Universal dilution solver. Fast, clear, and lab-ready.
DiluteIt is a lightweight web app for solving the universal dilution equation (C1V1 = C2V2) in a clean, mobile-friendly interface.
It is part of Host Cell, a growing suite of practical laboratory and bioprocess tools built by Emiliano Balderas (IBt-UNAM).
DiluteIt solves one of the most common calculations in biotechnology and lab workflows: dilution planning.
The app lets you solve for any one of the four variables in the dilution equation:
- C1 = stock concentration
- V1 = stock volume (aliquot / inoculum)
- C2 = target final concentration
- V2 = final total volume
You choose the unknown, provide the other three values, and the app returns the result with unit-aware handling.
DiluteIt applies the dilution equation derived from conservation of solute amount:
- C1: concentration of the stock solution
- V1: volume of stock used (aliquot / inoculum)
- C2: desired final concentration
- V2: final total volume
Depending on the unknown variable, the app solves:
DiluteIt supports practical concentration and volume units for routine lab use.
- M, mM, μM (molar basis)
- mg/mL (mass/volume basis)
- % (percentage basis)
- L
- mL
- μL
DiluteIt assumes C1 and C2 use the same concentration basis.
✅ Valid examples:
- M ↔ mM ↔ μM
- mg/mL ↔ mg/mL
- % ↔ %
❌ Not valid without extra information:
- M ↔ mg/mL
- % ↔ M
- mg/mL ↔ % (unless convention + density/purity are defined)
If you need cross-basis conversion (for example, molarity to mg/mL), you must use additional data such as:
- molecular weight (MW)
- density
- purity / assay
- Universal Solver: Choose any of the 4 variables as the unknown (C1, V1, C2, or V2)
- Unit-Aware Inputs: Handles common lab concentration and volume units
- Compatibility Validation: Warns when C1 and C2 use incompatible concentration bases
- Mobile-First UI: High-contrast layout and large controls for real lab use
- Bilingual Interface: Spanish / English toggle
- PWA Ready: Installable on Android/iOS and usable offline after first load
- Host Cell Design System: Visual consistency with other tools in the suite
DiluteIt is useful for:
- preparing working solutions from concentrated stocks
- serial dilution planning (step-by-step use)
- quick aliquot calculations at the bench
- training students on dilution logic
- reducing manual calculator/transcription errors
- Select the unknown variable (C1, V1, C2, or V2)
- Enter the other three known values
- Choose the corresponding units
- Click Solve
- Review the result and verify unit compatibility
If you need to prepare a final solution at C2 from a stock C1:
- Enter C1, C2, V2
- Set V1 as the unknown
- DiluteIt returns the required aliquot volume (V1)
DiluteIt can be installed as a Progressive Web App (PWA) for faster access and offline use.
- Open the live app
- Tap/click Install App (if shown)
- Or use the browser install prompt/menu
- Open the live app
- Tap Share
- Select Add to Home Screen
Once installed, the app can work offline after the required files are cached.
Q: Does DiluteIt convert molarity to mg/mL automatically? A: No. The dilution equation preserves solute amount, but it does not perform cross-basis chemical conversions. You need MW (and sometimes density/purity) for that.
Q: Can I solve for any variable? A: Yes. DiluteIt is a universal solver for C1, V1, C2, or V2, as long as the other three values are valid.
Q: Does it work offline? A: Yes. After the first successful load (and installation, if desired), the service worker caches the app for offline use.
Q: Is it only for bioprocessing? A: No. It was built within a biotech workflow, but the dilution math is broadly useful for many lab contexts.
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DiluteIt is a calculation aid, not a substitute for SOPs or experimental judgment.
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Always verify:
- unit basis compatibility
- concentration conventions (% w/v, v/v, etc.)
- stock identity and labeling
- pipetting feasibility and practical volume limits
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For critical workflows, follow institutional protocols and lab-specific validation practices.
Emiliano Balderas Biotechnology Engineer | PhD Student in Biochemistry Instituto de Biotecnología (IBt) - UNAM
Host Cell is a growing suite of practical lab and bioprocess tools focused on:
- clarity
- speed
- reproducibility
- real-world usability at the bench
DiluteIt is one module in that ecosystem.
Host Cell Lab Suite – Practical tools for high-performance biotechnology.