Create an RNA-seq workflow script using nextflow with the following properties:
- Download a sample from SRA database from input as a SRA ID using fasterqdump and output as fastq.gz file
- Perform quality control of the raw reads from step 1 using fastp with minimum phred score of 20
- Perform read mapping against human reference genome using STAR with input the filtered reads from step 2 and output aligment file in BAM format
- Perform feature counting at the gene level using featureCounts tool from the alignment BAM file from step 3
- Execute multiqc tool to generate a HTML report of log files from all steps executed above
All tools should be executed in different docker containers
Create an RNA-seq workflow script using nextflow with the following properties:
All tools should be executed in different docker containers