Release 4.1.0

[Aratz]

[4.1.0] - 2026-05-28

Enhancements & fixes

• Update pixelator container to 0.27.1 by @johandahlberg #212
• Denoise graph before sample calling step by @Aratz #212
• Add ACE and PLS denoising by @Aratz #212

Changed

• K1 denoising is turned off by default by @Aratz #212

Software dependencies

Dependency	Old version	New version
pixelator	0.26.0	0.27.1
pixelatorES	0.10.1	0.10.4

Parameters

Old parameter	New parameter
	--pna_denoise_run_ace_denoising
	--pna_denoise_ace_k
	--pna_denoise_run_pls_denoising
	--pna_denoise_pls_score_threshold

[github-actions]

nf-core pipelines lint overall result: Passed ✅ ⚠️

Posted for pipeline commit 00da840

+| ✅ 231 tests passed       |+
#| ❔   9 tests were ignored |#
!| ❗   2 tests had warnings |!

Details

❗ Test warnings:

• pipeline_if_empty_null - ifEmpty(null) found in /home/runner/work/pixelator/pixelator/lint_results.md: _* pipeline_if_empty_null - No ifEmpty(null) strings found
  _
• included_configs - Pipeline config does not include custom configs. Please add the includeConfig line.

❔ Tests ignored:

• files_exist - File is ignored: assets/multiqc_config.yml
• files_exist - File is ignored: conf/igenomes.config
• files_exist - File is ignored: conf/igenomes_ignored.config
• files_exist - File is ignored: tests/default.nf.test
• files_unchanged - File ignored due to lint config: LICENSE or LICENSE.md or LICENCE or LICENCE.md
• files_unchanged - File ignored due to lint config: .github/PULL_REQUEST_TEMPLATE.md
• files_unchanged - File ignored due to lint config: assets/nf-core-pixelator_logo_light.png
• files_unchanged - File ignored due to lint config: .gitignore or .prettierignore
• multiqc_config - multiqc_config

✅ Tests passed:

