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Filtering Parameters provide more nuanced filtering of peaks. This is fast, allowing for quick iteration on peak identification without having to rescan the signal for peaks.
Parameter
Description
Min Peak Height
Peaks with peak height lower than Min Peak Height are filtered out
Max Peak Height
Peaks with peak height greater than Max Peak Height are filtered out
Min Peak Height Ratio
Secondary peaks with a height ratio compared to the primary peak less than Min Peak Height Ratio are filtered out
Max Bleedthrough Ratio
Let signal strength be the sum of the signal from -1 to +1 indices of a peak. Bleedthrough ratio is defined as the peak signal strength divided by the maximum signal strength of other channels in the same well at the same peak index. Peaks with a bleedthrough ratio greater than Max Bleedthrough Ratio are filtered out
Max Crosstalk Ratio
Crosstalk ratio is defined as the peak signal strength divided by the maximum signal strength of other channels in the same color in surrounding wells (surrounding wells defined as surrounding capillaries as they feed into the machine, not surrounding wells within the plate.)
Min Peak Distance
Peaks that are less than Min Peak Distance away (in nucleotides) are filtered out, leaving only the tallest peaks