Add bamCompare support and robust BWA index resolution to ChIP-seq workflow#4
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WilliamLautertD wants to merge 2 commits into
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Add bamCompare support and robust BWA index resolution to ChIP-seq workflow#4WilliamLautertD wants to merge 2 commits into
WilliamLautertD wants to merge 2 commits into
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Motivation
bamCompare) so ChIP samples can be directly compared to their paired Input controls..fabasenames and warn if it auto-corrects a relative path.Description
BAM_COMPAREprocess that runsbamComparewith--ignoreDuplicates,--operation ratio, configurable--binSizeand--smoothLength, and publishes ratio bigWigs tocoverage/bamcompare.chipseq_nextflow.config:make_bamcompare,bamcompare_binsize, andbamcompare_smooth_length, and allocatedwithName: BAM_COMPARECPUs.chipseq_samples.tsvtemplate to include acontrol_samplecolumn and updated the workflow to require acontrol_samplefor non-Input conditions, storing it inmeta.control_sample.resolveBwaReference()andvalidateBwaReference()helper functions that locate BWA index files (.amb/.ann/.bwt/.pac/.sa) for a given basename or try.favariants, setparams.bwa_resolved_reference, and emit a warning if the path was auto-corrected.BWA_MEM_FILTERto useparams.bwa_resolved_reference(or fall back toresolveBwaReference()), and added workflow wiring that joins chip sample BAMs with their Input BAMs and conditionally invokesBAM_COMPAREwhenparams.make_bamcompareis enabled.README.mdto document thecontrol_samplepairing,bwa_index_prefixexpectations, andbamComparedefaults.Testing
Codex Task