• files_exist - File found: .gitattributes
• files_exist - File found: .gitignore
• files_exist - File found: .nf-core.yml
• files_exist - File found: .prettierignore
• files_exist - File found: .prettierrc.yml
• files_exist - File found: CHANGELOG.md
• files_exist - File found: CITATIONS.md
• files_exist - File found: CODE_OF_CONDUCT.md
• files_exist - File found: LICENSE or LICENSE.md or LICENCE or LICENCE.md
• files_exist - File found: nextflow_schema.json
• files_exist - File found: nextflow.config
• files_exist - File found: README.md
• files_exist - File found: .github/.dockstore.yml
• files_exist - File found: .github/ISSUE_TEMPLATE/bug_report.yml
• files_exist - File found: .github/ISSUE_TEMPLATE/config.yml
• files_exist - File found: .github/ISSUE_TEMPLATE/feature_request.yml
• files_exist - File found: .github/PULL_REQUEST_TEMPLATE.md
• files_exist - File found: .github/workflows/branch.yml
• files_exist - File found: .github/workflows/nf-test.yml
• files_exist - File found: .github/actions/get-shards/action.yml
• files_exist - File found: .github/actions/nf-test/action.yml
• files_exist - File found: .github/workflows/linting_comment.yml
• files_exist - File found: .github/workflows/linting.yml
• files_exist - File found: assets/email_template.html
• files_exist - File found: assets/email_template.txt
• files_exist - File found: assets/sendmail_template.txt
• files_exist - File found: assets/nf-core-pixelator_logo_light.png
• files_exist - File found: conf/modules.config
• files_exist - File found: conf/test.config
• files_exist - File found: conf/test_full.config
• files_exist - File found: docs/CONTRIBUTING.md
• files_exist - File found: docs/images/nf-core-pixelator_logo_light.png
• files_exist - File found: docs/images/nf-core-pixelator_logo_dark.png
• files_exist - File found: docs/output.md
• files_exist - File found: docs/README.md
• files_exist - File found: docs/README.md
• files_exist - File found: docs/usage.md
• files_exist - File found: nf-test.config
• files_exist - File found: main.nf
• files_exist - File found: conf/base.config
• files_exist - File found: .github/workflows/awstest.yml
• files_exist - File found: .github/workflows/awsfulltest.yml
• files_exist - File found: modules.json
• files_exist - File found: ro-crate-metadata.json
• files_exist - File not found check: .github/ISSUE_TEMPLATE/bug_report.md
• files_exist - File not found check: .github/ISSUE_TEMPLATE/feature_request.md
• files_exist - File not found check: .github/workflows/push_dockerhub.yml
• files_exist - File not found check: .markdownlint.yml
• files_exist - File not found check: .nf-core.yaml
• files_exist - File not found check: .yamllint.yml
• files_exist - File not found check: bin/markdown_to_html.r
• files_exist - File not found check: conf/aws.config
• files_exist - File not found check: docs/images/nf-core-pixelator_logo.png
• files_exist - File not found check: lib/Checks.groovy
• files_exist - File not found check: lib/Completion.groovy
• files_exist - File not found check: lib/NfcoreTemplate.groovy
• files_exist - File not found check: lib/Utils.groovy
• files_exist - File not found check: lib/Workflow.groovy
• files_exist - File not found check: lib/WorkflowMain.groovy
• files_exist - File not found check: lib/WorkflowPixelator.groovy
• files_exist - File not found check: parameters.settings.json
• files_exist - File not found check: pipeline_template.yml
• files_exist - File not found check: Singularity
• files_exist - File not found check: lib/nfcore_external_java_deps.jar
• files_exist - File not found check: .travis.yml
• nextflow_config - Found nf-schema plugin
• nextflow_config - Config variable found: manifest.name
• nextflow_config - Config variable found: manifest.nextflowVersion
• nextflow_config - Config variable found: manifest.description
• nextflow_config - Config variable found: manifest.version
• nextflow_config - Config variable found: manifest.homePage
• nextflow_config - Config variable found: timeline.enabled
• nextflow_config - Config variable found: trace.enabled
• nextflow_config - Config variable found: report.enabled
• nextflow_config - Config variable found: dag.enabled
• nextflow_config - Config variable found: process.cpus
• nextflow_config - Config variable found: process.memory
• nextflow_config - Config variable found: process.time
• nextflow_config - Config variable found: params.outdir
• nextflow_config - Config variable found: params.input
• nextflow_config - Config variable found: manifest.mainScript
• nextflow_config - Config variable found: timeline.file
• nextflow_config - Config variable found: trace.file
• nextflow_config - Config variable found: report.file
• nextflow_config - Config variable found: dag.file
• nextflow_config - Config variable (correctly) not found: params.nf_required_version
• nextflow_config - Config variable (correctly) not found: params.container
• nextflow_config - Config variable (correctly) not found: params.singleEnd
• nextflow_config - Config variable (correctly) not found: params.igenomesIgnore
• nextflow_config - Config variable (correctly) not found: params.name
• nextflow_config - Config variable (correctly) not found: params.enable_conda
• nextflow_config - Config variable (correctly) not found: params.max_cpus
• nextflow_config - Config variable (correctly) not found: params.max_memory
• nextflow_config - Config variable (correctly) not found: params.max_time
• nextflow_config - Config variable (correctly) not found: params.validationFailUnrecognisedParams
