diff --git a/requirements.txt b/requirements.txt
index 4836630..6cc19b3 100644
--- a/requirements.txt
+++ b/requirements.txt
@@ -17,7 +17,7 @@ numpy==1.18.1
 packaging==20.1           # via pytest
 pandas==0.25.3
 pluggy==0.13.1            # via pytest
-py==1.8.1                 # via pytest
+py==1.10.0                 # via pytest
 path.py==12.0.2           # via pytest-shutil
 pyfaidx==0.5.8
 pyfastaq==3.17.0          # via shell scripts