Hi, I'm trying to get PTR value on some super shallow sequencing data and found bPTR doesn't give any value calculated and doesn't plot the log2 filtered coverage figure anymore when the coverage is below certain number (0.34X in my case). Is there a hard cutoff in the software and how can I get rid of it? Just want to get a value for the shallow data to compare with the higher depth data.
Btw, can I run the software on long-reads sam file in the same way?
Thanks,
Yanchun
Hi, I'm trying to get PTR value on some super shallow sequencing data and found bPTR doesn't give any value calculated and doesn't plot the log2 filtered coverage figure anymore when the coverage is below certain number (0.34X in my case). Is there a hard cutoff in the software and how can I get rid of it? Just want to get a value for the shallow data to compare with the higher depth data.
Btw, can I run the software on long-reads sam file in the same way?
Thanks,
Yanchun