
+stimulation must be checked against an independent reference before making
+behavior claims. Preserve the full graph, source/weight provenance and the same
+negative controls. Only after propagation is demonstrated should a voxel-world
+body consume neural outputs. Any learned or handcrafted readout must be labeled
+as such; target coordinates must not bypass the neural path.
+
+## Evidence and reproduction
+
+- [Protocol, annotation download and commands](../olfactory-experiment.md).
+- [Raw report with all 400 sampled frames](flywire-odor-a16.json).
+- [Actual GPU test suite XML](flywire-odor-pytest.xml).
+- [Tested Python source SHA-256 values](flywire-odor-source-sha256.txt).
+- [Analytical-bound CPU diagnostic](benchmark-voltage-bound.json).
+- Tested source: `357e6546cad18b45b7033c9725c140d0b8901dec`. The subsequent
+ `930b5f9` commit only formats an annotation metadata string.
+- Downloaded evidence archive SHA-256:
+ `e41c10b70bf46a51569afb3e404213f5ee01924adcc8e2d324380d7dd82881aa`.
+ All source hashes were matched to the published tested commit.
+- CUDA backend/kernel unchanged from the earlier numerical benchmark. CuPy
+ 14.2.0, CUDA runtime API 12090, driver API 12040, 8,394,047,488 device bytes.
+
+The temporary GPU instance was terminated after results were retrieved and
+verified. No persistent GPU endpoint or live browser inference is provided by
+this report.
diff --git a/docs/validation/flywire-odor-pytest.xml b/docs/validation/flywire-odor-pytest.xml
new file mode 100644
index 0000000..b28b61b
--- /dev/null
+++ b/docs/validation/flywire-odor-pytest.xml
@@ -0,0 +1 @@
+
\ No newline at end of file
diff --git a/docs/validation/flywire-odor-source-sha256.txt b/docs/validation/flywire-odor-source-sha256.txt
new file mode 100644
index 0000000..16db212
--- /dev/null
+++ b/docs/validation/flywire-odor-source-sha256.txt
@@ -0,0 +1,37 @@
+0e04b67cc0f32f4d5b109b2e8bfdb9082d0f27e295df2718b5a597f8a2e3192a source/src/flybrain/session.py
+ad45e3a91920affae56b74f2b3d2d1d0b8eab41d705acf3ccd4fbba8b8a5cee6 source/src/flybrain/demos.py
+e9ea19aea644bcbdcc686b6e79ee5767f4c620cdeb063cf69fd7a014a87e9450 source/src/flybrain/brain.py
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diff --git a/docs/validation/flywire-synaptic-odor-a16.json b/docs/validation/flywire-synaptic-odor-a16.json
new file mode 100644
index 0000000..e13360f
--- /dev/null
+++ b/docs/validation/flywire-synaptic-odor-a16.json
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+ "720575940647029044",
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+ "720575940647228468",
+ "720575940647471395",
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+ "720575940647560500",
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+ "720575940647755641",
+ "720575940647964281",
+ "720575940648180868",
+ "720575940648188548",
+ "720575940648636292",
+ "720575940648654468",
+ "720575940649809273",
+ "720575940649829241",
+ "720575940649992569",
+ "720575940650282742",
+ "720575940650929273",
+ "720575940651341558",
+ "720575940651932150",
+ "720575940652104694",
+ "720575940653053601",
+ "720575940653310198",
+ "720575940653614838",
+ "720575940654094241",
+ "720575940655587489",
+ "720575940656144289",
+ "720575940659356545",
+ "720575940659703937",
+ "720575940660220289",
+ "720575940660224385",
+ "720575940660229505",
+ "720575940660269185"
+ ]
+ },
+ "counts": {
+ "ORN_DM1_left": 35,
+ "ORN_DM1_right": 33,
+ "DNa02_left": 1,
+ "DNa02_right": 1,
+ "ALPN": 685,
+ "MBON": 96,
+ "descending": 1303
+ }
+ },
+ "device": {
+ "name": "NVIDIA A16-8Q",
+ "memory_bytes": 8394047488,
+ "cupy": "14.2.0",
+ "driver": 12040,
+ "runtime": 12090
+ },
+ "full_graph_cpu_cuda_parity": {
+ "ticks": 200,
+ "observations": [
+ {
+ "tick": 40,
+ "errors": {
+ "voltage_mv": 0.0,
+ "synaptic_mv": 0.0,
+ "rates_hz": 0.0
+ }
+ },
+ {
+ "tick": 80,
+ "errors": {
+ "voltage_mv": 0.0,
+ "synaptic_mv": 0.0,
+ "rates_hz": 0.0
+ }
+ },
+ {
+ "tick": 120,
+ "errors": {
+ "voltage_mv": 0.0,
+ "synaptic_mv": 0.0,
+ "rates_hz": 0.0
+ }
+ },
+ {
+ "tick": 160,
+ "errors": {
+ "voltage_mv": 0.0,
+ "synaptic_mv": 0.0,
+ "rates_hz": 0.0
+ }
+ },
+ {
+ "tick": 200,
+ "errors": {
+ "voltage_mv": 0.0,
+ "synaptic_mv": 0.0,
+ "rates_hz": 0.0
+ }
+ }
+ ],
+ "input_events": 201,
+ "status": "passed",
+ "json_checkpoint_replay_ticks": 20,
+ "wall_seconds": 27.289984679999975
+ },
+ "findings": {
+ "input_and_silencing_controls": "passed",
+ "bilateral_descending_spikes": 2883,
+ "interpretation": "Report includes absent responses. Descending spikes alone do not prove odor-directed navigation. No banana recognition, flight or learning tested."
+ },
+ "finished_utc": "2026-09-13T03:01:55Z"
+}
diff --git a/docs/validation/flywire-synaptic-odor-a16.md b/docs/validation/flywire-synaptic-odor-a16.md
new file mode 100644
index 0000000..43fe1e5
--- /dev/null
+++ b/docs/validation/flywire-synaptic-odor-a16.md
@@ -0,0 +1,90 @@
+# Complete FlyWire synaptic CUDA odor experiment
+
+**Sensory propagation observed, 2026-09-13 UTC. Navigation not established.**
+The separate mV synaptic engine ran all **139,255 neurons**, retaining all
+**16,847,997 aggregate edges / 54,492,922 anatomical contacts**, without pruning.
+The historical normalized benchmark and its negative result remain unchanged.
+
+| Condition | ORN left / right spikes | ALPN | MBON | Descending | DNa02 left / right |
+|---|---:|---:|---:|---:|---:|
+| No odor | 0 / 0 | 0 | 0 | 0 | 0 / 0 |
+| Left odor | 2,545 / 0 | 23,298 | 3,286 | 2,778 | 29 / 6 |
+| Right odor | 0 / 2,466 | 23,305 | 3,175 | 2,799 | 32 / 8 |
+| Bilateral odor | 2,545 / 2,466 | 23,503 | 3,276 | 2,883 | 32 / 9 |
+| Bilateral, ORNs silenced | 0 / 0 | 0 | 0 | 0 | 0 / 0 |
+
+Only annotated DM1 sensory cells received external input. Each condition started
+from the same resting checkpoint: 100 ms rest, 500 ms stimulus, 200 ms recovery;
+dt=0.1 ms, 8,000 steps per condition. NumPy PCG64 seed 20260913 draws are matched
+across conditions. Input uses the discrete PoissonGroup approximation p=rate*dt,
+150 Hz nominal rate and 68.75 mV jumps. Input events can be cleared by same-tick
+reset, so injected events and observed ORN spikes are intentionally distinct.
+
+## What the results establish
+
+- Five actual-GPU conformance cases executed: **zero skipped, failed or errored**.
+ These cover CPU parity, delays, inhibition, equal decay constants, stream
+ ownership, cross-backend checkpoints, silencing and overflow rollback.
+- The complete graph passed **200 active CPU/GPU ticks** with 201 sensory input
+ events. At five checkpoints, every neuron's voltage, synaptic state and rate
+ had maximum absolute difference **0**. Spike/history/refractory timestamps
+ were exactly equal. This covers the measured trajectory, not all possible inputs.
+- Full-graph JSON state plus input RNG replayed **20 future ticks exactly**.
+- The same hardware environment passed **204 software tests, zero skips**.
+- Sensory-only stimulation now drives ALPN, MBON and descending spikes. The
+ no-odor and input-silenced controls remain silent in these recorded populations.
+
+## What remains unresolved
+
+DNa02 activity is left-biased for both left and right stimulation. The observed
+pair therefore does not simply encode stimulus side. Counts in aggregate output
+populations are not proof of correct body commands, attraction or reliable
+navigation. This protocol has one seed, nominal parameters and an 800 ms horizon;
+it does not establish robust physiology across doses, seeds or behavioral states.
+
+Time matters: at 150 ms (50 ms after stimulus onset), the right-odor DNa02
+rates are left 18.15 Hz / right 34.07 Hz, whereas left odor gives 32.84 / 0 Hz.
+Thus the cumulative left bias does **not** establish absence of all transient
+side information. The current raw rate-difference motor mapping still needs
+closed-loop evaluation rather than an inference from these early samples.
+
+Activity also persists after stimulus offset. In the 600–800 ms window, the
+bilateral condition adds 6,842 ALPN, 924 MBON and 859 descending spikes. These
+are new spike counts, not just the tail of the rate estimator. The finite
+recording does not establish indefinite persistence, biological memory or its
+mechanism. Longer recovery and recurrent-gain/transmitter assumptions need
+checking before calling this a calibrated sensorimotor model. See the
+[derived post-stimulus counts](synaptic-post-stimulus.json), linked by raw-report hash.
+
+The graph uses assumed transmitter signs, 0.275 mV/contact weights and no incoming
+normalization. These fitted research parameters do not measure every synapse.
+The [independent Brian2 validation](synaptic-brian2-reference.json) establishes
+the implemented equations/schedule, not biological validity of this particular
+graph/parameter combination. The stimulus is one Or42b/DM1 channel, not a complete
+ethyl-acetate response, banana mixture or visual-recognition model.
+
+Each 800 ms condition took 102.1–104.1 seconds, including observations. These
+are experiment wall times, not an isolated kernel benchmark or real-time claim.
+The raw report's `mapping.stimulus.current_units` describes the historical
+dimensionless adapter bundled with the annotation map; it is not used here.
+The actual mV input units, dynamics and weights are defined in `protocol`,
+`config` and `model.weight_units`.
+
+## Evidence and reproduction
+
+- [Raw five-condition report](flywire-synaptic-odor-a16.json)
+- [Five-case actual CUDA gate](synaptic-cuda-a16.json)
+- [Software test XML](synaptic-gpu-pytest.xml)
+- [64 tested Python file SHA-256 values](synaptic-gpu-source-sha256.txt)
+- [Reproduction commands and physical assumptions](../synaptic-dynamics.md)
+- Tested source: `592738a3a963961e81a6cc948d0b5d3dfca3471a`.
+- Graph arrays SHA-256:
+ `f6dc0d6348cc7db3be7514323a57142549327e679f9aee709b2343351685f5b5`.
+- Retrieved evidence archive SHA-256:
+ `cfeaabc6fb28b90f76b2e4cd0ac013c7cf3a87d44d1e813141d5474e181d9a47`.
+- NVIDIA A16-8Q, 8,394,047,488 bytes device memory; CuPy 14.2.0;
+ CUDA driver API 12040 / runtime API 12090; Python 3.12.3, NumPy 2.5.3.
+- Started `2026-09-13T02:51:35Z`; finished `2026-09-13T03:01:55Z`.
+
+The archive checksum and all 64 recorded source file hashes were verified after
+download. The source graph files also passed the pinned official checksums.
diff --git a/docs/validation/flywire-voxel-a16.md b/docs/validation/flywire-voxel-a16.md
new file mode 100644
index 0000000..faa0e1b
--- /dev/null
+++ b/docs/validation/flywire-voxel-a16.md
@@ -0,0 +1,69 @@
+# Full-brain CUDA voxel experiment: movement, no successful foraging
+
+**Recorded on NVIDIA A16-8Q, 2026-09-13.** All 139,255 FlyWire v783 neurons and
+16,847,997 aggregate edges were simulated on the GPU in both runs. A fixed,
+untrained readout converted DNa02 rates to a kinematic ground body's speed and
+turn. There was no target-bearing controller or direct motor stimulation.
+
+| Recorded condition | Simulated time | Path length | Initial / final food distance | Food contact |
+|---|---:|---:|---:|---|
+| Neural control | 2 s | 3.840 | 2.778 / 4.380 | No |
+| ORN inputs silenced | 2 s | 0 | 2.778 / 2.778 | No |
+
+Distances use arbitrary game units. The active run initially approached to
+2.266 units, then curved left and moved away. It did not reach the 0.6-unit
+contact radius. Neither run encountered a block; collision mechanics are tested
+separately in the CPU environment tests, not established by these trajectories.
+This is evidence of an implemented neural-to-body closed loop, **not successful
+odor-directed navigation, training or biological locomotion**.
+
+## Exact control boundary
+
+- World observations contain only left and right local antenna concentrations.
+- Input rates are `180*c/(0.2+c)` Hz; per-tick Bernoulli events generate 68.75 mV
+ jumps on the 35 left / 33 right annotated DM1 input cells.
+- All graph cells update at 0.1 ms. After 200 neural steps, the 20 ms body frame
+ consumes DNa02 left/right exponentially smoothed rates, with a 50 ms time constant.
+- Speed = `clip(0.03*(left+right), 0, 3)` game units/s.
+- Turn = `2*tanh((right-left)/40)` radians/s. Positive turns toward world +z.
+- Food coordinates reach the odor field, rendering and scoring only, never the
+ motor readout. Input silencing operates on the brain, not on renderer animation.
+
+The speed mapping is an engineering hypothesis with no biological validation.
+The ground body, Gaussian field, gains, initial scene and seed are explicit in
+the recording. The model covers one food-odor channel and no vision. DNa02's
+side bias and post-stimulus persistence in the [sensory controls](flywire-synaptic-odor-a16.md)
+remain reasons to investigate calibration and a more informative readout.
+
+## Validation and reproduction
+
+- **207 software tests passed on the GPU host, zero skips**:
+ [test XML](voxel-gpu-pytest.xml).
+- At frame 20 in each run, brain state (including all delayed events), world and
+ input RNG were serialized through JSON. The next complete 20 ms frame was
+ replayed and compared: exact full-brain state, world record and RNG equality.
+- All 100 frames per run are preserved. Wall times: active 243.654 s; silenced
+ 248.907 s. These include observations and the extra replay checks; not real time.
+- Tested source: `e89b8feaf7f2381eeaad625de0cc0897bb6ac773`.
+- [Tested Python source hashes](voxel-gpu-source-sha256.txt) and the hashes in the
+ raw recording were verified after download.
+- Evidence archive SHA-256:
+ `4e3dc17aa64c878c3447320ad9098f94a93a79c05655fb710f21f8b64af64455`.
+- Started `2026-09-13T03:04:58Z`; finished `2026-09-13T03:14:25Z`.
+- NVIDIA A16-8Q / CuPy 14.2.0 / CUDA driver API 12040 / runtime API 12090.
+
+```bash
+python scripts/run_fullbrain_voxel.py --data-dir data \
+ --annotations data/annotations.tsv --output fullbrain-voxel.json
+```
+
+Use the source files and dependencies from the [full-brain odor protocol](../olfactory-experiment.md).
+[The browser page](https://freeman-1984-coder.github.io/flybrain-sdk/foraging.html)
+replays the [complete JSON recording](../../site/experiments/fullbrain-voxel.json).
+It supports selecting the control, scrubbing time and rotating the view; it does
+not perform live GPU inference. It refuses to substitute generated trajectories
+when the recording is absent or incomplete.
