Data and Scripts for Statistical Analyses

This repository contains the data and scripts needed to reproduce the statistical analyses conducted for the manuscript:

"Shifts in microbial communities driven by the succession of seagrasses to benthic macroalgae may exacerbate heatwave effects in eutrophicated-coastal lagoons"
— E. Rubio-Portillo et al.

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⚠️ Note on Heavy Computations

Some of the calculations in this project are very time-consuming. To avoid long compilation times, the corresponding chunks in the .Rmd file are set with eval=FALSE. Immediately after, there are chunks that load the precomputed data.

These data files are located in the Auxiliary_Data folder. Before knitting the .Rmd file, make sure to move them to the same directory level as the .Rmd file. Notice that the total size of the data files in the Auxiliary_Data folder is over 5 GB.

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Repository Structure

• Auxiliary_Data/ Precomputed data files, to avoid long compilation times
• Additional-File-2.Rmd Main RMarkdown file
• funcionsCODAMETACIRCLE.R Script with functions used by Additional-File-2.Rmd
• Input_Data/ Data obtained in the experiments
• Main_Results/ Output data tables produced by the statistical analysis
• README.md This file

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Reproducibility Instructions

1. Clone this repository.
2. Move the contents of Input_Data and Auxiliary_Data to the same folder as Additional-File-2.Rmd and funcionsCODAMETACIRCLE.R.
3. Open Additional-File-2.Rmd in RStudio and knit.