diff --git a/.zenodo.json b/.zenodo.json
new file mode 100644
index 0000000..aab3d70
--- /dev/null
+++ b/.zenodo.json
@@ -0,0 +1,30 @@
+{
+  "title": "pyLocusZoom: Python library for multi-species GWAS visualization",
+  "description": "Publication-ready GWAS visualization in Python: regional association plots with LD coloring, gene tracks and recombination overlays, plus Manhattan, QQ, Miami, eQTL, fine-mapping, PheWAS, forest, LD heatmap and colocalization plots. Built-in canine and feline reference data, Ensembl gene annotation for any species, and matplotlib, plotly and bokeh backends.",
+  "upload_type": "software",
+  "license": "GPL-3.0-or-later",
+  "access_right": "open",
+  "creators": [
+    {
+      "name": "Denyer, Michael"
+    }
+  ],
+  "keywords": [
+    "gwas",
+    "genetics",
+    "visualization",
+    "locuszoom",
+    "regional-plot",
+    "linkage-disequilibrium",
+    "canine",
+    "feline"
+  ],
+  "related_identifiers": [
+    {
+      "identifier": "https://pypi.org/project/pylocuszoom/",
+      "relation": "isSupplementTo",
+      "resource_type": "software",
+      "scheme": "url"
+    }
+  ]
+}
diff --git a/CITATION.cff b/CITATION.cff
new file mode 100644
index 0000000..716e4fc
--- /dev/null
+++ b/CITATION.cff
@@ -0,0 +1,23 @@
+cff-version: 1.2.0
+message: "If you use pyLocusZoom in your research, please cite it using the metadata in this file."
+type: software
+title: "pyLocusZoom: Python library for multi-species GWAS visualization"
+abstract: "Publication-ready GWAS visualization in Python: regional association plots with LD coloring, gene tracks and recombination overlays, plus Manhattan, QQ, Miami, eQTL, fine-mapping, PheWAS, forest, LD heatmap and colocalization plots. Built-in canine and feline reference data, Ensembl gene annotation for any species, and matplotlib, plotly and bokeh backends."
+authors:
+  - family-names: Denyer
+    given-names: Michael
+    email: code.denyer@gmail.com
+version: 3.1.0
+date-released: 2026-09-08
+license: GPL-3.0-or-later
+repository-code: https://github.com/michael-denyer/pyLocusZoom
+url: https://github.com/michael-denyer/pyLocusZoom
+keywords:
+  - gwas
+  - genetics
+  - visualization
+  - locuszoom
+  - regional-plot
+  - linkage-disequilibrium
+  - canine
+  - feline
diff --git a/README.md b/README.md
index 838d384..c9b1ba5 100644
--- a/README.md
+++ b/README.md
@@ -825,6 +825,19 @@ Optional:
 - [Example Notebook](examples/getting_started.ipynb) - Interactive tutorial
 - [CHANGELOG](CHANGELOG.md) - Version history
 
+## Citation
+
+If you use pyLocusZoom in your research, please cite it. GitHub's "Cite this repository" button reads [CITATION.cff](CITATION.cff), and each GitHub release is archived on Zenodo with its own DOI.
+
+```bibtex
+@software{denyer_pylocuszoom,
+  author  = {Denyer, Michael},
+  title   = {pyLocusZoom: Python library for multi-species GWAS visualization},
+  url     = {https://github.com/michael-denyer/pyLocusZoom},
+  license = {GPL-3.0-or-later}
+}
+```
+
 ## License
 
 GPL-3.0-or-later
diff --git a/docs/DEVELOPMENT.md b/docs/DEVELOPMENT.md
index b7b18d1..64d5322 100644
--- a/docs/DEVELOPMENT.md
+++ b/docs/DEVELOPMENT.md
@@ -165,11 +165,13 @@ For a concrete example, see `prepare_manhattan_frames` in
    description covering *what* changed and *why*, and reference any related GitHub
    issues. Do not include AI-assistant attribution in commit messages or PR bodies.
 9. Releases are cut from `main` by bumping `version` in `pyproject.toml`, running `uv lock`,
-   changing the `## [Unreleased]` CHANGELOG heading to `## [X.Y.Z] - YYYY-MM-DD`, committing
-   `pyproject.toml`, `uv.lock`, and `CHANGELOG.md` together, and creating a GitHub release
+   changing the `## [Unreleased]` CHANGELOG heading to `## [X.Y.Z] - YYYY-MM-DD`, setting
+   `version` and `date-released` in `CITATION.cff` to match, committing
+   `pyproject.toml`, `uv.lock`, `CHANGELOG.md`, and `CITATION.cff` together, and creating a GitHub release
    with tag `vX.Y.Z`. `.github/workflows/publish.yml` then publishes to PyPI via Trusted
    Publishing. BiocondaBot opens a follow-up PR against bioconda-recipes automatically once
-   the PyPI release is detected.
+   the PyPI release is detected. Zenodo archives the release and mints a version DOI from
+   `.zenodo.json`; the concept DOI in the README badge covers every version.
 
    Two things that catch people out:
    - `uv.lock` does not regenerate itself on a version bump. Skipping `uv lock` leaves a