+
+The temporary GPU instance was destroyed after evidence retrieval and checksum
+verification. The provider instance list was confirmed empty; the task SSH key
+was deleted. The shared temporary rental for these experiments cost $0.24.
diff --git a/docs/validation/full-suite.xml b/docs/validation/full-suite.xml
new file mode 100644
index 0000000..21e1108
--- /dev/null
+++ b/docs/validation/full-suite.xml
@@ -0,0 +1 @@
+('/root/flybrain-test/tests/test_importers.py', 6, "Skipped: could not import 'pyarrow': No module named 'pyarrow'")
\ No newline at end of file
diff --git a/docs/validation/reflex-cuda.json b/docs/validation/reflex-cuda.json
new file mode 100644
index 0000000..b8f1e95
--- /dev/null
+++ b/docs/validation/reflex-cuda.json
@@ -0,0 +1,2414 @@
+{
+ "format": "flybrain-reflex-training-v1",
+ "backend": "cuda",
+ "model": "male-cns-escape-v1",
+ "neurons": 313,
+ "model_fingerprint": "47a0c91b3eba9c606c29faef58fe15c8846fc049c4314a5e3b6912ab22c5e68a",
+ "checkpoint": {
+ "format": "flybrain-external-reflex-readout-v1",
+ "neuron_ids": [
+ "10010",
+ "10001"
+ ],
+ "scale_hz": 100,
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+ "left",
+ "right"
+ ]
+ },
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+ "seconds": 33.872531293000065,
+ "limitations": [
+ "Fixed real anatomical subgraph; LIF parameters and input mapping assumed.",
+ "Only the external three-weight action readout is learned.",
+ "Two-choice 80ms obstacle task; trials reset neural state.",
+ "Held-out trials vary stimulus amplitude, not task or environment.",
+ "Untrained zero weights choose right on ties; chance baseline is 50%.",
+ "No claim of biological learning, whole-fly intelligence or broad generalization."
+ ]
+}
diff --git a/docs/validation/requirements-tested.txt b/docs/validation/requirements-tested.txt
new file mode 100644
index 0000000..b3d8310
--- /dev/null
+++ b/docs/validation/requirements-tested.txt
@@ -0,0 +1,19 @@
+cuda-pathfinder==1.8.1
+cuda-toolkit==12.9.2.0
+cupy-cuda12x==14.2.0
+# Editable install with no version control (flybrain-sdk==0.5.0.dev0)
+-e /root/flybrain-test
+iniconfig==2.3.0
+numpy==2.2.6
+nvidia-cublas-cu12==12.9.2.10
+nvidia-cuda-nvrtc-cu12==12.9.86
+nvidia-cuda-runtime-cu12==12.9.79
+nvidia-cufft-cu12==11.4.1.4
+nvidia-curand-cu12==10.3.10.19
+nvidia-cusolver-cu12==11.7.5.82
+nvidia-cusparse-cu12==12.5.10.65
+nvidia-nvjitlink-cu12==12.9.86
+packaging==26.3
+pluggy==1.6.0
+Pygments==2.21.0
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diff --git a/docs/validation/synaptic-brian2-reference.json b/docs/validation/synaptic-brian2-reference.json
new file mode 100644
index 0000000..fd091e7
--- /dev/null
+++ b/docs/validation/synaptic-brian2-reference.json
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+{
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diff --git a/docs/validation/synaptic-cuda-a16.json b/docs/validation/synaptic-cuda-a16.json
new file mode 100644
index 0000000..1c34432
--- /dev/null
+++ b/docs/validation/synaptic-cuda-a16.json
@@ -0,0 +1,24 @@
+{
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+ "source_sha256": {
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+ "scripts/validate_synaptic_cuda.py": "7f51e7147d8c51d879a663e85841077197196851648e780d05fb86eceaf13c80"
+ },
+ "device": {
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+ "runtime": 12090
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+ "failures": 0
+ }
+}
diff --git a/docs/validation/synaptic-gpu-pytest.xml b/docs/validation/synaptic-gpu-pytest.xml
new file mode 100644
index 0000000..016b047
--- /dev/null
+++ b/docs/validation/synaptic-gpu-pytest.xml
@@ -0,0 +1 @@
+
\ No newline at end of file
diff --git a/docs/validation/synaptic-gpu-source-sha256.txt b/docs/validation/synaptic-gpu-source-sha256.txt
new file mode 100644
index 0000000..6a3fbab
--- /dev/null
+++ b/docs/validation/synaptic-gpu-source-sha256.txt
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+feac7bbf905809233aa4945807f43338aead5a986652b56798e00003ed6d6dc0 tests/test_reflex_training.py
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+c0c32fb68ac85511ffc04f0ae14a42f83da6775311aabaf391e04164d39b937f tests/test_sensory.py
+93007acdb15bd2ad381c5a785b2b69b3879072df81469d07667317b97b2cac7b tests/test_session.py
+acbe6ccd889bb800cd84cc2cf95f9d7d7e015f8449192f311e7d008d22949ee7 tests/test_synaptic.py
+70761bdbb7cbd4fdaa82ea85b40494a84fb532d9d6af350449aa33e46219bb1e tests/test_synaptic_cuda_hardware.py
+284e10f5db01293ad85f16d1a815e029880aaf1e3db7479917bcc7cb9ae6eefa tests/test_synaptic_odor_protocol.py
diff --git a/docs/validation/synaptic-post-stimulus.json b/docs/validation/synaptic-post-stimulus.json
new file mode 100644
index 0000000..afd1e63
--- /dev/null
+++ b/docs/validation/synaptic-post-stimulus.json
@@ -0,0 +1,70 @@
+{
+ "source_sha256": "0072f44c17dbc0eb3854a3d509d037aaa5dc04ec1ed9a46c30c511f808911c6c",
+ "window_ms": [
+ 600,
+ 800
+ ],
+ "definition": "cumulative spikes at 800ms minus at 600ms; input stimulus ends at 600ms",
+ "conditions": [
+ {
+ "name": "no_odor",
+ "post_stimulus_spikes": {
+ "ORN_DM1_left": 0,
+ "ORN_DM1_right": 0,
+ "DNa02_left": 0,
+ "DNa02_right": 0,
+ "ALPN": 0,
+ "MBON": 0,
+ "descending": 0
+ }
+ },
+ {
+ "name": "left_odor",
+ "post_stimulus_spikes": {
+ "ORN_DM1_left": 1,
+ "ORN_DM1_right": 0,
+ "DNa02_left": 8,
+ "DNa02_right": 3,
+ "ALPN": 6782,
+ "MBON": 934,
+ "descending": 799
+ }
+ },
+ {
+ "name": "right_odor",
+ "post_stimulus_spikes": {
+ "ORN_DM1_left": 0,
+ "ORN_DM1_right": 2,
+ "DNa02_left": 11,
+ "DNa02_right": 2,
+ "ALPN": 6887,
+ "MBON": 924,
+ "descending": 832
+ }
+ },
+ {
+ "name": "bilateral_odor",
+ "post_stimulus_spikes": {
+ "ORN_DM1_left": 1,
+ "ORN_DM1_right": 2,
+ "DNa02_left": 11,
+ "DNa02_right": 1,
+ "ALPN": 6842,
+ "MBON": 924,
+ "descending": 859
+ }
+ },
+ {
+ "name": "bilateral_odor_ORNs_silenced",
+ "post_stimulus_spikes": {
+ "ORN_DM1_left": 0,
+ "ORN_DM1_right": 0,
+ "DNa02_left": 0,
+ "DNa02_right": 0,
+ "ALPN": 0,
+ "MBON": 0,
+ "descending": 0
+ }
+ }
+ ]
+}
diff --git a/docs/validation/voxel-gpu-pytest.xml b/docs/validation/voxel-gpu-pytest.xml
new file mode 100644
index 0000000..e04e29f
--- /dev/null
+++ b/docs/validation/voxel-gpu-pytest.xml
@@ -0,0 +1 @@
+
\ No newline at end of file
diff --git a/docs/validation/voxel-gpu-source-sha256.txt b/docs/validation/voxel-gpu-source-sha256.txt
new file mode 100644
index 0000000..8ccf0ea
--- /dev/null
+++ b/docs/validation/voxel-gpu-source-sha256.txt
@@ -0,0 +1,67 @@
+de9596a06a4de0a04303795a10ae0a6d8cbbc7705a5e60ad566b4c1bfad65971 src/flybrain/__init__.py
+c125d73bf421cac3c6a8f4185ae56516db20cc91831e006c42c544c7b873dfb9 src/flybrain/__main__.py
+6c94a5cc4be4916e569562041036a6e169b13b228d2bc2dffc0494cff90bd64a src/flybrain/backends/__init__.py
+bb28e7aa30f3cb3b676243e6e3767242d5daa27458b5e5a96b218232aaa1de81 src/flybrain/backends/base.py
+20ff6d342efe088f161b800f825175b77b754e42378e7879e6ff5007da5fe11a src/flybrain/backends/cpu.py
+da6f944b0075733d668615ec73f2822cdf740d404d06d2e8309afec1a63b9e9b src/flybrain/backends/cuda.py
+e9ea19aea644bcbdcc686b6e79ee5767f4c620cdeb063cf69fd7a014a87e9450 src/flybrain/brain.py
+a7f274ce09c28701db19b4dec154b30771890e9ac6a8527ddcc7f6d68e6d29b8 src/flybrain/config.py
+6b42fe29f9b5a6f3e309d4fd947212a6a49b92c095e9f815519360dc14924790 src/flybrain/data/__init__.py
+ad45e3a91920affae56b74f2b3d2d1d0b8eab41d705acf3ccd4fbba8b8a5cee6 src/flybrain/demos.py
+b018bcf2c820ef58ab160204890b465c0702bfba6ca75c65f2d1b33ebb9cac24 src/flybrain/drive.py
+fcded1de58450030889fa069c4fa9f924344accc92418f8e93099639744f2b0d src/flybrain/errors.py
+ae423d017ad86e5fd1d2c49c6c33b73c6d2a339a7e299c25d1195cb2ac10d03c src/flybrain/experiment.py
+570d34f551fd63b33bff564c022ad01fc463b0e1649d9f7035e361b421533bd5 src/flybrain/experimental/__init__.py
+4c3b7b48e659314a1dd839819ddce4bdd1622d21dd8753400035c1df8deaa726 src/flybrain/experimental/synaptic.py
+8b0bc40f8bf0075e6d81caadf3393673110aa48b66415303a08020cb9055dfa8 src/flybrain/experimental/synaptic_cuda.py
+d358df33830ca4d96fe8c5c8549cef383760213d00f8e153f64e2b9c5e7484b3 src/flybrain/experimental/voxel_arena.py
+8b853fe406607bc13c2680e3d686c0b6ca6fc470328a0dbcdce4590f72585f34 src/flybrain/external.py
+d6171134397cf1089cc14419f114172a77863ca90c83bc8cb6780dcb3cc74394 src/flybrain/importers/__init__.py
+f0b1fda6403807f127eb839510f66cc567143535a479446e66ed8bcfdea6507d src/flybrain/importers/malecns.py
+4ee80b0dc5cc9b8d31067b7817e42b273660cdd7b2ac469eae7146a027824173 src/flybrain/model.py
+7dac900576636fb267e5a4a9578731bf5b541e8ea5baf747476fe56db29c8026 src/flybrain/motor.py
+9cfb9c65e7940ec3e930273a00b15417615a486a348aef362ec4760067233690 src/flybrain/neurons.py
+6e7b3c5cba59888efaa4b1c5f2d3da5bde4f70c5f4a6dca2be86d6fcba15e81d src/flybrain/olfaction.py
+ec85f320ac654d11da885d72825ede716b25be2d6d1d7d91795e78db8252a3f2 src/flybrain/projects.py
+503d2eb6e9124b3c2fe7452f4ca8edd1f5128d135a0a406d1c902bf01cb0ef81 src/flybrain/registry.py
+13403eb880fc7c04496d8f5a0405d7b7c116f19b0a69887dff25368c5d291dd3 src/flybrain/sensory.py
+0e04b67cc0f32f4d5b109b2e8bfdb9082d0f27e295df2718b5a597f8a2e3192a src/flybrain/session.py
+26d71040003ac8deff35bd5c0fb42be9d47a0af49a41754795b20fb608910cad src/flybrain/viewer.py
+72f40a0088e457a2999ec4259824a8ca5237bb3c4e31dd0b76d4ec06f8985741 scripts/benchmark.py
+670715224b190463168b87d120d7e5ea26a9da042f400f423ea3ac84e2862760 scripts/browser_reference.py
+d7f7da01ac274fd558eaa02622fa718a743301fd74dd6351387722746e85f8b0 scripts/build_demo_gallery.py
+2294dd1b4c3bd1af186beae6a9f5c726e5356433fe84e51b97f4fd0fda44d4a4 scripts/build_malecns_escape.py
+00c87876ce4dd1475f225333ea5776b33f1473da6d98157f21f3fd3f98efa8ae scripts/build_olfactory_map.py
+41f5640d0452cf570be5e2befd7d82222a209eb07c582e023afd939837e3bccd scripts/dodge_reference.py
+a64875638bd8108db66f4057b94df83fef9027ac060e1ec6c553b282beb68600 scripts/probe_fullbrain_odor.py
+393ae10e825cff56b58cd80f2ab8e559b55cec600886ae85131427545d1cffdf scripts/probe_synaptic_odor.py
+bfbd891cc676ff8f2013d2997b01b1136ac988088f3f02fce236cd4a615ea58f scripts/run_fullbrain_voxel.py
+113060288dc4865065542870ba102d085785327edcb6a6eb7a781e47a0528dea scripts/validate_cuda.py
+a846c281148d043de6c57569b393125e456826281a3e387740f4242b57ed74cc scripts/validate_cuda_modal.py
+4d543ed43e38cbed609d0cd54003f76049347844d7fa8af58d1ac25753157370 scripts/validate_fullbrain.py
+7f51e7147d8c51d879a663e85841077197196851648e780d05fb86eceaf13c80 scripts/validate_synaptic_cuda.py
+9bafd4835bb0824009dc3e9eca9a32a7110ebe54a767779202301a72eb2f80f1 scripts/validate_synaptic_reference.py
+941c1dcfabe38af07b3e6a3b5fabb4b2a89a2cd12ebe81e8ee13c40eb18ec142 scripts/verify_godot.py
+68ecbcf811245bfee4ea093aec64df2819f48fb45059a96e1693fe9001b293eb scripts/verify_godot_failures.py
+8f7b00c9eddc267e0ccba406ab10b1140402c9925b0de9e8c2dcb765c4e634cc tests/test_api.py
+aafe0381b788b72b206eea16011b803acf0ac5b648c1a620760dc8f008c0b2b5 tests/test_checkpoint.py
+134f9eca25a2693d9c2a8e3c50ee946f434c253f7fe5b288be43d94d64c2be71 tests/test_cpu.py
+16ec1436ca16842edcc095a249e061ed797c94d644dcf428ee20399fca6bc102 tests/test_cuda_hardware.py
+f12741c1d3bf4b73a5a3562741a3298c33e961ca78a1636593c0edc7279c820c tests/test_cuda_optional.py
+7fae145cad17b304afa1047237235f1374711143a92bfbcb2a018592caf35e17 tests/test_experiment.py
+bc5f1327c030e93625e496d3c46aef4a7792a009a68710ec990190185bbb8ac4 tests/test_external.py
+ba69005a67f102b7b475a6f6644ce2794e2f9125b1f790b14c2ce2d5f1390bae tests/test_fullbrain_ids.py
+d3cabe6917eec93a33d75951463c0a4e3c950228e77e3aa061a63ad4585172b8 tests/test_godot_bridge.py
+f3c11167fbb7c709cfd60aa272b43ad868a4cf910f7ec2a035958be8d42b2e66 tests/test_importers.py
+d8bf87458883c944da14425d0f7ad6bdfa52c43224c7f262def633281e7d3359 tests/test_olfaction.py
+02f5f6ff5d881f5454f2eaf8db4bec42a72050a5c3553379c6490710e924041c tests/test_open_api.py
+5798d3b158383f3eee254a39ea98d88eab7b2b6a7b7d5af3f9aad241cf4ddfda tests/test_projects.py
+18124d006083819aecabb3e09fceb2ea5de64c23349f9a72594515ccb50c796e tests/test_real_model.py
+feac7bbf905809233aa4945807f43338aead5a986652b56798e00003ed6d6dc0 tests/test_reflex_training.py
+64cf3bd0f6f6d8faaae7702c31ffacb9ddcaf0ca133fee5905d2ff3bdfd120a1 tests/test_registry.py
+c0c32fb68ac85511ffc04f0ae14a42f83da6775311aabaf391e04164d39b937f tests/test_sensory.py
+93007acdb15bd2ad381c5a785b2b69b3879072df81469d07667317b97b2cac7b tests/test_session.py
+acbe6ccd889bb800cd84cc2cf95f9d7d7e015f8449192f311e7d008d22949ee7 tests/test_synaptic.py
+70761bdbb7cbd4fdaa82ea85b40494a84fb532d9d6af350449aa33e46219bb1e tests/test_synaptic_cuda_hardware.py
+284e10f5db01293ad85f16d1a815e029880aaf1e3db7479917bcc7cb9ae6eefa tests/test_synaptic_odor_protocol.py
+8e93c466dad65514e9dc0309d6b15764d2b16fc5e8468f15e246c509863fc31d tests/test_voxel_arena.py
diff --git a/docs/voxel-foraging.md b/docs/voxel-foraging.md
new file mode 100644
index 0000000..ab6dc10
--- /dev/null
+++ b/docs/voxel-foraging.md
@@ -0,0 +1,51 @@
+# Full-brain voxel odor experiment
+
+The requested experiment couples the **entire FlyWire graph on an NVIDIA GPU**
+to a small voxel world. A fruit mesh will represent a simplified food-odor
+source. It does not imply banana vision, a complete odor blend, flight mechanics
+or naturally validated food-seeking behavior.
