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GFP-Cell-Detection

DOI

Run the script 'sh run_script.sh $brainID' get the results.

Code Repo for the paper :

High precision automated detection of labeled nuclei in Gigapixel resolution image data of Mouse Brain

Sukhendu Das, Jaikishan Jayakumar, Samik Banerjee, Janani Ramaswamy, Venu Vangala, Keerthi Ram, and Partha Mitra.

BioRxiv (2019): 252247.

Configurations

Store the input Brain images in JP2 format with the alphabet F (to represent fluoroscent section) within the name in a folder with the brainID.

If you have Brain Region Segmentation (as .mat files) store that in the folder $brainID/reg_high_seg_pad/

If you have Brain Region Segmentation in other format, read the segmenation appropiately so that it represents a single-channel image in line 28 of GFP_Cell_Detection/main_bam.m

If you donot have Brain Region Segmentation, comment out line 28 of GFP_Cell_Detection/main_bam.m and add a line to create a white single-channel image of the same size as your input image.

The output is in GeoJSON format with the center locations marked as (y, -x) correponding to the (x,y) co-ordinate in the corresponding image

The names of the input image, Brain region segmentation map, output GeoJson will be the same as the input image, with same/different extensions.

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