Scripts for analysis of pHe Data
This R script takes counted nuclei (DAPI-total, FITC-dead via SYTOX Green) and arranges them such that hypothesis testing and plotting can be done with minimal intervention in spreadsheet software.
This R script is for the most part identical to pHViability.R, but has the following changes
- Rather than analyze percent viability, we are analyzing the total cell count per image frame
This is useful if you need to see if cells are lysing/detaching due to a condition.
Quantification of migration during gap closure assay.
PyMOL script to render a PDB (7DSV) of NHE1 with the following
- Bound calcineurin removed
- Subunits of the dimer colored gray/tv_green
- C-Terminal tails colored cyan/tv_blue
- Cartoon-style rendering (Goodsell-like)