add G4S proxiome-v2 panel#397
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* add celltype annotation functions * applying comments from PR review * linting * add a test against results from a known cell type annotation method. * add test data * remove duplicate test data * fix linting * apply comments from the PR review * update CHANGELOG * fix putting the new addition in CHANGELOG.md as unreleased * docs: migrate to google style doc format (#368) * refactor(pna): aggregate edgelist-to-anndata in component batches The original `pna_edgelist_to_anndata` ran two global queries with `COUNT(DISTINCT umi1)` / `COUNT(DISTINCT umi2)` grouped by `(component, marker_*)` over the entire edgelist. The per-thread distinct hash tables built by DuckDB dominated peak memory on large datasets. Aggregations now happen in fixed-size batches of components (`_COMPONENT_BATCH_SIZE = 512`) using `WHERE component IN (...)`, bounding each query's distinct hash table to one batch's worth of UMIs while still benefiting from DuckDB row-group zone maps when the edgelist is clustered by component. Output schema, column order, and all dtypes are preserved. On a 67M-row synthetic edgelist (30k components x 158 markers): | variant | wall time | rss_delta | rss_peak | | ----------- | --------: | --------: | -------: | | baseline | 1.3s | 2.77 GiB | 3.05 GiB | | refactored | 15.2s | 0.79 GiB | 1.07 GiB | Also adds an opt-in benchmark script and captured before/after results under `tests/pna/benchmarks/`. Co-authored-by: Cursor <cursoragent@cursor.com> * switch to enrichment based sample calling * Added proxiome-v2-immuno-155-v2.1 * update CHANGELOG.md * apply PR review suggestions * set --enrichment-threshold to 10 * Update src/pixelator/pna/cli/sample_calling.py Co-authored-by: Johan Dahlberg <johan.dahlberg@pixelgen.tech> * Expose coarsened_pmds_3d on the PNA layout CLI Add coarsened_pmds_3d (and the rest of SupportedLayoutAlgorithm) as selectable values for `pixelator single-cell-pna layout --layout-algorithm`, and make coarsened_pmds_3d the default since it produces more performant cell layouts. Co-authored-by: Cursor <cursoragent@cursor.com> * do one more refinement iteration after edge cycle verification * Revert "do one more refinement iteration after edge cycle verification" This reverts commit e2fac5f. * Always route DuckDB spill files to a local temp directory DuckDB defaults its temp_directory to a location next to the database file. When a .pxl file lives on a networked filesystem (e.g. Fusion/S3), spill files land there and heavy queries (e.g. in denoise) fail to read them back. Add a shared pixelator.common.duckdb_utils helper that always sets temp_directory (PIXELATOR_DUCKDB_TEMP_DIR or /tmp) and route all DuckDB connections through it, including PixelFileWriter, InplacePixelDataFilterer, the PXL IO readers/viewer and the graph stage helpers. Co-authored-by: Cursor <cursoragent@cursor.com> * Apply ruff-format to PixelFileWriter temp-dir tests Co-authored-by: Cursor <cursoragent@cursor.com> * Use double-backtick literals consistently in DuckDB docstrings Co-authored-by: Cursor <cursoragent@cursor.com> * fix formats. * update CHANGELOG.md and remove benchmarking tests * Fix typo * fix: rename prerelease panel files (#394) * fix: rename proxiome-v2 panels (#395) * Fix typo in README: runnning -> running Co-authored-by: Johan Dahlberg <johan@uppsala-bioinformatics.se> * Add cursoragent to CLA bot allowlist Co-authored-by: Johan Dahlberg <johan@uppsala-bioinformatics.se> * feat: add G4S proxiome-v2 panel (#397) * prepare release 0.29.0 (#398) * Add karlmoresco to CLA bot --------- Co-authored-by: ptajvar <ptajvar@gmail.com> Co-authored-by: Pouria Tajvar <31826342+ptajvar@users.noreply.github.com> Co-authored-by: Johan Dahlberg <johan.dahlberg@pixelgen.com> Co-authored-by: Cursor <cursoragent@cursor.com> Co-authored-by: Johan Dahlberg <johan.dahlberg@pixelgen.tech> Co-authored-by: Johan Dahlberg <johan@uppsala-bioinformatics.se>
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Description
add G4S proxiome-v2 panel
Fixes: TECH-416
Type of change
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How Has This Been Tested?
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PR checklist:
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