diff --git a/pkg/analysis/passes/coderules/coderules_test.go b/pkg/analysis/passes/coderules/coderules_test.go
index 6c91aca0..863d51fa 100644
--- a/pkg/analysis/passes/coderules/coderules_test.go
+++ b/pkg/analysis/passes/coderules/coderules_test.go
@@ -728,3 +728,52 @@ func TestNoAPIDsQueryEndpointGood(t *testing.T) {
 	require.NoError(t, err)
 	require.Len(t, interceptor.Diagnostics, 0)
 }
+
+func TestBackendDatasourceShouldNotImplementTestDatasource(t *testing.T) {
+	if !isSemgrepInstalled() {
+		t.Skip("semgrep not installed, skipping test")
+		return
+	}
+	var interceptor testpassinterceptor.TestPassInterceptor
+	pass := &analysis.Pass{
+		RootDir: filepath.Join("./"),
+		ResultOf: map[*analysis.Analyzer]interface{}{
+			sourcecode.Analyzer: filepath.Join("testdata", "backend-datasource-testdatasource-bad"),
+		},
+		Report: interceptor.ReportInterceptor(),
+	}
+
+	_, err := Analyzer.Run(pass)
+	require.NoError(t, err)
+	require.Len(t, interceptor.Diagnostics, 1)
+	require.Equal(t, analysis.Error, interceptor.Diagnostics[0].Severity)
+	require.Equal(
+		t,
+		"Backend datasources (classes extending DataSourceWithBackend) must not implement testDatasource(). Remove this method and rely on backend health checks.",
+		interceptor.Diagnostics[0].Title,
+	)
+	require.Equal(
+		t,
+		"code-rules-backend-datasource-should-not-implement-testdatasource",
+		interceptor.Diagnostics[0].Name,
+	)
+}
+
+func TestBackendDatasourceWithoutTestDatasourceGood(t *testing.T) {
+	if !isSemgrepInstalled() {
+		t.Skip("semgrep not installed, skipping test")
+		return
+	}
+	var interceptor testpassinterceptor.TestPassInterceptor
+	pass := &analysis.Pass{
+		RootDir: filepath.Join("./"),
+		ResultOf: map[*analysis.Analyzer]interface{}{
+			sourcecode.Analyzer: filepath.Join("testdata", "backend-datasource-testdatasource-good"),
+		},
+		Report: interceptor.ReportInterceptor(),
+	}
+
+	_, err := Analyzer.Run(pass)
+	require.NoError(t, err)
+	require.Len(t, interceptor.Diagnostics, 0)
+}
diff --git a/pkg/analysis/passes/coderules/semgrep-rules.yaml b/pkg/analysis/passes/coderules/semgrep-rules.yaml
index d3d1861a..86316805 100644
--- a/pkg/analysis/passes/coderules/semgrep-rules.yaml
+++ b/pkg/analysis/passes/coderules/semgrep-rules.yaml
@@ -353,3 +353,12 @@ rules:
     message: "Direct frontend usage of '/api/ds/query' is not permitted. Use Grafana runtime APIs getDataSourceSrv().get(...) and then query(...) instead of calling this endpoint directly."
     languages: [javascript, typescript]
     severity: ERROR
+
+  - id: backend-datasource-should-not-implement-testdatasource
+    pattern-regex: (?s)class\s+\w+\s+extends\s+DataSourceWithBackend(?:<[^>]+>)?\s*\{.*?\btestDatasource\s*\(
+    paths:
+      include:
+        - "**/datasource.ts"
+    message: "Backend datasources (classes extending DataSourceWithBackend) must not implement testDatasource(). Remove this method and rely on backend health checks."
+    languages: [typescript]
+    severity: ERROR
diff --git a/pkg/analysis/passes/coderules/testdata/backend-datasource-testdatasource-bad/src/datasource.ts b/pkg/analysis/passes/coderules/testdata/backend-datasource-testdatasource-bad/src/datasource.ts
new file mode 100644
index 00000000..4acc3957
--- /dev/null
+++ b/pkg/analysis/passes/coderules/testdata/backend-datasource-testdatasource-bad/src/datasource.ts
@@ -0,0 +1,10 @@
+import { DataSourceWithBackend } from '@grafana/runtime';
+
+export class DataSource extends DataSourceWithBackend {
+  async testDatasource() {
+    return {
+      status: 'success',
+      message: 'ok',
+    };
+  }
+}
diff --git a/pkg/analysis/passes/coderules/testdata/backend-datasource-testdatasource-good/src/datasource.ts b/pkg/analysis/passes/coderules/testdata/backend-datasource-testdatasource-good/src/datasource.ts
new file mode 100644
index 00000000..f546883f
--- /dev/null
+++ b/pkg/analysis/passes/coderules/testdata/backend-datasource-testdatasource-good/src/datasource.ts
@@ -0,0 +1,3 @@
+import { DataSourceWithBackend } from '@grafana/runtime';
+
+export class DataSource extends DataSourceWithBackend {}