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Use separate modules for flye coassembly and minigraph before rhea #122

@ppreshant

Description

@ppreshant

Goal, tasks

To take full advantage of the nextflow pipeline, we should separate the major tasks into separate modules within the genome_dynamics subworkflow before rhea.

  • run the flye module with all samples under a co-assembly with --keep-haplotypes = true
  • Run minigraph module to align the reads onto the gfa
  • (oops, I got confused minigraph for minimap) Run nf-core/minimap_index (+_align?) module to align the reads onto the gfa
    • (found on nf-core)
  • Change the metromap to reflect this (linearize pre-proc - assembly and fork reads and contigs into the other analysis branches)

References

  • Note on flye params from rhea paper:

    Methods/Graph construction and coverage calculations: A single coassembly graph for the series with N samples is constructed by combining all reads from all samples into one metaFlye run (19), with --keep-haplotypes parameter set to true to maintain strain variations

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    enhancementNew feature or requestnice-to-havefeature requests/enhancements that are not too significant but would be nice to have.

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