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Biofilm-Viability-Checker

//This macro processes multiple images in a folder, outputting the number of bacteria pixels in the red channel and the green channel respectively to calculate the //viability of a biofilm stained with red and green viability stains. //It also saves overlay images showing the bacteria which have been detected. //See "Supplementary Information" file for details on how to run and implement the macro.

//Created by Sophie Mountcastle (sem093@bham.ac.uk) and Nina Vyas (n.vyas@bham.ac.uk) //University of Birmingham, Edgbaston, B15 2TT, UK //For queries please contact Dr Sarah Kuehne (s.a.kuehne@bham.ac.uk)

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This macro writtin for use with Fiji (ImageJ) processes multiple confocal images of live/dead stained biofilms, outputting the number of bacteria pixels in the red channel and both channels respectively to calculate the viability of the biofilm. It also saves overlay images showing the bacteria which have been detected.

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