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fix(qc): expand Met oxidation flag to recommend Nle substitution #93

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Merged
cschanhniem merged 1 commit into from
Jun 28, 2026
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fix(qc): expand Met oxidation flag to recommend Nle substitution #93

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6 changes: 5 additions & 1 deletion src/openamp_foundry/qc/presynth_check.py
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Original file line number Diff line number Diff line change
Expand Up @@ -346,7 +346,11 @@ def check_sequence(candidate_id: str, seq: str, mu_h: float = 0.0) -> SynthQC:
if qc.cysteine_count > 0:
qc.flags.append(f"CYSTEINE×{qc.cysteine_count}: disulfide/oxidation risk — store under N₂")
if qc.methionine_count > 0:
qc.flags.append(f"MET×{qc.methionine_count}: oxidation risk — store at −80°C, check purity pre-assay")
qc.flags.append(
f"MET×{qc.methionine_count}: oxidation risk — store at −80°C under inert atmosphere; "
"request Nle (norleucine) substitution for oxidation-resistant synthesis; "
"mandatory HPLC purity check at receipt"
)
if qc.aggregation_risk:
qc.flags.append(f"HYDROPHOBIC_RUN ({qc.hydrophobic_run}): aggregation risk — check solubility at 1 mM")
if len(qc.trypsin_sites) > 2:
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