May 19–21 2026 • Mezzanine & Lab IN.1P01.040
This course provides a foundational understanding of Fluorescence In Situ Hybridization (FISH), with a focus on single molecule RNA FISH (smFISH).
Participants will explore the core principles behind FISH alongside practical applications including:
- Probe design
- Fluorescent oligo hybridization
- Widefield fluorescence imaging
- Image processing and deconvolution
- Quantitative image analysis
The course combines theory lectures with extensive hands-on laboratory sessions.
Participants will also gain practical experience using:
| Item | Details |
|---|---|
| Location | Mezzanine and Lab IN.1P01.040 |
| Format | Theory lectures + hands-on laboratory sessions |
| Topics Covered | FISH principles, probe design, hybridization, fluorescence imaging, deconvolution, image analysis |
| Time | Activity | Trainer(s) |
|---|---|---|
| 11:00–12:00 | Theory Introduction | Magda Bienko |
| 12:00–13:00 | Probe Design Theory | Giulia Peveri, Erik Wernersson |
| 13:00–14:00 | Lunch Break | — |
| 14:00–15:00 | Hands-on: Probe Design | Giulia Peveri, Erik Wernersson |
| 15:00–15:30 | Probe Production | Nicola Crosetto |
| 15:30–17:30 | Lab 1: Probe Hybridization | Giulia Della Chiara |
| Time | Activity | Trainer(s) |
|---|---|---|
| 09:30–10:45 | Lab 2.1: Washes & Fluorescent Oligo Hybridization | Giulia Della Chiara |
| 10:45–11:00 | Coffee Break ☕ | — |
| 11:00–13:00 | Lab 2.2: Washes & Mounting | Giulia Della Chiara |
| 13:00–14:00 | Lunch Break | — |
| 14:00–15:15 | Introduction to Image Processing & Deconvolution | Erik Wernersson, Giulia Della Chiara |
| 15:15–15:30 | Coffee Break ☕ | — |
| 15:30–17:00 | Hands-on: Deconvolution & FIJI | Erik Wernersson, Giulia Peveri, Giulia Della Chiara |
| Time | Activity | Trainer(s) |
|---|---|---|
| 09:30–10:30 | Theory Imaging: Cell Segmentation & Dot Detection | Erik Wernersson |
| 10:30–11:30 | Lab 3: Imaging — Group 1 | Giulia Della Chiara |
| 11:30–12:30 | Lab 3: Imaging — Group 2 | Giulia Della Chiara |
| 12:30–14:00 | Lunch Break | — |
| 14:00–15:00 | Lab 3: Imaging — Group 3 | Giulia Della Chiara |
| 15:00–16:00 | Hands-on: Image Analysis | Erik Wernersson, Giulia Peveri, Giulia Della Chiara |
| 16:00–17:00 | Closing Remarks & Q&A | Magda Bienko, Nicola Crosetto |
By the end of the course, participants will be able to:
- Understand the theoretical foundations of FISH and smFISH
- Design and produce fluorescent probes
- Perform hybridization and washing procedures
- Acquire fluorescence microscopy images
- Apply deconvolution techniques to microscopy data
- Analyze FISH signals using FIJI and related image processing workflows
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