• nextflow_config - Config variable (correctly) not found: params.validationLenientMode
• nextflow_config - Config variable (correctly) not found: params.validationSchemaIgnoreParams
• nextflow_config - Config variable (correctly) not found: params.validationShowHiddenParams
• nextflow_config - Config variable (correctly) not found: validation.failUnrecognisedParams
• nextflow_config - Config variable (correctly) not found: validation.failUnrecognisedHeaders
• nextflow_config - Config timeline.enabled had correct value: true
• nextflow_config - Config report.enabled had correct value: true
• nextflow_config - Config trace.enabled had correct value: true
• nextflow_config - Config dag.enabled had correct value: true
• nextflow_config - Config manifest.name began with nf-core/
• nextflow_config - Config variable manifest.homePage began with https://github.com/nf-core/
• nextflow_config - Config dag.file ended with .html
• nextflow_config - Config variable manifest.nextflowVersion started with >= or !>=
• nextflow_config - Config manifest.version does not contain dev for release: 4.1.0
• nextflow_config - Config params.custom_config_version is set to master
• nextflow_config - Config params.custom_config_base is set to https://raw.githubusercontent.com/nf-core/configs/master
• nextflow_config - Lines for loading custom profiles found
• nextflow_config - nextflow.config contains configuration profile test
• nextflow_config - Config default value correct: params.pna_amplicon_mismatches= 0.1
• nextflow_config - Config default value correct: params.pna_amplicon_remove_polyg= true
• nextflow_config - Config default value correct: params.pna_amplicon_quality_cutoff= 20
• nextflow_config - Config default value correct: params.pna_amplicon_low_complexity_filter= true
• nextflow_config - Config default value correct: params.pna_amplicon_low_complexity_threshold= 0.8
• nextflow_config - Config default value correct: params.pna_amplicon_lbs_filter= true
• nextflow_config - Config default value correct: params.pna_amplicon_lbs_filter_min_overlap= 8
• nextflow_config - Config default value correct: params.pna_amplicon_lbs_filter_error_rate= 0.1
• nextflow_config - Config default value correct: params.pna_demux_mismatches= 1
• nextflow_config - Config default value correct: params.pna_demux_output_chunk_reads= 50M
• nextflow_config - Config default value correct: params.pna_demux_output_max_chunks= 8
• nextflow_config - Config default value correct: params.pna_demux_strategy= independent
• nextflow_config - Config default value correct: params.pna_collapse_algorithm= directional
• nextflow_config - Config default value correct: params.pna_collapse_mismatches= 2
• nextflow_config - Config default value correct: params.pna_graph_multiplet_recovery= true
• nextflow_config - Config default value correct: params.pna_graph_initial_stage_leiden_resolution= 1.0
• nextflow_config - Config default value correct: params.pna_graph_refinement_stage_leiden_resolution= 0.01
• nextflow_config - Config default value correct: params.pna_graph_edge_cycle_verification= true
• nextflow_config - Config default value correct: params.pna_graph_min_count= 1.0
• nextflow_config - Config default value correct: params.pna_graph_max_refinement_recursion_depth= 3
• nextflow_config - Config default value correct: params.pna_graph_initial_stage_max_edges_to_remove= 10
• nextflow_config - Config default value correct: params.pna_graph_refinement_stage_max_edges_to_remove= 10
• nextflow_config - Config default value correct: params.pna_graph_component_size_min_threshold= 8000
• nextflow_config - Config default value correct: params.pna_sample_calling_remove_incompatible= true
• nextflow_config - Config default value correct: params.pna_sample_calling_confidence_threshold= 0.9
• nextflow_config - Config default value correct: params.pna_denoise_pval_threshold= 0.05
• nextflow_config - Config default value correct: params.pna_denoise_inflate_factor= 1.5
• nextflow_config - Config default value correct: params.pna_denoise_run_ace_denoising= true
• nextflow_config - Config default value correct: params.pna_denoise_ace_k= 3
• nextflow_config - Config default value correct: params.pna_denoise_run_pls_denoising= true
• nextflow_config - Config default value correct: params.pna_denoise_pls_score_threshold= -3.0
• nextflow_config - Config default value correct: params.pna_analysis_compute_proximity= true
• nextflow_config - Config default value correct: params.pna_analysis_compute_k_cores= true
• nextflow_config - Config default value correct: params.pna_analysis_proximity_nbr_of_permutations= 100
• nextflow_config - Config default value correct: params.pna_analysis_compute_svd_var_explained= true
• nextflow_config - Config default value correct: params.pna_analysis_svd_nbr_of_pivots= 50
• nextflow_config - Config default value correct: params.pna_layout_layout_algorithm= wpmds_3d
• nextflow_config - Config default value correct: params.pna_layout_pmds_pivots= 50
• nextflow_config - Config default value correct: params.pna_layout_wpmds_k= 5
• nextflow_config - Config default value correct: params.save_json= true
• nextflow_config - Config default value correct: params.custom_config_version= master
• nextflow_config - Config default value correct: params.custom_config_base= https://raw.githubusercontent.com/nf-core/configs/master
• nextflow_config - Config default value correct: params.publish_dir_mode= copy
• nextflow_config - Config default value correct: params.validate_params= true