+
+## Implemented environment
+
+`flybrain.experimental.voxel_arena.VoxelArena` provides floor motion, solid cubic
+obstacles, collision detection, two antenna concentration samples, food-contact
+scoring and JSON world-state replay. It is a kinematic ground body in arbitrary
+game units. Odor uses a Gaussian field without turbulence or obstacle occlusion.
+
+The control boundary is explicit:
+
+1. The arena exposes only `odor_left` and `odor_right` to the sensory adapter.
+2. The adapter schedules events on annotated ORNs in the full network.
+3. A separately declared readout converts neural activity to speed and turn.
+4. `arena.apply({"speed": ..., "turn": ...}, duration_ms=20)` moves the body.
+5. `arena.scene()` supplies positions/food/obstacles to rendering and evaluation;
+ it must not be passed to the neural controller or readout.
+
+Tests verify that relocating food changes the sensed odor, but cannot change
+the trajectory under identical commands. Zero movement commands keep the body
+stationary. Obstacles stop motion, and JSON restore reproduces future pose,
+concentration and collision results.
+
+## Implemented closed loop and remaining behavior work
+
+The [full-graph odor controls](validation/flywire-synaptic-odor-a16.md) establish
+that sensory-only input reaches downstream populations. DNa02 is a candidate steering
+readout based on [descending control experiments](https://www.nature.com/articles/s41586-024-07523-9),
+but this does not establish an appropriate forward-speed mapping, odor response
+or behavior in our graph/dynamics. Any gain, baseline gait, trained readout or
+external reflex must be disclosed in the recording and reproduction recipe.
+
+The [actual GPU recording](validation/flywire-voxel-a16.md) identifies graph,
+configuration, source hashes and GPU. It contains an active and input-silenced
+control with the same initial state and RNG seed, recording body pose, input
+events, neural output and applied commands. Replaying
+it in a browser is **recorded GPU output**, not live browser GPU inference.
+World, brain, delayed events and sensory RNG restore together; the next complete
+frame was replayed exactly in each run. Report
+failures to move or reach food; do not choose successful seeds and hide others.
+
+Run `scripts/run_fullbrain_voxel.py` with the pinned dataset files to produce
+the full-brain recording. `site/foraging.html` renders its saved frames. The active
+run moved, curved left and ended farther from food; the silenced run remained
+stationary. Neither reached food. Calibration and a useful motor readout remain
+unfinished. No successful foraging or training result is claimed.
diff --git a/examples/REFLEX_TRAINING.md b/examples/REFLEX_TRAINING.md
new file mode 100644
index 0000000..77dde9a
--- /dev/null
+++ b/examples/REFLEX_TRAINING.md
@@ -0,0 +1,35 @@
+# Reflex School: train an external action readout
+
+Run from the repository checkout:
+
+```sh
+pip install -e '.[dev]'
+python examples/train_reflex.py --backend cpu --output reflex-training.json
+# On a validated CUDA host, choose --backend cuda instead.
+```
+
+Open the generated `reflex-training.html` to replay the same held-out obstacles
+before and after training. It also writes portable readout weights to
+`reflex-training.checkpoint.json` and full results to JSON. The HTML uses only
+embedded results, needs no server/GPU, and does not perform live inference.
+
+The pinned 313-neuron MaleCNS escape circuit receives left/right looming pulses
+with randomized amplitude and weak opposite-side interference. After 80 ms,
+two GF firing rates and a constant bias become the only controller features.
+The controller chooses left or right. Escaping opposite the obstacle gets reward
+1; moving toward it gets 0. Batched REINFORCE updates three external weights for
+24 rounds using 64 training trials. A separate seed generates 80 balanced test
+trials. Their results never select epochs or update weights. Zero initial weights
+give a 50% deterministic tie-break baseline on this balanced task.
+
+With CUDA selected, both LIF simulation and readout optimization execute through
+CuPy on the GPU. Stimulus generation, reporting and held-out metrics run on CPU.
+This tiny experiment does not imply GPU speedup. All seeds, weights, rates and
+actions are saved so the result can be inspected and reproduced.
+
+**Scientific scope:** the anatomical connectivity stays fixed. The learning is
+an engineered external readout, not biological synaptic plasticity. LIF parameters,
+sensory projections, reward and game rules are designed by us. Trials reset the
+brain and test only a two-choice reflex; this is not a trained whole fruit fly or
+evidence of complex game skill. The animation stretches each saved 80 ms response
+for visibility; it is an illustration of recorded decisions, not physical replay.
diff --git a/examples/godot/bridge.py b/examples/godot/bridge.py
index 65827ea..e32c1b1 100644
--- a/examples/godot/bridge.py
+++ b/examples/godot/bridge.py
@@ -10,8 +10,9 @@
class Bridge:
- def __init__(self, model="toy", download=False):
- demo = make_demo("dodge", model=model, download=download)
+ def __init__(self, model="toy", download=False, *, backend="cpu"):
+ self.backend = backend
+ demo = make_demo("dodge", model=model, download=download, backend=backend)
self.initial = ExternalController(demo.brain, demo.encoder, demo.readout).snapshot()
self.controller = None
self.session_id = None
@@ -19,9 +20,11 @@ def __init__(self, model="toy", download=False):
def dispatch(self, path, data):
if path == "/start":
if "checkpoint_json" in data:
- controller = ExternalController.from_snapshot(json.loads(data["checkpoint_json"]))
+ controller = ExternalController.from_snapshot(
+ json.loads(data["checkpoint_json"]), backend=self.backend
+ )
else:
- controller = ExternalController.from_snapshot(self.initial)
+ controller = ExternalController.from_snapshot(self.initial, backend=self.backend)
if controller.period_ms != 20:
raise ValueError("Godot reference arena requires 20 ms")
self.controller, self.session_id = controller, uuid.uuid4().hex
@@ -44,23 +47,27 @@ def dispatch(self, path, data):
raise ValueError("unknown endpoint")
-def make_server(port=8766, *, model="toy", download=False):
- bridge = Bridge(model, download)
+def make_server(port=8766, *, model="toy", download=False, backend="cpu"):
+ bridge = Bridge(model, download, backend=backend)
class Handler(BaseHTTPRequestHandler):
def do_POST(self):
try:
+ length = int(self.headers.get("Content-Length", "0"))
+ if not 0 < length <= 16_000_000:
+ raise ValueError("request limit: 16 MB")
+ self.connection.settimeout(5)
+ # Consume the bounded body before replying, including rejected
+ # origins. Closing with unread bytes can reset TCP on Windows
+ # and discard the HTTP error response. Never dispatch an origin.
+ body = self.rfile.read(length)
# Browsers receive no cross-origin access to this local dev service.
if (
self.headers.get("Origin")
or self.headers.get_content_type() != "application/json"
):
raise ValueError("native JSON client required")
- length = int(self.headers.get("Content-Length", "0"))
- if not 0 < length <= 16_000_000:
- raise ValueError("request limit: 16 MB")
- self.connection.settimeout(5)
- data = json.loads(self.rfile.read(length))
+ data = json.loads(body)
if not isinstance(data, dict):
raise ValueError("JSON object required")
result = bridge.dispatch(self.path, data)
@@ -87,9 +94,12 @@ def log_message(self, *args):
parser.add_argument("--port", type=int, default=8766)
parser.add_argument("--model", default="toy")
parser.add_argument("--download", action="store_true")
+ parser.add_argument("--backend", choices=("cpu", "cuda"), default="cpu")
args = parser.parse_args()
print("Preparing model before accepting game connections…", flush=True)
- with make_server(args.port, model=args.model, download=args.download) as server:
+ with make_server(
+ args.port, model=args.model, download=args.download, backend=args.backend
+ ) as server:
print(f"Godot bridge: http://127.0.0.1:{server.server_port} · {args.model}", flush=True)
try:
server.serve_forever()
diff --git a/examples/reflex_replay.html b/examples/reflex_replay.html
new file mode 100644
index 0000000..11c314b
--- /dev/null
+++ b/examples/reflex_replay.html
@@ -0,0 +1,24 @@
+
+
+
Reflex School · flybrain-sdk
+
+
+同一回路,学会不同的动作。 来袭在左,就向右闪。来袭在右,就向左闪。固定的 313 神经元 MaleCNS 子回路接收刺激;一个很小的外部动作读出层,通过奖励学习如何解释神经活动。
+训练前 · 留出关卡 —
训练后 · 相同关卡 —
没有参与训练 —
+02 / 奖励训练之后 连接回路不变,仅更新 3 个读出权重。
+暂停回放 重新开始 1 / 80 1× 2× 4×
+
+奖励如何改变动作 绿线:训练集动作准确率 · 蓝线:实际采样奖励。先固定训练轮数,再做一次留出评估。
这个实验说明什么 这是一个简单的两选一反射任务。学到的是外部读出,不是果蝇突触可塑性。关卡之间重置神经状态;留出集改变刺激强度与干扰,尚未证明复杂游戏中的泛化能力。
画面是保存下来的真实实验动作的示意回放。80ms 的神经反应被放慢展示,页面不会连接或租用 GPU,也不在浏览器里重新训练。
+
+
diff --git a/examples/train_reflex.py b/examples/train_reflex.py
new file mode 100644
index 0000000..6ac2be0
--- /dev/null
+++ b/examples/train_reflex.py
@@ -0,0 +1,179 @@
+"""Train an external action readout from a fixed real connectome's firing rates.
+
+One-step contextual bandit: dodge opposite the approaching obstacle (+1), or
+collide (0). This is engineered readout learning, not biological plasticity.
+No labels, cue direction, or reward enter the readout's inference features.
+"""
+
+import argparse
+import hashlib
+import json
+import time
+from pathlib import Path
+
+import numpy as np
+
+from flybrain import FlyBrain
+
+ROOT = Path(__file__).resolve().parents[1]
+IDS = ("10010", "10001")
+
+
+def collect(model, backend, seed, count):
+ rng = np.random.default_rng(seed)
+ sides = np.tile([0, 1], count // 2)
+ rng.shuffle(sides)
+ records = []
+ for index, side in enumerate(sides):
+ brain = FlyBrain.load(model, backend=backend)
+ strength = float(rng.uniform(0.65, 1.0))
+ distractor = float(rng.uniform(0, 0.25))
+ channels = ("looming_left", "looming_right")
+ brain.stimulate(channels[side], strength=strength, duration_ms=80)
+ brain.stimulate(channels[1 - side], strength=distractor, duration_ms=80)
+ brain.advance(duration_ms=80)
+ rates = list(brain.observe(IDS, fields=("rates_hz",)).values["rates_hz"])
+ records.append(
+ {
+ "trial": index,
+ "obstacle_side": int(side),
+ "strength": strength,
+ "distractor": distractor,
+ "rates_hz": rates,
+ "target": int(1 - side),
+ }
+ )
+ return records
+
+
+def features(records):
+ # Rates only; bias permits a learned action preference.
+ rates = np.asarray([r["rates_hz"] for r in records], dtype=np.float64) / 100
+ return np.column_stack([rates, np.ones(len(records))])
+
+
+def probabilities(xp, x, weights):
+ return 1 / (1 + xp.exp(-xp.clip(x @ weights, -30, 30)))
+
+
+def train(x, targets, *, xp=np, epochs=24, seed=23):
+ """REINFORCE with sampled actions and binary reward; fixed 0.5 baseline."""
+ rng = np.random.default_rng(seed)
+ x = xp.asarray(x)
+ targets = xp.asarray(targets)
+ weights = xp.zeros(x.shape[1], dtype=xp.float64)
+ curve = []
+ for epoch in range(epochs):
+ p = probabilities(xp, x, weights)
+ action = (xp.asarray(rng.random(len(x))) < p).astype(xp.int32)
+ reward = (action == targets).astype(xp.float64)
+ weights += 0.8 * x.T @ ((reward - 0.5) * (action - p)) / len(x)
+ greedy = probabilities(xp, x, weights) >= 0.5
+ curve.append(
+ {
+ "epoch": epoch + 1,
+ "sampled_reward": float(reward.mean()),
+ "train_accuracy": float((greedy == targets).mean()),
+ }
+ )
+ return weights, curve
+
+
+def evaluate(records, weights):
+ p = probabilities(np, features(records), np.asarray(weights))
+ trials = [
+ {
+ **r,
+ "probability_right": float(prob),
+ "action": int(prob >= 0.5),
+ "success": int(prob >= 0.5) == r["target"],
+ }
+ for r, prob in zip(records, p)
+ ]
+ return {"accuracy": float(np.mean([t["success"] for t in trials])), "trials": trials}
+
+
+def main():
+ parser = argparse.ArgumentParser(description=__doc__)
+ parser.add_argument("--backend", choices=("cpu", "cuda"), default="cpu")
+ parser.add_argument("--output", type=Path, default=Path("reflex-training.json"))
+ args = parser.parse_args()
+ start = time.perf_counter()
+ brain = FlyBrain.load(ROOT / "models/male-cns-escape-v1/model.json")
+ model = brain.model
+ xp = np
+ if args.backend == "cuda":
+ from flybrain.backends.cuda import _load_cupy
+
+ xp = _load_cupy()
+ training = collect(model, args.backend, 101, 64)
+ # Separate seeds and amplitudes; held-out outcomes never update weights.
+ heldout = collect(model, args.backend, 202, 80)
+ before = evaluate(heldout, [0, 0, 0])
+ weights, curve = train(features(training), [r["target"] for r in training], xp=xp)
+ weights = weights.get().tolist() if args.backend == "cuda" else weights.tolist()
+ after = evaluate(heldout, weights)
+ checkpoint = {
+ "format": "flybrain-external-reflex-readout-v1",
+ "neuron_ids": list(IDS),
+ "scale_hz": 100,
+ "bias_feature": 1,
+ "weights": weights,
+ "model_fingerprint": model.fingerprint,
+ "actions": ["left", "right"],
+ }
+ # Evaluate again after an actual JSON round trip of the portable weights.
+ restored = json.loads(json.dumps(checkpoint))
+ assert evaluate(heldout, restored["weights"]) == after
+ report = {
+ "format": "flybrain-reflex-training-v1",
+ "backend": args.backend,
+ "model": model.name,
+ "neurons": len(model.neuron_ids),
+ "model_fingerprint": model.fingerprint,
+ "checkpoint": checkpoint,
+ "source_sha256": hashlib.sha256(Path(__file__).read_bytes()).hexdigest(),
+ "train_seed": 101,
+ "evaluation_seed": 202,
+ "optimizer_seed": 23,
+ "training_trials": len(training),
+ "evaluation_trials": len(heldout),
+ "epochs": len(curve),
+ "curve": curve,
+ "before": before,
+ "after": after,
+ "seconds": time.perf_counter() - start,
+ "limitations": [
+ "Fixed real anatomical subgraph; LIF parameters and input mapping assumed.",
+ "Only the external three-weight action readout is learned.",
+ "Two-choice 80ms obstacle task; trials reset neural state.",
+ "Held-out trials vary stimulus amplitude, not task or environment.",
+ "Untrained zero weights choose right on ties; chance baseline is 50%.",
+ "No claim of biological learning, whole-fly intelligence or broad generalization.",
+ ],
+ }
+ args.output.parent.mkdir(parents=True, exist_ok=True)
+ args.output.write_text(json.dumps(report, indent=2) + "\n")
+ args.output.with_suffix(".checkpoint.json").write_text(json.dumps(checkpoint, indent=2) + "\n")
+ template = Path(__file__).with_name("reflex_replay.html")
+ if template.exists():
+ args.output.with_suffix(".html").write_text(
+ template.read_text().replace(
+ "__EXPERIMENT_JSON__", json.dumps(report).replace("<", "\\u003c")
+ ),
+ encoding="utf-8",
+ )
+ print(
+ json.dumps(
+ {
+ "before": before["accuracy"],
+ "after": after["accuracy"],
+ "backend": args.backend,
+ "seconds": report["seconds"],
+ }
+ )
+ )
+
+
+if __name__ == "__main__":
+ main()
diff --git a/pyproject.toml b/pyproject.toml
index c2c2cc8..340cd71 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -4,7 +4,7 @@ build-backend = "setuptools.build_meta"
[project]
name = "flybrain-sdk"
-version = "0.4.0a4"
+version = "0.5.0.dev0"
description = "CPU-first connectome simulation for games and experiments. No CUDA required."
readme = "README.md"
requires-python = ">=3.9"
diff --git a/scripts/build_olfactory_map.py b/scripts/build_olfactory_map.py
new file mode 100644
index 0000000..44a9188
--- /dev/null
+++ b/scripts/build_olfactory_map.py
@@ -0,0 +1,122 @@
+"""Resolve pinned FlyWire annotations against the complete official v783 ID set.
