Unsupervised Illumina EPIC v2 methylation array pipeline (QC, NOOB normalization, PCA, hierarchical clustering, heatmap, MDS, and UMAP from raw IDAT files)
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Updated
Mar 18, 2026 - R
Unsupervised Illumina EPIC v2 methylation array pipeline (QC, NOOB normalization, PCA, hierarchical clustering, heatmap, MDS, and UMAP from raw IDAT files)
Reproducible cross-array harmonisation and region detection for longitudinal EWAS (Illumina 450K + EPIC), extending the EWASGalaxy suite
A hands-on, eleven-chapter Quarto tutorial for epigenome-wide association studies: raw IDATs through QC, normalization, probe filtering on the Zhou EPIC v8.1 mask, cell composition, batch/latent structure, limma+BACON EWAS, a Snakemake pipeline run, and functional annotation.
EPIC methylation analysis of OED-to-OSCC progression integrating grade-adjusted modeling, spatial candidates, exposure-associated signatures, and longitudinal lesion trajectories.
GSE142512 450K and EPIC DNA methylation resources for Bioconductor ExperimentHub. DOI: 10.5281/zenodo.21198533
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