To cite this work (datasets, methods, code, results), please refer to the article:
Ugur Dag*, Ijeoma Nwabudike*, Di Kang*, Matthew A. Gomes, Jungsoo Kim, Adam A. Atanas, Eric Bueno, Cassi Estrem, Sarah Pugliese, Ziyu Wang, Emma Towlson, Steven W. Flavell
Cell 2023; doi: https://doi.org/10.1016/j.cell.2023.04.023
*Equal Contribution
The processed data files for our two whole-brain imaging strains (SWF415 and SWF702) are available under the data directory.
Receptor expression, neuron class ID and publicly available connectome data are also provided.
Run nsm_analysis.ipynb to compute each neuron's temporal correlation with NSM for individual animals.
Run generate_figure7.ipynb to relate each neuron's functional relationship with NSM to receptor expression.
Both scripts are available under the notebook directory.
Please follow the instructions to install all prerequisite packages for behavioral tracking, image registration and signal processing: https://github.com/flavell-lab/AtanasKim-Cell2023
The functions that are used specifically for this analysis are available under code.
The outputs of nsm_analysis.ipynb for multiple animals of each imaging strain are compiled as a Julia dictionary under the results directory.
You may reproduce figure 7 of the above paper by directly plugging in a julia dictionary without re-running nsm_analysis.ipynb yourself.