+
+No download or inferred hemisphere assignment. The output records exact IDs,
+source checksums and mapping assumptions for downstream CUDA experiments.
+"""
+
+import argparse
+import csv
+import hashlib
+import json
+from pathlib import Path
+
+import numpy as np
+
+ANNOTATION_COMMIT = "8587524c1748ce5ef2080822a2fc890fc03bf597"
+ANNOTATION_URL = (
+ "https://raw.githubusercontent.com/flyconnectome/flywire_annotations/"
+ + ANNOTATION_COMMIT
+ + "/supplemental_files/Supplemental_file1_neuron_annotations.tsv"
+)
+ANNOTATION_SHA256 = "9a4f8b2f843196074431ebd7cd883536afa1be86c8a4ce90970441e8be81d1be"
+ROOTS_SHA256 = "7c7b7e818e9232e5ab64793d52ba20574dbe9da3a6509fbc74193b0f259a01be"
+
+
+def sha256(path):
+ return hashlib.sha256(Path(path).read_bytes()).hexdigest()
+
+
+def resolve_populations(rows, neuron_ids):
+ """Keep IDs as strings throughout; reject uncertain/missing bilateral inputs."""
+ known = set(neuron_ids)
+ groups = {f"ORN_DM1_{side}": [] for side in ("left", "right")}
+ groups.update({f"DNa02_{side}": [] for side in ("left", "right")})
+ groups.update({"ALPN": [], "MBON": [], "descending": []})
+ seen = set()
+ for row in rows:
+ neuron_id = row["root_id"]
+ if not neuron_id.isascii() or not neuron_id.isdecimal():
+ raise ValueError("root_id must be an exact decimal string")
+ if neuron_id in seen:
+ raise ValueError("duplicate annotation root_id")
+ seen.add(neuron_id)
+ cell_type, side = row["cell_type"], row["side"]
+ targets = []
+ if cell_type == "ORN_DM1":
+ if row["super_class"] != "sensory" or row["cell_class"] != "olfactory":
+ raise ValueError("ORN_DM1 has conflicting sensory annotation")
+ if side not in ("left", "right"):
+ raise ValueError("ORN_DM1 side is missing/ambiguous; never split arbitrarily")
+ targets.append(f"ORN_DM1_{side}")
+ if cell_type == "DNa02":
+ if row["super_class"] != "descending" or side not in ("left", "right"):
+ raise ValueError("DNa02 has conflicting descending/side annotation")
+ targets.append(f"DNa02_{side}")
+ for column, value in (
+ ("cell_class", "ALPN"),
+ ("cell_class", "MBON"),
+ ("super_class", "descending"),
+ ):
+ if row[column] == value:
+ targets.append(value)
+ if targets and neuron_id not in known:
+ raise ValueError(f"selected annotation references unknown neuron {neuron_id}")
+ for target in targets:
+ groups[target].append(neuron_id)
+ if any(not values for values in groups.values()):
+ raise ValueError("one or more required sensory/readout populations are empty")
+ return {name: sorted(values, key=int) for name, values in groups.items()}
+
+
+def build(annotations, roots):
+ if sha256(annotations) != ANNOTATION_SHA256 or sha256(roots) != ROOTS_SHA256:
+ raise ValueError("pinned source checksum mismatch")
+ ids = np.load(roots, allow_pickle=False)
+ if ids.dtype.kind not in "iu" or len(ids) != 139255 or len(np.unique(ids)) != 139255:
+ raise ValueError("expected all 139255 official integer neuron IDs")
+ with annotations.open(newline="", encoding="utf-8") as f:
+ groups = resolve_populations(csv.DictReader(f, delimiter="\t"), map(str, ids))
+ if len(groups["ORN_DM1_left"]) != 35 or len(groups["ORN_DM1_right"]) != 33:
+ raise ValueError("pinned DM1 population counts changed")
+ return {
+ "schema": "flybrain-olfactory-map-v1",
+ "model": "flywire-full-v783",
+ "neuron_count": 139255,
+ "annotation_source": {
+ "url": ANNOTATION_URL,
+ "commit": ANNOTATION_COMMIT,
+ "sha256": ANNOTATION_SHA256,
+ "license": "not specified in the pinned repository; do not infer SDK MIT coverage",
+ "citation": "Schlegel et al. 2024; Matsliah et al. 2024; Berg et al. 2025/2026",
+ },
+ "root_ids_sha256": ROOTS_SHA256,
+ "stimulus": {
+ "label": "ethyl-acetate / Or42b channel approximation",
+ "receptor": "Or42b",
+ "glomerulus": "DM1",
+ "evidence": "https://www.nature.com/articles/s41598-017-13015-w",
+ "scope": (
+ "One responsive receptor channel; not the complete ethyl-acetate or banana response"
+ ),
+ "current_units": "dimensionless; not measured current or calibrated odor concentration",
+ "hemispheres": "source annotation side; no invented left/right assignments",
+ },
+ "groups": groups,
+ "counts": {name: len(values) for name, values in groups.items()},
+ }
+
+
+def main():
+ parser = argparse.ArgumentParser(description=__doc__)
+ parser.add_argument("--annotations", type=Path, required=True)
+ parser.add_argument("--roots", type=Path, required=True)
+ parser.add_argument("--output", type=Path, required=True)
+ args = parser.parse_args()
+ report = build(args.annotations, args.roots)
+ args.output.parent.mkdir(parents=True, exist_ok=True)
+ args.output.write_text(json.dumps(report, indent=2) + "\n", encoding="utf-8")
+ print(json.dumps(report["counts"]))
+
+
+if __name__ == "__main__":
+ main()
diff --git a/scripts/probe_fullbrain_odor.py b/scripts/probe_fullbrain_odor.py
new file mode 100644
index 0000000..e0dd6f3
--- /dev/null
+++ b/scripts/probe_fullbrain_odor.py
@@ -0,0 +1,166 @@
+"""CUDA-only full FlyWire sensory propagation experiment, not a behavior claim.
+
+Five matched conditions start from exactly the same saved resting state. No
+global background current, random input, decoder training or graph pruning.
+Reports every condition, including absent downstream responses. Source data and
+annotations must already exist locally; see docs/olfactory-experiment.md.
+"""
+
+import argparse
+import json
+import time
+from pathlib import Path
+
+import numpy as np
+from build_olfactory_map import build
+from validate_fullbrain import digest, load_fullbrain
+
+from flybrain.backends.cuda import CUDABackend
+from flybrain.config import LIFConfig
+from flybrain.neurons import NeuronIndex
+from flybrain.olfaction import OlfactoryDrive
+
+CONDITIONS = (
+ ("no_odor", 0.0, 0.0, False),
+ ("left_odor", 1.0, 0.0, False),
+ ("right_odor", 0.0, 1.0, False),
+ ("bilateral_odor", 1.0, 1.0, False),
+ ("bilateral_odor_ORNs_silenced", 1.0, 1.0, True),
+)
+
+
+def main():
+ parser = argparse.ArgumentParser(description=__doc__)
+ parser.add_argument("--data-dir", type=Path, required=True)
+ parser.add_argument("--annotations", type=Path, required=True)
+ parser.add_argument("--output", type=Path, required=True)
+ args = parser.parse_args()
+ report = {
+ "schema": "flybrain-full-odor-probe-v1",
+ "status": "running",
+ "started_utc": time.strftime("%Y-%m-%dT%H:%M:%SZ", time.gmtime()),
+ "script_sha256": digest(__file__),
+ "config": LIFConfig().to_dict(),
+ "protocol": {
+ "pre_ms": 100,
+ "odor_ms": 500,
+ "post_ms": 200,
+ "trace_interval_ms": 10,
+ "stimulus": "Or42b/DM1 single-channel food-odor approximation",
+ "max_current": 5.0,
+ "half_concentration": 0.2,
+ "background_current": 0.0,
+ "weight_policy": "unchanged full-brain benchmark",
+ "concentration_units": "dimensionless, not a measured chemical dose",
+ },
+ "conditions": [],
+ }
+ args.output.parent.mkdir(parents=True, exist_ok=True)
+
+ def save():
+ args.output.write_text(json.dumps(report, indent=2, allow_nan=False) + "\n")
+
+ save()
+ try:
+ started = time.perf_counter()
+ mapping = build(args.annotations, args.data_dir / "proofread_root_ids_783.npy")
+ model, report["model"] = load_fullbrain(args.data_dir)
+ report["mapping"] = mapping
+ index = NeuronIndex(model)
+ groups = {name: index.resolve(ids) for name, ids in mapping["groups"].items()}
+ selected = tuple(sorted({i for values in groups.values() for i in values}))
+ selected_index = {i: j for j, i in enumerate(selected)}
+ positions = {k: [selected_index[i] for i in v] for k, v in groups.items()}
+ brain = CUDABackend(model, LIFConfig()) # No CPU fallback permitted.
+ brain._stream.synchronize()
+ cp = brain._cp
+ props = cp.cuda.runtime.getDeviceProperties(cp.cuda.Device().id)
+ report["device"] = {
+ "name": props["name"].decode(),
+ "memory_bytes": props["totalGlobalMem"],
+ "cupy": cp.__version__,
+ "driver": cp.cuda.runtime.driverGetVersion(),
+ "runtime": cp.cuda.runtime.runtimeGetVersion(),
+ }
+ report["load_seconds"] = time.perf_counter() - started
+ report["cuda_kernel_sha256"] = digest(
+ Path(__file__).parents[1] / "src/flybrain/backends/cuda.py"
+ )
+ odor = OlfactoryDrive(
+ model,
+ left_ids=mapping["groups"]["ORN_DM1_left"],
+ right_ids=mapping["groups"]["ORN_DM1_right"],
+ )
+ zero = np.zeros(len(model.neuron_ids))
+ # Same graph + backend state, through JSON, as in the full benchmark.
+ rest = json.loads(json.dumps(brain.snapshot(), allow_nan=False))
+ for name, left, right, silence in CONDITIONS:
+ brain.restore(rest)
+ if silence:
+ brain.set_silenced(groups["ORN_DM1_left"] + groups["ORN_DM1_right"], True)
+ current = odor.current(left=left, right=right)
+ item = {
+ "name": name,
+ "antenna_concentrations": [left, right],
+ "silence_input_neurons": silence,
+ "spikes": {group: 0 for group in groups},
+ "trace": [],
+ }
+ started = time.perf_counter()
+ spike_totals = np.zeros(len(selected), dtype=np.int64)
+ for tick in range(800):
+ brain.step(current if 100 <= tick < 600 else zero)
+ observed = brain.observe_selected(selected, ("spikes",))
+ spike_totals += np.asarray(observed["spikes"], dtype=np.int64)
+ if (tick + 1) % 10 == 0:
+ observed = brain.observe_selected(selected, ("voltage", "rates_hz"))
+ voltage = np.asarray(observed["voltage"])
+ rates = np.asarray(observed["rates_hz"])
+ if not np.isfinite(voltage).all() or not np.isfinite(rates).all():
+ raise ValueError("nonfinite neural response")
+ item["trace"].append(
+ {
+ "time_ms": tick + 1,
+ "groups": {
+ group: {
+ "mean_rate_hz": float(rates[pos].mean()),
+ "max_voltage": float(voltage[pos].max()),
+ }
+ for group, pos in positions.items()
+ },
+ }
+ )
+ item["spikes"] = {k: int(spike_totals[p].sum()) for k, p in positions.items()}
+ item["wall_seconds"] = time.perf_counter() - started
+ report["conditions"].append(item)
+ save()
+ print(json.dumps({k: v for k, v in item.items() if k != "trace"}), flush=True)
+ conditions = {item["name"]: item for item in report["conditions"]}
+ for name in ("no_odor", "bilateral_odor_ORNs_silenced"):
+ if any(conditions[name]["spikes"].values()):
+ raise AssertionError("unexpected spikes in matched no-input control")
+ for side in ("left", "right"):
+ counts = conditions[f"{side}_odor"]["spikes"]
+ if counts[f"ORN_DM1_{side}"] == 0:
+ raise AssertionError("input stimulation failed to produce ORN spikes")
+ report["findings"] = {
+ "input_stimulation_and_silencing_checks": "passed",
+ "bilateral_descending_spikes": conditions["bilateral_odor"]["spikes"]["descending"],
+ "interpretation": (
+ "Presence of descending spikes does not prove odor-directed navigation; "
+ "absence means this preset has not demonstrated the required motor response. "
+ "No behavior, banana recognition, flight or learning was tested here."
+ ),
+ }
+ report["status"] = "completed"
+ except Exception as error:
+ report["status"] = "failed"
+ report["error"] = f"{type(error).__name__}: {error}"
+ raise
+ finally:
+ report["finished_utc"] = time.strftime("%Y-%m-%dT%H:%M:%SZ", time.gmtime())
+ save()
+
+
+if __name__ == "__main__":
+ main()
diff --git a/scripts/probe_synaptic_odor.py b/scripts/probe_synaptic_odor.py
new file mode 100644
index 0000000..1d24748
--- /dev/null
+++ b/scripts/probe_synaptic_odor.py
@@ -0,0 +1,254 @@
+"""Full FlyWire CUDA odor experiment with separately versioned mV dynamics.
+
+Keeps every official neuron/edge. Seeded discrete Poisson inputs target only
+annotated DM1 ORNs. No CPU fallback, background current, navigation controller,
+parameter search, graph pruning or success-based selection of conditions.
+"""
+
+import argparse
+import json
+import time
+from pathlib import Path
+
+import numpy as np
+from build_olfactory_map import build
+from validate_fullbrain import digest, load_fullbrain
+
+from flybrain.experimental.synaptic import SynapticCPU, SynapticLIFConfig
+from flybrain.experimental.synaptic_cuda import SynapticCUDA
+from flybrain.neurons import NeuronIndex
+
+CONDITIONS = (
+ ("no_odor", False, False, False),
+ ("left_odor", True, False, False),
+ ("right_odor", False, True, False),
+ ("bilateral_odor", True, True, False),
+ ("bilateral_odor_ORNs_silenced", True, True, True),
+)
+
+
+def input_events(rng, left_count, right_count, probability, left, right):
+ """Matched random draws even when a side is disabled; one event/cell/tick.
+
+ Bernoulli p=rate*dt is the discrete PoissonGroup approximation. It is not
+ an exact continuous-time Poisson process or a calibrated chemical dose.
