seurat-v5

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mianaz / srtdisk

Seurat v5 compatible HDF5 converter for single-cell data — h5ad, h5Seurat, h5mu, Loom with full spatial data support

bioinformatics hdf5 r-package scrnaseq single-cell-genomics single-cell-rna-seq seurat spatial-transcriptomics anndata h5ad mudata seurat-v5 h5mu

Updated Apr 1, 2026
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HABIOTECH / seurat-pbmc3k-SingleCellAnalysis-walkthrough

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Annotated Seurat v5 walkthrough of the 10x Genomics PBMC 3k dataset: QC, normalization, feature selection, PCA, graph-based clustering, UMAP, marker discovery, and annotation of 2,638 cells into 8 immune populations. Each step documents parameter rationale and common failure modes. R 4.4.2 / Seurat 5.5.1.

bioinformatics-pipeline single-cell-rna-seq clustering-analysis umap2 pbmc3k seurat-v5