• nextflow_config - Config default value correct: params.pipelines_testdata_base_path= https://raw.githubusercontent.com/nf-core/test-datasets/pixelator/
• nextflow_config - Config default value correct: params.modules_testdata_base_path= https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/
• nf_test_content - 'tests/proxiome_v2.nf.test' contains outdir parameter
• nf_test_content - 'tests/proxiome_v2.nf.test' snapshots a 'versions.yml' file
• nf_test_content - 'tests/proxiome_v2.nf.test' snapshots a 'versions.yml' file
• nf_test_content - 'tests/proxiome_v2.nf.test' contains outdir parameter
• nf_test_content - 'tests/proxiome_v2.nf.test' snapshots a 'versions.yml' file
• nf_test_content - 'tests/proxiome_v1.nf.test' contains outdir parameter
• nf_test_content - 'tests/proxiome_v1.nf.test' snapshots a 'versions.yml' file
• nf_test_content - 'tests/proxiome_v1.nf.test' snapshots a 'versions.yml' file
• nf_test_content - 'tests/proxiome_v1.nf.test' contains outdir parameter
• nf_test_content - 'tests/proxiome_v1.nf.test' snapshots a 'versions.yml' file
• nf_test_content - 'tests/nextflow.config' contains pipelines_testdata_base_path
• nf_test_content - 'tests/nextflow.config' contains modules_testdata_base_path
• nf_test_content - 'nf-test.config' sets a testsDir
• nf_test_content - 'nf-test.config' sets a workDir
• nf_test_content - 'nf-test.config' sets a configFile
• files_unchanged - .gitattributes matches the template
• files_unchanged - .prettierrc.yml matches the template
• files_unchanged - CODE_OF_CONDUCT.md matches the template
• files_unchanged - .github/.dockstore.yml matches the template
• files_unchanged - .github/ISSUE_TEMPLATE/bug_report.yml matches the template
• files_unchanged - .github/ISSUE_TEMPLATE/config.yml matches the template
• files_unchanged - .github/ISSUE_TEMPLATE/feature_request.yml matches the template
• files_unchanged - .github/workflows/branch.yml matches the template
• files_unchanged - .github/workflows/linting_comment.yml matches the template
• files_unchanged - .github/workflows/linting.yml matches the template
• files_unchanged - assets/email_template.html matches the template
• files_unchanged - assets/email_template.txt matches the template
• files_unchanged - assets/sendmail_template.txt matches the template
• files_unchanged - docs/images/nf-core-pixelator_logo_light.png matches the template
• files_unchanged - docs/images/nf-core-pixelator_logo_dark.png matches the template
• files_unchanged - docs/README.md matches the template
• actions_nf_test - '.github/workflows/nf-test.yml' is triggered on expected events
• actions_nf_test - '.github/workflows/nf-test.yml' checks minimum NF version
• actions_awstest - '.github/workflows/awstest.yml' is triggered correctly
• actions_awsfulltest - .github/workflows/awsfulltest.yml is triggered correctly
• actions_awsfulltest - .github/workflows/awsfulltest.yml does not use -profile test
• readme - README Nextflow minimum version badge matched config. Badge: 25.10.4, Config: 25.10.4
• readme - README nf-core template version badge found.
• readme - README Zenodo placeholder was replaced with DOI.
• pipeline_todos - No TODO strings found
• plugin_includes - No wrong validation plugin imports have been found
• pipeline_name_conventions - Name adheres to nf-core convention
• template_strings - Did not find any Jinja template strings (0 files)
• schema_lint - Schema lint passed
• schema_lint - Schema title + description lint passed
• schema_lint - Input mimetype lint passed: 'text/csv'
• schema_params - Schema matched params returned from nextflow config
• system_exit - No System.exit calls found
• actions_schema_validation - Workflow validation passed: clean-up.yml
• actions_schema_validation - Workflow validation passed: release-announcements.yml
• actions_schema_validation - Workflow validation passed: template-version-comment.yml
• actions_schema_validation - Workflow validation passed: awsfulltest.yml
• actions_schema_validation - Workflow validation passed: download_pipeline.yml
• actions_schema_validation - Workflow validation passed: linting.yml
• actions_schema_validation - Workflow validation passed: branch.yml
• actions_schema_validation - Workflow validation passed: nf-test.yml
• actions_schema_validation - Workflow validation passed: fix_linting.yml
• actions_schema_validation - Workflow validation passed: awstest.yml
• actions_schema_validation - Workflow validation passed: linting_comment.yml
• merge_markers - No merge markers found in pipeline files
• modules_json - Only installed modules found in modules.json
• modules_structure - modules directory structure is correct 'modules/nf-core/TOOL/SUBTOOL'
• local_component_structure - local subworkflows directory structure is correct 'subworkflows/local/TOOL/SUBTOOL'
• base_config - conf/base.config found and not ignored.
• modules_config - conf/modules.config found and not ignored.
• modules_config - PIXELATOR found in conf/modules.config and Nextflow scripts.
• modules_config - PIXELATOR_LIST_OPTIONS found in conf/modules.config and Nextflow scripts.
• modules_config - CAT_FASTQ found in conf/modules.config and Nextflow scripts.
• nfcore_yml - Repository type in .nf-core.yml is valid: pipeline
• nfcore_yml - nf-core version in .nf-core.yml is set to the latest version: 4.0.2
• rocrate_readme_sync - RO-Crate descriptions are in sync with README.md.
• version_consistency - Version tags are consistent: manifest.version = 4.1.0, nfcore_yml.version = 4.1.0

Run details

• nf-core/tools version 4.0.2
• Run at 2026-05-28 08:55:43

[ptajvar]

LGTM!