+ """
+ events = rng.random(left_count + right_count) < probability
+ if not left:
+ events[:left_count] = False
+ if not right:
+ events[left_count:] = False
+ return events
+
+
+def compare(cpu, gpu):
+ expected, actual = cpu.snapshot(), gpu.snapshot()
+ for field in (
+ "engine",
+ "fingerprint",
+ "tick",
+ "spikes",
+ "history",
+ "silenced",
+ "last_spike_tick",
+ ):
+ if expected[field] != actual[field]:
+ raise AssertionError(f"CPU/GPU mismatch: {field}")
+ errors = {}
+ for field in ("voltage_mv", "synaptic_mv", "rates_hz"):
+ a, b = np.asarray(expected[field]), np.asarray(actual[field])
+ np.testing.assert_allclose(a, b, atol=1e-9, rtol=0, err_msg=field)
+ errors[field] = float(np.max(np.abs(a - b)))
+ return errors
+
+
+def main():
+ parser = argparse.ArgumentParser(description=__doc__)
+ parser.add_argument("--data-dir", type=Path, required=True)
+ parser.add_argument("--annotations", type=Path, required=True)
+ parser.add_argument("--output", type=Path, required=True)
+ args = parser.parse_args()
+ config = SynapticLIFConfig()
+ seed, rate_hz, jump_mv, contact_mv = 20260913, 150.0, 68.75, 0.275
+ probability = rate_hz * config.dt_ms / 1000
+ report = {
+ "schema": "flybrain-full-synaptic-odor-v1",
+ "status": "running",
+ "started_utc": time.strftime("%Y-%m-%dT%H:%M:%SZ", time.gmtime()),
+ "config": config.to_dict(),
+ "numpy": np.__version__,
+ "source_sha256": {
+ str(p.relative_to(Path(__file__).resolve().parents[1])): digest(p)
+ for p in (
+ Path(__file__).resolve(),
+ Path(__file__).with_name("validate_fullbrain.py").resolve(),
+ Path(__file__).with_name("build_olfactory_map.py").resolve(),
+ *sorted(
+ (Path(__file__).resolve().parents[1] / "src/flybrain/experimental").glob("*.py")
+ ),
+ )
+ },
+ "protocol": {
+ "pre_ms": 100,
+ "odor_ms": 500,
+ "post_ms": 200,
+ "trace_interval_ms": 10,
+ "seed": seed,
+ "rng": "NumPy PCG64",
+ "input_rate_hz": rate_hz,
+ "input_jump_mv": jump_mv,
+ "contact_mv": contact_mv,
+ "background_input": 0,
+ "sensory_model": "Bernoulli p=rate*dt, at most one input event/cell/tick",
+ "stimulus": "Or42b/DM1 single-channel food-odor approximation",
+ "calibration": "nominal research parameters; not a measured banana dose",
+ "input_refractory_ms": 0,
+ "parameter_source": (
+ "https://github.com/philshiu/Drosophila_brain_model/"
+ "blob/91bdd1e7dcf193f3e7ca5a8933497fcef63b7960/model.py"
+ ),
+ },
+ "conditions": [],
+ }
+ args.output.parent.mkdir(parents=True, exist_ok=True)
+
+ def save():
+ temporary = args.output.with_suffix(".partial.json")
+ temporary.write_text(json.dumps(report, indent=2, allow_nan=False) + "\n")
+ temporary.replace(args.output)
+
+ save()
+ try:
+ mapping = build(args.annotations, args.data_dir / "proofread_root_ids_783.npy")
+ model, report["model"] = load_fullbrain(args.data_dir, contact_mv=contact_mv)
+ report["mapping"] = mapping
+ index = NeuronIndex(model)
+ groups = {name: index.resolve(ids) for name, ids in mapping["groups"].items()}
+ left_ids, right_ids = (mapping["groups"][f"ORN_DM1_{side}"] for side in ("left", "right"))
+ input_ids = left_ids + right_ids
+ inputs = np.asarray(index.resolve(input_ids), dtype=np.int64)
+ selected = tuple(sorted({i for values in groups.values() for i in values}))
+ offsets = {i: j for j, i in enumerate(selected)}
+ positions = {name: [offsets[i] for i in values] for name, values in groups.items()}
+ brain = SynapticCUDA(model, config, input_ids=input_ids, weight_units="mV")
+ props = brain._cp.cuda.runtime.getDeviceProperties(brain._device.id)
+ report["device"] = {
+ "name": props["name"].decode(),
+ "memory_bytes": props["totalGlobalMem"],
+ "cupy": brain._cp.__version__,
+ "driver": brain._cp.cuda.runtime.driverGetVersion(),
+ "runtime": brain._cp.cuda.runtime.runtimeGetVersion(),
+ }
+ rest = json.loads(json.dumps(brain.snapshot(), allow_nan=False))
+ jumps = np.zeros(brain.n)
+ cpu = SynapticCPU(model, config, input_ids=input_ids, weight_units="mV")
+ rng = np.random.default_rng(seed)
+ parity = {"ticks": 200, "observations": [], "input_events": 0}
+ started = time.perf_counter()
+ for tick in range(200):
+ events = input_events(rng, len(left_ids), len(right_ids), probability, True, True)
+ jumps[inputs] = events * jump_mv
+ parity["input_events"] += int(events.sum())
+ cpu.step(jumps)
+ brain.step(jumps)
+ if (tick + 1) % 40 == 0:
+ parity["observations"].append({"tick": tick + 1, "errors": compare(cpu, brain)})
+ if parity["input_events"] == 0:
+ raise AssertionError("full-graph parity must exercise active sensory input")
+ # Round-trip device state through JSON and replay the same input RNG.
+ checkpoint = json.loads(json.dumps(brain.snapshot(), allow_nan=False))
+ rng_state = json.loads(json.dumps(rng.bit_generator.state))
+
+ def replay():
+ for _ in range(20):
+ events = input_events(rng, len(left_ids), len(right_ids), probability, True, True)
+ jumps[inputs] = events * jump_mv
+ brain.step(jumps)
+ return brain.snapshot()
+
+ expected = replay()
+ brain.restore(checkpoint)
+ rng.bit_generator.state = rng_state
+ if replay() != expected:
+ raise AssertionError("full-graph device + input RNG checkpoint replay differed")
+ parity.update(
+ status="passed",
+ json_checkpoint_replay_ticks=20,
+ wall_seconds=time.perf_counter() - started,
+ )
+ report["full_graph_cpu_cuda_parity"] = parity
+ save()
+ print("Full-graph active parity and delayed checkpoint replay passed", flush=True)
+ del cpu, checkpoint, expected
+ for name, left, right, silence in CONDITIONS:
+ brain.restore(rest)
+ if silence:
+ brain.set_silenced(inputs, True)
+ rng = np.random.default_rng(seed)
+ totals = np.zeros(len(selected), dtype=np.int64)
+ item = {"name": name, "silenced": silence, "input_events": [0, 0], "trace": []}
+ started = time.perf_counter()
+ for tick in range(8000):
+ active = 1000 <= tick < 6000
+ events = input_events(
+ rng,
+ len(left_ids),
+ len(right_ids),
+ probability,
+ left and active,
+ right and active,
+ )
+ jumps[inputs] = events * jump_mv
+ item["input_events"][0] += int(events[: len(left_ids)].sum())
+ item["input_events"][1] += int(events[len(left_ids) :].sum())
+ brain.step(jumps)
+ totals += np.asarray(
+ brain.observe_selected(selected, ("spikes",))["spikes"], dtype=np.int64
+ )
+ if (tick + 1) % 100 == 0:
+ observed = brain.observe_selected(selected, ("voltage_mv", "rates_hz"))
+ voltage, rates = (np.asarray(observed[k]) for k in ("voltage_mv", "rates_hz"))
+ item["trace"].append(
+ {
+ "time_ms": (tick + 1) * config.dt_ms,
+ "groups": {
+ group: {
+ "mean_rate_hz": float(rates[pos].mean()),
+ "max_voltage_mv": float(voltage[pos].max()),
+ "cumulative_spikes": int(totals[pos].sum()),
+ }
+ for group, pos in positions.items()
+ },
+ }
+ )
+ item["spikes"] = {group: int(totals[pos].sum()) for group, pos in positions.items()}
+ item["wall_seconds"] = time.perf_counter() - started
+ report["conditions"].append(item)
+ save()
+ print(json.dumps({k: v for k, v in item.items() if k != "trace"}), flush=True)
+ conditions = {item["name"]: item for item in report["conditions"]}
+ for name in ("no_odor", "bilateral_odor_ORNs_silenced"):
+ if any(conditions[name]["spikes"].values()):
+ raise AssertionError("spikes in matched no-input control")
+ for side in ("left", "right"):
+ if conditions[f"{side}_odor"]["spikes"][f"ORN_DM1_{side}"] == 0:
+ raise AssertionError("stimulated ORNs did not spike")
+ report["findings"] = {
+ "input_and_silencing_controls": "passed",
+ "bilateral_descending_spikes": conditions["bilateral_odor"]["spikes"]["descending"],
+ "interpretation": (
+ "Report includes absent responses. Descending spikes alone do not prove "
+ "odor-directed navigation. No banana recognition, flight or learning tested."
+ ),
+ }
+ report["status"] = "completed"
+ except Exception as error:
+ report.update(status="failed", error=f"{type(error).__name__}: {error}")
+ raise
+ finally:
+ report["finished_utc"] = time.strftime("%Y-%m-%dT%H:%M:%SZ", time.gmtime())
+ save()
+
+
+if __name__ == "__main__":
+ main()
diff --git a/scripts/run_fullbrain_voxel.py b/scripts/run_fullbrain_voxel.py
new file mode 100644
index 0000000..b0545ab
--- /dev/null
+++ b/scripts/run_fullbrain_voxel.py
@@ -0,0 +1,214 @@
+"""Record a complete FlyWire CUDA brain controlling a ground voxel body.
+
+Experimental engineering readout: DNa02 rate difference controls turn; summed
+rates control speed. The speed mapping is not biologically established. No
+target coordinate, odor concentration or rescue reflex enters the motor readout.
+Both active and input-silenced runs are retained, regardless of food contact.
+"""
+
+import argparse
+import json
+import math
+import time
+from pathlib import Path
+
+import numpy as np
+from build_olfactory_map import build
+from validate_fullbrain import digest, load_fullbrain
+
+from flybrain.experimental.synaptic import SynapticLIFConfig
+from flybrain.experimental.synaptic_cuda import SynapticCUDA
+from flybrain.experimental.voxel_arena import VoxelArena
+from flybrain.neurons import NeuronIndex
+
+
+def motor_readout(left_hz, right_hz):
+ """Fixed, untrained engineering gains. Inputs are neural firing rates only."""
+ return {
+ "speed": min(3.0, max(0.0, (left_hz + right_hz) * 0.03)),
+ "turn": 2.0 * math.tanh((right_hz - left_hz) / 40.0),
+ }
+
+
+def main():
+ parser = argparse.ArgumentParser(description=__doc__)
+ parser.add_argument("--data-dir", type=Path, required=True)
+ parser.add_argument("--annotations", type=Path, required=True)
+ parser.add_argument("--output", type=Path, required=True)
+ args = parser.parse_args()
+ root = Path(__file__).resolve().parents[1]
+ config, seed = SynapticLIFConfig(), 20260913
+ report = {
+ "schema": "flybrain-voxel-recording-v1",
+ "status": "running",
+ "started_utc": time.strftime("%Y-%m-%dT%H:%M:%SZ", time.gmtime()),
+ "config": config.to_dict(),
+ "seed": seed,
+ "source_sha256": {
+ str(p.relative_to(root)): digest(p)
+ for p in (
+ Path(__file__).resolve(),
+ root / "scripts/validate_fullbrain.py",
+ root / "scripts/build_olfactory_map.py",
+ root / "src/flybrain/olfaction.py",
+ *sorted((root / "src/flybrain/experimental").glob("*.py")),
+ )
+ },
+ "protocol": {
+ "duration_ms": 2000,
+ "body_step_ms": 20,
+ "neural_steps_per_frame": 200,
+ "input_jump_mv": 68.75,
+ "contact_mv": 0.275,
+ "odor_rate_hz": "180*c/(0.2+c); c is a dimensionless antenna sample",
+ "stimulus": "DM1 single-channel food odor, not a full banana mixture",
+ "readout": "speed=clip(0.03*(DNa02_L+DNa02_R),0,3); turn=2*tanh((DNa02_R-DNa02_L)/40)",
+ "units": "rates Hz; speed game units/s; turn radians/s",
+ "limitations": [
+ "Untrained engineering readout; forward-speed mapping not established biologically",
+ "Kinematic walking body, not flight or musculoskeletal simulation",
+ "Toy Gaussian odor field without wind, turbulence or obstacle occlusion",
+ "Recorded GPU frames, not live browser inference",
+ "No direct motor input, background drive, odor-bearing controller or rescue reflex",
+ "Success or failure of both runs reported without seed selection",
+ ],
+ },
+ "runs": [],
+ }
+ args.output.parent.mkdir(parents=True, exist_ok=True)
+
+ def save():
+ temp = args.output.with_suffix(".partial.json")
+ temp.write_text(json.dumps(report, indent=2, allow_nan=False) + "\n")
+ temp.replace(args.output)
+
+ save()
+ try:
+ mapping = build(args.annotations, args.data_dir / "proofread_root_ids_783.npy")
+ model, report["model"] = load_fullbrain(args.data_dir, contact_mv=0.275)
+ report["mapping"] = mapping
+ # The map's historical dimensionless-current label does not configure this engine.
+ report["mapping_input_units_note"] = "Actual input units and strengths are in protocol."
+ index = NeuronIndex(model)
+ left_ids, right_ids = (mapping["groups"][f"ORN_DM1_{s}"] for s in ("left", "right"))
+ input_ids = left_ids + right_ids
+ inputs = np.asarray(index.resolve(input_ids), dtype=np.int64)
+ outputs = index.resolve(mapping["groups"]["DNa02_left"] + mapping["groups"]["DNa02_right"])
+ brain = SynapticCUDA(model, config, input_ids=input_ids, weight_units="mV")
+ props = brain._cp.cuda.runtime.getDeviceProperties(brain._device.id)
+ report["device"] = {
+ "name": props["name"].decode(),
+ "memory_bytes": props["totalGlobalMem"],
+ "cupy": brain._cp.__version__,
+ "driver": brain._cp.cuda.runtime.driverGetVersion(),
+ "runtime": brain._cp.cuda.runtime.runtimeGetVersion(),
+ }
+ rest = json.loads(json.dumps(brain.snapshot(), allow_nan=False))
+ jumps = np.zeros(brain.n)
+ for silence in (False, True):
+ world = VoxelArena(food=(-1.6, 0.5, -0.6))
+ brain.restore(rest)
+ brain.set_silenced(inputs, silence)
+ rng = np.random.default_rng(seed)
+ run = {
+ "name": "ORNs_silenced" if silence else "neural_control",
+ "initial": world.scene(),
+ "frames": [],
+ }
+ started = time.perf_counter()
+
+ def advance_frame():
+ odor = world.observe()
+ rates = np.asarray(
+ [
+ 180 * odor[f"odor_{side}"] / (0.2 + odor[f"odor_{side}"])
+ for side in ("left", "right")
+ ]
+ )
+ probability = np.repeat(rates, [len(left_ids), len(right_ids)]) * 0.0001
+ input_count = np.zeros(2, dtype=np.int64)
+ output_count = np.zeros(2, dtype=np.int64)
+ for _ in range(200):
+ events = rng.random(len(inputs)) < probability
+ jumps[inputs] = events * 68.75
+ input_count += [
+ int(events[: len(left_ids)].sum()),
+ int(events[len(left_ids) :].sum()),
+ ]
+ brain.step(jumps)
+ output_count += np.asarray(
+ brain.observe_selected(outputs, ("spikes",))["spikes"], dtype=np.int64
+ )
+ neural = brain.observe_selected(outputs, ("rates_hz",))["rates_hz"]
+ action = motor_readout(*neural)
+ world.apply(action, 20)
+ return {
+ **world.scene(),
+ "odor": odor,
+ "input_rate_hz": rates.tolist(),
+ "input_events": input_count.tolist(),
+ "output_spikes": output_count.tolist(),
+ "output_rates_hz": list(neural),
+ "action": action,
+ }
+
+ for frame in range(100):
+ record = advance_frame()
+ run["frames"].append(record)
+ if frame == 19:
+ # A saved body state plus brain delay buffers and sensory RNG,
+ # restored before the next frame. No hidden renderer state.
