Skip to content

summary - PCA done for group of samples not for samples, time points #163

@mmaslon

Description

@mmaslon

I am trying to run PCA analysis with alleyoop summary. I have several samples, e.g. LKO rep1 0', LKO rep2 0' ..... MUT1 rep1 0', MUT rep2 0' .... MUT2 rep1 0', MUT2 rep2 0',
When I run the summary, I get the PCA plots for LKO vs MUT1 vs MUT2, rather than individual samples (e..g. I would rather see how same time points look for different conditions). I am not sure what I am doing wrong,
my code is"

alleyoop summary -o $output_dir/summary -t $tcount $bam/*.bam

head of tcount file looks like this:

#slamdunk v0.4.3	3	sample info:	LKO	8	rep3	120

Can you please suggest what could be going wrong? I think this starts with the sample info, the info on replica and time poiint is somehow not taken into account.

Thanks

Metadata

Metadata

Assignees

No one assigned

    Labels

    No labels
    No labels

    Projects

    No projects

    Milestone

    No milestone

    Relationships

    None yet

    Development

    No branches or pull requests

    Issue actions