Using GC Skew to reorder contigs

[balags1]

It is very useful to know Ori/Ter in draft genomes.
However, is there a way to reorder multiple non-overlapping contigs then, such as from a WGS Illumina sequencing experiment yielding a 30-100 contigs assembly, to match appropriate GC Skew distribution? To do this independently based on the GC skew distribution, without a reference genome?

[christophertbrown]

This is a good question, and agree knowing Ori/Ter can be helpful. We considered this, but found that the signal was too low and the number of possible configurations too great in most cases. If you have a genome in very few piece (e.g. 2-5), it can be helpful. Chris

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On Jun 21, 2021, at 7:31 PM, balags1 ***@***.***> wrote:  It is very useful to know Ori/Ter in draft genomes. However, is there a way to reorder multiple non-overlapping contigs then, such as from a WGS Illumina sequencing experiment yielding a 30-100 contigs assembly, to match appropriate GC Skew distribution? — You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHub, or unsubscribe.