+ saved_brain = json.loads(json.dumps(brain.snapshot(), allow_nan=False))
+ saved_world = json.loads(json.dumps(world.snapshot(), allow_nan=False))
+ saved_rng = json.loads(json.dumps(rng.bit_generator.state))
+ expected_frame = advance_frame()
+ expected_brain = brain.snapshot()
+ expected_rng = rng.bit_generator.state
+ brain.restore(saved_brain)
+ world = VoxelArena.from_snapshot(saved_world)
+ rng.bit_generator.state = saved_rng
+ if (
+ advance_frame() != expected_frame
+ or brain.snapshot() != expected_brain
+ or rng.bit_generator.state != expected_rng
+ ):
+ raise AssertionError("joint brain/world/RNG replay differed")
+ brain.restore(saved_brain)
+ world = VoxelArena.from_snapshot(saved_world)
+ rng.bit_generator.state = saved_rng
+ run["checkpoint_replay"] = {
+ "at_frame": 20,
+ "future_frames": 1,
+ "full_brain_world_and_rng_exact": True,
+ }
+ if (frame + 1) % 20 == 0:
+ print(
+ json.dumps(
+ {
+ "run": run["name"],
+ "frame": frame + 1,
+ "position": world.scene()["position"],
+ "rates_hz": record["output_rates_hz"],
+ }
+ ),
+ flush=True,
+ )
+ run["wall_seconds"] = time.perf_counter() - started
+ run["final_distance"] = math.dist(world.scene()["position"], world.food)
+ run["initial_distance"] = math.dist(run["initial"]["position"], world.food)
+ run["reached_food"] = world.reached_food
+ if silence and world.scene()["position"] != run["initial"]["position"]:
+ raise AssertionError("silenced control moved unexpectedly")
+ report["runs"].append(run)
+ save()
+ report["status"] = "completed"
+ except Exception as error:
+ report.update(status="failed", error=f"{type(error).__name__}: {error}")
+ raise
+ finally:
+ report["finished_utc"] = time.strftime("%Y-%m-%dT%H:%M:%SZ", time.gmtime())
+ save()
+
+
+if __name__ == "__main__":
+ main()
diff --git a/scripts/validate_cuda.py b/scripts/validate_cuda.py
new file mode 100644
index 0000000..6a3973e
--- /dev/null
+++ b/scripts/validate_cuda.py
@@ -0,0 +1,142 @@
+"""Actual-device conformance gate and small-model latency report; never an emulator."""
+
+import argparse
+import hashlib
+import json
+import os
+import platform
+import statistics
+import subprocess
+import sys
+import tempfile
+import time
+import xml.etree.ElementTree as ET
+from datetime import datetime, timezone
+from pathlib import Path
+
+ROOT = Path(__file__).resolve().parents[1]
+
+
+def validate(report):
+ import numpy as np
+
+ from flybrain import FlyBrain
+ from flybrain.backends.cuda import _load_cupy
+
+ cp = _load_cupy()
+ device = cp.cuda.runtime.getDeviceProperties(cp.cuda.Device().id)
+ name = device["name"]
+ report["environment"] = {
+ "python": platform.python_version(),
+ "platform": platform.platform(),
+ "numpy": np.__version__,
+ "cupy": cp.__version__,
+ "device": name.decode() if isinstance(name, bytes) else name,
+ "compute_capability": [device["major"], device["minor"]],
+ "device_memory_bytes": device["totalGlobalMem"],
+ "cuda_driver": cp.cuda.runtime.driverGetVersion(),
+ "cuda_runtime": cp.cuda.runtime.runtimeGetVersion(),
+ }
+ # A positive device count is not a conformance pass. Require every hardware
+ # test to execute successfully; a skipped test fails this gate.
+ with tempfile.TemporaryDirectory() as folder:
+ results = Path(folder) / "pytest.xml"
+ subprocess.run(
+ [
+ sys.executable,
+ "-m",
+ "pytest",
+ "tests/test_cuda_hardware.py",
+ "-q",
+ f"--junitxml={results}",
+ ],
+ cwd=ROOT,
+ env={**os.environ, "FLYBRAIN_REQUIRE_CUDA": "1"},
+ check=True,
+ timeout=300,
+ )
+ suites = list(ET.parse(results).getroot().iter("testsuite"))
+ counts = {
+ k: sum(int(s.get(k, 0)) for s in suites)
+ for k in ("tests", "failures", "errors", "skipped")
+ }
+ if counts["tests"] < 12 or any(counts[k] for k in ("failures", "errors", "skipped")):
+ raise RuntimeError(f"Incomplete CUDA validation: {counts}")
+ report["conformance"] = counts
+ report["benchmarks"] = []
+ for model in ("toy", ROOT / "models/male-cns-escape-v1/model.json"):
+ loaded = FlyBrain.load(model)
+ measurements = {}
+ for backend in ("cpu", "cuda"):
+ cold_start = time.perf_counter()
+ brain = FlyBrain.load(model, backend=backend)
+ measurements[backend + "_load_seconds"] = time.perf_counter() - cold_start
+ brain.stimulate(brain.sensory_channels[0], duration_ms=4000)
+ brain.advance(duration_ms=100)
+ times = []
+ for _ in range(3):
+ start = time.perf_counter()
+ brain.advance(duration_ms=1000)
+ brain.action()
+ if backend == "cuda":
+ cp.cuda.get_current_stream().synchronize()
+ times.append(time.perf_counter() - start)
+ measurements[backend] = {"seconds": times, "median_seconds": statistics.median(times)}
+ report["benchmarks"].append(
+ {
+ "model": loaded.model.name,
+ "fingerprint": loaded.model.fingerprint,
+ "neurons": len(loaded.model.neuron_ids),
+ "edges": len(loaded.model.synapses),
+ "config": loaded.config.to_dict(),
+ "simulated_ms_per_repeat": 1000,
+ "warmup_ms": 100,
+ "timings": measurements,
+ }
+ )
+ report["limitations"] = [
+ "Correctness-first CUDA: host current upload and scalar synchronization each tick.",
+ "These timings cover only toy and 313-cell circuit, not whole-brain scaling.",
+ "Loading/compilation reported separately; execution includes inputs and action readout.",
+ "Conformance applies to tested cases and reported device/software, not all CUDA hardware.",
+ ]
+ report["status"] = "passed"
+
+
+def main():
+ parser = argparse.ArgumentParser(description=__doc__)
+ parser.add_argument("--output", type=Path, required=True)
+ args = parser.parse_args()
+ report = {
+ "format": "flybrain-cuda-validation",
+ "schema_version": 1,
+ "status": "failed",
+ "started_at": datetime.now(timezone.utc).isoformat(),
+ "source_sha256": {
+ str(p.relative_to(ROOT)): hashlib.sha256(p.read_bytes()).hexdigest()
+ for p in sorted(
+ list((ROOT / "src/flybrain").rglob("*.py"))
+ + [ROOT / "tests/test_cuda_hardware.py", Path(__file__).resolve()]
+ )
+ },
+ }
+ code = 0
+ try:
+ validate(report)
+ except Exception as error:
+ report["error"] = f"{type(error).__name__}: {error}"
+ code = 1
+ report["finished_at"] = datetime.now(timezone.utc).isoformat()
+ args.output.parent.mkdir(parents=True, exist_ok=True)
+ args.output.write_text(json.dumps(report, indent=2) + "\n", encoding="utf-8")
+ print(
+ json.dumps(
+ {"status": report["status"], "output": str(args.output), "error": report.get("error")},
+ indent=2,
+ )
+ )
+ return code
+
+
+if __name__ == "__main__":
+ raise SystemExit(main())
diff --git a/scripts/validate_cuda_modal.py b/scripts/validate_cuda_modal.py
new file mode 100644
index 0000000..dbb6977
--- /dev/null
+++ b/scripts/validate_cuda_modal.py
@@ -0,0 +1,59 @@
+"""Optional, manually invoked Modal T4 validation. Running this can incur charges.
+
+Only the source package, one test file, validator and small checked-in model are
+uploaded. No home directory, credential files, raw datasets or .git are included.
+"""
+
+import json
+from pathlib import Path
+
+import modal
+
+ROOT = Path(__file__).resolve().parents[1]
+image = (
+ modal.Image.debian_slim(python_version="3.12")
+ .pip_install("numpy==2.2.6", "pytest==8.4.2", "cupy-cuda12x[ctk]==14.2.0")
+ .add_local_dir(ROOT / "src/flybrain", "/project/src/flybrain", ignore=["**/__pycache__/**"])
+ .add_local_file(ROOT / "tests/test_cuda_hardware.py", "/project/tests/test_cuda_hardware.py")
+ .add_local_file(ROOT / "scripts/validate_cuda.py", "/project/scripts/validate_cuda.py")
+ .add_local_file(
+ ROOT / "models/male-cns-escape-v1/model.json",
+ "/project/models/male-cns-escape-v1/model.json",
+ )
+ .env({"PYTHONPATH": "/project/src"})
+)
+app = modal.App("flybrain-cuda-validation", image=image)
+
+
+@app.function(
+ gpu="T4",
+ cpu=1,
+ memory=4096,
+ timeout=600,
+ retries=0,
+ max_containers=1,
+ min_containers=0,
+ scaledown_window=2,
+)
+def validate():
+ import subprocess
+ import sys
+
+ path = Path("/tmp/cuda-validation.json")
+ result = subprocess.run(
+ [sys.executable, "/project/scripts/validate_cuda.py", "--output", str(path)],
+ cwd="/project",
+ timeout=480,
+ )
+ report = json.loads(path.read_text()) if path.exists() else {"status": "failed"}
+ report["runner_exit_code"] = result.returncode
+ return report
+
+
+@app.local_entrypoint()
+def main(output: str = "cuda-validation.json"):
+ report = validate.remote()
+ Path(output).write_text(json.dumps(report, indent=2) + "\n", encoding="utf-8")
+ print(f"CUDA report: {output}; status: {report['status']}")
+ if report["status"] != "passed" or report["runner_exit_code"] != 0:
+ raise RuntimeError("CUDA validation failed; inspect the report and remote logs")
diff --git a/scripts/validate_fullbrain.py b/scripts/validate_fullbrain.py
new file mode 100644
index 0000000..b72cb0b
--- /dev/null
+++ b/scripts/validate_fullbrain.py
@@ -0,0 +1,336 @@
+"""Opt-in full FlyWire v783 hardware benchmark; never substitutes a subgraph.
+
+Uses the SDK's unmodified CPUBackend / CUDABackend and Connectome classes.
+Download the two official Zenodo files before running. No network access here.
+"""
+
+import argparse
+import gc
+import hashlib
+import json
+import platform
+import time
+from pathlib import Path
+
+import numpy as np
+
+from flybrain.backends.cpu import CPUBackend
+from flybrain.backends.cuda import CUDABackend
+from flybrain.config import LIFConfig, finite_number
+from flybrain.model import Connectome, Synapse
+
+SOURCES = {
+ "proofread_root_ids_783.npy": "e0e6c19732fd8c7a4e39a2d170105421",
+ "proofread_connections_783.feather": "f48f972d262323a102aed49af1396b8a",
+}
+
+
+def digest(path, algorithm="sha256"):
+ h = hashlib.new(algorithm)
+ with Path(path).open("rb") as f:
+ for block in iter(lambda: f.read(8 * 1024 * 1024), b""):
+ h.update(block)
+ return h.hexdigest()
+
+
+def map_source_ids(ids, source_ids):
+ """Match large IDs exactly; mixed uint64/int64 search may promote to float64."""
+ ids, source_ids = np.asarray(ids), np.asarray(source_ids)
+ if ids.dtype.kind not in "iu" or source_ids.dtype.kind not in "iu":
+ raise ValueError("Source neuron IDs must have integer storage")
+ if (ids < 0).any() or (source_ids < 0).any():
+ raise ValueError("Source neuron IDs must be nonnegative")
+ ids = ids.astype(np.uint64, copy=False)
+ source_ids = source_ids.astype(np.uint64, copy=False)
+ indices = np.searchsorted(ids, source_ids)
+ if (indices >= len(ids)).any() or not np.array_equal(ids[indices], source_ids):
+ raise ValueError("Source edge references an unknown neuron")
+ return indices
+
+
+def load_fullbrain(data_dir, *, contact_mv=None):
+ """Load every source row; optional mV weights require a synaptic mV engine.
+
+ Default retains the historical numerical benchmark's normalized weights.
+ Neither weighting policy establishes biological validation.
+ """
+ if contact_mv is not None:
+ contact_mv = finite_number(contact_mv, "contact_mv")
+ if contact_mv <= 0:
+ raise ValueError("contact_mv must be positive")
+ import pyarrow.feather as feather
+
+ sources = {}
+ for filename, expected in SOURCES.items():
+ path = data_dir / filename
+ if digest(path, "md5") != expected:
+ raise ValueError(f"Official source checksum mismatch: {filename}")
+ sources[filename] = {"md5": expected, "sha256": digest(path), "bytes": path.stat().st_size}
+ ids = np.sort(np.load(data_dir / "proofread_root_ids_783.npy", allow_pickle=False))
+ if len(ids) != 139255 or len(np.unique(ids)) != len(ids):
+ raise ValueError("Expected all 139255 unique official proofread IDs")
+ nts = ["ach_avg", "gaba_avg", "glut_avg", "oct_avg", "ser_avg", "da_avg"]
+ columns = ["pre_pt_root_id", "post_pt_root_id", "syn_count", *nts]
+ table = feather.read_table(data_dir / "proofread_connections_783.feather", columns=columns)
+ raw_pre = table[columns[0]].to_numpy()
+ raw_post = table[columns[1]].to_numpy()
+ pre, post = map_source_ids(ids, raw_pre), map_source_ids(ids, raw_post)
+ counts = table["syn_count"].to_numpy().astype(np.int64)
+ if (counts <= 0).any():
+ raise ValueError("Source synapse counts must be positive")
+ # Explicit assumed Dale-like sign per source neuron: weighted mean NT scores
+ # over all outgoing contacts. GABA/glutamate negative; other labels positive.
+ # This approximation does not implement receptors or neuromodulation.
+ scores = np.zeros((len(ids), len(nts)))
+ nonfinite = 0
+ for i, name in enumerate(nts):
+ values = table[name].to_numpy()
+ nonfinite += int((~np.isfinite(values)).sum())
+ scores[:, i] = np.bincount(pre, weights=np.nan_to_num(values) * counts, minlength=len(ids))
+ labels = scores.argmax(axis=1)
+ signs = np.where(np.isin(labels, [1, 2]), -1.0, 1.0)
+ incoming = np.bincount(post, weights=counts, minlength=len(ids))
+ weights = (
+ signs[pre] * 2.5 * counts / incoming[post]
+ if contact_mv is None
+ else signs[pre] * contact_mv * counts
+ )
+ report = {
+ "dataset": "FlyWire v783 / Zenodo 10676866",
+ "source_url": "https://zenodo.org/records/10676866",
+ "sources": sources,
+ "neurons": len(ids),
+ "source_rows": len(table),
+ "retained_rows": len(table),
+ "discarded_rows": 0,
+ "synaptic_contacts": int(counts.sum()),
+ "rows_are": "neuron-pair/neuropil aggregates, retained as parallel weighted edges",
+ "isolated_neurons": int(
+ (
+ np.bincount(pre, minlength=len(ids)) + np.bincount(post, minlength=len(ids)) == 0
+ ).sum()
+ ),
+ "nt_score_nonfinite_entries": nonfinite,
+ "neurons_without_nt_evidence": int((scores.sum(axis=1) == 0).sum()),
+ "assumed_sign_counts": {
+ "positive": int((signs > 0).sum()),
+ "negative": int((signs < 0).sum()),
+ },
+ "weight_policy": (
+ "incoming abs-sum 2.5; GABA/GLUT negative, others/unknown positive"
+ if contact_mv is None
+ else (
+ f"{contact_mv} mV/contact; GABA/GLUT negative, "
+ "others/unknown positive; no normalization"
+ )
+ ),
+ "weight_units": "normalized" if contact_mv is None else "mV",
+ "minimum_contacts": 1,
+ "additional_pruning": False,
+ "graph_arrays_sha256": hashlib.sha256(
+ ids.tobytes()
+ + pre.astype(" 0, "Parity must exercise active spikes, not only rest"
+ report["parity"] = {
+ "ticks": 200,
+ "all_neurons_compared_every": 25,
+ "exact_spikes_and_refractory": True,
+ "atol": 1e-10,
+ "total_spikes": spikes,
+ "checks": parity,
+ }
+ print("Full-network CPU/CUDA parity passed", flush=True)
+ save()
+ checkpoint = json.loads(json.dumps(gpu.snapshot(), allow_nan=False))
+ for tick in range(30):
+ gpu.step(currents[tick % 8])
+ end = gpu.snapshot()
+ gpu.restore(checkpoint)
+ for tick in range(30):
+ gpu.step(currents[tick % 8])
+ assert gpu.snapshot() == end
+ report["checkpoint_replay_exact"] = True
+ timings = {}
+ for name, backend in (("cpu", cpu), ("cuda", gpu)):
+ for _ in range(100):
+ backend.step(currents[0])
+ durations = []
+ for repeat in range(3):
+ start = time.perf_counter()
+ for tick in range(1000):
+ backend.step(currents[(tick // 125) % 8])
+ if name == "cuda":
+ gpu._stream.synchronize()
+ durations.append(time.perf_counter() - start)
+ print(
+ f"{name} repeat {repeat}: {durations[-1]:.3f}s per simulated second", flush=True
+ )
+ timings[name] = {
+ "wall_seconds": durations,
+ "median_seconds": float(np.median(durations)),
+ "simulated_ms_per_repeat": 1000,
+ "warmup_ticks": 100,
+ "includes": "SDK step, host input upload and per-tick error sync",
+ }
+ report["timings"] = timings
+ save()
+ trace, population_spikes = [], 0
+ ever_active = np.zeros(n, dtype=bool)
+ start = time.perf_counter()
+ # Inspect actual device state under its owning stream every tick, accumulating
+ # spikes on device; periodic complete snapshots check finite bounded states.
