paper

[aliechoes]

Hi

For which paper this package is developed?

[MaximLippeveld]

Hi, this for the paper Classification of Human White Blood Cells Using Machine Learning for Stain‐Free Imaging Flow Cytometry. Thanks for your interest!

[aliechoes]

thanks for the reply. I actually found your paper quite interesting. I am also working on IFC as well. so it was a quite good to see your work.
cheers

[aliechoes]

Thanks again for the reply. I found the paper quite interesting. I am wondering whether the data is publicly available for experiments?

[MaximLippeveld]

Hi, glad you liked it! The data is available. This directory contains everything used in the paper.

[aliechoes]

awesome! thanks :)

[aliechoes]

Hi Maxim, So I download the files and try to use cifconvert. However it seemed quite challenging to convert the files. Also, I was not able to open the .daf files using IDEAS. Not sure what was the problem.
I am wondering if the hdf5 or .tif files are somewhere stored which I can use directly?
in addition, can you please specificy which channels are used? Thanks a lot!

[MaximLippeveld]

Indeed, cifconvert isn't bug free, unfortunately :-) It should work in most caes, though. What problems did you run into?

What version of IDEAS are you using to open the daf files?

I don't have the hdf5 files available, currently. Can you open the cifs in IDEAS? Then you can export the tif files, maybe.

[aliechoes]

I am using IDEAS 6.2. It tells that there is something wrong with the .daf files though.
I could however open the .cif file alone. but I was not sure if all the cells in the .cif files are the one used in your work. For example, I was not sure whether focused cells were gated or not.

[MaximLippeveld]

I indeed used only single, focused cells. Have you tried re-downloading the .daf files? I will try later today whether I can still open them.