Hi!
Thank you for your tool, it has been very helpful!
I was using your library to generate module genes. I set the following parameters in the PyWGCNA.WGCNA function:
pyWGCNA_Seleccionados = PyWGCNA.WGCNA(name='Seleccionados', geneExp = df_tpm, sampleInfo = metadata, minModuleSize = 50)
When I computed the modules, I noticed that some of them (that were not the grey) had less than 50 genes even though I set 50 genes as the minimum number of genes for the modules.
Is this normal behavior? Can I trust and use this modules with less than the minimum set for downstream analysis?
Thank you in advance :)
Hi!
Thank you for your tool, it has been very helpful!
I was using your library to generate module genes. I set the following parameters in the PyWGCNA.WGCNA function:
pyWGCNA_Seleccionados = PyWGCNA.WGCNA(name='Seleccionados', geneExp = df_tpm, sampleInfo = metadata, minModuleSize = 50)When I computed the modules, I noticed that some of them (that were not the grey) had less than 50 genes even though I set 50 genes as the minimum number of genes for the modules.
Is this normal behavior? Can I trust and use this modules with less than the minimum set for downstream analysis?
Thank you in advance :)