+ with gpu._device, gpu._stream:
+ active = cp.zeros(n, dtype=cp.bool_)
+ total = cp.zeros((), dtype=cp.int64)
+ for tick in range(args.stability_ticks):
+ gpu.step(currents[(tick // 125) % 8])
+ with gpu._device, gpu._stream:
+ active |= gpu._spikes
+ total += gpu._spikes.sum()
+ if tick % 100 == 99:
+ state = gpu.snapshot()
+ voltage, rates = np.asarray(state["voltage"]), np.asarray(state["rates_hz"])
+ assert np.isfinite(voltage).all() and np.isfinite(rates).all()
+ assert (voltage < 1.0).all() and (rates >= 0).all() and (rates <= 1000).all()
+ trace.append(
+ {
+ "tick": gpu.tick,
+ "voltage_min": float(voltage.min()),
+ "voltage_max": float(voltage.max()),
+ "mean_rate_hz": float(rates.mean()),
+ "spiking_neurons": int(sum(state["spikes"])),
+ }
+ )
+ if tick % 1000 == 999:
+ print(f"Stability {tick + 1}/{args.stability_ticks} ticks", flush=True)
+ with gpu._device, gpu._stream:
+ population_spikes = int(total.get())
+ ever_active = active.get()
+ report["stability"] = {
+ "ticks": args.stability_ticks,
+ "simulated_ms": args.stability_ticks,
+ "wall_seconds_including_observation": time.perf_counter() - start,
+ "total_spikes": population_spikes,
+ "ever_spiking_neurons": int(ever_active.sum()),
+ "trace": trace,
+ }
+ report["gpu_pool_used_bytes"] = cp.get_default_memory_pool().used_bytes()
+ report["gpu_pool_reserved_bytes"] = cp.get_default_memory_pool().total_bytes()
+ report["host_peak_rss_kib_linux"] = None
+ if platform.system() == "Linux":
+ import resource
+
+ report["host_peak_rss_kib_linux"] = resource.getrusage(resource.RUSAGE_SELF).ru_maxrss
+ report["status"] = "passed"
+ except Exception as exc:
+ report["status"] = "failed"
+ report["error"] = f"{type(exc).__name__}: {exc}"
+ raise
+ finally:
+ save()
+ print("FULL BRAIN CUDA VALIDATION PASSED", flush=True)
+
+
+if __name__ == "__main__":
+ main()
diff --git a/scripts/validate_synaptic_cuda.py b/scripts/validate_synaptic_cuda.py
new file mode 100644
index 0000000..2d51733
--- /dev/null
+++ b/scripts/validate_synaptic_cuda.py
@@ -0,0 +1,80 @@
+"""Fail-closed actual-GPU gate for the experimental synaptic mV engine."""
+
+import argparse
+import hashlib
+import json
+import os
+import platform
+import subprocess
+import sys
+import tempfile
+import xml.etree.ElementTree as ET
+from pathlib import Path
+
+
+def main():
+ from flybrain.backends.cuda import _load_cupy
+
+ root = Path(__file__).resolve().parents[1]
+ parser = argparse.ArgumentParser(description=__doc__)
+ parser.add_argument("--output", required=True, type=Path)
+ args = parser.parse_args()
+ report = {
+ "status": "failed",
+ "python": platform.python_version(),
+ "source_sha256": {
+ str(p.relative_to(root)): hashlib.sha256(p.read_bytes()).hexdigest()
+ for p in [
+ *sorted((root / "src/flybrain/experimental").glob("*.py")),
+ root / "tests/test_synaptic_cuda_hardware.py",
+ Path(__file__),
+ ]
+ },
+ }
+ code = 0
+ try:
+ cp = _load_cupy()
+ props = cp.cuda.runtime.getDeviceProperties(cp.cuda.Device().id)
+ report["device"] = {
+ "name": props["name"].decode(),
+ "cupy": cp.__version__,
+ "memory_bytes": props["totalGlobalMem"],
+ "driver": cp.cuda.runtime.driverGetVersion(),
+ "runtime": cp.cuda.runtime.runtimeGetVersion(),
+ }
+ with tempfile.TemporaryDirectory() as directory:
+ xml = Path(directory) / "pytest.xml"
+ subprocess.run(
+ [
+ sys.executable,
+ "-m",
+ "pytest",
+ "tests/test_synaptic_cuda_hardware.py",
+ "-q",
+ f"--junitxml={xml}",
+ ],
+ cwd=root,
+ env={**os.environ, "FLYBRAIN_REQUIRE_CUDA": "1"},
+ check=True,
+ timeout=300,
+ )
+ suites = ET.parse(xml).getroot().iter("testsuite")
+ counts = {k: 0 for k in ("tests", "skipped", "errors", "failures")}
+ for suite in suites:
+ for k in counts:
+ counts[k] += int(suite.get(k, 0))
+ report["cases"] = counts
+ if counts["tests"] != 5 or any(counts[k] for k in ("skipped", "errors", "failures")):
+ raise RuntimeError("all five actual GPU cases must execute and pass")
+ report["status"] = "passed"
+ except Exception as error:
+ report["error"] = f"{type(error).__name__}: {error}"
+ code = 1
+ finally:
+ args.output.parent.mkdir(parents=True, exist_ok=True)
+ args.output.write_text(json.dumps(report, indent=2) + "\n")
+ raise SystemExit(code)
+
+
+if __name__ == "__main__":
+ main()
diff --git a/scripts/validate_synaptic_reference.py b/scripts/validate_synaptic_reference.py
new file mode 100644
index 0000000..95d1877
--- /dev/null
+++ b/scripts/validate_synaptic_reference.py
@@ -0,0 +1,168 @@
+"""Independent Brian2 oracle for the experimental mV synaptic CPU engine.
+
+Install Brian2==2.9.0 and numpy==1.26.4 separately; no Brian2 runtime dependency.
+Tests explicit scheduled input events (not matching two different RNGs).
+"""
+
+import argparse
+import hashlib
+import json
+from pathlib import Path
+
+import numpy as np
+
+from flybrain import Connectome, Synapse
+from flybrain.experimental.synaptic import SynapticCPU, SynapticLIFConfig
+
+
+def compare_case(config):
+ import brian2 as b
+
+ b.start_scope()
+ b.prefs.codegen.target = "numpy"
+ b.defaultclock.dt = config.dt_ms * b.ms
+ # Parallel excitatory edges, inhibitory input, recurrent excitation, autapse,
+ # and a disconnected neuron. Counts below are fixture choices, not fly data.
+ edges = [
+ (0, 2, 40.0),
+ (0, 2, 15.0),
+ (1, 2, -33.0),
+ (2, 3, 55.0),
+ (3, 2, 10.0),
+ (2, 2, 2.0),
+ (3, 4, -20.0),
+ ]
+ model = Connectome(
+ "oracle-fixture",
+ tuple(str(i) for i in range(6)),
+ tuple(Synapse(*edge) for edge in edges),
+ {},
+ {},
+ {},
+ )
+ cpu = SynapticCPU(model, config, input_ids=("0", "1"), weight_units="mV")
+ n_steps = round(100 / config.dt_ms)
+ # Rounding here constructs a known clock-aligned experimental schedule.
+ events = sorted(
+ [(round(t / config.dt_ms), 0) for t in [1.0, 5.0, 10.0, 17.0, 25.0, 40.0]]
+ + [(round(t / config.dt_ms), 1) for t in [6.0, 17.0, 23.0, 40.0]]
+ + [(round(1 / config.dt_ms) + offset, 0) for offset in (1, 2)]
+ )
+ injected = np.zeros((n_steps, 6))
+ for tick, neuron in events:
+ injected[tick, neuron] = 68.75 # 0.275 mV * 250 in the published stimulus.
+ namespace = {
+ "v_rest": config.rest_mv * b.mV,
+ "v_reset": config.reset_mv * b.mV,
+ "v_threshold": config.threshold_mv * b.mV,
+ "tau_mem": config.membrane_tau_ms * b.ms,
+ "tau_syn": config.synapse_tau_ms * b.ms,
+ }
+ # Use the SAME symbol when the time constants are equal so Brian2 can
+ # simplify the degenerate linear system analytically, rather than evaluate
+ # a generic expression containing division by (tau_mem - tau_syn) at zero.
+ syn_tau_symbol = "tau_mem" if config.synapse_tau_ms == config.membrane_tau_ms else "tau_syn"
+ group = b.NeuronGroup(
+ 6,
+ f"""dv/dt = (v_rest - v + g)/tau_mem : volt (unless refractory)
+ dg/dt = -g/{syn_tau_symbol} : volt (unless refractory)
+ rfc : second""",
+ threshold="v > v_threshold",
+ reset="v = v_reset; g = 0*mV",
+ refractory="rfc",
+ method="linear",
+ namespace=namespace,
+ )
+ group.v = config.rest_mv * b.mV
+ group.g = 0 * b.mV
+ group.rfc = config.refractory_ms * b.ms
+ group.rfc[:2] = 0 * b.ms
+ synapses = b.Synapses(
+ group, group, "weight : volt", on_pre="g_post += weight", delay=config.delay_ms * b.ms
+ )
+ synapses.connect(i=[e[0] for e in edges], j=[e[1] for e in edges])
+ synapses.weight = np.array([e[2] for e in edges]) * b.mV
+ source = b.SpikeGeneratorGroup(
+ 2, [e[1] for e in events], np.array([e[0] for e in events]) * config.dt_ms * b.ms
+ )
+ external = b.Synapses(source, group, on_pre="v_post += 68.75*mV")
+ external.connect(i=[0, 1], j=[0, 1])
+ monitor = b.StateMonitor(group, ("v", "g"), record=True, when="end")
+ spikes = b.SpikeMonitor(group)
+ b.Network(group, synapses, source, external, monitor, spikes).run(n_steps * config.dt_ms * b.ms)
+ expected_v = np.array(monitor.v / b.mV).T
+ expected_g = np.array(monitor.g / b.mV).T
+ expected_spikes = np.zeros((n_steps, 6), dtype=bool)
+ expected_ticks = np.rint(np.array(spikes.t / b.ms) / config.dt_ms).astype(int)
+ expected_spikes[expected_ticks, np.array(spikes.i)] = True
+ actual_v, actual_g, actual_spikes = [], [], []
+ checkpoint = None
+ for tick in range(n_steps):
+ cpu.step(injected[tick])
+ observed = cpu.observe_selected(tuple(range(6)))
+ actual_v.append(observed["voltage_mv"])
+ actual_g.append(observed["synaptic_mv"])
+ actual_spikes.append(observed["spikes"])
+ # Save with the first spike still queued for transmission in default case.
+ if tick == round(1 / config.dt_ms) + 2:
+ checkpoint = json.loads(json.dumps(cpu.snapshot(), allow_nan=False))
+ actual_v, actual_g = np.asarray(actual_v), np.asarray(actual_g)
+ np.testing.assert_allclose(actual_v, expected_v, rtol=0, atol=1e-9)
+ np.testing.assert_allclose(actual_g, expected_g, rtol=0, atol=1e-9)
+ np.testing.assert_array_equal(actual_spikes, expected_spikes)
+ assert expected_spikes[:, 2:4].any(), "oracle must exercise downstream propagation"
+ final = cpu.snapshot()
+ cpu.restore(checkpoint)
+ for tick in range(cpu.tick, n_steps):
+ cpu.step(injected[tick])
+ assert cpu.snapshot() == final, "delay-buffer checkpoint replay differs"
+ return {
+ "config": config.to_dict(),
+ "ticks": n_steps,
+ "voltage_max_error_mv": float(np.max(np.abs(actual_v - expected_v))),
+ "synaptic_max_error_mv": float(np.max(np.abs(actual_g - expected_g))),
+ "spikes_per_cell": expected_spikes.sum(axis=0).tolist(),
+ "spike_times_equal": True,
+ "checkpoint_replay_exact": True,
+ }
+
+
+def main():
+ import brian2
+
+ parser = argparse.ArgumentParser(description=__doc__)
+ parser.add_argument("--output", type=Path, required=True)
+ args = parser.parse_args()
+ report = {
+ "status": "running",
+ "oracle": "Brian2",
+ "version": brian2.__version__,
+ "numpy": np.__version__,
+ "cases": [],
+ }
+ args.output.parent.mkdir(parents=True, exist_ok=True)
+ report["implementation_sha256"] = hashlib.sha256(
+ (Path(__file__).parents[1] / "src/flybrain/experimental/synaptic.py").read_bytes()
+ ).hexdigest()
+ report["validation_script_sha256"] = hashlib.sha256(Path(__file__).read_bytes()).hexdigest()
+ try:
+ for config in (
+ SynapticLIFConfig(),
+ SynapticLIFConfig(dt_ms=0.2),
+ SynapticLIFConfig(delay_ms=0),
+ SynapticLIFConfig(synapse_tau_ms=20),
+ ):
+ result = compare_case(config)
+ report["cases"].append(result)
+ print(json.dumps(result), flush=True)
+ report["status"] = "passed"
+ except Exception as error:
+ report["status"] = "failed"
+ report["error"] = f"{type(error).__name__}: {error}"
+ raise
+ finally:
+ args.output.write_text(json.dumps(report, indent=2, allow_nan=False) + "\n")
+
+
+if __name__ == "__main__":
+ main()
diff --git a/site/demos.html b/site/demos.html
index ebf1af5..0c0c076 100644
--- a/site/demos.html
+++ b/site/demos.html
@@ -9,7 +9,7 @@
Observe. Connect. Make it your own.
One Python session loop connects neural activity to a small game environment or synthesized sound. Run a preset, inspect the inputs and controls, then replace an adapter.
Choose the experience: the
game sandbox and
circuit lab run live simulations. The demos below are verified recordings from Python; playback controls inspect a run without recalculating its brain.
-Full FlyWire brain · CUDA benchmark All 139,255 proofread neurons and 16.85 million source rows on a real NVIDIA GPU. CPU/CUDA numerical checks, throughput, checkpoint replay and continuous simulation; this is a full-graph software benchmark, not a trained game policy.
Read the measured results →
Reflex School · reward-trained readout A fixed real 313-neuron circuit, three learned external action weights, and an actual A16 GPU run. On 80 balanced held-out two-choice trials, accuracy changed from 50% to 100%. This is readout learning, not biological plasticity.
Compare before and after →
Godot connectome game adapter
+Full-brain voxel world · recorded GPU experiment All 139,255 FlyWire neurons run on an A16 GPU and control a ground body through an explicit DNa02 readout. Compare the actual trajectory with a silenced-input control. It moves, but curves away from food; no successful navigation or training is claimed.
Watch the voxel experiment →
Full FlyWire brain · CUDA benchmark All 139,255 proofread neurons and 16.85 million source rows on a real NVIDIA GPU. CPU/CUDA numerical checks, throughput, checkpoint replay and continuous simulation; this is a full-graph software benchmark, not a trained game policy.
Read the measured results →
Reflex School · reward-trained readout A fixed real 313-neuron circuit, three learned external action weights, and an actual A16 GPU run. On 80 balanced held-out two-choice trials, accuracy changed from 50% to 100%. This is readout learning, not biological plasticity.
