Interactive research manuscript and supplementary data visualisations for the study on Mtb replication dynamics by Nathan J. Day et al. (The Francis Crick Institute).
This web-based manuscript presents a high-throughput, live-cell imaging approach to quantify M. tuberculosis replication within human macrophages at single-cell resolution. The interface features high-resolution zoomable imaging data, interactive single-cell data visualisations (Plotly.js), and embedded supplementary videos of live-cell timelapse acquisitions.
The analysis code for image tiling, macrophage segmentation, tracking, and intracellular Mtb doubling time measurement is maintained separately at github.com/nthndy/macrohet.
Nathan J. Day & Maximiliano G. Gutierrez (corresponding author), Host-Pathogen Interactions in Tuberculosis Laboratory, The Francis Crick Institute. See the manuscript for the complete author list and affiliations.