Skip to content
Open
Show file tree
Hide file tree
Changes from all commits
Commits
File filter

Filter by extension

Filter by extension


Conversations
Failed to load comments.
Loading
Jump to
Jump to file
Failed to load files.
Loading
Diff view
Diff view
6 changes: 3 additions & 3 deletions README.md
Original file line number Diff line number Diff line change
Expand Up @@ -79,7 +79,7 @@ To get single cell VR report for Seurat scRNA-seq analysis:
scvr -f ./seurat_result/seurat3d_10xpbmc.loom -t seurat -a annotations.txt -g genes.txt -o seurat_report
```
* Input files can be found [here](https://www.dropbox.com/sh/tpk4qfm5qsjpffn/AADmKmyDx7rhzKBOpIlAgMEUa?dl=0)
* To generate the `seurat3d_10xpbmc.loom`, check out [Seurat analysis](https://nbviewer.jupyter.org/github/pinellolab/singlecellvr/blob/master/examples/seurat_10xpbmc.ipynb?flush_cache=true). *(Make sure set `n.components = 3` in `pbmc <- RunUMAP(pbmc, dims = 1:10, n.components = 3)`)*
* To generate the `seurat3d_10xpbmc.loom`, check out [Seurat analysis](https://nbviewer.jupyter.org/github/pinellolab/singlecellvr/blob/master/examples/seurat_10xpbmc.ipynb?flush_cache=true). *(Make sure set `n.components = 3` in `pbmc <- RunUMAP(pbmc, dims = 1:10, n.components = 3)`)*. NOTE the notebook is designed for Seurat 3.x based analysis, if you have installed Seurat 4.0.x, please use the [Seurat4 analysis](https://github.com/qinqian/singlecellvr/blob/master/examples/seurat4_pbmc3k.ipynb).

To get single cell VR report for Seurat scRNA-seq integration analysis:
```bash
Expand All @@ -90,9 +90,9 @@ scvr -f ./seurat_integration_result/seurat3d_integration.loom -t seurat -a annot
### Velocity:
To get single cell velocity report for scvelo:
``` bash
scvr -t velocity -f pancrease_velocity.h5ad -a clusters
scvr -t velocity -f pancrease_velocity_updated.h5ad -a clusters
```
* To generate the `pancrease_velocity.h5ad`, check out examples/velocity_3d.ipynb.
* To generate the `pancrease_velocity_updated.h5ad`, check out examples/velocity_3d.ipynb and examples/velocity_3d_scale.ipynb.

### STREAM:
To get single cell VR report for STREAM :
Expand Down
1 change: 0 additions & 1 deletion dash_app/apps/dash-singlecell-vr/app.py
Original file line number Diff line number Diff line change
Expand Up @@ -762,4 +762,3 @@ def update_output(uploaded_filenames, uploaded_file_contents):
if __name__ == "__main__":
# app.run_server(port=os.environ['PORT'])
app.run_server(port=8050,debug=True,host='0.0.0.0',ssl_context='adhoc')

Loading