Compare before and after →
Godot connectome game adapter
No CUDA required. Run an actual Godot 4 scene with the Python CPU SDK. Godot owns obstacles and movement; a replaceable encoder and neural readout supply steering. Includes pause, paired world/brain saves, restore and stop-on-disconnect behavior.
Python 3.9+ and Godot 4.7.2 · two terminals
git clone --branch v0.4.0a4 https://github.com/freeman-1984-coder/flybrain-sdk.git
cd flybrain-sdk
diff --git a/site/experiments/fullbrain-synaptic-odor.json b/site/experiments/fullbrain-synaptic-odor.json
new file mode 100644
index 0000000..e13360f
--- /dev/null
+++ b/site/experiments/fullbrain-synaptic-odor.json
@@ -0,0 +1,18441 @@
+{
+ "schema": "flybrain-full-synaptic-odor-v1",
+ "status": "completed",
+ "started_utc": "2026-09-13T02:51:35Z",
+ "config": {
+ "dt_ms": 0.1,
+ "rest_mv": -52.0,
+ "reset_mv": -52.0,
+ "threshold_mv": -45.0,
+ "membrane_tau_ms": 20.0,
+ "synapse_tau_ms": 5.0,
+ "refractory_ms": 2.2,
+ "delay_ms": 1.8,
+ "rate_tau_ms": 50.0
+ },
+ "numpy": "2.5.3",
+ "source_sha256": {
+ "scripts/probe_synaptic_odor.py": "393ae10e825cff56b58cd80f2ab8e559b55cec600886ae85131427545d1cffdf",
+ "scripts/validate_fullbrain.py": "4d543ed43e38cbed609d0cd54003f76049347844d7fa8af58d1ac25753157370",
+ "scripts/build_olfactory_map.py": "00c87876ce4dd1475f225333ea5776b33f1473da6d98157f21f3fd3f98efa8ae",
+ "src/flybrain/experimental/__init__.py": "570d34f551fd63b33bff564c022ad01fc463b0e1649d9f7035e361b421533bd5",
+ "src/flybrain/experimental/synaptic.py": "4c3b7b48e659314a1dd839819ddce4bdd1622d21dd8753400035c1df8deaa726",
+ "src/flybrain/experimental/synaptic_cuda.py": "8b0bc40f8bf0075e6d81caadf3393673110aa48b66415303a08020cb9055dfa8"
+ },
+ "protocol": {
+ "pre_ms": 100,
+ "odor_ms": 500,
+ "post_ms": 200,
+ "trace_interval_ms": 10,
+ "seed": 20260913,
+ "rng": "NumPy PCG64",
+ "input_rate_hz": 150.0,
+ "input_jump_mv": 68.75,
+ "contact_mv": 0.275,
+ "background_input": 0,
+ "sensory_model": "Bernoulli p=rate*dt, at most one input event/cell/tick",
+ "stimulus": "Or42b/DM1 single-channel food-odor approximation",
+ "calibration": "nominal research parameters; not a measured banana dose",
+ "input_refractory_ms": 0,
+ "parameter_source": "https://github.com/philshiu/Drosophila_brain_model/blob/91bdd1e7dcf193f3e7ca5a8933497fcef63b7960/model.py"
+ },
+ "conditions": [
+ {
+ "name": "no_odor",
+ "silenced": false,
+ "input_events": [
+ 0,
+ 0
+ ],
+ "trace": [
+ {
+ "time_ms": 10.0,
+ "groups": {
+ "ORN_DM1_left": {
+ "mean_rate_hz": 0.0,
+ "max_voltage_mv": -52.0,
+ "cumulative_spikes": 0
+ },
+ "ORN_DM1_right": {
+ "mean_rate_hz": 0.0,
+ "max_voltage_mv": -52.0,
+ "cumulative_spikes": 0
+ },
+ "DNa02_left": {
+ "mean_rate_hz": 0.0,
+ "max_voltage_mv": -52.0,
+ "cumulative_spikes": 0
+ },
+ "DNa02_right": {
+ "mean_rate_hz": 0.0,
+ "max_voltage_mv": -52.0,
+ "cumulative_spikes": 0
+ },
+ "ALPN": {
+ "mean_rate_hz": 0.0,
+ "max_voltage_mv": -52.0,
+ "cumulative_spikes": 0
+ },
+ "MBON": {
+ "mean_rate_hz": 0.0,
+ "max_voltage_mv": -52.0,
+ "cumulative_spikes": 0
+ },
+ "descending": {
+ "mean_rate_hz": 0.0,
+ "max_voltage_mv": -52.0,
+ "cumulative_spikes": 0
+ }
+ }
+ },
+ {
+ "time_ms": 20.0,
+ "groups": {
+ "ORN_DM1_left": {
+ "mean_rate_hz": 0.0,
+ "max_voltage_mv": -52.0,
+ "cumulative_spikes": 0
+ },
+ "ORN_DM1_right": {
+ "mean_rate_hz": 0.0,
+ "max_voltage_mv": -52.0,
+ "cumulative_spikes": 0
+ },
+ "DNa02_left": {
+ "mean_rate_hz": 0.0,
+ "max_voltage_mv": -52.0,
+ "cumulative_spikes": 0
+ },
+ "DNa02_right": {
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+ "bilateral_descending_spikes": 2883,
+ "interpretation": "Report includes absent responses. Descending spikes alone do not prove odor-directed navigation. No banana recognition, flight or learning tested."
+ },
+ "finished_utc": "2026-09-13T03:01:55Z"
+}
diff --git a/site/experiments/fullbrain-voxel.json b/site/experiments/fullbrain-voxel.json
new file mode 100644
index 0000000..fdf1ebd
--- /dev/null
+++ b/site/experiments/fullbrain-voxel.json
@@ -0,0 +1,12574 @@
+{
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+ },
+ "counts": {
+ "ORN_DM1_left": 35,
+ "ORN_DM1_right": 33,
+ "DNa02_left": 1,
+ "DNa02_right": 1,
+ "ALPN": 685,
+ "MBON": 96,
+ "descending": 1303
+ }
+ },
+ "mapping_input_units_note": "Actual input units and strengths are in protocol.",
+ "device": {
+ "name": "NVIDIA A16-8Q",
+ "memory_bytes": 8394047488,
+ "cupy": "14.2.0",
+ "driver": 12040,
+ "runtime": 12090
+ },
+ "finished_utc": "2026-09-13T03:14:25Z"
+}
diff --git a/site/foraging.html b/site/foraging.html
new file mode 100644
index 0000000..dff590c
--- /dev/null
+++ b/site/foraging.html
@@ -0,0 +1,27 @@
+
+
+
+
+Full-brain voxel odor experiment · flybrain-sdk
+
+
+
+
+
+
+
+把完整脑,放进一个小世界。
+地上的香蕉代表一个简化食物气味源。两侧触角把浓度变成神经输入;完整 FlyWire 网络在 NVIDIA GPU 上演算,DNa02 神经活动经过明确的动作映射,控制方块世界里的步行身体。
+GPU 实验录制 · 浏览器回放 · No CUDA required to view
+All 139,255 FlyWire neurons and 16,847,997 connection rows run on an NVIDIA A16 GPU. The fixed neural readout moves the body but fails to reach food; silencing sensory inputs leaves it stationary. This is recorded simulation, not live inference or learned navigation.
+完整 FlyWire 网络 —
本组是否接触食物 —
最终距食物 / 游戏单位 —
+实验组 等待实验记录 回放速度 0.1× 慢放 0.25× 1× 模拟时间
+使用下方实验摘要和 JSON 记录查看结果。 正在读取实际 GPU 记录……
+播放 从头回放 时间 0 / 2000 ms
+绿色:实际轨迹 黄色:食物气味源 拖动场景:旋转观察角度
+如何读这个实验 比较正常神经输入和关闭嗅觉入口的同初始状态对照。轨迹来自实际记录;网页不会让角色自动朝向香蕉。
结果以记录为准,包括没有接近或接触食物的情况。
+哪些是连接组,哪些是我们的假设? 全部脑神经元和连接来自 FlyWire v783。输入只覆盖一个 DM1 食物气味通道,不代表完整香蕉气味或视觉识别。脑模型采用简化的突触 LIF 动力学。
动作层是未经训练的工程映射:DNa02 两侧放电频率之差控制转向、频率之和控制速度。尤其是速度映射没有生物学验证。身体是地面运动模型,没有飞行、肌肉或六足步态仿真。记录中的移动不等于可靠觅食。
下载全部帧与参数 · 复现方法与限制 · 开源仓库
+页面只播放实验数据,不在浏览器里运行全脑 CUDA。
+
diff --git a/site/fullbrain.html b/site/fullbrain.html
index 9fcb1bd..d78885c 100644
--- a/site/fullbrain.html
+++ b/site/fullbrain.html
@@ -9,19 +9,35 @@
这次加载全部已校对的 FlyWire v783 脑神经元,运行 SDK 自己的 CUDA 后端。没有抽取小回路,也没有额外删除弱连接。原始报告、校验值和复现脚本均公开。
全部已校对神经元 139,255 包含无连接的神经元
保留的连接表行 16,847,997 按神经元对与脑区汇总
这些行代表的突触接触 54,492,922 官方表内的接触计数总和
通过了什么: 完整图的 LIF 仿真、全体神经元的 CPU/CUDA 数值对照、状态恢复,以及连续运行检查。范围: 使用真实解剖连接,动力学、输入和递质作用是简化假设。这不是完整果蝇行为或意识的复现,也不是训练成果。
-新增功能验证: 气味入口已激活,但基准测试权重阻止了下游放电。 当前配置不能用于证明果蝇会追逐食物。
+新的全脑实验: 采用经验证的突触动力学后,气味刺激已传到下降神经元。 动作方向与可靠觅食仍需独立验证。
模拟 1 秒,需要多久? 同一台 NVIDIA A16-8Q 服务器、同一个网络和输入。预热后各测三次,以下取中位数;加载与编译单独计时。
本次 CUDA 吞吐为 CPU 的 9.61 倍 。计时包含逐步输入上传和错误同步,不包括数据下载、模型加载、编译和网页渲染。这个数字仅适用于此配置和测试输入。
逐项核对 两份原始文件通过官方 MD5 校验;报告另存 SHA-256。 16,847,997 行原始连接全部保留,额外丢弃 0 行;最低接触数为 1。 先验证 20 步静息,再比较 200 步有活动输入;每 25 步检查全部神经元。脉冲和不应期计数完全一致,电压最大差异 0。 完整后端状态通过 JSON 往返,恢复后 30 步结果完全复现。 另外连续运行 10,000 步,即 10 秒模拟时间;每 100 步检查全部状态,未出现非有限或越界状态。 连续运行期间记录 93,609,984 次脉冲,139,254 个神经元至少放电一次。输入是人工电流,不是视觉重建。
实际资源占用 测试卡显存 7.82 GiB;结束时 CuPy 内存池在用约 200.8 MiB。这不包含驱动和全部 CUDA 上下文开销,也不代表显存峰值。进程主机内存峰值约 6.79 GiB;当前导入器使用 Python 对象,因此主机内存开销明显大于纯图数组。
-气味输入:能激活入口,还不能驱动觅食
+打开完整脑方块环境回放 → 正常神经输入驱动了移动,但当前动作映射让角色偏离食物;关闭输入的对照保持静止。
+新模型:气味信号已经传到下游
+2026 年 9 月 13 日,完整 FlyWire 网络在真实 A16-8Q 上换用独立验证的突触 LIF 模型:包含突触衰减、1.8 毫秒传递延迟和明确的毫伏单位。保留所有连接,不再使用阻止信号传播的输入权重归一化。
+
+信号传播已观察到,但不能直接推断会追食物。 左右气味都产生了 DNa02 左侧较强的响应,没有出现简单的左右反转。这是当前动作映射需要解决的问题,不能在网页里悄悄补上自动寻路。
+这里比较的是累计脉冲。刺激刚开始时存在短暂的左右差异,因此不能断言完全没有方向信息。另外,双侧刺激停止后的 200 毫秒内,下降神经元仍新增了 859 次脉冲;这种持续活动需要进一步校准,不能直接称作生物记忆。
+新引擎通过 5 组真实 GPU 检查;完整图在 200 个有嗅觉输入的时间步中,5 个检查点的全体神经元电压、突触状态和频率最大差异为 0。全脑状态连同输入随机数状态,恢复后 20 步完全重现。同机 204 项软件测试通过,零跳过。
+每组包含 100 毫秒静息、500 毫秒刺激、200 毫秒恢复,以 0.1 毫秒步长运行。每组实际耗时约 102–104 秒,包含记录开销。这不是实时模拟,也不与上方不同参数的旧基准直接比较。输入是 Or42b/DM1 单通道食物气味近似,尚未校准为香蕉的真实化学剂量。
+下载新模型五组原始记录 来源、参数与复现证据 ↗
+Full FlyWire synaptic CUDA experiment: sensory-only input reaches ALPN, MBON and descending populations; matched no-odor and silenced-input controls remain silent. DNa02 laterality does not reverse with stimulus side. Navigation is not established.
+旧基准配置:为什么信号停在入口
2026 年 9 月 13 日,在 NVIDIA A16-8Q 上运行相同的完整 139,255 神经元网络,只刺激经标注确认的 68 个 ORN_DM1 感觉神经元。每组从同一静息状态开始,包含 100 毫秒静息、500 毫秒刺激和 200 毫秒恢复。
输入近似乙酸乙酯响应中的 Or42b 单个通道,不是完整的香蕉气味,也不是视觉识别。
结果:入口刺激和关闭操作有效,但这组参数没有产生下游放电。 嗅觉投射神经元在采样时刻观察到的最高电压约 0.252,阈值为 1;有微弱膜电位响应,不等于完全没有信号。
原因已经定位到基准测试的权重配置:每个神经元的输入连接绝对权重之和被归一化到 2.5。在当前不应期下,即使上游以允许的最高频率同步放电,无外部电流的下游电压上限也只有 0.918,低于阈值 1 。因此不能靠提高气味强度解决,也不能把之前的数值测试当作已验证的感觉—动作模型。
-这次 GPU 环境中 181 项软件测试通过。数值验证通过与行为验证通过是不同的结论。下一步是引入并验证有依据的突触衰减、传递延迟与感觉刺激,再连接三维环境中的运动输出。
+这次 GPU 环境中 181 项软件测试通过。数值验证通过与行为验证通过是不同的结论。上方的新模型实验已经补充并验证突触衰减、传递延迟与感觉刺激;本节保留旧配置的负面结果,便于复现和比较。
下载五组完整实验记录 参数诊断与复现说明 ↗
Full-brain olfactory CUDA probe: sensory inputs fire, downstream spiking absent with the benchmark normalization. This is a recorded negative functional result, not live inference, banana recognition, or successful navigation.
开发者如何复现 使用实验分支 feat/cuda-reference,按复现指南 下载官方数据并安装可选 CUDA 依赖。
diff --git a/site/index.html b/site/index.html
index b2ead23..3a04e9e 100644
--- a/site/index.html
+++ b/site/index.html
@@ -17,4 +17,4 @@
brain.save("brain.checkpoint.json")
restored = FlyBrain.restore(
"brain.checkpoint.json"
-)What runs today: a synthetic offline circuit and an opt-in, 313-neuron real MaleCNS subgraph on your CPU. Select cells, inject currents, inspect activity and bind your own output channels. Try it in the browser, export a recording for Python replay, or download an editable HTML demo. The real model uses assumed dynamics. The experimental CUDA branch now has a
complete FlyWire brain benchmark: 139,255 neurons . The released package remains CPU-only; WASM is planned.
A small API, from input to action
Keep the game loop yours. 01 / STIMULATE
Send a sensory signal Food, looming on the left or right, and touch are mapped to explicit inputs in the demo circuit.
02 / STEP
Advance neural time Run fixed simulation ticks on NumPy. Inspect voltages, spikes and firing rates. No GPU setup.
03 / ACT
Read motor intensity Bind your own named output channels, or use the toy walk, turn and jump values. Save an experiment and continue its trajectory.
A model catalog, not a giant install
Download only what you want to use. Source URLs, versions, sizes and licenses live in a lightweight catalog. Assets stream to a local cache when you explicitly request them.
Explore model downloads and limitations →
Build in the open
The next circuit could be yours. Contribute a model source, a real-data importer, an engine adapter or a WASM implementation. You can start with a laptop and a focused pull request.
Start contributing