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4 changes: 2 additions & 2 deletions Help/3 Analysis Modules/1 Basic Statistics.html
Original file line number Diff line number Diff line change
Expand Up @@ -21,8 +21,8 @@ <h2>Summary</h2>
<li>File type: Says whether the file appeared to contain actual base calls or
colorspace data which had to be converted to base calls</li>
<li>Encoding: Says which ASCII encoding of quality values was found in this
file.
</li><li>Total Sequences: A count of the total number of sequences processed.
file.</li>
<li>Total Sequences: A count of the total number of sequences processed.
There are two values reported, actual and estimated. At the moment these
will always be the same. In the future it may be possible to analyse just
a subset of sequences and estimate the total number, to speed up the analysis,
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2 changes: 1 addition & 1 deletion Help/3 Analysis Modules/12 Per Tile Sequence Quality.html
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Expand Up @@ -28,7 +28,7 @@ <h2>Summary</h2>
can see that certain tiles show consistently poor quality. A good
plot should be blue all over.
</p>
<p><img src="per_tile_quality.png" alt="Kmer profiles"></p>
<p><img src="per_tile_quality.png" alt="Per tile quality"></p>
<p>
Reasons for seeing warnings or errors on this plot could be transient
problems such as bubbles going through the flowcell, or they could
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5 changes: 3 additions & 2 deletions Help/3 Analysis Modules/4 Per Base Sequence Content.html
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Expand Up @@ -59,15 +59,15 @@ <h2>Common reasons for warnings</h2>
<ol>
<li>Overrepresented sequences: If there is any evidence of overrepresented
sequences such as adapter dimers or rRNA in a sample then these sequences
may bias the overall composition and their sequence will emerge from this plot.
may bias the overall composition and their sequence will emerge from this plot.</li>
<li>Biased fragmentation: Any library which is generated based on the ligation
of random hexamers or through tagmentation should theoretically have good
diversity through the sequence, but experience has shown that these libraries
always have a selection bias in around the first 12bp of each run. This is
due to a biased selection of random primers, but doesn't represent any individually
biased sequences. Nearly all RNA-Seq libraries will fail this module because of
this bias, but this is not a problem which can be fixed by processing, and it
doesn't seem to adversely affect the ablity to measure expression.
doesn't seem to adversely affect the ablity to measure expression.</li>
<li>Biased composition libraries: Some libraries are inherently biased in their
sequence composition. The most obvious example would be a library which has been
treated with sodium bisulphite which will then have converted most of the cytosines
Expand All @@ -80,6 +80,7 @@ <h2>Common reasons for warnings</h2>
only sequences which do not match. Sudden deviations in composition at the end
of libraries which have undergone aggressive trimming are therefore likely to be
spurious.</li>
</ol>

</body>
</html>
2 changes: 1 addition & 1 deletion Help/3 Analysis Modules/6 Per Base N Content.html
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Expand Up @@ -13,7 +13,7 @@ <h1>Per Base N Content</h1>
<h2>Summary</h2>
<p>
If a sequencer is unable to make a base call with sufficient confidence
then it will normally substitute an N rather than a conventional base]
then it will normally substitute an N rather than a conventional base
call
</p>
<p>
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55 changes: 0 additions & 55 deletions uk/ac/babraham/FastQC/Report/stylesheet.txt

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