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Improvements from our RNA-Seq pipeline#181

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arteymix wants to merge 11 commits intodeweylab:masterfrom
PavlidisLab:gemma-rnaseq-pipeline
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Improvements from our RNA-Seq pipeline#181
arteymix wants to merge 11 commits intodeweylab:masterfrom
PavlidisLab:gemma-rnaseq-pipeline

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@arteymix arteymix commented Feb 22, 2022

Here are a few things we've improved in RSEM for our RNA-Seq pipeline.

  • use move instead of system(mv...) as it works across filesystems
  • use a temporary directory with auto cleanup for storing intermediary files, which is very friendly for HPC systems storing temporary results in scratch
  • export STAR Log.final.out to the final directory
  • fix incorrect examples in rsem-calculate-expression docs

I'm not expecting everything here will be retained, especially the shared memory option for STAR. I can reintroduce the two removed parameters to adjust the temporary dir location and conditionally set CLEANUP.

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