Skip to content

README.md

Latest commit

 

History

History
31 lines (20 loc) · 919 Bytes

README.md

File metadata and controls

31 lines (20 loc) · 919 Bytes

pHe-analysis

Scripts for analysis of pHe Data

pHViability.R

This R script takes counted nuclei (DAPI-total, FITC-dead via SYTOX Green) and arranges them such that hypothesis testing and plotting can be done with minimal intervention in spreadsheet software.

totalCellCount.R

This R script is for the most part identical to pHViability.R, but has the following changes

  • Rather than analyze percent viability, we are analyzing the total cell count per image frame

This is useful if you need to see if cells are lysing/detaching due to a condition.

gapClosure.R

Quantification of migration during gap closure assay.

nhe1Active.pml

PyMOL script to render a PDB (7DSV) of NHE1 with the following

  • Bound calcineurin removed
  • Subunits of the dimer colored gray/tv_green
  • C-Terminal tails colored cyan/tv_blue
  • Cartoon-style rendering (Goodsell-like)