barplotModuleEigenGene() Error w/ Tutorial and Data - colorBar

[park-minjun]

I'm running analyseWGCNA() from Quick_Start.ipynb and I run across this error even with the tutorial:

----> 1 pyWGCNA_5xFAD.analyseWGCNA()

File ~/***/.venv/lib/python3.12/site-packages/PyWGCNA/wgcna.py:470, in WGCNA.analyseWGCNA(self, order, geneList, show, alternative)
468 else:
469 for module in modules:
--> 470 self.barplotModuleEigenGene(module, metadata, colorBar=metadata[-1], show=show)
471 print("\tDone..\n")
473 if self.save:

File ~/***/.venv/lib/python3.12/site-packages/PyWGCNA/wgcna.py:3133, in WGCNA.barplotModuleEigenGene(self, moduleName, metadata, combine, colorBar, show)
3131 else:
3132 palette = cat[[colorBar]].copy()
-> 3133 palette[[colorBar]] = palette[[colorBar]].astype('int')
3134 palette[colorBar] = palette[colorBar].apply(lambda x: mcolors.to_hex(self.metadataColors[colorBar].to_rgba(float(x))) if pd.notna(x) else "#FFFFFF")
3135 palette = palette[colorBar].values

File ~/***/.venv/lib/python3.12/site-packages/pandas/core/generic.py:6662, in NDFrame.astype(self, dtype, copy, errors)
6656 results = [
6657 ser.astype(dtype, copy=copy, errors=errors) for _, ser in self.items()
6658 ]
6660 else:
6661 # else, only a single dtype is given
...
132 # Explicit copy, or required since NumPy can't view from / to object.
--> 133 return arr.astype(dtype, copy=True)
135 return arr.astype(dtype, copy=copy)

ValueError: invalid literal for int() with base 10: '5xFADHEMI'

All metadata properly updated, followed step by step. Tracing back I'm not sure why colorBar argument is set as metadata[-1] as metadata seems to be self.datExpr.obs.columns.tolist() which makes this a list of columns and the colorBar is theoretically expecting a dictionary.

Thank you so much for your help!

[mattjmeier]

I'm having this same problem - did you ever figure out a way to get the analysis to proceed?