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docs: add PYROGLUTAMATE_RISK + VAR_033 Met to EXPERT_REVIEW_PACK limitations table - #99
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…tations table EXPERT_REVIEW_PACK.md Pipeline Limitations table (§8): - Add SEED-009_VAR_033 Met oxidation row (VAR_033 = RRLPRPGYMPRP, Met at position 9, carries both MET×1 and PROLINE_RICH_INTRACELLULAR; Nle substitution + HPLC purity mandatory) - Add PYROGLUTAMATE_RISK row (N-terminal Q → pGlu at physiological pH, t½ hours-days, 5-50× MIC loss; none of 20 pilot candidates affected; E1 excluded; Nα-acetylation is zero-cost mitigation) - Minor wording fix: 'argon storage' → 'inert atmosphere storage' on the existing SEED-007 Met row (matches actual QC flag text) ROADMAP.md + DISCOVERY_PREDICTION.md: tracking for PR #107 (VAR_033 regression test) and PR #108 (this doc update)
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Summary
Completes the Pipeline Limitations table in
EXPERT_REVIEW_PACK.md §8with two missing rows:New: SEED-009_VAR_033 Met oxidation row
VAR_033 (RRLPRPGYMPRP) is the only SEED-009 pilot with Met at position 9. It carries both
MET×1(Nle recommendation) andPROLINE_RICH_INTRACELLULARsimultaneously. The WET_LAB_HANDOFF was corrected in PR #106; the limitations table is now consistent.New: PYROGLUTAMATE_RISK row
The QC flag added in PR #105 (N-terminal Q → pGlu at pH 7.4, t½ hours–days, 5–50× MIC loss) was not reflected in the limitations table. None of the 20 pilot candidates are affected (no Q-terminal sequences), but the table should document all active QC checks. E1 excluded (acid-catalysed, negligible at physiological pH).
Minor fix: "argon storage" → "inert atmosphere storage" on the existing SEED-007 Met row to match actual QC flag text (PR #102).
Also adds tracking notes for PRs #107 and #108 to ROADMAP.md and DISCOVERY_PREDICTION.md.
Test plan
pytest— 1312/1312 pass (prose-only changes)grep "PYROGLUTAMATE" docs/EXPERT_REVIEW_PACK.mdreturns a row in the limitations table🤖 Generated with Claude Code