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fix(qc): expand Met oxidation flag to recommend Nle substitution - #93
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All 4 SEED-007 pilot candidates contain Met at position 6. The presynth QC flag now explicitly recommends Nle (norleucine) substitution for oxidation-resistant synthesis and makes the HPLC purity check mandatory, not advisory. This surfaces the most actionable synthesis guidance at the point where the wet-lab team reads the QC report.
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Summary
Improves the Met (methionine) presynth QC flag message to explicitly recommend Nle (norleucine) substitution for oxidation-resistant synthesis.
Before:
MET×1: oxidation risk — store at −80°C, check purity pre-assayAfter:
MET×1: oxidation risk — store at −80°C under inert atmosphere; request Nle (norleucine) substitution for oxidation-resistant synthesis; mandatory HPLC purity check at receiptMotivation
All 4 SEED-007 (Bombolitin-II) pilot panel candidates contain Met at position 6 (
IKFTTMLRKLG,AKITTMLKKLG,IKISTMLKKAG,IKITTMAKKVG). Oxidised Met (Met→Met-sulfoxide) causes hydrophobic collapse and loss of amphipathicity, potentially invalidating the assay result. The wet-lab handoff doc (PR #100) already recommends Nle substitution explicitly; this change surfaces the same guidance at the QC report level where the synthesis order is prepared.Test plan
test_met_flag_in_flags_listchecks"MET" in f, not exact message; still passescheck_panel([{'candidate_id': 'SEED-007_VAR_009', 'sequence': 'IKFTTMLRKLG'}])produces correct new message🤖 Generated with Claude Code